TY  - JOUR
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Milić, Marija
AU  - Lađarević, Jelena
AU  - Onjia, Antonije
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7444
AB  - This study presents an innovative approach for the reuse and recycling of waste material, brewer’s spent grain (BSG) for
creating a novel green biocatalyst. The same BSG was utilized in several consecutive steps: initially, it served as a substrate
for the cultivation and production of laccase by a novel isolated fungal strain, Coriolopsis trogii 2SMKN, then, it was reused
as a carrier for laccase immobilization, aiding in the process of azo dye decolorization and fnally, reused as recycled BSG
for the second successful laccase immobilization for six guaiacol oxidation, contributing to a zero-waste strategy. The novel
fungal strain produced laccase with a maximum activity of 171.4 U/g after 6 days of solid-state fermentation using BSG
as a substrate. The obtained laccase exhibited excellent performance in the decolorization of azo dyes, both as a free and
immobilized, at high temperatures, without addition of harmful mediators, achieving maximum decolorization efciencies
of 99.0%, 71.2%, and 61.0% for Orange G (OG), Congo Red, and Eriochrome Black T (EBT), respectively. The immobilized
laccase on BSG was successfully reused across fve cycles of azo dye decolorization process. Notably, new green biocatalyst
outperformed commercial laccase from Aspergillus spp. in the decolorization of OG and EBT. GC-MS and LC-MS revealed
azo-dye degradation products and decomposition pathway. This analysis was complemented by antimicrobial and phytotoxicity tests, which confrmed the non-toxic nature of the degradation products, indicating the potential for safe environmental
disposal.
PB  - Springer
T2  - Environmental Science and Pollution Research
T1  - Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain
DO  - 10.1007/s11356-024-33367-x
ER  - 

@article{
author = "Ilić, Nevena and Davidović, Slađana and Milić, Marija and Lađarević, Jelena and Onjia, Antonije and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2024",
abstract = "This study presents an innovative approach for the reuse and recycling of waste material, brewer’s spent grain (BSG) for
creating a novel green biocatalyst. The same BSG was utilized in several consecutive steps: initially, it served as a substrate
for the cultivation and production of laccase by a novel isolated fungal strain, Coriolopsis trogii 2SMKN, then, it was reused
as a carrier for laccase immobilization, aiding in the process of azo dye decolorization and fnally, reused as recycled BSG
for the second successful laccase immobilization for six guaiacol oxidation, contributing to a zero-waste strategy. The novel
fungal strain produced laccase with a maximum activity of 171.4 U/g after 6 days of solid-state fermentation using BSG
as a substrate. The obtained laccase exhibited excellent performance in the decolorization of azo dyes, both as a free and
immobilized, at high temperatures, without addition of harmful mediators, achieving maximum decolorization efciencies
of 99.0%, 71.2%, and 61.0% for Orange G (OG), Congo Red, and Eriochrome Black T (EBT), respectively. The immobilized
laccase on BSG was successfully reused across fve cycles of azo dye decolorization process. Notably, new green biocatalyst
outperformed commercial laccase from Aspergillus spp. in the decolorization of OG and EBT. GC-MS and LC-MS revealed
azo-dye degradation products and decomposition pathway. This analysis was complemented by antimicrobial and phytotoxicity tests, which confrmed the non-toxic nature of the degradation products, indicating the potential for safe environmental
disposal.",
publisher = "Springer",
journal = "Environmental Science and Pollution Research",
title = "Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain",
doi = "10.1007/s11356-024-33367-x"
}

Ilić, N., Davidović, S., Milić, M., Lađarević, J., Onjia, A., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2024). Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain. in Environmental Science and Pollution Research
Springer..
https://doi.org/10.1007/s11356-024-33367-x

Ilić N, Davidović S, Milić M, Lađarević J, Onjia A, Dimitrijević-Branković S, Mihajlovski K. Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain. in Environmental Science and Pollution Research. 2024;.
doi:10.1007/s11356-024-33367-x .

Ilić, Nevena, Davidović, Slađana, Milić, Marija, Lađarević, Jelena, Onjia, Antonije, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain" in Environmental Science and Pollution Research (2024),
https://doi.org/10.1007/s11356-024-33367-x . .

TY  - JOUR
AU  - Milić, Marija
AU  - Buntić, Aneta V.
AU  - Mihajlovski, Katarina
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4981
AB  - This study demonstrated the innovative biotransformation aspect of spent coffee grounds (SCG) by application of the specific enzymatic cocktail with addition of lactic acid bacteria strain Lactobacillus rhamnosus (ATCC (R) 7469 (TM)). This bioprocess induced the improvement of the most of the quality parameters in SCG, causing a considerable increase in the bioactive contents (total polyphenols, chlorogenic acid, reducing sugars, free amino acids) and antioxidant activity, in comparison to the non-fermented SCG. Under the optimal conditions, at 37 degrees C, for 24 h, with 5% v/w of the inoculum employed, the polyphenols were increased for 67% (227.3 +/- 3.3 mg GAE/g dm extract), reducing sugars 57% (277.9 +/- 7.6 mg glucose/g dm extract), and alpha-amino nitrogen 80% (161.2 +/- 9.8 mg/100 g dm extract), and the chlorogenic acid content was doubled, while the caffeine was reduced for 38%. Furthermore, the resulting product evinced a strong antioxidant activity detected via inhibition of DPPH radicals and reducing capacity assay (FRAP). The proposed technology is viable for a large-scale commercialization for utilization of easily accessible industrial residues, deriving a product with a nutritive formulation very desirable to be incorporated as an additive in food and feed industry.
T2  - Biomass Conversion and Biorefinery
T1  - The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization
EP  - 6759
IS  - 8
SP  - 6747
VL  - 13
DO  - 10.1007/s13399-021-01605-8
ER  - 

@article{
author = "Milić, Marija and Buntić, Aneta V. and Mihajlovski, Katarina and Ilić, Nevena and Davidović, Slađana and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "This study demonstrated the innovative biotransformation aspect of spent coffee grounds (SCG) by application of the specific enzymatic cocktail with addition of lactic acid bacteria strain Lactobacillus rhamnosus (ATCC (R) 7469 (TM)). This bioprocess induced the improvement of the most of the quality parameters in SCG, causing a considerable increase in the bioactive contents (total polyphenols, chlorogenic acid, reducing sugars, free amino acids) and antioxidant activity, in comparison to the non-fermented SCG. Under the optimal conditions, at 37 degrees C, for 24 h, with 5% v/w of the inoculum employed, the polyphenols were increased for 67% (227.3 +/- 3.3 mg GAE/g dm extract), reducing sugars 57% (277.9 +/- 7.6 mg glucose/g dm extract), and alpha-amino nitrogen 80% (161.2 +/- 9.8 mg/100 g dm extract), and the chlorogenic acid content was doubled, while the caffeine was reduced for 38%. Furthermore, the resulting product evinced a strong antioxidant activity detected via inhibition of DPPH radicals and reducing capacity assay (FRAP). The proposed technology is viable for a large-scale commercialization for utilization of easily accessible industrial residues, deriving a product with a nutritive formulation very desirable to be incorporated as an additive in food and feed industry.",
journal = "Biomass Conversion and Biorefinery",
title = "The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization",
pages = "6759-6747",
number = "8",
volume = "13",
doi = "10.1007/s13399-021-01605-8"
}

Milić, M., Buntić, A. V., Mihajlovski, K., Ilić, N., Davidović, S.,& Dimitrijević-Branković, S.. (2023). The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization. in Biomass Conversion and Biorefinery, 13(8), 6747-6759.
https://doi.org/10.1007/s13399-021-01605-8

Milić M, Buntić AV, Mihajlovski K, Ilić N, Davidović S, Dimitrijević-Branković S. The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization. in Biomass Conversion and Biorefinery. 2023;13(8):6747-6759.
doi:10.1007/s13399-021-01605-8 .

