Chemical Modification of Glycoproteins' Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase
Autori
Stanisic, Marija D.Popovic Kokar, Nikolina
Ristic, Predrag
Balaz, Ana Marija
Sencanski, Milan
Ognjanovic, Milos
Đokić, Veljko
Prodanovic, Radivoje
Todorovic, Tamara R.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Zeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to encapsulate proteins via biomimetic mineralization. The formation of nucleation centers and further biocomposite crystal growth is entirely governed by the pure electrostatic interactions between the protein's surface and the positively charged Zn(II) metal ions. It was previously shown that enhancing these electrostatic interactions by a chemical modification of surface amino acid residues can lead to a rapid biocomposite crystal formation. However, a chemical modification of carbohydrate components by periodate oxidation for glycoproteins can serve as an alternative strategy. In the present study, an industrially important enzyme glucose oxidase (GOx) was selected as a model system. Periodate oxidation of GOx by 2.5 mM sodium periodate increased negative charge on the enzyme molecule, from -10.2 to -36.9 mV, as shown by zeta potential measurements and native PAGE electrophoresis. Biomineralization expe...riments with oxidized GOx resulted in higher specific activity, effectiveness factor, and higher thermostability of the ZIF-8 biocomposites. Periodate oxidation of carbohydrate components for glycoproteins can serve as a facile and general method for facilitating the biomimetic mineralization of other industrially relevant glycoproteins.
Ključne reči:
metal-organic frameworks / ZIF-8 / biomimetic mineralization / biocomposites / biocatalystsIzvor:
Polymers, 2021, 13, 22, -3875
DOI: 10.3390/polym13223875
ISSN: 2073-4360
PubMed: 34833174
WoS: 000727517900001
Scopus: 2-s2.0-85119301454
Institucija/grupa
Inovacioni centarTY - JOUR AU - Stanisic, Marija D. AU - Popovic Kokar, Nikolina AU - Ristic, Predrag AU - Balaz, Ana Marija AU - Sencanski, Milan AU - Ognjanovic, Milos AU - Đokić, Veljko AU - Prodanovic, Radivoje AU - Todorovic, Tamara R. PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4817 AB - Zeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to encapsulate proteins via biomimetic mineralization. The formation of nucleation centers and further biocomposite crystal growth is entirely governed by the pure electrostatic interactions between the protein's surface and the positively charged Zn(II) metal ions. It was previously shown that enhancing these electrostatic interactions by a chemical modification of surface amino acid residues can lead to a rapid biocomposite crystal formation. However, a chemical modification of carbohydrate components by periodate oxidation for glycoproteins can serve as an alternative strategy. In the present study, an industrially important enzyme glucose oxidase (GOx) was selected as a model system. Periodate oxidation of GOx by 2.5 mM sodium periodate increased negative charge on the enzyme molecule, from -10.2 to -36.9 mV, as shown by zeta potential measurements and native PAGE electrophoresis. Biomineralization experiments with oxidized GOx resulted in higher specific activity, effectiveness factor, and higher thermostability of the ZIF-8 biocomposites. Periodate oxidation of carbohydrate components for glycoproteins can serve as a facile and general method for facilitating the biomimetic mineralization of other industrially relevant glycoproteins. T2 - Polymers T1 - Chemical Modification of Glycoproteins' Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase EP - 3875 IS - 22 VL - 13 DO - 10.3390/polym13223875 ER -
@article{ author = "Stanisic, Marija D. and Popovic Kokar, Nikolina and Ristic, Predrag and Balaz, Ana Marija and Sencanski, Milan and Ognjanovic, Milos and Đokić, Veljko and Prodanovic, Radivoje and Todorovic, Tamara R.", year = "2021", abstract = "Zeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to encapsulate proteins via biomimetic mineralization. The formation of nucleation centers and further biocomposite crystal growth is entirely governed by the pure electrostatic interactions between the protein's surface and the positively charged Zn(II) metal ions. It was previously shown that enhancing these electrostatic interactions by a chemical modification of surface amino acid residues can lead to a rapid biocomposite crystal formation. However, a chemical modification of carbohydrate components by periodate oxidation for glycoproteins can serve as an alternative strategy. In the present study, an industrially important enzyme glucose oxidase (GOx) was selected as a model system. Periodate oxidation of GOx by 2.5 mM sodium periodate increased negative charge on the enzyme molecule, from -10.2 to -36.9 mV, as shown by zeta potential measurements and native PAGE electrophoresis. Biomineralization experiments with oxidized GOx resulted in higher specific activity, effectiveness factor, and higher thermostability of the ZIF-8 biocomposites. Periodate oxidation of carbohydrate components for glycoproteins can serve as a facile and general method for facilitating the biomimetic mineralization of other industrially relevant glycoproteins.", journal = "Polymers", title = "Chemical Modification of Glycoproteins' Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase", pages = "3875", number = "22", volume = "13", doi = "10.3390/polym13223875" }
Stanisic, M. D., Popovic Kokar, N., Ristic, P., Balaz, A. M., Sencanski, M., Ognjanovic, M., Đokić, V., Prodanovic, R.,& Todorovic, T. R.. (2021). Chemical Modification of Glycoproteins' Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase. in Polymers, 13(22). https://doi.org/10.3390/polym13223875
Stanisic MD, Popovic Kokar N, Ristic P, Balaz AM, Sencanski M, Ognjanovic M, Đokić V, Prodanovic R, Todorovic TR. Chemical Modification of Glycoproteins' Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase. in Polymers. 2021;13(22):null-3875. doi:10.3390/polym13223875 .
Stanisic, Marija D., Popovic Kokar, Nikolina, Ristic, Predrag, Balaz, Ana Marija, Sencanski, Milan, Ognjanovic, Milos, Đokić, Veljko, Prodanovic, Radivoje, Todorovic, Tamara R., "Chemical Modification of Glycoproteins' Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase" in Polymers, 13, no. 22 (2021), https://doi.org/10.3390/polym13223875 . .