TY  - JOUR
AU  - Babić Radić, Marija M.
AU  - Vukomanović, Marija
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7410
AB  - This study proposes synthesis and evaluation of gelatin-/alginate-based hydrogel scaffolds reinforced with titanium dioxide (TiO2) nanoparticles which, through their combination with allantoin, quercetin, and caffeic acid, provide multi-target therapy directed on all phases of the wound healing process. These scaffolds provide the simultaneous release of bioactive agents and concurrently support cell/tissue repair through the replicated structure of a native extracellular matrix. The hydrogel scaffolds were synthesized via a crosslinking reaction using EDC as a crosslinker for gelatin. Synthesized hydrogel scaffolds and the effect of TiO2 on their properties were characterized by structural, mechanical, morphological, and swelling properties, and the porosity, wettability, adhesion to skin tissue, and simultaneous release features. The biocompatibility of the scaffolds was tested in vitro on fibroblasts (MRC5 cells) and in vivo (Caenorhabditis elegans) in a survival probe. The scaffolds revealed porous interconnected morphology, porosity of 88.33 to 96.76%, elastic modulus of 1.53 to 4.29 MPa, full hydrophilicity, favorable skin adhesivity, and biocompatibility. The simultaneous release was investigated in vitro indicating dependence on the scaffold’s composition and type of bioactive agents. The novel scaffolds designed as multi-target therapy have significant promise for improved wound healing in a beneficial and non-invasive manner.
PB  - MDPI
T2  - Pharmaceutics
T1  - Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform
IS  - 3
SP  - 372
VL  - 16
DO  - 10.3390/pharmaceutics16030372
ER  - 

@article{
author = "Babić Radić, Marija M. and Vukomanović, Marija and Nikodinović-Runić, Jasmina and Tomić, Simonida",
year = "2024",
abstract = "This study proposes synthesis and evaluation of gelatin-/alginate-based hydrogel scaffolds reinforced with titanium dioxide (TiO2) nanoparticles which, through their combination with allantoin, quercetin, and caffeic acid, provide multi-target therapy directed on all phases of the wound healing process. These scaffolds provide the simultaneous release of bioactive agents and concurrently support cell/tissue repair through the replicated structure of a native extracellular matrix. The hydrogel scaffolds were synthesized via a crosslinking reaction using EDC as a crosslinker for gelatin. Synthesized hydrogel scaffolds and the effect of TiO2 on their properties were characterized by structural, mechanical, morphological, and swelling properties, and the porosity, wettability, adhesion to skin tissue, and simultaneous release features. The biocompatibility of the scaffolds was tested in vitro on fibroblasts (MRC5 cells) and in vivo (Caenorhabditis elegans) in a survival probe. The scaffolds revealed porous interconnected morphology, porosity of 88.33 to 96.76%, elastic modulus of 1.53 to 4.29 MPa, full hydrophilicity, favorable skin adhesivity, and biocompatibility. The simultaneous release was investigated in vitro indicating dependence on the scaffold’s composition and type of bioactive agents. The novel scaffolds designed as multi-target therapy have significant promise for improved wound healing in a beneficial and non-invasive manner.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform",
number = "3",
pages = "372",
volume = "16",
doi = "10.3390/pharmaceutics16030372"
}

Babić Radić, M. M., Vukomanović, M., Nikodinović-Runić, J.,& Tomić, S.. (2024). Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform. in Pharmaceutics
MDPI., 16(3), 372.
https://doi.org/10.3390/pharmaceutics16030372

Babić Radić MM, Vukomanović M, Nikodinović-Runić J, Tomić S. Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform. in Pharmaceutics. 2024;16(3):372.
doi:10.3390/pharmaceutics16030372 .

Babić Radić, Marija M., Vukomanović, Marija, Nikodinović-Runić, Jasmina, Tomić, Simonida, "Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform" in Pharmaceutics, 16, no. 3 (2024):372,
https://doi.org/10.3390/pharmaceutics16030372 . .

TY  - JOUR
AU  - Tomić, Simonida Lj.
AU  - Vuković, Jovana S.
AU  - Babić Radić, Marija M.
AU  - Filipović, Vuk V.
AU  - Živanović, Dubravka P.
AU  - Nikolić, Miloš M.
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5869
AB  - Scaffolding biomaterials are gaining great importance due to their beneficial properties for medical purposes. Targeted biomaterial engineering strategies through the synergy of different material types can be applied to design hybrid scaffolding biomaterials with advantageous properties for biomedical applications. In our research, a novel combination of the bioactive agent Manuka honey (MHo) with 2-hydroxyethyl methacrylate/gelatin (HG) hydrogel scaffolds was created as an efficient bioactive platform for biomedical applications. The effects of Manuka honey content on structural characteristics, porosity, swelling performance, in vitro degradation, and in vitro biocompatibility (fibroblast and keratinocyte cell lines) of hybrid hydrogel scaffolds were studied using Fourier transform infrared spectroscopy, the gravimetric method, and in vitro MTT biocompatibility assays. The engineered hybrid hydrogel scaffolds show advantageous properties, including porosity in the range of 71.25% to 90.09%, specific pH- and temperature-dependent swelling performance, and convenient absorption capacity. In vitro degradation studies showed scaffold degradability ranging from 6.27% to 27.18% for four weeks. In vitro biocompatibility assays on healthy human fibroblast (MRC5 cells) and keratinocyte (HaCaT cells) cell lines by MTT tests showed that cell viability depends on the Manuka honey content loaded in the HG hydrogel scaffolds. A sample containing the highest Manuka honey content (30%) exhibited the best biocompatible properties. The obtained results reveal that the synergy of the bioactive agent, Manuka honey, with 2-hydroxyethyl methacrylate/gelatin as hybrid hydrogel scaffolds has potential for biomedical purposes. By tuning the Manuka honey content in HG hydrogel scaffolds advantageous properties of hybrid scaffolds can be achieved for biomedical applications.
PB  - MDPI
T2  - Polymers
T1  - Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration
IS  - 3
SP  - 589
VL  - 15
DO  - 10.3390/polym15030589
ER  - 

@article{
author = "Tomić, Simonida Lj. and Vuković, Jovana S. and Babić Radić, Marija M. and Filipović, Vuk V. and Živanović, Dubravka P. and Nikolić, Miloš M. and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Scaffolding biomaterials are gaining great importance due to their beneficial properties for medical purposes. Targeted biomaterial engineering strategies through the synergy of different material types can be applied to design hybrid scaffolding biomaterials with advantageous properties for biomedical applications. In our research, a novel combination of the bioactive agent Manuka honey (MHo) with 2-hydroxyethyl methacrylate/gelatin (HG) hydrogel scaffolds was created as an efficient bioactive platform for biomedical applications. The effects of Manuka honey content on structural characteristics, porosity, swelling performance, in vitro degradation, and in vitro biocompatibility (fibroblast and keratinocyte cell lines) of hybrid hydrogel scaffolds were studied using Fourier transform infrared spectroscopy, the gravimetric method, and in vitro MTT biocompatibility assays. The engineered hybrid hydrogel scaffolds show advantageous properties, including porosity in the range of 71.25% to 90.09%, specific pH- and temperature-dependent swelling performance, and convenient absorption capacity. In vitro degradation studies showed scaffold degradability ranging from 6.27% to 27.18% for four weeks. In vitro biocompatibility assays on healthy human fibroblast (MRC5 cells) and keratinocyte (HaCaT cells) cell lines by MTT tests showed that cell viability depends on the Manuka honey content loaded in the HG hydrogel scaffolds. A sample containing the highest Manuka honey content (30%) exhibited the best biocompatible properties. The obtained results reveal that the synergy of the bioactive agent, Manuka honey, with 2-hydroxyethyl methacrylate/gelatin as hybrid hydrogel scaffolds has potential for biomedical purposes. By tuning the Manuka honey content in HG hydrogel scaffolds advantageous properties of hybrid scaffolds can be achieved for biomedical applications.",
publisher = "MDPI",
journal = "Polymers",
title = "Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration",
number = "3",
pages = "589",
volume = "15",
doi = "10.3390/polym15030589"
}

Tomić, S. Lj., Vuković, J. S., Babić Radić, M. M., Filipović, V. V., Živanović, D. P., Nikolić, M. M.,& Nikodinović-Runić, J.. (2023). Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration. in Polymers
MDPI., 15(3), 589.
https://doi.org/10.3390/polym15030589

Tomić SL, Vuković JS, Babić Radić MM, Filipović VV, Živanović DP, Nikolić MM, Nikodinović-Runić J. Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration. in Polymers. 2023;15(3):589.
doi:10.3390/polym15030589 .

Tomić, Simonida Lj., Vuković, Jovana S., Babić Radić, Marija M., Filipović, Vuk V., Živanović, Dubravka P., Nikolić, Miloš M., Nikodinović-Runić, Jasmina, "Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration" in Polymers, 15, no. 3 (2023):589,
https://doi.org/10.3390/polym15030589 . .

TY  - JOUR
AU  - Babić Radić, Marija M.
AU  - Filipović, Vuk V.
AU  - Vuković, Jovana S.
AU  - Vukomanović, Marija
AU  - Ilić-Tomić, Tatjana
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida Lj.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6360
AB  - The idea of this study was to create a new scaffolding system based on 2-hydroxyethyl methacrylate, gelatin, and alginate that contains titanium(IV) oxide nanoparticles as a platform for the controlled release of the bioactive agent curcumin. The innovative strategy to develop hybrid scaffolds was the modified porogenation method. The effect of the scaffold composition on the chemical, morphology, porosity, mechanical, hydrophilicity, swelling, degradation, biocompatibility, loading, and release features of hybrid scaffolds was evaluated. A porous structure with interconnected pores in the range of 52.33–65.76%, favorable swelling capacity, fully hydrophilic surfaces, degradability to 45% for 6 months, curcumin loading efficiency above 96%, and favorable controlled release profiles were obtained. By applying four kinetic models of release, valuable parameters were obtained for the curcumin/PHEMA/gelatin/alginate/TiO2 release platform. Cytotoxicity test results depend on the composition of the scaffolds and showed satisfactory cell growth with visible cell accumulation on the hybrid surfaces. The constructed hybrid scaffolds have suitable high-performance properties, suggesting potential for further in vivo and clinical studies.
PB  - MDPI
T2  - Polymers
T1  - 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Scaffolds Reinforced with Nano TiO2 as a Promising Curcumin Release Platform
IS  - 7
SP  - 1643
VL  - 15
DO  - 10.3390/polym15071643
ER  - 

@article{
author = "Babić Radić, Marija M. and Filipović, Vuk V. and Vuković, Jovana S. and Vukomanović, Marija and Ilić-Tomić, Tatjana and Nikodinović-Runić, Jasmina and Tomić, Simonida Lj.",
year = "2023",
abstract = "The idea of this study was to create a new scaffolding system based on 2-hydroxyethyl methacrylate, gelatin, and alginate that contains titanium(IV) oxide nanoparticles as a platform for the controlled release of the bioactive agent curcumin. The innovative strategy to develop hybrid scaffolds was the modified porogenation method. The effect of the scaffold composition on the chemical, morphology, porosity, mechanical, hydrophilicity, swelling, degradation, biocompatibility, loading, and release features of hybrid scaffolds was evaluated. A porous structure with interconnected pores in the range of 52.33–65.76%, favorable swelling capacity, fully hydrophilic surfaces, degradability to 45% for 6 months, curcumin loading efficiency above 96%, and favorable controlled release profiles were obtained. By applying four kinetic models of release, valuable parameters were obtained for the curcumin/PHEMA/gelatin/alginate/TiO2 release platform. Cytotoxicity test results depend on the composition of the scaffolds and showed satisfactory cell growth with visible cell accumulation on the hybrid surfaces. The constructed hybrid scaffolds have suitable high-performance properties, suggesting potential for further in vivo and clinical studies.",
publisher = "MDPI",
journal = "Polymers",
title = "2-Hydroxyethyl Methacrylate/Gelatin/Alginate Scaffolds Reinforced with Nano TiO2 as a Promising Curcumin Release Platform",
number = "7",
pages = "1643",
volume = "15",
doi = "10.3390/polym15071643"
}

Babić Radić, M. M., Filipović, V. V., Vuković, J. S., Vukomanović, M., Ilić-Tomić, T., Nikodinović-Runić, J.,& Tomić, S. Lj.. (2023). 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Scaffolds Reinforced with Nano TiO2 as a Promising Curcumin Release Platform. in Polymers
MDPI., 15(7), 1643.
https://doi.org/10.3390/polym15071643

Babić Radić MM, Filipović VV, Vuković JS, Vukomanović M, Ilić-Tomić T, Nikodinović-Runić J, Tomić SL. 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Scaffolds Reinforced with Nano TiO2 as a Promising Curcumin Release Platform. in Polymers. 2023;15(7):1643.
doi:10.3390/polym15071643 .

Babić Radić, Marija M., Filipović, Vuk V., Vuković, Jovana S., Vukomanović, Marija, Ilić-Tomić, Tatjana, Nikodinović-Runić, Jasmina, Tomić, Simonida Lj., "2-Hydroxyethyl Methacrylate/Gelatin/Alginate Scaffolds Reinforced with Nano TiO2 as a Promising Curcumin Release Platform" in Polymers, 15, no. 7 (2023):1643,
https://doi.org/10.3390/polym15071643 . .