Milić, Marija, Buntić, Aneta V., Mihajlovski, Katarina, Ilić, Nevena, Davidović, Slađana, Dimitrijević-Branković, Suzana, "The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization" in Biomass Conversion and Biorefinery, 13, no. 8 (2023):6747-6759,
https://doi.org/10.1007/s13399-021-01605-8 . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6605
AB  - Industrial dye effluents that contain triphenylmethane dyes are environment-threatening problem. The triphenylmethane dyes are one of the largest dye’s group that is discharged in large quantities to water bodies. Removing of them with fungal enzymes is big scientific challenge. The fungal laccases are promising tool for removing of these dyes from water bodies. In this study, the potential of crude fungal laccase from genus Ganoderma in decolorization of triphenylmethane dye, crystal violet was investigated. The crude fungal enzyme was produced using agroindustrial lignocellulosic waste, cereal mix. The effect of different substrate masses (15g, 25g and 50 g) and Cu2+ ions concentrations (0.25 mM, 0.75 mM, 1.0 mM, 2.5 mM и 5.0 mM) on lccase production were investigated. The obtained crude fungal laccase with the highest activity was used for decolorization of crystal violet at different concentrations from 20 mg/L to 50 mg/L. The crude fungal laccase from genus Ganoderma had the highest activity of 47.43 U/g, when the substrate mass of 50 g was used for enyzme production. The lower masses of substrate (15g and 25g) induced lower laccase activities of 45.07 U/g and 46.69 U/g, respectively. The initial addition of Cu2+ ions in concentration of 0.25 mM to agroindustrial waste led to decrease of laccase activity compared to the laccase activity of laccase obtained from agroindustrial waste that didn’t contain Cu2+ ions. On the other hand, the increase of Cu2+ concentration to 1.0 mM in the substrate led to increase the laccase activity. The highest laccase activity of 47.53 U/g was obtained when  the 1.0 mM Cu2+ was added to substrate, while the decrease in laccase activity was observed when 5.0 mM Cu2+ was added to the agroindustrial waste. Decolorization of crystal violet at different concentrations (20 mg/L, 30 mg/L, 40 mg/L, 50 mg/L) was carred out at pH 5 and temperature of 50 ºC for 120 min. The highest decolorization efficiency of 14.42 % was obtained in the case of the lowest dye concentration (20 mg/L), while the lowest decolorization efficiency of 3.76 % was obtained when the highest dye concentration of 50 mg/L was decolorized with crude fungal laccase for 120 min. The obtained results show that fungal crude laccases can be used for decolorization of triphenylmethane dyes, but the detailed optimization is very important for obtaining relatively high decolorization efficiencies for short time.
PB  - Zvornik : University of East Sarajevo Faculty of Technology
C3  - Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23
T1  - APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET
EP  - 186
SP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6605
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2023",
abstract = "Industrial dye effluents that contain triphenylmethane dyes are environment-threatening problem. The triphenylmethane dyes are one of the largest dye’s group that is discharged in large quantities to water bodies. Removing of them with fungal enzymes is big scientific challenge. The fungal laccases are promising tool for removing of these dyes from water bodies. In this study, the potential of crude fungal laccase from genus Ganoderma in decolorization of triphenylmethane dye, crystal violet was investigated. The crude fungal enzyme was produced using agroindustrial lignocellulosic waste, cereal mix. The effect of different substrate masses (15g, 25g and 50 g) and Cu2+ ions concentrations (0.25 mM, 0.75 mM, 1.0 mM, 2.5 mM и 5.0 mM) on lccase production were investigated. The obtained crude fungal laccase with the highest activity was used for decolorization of crystal violet at different concentrations from 20 mg/L to 50 mg/L. The crude fungal laccase from genus Ganoderma had the highest activity of 47.43 U/g, when the substrate mass of 50 g was used for enyzme production. The lower masses of substrate (15g and 25g) induced lower laccase activities of 45.07 U/g and 46.69 U/g, respectively. The initial addition of Cu2+ ions in concentration of 0.25 mM to agroindustrial waste led to decrease of laccase activity compared to the laccase activity of laccase obtained from agroindustrial waste that didn’t contain Cu2+ ions. On the other hand, the increase of Cu2+ concentration to 1.0 mM in the substrate led to increase the laccase activity. The highest laccase activity of 47.53 U/g was obtained when  the 1.0 mM Cu2+ was added to substrate, while the decrease in laccase activity was observed when 5.0 mM Cu2+ was added to the agroindustrial waste. Decolorization of crystal violet at different concentrations (20 mg/L, 30 mg/L, 40 mg/L, 50 mg/L) was carred out at pH 5 and temperature of 50 ºC for 120 min. The highest decolorization efficiency of 14.42 % was obtained in the case of the lowest dye concentration (20 mg/L), while the lowest decolorization efficiency of 3.76 % was obtained when the highest dye concentration of 50 mg/L was decolorized with crude fungal laccase for 120 min. The obtained results show that fungal crude laccases can be used for decolorization of triphenylmethane dyes, but the detailed optimization is very important for obtaining relatively high decolorization efficiencies for short time.",
publisher = "Zvornik : University of East Sarajevo Faculty of Technology",
journal = "Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23",
title = "APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET",
pages = "186-179",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6605"
}

Ilić, N., Milić, M., Davidović, S., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2023). APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23
Zvornik : University of East Sarajevo Faculty of Technology., 179-186.
https://hdl.handle.net/21.15107/rcub_technorep_6605

Ilić N, Milić M, Davidović S, Dimitrijević-Branković S, Mihajlovski K. APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23. 2023;:179-186.
https://hdl.handle.net/21.15107/rcub_technorep_6605 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET" in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23 (2023):179-186,
https://hdl.handle.net/21.15107/rcub_technorep_6605 .

TY  - CONF
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Radovanović, Neda
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6943
AB  - In recent decades, enzyme immobilization on different supports occupies an important place in the modern biotechnology, given that it allows the design of green and sustainable production processes. Nanoparticles are very efficient supports for enzyme immobilization. The enzyme immobilized on nanoparticles is more stable than its soluble form and can be used in several operative cycles. In this study, the potential of modified titanium dioxide nanoparticles (Degussa P25, TiO2) for crude fungal laccase (47.43 U/g) immobilization was investigated. The TiO2 nanoparticles were modified with (3-glycidyloxypropyl) trimetoxylane under different conditions in order to obtain support with an optimal concentration of epoxy groups for immobilization. The obtained TiO2 nanoparticles had different concentrations of epoxy groups on their surface, exactly 950 μmol/g (TiO2M1) and 500 μmol/g (TiO2M2), respectively. The immobilization was carried out at pH 5 and at room temperature for 4 h. The residual activity of immobilized laccase on TiO2M2 was 33.40 %, while the residual activity of immobilized laccase on TiO2M1 was 17.39 %. The immobilization efficiency was 26.75 % and 20.18 % for laccase immobilized on TiO2M2 and immobilized laccase on TiO2M1, respectively. The immobilization of crude fungal laccase on TiO2M2 was further optimized by testing the influence of different contact time between laccase and TiO2M2 (2 h ,3 h, 3,5 h and 4 h) as well as the effect of various pH values (pH 4, pH 5 and pH 6) on immobilization. The highest residual activity of 35.04 % and immobilization efficiency of 28.95 % were obtained for optimal contact time of 3.5 h between crude fungal laccase and TiO2M2. The lowest residual activity (30.38 %) and immobilization efficiency (20.18 %) were obtained when immobilization was carried out at room temperature and at pH 5 for 2 h. The pH values had significant effect on immobilization. The optimal pH value was pH 5 with highest residual activity of 35.04 %, while the lowest residual activity of 14. 83 % was at pH 6. The residual activity of 21.21 % and immobilization efficency of 19.52 % were obtained when crude fungal laccase immobilized at pH 4. The immobilized laccase on TiO2M2 was successfully used in 5 cycles of guaiacol oxidation. In the 2nd cycle, the immobilized laccase had residual activity of about 60%, while after 3rd cycle, immobilized laccase had residual activity of 40 %. The lowest residual activity of 14.83 %, immobilized laccase had after 5th cycle.
PB  - Zvornik : University of East Sarajevo Faculty of Technology
C3  - Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023
T1  - IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES
EP  - 196
SP  - 187
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6943
ER  - 

@conference{
author = "Ilić, Nevena and Davidović, Slađana and Miljković, Miona and Radovanović, Neda and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2023",
abstract = "In recent decades, enzyme immobilization on different supports occupies an important place in the modern biotechnology, given that it allows the design of green and sustainable production processes. Nanoparticles are very efficient supports for enzyme immobilization. The enzyme immobilized on nanoparticles is more stable than its soluble form and can be used in several operative cycles. In this study, the potential of modified titanium dioxide nanoparticles (Degussa P25, TiO2) for crude fungal laccase (47.43 U/g) immobilization was investigated. The TiO2 nanoparticles were modified with (3-glycidyloxypropyl) trimetoxylane under different conditions in order to obtain support with an optimal concentration of epoxy groups for immobilization. The obtained TiO2 nanoparticles had different concentrations of epoxy groups on their surface, exactly 950 μmol/g (TiO2M1) and 500 μmol/g (TiO2M2), respectively. The immobilization was carried out at pH 5 and at room temperature for 4 h. The residual activity of immobilized laccase on TiO2M2 was 33.40 %, while the residual activity of immobilized laccase on TiO2M1 was 17.39 %. The immobilization efficiency was 26.75 % and 20.18 % for laccase immobilized on TiO2M2 and immobilized laccase on TiO2M1, respectively. The immobilization of crude fungal laccase on TiO2M2 was further optimized by testing the influence of different contact time between laccase and TiO2M2 (2 h ,3 h, 3,5 h and 4 h) as well as the effect of various pH values (pH 4, pH 5 and pH 6) on immobilization. The highest residual activity of 35.04 % and immobilization efficiency of 28.95 % were obtained for optimal contact time of 3.5 h between crude fungal laccase and TiO2M2. The lowest residual activity (30.38 %) and immobilization efficiency (20.18 %) were obtained when immobilization was carried out at room temperature and at pH 5 for 2 h. The pH values had significant effect on immobilization. The optimal pH value was pH 5 with highest residual activity of 35.04 %, while the lowest residual activity of 14. 83 % was at pH 6. The residual activity of 21.21 % and immobilization efficency of 19.52 % were obtained when crude fungal laccase immobilized at pH 4. The immobilized laccase on TiO2M2 was successfully used in 5 cycles of guaiacol oxidation. In the 2nd cycle, the immobilized laccase had residual activity of about 60%, while after 3rd cycle, immobilized laccase had residual activity of 40 %. The lowest residual activity of 14.83 %, immobilized laccase had after 5th cycle.",
publisher = "Zvornik : University of East Sarajevo Faculty of Technology",
journal = "Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023",
title = "IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES",
pages = "196-187",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6943"
}

Ilić, N., Davidović, S., Miljković, M., Radovanović, N., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2023). IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023
Zvornik : University of East Sarajevo Faculty of Technology., 187-196.
https://hdl.handle.net/21.15107/rcub_technorep_6943

Ilić N, Davidović S, Miljković M, Radovanović N, Dimitrijević-Branković S, Mihajlovski K. IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023. 2023;:187-196.
https://hdl.handle.net/21.15107/rcub_technorep_6943 .

