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The oxidation of anthraquinone dye using HRP immobilized as a cross-linked enzyme aggregates
Oksidacija antrahinonskih boja peroksidazom iz rena imobilisanom u obliku umreženih enzimskih agregata
dc.creator | Šekuljica, Nataša | |
dc.creator | Prlainović, Nevena | |
dc.creator | Stefanović, Andrea | |
dc.creator | Jovanović, Jelena | |
dc.creator | Jakovetić, Sonja | |
dc.creator | Knežević-Jugović, Zorica | |
dc.creator | Mijin, Dušan | |
dc.date.accessioned | 2021-03-10T12:59:44Z | |
dc.date.available | 2021-03-10T12:59:44Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 2406-2979 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3216 | |
dc.description.abstract | Finding a sustainable and ecofriendly methods for recalcitrant synthetic dyes removal is a researchers major challenge. A carrier-free technique for commercial HRP immobilization is a focus of the present study. The immobilized biocatalyst, HRP-CLEAs with 580 U g-1 of the activity was obtained under the following immobilization conditions: precipitation reagent 80% ammonium sulphate, cross-linking reagent 1% of glutaraldehyde and protein-fedder, bovine serum albumin (BSA) concentration 5 mg ml-1. The obtained HRP-CLEAs showed great affinity towards model anthraquinone dye, C. I. Acid Violet 109. 88.4% of the dye was oxidized under the reaction conditions: pH 4, dye concentration 40 mg l-1, H2O2 concentration 1 mM and 0.1 U of HRP-CLEAs. The possibility of the immobilized biocatalyst application in five and eight oxidation cycles of the dye and pyrogallol (retained activity 80%), respectively, indicates that HRP-CLEAs is an efficient and environmentally friendly biocatalyst with great potential in aromatic compounds removal from wastewater. This paper is a continuation of our earlier research related to HRP from horseradish extract immobilization in the form of CLEAs and the application in the wastewater colored with a synthetic anthraquinone dye treatment. | en |
dc.description.abstract | Pronalazak održivih i ekološki prihvatljivih metoda za uklanjanje sintetičkih boja je jedan od vodećih izazova za istraživače. U ovom radu akcenat je na metodi imobilizacije bez primene nosača. Imobilisani preparat peroksidaze iz rena, aktivnosti 580 U g-1 dobijen je pod sledećim uslovima imobilizacije: taložni reagens 80% amonijum-sulfat, umrežavajući reagens 1% glutaraldehid i goveđi serum albumin koncentracije 5 mg ml-1. Dobijeni umreženi agregat peroksidaze iz rena pokazao je veliki afinitet prema model antrahinonskoj boji, C. I. AV 109. Pod optimalnim uslovima: pH 4, koncentracja boje 40 mg-1, koncentracija H2O2 i 0,1 U enzimske aktivnosti oksidovano je 88,4% ispitivane boje. Mogućnost primene imobilisanog enzima kroz pet i osam uzastopnih ciklusa oksidacije boje i pirogalola (zadržana aktivnost 80%) pokazuje da je imobilisani enzim efikasan i ekološki prihvatljiv biokatalizator koji se može koristiti u oksidaciji aromatičnih jedinjenja iz otpadnih voda. Ovaj rad predstavlja nastavak istraživanja vezanog za umrežavanje peroksidaze iz svežeg ekstrakta rena, koja se takođe pokazala kao imobilisani biokatalizator sa velikim potencijalom primene u tretmanu obojenih otpadnih voda. | sr |
dc.publisher | Univerzitet u Nišu - Tehnološki fakultet, Leskovac | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172013/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172046/RS// | |
dc.rights | openAccess | |
dc.source | Advanced Technologies | |
dc.subject | horseradish peroxidase | en |
dc.subject | immobilization | en |
dc.subject | cross-linked enzyme aggregates | en |
dc.subject | anthraquinone dye | en |
dc.subject | wastewater | en |
dc.subject | peroksidaza iz rena | sr |
dc.subject | imobilizacija | sr |
dc.subject | umreženi enzimski agregati | sr |
dc.subject | antrahinonske boje | sr |
dc.subject | otpadne vode | sr |
dc.title | The oxidation of anthraquinone dye using HRP immobilized as a cross-linked enzyme aggregates | en |
dc.title | Oksidacija antrahinonskih boja peroksidazom iz rena imobilisanom u obliku umreženih enzimskih agregata | sr |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 26 | |
dc.citation.issue | 2 | |
dc.citation.other | 5(2): 18-26 | |
dc.citation.rank | M52 | |
dc.citation.spage | 18 | |
dc.citation.volume | 5 | |
dc.identifier.doi | 10.5937/savteh1602018S | |
dc.identifier.fulltext | http://TechnoRep.tmf.bg.ac.rs/bitstream/id/9412/2406-29791602018S.pdf | |
dc.type.version | publishedVersion |