TY  - JOUR
AU  - Tomić, Simonida Lj.
AU  - Babić Radić, Marija M.
AU  - Vuković, Jovana S.
AU  - Filipović, Vuk V.
AU  - Nikodinović-Runić, Jasmina
AU  - Vukomanović, Marija
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6356
AB  - Alginate is a natural polymer of marine origin and, due to its exceptional properties, has great importance as an essential component for the preparation of hydrogels and scaffolds for biomedical applications. The design of biologically interactive hydrogels and scaffolds with advanced, expected and required properties are one of the key issues for successful outcomes in the healing of injured tissues. This review paper presents the multifunctional biomedical applications of alginate-based hydrogels and scaffolds in selected areas, highlighting the key effect of alginate and its influence on the essential properties of the selected biomedical applications. The first part covers scientific achievements for alginate in dermal tissue regeneration, drug delivery systems, cancer treatment, and antimicrobials. The second part is dedicated to our scientific results obtained for the research opus of hydrogel materials for scaffolds based on alginate in synergy with different materials (polymers and bioactive agents). Alginate has proved to be an exceptional polymer for combining with other naturally occurring and synthetic polymers, as well as loading bioactive therapeutic agents to achieve dermal, controlled drug delivery, cancer treatment, and antimicrobial purposes. Our research was based on combinations of alginate with gelatin, 2-hydroxyethyl methacrylate, apatite, graphene oxide and iron(III) oxide, as well as curcumin and resveratrol as bioactive agents. Important features of the prepared scaffolds, such as morphology, porosity, absorption capacity, hydrophilicity, mechanical properties, in vitro degradation, and in vitro and in vivo biocompatibility, have shown favorable properties for the aforementioned applications, and alginate has been an important link in achieving these properties. Alginate, as a component of these systems, proved to be an indispensable factor and played an excellent “role” in the optimal adjustment of the tested properties. This study provides valuable data and information for researchers and demonstrates the importance of the role of alginate as a biomaterial in the design of hydrogels and scaffolds that are powerful medical “tools” for biomedical applications.
PB  - MDPI
T2  - Marine Drugs
T1  - Alginate-Based Hydrogels and Scaffolds for Biomedical Applications
IS  - 3
SP  - 177
VL  - 21
DO  - 10.3390/md21030177
ER  - 

@article{
author = "Tomić, Simonida Lj. and Babić Radić, Marija M. and Vuković, Jovana S. and Filipović, Vuk V. and Nikodinović-Runić, Jasmina and Vukomanović, Marija",
year = "2023",
abstract = "Alginate is a natural polymer of marine origin and, due to its exceptional properties, has great importance as an essential component for the preparation of hydrogels and scaffolds for biomedical applications. The design of biologically interactive hydrogels and scaffolds with advanced, expected and required properties are one of the key issues for successful outcomes in the healing of injured tissues. This review paper presents the multifunctional biomedical applications of alginate-based hydrogels and scaffolds in selected areas, highlighting the key effect of alginate and its influence on the essential properties of the selected biomedical applications. The first part covers scientific achievements for alginate in dermal tissue regeneration, drug delivery systems, cancer treatment, and antimicrobials. The second part is dedicated to our scientific results obtained for the research opus of hydrogel materials for scaffolds based on alginate in synergy with different materials (polymers and bioactive agents). Alginate has proved to be an exceptional polymer for combining with other naturally occurring and synthetic polymers, as well as loading bioactive therapeutic agents to achieve dermal, controlled drug delivery, cancer treatment, and antimicrobial purposes. Our research was based on combinations of alginate with gelatin, 2-hydroxyethyl methacrylate, apatite, graphene oxide and iron(III) oxide, as well as curcumin and resveratrol as bioactive agents. Important features of the prepared scaffolds, such as morphology, porosity, absorption capacity, hydrophilicity, mechanical properties, in vitro degradation, and in vitro and in vivo biocompatibility, have shown favorable properties for the aforementioned applications, and alginate has been an important link in achieving these properties. Alginate, as a component of these systems, proved to be an indispensable factor and played an excellent “role” in the optimal adjustment of the tested properties. This study provides valuable data and information for researchers and demonstrates the importance of the role of alginate as a biomaterial in the design of hydrogels and scaffolds that are powerful medical “tools” for biomedical applications.",
publisher = "MDPI",
journal = "Marine Drugs",
title = "Alginate-Based Hydrogels and Scaffolds for Biomedical Applications",
number = "3",
pages = "177",
volume = "21",
doi = "10.3390/md21030177"
}

Tomić, S. Lj., Babić Radić, M. M., Vuković, J. S., Filipović, V. V., Nikodinović-Runić, J.,& Vukomanović, M.. (2023). Alginate-Based Hydrogels and Scaffolds for Biomedical Applications. in Marine Drugs
MDPI., 21(3), 177.
https://doi.org/10.3390/md21030177

Tomić SL, Babić Radić MM, Vuković JS, Filipović VV, Nikodinović-Runić J, Vukomanović M. Alginate-Based Hydrogels and Scaffolds for Biomedical Applications. in Marine Drugs. 2023;21(3):177.
doi:10.3390/md21030177 .

Tomić, Simonida Lj., Babić Radić, Marija M., Vuković, Jovana S., Filipović, Vuk V., Nikodinović-Runić, Jasmina, Vukomanović, Marija, "Alginate-Based Hydrogels and Scaffolds for Biomedical Applications" in Marine Drugs, 21, no. 3 (2023):177,
https://doi.org/10.3390/md21030177 . .

TY  - JOUR
AU  - Filipović, Vuk V.
AU  - Babić Radić, Marija M.
AU  - Vuković, Jovana S.
AU  - Vukomanović, Marija
AU  - Rubert, Marina
AU  - Hofmann, Sandra
AU  - Müller, Ralph
AU  - Tomić, Simonida Lj.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5018
AB  - New composite 3D scaffolds were developed as a combination of synthetic polymer, poly(2-hydroxyethyl methacrylate) (PHEMA), and a natural polymer, gelatin, with a ceramic component, nanohydroxyapatite (ID nHAp) dopped with metal ions. The combination of a synthetic polymer, to be able to tune the structure and the physicochemical and mechanical properties, and a natural polymer, to ensure the specific biological functions of the scaffold, with inorganic filler was applied. The goal was to make a new material with superior properties for applications in the biomedical field which mimics as closely as possible the native bone extracellular matrix (ECM). Biodegradable PHEMA hydrogel was obtained by crosslinking HEMA by poly(beta-amino esters) (PBAE). The scaffold's physicochemical and mechanical properties, in vitro degradation, and biological activity were assessed so to study the effects of the incorporation of nHAp in the (PHEMA/PBAE/gelatin) hydrogel, as well as the effect of the different pore-forming methods. Cryogels had higher elasticity, swelling, porosity, and percent of mass loss during degradation than the samples obtained by porogenation. The composite scaffolds had a higher mechanical strength, 10.14 MPa for the porogenated samples and 5.87 MPa for the cryogels, but a slightly lower degree of swelling, percent of mass loss, and porosity than the hybrid ones. All the scaffolds were nontoxic and had a high cell adhesion rate, which was 15-20% higher in the composite samples. Cell metabolic activity after 2 and 7 days of culture was higher in the composites, although not statistically different. After 28 days, cell metabolic activity was similar in all scaffolds and the TCP control. No effect of integrating nHAp into the scaffolds on osteogenic cell differentiation could be observed. Synergetic effects occurred which influenced the mechanical behavior, structure, physicochemical properties, and interactions with biological species.
T2  - Polymers
T1  - Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(beta-amino esters), and Hydroxyapatite
IS  - 1
SP  - 18
VL  - 14
DO  - 10.3390/polym14010018
ER  - 

@article{
author = "Filipović, Vuk V. and Babić Radić, Marija M. and Vuković, Jovana S. and Vukomanović, Marija and Rubert, Marina and Hofmann, Sandra and Müller, Ralph and Tomić, Simonida Lj.",
year = "2022",
abstract = "New composite 3D scaffolds were developed as a combination of synthetic polymer, poly(2-hydroxyethyl methacrylate) (PHEMA), and a natural polymer, gelatin, with a ceramic component, nanohydroxyapatite (ID nHAp) dopped with metal ions. The combination of a synthetic polymer, to be able to tune the structure and the physicochemical and mechanical properties, and a natural polymer, to ensure the specific biological functions of the scaffold, with inorganic filler was applied. The goal was to make a new material with superior properties for applications in the biomedical field which mimics as closely as possible the native bone extracellular matrix (ECM). Biodegradable PHEMA hydrogel was obtained by crosslinking HEMA by poly(beta-amino esters) (PBAE). The scaffold's physicochemical and mechanical properties, in vitro degradation, and biological activity were assessed so to study the effects of the incorporation of nHAp in the (PHEMA/PBAE/gelatin) hydrogel, as well as the effect of the different pore-forming methods. Cryogels had higher elasticity, swelling, porosity, and percent of mass loss during degradation than the samples obtained by porogenation. The composite scaffolds had a higher mechanical strength, 10.14 MPa for the porogenated samples and 5.87 MPa for the cryogels, but a slightly lower degree of swelling, percent of mass loss, and porosity than the hybrid ones. All the scaffolds were nontoxic and had a high cell adhesion rate, which was 15-20% higher in the composite samples. Cell metabolic activity after 2 and 7 days of culture was higher in the composites, although not statistically different. After 28 days, cell metabolic activity was similar in all scaffolds and the TCP control. No effect of integrating nHAp into the scaffolds on osteogenic cell differentiation could be observed. Synergetic effects occurred which influenced the mechanical behavior, structure, physicochemical properties, and interactions with biological species.",
journal = "Polymers",
title = "Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(beta-amino esters), and Hydroxyapatite",
number = "1",
pages = "18",
volume = "14",
doi = "10.3390/polym14010018"
}

Filipović, V. V., Babić Radić, M. M., Vuković, J. S., Vukomanović, M., Rubert, M., Hofmann, S., Müller, R.,& Tomić, S. Lj.. (2022). Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(beta-amino esters), and Hydroxyapatite. in Polymers, 14(1), 18.
https://doi.org/10.3390/polym14010018

Filipović VV, Babić Radić MM, Vuković JS, Vukomanović M, Rubert M, Hofmann S, Müller R, Tomić SL. Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(beta-amino esters), and Hydroxyapatite. in Polymers. 2022;14(1):18.
doi:10.3390/polym14010018 .

Filipović, Vuk V., Babić Radić, Marija M., Vuković, Jovana S., Vukomanović, Marija, Rubert, Marina, Hofmann, Sandra, Müller, Ralph, Tomić, Simonida Lj., "Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(beta-amino esters), and Hydroxyapatite" in Polymers, 14, no. 1 (2022):18,
https://doi.org/10.3390/polym14010018 . .

TY  - JOUR
AU  - Babić Radić, Marija M.
AU  - Filipovic, Vuk V.
AU  - Vukomanovic, Marija
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5023
AB  - The design and evaluation of novel 2-hydroxyethyl methacrylate/gelatin/alginate/graphene oxide hydrogels as innovative scaffolding biomaterials, which concurrently are the suitable drug delivery carrier, was proposed. The hydrogels were prepared by the adapted porogen leaching method; this is also the first time this method has been used to incorporate nanocolloidal graphene oxide through the hydrogel and simultaneously form porous structures. The effects of a material's composition on its chemical, morphological, mechanical, and swelling properties, as well as on cell viability and in vitro degradation, were assessed using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), measurements of Young's modulus, gravimetric method and MTT test, respectively. The engineered hydrogels show good swelling capacity, fully hydrophilic surfaces, tunable porosity (from 56 to 76%) and mechanical properties (from 1.69 to 4.78 MPa), curcumin entrapment efficiency above 99% and excellent curcumin release performances. In vitro cytotoxicity on healthy human fibroblast (MRC5 cells) by MTT test reveal that the materials are nontoxic and biocompatible, proposing novel hydrogels for in vivo clinical evaluation to optimize tissue regeneration treatments by coupling the hydrogels with cells and different active agents to create material/biofactor hybrids with new levels of biofunctionality.
T2  - Gels
T1  - Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials
IS  - 1
SP  - 22
VL  - 8
DO  - 10.3390/gels8010022
ER  - 

@article{
author = "Babić Radić, Marija M. and Filipovic, Vuk V. and Vukomanovic, Marija and Nikodinović-Runić, Jasmina and Tomić, Simonida",
year = "2022",
abstract = "The design and evaluation of novel 2-hydroxyethyl methacrylate/gelatin/alginate/graphene oxide hydrogels as innovative scaffolding biomaterials, which concurrently are the suitable drug delivery carrier, was proposed. The hydrogels were prepared by the adapted porogen leaching method; this is also the first time this method has been used to incorporate nanocolloidal graphene oxide through the hydrogel and simultaneously form porous structures. The effects of a material's composition on its chemical, morphological, mechanical, and swelling properties, as well as on cell viability and in vitro degradation, were assessed using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), measurements of Young's modulus, gravimetric method and MTT test, respectively. The engineered hydrogels show good swelling capacity, fully hydrophilic surfaces, tunable porosity (from 56 to 76%) and mechanical properties (from 1.69 to 4.78 MPa), curcumin entrapment efficiency above 99% and excellent curcumin release performances. In vitro cytotoxicity on healthy human fibroblast (MRC5 cells) by MTT test reveal that the materials are nontoxic and biocompatible, proposing novel hydrogels for in vivo clinical evaluation to optimize tissue regeneration treatments by coupling the hydrogels with cells and different active agents to create material/biofactor hybrids with new levels of biofunctionality.",
journal = "Gels",
title = "Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials",
number = "1",
pages = "22",
volume = "8",
doi = "10.3390/gels8010022"
}

Babić Radić, M. M., Filipovic, V. V., Vukomanovic, M., Nikodinović-Runić, J.,& Tomić, S.. (2022). Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials. in Gels, 8(1), 22.
https://doi.org/10.3390/gels8010022

Babić Radić MM, Filipovic VV, Vukomanovic M, Nikodinović-Runić J, Tomić S. Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials. in Gels. 2022;8(1):22.
doi:10.3390/gels8010022 .