Ilić, Nevena, Davidović, Slađana, Miljković, Miona, Radovanović, Neda, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES" in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023 (2023):187-196,
https://hdl.handle.net/21.15107/rcub_technorep_6943 .

TY  - CONF
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7021
AB  - The use of edible films and coatings made of natural materials such as
polysaccharides provides a sustainable technological alternative to traditional plastic food
packaging materials as one of the contemporary methods for preserving food items and
guaranteeing their quality and freshness throughout their shelf lives. This is especially
important for fresh fruits as highly perishable.
The aim of the present study was to investigate the effect of dextran-based coating on
strawberry (Fragaria × ananassa) quality attributes during refrigerated storage. Dextran was
synthesized using dextransucrase from Leuconostoc mesenteroides T3. Coating solution was
made by dissolving 12g of dextran in 300 mL distilled water. Glycerol (25% w/w) and Tween
80 (5% w/w) were added as plasticizer and surfactant, respectively. Strawberries were coated
by dipping in coating solution or water (control) and after drying were placed in plastic trays
and stored at 8 °C. The influence of coating on strawberry quality was assessed through
measuring the percentage of weight loss (WL), total soluble solids (TSS) and titratable
acidity (TA) of the coated fruits and compared with the control uncoated samples.
The percentage of WL increased during storage in both coated and uncoated
strawberries and although it was less for the uncoated strawberries, the difference was not
significant. The TSS content was lower in dextran-coated fruit during the entire storage
period. Since TSS is an indicator of the ripeness of the fruit it could be concluded that dextran
coating slows down the ripening, thus increasing the shelf life. Unexpectedly, coated
strawberries exhibited lower TA values. However, the relative changes were significantly
smaller among the coated samples group, implying that the coating slows down the
respiration process in which the acids are consumed.
Overall, the results of this work show that the dextran coating has the potential to
preserve quality of strawberries during cold storage.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7021
ER  - 

@conference{
author = "Davidović, Slađana and Miljković, Miona and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "The use of edible films and coatings made of natural materials such as
polysaccharides provides a sustainable technological alternative to traditional plastic food
packaging materials as one of the contemporary methods for preserving food items and
guaranteeing their quality and freshness throughout their shelf lives. This is especially
important for fresh fruits as highly perishable.
The aim of the present study was to investigate the effect of dextran-based coating on
strawberry (Fragaria × ananassa) quality attributes during refrigerated storage. Dextran was
synthesized using dextransucrase from Leuconostoc mesenteroides T3. Coating solution was
made by dissolving 12g of dextran in 300 mL distilled water. Glycerol (25% w/w) and Tween
80 (5% w/w) were added as plasticizer and surfactant, respectively. Strawberries were coated
by dipping in coating solution or water (control) and after drying were placed in plastic trays
and stored at 8 °C. The influence of coating on strawberry quality was assessed through
measuring the percentage of weight loss (WL), total soluble solids (TSS) and titratable
acidity (TA) of the coated fruits and compared with the control uncoated samples.
The percentage of WL increased during storage in both coated and uncoated
strawberries and although it was less for the uncoated strawberries, the difference was not
significant. The TSS content was lower in dextran-coated fruit during the entire storage
period. Since TSS is an indicator of the ripeness of the fruit it could be concluded that dextran
coating slows down the ripening, thus increasing the shelf life. Unexpectedly, coated
strawberries exhibited lower TA values. However, the relative changes were significantly
smaller among the coated samples group, implying that the coating slows down the
respiration process in which the acids are consumed.
Overall, the results of this work show that the dextran coating has the potential to
preserve quality of strawberries during cold storage.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE",
pages = "83",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7021"
}

Davidović, S., Miljković, M.,& Dimitrijević-Branković, S.. (2023). EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 83.
https://hdl.handle.net/21.15107/rcub_technorep_7021

Davidović S, Miljković M, Dimitrijević-Branković S. EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:83.
https://hdl.handle.net/21.15107/rcub_technorep_7021 .

Davidović, Slađana, Miljković, Miona, Dimitrijević-Branković, Suzana, "EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):83,
https://hdl.handle.net/21.15107/rcub_technorep_7021 .

TY  - CONF
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Ilić, Nevena
AU  - Milošević, Jelena
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7030
AB  - By-products of soybean oil production are high-quality protein supplements for
animal feed. However, they are rich in anti-nutritive factors and indigestible components,
among which the special attention is focused on galactooligosaccharides, due to lack of αgalactosidase in monogastric animals. Enzymatic treatment of soy-based products and
microbial fermentation are employed for overcoming these issues.
The main goal of this study was to apply fermentation with a selected strain of black
yeast-like fungus (Aureobasidium spp.) of the soybean products in order to obtain highprotein soy-based animal feed. First, screening for an appropriate strain of microorganism
among ten natural isolates from grapes has been performed. Keeping in mind complex
structure of soybean polysaccharides the first selection criterion was the ability of growth in
the presence of arabinose, xylose, galactose, mannose, raffinose, or soybean oil as sole
carbon source. More important, enzymatic activity of α-galactosidase was detected in new
isolates. The highest α-galactosidase producer was selected for cultivation on soybean meal.
The selected strain was characterized in terms of physiological and technological properties
and identified as Aureobasidium pullulans P8. In order to obtain high protein soybean meal, it
has been utilized in two types of fermentation, i.e. solid state (SSF) and submerged (SMF)
under varying conditions. Maximal protein content (61.11% based on dry weight) was
obtained after 5 days of SMF at 30 °C and 10% of dry matter, while SSF produced 57.78%
protein after 7 days of incubation at 30 °C with substrate contained 30% of dry matter.
Extracellular enzymatic activities of cellulase, pectinase, amylase, xylanase and αgalactosidase were detected in supernatant after SMF, indicating its potential reusability for
hydrolysis of new batch of soybean substrate.
This investigation revealed the versatile extracellular enzymatic potential of newly
isolated black yeast-like fungus Aureobasidium pullulans P8 and its potential for production
of high protein soybean meal.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023
T1  - VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7030
ER  - 

@conference{
author = "Davidović, Slađana and Miljković, Miona and Ilić, Nevena and Milošević, Jelena and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "By-products of soybean oil production are high-quality protein supplements for
animal feed. However, they are rich in anti-nutritive factors and indigestible components,
among which the special attention is focused on galactooligosaccharides, due to lack of αgalactosidase in monogastric animals. Enzymatic treatment of soy-based products and
microbial fermentation are employed for overcoming these issues.
The main goal of this study was to apply fermentation with a selected strain of black
yeast-like fungus (Aureobasidium spp.) of the soybean products in order to obtain highprotein soy-based animal feed. First, screening for an appropriate strain of microorganism
among ten natural isolates from grapes has been performed. Keeping in mind complex
structure of soybean polysaccharides the first selection criterion was the ability of growth in
the presence of arabinose, xylose, galactose, mannose, raffinose, or soybean oil as sole
carbon source. More important, enzymatic activity of α-galactosidase was detected in new
isolates. The highest α-galactosidase producer was selected for cultivation on soybean meal.
The selected strain was characterized in terms of physiological and technological properties
and identified as Aureobasidium pullulans P8. In order to obtain high protein soybean meal, it
has been utilized in two types of fermentation, i.e. solid state (SSF) and submerged (SMF)
under varying conditions. Maximal protein content (61.11% based on dry weight) was
obtained after 5 days of SMF at 30 °C and 10% of dry matter, while SSF produced 57.78%
protein after 7 days of incubation at 30 °C with substrate contained 30% of dry matter.
Extracellular enzymatic activities of cellulase, pectinase, amylase, xylanase and αgalactosidase were detected in supernatant after SMF, indicating its potential reusability for
hydrolysis of new batch of soybean substrate.
This investigation revealed the versatile extracellular enzymatic potential of newly
isolated black yeast-like fungus Aureobasidium pullulans P8 and its potential for production
of high protein soybean meal.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023",
title = "VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS",
pages = "84",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7030"
}

Davidović, S., Miljković, M., Ilić, N., Milošević, J.,& Dimitrijević-Branković, S.. (2023). VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023
Belgrade : University, Faculty of Technology and Metallurgy., 84.
https://hdl.handle.net/21.15107/rcub_technorep_7030

Davidović S, Miljković M, Ilić N, Milošević J, Dimitrijević-Branković S. VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023. 2023;:84.
https://hdl.handle.net/21.15107/rcub_technorep_7030 .

