Microbial hydrolytic enzymes are relevant biotechnological products that can be applied in various industries. In this study, we have tested the activity of hydrolytic enzymes of a newly isolated Hymenobacter sp. CKS3 strain and showed, for the first time, that members of Hymenobacter genus have still unexplored hydrolytic potential. Crude hydrolytic enzymes, produced by the strain CKS3 on a waste medium, were incorporated into a process of bioethanol production using waste bread. The conditions for bread hydrolysis were optimized using statistical design. Waste bread hydrolysate obtained under optimal conditions (100.73 h of hydrolysis, waste bread concentration 20.36% and agitation speed 200 rpm) contained 19.89 g/l of reducing sugars. A high performance liquid chromatography of hydrolyzed waste bread samples showed that the main components of the hydrolysate were dextrins, maltotriose, maltose and glucose. When using this substrate and waste baker's yeast for ethanol production unde...r non-optimized conditions 1.73% of ethanol was produced. The results of this study showed that a newly isolated Hymenobacter sp. CKS3 can be utilized for enzymatic hydrolysis and bioethanol production in a process relying on waste materials. Furthermore, it was demonstrated that members of Hymenobacter genus have a significant and currently unexplored potential for bio-based industrial applications.
TY - JOUR
AU - Mihajlovski, Katarina
AU - Rajilić-Stojanović, Mirjana
AU - Dimitrijević-Branković, Suzana
PY - 2020
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4536
AB - Microbial hydrolytic enzymes are relevant biotechnological products that can be applied in various industries. In this study, we have tested the activity of hydrolytic enzymes of a newly isolated Hymenobacter sp. CKS3 strain and showed, for the first time, that members of Hymenobacter genus have still unexplored hydrolytic potential. Crude hydrolytic enzymes, produced by the strain CKS3 on a waste medium, were incorporated into a process of bioethanol production using waste bread. The conditions for bread hydrolysis were optimized using statistical design. Waste bread hydrolysate obtained under optimal conditions (100.73 h of hydrolysis, waste bread concentration 20.36% and agitation speed 200 rpm) contained 19.89 g/l of reducing sugars. A high performance liquid chromatography of hydrolyzed waste bread samples showed that the main components of the hydrolysate were dextrins, maltotriose, maltose and glucose. When using this substrate and waste baker's yeast for ethanol production under non-optimized conditions 1.73% of ethanol was produced. The results of this study showed that a newly isolated Hymenobacter sp. CKS3 can be utilized for enzymatic hydrolysis and bioethanol production in a process relying on waste materials. Furthermore, it was demonstrated that members of Hymenobacter genus have a significant and currently unexplored potential for bio-based industrial applications.
PB - Pergamon-Elsevier Science Ltd, Oxford
T2 - Renewable Energy
T1 - Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production
EP - 633
SP - 627
VL - 152
DO - 10.1016/j.renene.2020.01.101
ER -
@article{
author = "Mihajlovski, Katarina and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2020",
abstract = "Microbial hydrolytic enzymes are relevant biotechnological products that can be applied in various industries. In this study, we have tested the activity of hydrolytic enzymes of a newly isolated Hymenobacter sp. CKS3 strain and showed, for the first time, that members of Hymenobacter genus have still unexplored hydrolytic potential. Crude hydrolytic enzymes, produced by the strain CKS3 on a waste medium, were incorporated into a process of bioethanol production using waste bread. The conditions for bread hydrolysis were optimized using statistical design. Waste bread hydrolysate obtained under optimal conditions (100.73 h of hydrolysis, waste bread concentration 20.36% and agitation speed 200 rpm) contained 19.89 g/l of reducing sugars. A high performance liquid chromatography of hydrolyzed waste bread samples showed that the main components of the hydrolysate were dextrins, maltotriose, maltose and glucose. When using this substrate and waste baker's yeast for ethanol production under non-optimized conditions 1.73% of ethanol was produced. The results of this study showed that a newly isolated Hymenobacter sp. CKS3 can be utilized for enzymatic hydrolysis and bioethanol production in a process relying on waste materials. Furthermore, it was demonstrated that members of Hymenobacter genus have a significant and currently unexplored potential for bio-based industrial applications.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Renewable Energy",
title = "Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production",
pages = "633-627",
volume = "152",
doi = "10.1016/j.renene.2020.01.101"
}
Mihajlovski, K., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2020). Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production. in Renewable Energy
Pergamon-Elsevier Science Ltd, Oxford., 152, 627-633.
https://doi.org/10.1016/j.renene.2020.01.101
Mihajlovski K, Rajilić-Stojanović M, Dimitrijević-Branković S. Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production. in Renewable Energy. 2020;152:627-633.
doi:10.1016/j.renene.2020.01.101 .
Mihajlovski, Katarina, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production" in Renewable Energy, 152 (2020):627-633,
https://doi.org/10.1016/j.renene.2020.01.101 . .