Babić Radić, Marija M., Filipovic, Vuk V., Vukomanovic, Marija, Nikodinović-Runić, Jasmina, Tomić, Simonida, "Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials" in Gels, 8, no. 1 (2022):22,
https://doi.org/10.3390/gels8010022 . .

TY  - JOUR
AU  - Tomić, Simonida Lj.
AU  - Vuković, Jovana S.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5065
AB  - The design and use of new potent and specific antimicrobial systems are of crucial importance in the medical field. This will help relieve, fight, and eradicate infections and thus improve
human health. The use of metals in various forms as antimicrobial therapeutics has been known since
ancient times. In this sense, polymeric hydrogel matrices as multifunctional materials and in combination with various metal forms can be a great alternative to conventional treatments for infections.
Hydrogels possess high hydrophilicity, specific three-dimensional networks, fine biocompatibility,
and cell adhesion and are therefore suitable as materials for the loading of active antimicrobial agents
and acting in antimicrobial areas. The biocompatible nature of hydrogels’ matrices makes them a
convenient starting platform to develop biocompatible, selective, active controlled-release antimicrobial materials. Hydrogels based on acrylate and itaconic acid were synthesized and loaded with
silver (Ag+
), copper (Cu2+), and zinc (Zn2+) ions as a controlled release and antimicrobial system to
test release properties and antimicrobial activity in contact with microbes. The metal ions/hydrogel
systems exhibited favorable biocompatibility, release profiles, and antimicrobial activity against
methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA),
Staphylococcus aureus, Escherichia coli, and Candida albicans microbes, and have shown that they have
the capacity to “fight” with the life-threatening infections. Antimicrobial activity depends on types
of metal ions, the composition of polymeric matrices, as well as the types of microbes. Designed
metal ions/poly(acrylate/itaconic acid) antimicrobial systems have shown to have good potential as
antimicrobial therapeutics and suitable biomaterials for medical applications.
PB  - MDPI
T2  - Inorganics
T1  - Antimicrobial Activity of Silver, Copper, and Zinc Ions/Poly(Acrylate/Itaconic Acid) Hydrogel Matrices
IS  - 3
SP  - 38
VL  - 10
DO  - 10.3390/inorganics10030038
ER  - 

@article{
author = "Tomić, Simonida Lj. and Vuković, Jovana S.",
year = "2022",
abstract = "The design and use of new potent and specific antimicrobial systems are of crucial importance in the medical field. This will help relieve, fight, and eradicate infections and thus improve
human health. The use of metals in various forms as antimicrobial therapeutics has been known since
ancient times. In this sense, polymeric hydrogel matrices as multifunctional materials and in combination with various metal forms can be a great alternative to conventional treatments for infections.
Hydrogels possess high hydrophilicity, specific three-dimensional networks, fine biocompatibility,
and cell adhesion and are therefore suitable as materials for the loading of active antimicrobial agents
and acting in antimicrobial areas. The biocompatible nature of hydrogels’ matrices makes them a
convenient starting platform to develop biocompatible, selective, active controlled-release antimicrobial materials. Hydrogels based on acrylate and itaconic acid were synthesized and loaded with
silver (Ag+
), copper (Cu2+), and zinc (Zn2+) ions as a controlled release and antimicrobial system to
test release properties and antimicrobial activity in contact with microbes. The metal ions/hydrogel
systems exhibited favorable biocompatibility, release profiles, and antimicrobial activity against
methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA),
Staphylococcus aureus, Escherichia coli, and Candida albicans microbes, and have shown that they have
the capacity to “fight” with the life-threatening infections. Antimicrobial activity depends on types
of metal ions, the composition of polymeric matrices, as well as the types of microbes. Designed
metal ions/poly(acrylate/itaconic acid) antimicrobial systems have shown to have good potential as
antimicrobial therapeutics and suitable biomaterials for medical applications.",
publisher = "MDPI",
journal = "Inorganics",
title = "Antimicrobial Activity of Silver, Copper, and Zinc Ions/Poly(Acrylate/Itaconic Acid) Hydrogel Matrices",
number = "3",
pages = "38",
volume = "10",
doi = "10.3390/inorganics10030038"
}

Tomić, S. Lj.,& Vuković, J. S.. (2022). Antimicrobial Activity of Silver, Copper, and Zinc Ions/Poly(Acrylate/Itaconic Acid) Hydrogel Matrices. in Inorganics
MDPI., 10(3), 38.
https://doi.org/10.3390/inorganics10030038

Tomić SL, Vuković JS. Antimicrobial Activity of Silver, Copper, and Zinc Ions/Poly(Acrylate/Itaconic Acid) Hydrogel Matrices. in Inorganics. 2022;10(3):38.
doi:10.3390/inorganics10030038 .

Tomić, Simonida Lj., Vuković, Jovana S., "Antimicrobial Activity of Silver, Copper, and Zinc Ions/Poly(Acrylate/Itaconic Acid) Hydrogel Matrices" in Inorganics, 10, no. 3 (2022):38,
https://doi.org/10.3390/inorganics10030038 . .

TY  - JOUR
AU  - Babić Radić, Marija M.
AU  - Filipović, Vuk V.
AU  - Vuković, Jovana S.
AU  - Vukomanović, Marija
AU  - Rubert, Marina
AU  - Hofmann, Sandra
AU  - Müller, Ralph
AU  - Tomić, Simonida Lj.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5201
AB  - Our goal was to create bioimitated scaffolding materials for biomedical purposes. The guiding idea was that we used an interpenetrating structural hierarchy of natural extracellular matrix as a “pattern” to design hydrogel scaffolds that show favorable properties for tissue regeneration. Polymeric hydrogel scaffolds are made in a simple, environmentally friendly way without additional functionalization. Gelatin and 2-hydroxyethyl methacrylate were selected to prepare interpenetrating polymeric networks and linear alginate chains were added as an interpenetrant to study their influence on the scaffold’s functionalities. Cryogelation and porogenation methods were used to obtain the designed scaffolding biomaterials. The scaffold’s structural, morphological, and mechanical properties, in vitro degradation, and cell viability properties were assessed to study the effects of the preparation method and alginate loading. Apatite as an inorganic agent was incorporated into cryogelated scaffolds to perform an extensive biological assay. Cryogelated scaffolds possess superior functionalities essential for tissue regeneration: fully hydrophilicity, degradability and mechanical features (2.08–9.75 MPa), and an optimal LDH activity. Furthermore, cryogelated scaffolds loaded with apatite showed good cell adhesion capacity, biocompatibility, and non-toxic behavior. All scaffolds performed equally in terms of metabolic activity and osteoconductivity. Cryogelated scaffolds with/without HAp could represent a new advance to promote osteoconductivity and enhance hard tissue repair. The obtained series of scaffolding biomaterials described here can provide a wide range of potential applications in the area of biomedical engineering.
PB  - MDPI
T2  - Polymers
T1  - Bioactive Interpenetrating Hydrogel Networks Based on 2-Hydroxyethyl Methacrylate and Gelatin Intertwined with Alginate and Dopped with Apatite as Scaffolding Biomaterials
IS  - 15
SP  - 3112
VL  - 14
DO  - 10.3390/polym14153112
ER  - 

@article{
author = "Babić Radić, Marija M. and Filipović, Vuk V. and Vuković, Jovana S. and Vukomanović, Marija and Rubert, Marina and Hofmann, Sandra and Müller, Ralph and Tomić, Simonida Lj.",
year = "2022",
abstract = "Our goal was to create bioimitated scaffolding materials for biomedical purposes. The guiding idea was that we used an interpenetrating structural hierarchy of natural extracellular matrix as a “pattern” to design hydrogel scaffolds that show favorable properties for tissue regeneration. Polymeric hydrogel scaffolds are made in a simple, environmentally friendly way without additional functionalization. Gelatin and 2-hydroxyethyl methacrylate were selected to prepare interpenetrating polymeric networks and linear alginate chains were added as an interpenetrant to study their influence on the scaffold’s functionalities. Cryogelation and porogenation methods were used to obtain the designed scaffolding biomaterials. The scaffold’s structural, morphological, and mechanical properties, in vitro degradation, and cell viability properties were assessed to study the effects of the preparation method and alginate loading. Apatite as an inorganic agent was incorporated into cryogelated scaffolds to perform an extensive biological assay. Cryogelated scaffolds possess superior functionalities essential for tissue regeneration: fully hydrophilicity, degradability and mechanical features (2.08–9.75 MPa), and an optimal LDH activity. Furthermore, cryogelated scaffolds loaded with apatite showed good cell adhesion capacity, biocompatibility, and non-toxic behavior. All scaffolds performed equally in terms of metabolic activity and osteoconductivity. Cryogelated scaffolds with/without HAp could represent a new advance to promote osteoconductivity and enhance hard tissue repair. The obtained series of scaffolding biomaterials described here can provide a wide range of potential applications in the area of biomedical engineering.",
publisher = "MDPI",
journal = "Polymers",
title = "Bioactive Interpenetrating Hydrogel Networks Based on 2-Hydroxyethyl Methacrylate and Gelatin Intertwined with Alginate and Dopped with Apatite as Scaffolding Biomaterials",
number = "15",
pages = "3112",
volume = "14",
doi = "10.3390/polym14153112"
}

Babić Radić, M. M., Filipović, V. V., Vuković, J. S., Vukomanović, M., Rubert, M., Hofmann, S., Müller, R.,& Tomić, S. Lj.. (2022). Bioactive Interpenetrating Hydrogel Networks Based on 2-Hydroxyethyl Methacrylate and Gelatin Intertwined with Alginate and Dopped with Apatite as Scaffolding Biomaterials. in Polymers
MDPI., 14(15), 3112.
https://doi.org/10.3390/polym14153112

Babić Radić MM, Filipović VV, Vuković JS, Vukomanović M, Rubert M, Hofmann S, Müller R, Tomić SL. Bioactive Interpenetrating Hydrogel Networks Based on 2-Hydroxyethyl Methacrylate and Gelatin Intertwined with Alginate and Dopped with Apatite as Scaffolding Biomaterials. in Polymers. 2022;14(15):3112.
doi:10.3390/polym14153112 .

Babić Radić, Marija M., Filipović, Vuk V., Vuković, Jovana S., Vukomanović, Marija, Rubert, Marina, Hofmann, Sandra, Müller, Ralph, Tomić, Simonida Lj., "Bioactive Interpenetrating Hydrogel Networks Based on 2-Hydroxyethyl Methacrylate and Gelatin Intertwined with Alginate and Dopped with Apatite as Scaffolding Biomaterials" in Polymers, 14, no. 15 (2022):3112,
https://doi.org/10.3390/polym14153112 . .

TY  - JOUR
AU  - Vuković, Jovana S.
AU  - Filipović, Vuk V.
AU  - Babić Radić, Marija M.
AU  - Vukomanović, Marija
AU  - Milivojević, Dušan
AU  - Ilić-Tomić, Tatjana
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida Lj.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5242
AB  - Scaffold hydrogel biomaterials designed to have advantageous biofunctional properties, which can be applied for controlled bioactive agent release, represent an important concept in biomedical tissue engineering. Our goal was to create scaffolding materials that mimic living tissue for biomedical utilization. In this study, two novel series of interpenetrating hydrogel networks (IPNs) based on 2-hydroxyethyl methacrylate/gelatin and 2-hydroxyethyl methacrylate/alginate were crosslinked using N-ethyl-N′-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Characterization included examining the effects of crosslinker type and concentration on structure, morphological and mechanical properties, in vitro swelling, hydrophilicity as well as on the in vitro cell viability (fibroblast cells) and in vivo (Caenorhabditis elegans) interactions of novel biomaterials. The engineered IPN hydrogel scaffolds show an interconnected pore morphology and porosity range of 62.36 to 85.20%, favorable in vitro swelling capacity, full hydrophilicity, and Young’s modulus values in the range of 1.40 to 7.50 MPa. In vitro assay on healthy human fibroblast (MRC5 cells) by MTT test and in vivo (Caenorhabditis elegans) survival assays show the advantageous biocompatible properties of novel IPN hydrogel scaffolds. Furthermore, in vitro controlled release study of the therapeutic agent resveratrol showed that these novel scaffolding systems are suitable controlled release platforms. The results revealed that the use of EDC and the combination of EDC/NHS crosslinkers can be applied to prepare and tune the properties of the IPN 2-hydroxyethyl methacrylate/alginate and 2-hydroxyethyl methacrylate/gelatin hydrogel scaffolds series, which have shown great potential for biomedical engineering applications.
PB  - MDPI
T2  - Polymers
T1  - In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds
IS  - 20
SP  - 4459
VL  - 14
DO  - 10.3390/polym14204459
ER  - 

@article{
author = "Vuković, Jovana S. and Filipović, Vuk V. and Babić Radić, Marija M. and Vukomanović, Marija and Milivojević, Dušan and Ilić-Tomić, Tatjana and Nikodinović-Runić, Jasmina and Tomić, Simonida Lj.",
year = "2022",
abstract = "Scaffold hydrogel biomaterials designed to have advantageous biofunctional properties, which can be applied for controlled bioactive agent release, represent an important concept in biomedical tissue engineering. Our goal was to create scaffolding materials that mimic living tissue for biomedical utilization. In this study, two novel series of interpenetrating hydrogel networks (IPNs) based on 2-hydroxyethyl methacrylate/gelatin and 2-hydroxyethyl methacrylate/alginate were crosslinked using N-ethyl-N′-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Characterization included examining the effects of crosslinker type and concentration on structure, morphological and mechanical properties, in vitro swelling, hydrophilicity as well as on the in vitro cell viability (fibroblast cells) and in vivo (Caenorhabditis elegans) interactions of novel biomaterials. The engineered IPN hydrogel scaffolds show an interconnected pore morphology and porosity range of 62.36 to 85.20%, favorable in vitro swelling capacity, full hydrophilicity, and Young’s modulus values in the range of 1.40 to 7.50 MPa. In vitro assay on healthy human fibroblast (MRC5 cells) by MTT test and in vivo (Caenorhabditis elegans) survival assays show the advantageous biocompatible properties of novel IPN hydrogel scaffolds. Furthermore, in vitro controlled release study of the therapeutic agent resveratrol showed that these novel scaffolding systems are suitable controlled release platforms. The results revealed that the use of EDC and the combination of EDC/NHS crosslinkers can be applied to prepare and tune the properties of the IPN 2-hydroxyethyl methacrylate/alginate and 2-hydroxyethyl methacrylate/gelatin hydrogel scaffolds series, which have shown great potential for biomedical engineering applications.",
publisher = "MDPI",
journal = "Polymers",
title = "In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds",
number = "20",
pages = "4459",
volume = "14",
doi = "10.3390/polym14204459"
}

Vuković, J. S., Filipović, V. V., Babić Radić, M. M., Vukomanović, M., Milivojević, D., Ilić-Tomić, T., Nikodinović-Runić, J.,& Tomić, S. Lj.. (2022). In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds. in Polymers
MDPI., 14(20), 4459.
https://doi.org/10.3390/polym14204459

Vuković JS, Filipović VV, Babić Radić MM, Vukomanović M, Milivojević D, Ilić-Tomić T, Nikodinović-Runić J, Tomić SL. In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds. in Polymers. 2022;14(20):4459.
doi:10.3390/polym14204459 .