Davidović, Slađana, Miljković, Miona, Ilić, Nevena, Milošević, Jelena, Dimitrijević-Branković, Suzana, "VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023 (2023):84,
https://hdl.handle.net/21.15107/rcub_technorep_7030 .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Meseldžija, Slađana
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Onjia, Antonije
AU  - Dimitrijević, Suzana
AU  - Santagata, Gabriella
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6613
AB  - In this study, pectin-based films blended with alginate and crosslinked with copper ions for potential application in agriculture were investigated. Copper was selected as crosslinking agent, due to its key role as a plant micronutrient, antifungal ability, and focal activity in the pectin crosslinking process. FTIR analysis confirmed the interactions between pectin, alginate and copper salt. The inclusion of alginate in film improved the binding activity of copper ions, providing denser 3d network with enhanced physical-chemical properties. The crosslinked film containing 60 wt% of pectin and 40 wt% of alginate had the highest tensile strength (59.8 MPa), highest UV barrier (transmission of light=0%), and the lowest swelling degree (121%) and water vapor permeability (0.320 ng/ m s Pa). Moreover, the release of copper ions from this formulation after 28 days of immersion in water and soil was 9 and 13 ppm, respectively. The biodegradation rate after 28 days of exposure in the soil was 54%. Nevertheless, all crosslinked pectin-alginate films inhibited the growth of the Fusarium and Aspergillus phytopathogens and demonstrated non-phytotoxicity on germination and growth of wheat. Hence, the system developed in this work can potentially be used as novel, bio-active, tunable plant protective biodegradable foliar spray in agriculture sector.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Characterization of antifungal citrus pectin-based films for potential agricultural application
SP  - 117386
VL  - 204
DO  - 10.1016/j.indcrop.2023.117386
ER  - 

@article{
author = "Nešić, Aleksandra and Meseldžija, Slađana and Davidović, Slađana and Miljković, Miona and Onjia, Antonije and Dimitrijević, Suzana and Santagata, Gabriella",
year = "2023",
abstract = "In this study, pectin-based films blended with alginate and crosslinked with copper ions for potential application in agriculture were investigated. Copper was selected as crosslinking agent, due to its key role as a plant micronutrient, antifungal ability, and focal activity in the pectin crosslinking process. FTIR analysis confirmed the interactions between pectin, alginate and copper salt. The inclusion of alginate in film improved the binding activity of copper ions, providing denser 3d network with enhanced physical-chemical properties. The crosslinked film containing 60 wt% of pectin and 40 wt% of alginate had the highest tensile strength (59.8 MPa), highest UV barrier (transmission of light=0%), and the lowest swelling degree (121%) and water vapor permeability (0.320 ng/ m s Pa). Moreover, the release of copper ions from this formulation after 28 days of immersion in water and soil was 9 and 13 ppm, respectively. The biodegradation rate after 28 days of exposure in the soil was 54%. Nevertheless, all crosslinked pectin-alginate films inhibited the growth of the Fusarium and Aspergillus phytopathogens and demonstrated non-phytotoxicity on germination and growth of wheat. Hence, the system developed in this work can potentially be used as novel, bio-active, tunable plant protective biodegradable foliar spray in agriculture sector.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Characterization of antifungal citrus pectin-based films for potential agricultural application",
pages = "117386",
volume = "204",
doi = "10.1016/j.indcrop.2023.117386"
}

Nešić, A., Meseldžija, S., Davidović, S., Miljković, M., Onjia, A., Dimitrijević, S.,& Santagata, G.. (2023). Characterization of antifungal citrus pectin-based films for potential agricultural application. in Industrial Crops and Products
Elsevier B.V.., 204, 117386.
https://doi.org/10.1016/j.indcrop.2023.117386

Nešić A, Meseldžija S, Davidović S, Miljković M, Onjia A, Dimitrijević S, Santagata G. Characterization of antifungal citrus pectin-based films for potential agricultural application. in Industrial Crops and Products. 2023;204:117386.
doi:10.1016/j.indcrop.2023.117386 .

Nešić, Aleksandra, Meseldžija, Slađana, Davidović, Slađana, Miljković, Miona, Onjia, Antonije, Dimitrijević, Suzana, Santagata, Gabriella, "Characterization of antifungal citrus pectin-based films for potential agricultural application" in Industrial Crops and Products, 204 (2023):117386,
https://doi.org/10.1016/j.indcrop.2023.117386 . .

TY  - JOUR
AU  - Mašojević, Dijana
AU  - Stamenović, Una
AU  - Otoničar, Mojca
AU  - Davidović, Slađana
AU  - Škapin, Srečo
AU  - Barudžija, Tanja
AU  - Vodnik, Vesna
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5918
AB  - A novel and effective antibacterial agent based on a combination of copper nanoparticles (CuNPs) and polypyrrole (PPy) is presented. By using a straightforward in situ polymerization method in water, a copper/polypyrrole (Cu/PPy) nanocomposite with spherical CuNPs, around 25 nm in diameter, uniformly dispersed throughout a granular PPy matrix, was characterized and subjected to determination of antibacterial activity toward E. coli, and S. aureus. In this survey, the synergistic engagement of various features of CuNPs and PPy against bacteria was somewhat expected. After a 24 h interaction with S. aureus, already 2 ppm of Cu/PPy with only 9.45 wt% content of Cu is acting almost completely cidal, with 99.17 % bacterial growth inhibition, while 10 ppm has a cidal value of 99.99 % at the same time. Safe and environmentally acceptable concentrations of Cu/PPy indicate that it is convenient as an antibacterial agent for wider use.
PB  - Elsevier B.V.
T2  - Materials Letters
T1  - Evaluation of the antibacterial effectiveness of novel copper/polypyrrole nanocomposite
SP  - 134051
VL  - 338
DO  - 10.1016/j.matlet.2023.134051
ER  - 

@article{
author = "Mašojević, Dijana and Stamenović, Una and Otoničar, Mojca and Davidović, Slađana and Škapin, Srečo and Barudžija, Tanja and Vodnik, Vesna",
year = "2023",
abstract = "A novel and effective antibacterial agent based on a combination of copper nanoparticles (CuNPs) and polypyrrole (PPy) is presented. By using a straightforward in situ polymerization method in water, a copper/polypyrrole (Cu/PPy) nanocomposite with spherical CuNPs, around 25 nm in diameter, uniformly dispersed throughout a granular PPy matrix, was characterized and subjected to determination of antibacterial activity toward E. coli, and S. aureus. In this survey, the synergistic engagement of various features of CuNPs and PPy against bacteria was somewhat expected. After a 24 h interaction with S. aureus, already 2 ppm of Cu/PPy with only 9.45 wt% content of Cu is acting almost completely cidal, with 99.17 % bacterial growth inhibition, while 10 ppm has a cidal value of 99.99 % at the same time. Safe and environmentally acceptable concentrations of Cu/PPy indicate that it is convenient as an antibacterial agent for wider use.",
publisher = "Elsevier B.V.",
journal = "Materials Letters",
title = "Evaluation of the antibacterial effectiveness of novel copper/polypyrrole nanocomposite",
pages = "134051",
volume = "338",
doi = "10.1016/j.matlet.2023.134051"
}

Mašojević, D., Stamenović, U., Otoničar, M., Davidović, S., Škapin, S., Barudžija, T.,& Vodnik, V.. (2023). Evaluation of the antibacterial effectiveness of novel copper/polypyrrole nanocomposite. in Materials Letters
Elsevier B.V.., 338, 134051.
https://doi.org/10.1016/j.matlet.2023.134051

Mašojević D, Stamenović U, Otoničar M, Davidović S, Škapin S, Barudžija T, Vodnik V. Evaluation of the antibacterial effectiveness of novel copper/polypyrrole nanocomposite. in Materials Letters. 2023;338:134051.
doi:10.1016/j.matlet.2023.134051 .

Mašojević, Dijana, Stamenović, Una, Otoničar, Mojca, Davidović, Slađana, Škapin, Srečo, Barudžija, Tanja, Vodnik, Vesna, "Evaluation of the antibacterial effectiveness of novel copper/polypyrrole nanocomposite" in Materials Letters, 338 (2023):134051,
https://doi.org/10.1016/j.matlet.2023.134051 . .

TY  - JOUR
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Milić, Marija
AU  - Rajilić-Stojanović, Mirjana
AU  - Pecarski, Danijela
AU  - Ivancic-Santek, Mirela
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5001
AB  - This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewer's spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewer 's spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewer's spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.
T2  - Biomass Conversion and Biorefinery
T1  - Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production
EP  - 17186
SP  - 17175
VL  - 13
DO  - 10.1007/s13399-021-02145-x
ER  - 

@article{
author = "Ilić, Nevena and Davidović, Slađana and Milić, Marija and Rajilić-Stojanović, Mirjana and Pecarski, Danijela and Ivancic-Santek, Mirela and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewer's spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewer 's spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewer's spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.",
journal = "Biomass Conversion and Biorefinery",
title = "Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production",
pages = "17186-17175",
volume = "13",
doi = "10.1007/s13399-021-02145-x"
}

Ilić, N., Davidović, S., Milić, M., Rajilić-Stojanović, M., Pecarski, D., Ivancic-Santek, M., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2022). Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. in Biomass Conversion and Biorefinery, 13, 17175-17186.
https://doi.org/10.1007/s13399-021-02145-x

Ilić N, Davidović S, Milić M, Rajilić-Stojanović M, Pecarski D, Ivancic-Santek M, Mihajlovski K, Dimitrijević-Branković S. Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. in Biomass Conversion and Biorefinery. 2022;13:17175-17186.
doi:10.1007/s13399-021-02145-x .

