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Cryopreservation of apple shoot tips by vitrification and subsequent plant regeneration
dc.creator | Vujovic, T. | |
dc.creator | Ruzic, D. | |
dc.creator | Cerović, Radosav | |
dc.date.accessioned | 2022-03-04T11:18:48Z | |
dc.date.available | 2022-03-04T11:18:48Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 0567-7572 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4831 | |
dc.description.abstract | This paper investigates the influence of composition of vitrification solution on the regrowth capacity of apple 'Gala Must' (Malus x domestica Borkh.) cryopreserved by a vitrification technique. In vitro-grown shoot tips were precultured in the dark at 23 degrees C, in a liquid MS medium with two sucrose treatments (0.3 M for 15 h, then 0.7 M for 5 h). After loading at room temperature with solution containing 2 M glycerol and 0.4 M sucrose for 20 min, explants were dehydrated at 0 degrees C for 30, 40 and 50 min. Dehydration was done using either original PVS2 (13.7% w/v sucrose, 30% w/v glycerol, 15% w/v ethylene glycol, 15% w/v DMSO) or modified PVS2 solution (PVS A3 - 22.5% w/v sucrose, 37.5% w/v glycerol, 15% w/v ethylene glycol and 15% w/v DMSO). The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 40 and 85 and 20-40%, respectively. The highest values of both parameters were achieved with longest treatment duration. Dehydration with the PVS A3 solution resulted in considerably higher survival rates (68.2-90%), as well as higher regrowth rates (15-75%) after cryopreservation. Fifty-min treatment with this vitrification solution led to a significant increase in the percentage of regrowth (up to 75%). After regrowth, shoots were successfully multiplied and rooted. These results prove the feasibility of the PVS A3 based vitrification technique for a long-term storage of this genotype. | en |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31064/RS// | |
dc.rights | restrictedAccess | |
dc.source | Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo | |
dc.subject | Malus x domestica Borkh. | en |
dc.subject | in vitro | en |
dc.subject | cryopreservation | en |
dc.subject | vitrification solutions | en |
dc.title | Cryopreservation of apple shoot tips by vitrification and subsequent plant regeneration | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 40 | |
dc.citation.other | 1308(): 33-40 | |
dc.citation.spage | 33 | |
dc.citation.volume | 1308 | |
dc.identifier.doi | 10.17660/ActaHortic.2021.1308.6 | |
dc.identifier.scopus | 2-s2.0-85105040617 | |
dc.identifier.wos | 000711941600006 | |
dc.type.version | publishedVersion |
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