Vuković, Jovana S., Filipović, Vuk V., Babić Radić, Marija M., Vukomanović, Marija, Milivojević, Dušan, Ilić-Tomić, Tatjana, Nikodinović-Runić, Jasmina, Tomić, Simonida Lj., "In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds" in Polymers, 14, no. 20 (2022):4459,
https://doi.org/10.3390/polym14204459 . .

TY  - JOUR
AU  - Tomić, Simonida
AU  - Nikodinović-Runić, Jasmina
AU  - Vukomanovic, Marija
AU  - Babić, Marija M.
AU  - Vuković, Jovana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4926
AB  - Hydrogel scaffolding biomaterials are one of the most attractive polymeric biomaterials for regenerative engineering and can be engineered into tissue mimetic scaffolds to support cell growth due to their similarity to the native extracellular matrix. The novel, versatile hydrogel scaffolds based on alginate, gelatin, 2-hydroxyethyl methacrylate, and inorganic agent hydroxyapatite were prepared by modified cryogelation. The chemical composition, morphology, porosity, mechanical properties, effects on cell viability, in vitro degradation, in vitro and in vivo biocompatibility were tested to correlate the material's composition with the corresponding properties. Scaffolds showed an interconnected porous microstructure, satisfactory mechanical strength, favorable hydrophilicity, degradation, and suitable in vitro and in vivo biocompatible behavior. Materials showed good biocompatibility with healthy human fibroblast in cell culture, as well as in vivo with zebrafish assay, suggesting newly synthesized hydrogel scaffolds as a potential new generation of hydrogel scaffolding biomaterials with tunable properties for versatile biomedical applications and tissue regeneration.
T2  - Polymers
T1  - Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite
IS  - 6
SP  - 932
VL  - 13
DO  - 10.3390/polym13060932
ER  - 

@article{
author = "Tomić, Simonida and Nikodinović-Runić, Jasmina and Vukomanovic, Marija and Babić, Marija M. and Vuković, Jovana",
year = "2021",
abstract = "Hydrogel scaffolding biomaterials are one of the most attractive polymeric biomaterials for regenerative engineering and can be engineered into tissue mimetic scaffolds to support cell growth due to their similarity to the native extracellular matrix. The novel, versatile hydrogel scaffolds based on alginate, gelatin, 2-hydroxyethyl methacrylate, and inorganic agent hydroxyapatite were prepared by modified cryogelation. The chemical composition, morphology, porosity, mechanical properties, effects on cell viability, in vitro degradation, in vitro and in vivo biocompatibility were tested to correlate the material's composition with the corresponding properties. Scaffolds showed an interconnected porous microstructure, satisfactory mechanical strength, favorable hydrophilicity, degradation, and suitable in vitro and in vivo biocompatible behavior. Materials showed good biocompatibility with healthy human fibroblast in cell culture, as well as in vivo with zebrafish assay, suggesting newly synthesized hydrogel scaffolds as a potential new generation of hydrogel scaffolding biomaterials with tunable properties for versatile biomedical applications and tissue regeneration.",
journal = "Polymers",
title = "Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite",
number = "6",
pages = "932",
volume = "13",
doi = "10.3390/polym13060932"
}

Tomić, S., Nikodinović-Runić, J., Vukomanovic, M., Babić, M. M.,& Vuković, J.. (2021). Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite. in Polymers, 13(6), 932.
https://doi.org/10.3390/polym13060932

Tomić S, Nikodinović-Runić J, Vukomanovic M, Babić MM, Vuković J. Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite. in Polymers. 2021;13(6):932.
doi:10.3390/polym13060932 .

Tomić, Simonida, Nikodinović-Runić, Jasmina, Vukomanovic, Marija, Babić, Marija M., Vuković, Jovana, "Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite" in Polymers, 13, no. 6 (2021):932,
https://doi.org/10.3390/polym13060932 . .

TY  - JOUR
AU  - Antić, Katarina
AU  - Onjia, Antonije
AU  - Vasiljevic-Radovic, Dana
AU  - Veličković, Zlate
AU  - Tomić, Simonida
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4784
AB  - The adsorption of Ni2+ ions from water solutions by using hydrogels based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA) was studied. Hydrogel synthesis was optimized with response surface methodology (RSM). The hydrogel with the best adsorption capacity towards Ni2+ ions was chosen for further experiments. The hydrogel was characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM) analysis before and after the adsorption of Ni2+ ions. Batch equilibrium experiments were conducted to investigate the influence of solution pH, hydrogel weight, ionic strength, adsorption time, temperature and initial concentration of nickel ions on the adsorption. Time-dependent adsorption fitted the best to the pseudo-second-order kinetic model. A thermodynamic study revealed that the adsorption was an exothermic and non-spontaneous process. Five isotherm models were studied, and the best fit was obtained with the Redlich-Peterson model. Consecutive adsorption/desorption studies indicated that the HEA/IA hydrogel can be efficiently used as a sorbent for the removal of Ni2+ ions from the water solution. This study develops a potential adsorbent for the effective removal of trace nickel ions.
T2  - Gels
T1  - Removal of Nickel Ions from Aqueous Solutions by 2-Hydroxyethyl Acrylate/Itaconic Acid Hydrogels Optimized with Response Surface Methodology
IS  - 4
VL  - 7
DO  - 10.3390/gels7040225
ER  - 

@article{
author = "Antić, Katarina and Onjia, Antonije and Vasiljevic-Radovic, Dana and Veličković, Zlate and Tomić, Simonida",
year = "2021",
abstract = "The adsorption of Ni2+ ions from water solutions by using hydrogels based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA) was studied. Hydrogel synthesis was optimized with response surface methodology (RSM). The hydrogel with the best adsorption capacity towards Ni2+ ions was chosen for further experiments. The hydrogel was characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM) analysis before and after the adsorption of Ni2+ ions. Batch equilibrium experiments were conducted to investigate the influence of solution pH, hydrogel weight, ionic strength, adsorption time, temperature and initial concentration of nickel ions on the adsorption. Time-dependent adsorption fitted the best to the pseudo-second-order kinetic model. A thermodynamic study revealed that the adsorption was an exothermic and non-spontaneous process. Five isotherm models were studied, and the best fit was obtained with the Redlich-Peterson model. Consecutive adsorption/desorption studies indicated that the HEA/IA hydrogel can be efficiently used as a sorbent for the removal of Ni2+ ions from the water solution. This study develops a potential adsorbent for the effective removal of trace nickel ions.",
journal = "Gels",
title = "Removal of Nickel Ions from Aqueous Solutions by 2-Hydroxyethyl Acrylate/Itaconic Acid Hydrogels Optimized with Response Surface Methodology",
number = "4",
volume = "7",
doi = "10.3390/gels7040225"
}

Antić, K., Onjia, A., Vasiljevic-Radovic, D., Veličković, Z.,& Tomić, S.. (2021). Removal of Nickel Ions from Aqueous Solutions by 2-Hydroxyethyl Acrylate/Itaconic Acid Hydrogels Optimized with Response Surface Methodology. in Gels, 7(4).
https://doi.org/10.3390/gels7040225

Antić K, Onjia A, Vasiljevic-Radovic D, Veličković Z, Tomić S. Removal of Nickel Ions from Aqueous Solutions by 2-Hydroxyethyl Acrylate/Itaconic Acid Hydrogels Optimized with Response Surface Methodology. in Gels. 2021;7(4).
doi:10.3390/gels7040225 .

Antić, Katarina, Onjia, Antonije, Vasiljevic-Radovic, Dana, Veličković, Zlate, Tomić, Simonida, "Removal of Nickel Ions from Aqueous Solutions by 2-Hydroxyethyl Acrylate/Itaconic Acid Hydrogels Optimized with Response Surface Methodology" in Gels, 7, no. 4 (2021),
https://doi.org/10.3390/gels7040225 . .

TY  - JOUR
AU  - Vuković, Jovana
AU  - Perić-Grujić, Aleksandra
AU  - Mitić-Culafić, Dragana S.
AU  - Božić-Nedeljković, Biljana
AU  - Tomić, Simonida
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4345
AB  - Since the management of infections becomes prior global healthcare issue, the "post antibiotic era" requires innovative and interdisciplinary approach. As an alternative to widespread and, nowdays mostly uneffective, antibiotic treatment of infections, the series of hydrogels were developed and further investigated as novel antibacterial biomaterials. The hydrogels based on 2-hydroxyethyl acrylate and itaconic acid were synthesized and used for silver(I) ions incorporation. The structural, thermal and swelling characteristics were examined by Fourier transform infrared spectroscopy, differential scanning calorimetry, and swelling study conducted in wide range of pHs at 37 degrees C. Results confirmed the expected structure, while the glass transition temperatures (T-g) of the hydrogels were detected in range of 10-37 degrees C. The in vitro release study revealed suitability of these pH sensitive hydrogels as the systems for topical delivery of silver(I) ions. Performed MTT test and Comet assay proved biocompatibility of the hydrogels, as well as the absence of acute genotoxic effect on human fibroblast cells (MRC-5). The hydrogels exhibited satisfying antibacterial activity against methicillin sensitive Staphylococcus aureus (MSSA) and methicillin resistant Staphylococcus aureus (MRSA), indicating the capacity to treat the life-threatening infections.
PB  - Polymer Soc Korea, Seoul
T2  - Macromolecular Research
T1  - Antibacterial Activity of pH-Sensitive Silver(I)/Poly(2-hydroxyethyl acrylate/itaconic acid) Hydrogels
EP  - 389
IS  - 4
SP  - 382
VL  - 28
DO  - 10.1007/s13233-020-8050-z
ER  - 

@article{
author = "Vuković, Jovana and Perić-Grujić, Aleksandra and Mitić-Culafić, Dragana S. and Božić-Nedeljković, Biljana and Tomić, Simonida",
year = "2020",
abstract = "Since the management of infections becomes prior global healthcare issue, the "post antibiotic era" requires innovative and interdisciplinary approach. As an alternative to widespread and, nowdays mostly uneffective, antibiotic treatment of infections, the series of hydrogels were developed and further investigated as novel antibacterial biomaterials. The hydrogels based on 2-hydroxyethyl acrylate and itaconic acid were synthesized and used for silver(I) ions incorporation. The structural, thermal and swelling characteristics were examined by Fourier transform infrared spectroscopy, differential scanning calorimetry, and swelling study conducted in wide range of pHs at 37 degrees C. Results confirmed the expected structure, while the glass transition temperatures (T-g) of the hydrogels were detected in range of 10-37 degrees C. The in vitro release study revealed suitability of these pH sensitive hydrogels as the systems for topical delivery of silver(I) ions. Performed MTT test and Comet assay proved biocompatibility of the hydrogels, as well as the absence of acute genotoxic effect on human fibroblast cells (MRC-5). The hydrogels exhibited satisfying antibacterial activity against methicillin sensitive Staphylococcus aureus (MSSA) and methicillin resistant Staphylococcus aureus (MRSA), indicating the capacity to treat the life-threatening infections.",
publisher = "Polymer Soc Korea, Seoul",
journal = "Macromolecular Research",
title = "Antibacterial Activity of pH-Sensitive Silver(I)/Poly(2-hydroxyethyl acrylate/itaconic acid) Hydrogels",
pages = "389-382",
number = "4",
volume = "28",
doi = "10.1007/s13233-020-8050-z"
}

Vuković, J., Perić-Grujić, A., Mitić-Culafić, D. S., Božić-Nedeljković, B.,& Tomić, S.. (2020). Antibacterial Activity of pH-Sensitive Silver(I)/Poly(2-hydroxyethyl acrylate/itaconic acid) Hydrogels. in Macromolecular Research
Polymer Soc Korea, Seoul., 28(4), 382-389.
https://doi.org/10.1007/s13233-020-8050-z

Vuković J, Perić-Grujić A, Mitić-Culafić DS, Božić-Nedeljković B, Tomić S. Antibacterial Activity of pH-Sensitive Silver(I)/Poly(2-hydroxyethyl acrylate/itaconic acid) Hydrogels. in Macromolecular Research. 2020;28(4):382-389.
doi:10.1007/s13233-020-8050-z .