Ilić, Nevena, Davidović, Slađana, Milić, Marija, Rajilić-Stojanović, Mirjana, Pecarski, Danijela, Ivancic-Santek, Mirela, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production" in Biomass Conversion and Biorefinery, 13 (2022):17175-17186,
https://doi.org/10.1007/s13399-021-02145-x . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Kostić, Anđela
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5940
AB  - In recent years, one of the biggest environmental problems is the pollution of water with colored wastewater which has negative effect on the environment and human health. Wastewaters contain complex structural compounds, such as azo dyes that used today in many industrial fields. Removing of azo dyes from wastewater using traditional methods is an extremely complex and in many cases ineffective process. In recent decades, there is a tendency towards the application of environmentally acceptable methods of removing synthetic dyes from wastewater. Method which has proven to be very effective, is the degradation of synthetic dyes using various fungal enzymes. In this study, the crude fungal laccase (31,42 UmL-1) obtained by growing fungal mycelium Ganoderma spp. on cereal mix was used for decolorization of Methyl Orange. Decolorization procedure was carried out at different temperatures (30-70 ºC) and pH (3-8) in order to determine the optimal conditions for dye decolorization. The incubation time was 180 min and every 15 min during the incubation time, the change in color intensity was monitored spectrophotometrically at 472 nm and decolorization efficiency (DE) was calculated. The optimal pH was 5 with DE of 57 % at 30 ºC, while in the case of other pH values, DE was lower. The lowest DE (1,2 %) was in the case of pH 8, which indicates that laccase activity decreases in the alkaline medium. The optimal temperature of decolorization was 50 ºC with DE of 62 % at pH 5, while the DE was lower at higher and lower temperatures, which is in accordance with the literature data on the laccase activity optimal temperature of the Ganoderma spp. The lowest DE was 35 % at 70 ºC and pH 5. The obtained results show that laccase with good decolorization properties can be obtained using cheap agro-industrial wastes, such as cereal mix. The low cost of laccase production as well as the relatively high DE in a short time may further broaden its application in wastewater treatment.
C3  - Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,
T1  - Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5940
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Kostić, Anđela and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "In recent years, one of the biggest environmental problems is the pollution of water with colored wastewater which has negative effect on the environment and human health. Wastewaters contain complex structural compounds, such as azo dyes that used today in many industrial fields. Removing of azo dyes from wastewater using traditional methods is an extremely complex and in many cases ineffective process. In recent decades, there is a tendency towards the application of environmentally acceptable methods of removing synthetic dyes from wastewater. Method which has proven to be very effective, is the degradation of synthetic dyes using various fungal enzymes. In this study, the crude fungal laccase (31,42 UmL-1) obtained by growing fungal mycelium Ganoderma spp. on cereal mix was used for decolorization of Methyl Orange. Decolorization procedure was carried out at different temperatures (30-70 ºC) and pH (3-8) in order to determine the optimal conditions for dye decolorization. The incubation time was 180 min and every 15 min during the incubation time, the change in color intensity was monitored spectrophotometrically at 472 nm and decolorization efficiency (DE) was calculated. The optimal pH was 5 with DE of 57 % at 30 ºC, while in the case of other pH values, DE was lower. The lowest DE (1,2 %) was in the case of pH 8, which indicates that laccase activity decreases in the alkaline medium. The optimal temperature of decolorization was 50 ºC with DE of 62 % at pH 5, while the DE was lower at higher and lower temperatures, which is in accordance with the literature data on the laccase activity optimal temperature of the Ganoderma spp. The lowest DE was 35 % at 70 ºC and pH 5. The obtained results show that laccase with good decolorization properties can be obtained using cheap agro-industrial wastes, such as cereal mix. The low cost of laccase production as well as the relatively high DE in a short time may further broaden its application in wastewater treatment.",
journal = "Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,",
title = "Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5940"
}

Ilić, N., Milić, M., Davidović, S., Kostić, A., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2022). Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix. in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,, 80.
https://hdl.handle.net/21.15107/rcub_technorep_5940

Ilić N, Milić M, Davidović S, Kostić A, Mihajlovski K, Dimitrijević-Branković S. Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix. in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,. 2022;:80.
https://hdl.handle.net/21.15107/rcub_technorep_5940 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Kostić, Anđela, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix" in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering, (2022):80,
https://hdl.handle.net/21.15107/rcub_technorep_5940 .

TY  - CONF
AU  - Mašojević, Dijana
AU  - Vodnik, Vesna
AU  - Spasojević, Jelena
AU  - Barudžija, Tanja
AU  - Otoničar, Mojca
AU  - Davidović, Slađana
AU  - Stamenović, Una
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6124
AB  - A novel and favorable antibacterial agent as a combination of copper nanoparticles (CuNPs) and polypyrrole (PPy) is presented. By simple in situ polymerization method, copper/polypyrrole (Cu/PPy) nanocomposite with spherical CuNPs, around 25 nm in diameter, uniformly dispersed throughout PPy matrix with granular morphology, was characterized and subjected to determination of antibacterial activity toward bacteria Escherichia coli and Staphylococcus aureus. Knowing that when acting alone, CuNPs and PPy have already shown antimicrobial responce, their mutual and synergetic engagement against bacteria in this survey is somewhat expected. After a twenty-four-hour interaction with S. aureus, already 2 ppm of Cu/PPy with only 9.45 wt% content of Cu is acting almost completely cidal, with 99.17% of bacterial growth inhibition. Such nanocomposite could easily find application in water disinfection, soil sterilization and food preservation.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022 16 th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - ANTIBACTERIAL ACTIVITY OF COPPER/POLYPYRROLE NANOCOMPOSITE
SP  - 174
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6124
ER  - 

@conference{
author = "Mašojević, Dijana and Vodnik, Vesna and Spasojević, Jelena and Barudžija, Tanja and Otoničar, Mojca and Davidović, Slađana and Stamenović, Una",
year = "2022",
abstract = "A novel and favorable antibacterial agent as a combination of copper nanoparticles (CuNPs) and polypyrrole (PPy) is presented. By simple in situ polymerization method, copper/polypyrrole (Cu/PPy) nanocomposite with spherical CuNPs, around 25 nm in diameter, uniformly dispersed throughout PPy matrix with granular morphology, was characterized and subjected to determination of antibacterial activity toward bacteria Escherichia coli and Staphylococcus aureus. Knowing that when acting alone, CuNPs and PPy have already shown antimicrobial responce, their mutual and synergetic engagement against bacteria in this survey is somewhat expected. After a twenty-four-hour interaction with S. aureus, already 2 ppm of Cu/PPy with only 9.45 wt% content of Cu is acting almost completely cidal, with 99.17% of bacterial growth inhibition. Such nanocomposite could easily find application in water disinfection, soil sterilization and food preservation.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022 16 th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "ANTIBACTERIAL ACTIVITY OF COPPER/POLYPYRROLE NANOCOMPOSITE",
pages = "174",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6124"
}

Mašojević, D., Vodnik, V., Spasojević, J., Barudžija, T., Otoničar, M., Davidović, S.,& Stamenović, U.. (2022). ANTIBACTERIAL ACTIVITY OF COPPER/POLYPYRROLE NANOCOMPOSITE. in PHYSICAL CHEMISTRY 2022 16 th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Belgrade : Society of Physical Chemists of Serbia., 174.
https://hdl.handle.net/21.15107/rcub_technorep_6124

Mašojević D, Vodnik V, Spasojević J, Barudžija T, Otoničar M, Davidović S, Stamenović U. ANTIBACTERIAL ACTIVITY OF COPPER/POLYPYRROLE NANOCOMPOSITE. in PHYSICAL CHEMISTRY 2022 16 th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2022;:174.
https://hdl.handle.net/21.15107/rcub_technorep_6124 .

Mašojević, Dijana, Vodnik, Vesna, Spasojević, Jelena, Barudžija, Tanja, Otoničar, Mojca, Davidović, Slađana, Stamenović, Una, "ANTIBACTERIAL ACTIVITY OF COPPER/POLYPYRROLE NANOCOMPOSITE" in PHYSICAL CHEMISTRY 2022 16 th International Conference on Fundamental and Applied Aspects of Physical Chemistry (2022):174,
https://hdl.handle.net/21.15107/rcub_technorep_6124 .

