Development of novel osteochondral scaffolds and related in vitro environment with the aid of chemical engineering principles
Само за регистроване кориснике
2024
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
In tissue engineering, collaboration among experts from different fields is needed to design appropriate
cell scaffolds and the required 3D environment. Osteochondral tissue engineering is particularly
challenging due to the necessity to provide scaffolds that imitate structural and compositional
differences of two neighboring tissues, articular cartilage and bone, and the required complex
biophysical environments to cultivate such scaffolds. This work focuses on two key objectives: first, to
develop bilayered osteochondral scaffolds based on gellan gum and bioactive glass, and second, to
create a biomimetic environment for scaffold characterization by designing and utilization of novel
dual-medium cultivation bioreactor chambers. Basic chemical engineering principles were utilized to
aid both aims. First, a simple heat transport model based on one-dimensional conduction was applied
as a guideline for bilayered scaffold preparation, leading to the formation of the gelatinous u...pper part
and a macroporous lower part with a thin, well-integrated interfacial zone. Second, a novel cultivation
chamber was developed to be used in a dynamic compression bioreactor to provide possibilities for
flow of two different media, such as chondrogenic and osteogenic. These chambers were utilized for
characterization of the novel scaffolds regarding bioactivity and stability under dynamic compression
and fluid perfusion during 14 days, while flow distribution under different conditions was analyzed by
a tracer method and residence time distribution analysis.
Кључне речи:
osteochondral tissue engineering / bilayered scaffolds / biomimetic bioreactor / RTD analysisИзвор:
Biomedical Materials, 2024Издавач:
- IOP Publishing Ltd.
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
- European Union’s H2020-WIDESPREAD-2020-5, Twinning to excel materials engineering for medical devices, under grant agreement No. 952033
Напомена:
- This is the Accepted Manuscript version of an article accepted for publication in Biomedical Materials. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at https://doi.org/10.1088/1748-605X/ad6ac1
- This is the peer-reviewed version of the following article: Zvicer J, Milošević M, Medić A, Novak S, Obradović B. Development of novel osteochondral scaffolds and related in vitro environment with the aid of chemical engineering principles. in Biomedical Materials. 2024. https://doi.org/10.1088/1748-605X/ad6ac1
- Published version: https://technorep.tmf.bg.ac.rs/handle/123456789/7614
Повезане информације:
- Верзија документа
https://technorep.tmf.bg.ac.rs/handle/123456789/7614 - Верзија документа
https://doi.org/10.1088/1748-605X/ad6ac1
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Zvicer, Jovana AU - Milošević, Mia AU - Medić, Ana AU - Novak, Saša AU - Obradović, Bojana PY - 2024 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7571 AB - In tissue engineering, collaboration among experts from different fields is needed to design appropriate cell scaffolds and the required 3D environment. Osteochondral tissue engineering is particularly challenging due to the necessity to provide scaffolds that imitate structural and compositional differences of two neighboring tissues, articular cartilage and bone, and the required complex biophysical environments to cultivate such scaffolds. This work focuses on two key objectives: first, to develop bilayered osteochondral scaffolds based on gellan gum and bioactive glass, and second, to create a biomimetic environment for scaffold characterization by designing and utilization of novel dual-medium cultivation bioreactor chambers. Basic chemical engineering principles were utilized to aid both aims. First, a simple heat transport model based on one-dimensional conduction was applied as a guideline for bilayered scaffold preparation, leading to the formation of the gelatinous upper part and a macroporous lower part with a thin, well-integrated interfacial zone. Second, a novel cultivation chamber was developed to be used in a dynamic compression bioreactor to provide possibilities for flow of two different media, such as chondrogenic and osteogenic. These chambers were utilized for characterization of the novel scaffolds regarding bioactivity and stability under dynamic compression and fluid perfusion during 14 days, while flow distribution under different conditions was analyzed by a tracer method and residence time distribution analysis. PB - IOP Publishing Ltd. T2 - Biomedical Materials T1 - Development of novel osteochondral scaffolds and related in vitro environment with the aid of chemical engineering principles DO - 10.1088/1748-605X/ad6ac1 ER -
@article{ author = "Zvicer, Jovana and Milošević, Mia and Medić, Ana and Novak, Saša and Obradović, Bojana", year = "2024", abstract = "In tissue engineering, collaboration among experts from different fields is needed to design appropriate cell scaffolds and the required 3D environment. Osteochondral tissue engineering is particularly challenging due to the necessity to provide scaffolds that imitate structural and compositional differences of two neighboring tissues, articular cartilage and bone, and the required complex biophysical environments to cultivate such scaffolds. This work focuses on two key objectives: first, to develop bilayered osteochondral scaffolds based on gellan gum and bioactive glass, and second, to create a biomimetic environment for scaffold characterization by designing and utilization of novel dual-medium cultivation bioreactor chambers. Basic chemical engineering principles were utilized to aid both aims. First, a simple heat transport model based on one-dimensional conduction was applied as a guideline for bilayered scaffold preparation, leading to the formation of the gelatinous upper part and a macroporous lower part with a thin, well-integrated interfacial zone. Second, a novel cultivation chamber was developed to be used in a dynamic compression bioreactor to provide possibilities for flow of two different media, such as chondrogenic and osteogenic. These chambers were utilized for characterization of the novel scaffolds regarding bioactivity and stability under dynamic compression and fluid perfusion during 14 days, while flow distribution under different conditions was analyzed by a tracer method and residence time distribution analysis.", publisher = "IOP Publishing Ltd.", journal = "Biomedical Materials", title = "Development of novel osteochondral scaffolds and related in vitro environment with the aid of chemical engineering principles", doi = "10.1088/1748-605X/ad6ac1" }
Zvicer, J., Milošević, M., Medić, A., Novak, S.,& Obradović, B.. (2024). Development of novel osteochondral scaffolds and related in vitro environment with the aid of chemical engineering principles. in Biomedical Materials IOP Publishing Ltd... https://doi.org/10.1088/1748-605X/ad6ac1
Zvicer J, Milošević M, Medić A, Novak S, Obradović B. Development of novel osteochondral scaffolds and related in vitro environment with the aid of chemical engineering principles. in Biomedical Materials. 2024;. doi:10.1088/1748-605X/ad6ac1 .
Zvicer, Jovana, Milošević, Mia, Medić, Ana, Novak, Saša, Obradović, Bojana, "Development of novel osteochondral scaffolds and related in vitro environment with the aid of chemical engineering principles" in Biomedical Materials (2024), https://doi.org/10.1088/1748-605X/ad6ac1 . .