Vuković, Jovana, Perić-Grujić, Aleksandra, Mitić-Culafić, Dragana S., Božić-Nedeljković, Biljana, Tomić, Simonida, "Antibacterial Activity of pH-Sensitive Silver(I)/Poly(2-hydroxyethyl acrylate/itaconic acid) Hydrogels" in Macromolecular Research, 28, no. 4 (2020):382-389,
https://doi.org/10.1007/s13233-020-8050-z . .

TY  - JOUR
AU  - Babić, Marija
AU  - Vukomanović, Marija
AU  - Stefanić, Martin
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4358
AB  - The strategy of combining polymers of natural and synthetic origin with inorganic components to use their unique synergistic effect for the development of the novel, sophisticated, and efficient 3D polymeric biomaterials, whose structure and properties mimic the extracellular matrix and simultaneously represent the suitable hydrogel platform for controlled drug release, is presented. The novel versatile 2-hydroxyethyl methacrylate/gelatin/alginate/iron(III) oxide based hydrogels are prepared by a simple but effective method-modified porogenation. Chemical composition, morphology, swelling capacity, porosity, mechanical properties, effects on cell viability, and in vitro degradation are tested to correlate the material's composition with the corresponding properties. The hydrogels show an interconnected porous microstructure, satisfactory mechanical strength, pH-sensitivity, and favorable curcumin release performances. The materials show good compatibility with healthy human fibroblast in cell culture judged by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, suggesting newly synthesized hydrogels as potentially a new generation of 3D biomaterials with tunable properties for versatile biomedical and pharmaceutical applications.
PB  - Wiley-VCH Verlag Gmbh, Weinheim
T2  - Macromolecular Chemistry and Physics
T1  - Controlled Curcumin Release from Hydrogel Scaffold Platform Based on 2-Hydroxyethyl Methacrylate/Gelatin/Alginate/Iron(III) Oxide
IS  - 20
SP  - 2000186
VL  - 221
DO  - 10.1002/macp.202000186
ER  - 

@article{
author = "Babić, Marija and Vukomanović, Marija and Stefanić, Martin and Nikodinović-Runić, Jasmina and Tomić, Simonida",
year = "2020",
abstract = "The strategy of combining polymers of natural and synthetic origin with inorganic components to use their unique synergistic effect for the development of the novel, sophisticated, and efficient 3D polymeric biomaterials, whose structure and properties mimic the extracellular matrix and simultaneously represent the suitable hydrogel platform for controlled drug release, is presented. The novel versatile 2-hydroxyethyl methacrylate/gelatin/alginate/iron(III) oxide based hydrogels are prepared by a simple but effective method-modified porogenation. Chemical composition, morphology, swelling capacity, porosity, mechanical properties, effects on cell viability, and in vitro degradation are tested to correlate the material's composition with the corresponding properties. The hydrogels show an interconnected porous microstructure, satisfactory mechanical strength, pH-sensitivity, and favorable curcumin release performances. The materials show good compatibility with healthy human fibroblast in cell culture judged by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, suggesting newly synthesized hydrogels as potentially a new generation of 3D biomaterials with tunable properties for versatile biomedical and pharmaceutical applications.",
publisher = "Wiley-VCH Verlag Gmbh, Weinheim",
journal = "Macromolecular Chemistry and Physics",
title = "Controlled Curcumin Release from Hydrogel Scaffold Platform Based on 2-Hydroxyethyl Methacrylate/Gelatin/Alginate/Iron(III) Oxide",
number = "20",
pages = "2000186",
volume = "221",
doi = "10.1002/macp.202000186"
}

Babić, M., Vukomanović, M., Stefanić, M., Nikodinović-Runić, J.,& Tomić, S.. (2020). Controlled Curcumin Release from Hydrogel Scaffold Platform Based on 2-Hydroxyethyl Methacrylate/Gelatin/Alginate/Iron(III) Oxide. in Macromolecular Chemistry and Physics
Wiley-VCH Verlag Gmbh, Weinheim., 221(20), 2000186.
https://doi.org/10.1002/macp.202000186

Babić M, Vukomanović M, Stefanić M, Nikodinović-Runić J, Tomić S. Controlled Curcumin Release from Hydrogel Scaffold Platform Based on 2-Hydroxyethyl Methacrylate/Gelatin/Alginate/Iron(III) Oxide. in Macromolecular Chemistry and Physics. 2020;221(20):2000186.
doi:10.1002/macp.202000186 .

Babić, Marija, Vukomanović, Marija, Stefanić, Martin, Nikodinović-Runić, Jasmina, Tomić, Simonida, "Controlled Curcumin Release from Hydrogel Scaffold Platform Based on 2-Hydroxyethyl Methacrylate/Gelatin/Alginate/Iron(III) Oxide" in Macromolecular Chemistry and Physics, 221, no. 20 (2020):2000186,
https://doi.org/10.1002/macp.202000186 . .

TY  - JOUR
AU  - Tomić, Simonida
AU  - Babić, Marija
AU  - Vuković, Jovana
AU  - Đokić, Lidija
AU  - Pavić, Aleksandar
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4379
AB  - We have studied the effect of compositions and methods of preparation on the physico-chemical and biocompatible behavior of the hydrogel matrices. Hydrogel matrices are synthesized by free radical polymerization of 2-hydroxyethyl methacrylate net and with gelatin. Highly porous hydrogel structures were obtained by porogenation, and by cryogenic treatment followed by freeze-drying. All samples were characterized for structural, morphological, absorption, degradation and in vitro (healthy human fibroblast cell line) and in vivo (zebrafishDanio rerio) biocompatible properties. The obtained results show that cryo samples, especially with gelatin show better, favorable absorption, morphological and biocompatible properties in comparison with PHEMA samples, which makes these materials highly attractive for biomedical uses.
PB  - Springer, Dordrecht
T2  - Journal of Polymer Research
T1  - Effect of composition and method of preparation of 2-hydroxyethyl methacrylate/gelatin hydrogels on biological in vitro (cell line) and in vivo (zebrafish) properties
IS  - 10
SP  - 305
VL  - 27
DO  - 10.1007/s10965-020-02219-w
ER  - 

@article{
author = "Tomić, Simonida and Babić, Marija and Vuković, Jovana and Đokić, Lidija and Pavić, Aleksandar and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "We have studied the effect of compositions and methods of preparation on the physico-chemical and biocompatible behavior of the hydrogel matrices. Hydrogel matrices are synthesized by free radical polymerization of 2-hydroxyethyl methacrylate net and with gelatin. Highly porous hydrogel structures were obtained by porogenation, and by cryogenic treatment followed by freeze-drying. All samples were characterized for structural, morphological, absorption, degradation and in vitro (healthy human fibroblast cell line) and in vivo (zebrafishDanio rerio) biocompatible properties. The obtained results show that cryo samples, especially with gelatin show better, favorable absorption, morphological and biocompatible properties in comparison with PHEMA samples, which makes these materials highly attractive for biomedical uses.",
publisher = "Springer, Dordrecht",
journal = "Journal of Polymer Research",
title = "Effect of composition and method of preparation of 2-hydroxyethyl methacrylate/gelatin hydrogels on biological in vitro (cell line) and in vivo (zebrafish) properties",
number = "10",
pages = "305",
volume = "27",
doi = "10.1007/s10965-020-02219-w"
}

Tomić, S., Babić, M., Vuković, J., Đokić, L., Pavić, A.,& Nikodinović-Runić, J.. (2020). Effect of composition and method of preparation of 2-hydroxyethyl methacrylate/gelatin hydrogels on biological in vitro (cell line) and in vivo (zebrafish) properties. in Journal of Polymer Research
Springer, Dordrecht., 27(10), 305.
https://doi.org/10.1007/s10965-020-02219-w

Tomić S, Babić M, Vuković J, Đokić L, Pavić A, Nikodinović-Runić J. Effect of composition and method of preparation of 2-hydroxyethyl methacrylate/gelatin hydrogels on biological in vitro (cell line) and in vivo (zebrafish) properties. in Journal of Polymer Research. 2020;27(10):305.
doi:10.1007/s10965-020-02219-w .

Tomić, Simonida, Babić, Marija, Vuković, Jovana, Đokić, Lidija, Pavić, Aleksandar, Nikodinović-Runić, Jasmina, "Effect of composition and method of preparation of 2-hydroxyethyl methacrylate/gelatin hydrogels on biological in vitro (cell line) and in vivo (zebrafish) properties" in Journal of Polymer Research, 27, no. 10 (2020):305,
https://doi.org/10.1007/s10965-020-02219-w . .

TY  - CHAP
AU  - Babić, Marija
AU  - Tomić, Simonida
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4581
AB  - In this study, three series of semi-interpenetrating networks were synthesized based on 2-hydroxyethyl methacrylate (HEMA), 2-hydroxyethyl acrylate (HEA), itaconic acid (IA), and poly(vinyl pyrrolidone) (PVP) as interpenetrating polymer. Syntheses were performed by free radical cross-linking/polymerization reaction. The first series represented hydrogels based on 2-hydroxyethyl methacrylate, poly(vinyl pyrrolidone), and itaconic acid, varying of poly(vinyl pyrrolidone) content. The second series of samples were hydrogels based on 2-hydroxyethyl acrylate, poly(vinyl pyrrolidone), and itaconic acid, varying of itaconic acid content. The third series of synthesized samples were based on 2-hydroxyethyl acrylate, poly(vinyl pyrrolidone), and itaconic acid, varying of poly(vinyl pyrrolidone) content. The content of component was varied in order to examine the influence on the structure, pH- and temperature-sensitive swelling-“intelligent” behavior, mechanical properties of hydrogels, as well as antimicrobial and biocompatible potential of hydrogels. Poly(vinyl pyrrolidone) is a linear polymer, which shows satisfactory biocompatibility and hydrophilicity. Itaconic acid gives pH-sensitive-“intelligent” behavior and better hydrophilicity. Hydrogels based on HEMA and HEA show excellent biocompatibility and satisfactory hydrophilicity. All three series of samples showed satisfactory cytocompatibility, as well as the antimicrobial potential tested against most common microbes. The results obtained and presented in this research can contribute to the development of new efficient polymeric biomaterials for biomedical applications.
PB  - Springer Singapore
T2  - Interpenetrating Polymer Network: Biomedical Applications
T1  - Semi-interpenetrating networks based on (Meth)acrylate, itaconic acid, and poly(vinyl pyrrolidone) hydrogels for biomedical applications
EP  - 288
SP  - 263
DO  - 10.1007/978-981-15-0283-5_10
ER  - 

@inbook{
author = "Babić, Marija and Tomić, Simonida",
year = "2020",
abstract = "In this study, three series of semi-interpenetrating networks were synthesized based on 2-hydroxyethyl methacrylate (HEMA), 2-hydroxyethyl acrylate (HEA), itaconic acid (IA), and poly(vinyl pyrrolidone) (PVP) as interpenetrating polymer. Syntheses were performed by free radical cross-linking/polymerization reaction. The first series represented hydrogels based on 2-hydroxyethyl methacrylate, poly(vinyl pyrrolidone), and itaconic acid, varying of poly(vinyl pyrrolidone) content. The second series of samples were hydrogels based on 2-hydroxyethyl acrylate, poly(vinyl pyrrolidone), and itaconic acid, varying of itaconic acid content. The third series of synthesized samples were based on 2-hydroxyethyl acrylate, poly(vinyl pyrrolidone), and itaconic acid, varying of poly(vinyl pyrrolidone) content. The content of component was varied in order to examine the influence on the structure, pH- and temperature-sensitive swelling-“intelligent” behavior, mechanical properties of hydrogels, as well as antimicrobial and biocompatible potential of hydrogels. Poly(vinyl pyrrolidone) is a linear polymer, which shows satisfactory biocompatibility and hydrophilicity. Itaconic acid gives pH-sensitive-“intelligent” behavior and better hydrophilicity. Hydrogels based on HEMA and HEA show excellent biocompatibility and satisfactory hydrophilicity. All three series of samples showed satisfactory cytocompatibility, as well as the antimicrobial potential tested against most common microbes. The results obtained and presented in this research can contribute to the development of new efficient polymeric biomaterials for biomedical applications.",
publisher = "Springer Singapore",
journal = "Interpenetrating Polymer Network: Biomedical Applications",
booktitle = "Semi-interpenetrating networks based on (Meth)acrylate, itaconic acid, and poly(vinyl pyrrolidone) hydrogels for biomedical applications",
pages = "288-263",
doi = "10.1007/978-981-15-0283-5_10"
}

Babić, M.,& Tomić, S.. (2020). Semi-interpenetrating networks based on (Meth)acrylate, itaconic acid, and poly(vinyl pyrrolidone) hydrogels for biomedical applications. in Interpenetrating Polymer Network: Biomedical Applications
Springer Singapore., 263-288.
https://doi.org/10.1007/978-981-15-0283-5_10

Babić M, Tomić S. Semi-interpenetrating networks based on (Meth)acrylate, itaconic acid, and poly(vinyl pyrrolidone) hydrogels for biomedical applications. in Interpenetrating Polymer Network: Biomedical Applications. 2020;:263-288.
doi:10.1007/978-981-15-0283-5_10 .