TY  - CONF
AU  - Ilić, Nevena
AU  - Kukučka, Andrej
AU  - Milić, Marija
AU  - Milutinović, Milica
AU  - Miljković, Miona
AU  - Davidović, Slađana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5938
AB  - Antimicrobial properties of silver nanoparticles (Ag NPs) are well known and widely exploited. Various approaches have been applied for preparation of Ag NPs. Nowadays, „green“ methods are attracting more attention since they are eco-friendly and generally cheap. Moreover, incorporation of Ag NPs into various natural polymers enables preparation of biocompatible antimicrobial materials with controlled releasing of Ag NPs. Polymeric material also provides long term stability of Ag NPs. The aim of this study was to develop antimicrobial materials based on silver nanoparticles using green approach. Reduction of silver was performed by plant extracts of horsetail (Equisetum arvense L.) and cocoa (Theobroma cacao); the obtained nanoparticles were designated as EA Ag NPs and TC Ag NPs, respectively. The effect of extract concentration on particle size distribution and antimicrobial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus were investigated. The smallest particles with the narrowest size distribution (between 46 nm and 91 nm) were obtained with the lowest tested concentration (10% v/v) of plant extract for both horsetail and cacao. These samples also showed the highest antibacterial activities, so they were selected for preparation of nanocomposite films based on agar-agar and chitosan. It has been shown that incorporation of both EA and TC Ag NPs significantly improved antibacterial properties of the films against E. coli (≈80% higher inhibition in comparison to the pristine agar-agar/chitosan films). In the case of S. aures, TC Ag NPs incorporated in agar-agar/chitosan films enhanced inhibition of the film for 60%. On the other side, incorporation of EA Ag NPs into agar-agar/chitosan film increased the growth inhibition for only 20%. This might be due to the contribution of cocoa extract itself, i.e. some active components that inhibit the growth of S. aureus. Our results show that agar-agar/chitosan films with incorporated green synthesized Ag NPs have potential application in the areas where usage of biodegradable and biocompatible materials with high antibacterial activity is desired, such as food package, wound healing, coatings for medical devices etc.
C3  - Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry
T1  - Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles
EP  - 469
SP  - 461
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5938
ER  - 

@conference{
author = "Ilić, Nevena and Kukučka, Andrej and Milić, Marija and Milutinović, Milica and Miljković, Miona and Davidović, Slađana",
year = "2021",
abstract = "Antimicrobial properties of silver nanoparticles (Ag NPs) are well known and widely exploited. Various approaches have been applied for preparation of Ag NPs. Nowadays, „green“ methods are attracting more attention since they are eco-friendly and generally cheap. Moreover, incorporation of Ag NPs into various natural polymers enables preparation of biocompatible antimicrobial materials with controlled releasing of Ag NPs. Polymeric material also provides long term stability of Ag NPs. The aim of this study was to develop antimicrobial materials based on silver nanoparticles using green approach. Reduction of silver was performed by plant extracts of horsetail (Equisetum arvense L.) and cocoa (Theobroma cacao); the obtained nanoparticles were designated as EA Ag NPs and TC Ag NPs, respectively. The effect of extract concentration on particle size distribution and antimicrobial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus were investigated. The smallest particles with the narrowest size distribution (between 46 nm and 91 nm) were obtained with the lowest tested concentration (10% v/v) of plant extract for both horsetail and cacao. These samples also showed the highest antibacterial activities, so they were selected for preparation of nanocomposite films based on agar-agar and chitosan. It has been shown that incorporation of both EA and TC Ag NPs significantly improved antibacterial properties of the films against E. coli (≈80% higher inhibition in comparison to the pristine agar-agar/chitosan films). In the case of S. aures, TC Ag NPs incorporated in agar-agar/chitosan films enhanced inhibition of the film for 60%. On the other side, incorporation of EA Ag NPs into agar-agar/chitosan film increased the growth inhibition for only 20%. This might be due to the contribution of cocoa extract itself, i.e. some active components that inhibit the growth of S. aureus. Our results show that agar-agar/chitosan films with incorporated green synthesized Ag NPs have potential application in the areas where usage of biodegradable and biocompatible materials with high antibacterial activity is desired, such as food package, wound healing, coatings for medical devices etc.",
journal = "Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry",
title = "Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles",
pages = "469-461",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5938"
}

Ilić, N., Kukučka, A., Milić, M., Milutinović, M., Miljković, M.,& Davidović, S.. (2021). Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry, 461-469.
https://hdl.handle.net/21.15107/rcub_technorep_5938

Ilić N, Kukučka A, Milić M, Milutinović M, Miljković M, Davidović S. Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry. 2021;:461-469.
https://hdl.handle.net/21.15107/rcub_technorep_5938 .

Ilić, Nevena, Kukučka, Andrej, Milić, Marija, Milutinović, Milica, Miljković, Miona, Davidović, Slađana, "Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles" in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry (2021):461-469,
https://hdl.handle.net/21.15107/rcub_technorep_5938 .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5939
AB  - The polymerization and crosslinking of various phenolic compounds induced by the laccase enzyme have been in recent years increasingly used in food industry, due to the emergence of products with improved properties, especially with increased antioxidant activity. In this regard, this study has explored the laccase-induced structural changes of two types of naturally occurring polyphenols, named gallic acid and epigallocatechin gallate, and their antioxidant activity. In the first part, the effect of the laccase of different origin - laccase of white rot fungi (Ganoderma spp.), previously grown on waste cereals and commercial laccase from Novozym® 51003, were investigated. Based on the results obtained after incubation (24 h, 50ºC), the sample containing a mixture of polyphenols, rather than individually, gave visible changes in the reading of the UV-Vis spectrum and increased antioxidant activity with small differences between commercial laccase and laccase of white rot fungi, which indicates that laccase obtained by growing fungi on waste cereals may be competitive for such a purpose. Moreover, the use of such an obtained laccase is more environmentally friendly and economically viable than use of commercial one. Further process optimization of polyphenols polymerization was performed within the method of multifactor statistical analysis (Design Expert), by using of commercial laccase, where by the effect of enzyme concentrations (0.1, 0.3 and 0.5 U/ml) and incubation time (4, 14 and 24 h) were monitored. The maximal antioxidant activity, measured by both DPPH (inhibition of 58.58% of radicals) and FRAP (176.57 mmol Fe2+/ml) was achieved by a sample containing a mixture of polyphenols and 0.3 U/ml laccase enzymes, after 14 h, at 50˚C. The results of this study revealed that careful optimization of process variables during polyphenols polymerization is extremely important for obtaining the product with desirable value added properties, which may be implemented in food and pharmaceutical industry.
C3  - Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021
T1  - The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme
SP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5939
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "The polymerization and crosslinking of various phenolic compounds induced by the laccase enzyme have been in recent years increasingly used in food industry, due to the emergence of products with improved properties, especially with increased antioxidant activity. In this regard, this study has explored the laccase-induced structural changes of two types of naturally occurring polyphenols, named gallic acid and epigallocatechin gallate, and their antioxidant activity. In the first part, the effect of the laccase of different origin - laccase of white rot fungi (Ganoderma spp.), previously grown on waste cereals and commercial laccase from Novozym® 51003, were investigated. Based on the results obtained after incubation (24 h, 50ºC), the sample containing a mixture of polyphenols, rather than individually, gave visible changes in the reading of the UV-Vis spectrum and increased antioxidant activity with small differences between commercial laccase and laccase of white rot fungi, which indicates that laccase obtained by growing fungi on waste cereals may be competitive for such a purpose. Moreover, the use of such an obtained laccase is more environmentally friendly and economically viable than use of commercial one. Further process optimization of polyphenols polymerization was performed within the method of multifactor statistical analysis (Design Expert), by using of commercial laccase, where by the effect of enzyme concentrations (0.1, 0.3 and 0.5 U/ml) and incubation time (4, 14 and 24 h) were monitored. The maximal antioxidant activity, measured by both DPPH (inhibition of 58.58% of radicals) and FRAP (176.57 mmol Fe2+/ml) was achieved by a sample containing a mixture of polyphenols and 0.3 U/ml laccase enzymes, after 14 h, at 50˚C. The results of this study revealed that careful optimization of process variables during polyphenols polymerization is extremely important for obtaining the product with desirable value added properties, which may be implemented in food and pharmaceutical industry.",
journal = "Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021",
title = "The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5939"
}

Ilić, N., Milić, M., Davidović, S., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2021). The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme. in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021, 158.
https://hdl.handle.net/21.15107/rcub_technorep_5939

Ilić N, Milić M, Davidović S, Mihajlovski K, Dimitrijević-Branković S. The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme. in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021. 2021;:158.
https://hdl.handle.net/21.15107/rcub_technorep_5939 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme" in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021 (2021):158,
https://hdl.handle.net/21.15107/rcub_technorep_5939 .

TY  - CONF
AU  - Ilić, Nevena
AU  - Lazić, Vesna
AU  - Radovanović, Neda
AU  - Mihajlovski, Katarina
AU  - Davidović, Slađana
AU  - Miljković, Miona
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5927
AB  - Silver and titanium dioxide nanoparticles (NPs) are the most produced NPs worldwide due to their antimicrobial properties. These NPs are widely used in many fields including agriculture, where they can inhibit the growth of plant pathogens and stimulate plant growth. However, there are concerns about nanoparticles reaching the environment, as they can also manifest adverse effects. In this study, the effects of TiO2 and two types of Ag NPs (synthesized by chemical reduction and “green” by dextrane) on soil microorganisms and organic beans were investigated. Two different species of rhizospheric soil microorganisms, namely actinomycetes Streptomyces microflavus and nitrogen-fixing soil bacteria Rhizobium sp. were exposed to the low concentration of NPs. For that purpose, microorganisms were grown in nutrient media supplemented with 0.2 mM NPs. After 24 h of exposure, the numbers of viable cells of both tested microorganisms were higher in the samples compared to the controls (media without NPs) for all three NPs, suggesting their stimulatory effect on the soil microorganisms' growth. The highest positive effect was observed for Rhizobium sp. treated with TiO2 NPs (the number of viable cells increased for two orders of magnitude, from ~106 to ~108 CFU/mL). Ag NPs showed equal influence on both tested microorganisms, with dextrane-coated Ag NPs were more effective. In the experiment with organic mung beans, the same colloid solutions of Ag NPs and TiO2 NPs had a stimulating effect on the germination and root length of this plant, also. The number of germinated seeds was the same as in the control, while the average root length was higher in the samples than in the control. The average root length was 1.47 cm in the control. Seeds treated with dextrane-coated Ag NPs had an average root length even 82 % higher compared to the control. The stimulatory effect was also observed in the case of seeds treated with Ag NPs synthesized by chemical reduction and TiO2 NPs, where the average root lengths were higher for 40% and 76 %, respectively than in the control. Germination index values, which are higher than 100%, suggesting that tested nanoparticles can be used as fertilizers in agriculture. Moreover, the obtained results show that silver nanoparticles and titanium dioxide nanoparticles have a stimulatory effect on the soil bacteria and organic mung bean, which prove their safety for this kind of application.
C3  - Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry
T1  - Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean
EP  - 460
SP  - 451
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5927
ER  - 

@conference{
author = "Ilić, Nevena and Lazić, Vesna and Radovanović, Neda and Mihajlovski, Katarina and Davidović, Slađana and Miljković, Miona",
year = "2021",
abstract = "Silver and titanium dioxide nanoparticles (NPs) are the most produced NPs worldwide due to their antimicrobial properties. These NPs are widely used in many fields including agriculture, where they can inhibit the growth of plant pathogens and stimulate plant growth. However, there are concerns about nanoparticles reaching the environment, as they can also manifest adverse effects. In this study, the effects of TiO2 and two types of Ag NPs (synthesized by chemical reduction and “green” by dextrane) on soil microorganisms and organic beans were investigated. Two different species of rhizospheric soil microorganisms, namely actinomycetes Streptomyces microflavus and nitrogen-fixing soil bacteria Rhizobium sp. were exposed to the low concentration of NPs. For that purpose, microorganisms were grown in nutrient media supplemented with 0.2 mM NPs. After 24 h of exposure, the numbers of viable cells of both tested microorganisms were higher in the samples compared to the controls (media without NPs) for all three NPs, suggesting their stimulatory effect on the soil microorganisms' growth. The highest positive effect was observed for Rhizobium sp. treated with TiO2 NPs (the number of viable cells increased for two orders of magnitude, from ~106 to ~108 CFU/mL). Ag NPs showed equal influence on both tested microorganisms, with dextrane-coated Ag NPs were more effective. In the experiment with organic mung beans, the same colloid solutions of Ag NPs and TiO2 NPs had a stimulating effect on the germination and root length of this plant, also. The number of germinated seeds was the same as in the control, while the average root length was higher in the samples than in the control. The average root length was 1.47 cm in the control. Seeds treated with dextrane-coated Ag NPs had an average root length even 82 % higher compared to the control. The stimulatory effect was also observed in the case of seeds treated with Ag NPs synthesized by chemical reduction and TiO2 NPs, where the average root lengths were higher for 40% and 76 %, respectively than in the control. Germination index values, which are higher than 100%, suggesting that tested nanoparticles can be used as fertilizers in agriculture. Moreover, the obtained results show that silver nanoparticles and titanium dioxide nanoparticles have a stimulatory effect on the soil bacteria and organic mung bean, which prove their safety for this kind of application.",
journal = "Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry",
title = "Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean",
pages = "460-451",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5927"
}

Ilić, N., Lazić, V., Radovanović, N., Mihajlovski, K., Davidović, S.,& Miljković, M.. (2021). Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry, 451-460.
https://hdl.handle.net/21.15107/rcub_technorep_5927

Ilić N, Lazić V, Radovanović N, Mihajlovski K, Davidović S, Miljković M. Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry. 2021;:451-460.
https://hdl.handle.net/21.15107/rcub_technorep_5927 .

Ilić, Nevena, Lazić, Vesna, Radovanović, Neda, Mihajlovski, Katarina, Davidović, Slađana, Miljković, Miona, "Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean" in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry (2021):451-460,
https://hdl.handle.net/21.15107/rcub_technorep_5927 .

TY  - JOUR
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Đukić-Vuković, Aleksandra
AU  - Ilic, Mila, V
AU  - Simović, Milica
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4791
AB  - Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391 +/- 0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.
T2  - Hemijska industrija
T1  - Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology
EP  - 146
IS  - 3
SP  - 135
VL  - 75
DO  - 10.2298/HEMIND200710015M
ER  - 

@article{
author = "Miljković, Miona and Davidović, Slađana and Đukić-Vuković, Aleksandra and Ilic, Mila, V and Simović, Milica and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391 +/- 0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.",
journal = "Hemijska industrija",
title = "Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology",
pages = "146-135",
number = "3",
volume = "75",
doi = "10.2298/HEMIND200710015M"
}

Miljković, M., Davidović, S., Đukić-Vuković, A., Ilic, M. V., Simović, M., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology. in Hemijska industrija, 75(3), 135-146.
https://doi.org/10.2298/HEMIND200710015M

Miljković M, Davidović S, Đukić-Vuković A, Ilic MV, Simović M, Rajilić-Stojanović M, Dimitrijević-Branković S. Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology. in Hemijska industrija. 2021;75(3):135-146.
doi:10.2298/HEMIND200710015M .

Miljković, Miona, Davidović, Slađana, Đukić-Vuković, Aleksandra, Ilic, Mila, V, Simović, Milica, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology" in Hemijska industrija, 75, no. 3 (2021):135-146,
https://doi.org/10.2298/HEMIND200710015M . .

TY  - JOUR
AU  - Stamenovic, Una
AU  - Davidović, Slađana
AU  - Petrovic, Sandra
AU  - Leskovac, Andreja
AU  - Stoiljkovic, Milovan
AU  - Vodnik, Vesna
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4852
AB  - Two silver-polyaniline/polyvinylpyrrolidone (Ag-PANI/PVP) nanocomposites were prepared using in situ integration of silver nanoparticles (AgNPs) during oxidative aniline polymerization, accelerated by the presence of PVP, which as well minimized the risk of particle agglomeration and macroscopic precipitation. Both nanocomposites have similar silver content (similar to 44 wt% Ag) but different morphological features of AgNPs (spheres/triangles) and polymers (granular/wrinkling pattern). Several spectroscopic, macroscopic, and analytical techniques, microscopy, and surface analysis methods have been used to analyze their physical and chemical properties. Investigation of their antimicrobial potential and possible application as an effective weapon against indicator microbial strains, E. coli, S. aureus, and C. albicans, has shown inhibition of microbial growth by more than 90%, even at low composite concentrations (5 ppm) and short contact time (4 h). A biosafety assessment of Ag-PANI/PVP that comprised testing the genotoxicity and redox modulating activity was performed using human peripheral blood cells as a model system. The obtained results have shown that the investigated Ag-PANI/PVP exhibited significant prooxidant and cytostatic effects (p   lt 0.05) with no apparent potential to induce DNA damage. Although precautions should be taken to protect human health, the significant antimicrobial efficiency of Ag-PANI/PVP makes it suitable for further studies and applications in non-medical areas, such as wastewater treatment.
T2  - New Journal of Chemistry
T1  - Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles
EP  - 12720
IS  - 28
SP  - 12711
VL  - 45
DO  - 10.1039/d1nj02729h
ER  - 

@article{
author = "Stamenovic, Una and Davidović, Slađana and Petrovic, Sandra and Leskovac, Andreja and Stoiljkovic, Milovan and Vodnik, Vesna",
year = "2021",
abstract = "Two silver-polyaniline/polyvinylpyrrolidone (Ag-PANI/PVP) nanocomposites were prepared using in situ integration of silver nanoparticles (AgNPs) during oxidative aniline polymerization, accelerated by the presence of PVP, which as well minimized the risk of particle agglomeration and macroscopic precipitation. Both nanocomposites have similar silver content (similar to 44 wt% Ag) but different morphological features of AgNPs (spheres/triangles) and polymers (granular/wrinkling pattern). Several spectroscopic, macroscopic, and analytical techniques, microscopy, and surface analysis methods have been used to analyze their physical and chemical properties. Investigation of their antimicrobial potential and possible application as an effective weapon against indicator microbial strains, E. coli, S. aureus, and C. albicans, has shown inhibition of microbial growth by more than 90%, even at low composite concentrations (5 ppm) and short contact time (4 h). A biosafety assessment of Ag-PANI/PVP that comprised testing the genotoxicity and redox modulating activity was performed using human peripheral blood cells as a model system. The obtained results have shown that the investigated Ag-PANI/PVP exhibited significant prooxidant and cytostatic effects (p   lt 0.05) with no apparent potential to induce DNA damage. Although precautions should be taken to protect human health, the significant antimicrobial efficiency of Ag-PANI/PVP makes it suitable for further studies and applications in non-medical areas, such as wastewater treatment.",
journal = "New Journal of Chemistry",
title = "Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles",
pages = "12720-12711",
number = "28",
volume = "45",
doi = "10.1039/d1nj02729h"
}

Stamenovic, U., Davidović, S., Petrovic, S., Leskovac, A., Stoiljkovic, M.,& Vodnik, V.. (2021). Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles. in New Journal of Chemistry, 45(28), 12711-12720.
https://doi.org/10.1039/d1nj02729h

Stamenovic U, Davidović S, Petrovic S, Leskovac A, Stoiljkovic M, Vodnik V. Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles. in New Journal of Chemistry. 2021;45(28):12711-12720.
doi:10.1039/d1nj02729h .