Babić, Marija, Tomić, Simonida, "Semi-interpenetrating networks based on (Meth)acrylate, itaconic acid, and poly(vinyl pyrrolidone) hydrogels for biomedical applications" in Interpenetrating Polymer Network: Biomedical Applications (2020):263-288,
https://doi.org/10.1007/978-981-15-0283-5_10 . .

TY  - CHAP
AU  - Tomić, Simonida
AU  - Vuković, Jovana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4580
AB  - The rapid emergence of antibiotic-resistant pathogens is becoming an imminent global public health problem. The development of novel antimicrobial materials aiming to prevent or control infections caused by these pathogens is a very important issue. Polymeric hydrogels are versatile materials, which can be a great alternative to conventional treatments of infections. Because of its high hydrophilicity, unique threedimensional network, fine biocompatibility, and cell adhesion, the hydrogels are suitable biomaterials for drug delivery in antimicrobial areas. The biocompatible nature of hydrogels makes them a convenient starting platform to develop selectively active antimicrobial materials. Hydrogels with antimicrobial properties can be designed by loading of known antimicrobial agents, or the material itself can be designed to possess inherent antimicrobial activity. The combination of polymeric hydrogels based on (meth)acrylate with metal ions (Ag, Cu, and Zn) is a simple and effective approach for obtaining multicomponent systems with diversefunctionalities. Silver (Ag+), copper (Cu2+), and zinc (Zn2+) ions have been loaded into hydrogels for antimicrobial applications. The incorporation of metal ions into hydrogels not only enhances the antimicrobial activity of hydrogels but also influences their swelling and release characteristics. Herein, we summarize recent findings in (meth)acrylate hydrogels containing metal ions with antimicrobial properties.
T2  - An Introduction to Antibacterial Properties
T1  - Antimicrobial properties of (meth)acrylate based hydrogels
EP  - 84
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4580
ER  - 

@inbook{
author = "Tomić, Simonida and Vuković, Jovana",
year = "2020",
abstract = "The rapid emergence of antibiotic-resistant pathogens is becoming an imminent global public health problem. The development of novel antimicrobial materials aiming to prevent or control infections caused by these pathogens is a very important issue. Polymeric hydrogels are versatile materials, which can be a great alternative to conventional treatments of infections. Because of its high hydrophilicity, unique threedimensional network, fine biocompatibility, and cell adhesion, the hydrogels are suitable biomaterials for drug delivery in antimicrobial areas. The biocompatible nature of hydrogels makes them a convenient starting platform to develop selectively active antimicrobial materials. Hydrogels with antimicrobial properties can be designed by loading of known antimicrobial agents, or the material itself can be designed to possess inherent antimicrobial activity. The combination of polymeric hydrogels based on (meth)acrylate with metal ions (Ag, Cu, and Zn) is a simple and effective approach for obtaining multicomponent systems with diversefunctionalities. Silver (Ag+), copper (Cu2+), and zinc (Zn2+) ions have been loaded into hydrogels for antimicrobial applications. The incorporation of metal ions into hydrogels not only enhances the antimicrobial activity of hydrogels but also influences their swelling and release characteristics. Herein, we summarize recent findings in (meth)acrylate hydrogels containing metal ions with antimicrobial properties.",
journal = "An Introduction to Antibacterial Properties",
booktitle = "Antimicrobial properties of (meth)acrylate based hydrogels",
pages = "84-41",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4580"
}

Tomić, S.,& Vuković, J.. (2020). Antimicrobial properties of (meth)acrylate based hydrogels. in An Introduction to Antibacterial Properties, 41-84.
https://hdl.handle.net/21.15107/rcub_technorep_4580

Tomić S, Vuković J. Antimicrobial properties of (meth)acrylate based hydrogels. in An Introduction to Antibacterial Properties. 2020;:41-84.
https://hdl.handle.net/21.15107/rcub_technorep_4580 .

Tomić, Simonida, Vuković, Jovana, "Antimicrobial properties of (meth)acrylate based hydrogels" in An Introduction to Antibacterial Properties (2020):41-84,
https://hdl.handle.net/21.15107/rcub_technorep_4580 .

TY  - JOUR
AU  - Filipović, Vuk
AU  - Babić, Marija
AU  - Gođevac, Dejan
AU  - Pavić, Aleksandar
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4076
AB  - New (beta-aminoester) hydrogels (PBAE) based on di(ethylene glycol)diacrylate and glycine are successfully synthesized and characterized for the first time in this work. PBAE macromers are obtained using Michael addition. By changing the diacrylate/amine stoichiometric ratio, but maintaining it  gt 1, samples with different chemical structure containing acrylate end-groups are obtained. The hydrogels are synthesized from macromers utilizing free radical polymerization. Chemical structure of macromers and hydrogels is confirmed by proton nuclear magnetic resonance, and Fourier transform infra-red spectroscopy. Swelling and degradation rates in physiological pH range change notably with pH and monomer molar ratio, validating pH sensitivity and zwitterionic behavior, which can be finely tuned by changing any of these parameters. In vitro cytotoxicity and in vivo acute embryotoxicity in zebrafish (Danio rerio) performed to assess the biocompatibility of the novel hydrogel materials and their degradation products reveal that materials are nontoxic and biocompatible. The Cephalexin in vitro drug release study, at pH values 2.20, 5.50, and 7.40, demonstrates pH-sensitive delivery with the release profiles effectively controlled by pH and the hydrogel composition. PBAE hydrogels exhibit great potential for a variety of biomedical applications, including tissue regeneration and intelligent drug delivery systems.
PB  - Wiley-VCH Verlag Gmbh, Weinheim
T2  - Macromolecular Chemistry and Physics
T1  - In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release
IS  - 17
SP  - 1900188
VL  - 220
DO  - 10.1002/macp.201900188
ER  - 

@article{
author = "Filipović, Vuk and Babić, Marija and Gođevac, Dejan and Pavić, Aleksandar and Nikodinović-Runić, Jasmina and Tomić, Simonida",
year = "2019",
abstract = "New (beta-aminoester) hydrogels (PBAE) based on di(ethylene glycol)diacrylate and glycine are successfully synthesized and characterized for the first time in this work. PBAE macromers are obtained using Michael addition. By changing the diacrylate/amine stoichiometric ratio, but maintaining it  gt 1, samples with different chemical structure containing acrylate end-groups are obtained. The hydrogels are synthesized from macromers utilizing free radical polymerization. Chemical structure of macromers and hydrogels is confirmed by proton nuclear magnetic resonance, and Fourier transform infra-red spectroscopy. Swelling and degradation rates in physiological pH range change notably with pH and monomer molar ratio, validating pH sensitivity and zwitterionic behavior, which can be finely tuned by changing any of these parameters. In vitro cytotoxicity and in vivo acute embryotoxicity in zebrafish (Danio rerio) performed to assess the biocompatibility of the novel hydrogel materials and their degradation products reveal that materials are nontoxic and biocompatible. The Cephalexin in vitro drug release study, at pH values 2.20, 5.50, and 7.40, demonstrates pH-sensitive delivery with the release profiles effectively controlled by pH and the hydrogel composition. PBAE hydrogels exhibit great potential for a variety of biomedical applications, including tissue regeneration and intelligent drug delivery systems.",
publisher = "Wiley-VCH Verlag Gmbh, Weinheim",
journal = "Macromolecular Chemistry and Physics",
title = "In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release",
number = "17",
pages = "1900188",
volume = "220",
doi = "10.1002/macp.201900188"
}

Filipović, V., Babić, M., Gođevac, D., Pavić, A., Nikodinović-Runić, J.,& Tomić, S.. (2019). In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release. in Macromolecular Chemistry and Physics
Wiley-VCH Verlag Gmbh, Weinheim., 220(17), 1900188.
https://doi.org/10.1002/macp.201900188

Filipović V, Babić M, Gođevac D, Pavić A, Nikodinović-Runić J, Tomić S. In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release. in Macromolecular Chemistry and Physics. 2019;220(17):1900188.
doi:10.1002/macp.201900188 .

Filipović, Vuk, Babić, Marija, Gođevac, Dejan, Pavić, Aleksandar, Nikodinović-Runić, Jasmina, Tomić, Simonida, "In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release" in Macromolecular Chemistry and Physics, 220, no. 17 (2019):1900188,
https://doi.org/10.1002/macp.201900188 . .

TY  - JOUR
AU  - Babić, Marija
AU  - Božić, Bojan
AU  - Božić, Biljana
AU  - Ušćumlić, Gordana
AU  - Tomić, Simonida
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3979
AB  - The discovery of novel biodegradable biomaterials able to support and control cellular activity as well as development of an enhanced and efficient method for their fabrication, are of paramount importance in the field of tissue engineering. This study highlights the design of novel degradable hydrogels based on gelatin and hydroxyethyl (meth)acrylates using the innovative combined two-step sequential microwave-assisted and UV photo-polymerization technique. Chemical composition, morphology, swelling capacity and degradation rate of the synthesized hydrogels were evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), swelling and weight loss measurements. As an initial step for evaluation of performance of the hydrogels in the biological environment, the in vitro biocompatibility of these hydrogels, was evaluated using L929 mouse fibroblasts. Obtained results demonstrated that the hydrogels possess a porous morphology with interconnected pores, 50% in vitro degradation after 7 months, and satisfied biocompatibility on L929 fibroblast cells. These unique performances of the hydrogels make them promising candidates for in vivo evaluation in clinical studies aiming at tissue regeneration.
PB  - Elsevier Science Bv, Amsterdam
T2  - Materials Letters
T1  - The innovative combined microwave-assisted and photo-polymerization technique for synthesis of the novel degradable hydroxyethyl (meth)acrylate/gelatin based scaffolds
EP  - 240
SP  - 236
VL  - 213
DO  - 10.1016/j.matlet.2017.11.087
ER  - 

@article{
author = "Babić, Marija and Božić, Bojan and Božić, Biljana and Ušćumlić, Gordana and Tomić, Simonida",
year = "2018",
abstract = "The discovery of novel biodegradable biomaterials able to support and control cellular activity as well as development of an enhanced and efficient method for their fabrication, are of paramount importance in the field of tissue engineering. This study highlights the design of novel degradable hydrogels based on gelatin and hydroxyethyl (meth)acrylates using the innovative combined two-step sequential microwave-assisted and UV photo-polymerization technique. Chemical composition, morphology, swelling capacity and degradation rate of the synthesized hydrogels were evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), swelling and weight loss measurements. As an initial step for evaluation of performance of the hydrogels in the biological environment, the in vitro biocompatibility of these hydrogels, was evaluated using L929 mouse fibroblasts. Obtained results demonstrated that the hydrogels possess a porous morphology with interconnected pores, 50% in vitro degradation after 7 months, and satisfied biocompatibility on L929 fibroblast cells. These unique performances of the hydrogels make them promising candidates for in vivo evaluation in clinical studies aiming at tissue regeneration.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Materials Letters",
title = "The innovative combined microwave-assisted and photo-polymerization technique for synthesis of the novel degradable hydroxyethyl (meth)acrylate/gelatin based scaffolds",
pages = "240-236",
volume = "213",
doi = "10.1016/j.matlet.2017.11.087"
}

Babić, M., Božić, B., Božić, B., Ušćumlić, G.,& Tomić, S.. (2018). The innovative combined microwave-assisted and photo-polymerization technique for synthesis of the novel degradable hydroxyethyl (meth)acrylate/gelatin based scaffolds. in Materials Letters
Elsevier Science Bv, Amsterdam., 213, 236-240.
https://doi.org/10.1016/j.matlet.2017.11.087

Babić M, Božić B, Božić B, Ušćumlić G, Tomić S. The innovative combined microwave-assisted and photo-polymerization technique for synthesis of the novel degradable hydroxyethyl (meth)acrylate/gelatin based scaffolds. in Materials Letters. 2018;213:236-240.
doi:10.1016/j.matlet.2017.11.087 .

Babić, Marija, Božić, Bojan, Božić, Biljana, Ušćumlić, Gordana, Tomić, Simonida, "The innovative combined microwave-assisted and photo-polymerization technique for synthesis of the novel degradable hydroxyethyl (meth)acrylate/gelatin based scaffolds" in Materials Letters, 213 (2018):236-240,
https://doi.org/10.1016/j.matlet.2017.11.087 . .

TY  - CONF
AU  - Filipović, Vuk
AU  - Marković, Maja
AU  - Tomić, Simonida
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7370
AB  - Hydrogels represent three-dimensional hydrophilic polymer networks, capable of absorbing large quantities of water1. Due to their tissue-like characteristics, hydrogels have been widely used for a variety of biomedical and pharmaceutical applications. The synthesis and characterization of novel semi-degradable, pH and temperature sensitive hydrogels is presented in this work. These new functional hydrogels represent copolymers based on 2-hydroxyethyl methacrylate (HEMA) and 2-hydroxyethyl acrylate (HEA), crosslinked using poly(β-amino ester) (PBAE) degradable macromer. PBAE crosslinker was synthesized by Michael addition reaction of diethylene glycol diacrylate (DEGDA) and piperazine. PBAE chemical structure was confirmed by proton nuclear magnetic resonance (1H NMR) and Fourier transform infrared (FTIR) spectroscopy. HEMA/HEA/PBAE hydrogels were synthesized by free radical copolymerization, by varying the monomer ratio. Structural characterization of hydrogels was carried out using Fourier transform infrared spectroscopy (FTIR). Swelling studies, performed in different pH buffers, ranging from 2.00 to 7.40, and different temperature values, ranging from 20 to 55°C, showed the pH and temperature sensitivity of the hydrogels. From the obtained results it can also be concluded that the swelling and degradation rates of new hydrogels can easily be adjusted by altering the comonomer ratio, making them a class of materials that is particularly suitable for potential drug delivery and tissue engineering applications.
PB  - Beograd : Srpsko hemijsko društvo = Serbian Chemical Society
C3  - Kratki izvodi radova / Šesta konferencija mladih hemičara Srbije, Beograd, 27. oktobar 2018. = Book of Abstracts / Sixth Conference of Young Chemists of Serbia, Belgrade, 27th October 2018
T1  - Synthesis and characterization of novel semi-degradable, pH and temperature sensitive hydrogels based on 2-hydroxyethyl methacrylate and 2-hydroxyethyl acrylate
SP  - 21
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7370
ER  - 

@conference{
author = "Filipović, Vuk and Marković, Maja and Tomić, Simonida",
year = "2018",
abstract = "Hydrogels represent three-dimensional hydrophilic polymer networks, capable of absorbing large quantities of water1. Due to their tissue-like characteristics, hydrogels have been widely used for a variety of biomedical and pharmaceutical applications. The synthesis and characterization of novel semi-degradable, pH and temperature sensitive hydrogels is presented in this work. These new functional hydrogels represent copolymers based on 2-hydroxyethyl methacrylate (HEMA) and 2-hydroxyethyl acrylate (HEA), crosslinked using poly(β-amino ester) (PBAE) degradable macromer. PBAE crosslinker was synthesized by Michael addition reaction of diethylene glycol diacrylate (DEGDA) and piperazine. PBAE chemical structure was confirmed by proton nuclear magnetic resonance (1H NMR) and Fourier transform infrared (FTIR) spectroscopy. HEMA/HEA/PBAE hydrogels were synthesized by free radical copolymerization, by varying the monomer ratio. Structural characterization of hydrogels was carried out using Fourier transform infrared spectroscopy (FTIR). Swelling studies, performed in different pH buffers, ranging from 2.00 to 7.40, and different temperature values, ranging from 20 to 55°C, showed the pH and temperature sensitivity of the hydrogels. From the obtained results it can also be concluded that the swelling and degradation rates of new hydrogels can easily be adjusted by altering the comonomer ratio, making them a class of materials that is particularly suitable for potential drug delivery and tissue engineering applications.",
publisher = "Beograd : Srpsko hemijsko društvo = Serbian Chemical Society",
journal = "Kratki izvodi radova / Šesta konferencija mladih hemičara Srbije, Beograd, 27. oktobar 2018. = Book of Abstracts / Sixth Conference of Young Chemists of Serbia, Belgrade, 27th October 2018",
title = "Synthesis and characterization of novel semi-degradable, pH and temperature sensitive hydrogels based on 2-hydroxyethyl methacrylate and 2-hydroxyethyl acrylate",
pages = "21",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7370"
}

Filipović, V., Marković, M.,& Tomić, S.. (2018). Synthesis and characterization of novel semi-degradable, pH and temperature sensitive hydrogels based on 2-hydroxyethyl methacrylate and 2-hydroxyethyl acrylate. in Kratki izvodi radova / Šesta konferencija mladih hemičara Srbije, Beograd, 27. oktobar 2018. = Book of Abstracts / Sixth Conference of Young Chemists of Serbia, Belgrade, 27th October 2018
Beograd : Srpsko hemijsko društvo = Serbian Chemical Society., 21.
https://hdl.handle.net/21.15107/rcub_technorep_7370

Filipović V, Marković M, Tomić S. Synthesis and characterization of novel semi-degradable, pH and temperature sensitive hydrogels based on 2-hydroxyethyl methacrylate and 2-hydroxyethyl acrylate. in Kratki izvodi radova / Šesta konferencija mladih hemičara Srbije, Beograd, 27. oktobar 2018. = Book of Abstracts / Sixth Conference of Young Chemists of Serbia, Belgrade, 27th October 2018. 2018;:21.
https://hdl.handle.net/21.15107/rcub_technorep_7370 .

Filipović, Vuk, Marković, Maja, Tomić, Simonida, "Synthesis and characterization of novel semi-degradable, pH and temperature sensitive hydrogels based on 2-hydroxyethyl methacrylate and 2-hydroxyethyl acrylate" in Kratki izvodi radova / Šesta konferencija mladih hemičara Srbije, Beograd, 27. oktobar 2018. = Book of Abstracts / Sixth Conference of Young Chemists of Serbia, Belgrade, 27th October 2018 (2018):21,
https://hdl.handle.net/21.15107/rcub_technorep_7370 .

TY  - JOUR
AU  - Filipović, Vuk
AU  - Božić-Nedeljković, Biljana
AU  - Vukomanović, Marija
AU  - Tomić, Simonida
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3874
AB  - Gelatin hydrogels have great potential in regenerative medicine but their weak mechanical properties are a major drawback for the load-bearing applications, such as scaffolds for tissue engineering. To overcome this deficiency, novel biodegradable hydrogels with improved mechanical properties were prepared by combining gelatine with 2-hydroxyethyl methacrylate (HEMA), using a double network synthetic procedure. The first, superporous and mechanically strong network, was obtained by free radical polymerization of HEMA at cryogenic temperature, in the presence of gelatin. Degradable poly (beta-amino ester) (PBAE) macromers of different chemical composition or molecular weight were used as crosslinkers to introduce hydrolytically labile bonds in PHEMA. The second gelatin network was formed by crosslinking gelatin with glutaraldehyde. For comparison, a set of biodegradable PHEMA networks was obtained by polymerization of HEMA at cryogenic temperature. All samples were characterized revealing that mechanical strength, swelling behavior and degradation rate as well as high biocompatibility of new IPNs are in accordance with values required for scaffolds in tissue engineering applications and that tuning of these properties is accomplished by simply using different PBAE macromers.
PB  - Elsevier Sci Ltd, Oxford
T2  - Polymer Testing
T1  - Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers
EP  - 278
SP  - 270
VL  - 68
DO  - 10.1016/j.polymertesting.2018.04.024
ER  - 

@article{
author = "Filipović, Vuk and Božić-Nedeljković, Biljana and Vukomanović, Marija and Tomić, Simonida",
year = "2018",
abstract = "Gelatin hydrogels have great potential in regenerative medicine but their weak mechanical properties are a major drawback for the load-bearing applications, such as scaffolds for tissue engineering. To overcome this deficiency, novel biodegradable hydrogels with improved mechanical properties were prepared by combining gelatine with 2-hydroxyethyl methacrylate (HEMA), using a double network synthetic procedure. The first, superporous and mechanically strong network, was obtained by free radical polymerization of HEMA at cryogenic temperature, in the presence of gelatin. Degradable poly (beta-amino ester) (PBAE) macromers of different chemical composition or molecular weight were used as crosslinkers to introduce hydrolytically labile bonds in PHEMA. The second gelatin network was formed by crosslinking gelatin with glutaraldehyde. For comparison, a set of biodegradable PHEMA networks was obtained by polymerization of HEMA at cryogenic temperature. All samples were characterized revealing that mechanical strength, swelling behavior and degradation rate as well as high biocompatibility of new IPNs are in accordance with values required for scaffolds in tissue engineering applications and that tuning of these properties is accomplished by simply using different PBAE macromers.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Polymer Testing",
title = "Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers",
pages = "278-270",
volume = "68",
doi = "10.1016/j.polymertesting.2018.04.024"
}

Filipović, V., Božić-Nedeljković, B., Vukomanović, M.,& Tomić, S.. (2018). Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers. in Polymer Testing
Elsevier Sci Ltd, Oxford., 68, 270-278.
https://doi.org/10.1016/j.polymertesting.2018.04.024

Filipović V, Božić-Nedeljković B, Vukomanović M, Tomić S. Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers. in Polymer Testing. 2018;68:270-278.
doi:10.1016/j.polymertesting.2018.04.024 .

Filipović, Vuk, Božić-Nedeljković, Biljana, Vukomanović, Marija, Tomić, Simonida, "Biocompatible and degradable scaffolds based on 2-hydroxyethyl methacrylate, gelatin and poly(beta amino ester) crosslinkers" in Polymer Testing, 68 (2018):270-278,
https://doi.org/10.1016/j.polymertesting.2018.04.024 . .

TY  - JOUR
AU  - Krezović, Bojana D.
AU  - Miljković, Miona
AU  - Stojanović, Sanja T.
AU  - Najman, Stevo
AU  - Filipović, Jovanka
AU  - Tomić, Simonida
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3709
AB  - The aim of this work was to synthesize a series of semi-interpenetrating polymer networks (semi-IPNs) of 2-hydroxyethyl acrylate and itaconic acid, in the presence of poly(N-vinylpyrrolidone). Samples were synthesized by free radical copolymerization with constant PVP and variable IA content and characterized for structural, morphological, thermal, swelling, drug release, antibacterial and cytotoxic properties. The chemical structure of samples was confirmed by Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) was used to examine morphology of samples and glass transition temperatures were determined by differential scanning calorimetry (DCS). The pH and temperature sensitivity was confirmed by measuring the dependence of the degree of swelling on pH and temperature. All samples show volume phase transition temperature (VPTT) around 47 degrees C. Drug release profiles were investigated using vitamin B-3 as a model drug. The vitamin B-3 transport mechanism was studied by fitting experimental data to five different model equations and calculating the corresponding parameters. It was also observed that IA content has a marked influence on the release profiles of vitamin B-3, so the rate of drug release can be designed by changing the HEA/IA ratio. Cell viability, performed using MTT test, was high for all samples and all concentration of extract used. The antibacterial activity of hydrogels was determined against Pseudomonas aeruginosa. It turns out that IA content and time of exposure have an influence on the antibacterial potential. All samples showed satisfied cell viability. Due to these characteristics, P(HEA/IA)/PVP semi-IPNs represent interesting biomaterials for biomedical applications.
PB  - Inst Chemical Engineers, Rugby
T2  - Chemical Engineering Research & Design
T1  - Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels
EP  - 380
SP  - 368
VL  - 121
DO  - 10.1016/j.cherd.2017.03.030
ER  - 

@article{
author = "Krezović, Bojana D. and Miljković, Miona and Stojanović, Sanja T. and Najman, Stevo and Filipović, Jovanka and Tomić, Simonida",
year = "2017",
abstract = "The aim of this work was to synthesize a series of semi-interpenetrating polymer networks (semi-IPNs) of 2-hydroxyethyl acrylate and itaconic acid, in the presence of poly(N-vinylpyrrolidone). Samples were synthesized by free radical copolymerization with constant PVP and variable IA content and characterized for structural, morphological, thermal, swelling, drug release, antibacterial and cytotoxic properties. The chemical structure of samples was confirmed by Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) was used to examine morphology of samples and glass transition temperatures were determined by differential scanning calorimetry (DCS). The pH and temperature sensitivity was confirmed by measuring the dependence of the degree of swelling on pH and temperature. All samples show volume phase transition temperature (VPTT) around 47 degrees C. Drug release profiles were investigated using vitamin B-3 as a model drug. The vitamin B-3 transport mechanism was studied by fitting experimental data to five different model equations and calculating the corresponding parameters. It was also observed that IA content has a marked influence on the release profiles of vitamin B-3, so the rate of drug release can be designed by changing the HEA/IA ratio. Cell viability, performed using MTT test, was high for all samples and all concentration of extract used. The antibacterial activity of hydrogels was determined against Pseudomonas aeruginosa. It turns out that IA content and time of exposure have an influence on the antibacterial potential. All samples showed satisfied cell viability. Due to these characteristics, P(HEA/IA)/PVP semi-IPNs represent interesting biomaterials for biomedical applications.",
publisher = "Inst Chemical Engineers, Rugby",
journal = "Chemical Engineering Research & Design",
title = "Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels",
pages = "380-368",
volume = "121",
doi = "10.1016/j.cherd.2017.03.030"
}

Krezović, B. D., Miljković, M., Stojanović, S. T., Najman, S., Filipović, J.,& Tomić, S.. (2017). Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels. in Chemical Engineering Research & Design
Inst Chemical Engineers, Rugby., 121, 368-380.
https://doi.org/10.1016/j.cherd.2017.03.030

Krezović BD, Miljković M, Stojanović ST, Najman S, Filipović J, Tomić S. Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels. in Chemical Engineering Research & Design. 2017;121:368-380.
doi:10.1016/j.cherd.2017.03.030 .

Krezović, Bojana D., Miljković, Miona, Stojanović, Sanja T., Najman, Stevo, Filipović, Jovanka, Tomić, Simonida, "Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels" in Chemical Engineering Research & Design, 121 (2017):368-380,
https://doi.org/10.1016/j.cherd.2017.03.030 . .

TY  - JOUR
AU  - Babić, Marija
AU  - Božić, Bojan
AU  - Božić, Biljana
AU  - Filipović, Jovanka
AU  - Ušćumlić, Gordana
AU  - Tomić, Simonida
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3409
AB  - A series of dual-sensitive poly(2-hydroxypropyl acrylate/itaconic acid) (P(HPA/IA)) hydrogels were synthesized and evaluated as potential highly effective antiproliferative drug delivery system. Investigated hydrophobic antiproliferative agent, Ni(II) complex with Oxaprozin, was successfully synthesized and efficiently loaded into the'"intelligent" P(HPA/IA) hydrogels. Swelling studies showed that loaded agent did not annul pH- and temperature-sensitivity of the investigated hydrogels. In vitro antiproliferative activity of investigated complex against human cervical (HeLa) and melanoma cancer (FemX) cell lines was tested. The results of in vitro release study at different pH values confirmed synthesized hydrogels loaded with investigated complex as a highly effective pH-triggered drug delivery system for the advanced anticancer therapy as well as for the targeted treatment of intestine/colon cancers.
PB  - Elsevier, Amsterdam
T2  - Materials Letters
T1  - Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin
EP  - 217
SP  - 214
VL  - 163
DO  - 10.1016/j.matlet.2015.10.078
ER  - 

@article{
author = "Babić, Marija and Božić, Bojan and Božić, Biljana and Filipović, Jovanka and Ušćumlić, Gordana and Tomić, Simonida",
year = "2016",
abstract = "A series of dual-sensitive poly(2-hydroxypropyl acrylate/itaconic acid) (P(HPA/IA)) hydrogels were synthesized and evaluated as potential highly effective antiproliferative drug delivery system. Investigated hydrophobic antiproliferative agent, Ni(II) complex with Oxaprozin, was successfully synthesized and efficiently loaded into the'"intelligent" P(HPA/IA) hydrogels. Swelling studies showed that loaded agent did not annul pH- and temperature-sensitivity of the investigated hydrogels. In vitro antiproliferative activity of investigated complex against human cervical (HeLa) and melanoma cancer (FemX) cell lines was tested. The results of in vitro release study at different pH values confirmed synthesized hydrogels loaded with investigated complex as a highly effective pH-triggered drug delivery system for the advanced anticancer therapy as well as for the targeted treatment of intestine/colon cancers.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Letters",
title = "Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin",
pages = "217-214",
volume = "163",
doi = "10.1016/j.matlet.2015.10.078"
}

Babić, M., Božić, B., Božić, B., Filipović, J., Ušćumlić, G.,& Tomić, S.. (2016). Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin. in Materials Letters
Elsevier, Amsterdam., 163, 214-217.
https://doi.org/10.1016/j.matlet.2015.10.078

Babić M, Božić B, Božić B, Filipović J, Ušćumlić G, Tomić S. Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin. in Materials Letters. 2016;163:214-217.
doi:10.1016/j.matlet.2015.10.078 .

Babić, Marija, Božić, Bojan, Božić, Biljana, Filipović, Jovanka, Ušćumlić, Gordana, Tomić, Simonida, "Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin" in Materials Letters, 163 (2016):214-217,
https://doi.org/10.1016/j.matlet.2015.10.078 . .

TY  - CONF
AU  - Tomić, Simonida
AU  - Babić, Marija
AU  - Vuković, Jovana
AU  - Perišić, Marija D.
AU  - Filipović, Vuk
AU  - Davidović, Slađana
AU  - Filipović, Jovanka M.
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3296
AB  - In this study, superporous hydrogels were synthesized by free radical polymerization of 2-hydroxyethyl methacrylate without and in the presence of gelatin. Highly porous hydrogel structures were obtained by two different techniques: using a gas blowing agent, sodium bicarbonate, and a cryogenic treatment followed by freeze-drying. After the gel synthesis, gelatin molecules were covalently immobilised onto PHEMA via glytaraldehyde activation. All samples were characterized for morphological, mechanical, swelling and antibacterial properties. The results obtained show that samples with gelatin show better properties in comparison with PHEMA samples, which make these materials highly attractive for developing hydrogel scaffolds for tissue regeneration.
PB  - Amer Inst Physics, Melville
C3  - VIII International Conference on Times of Polymers and Composites: From Aerospace to Nanotechnology
T1  - 2-Hydroxyethyl Metahcrylate/Gelatin based Superporous Hydrogels for Tissue Regeneration
SP  - 4949668
VL  - 1736
DO  - 10.1063/1.4949668
ER  - 

@conference{
author = "Tomić, Simonida and Babić, Marija and Vuković, Jovana and Perišić, Marija D. and Filipović, Vuk and Davidović, Slađana and Filipović, Jovanka M.",
year = "2016",
abstract = "In this study, superporous hydrogels were synthesized by free radical polymerization of 2-hydroxyethyl methacrylate without and in the presence of gelatin. Highly porous hydrogel structures were obtained by two different techniques: using a gas blowing agent, sodium bicarbonate, and a cryogenic treatment followed by freeze-drying. After the gel synthesis, gelatin molecules were covalently immobilised onto PHEMA via glytaraldehyde activation. All samples were characterized for morphological, mechanical, swelling and antibacterial properties. The results obtained show that samples with gelatin show better properties in comparison with PHEMA samples, which make these materials highly attractive for developing hydrogel scaffolds for tissue regeneration.",
publisher = "Amer Inst Physics, Melville",
journal = "VIII International Conference on Times of Polymers and Composites: From Aerospace to Nanotechnology",
title = "2-Hydroxyethyl Metahcrylate/Gelatin based Superporous Hydrogels for Tissue Regeneration",
pages = "4949668",
volume = "1736",
doi = "10.1063/1.4949668"
}

Tomić, S., Babić, M., Vuković, J., Perišić, M. D., Filipović, V., Davidović, S.,& Filipović, J. M.. (2016). 2-Hydroxyethyl Metahcrylate/Gelatin based Superporous Hydrogels for Tissue Regeneration. in VIII International Conference on Times of Polymers and Composites: From Aerospace to Nanotechnology
Amer Inst Physics, Melville., 1736, 4949668.
https://doi.org/10.1063/1.4949668

Tomić S, Babić M, Vuković J, Perišić MD, Filipović V, Davidović S, Filipović JM. 2-Hydroxyethyl Metahcrylate/Gelatin based Superporous Hydrogels for Tissue Regeneration. in VIII International Conference on Times of Polymers and Composites: From Aerospace to Nanotechnology. 2016;1736:4949668.
doi:10.1063/1.4949668 .

Tomić, Simonida, Babić, Marija, Vuković, Jovana, Perišić, Marija D., Filipović, Vuk, Davidović, Slađana, Filipović, Jovanka M., "2-Hydroxyethyl Metahcrylate/Gelatin based Superporous Hydrogels for Tissue Regeneration" in VIII International Conference on Times of Polymers and Composites: From Aerospace to Nanotechnology, 1736 (2016):4949668,
https://doi.org/10.1063/1.4949668 . .

TY  - JOUR
AU  - Antić, Katarina
AU  - Babić, Marija
AU  - Vuković, Jovana
AU  - Onjia, Antonije
AU  - Filipović, Jovanka M.
AU  - Tomić, Simonida
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3231
AB  - A series of poly(2-hydroxyethyl acrylate-co-itaconic acid), P(HEA/IA), hydrogels with different HEA/IA ratio, were synthesized using free radical crosslinking/copolymerization and investigated as sorbents for Pb2+ from aqueous solutions. Hydrogels were characterized using DMA, FTIR, DSC, SEM and AFM. The adsorption was found to be highly dependent on hydrogel composition, solution pH, sorbent weight, ionic strength and contact time. Five isotherm models, Langmuir, Freundlich, Redlich-Peterson, Temkin and Dubinin-Radushkevich, were applied to the sorption data. The best fit was obtained with Redlich-Peterson isotherm. The separation factor, RL, value indicated favorable sorption for Pb2+. The maximum sorption capacities were 392.2 and 409.8 mg/g for P(HEA/2IA) and P(HEA/10IA), respectively. Kinetic data showed best fit with pseudo-second-order model. Thermodynamic studies revealed that the reaction was exothermic and proceeds with a decrease in entropy. Moreover, P(HEA/IA) hydrogel showed the most pronounced sorption toward Pb2+ from environment containing Cu2+, Zn2+, Cd2+, Ni2+ and Co2+. Sorption/desorption experiments, showed that the P(HEA/IA) hydrogels could be reused without significant loss of the initial properties even after three adsorption-desorption cycles.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Removal of Pb2+ from aqueous solution by P(HEA/IA) hydrogels
EP  - 705
IS  - 6
SP  - 695
VL  - 70
DO  - 10.2298/HEMIND151225006A
ER  - 

@article{
author = "Antić, Katarina and Babić, Marija and Vuković, Jovana and Onjia, Antonije and Filipović, Jovanka M. and Tomić, Simonida",
year = "2016",
abstract = "A series of poly(2-hydroxyethyl acrylate-co-itaconic acid), P(HEA/IA), hydrogels with different HEA/IA ratio, were synthesized using free radical crosslinking/copolymerization and investigated as sorbents for Pb2+ from aqueous solutions. Hydrogels were characterized using DMA, FTIR, DSC, SEM and AFM. The adsorption was found to be highly dependent on hydrogel composition, solution pH, sorbent weight, ionic strength and contact time. Five isotherm models, Langmuir, Freundlich, Redlich-Peterson, Temkin and Dubinin-Radushkevich, were applied to the sorption data. The best fit was obtained with Redlich-Peterson isotherm. The separation factor, RL, value indicated favorable sorption for Pb2+. The maximum sorption capacities were 392.2 and 409.8 mg/g for P(HEA/2IA) and P(HEA/10IA), respectively. Kinetic data showed best fit with pseudo-second-order model. Thermodynamic studies revealed that the reaction was exothermic and proceeds with a decrease in entropy. Moreover, P(HEA/IA) hydrogel showed the most pronounced sorption toward Pb2+ from environment containing Cu2+, Zn2+, Cd2+, Ni2+ and Co2+. Sorption/desorption experiments, showed that the P(HEA/IA) hydrogels could be reused without significant loss of the initial properties even after three adsorption-desorption cycles.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Removal of Pb2+ from aqueous solution by P(HEA/IA) hydrogels",
pages = "705-695",
number = "6",
volume = "70",
doi = "10.2298/HEMIND151225006A"
}

Antić, K., Babić, M., Vuković, J., Onjia, A., Filipović, J. M.,& Tomić, S.. (2016). Removal of Pb2+ from aqueous solution by P(HEA/IA) hydrogels. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 70(6), 695-705.
https://doi.org/10.2298/HEMIND151225006A

Antić K, Babić M, Vuković J, Onjia A, Filipović JM, Tomić S. Removal of Pb2+ from aqueous solution by P(HEA/IA) hydrogels. in Hemijska industrija. 2016;70(6):695-705.
doi:10.2298/HEMIND151225006A .

Antić, Katarina, Babić, Marija, Vuković, Jovana, Onjia, Antonije, Filipović, Jovanka M., Tomić, Simonida, "Removal of Pb2+ from aqueous solution by P(HEA/IA) hydrogels" in Hemijska industrija, 70, no. 6 (2016):695-705,
https://doi.org/10.2298/HEMIND151225006A . .

TY  - JOUR
AU  - Vuković, Jovana
AU  - Babić, Marija
AU  - Antić, Katarina
AU  - Filipović, Jovanka M.
AU  - Stojanović, Sanja T.
AU  - Najman, Stevo
AU  - Tomić, Simonida
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3352
AB  - In order to evaluate biological response and potential toxicity prior to clinical use as wound dressing materials, three series of hydrogels, based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA), unloaded, with incorporated copper(II) ions and reduced copper, were subjected to cytotoxicity testing on L929 cell line (mouse fibroblasts). MTT test revealed proper fibroblast growth in the presence of tested hydrogels, with an absence of any acute toxic effects. Mechanical properties of all three hydrogel series were studied by dynamic mechanical analysis (DMA) and obtained results indicated dominant elastic over viscous behaviour, which supports their application in wound management. The temperature sensitive behaviour of the hydrogels was detected in swelling study conducted in wide range of temperature values 25-50 degrees C, in buffer pH 7.40. The hydrogels exhibited excellent microbial barrier characteristics against Pseudomonas aeruginosa, reducing the risk of bacterial infection in wound bed.
PB  - Elsevier Science Sa, Lausanne
T2  - Materials Chemistry and Physics
T1  - In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management
EP  - 163
SP  - 158
VL  - 175
DO  - 10.1016/j.matchemphys.2016.03.009
ER  - 

@article{
author = "Vuković, Jovana and Babić, Marija and Antić, Katarina and Filipović, Jovanka M. and Stojanović, Sanja T. and Najman, Stevo and Tomić, Simonida",
year = "2016",
abstract = "In order to evaluate biological response and potential toxicity prior to clinical use as wound dressing materials, three series of hydrogels, based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA), unloaded, with incorporated copper(II) ions and reduced copper, were subjected to cytotoxicity testing on L929 cell line (mouse fibroblasts). MTT test revealed proper fibroblast growth in the presence of tested hydrogels, with an absence of any acute toxic effects. Mechanical properties of all three hydrogel series were studied by dynamic mechanical analysis (DMA) and obtained results indicated dominant elastic over viscous behaviour, which supports their application in wound management. The temperature sensitive behaviour of the hydrogels was detected in swelling study conducted in wide range of temperature values 25-50 degrees C, in buffer pH 7.40. The hydrogels exhibited excellent microbial barrier characteristics against Pseudomonas aeruginosa, reducing the risk of bacterial infection in wound bed.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Materials Chemistry and Physics",
title = "In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management",
pages = "163-158",
volume = "175",
doi = "10.1016/j.matchemphys.2016.03.009"
}

Vuković, J., Babić, M., Antić, K., Filipović, J. M., Stojanović, S. T., Najman, S.,& Tomić, S.. (2016). In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management. in Materials Chemistry and Physics
Elsevier Science Sa, Lausanne., 175, 158-163.
https://doi.org/10.1016/j.matchemphys.2016.03.009

Vuković J, Babić M, Antić K, Filipović JM, Stojanović ST, Najman S, Tomić S. In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management. in Materials Chemistry and Physics. 2016;175:158-163.
doi:10.1016/j.matchemphys.2016.03.009 .

Vuković, Jovana, Babić, Marija, Antić, Katarina, Filipović, Jovanka M., Stojanović, Sanja T., Najman, Stevo, Tomić, Simonida, "In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management" in Materials Chemistry and Physics, 175 (2016):158-163,
https://doi.org/10.1016/j.matchemphys.2016.03.009 . .