Stamenovic, Una, Davidović, Slađana, Petrovic, Sandra, Leskovac, Andreja, Stoiljkovic, Milovan, Vodnik, Vesna, "Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles" in New Journal of Chemistry, 45, no. 28 (2021):12711-12720,
https://doi.org/10.1039/d1nj02729h . .

TY  - JOUR
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Gordic, Milan
AU  - Cabrera-Barjas, Gustavo
AU  - Nesic, Aleksandra
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4942
AB  - The development of edible films and coatings in the food packaging industry presents one of the modern strategies for protecting food products and ensuring their freshness and quality during their shelf lives. The application of microbial polysaccharides to the development of food package materials, as an alternative option to the commonly used plastic materials, is both economic and environmentally favorable. New edible films were developed using dextran from lactic acid bacterium Leuconostoc mesenteroides T3, and additionally plasticized by different concentrations of polyglycerol. The best tensile strength of the films was obtained using a formulation that contained 10 wt% of polyglycerol, which corresponded to a value of 4.6 MPa. The most flexible formulation, with elongation at break of 602%, was obtained with 30 wt% of polyglycerol. Water vapor permeability values of the films synthesized in this study were in the range of (3.45-8.81) * 10(-12) g/m s Pa. Such low values indicated that they could be efficient in preventing fruit from drying out during storage. Thus, the film formulations were used to coat blueberries in order to assess their quality during a storage time of 21 days at 8 degrees C. The results showed that dextran/polyglycerol films could be efficient in extending the shelf life of blueberries, which was evidenced by lower weight loss and total sugar solids values, as well as a delay in titratable acidity, in comparison to the uncoated blueberries.
T2  - Polymers
T1  - Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries
IS  - 23
VL  - 13
DO  - 10.3390/polym13234252
ER  - 

@article{
author = "Davidović, Slađana and Miljković, Miona and Gordic, Milan and Cabrera-Barjas, Gustavo and Nesic, Aleksandra and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "The development of edible films and coatings in the food packaging industry presents one of the modern strategies for protecting food products and ensuring their freshness and quality during their shelf lives. The application of microbial polysaccharides to the development of food package materials, as an alternative option to the commonly used plastic materials, is both economic and environmentally favorable. New edible films were developed using dextran from lactic acid bacterium Leuconostoc mesenteroides T3, and additionally plasticized by different concentrations of polyglycerol. The best tensile strength of the films was obtained using a formulation that contained 10 wt% of polyglycerol, which corresponded to a value of 4.6 MPa. The most flexible formulation, with elongation at break of 602%, was obtained with 30 wt% of polyglycerol. Water vapor permeability values of the films synthesized in this study were in the range of (3.45-8.81) * 10(-12) g/m s Pa. Such low values indicated that they could be efficient in preventing fruit from drying out during storage. Thus, the film formulations were used to coat blueberries in order to assess their quality during a storage time of 21 days at 8 degrees C. The results showed that dextran/polyglycerol films could be efficient in extending the shelf life of blueberries, which was evidenced by lower weight loss and total sugar solids values, as well as a delay in titratable acidity, in comparison to the uncoated blueberries.",
journal = "Polymers",
title = "Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries",
number = "23",
volume = "13",
doi = "10.3390/polym13234252"
}

Davidović, S., Miljković, M., Gordic, M., Cabrera-Barjas, G., Nesic, A.,& Dimitrijević-Branković, S.. (2021). Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries. in Polymers, 13(23).
https://doi.org/10.3390/polym13234252

Davidović S, Miljković M, Gordic M, Cabrera-Barjas G, Nesic A, Dimitrijević-Branković S. Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries. in Polymers. 2021;13(23).
doi:10.3390/polym13234252 .

Davidović, Slađana, Miljković, Miona, Gordic, Milan, Cabrera-Barjas, Gustavo, Nesic, Aleksandra, Dimitrijević-Branković, Suzana, "Dextran-Based Edible Coatings to Prolong the Shelf Life of Blueberries" in Polymers, 13, no. 23 (2021),
https://doi.org/10.3390/polym13234252 . .

TY  - JOUR
AU  - Miljković, Miona
AU  - Lazić, Vesna
AU  - Davidović, Slađana
AU  - Milivojević, Ana
AU  - Papan, Jelena
AU  - Fernandes, Margarida M.
AU  - Lanceros-Mendez, Senentxu
AU  - Ahrenkiel, Scott Phillip
AU  - Nedeljković, Jovan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4351
AB  - The aim of this study was the development of a non-toxic, biosynthetic antimicrobial agent which selectively acts on only one type of microorganism, and preserves the microbiota. Antimicrobial performance of biosynthesized silver nanoparticles (Ag NPs) by horsetail (Equisetum arvense L.) extract was examined against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus, as well as yeasts Candida albicans and Saccharomyces boulardii. Also, the cytotoxicity of Ag NPs was examined toward pre-osteoblast cells. The synthetic conditions-concentration of extract, temperature, and pH-were optimized to prepare silver colloids with different particle size distributions and long-term stability. The obtained samples were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The smaller-sized Ag NPs ( 10-20 nm), prepared at a lower temperature (20 degrees C), showed better antimicrobial performance against E. coli compared to larger ones ( 40-60 nm), prepared at high temperature (100 degrees C). On the other hand, both samples did not display any toxic action against bacteria S. aureus, or yeasts C. albicans and S. boulardii. Non-cytotoxic behavior of Ag NPs toward pre-osteoblast cells was observed for the concentrations of silver  lt = 2.25 and  lt = 4.5 mg L-1 for 10-20 and 40-60 nm-sized Ag NPs, respectively. Biosynthesized Ag NPs by horsetail extract display selective toxic action against E. coli at the ecologically acceptable concentration level.
PB  - Springer, Dordrecht
T2  - Journal of Inorganic and Organometallic Polymers and Materials
T1  - Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli
EP  - 2607
IS  - 7
SP  - 2598
VL  - 30
DO  - 10.1007/s10904-019-01402-x
ER  - 

@article{
author = "Miljković, Miona and Lazić, Vesna and Davidović, Slađana and Milivojević, Ana and Papan, Jelena and Fernandes, Margarida M. and Lanceros-Mendez, Senentxu and Ahrenkiel, Scott Phillip and Nedeljković, Jovan",
year = "2020",
abstract = "The aim of this study was the development of a non-toxic, biosynthetic antimicrobial agent which selectively acts on only one type of microorganism, and preserves the microbiota. Antimicrobial performance of biosynthesized silver nanoparticles (Ag NPs) by horsetail (Equisetum arvense L.) extract was examined against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus, as well as yeasts Candida albicans and Saccharomyces boulardii. Also, the cytotoxicity of Ag NPs was examined toward pre-osteoblast cells. The synthetic conditions-concentration of extract, temperature, and pH-were optimized to prepare silver colloids with different particle size distributions and long-term stability. The obtained samples were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The smaller-sized Ag NPs ( 10-20 nm), prepared at a lower temperature (20 degrees C), showed better antimicrobial performance against E. coli compared to larger ones ( 40-60 nm), prepared at high temperature (100 degrees C). On the other hand, both samples did not display any toxic action against bacteria S. aureus, or yeasts C. albicans and S. boulardii. Non-cytotoxic behavior of Ag NPs toward pre-osteoblast cells was observed for the concentrations of silver  lt = 2.25 and  lt = 4.5 mg L-1 for 10-20 and 40-60 nm-sized Ag NPs, respectively. Biosynthesized Ag NPs by horsetail extract display selective toxic action against E. coli at the ecologically acceptable concentration level.",
publisher = "Springer, Dordrecht",
journal = "Journal of Inorganic and Organometallic Polymers and Materials",
title = "Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli",
pages = "2607-2598",
number = "7",
volume = "30",
doi = "10.1007/s10904-019-01402-x"
}

Miljković, M., Lazić, V., Davidović, S., Milivojević, A., Papan, J., Fernandes, M. M., Lanceros-Mendez, S., Ahrenkiel, S. P.,& Nedeljković, J.. (2020). Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli. in Journal of Inorganic and Organometallic Polymers and Materials
Springer, Dordrecht., 30(7), 2598-2607.
https://doi.org/10.1007/s10904-019-01402-x

Miljković M, Lazić V, Davidović S, Milivojević A, Papan J, Fernandes MM, Lanceros-Mendez S, Ahrenkiel SP, Nedeljković J. Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli. in Journal of Inorganic and Organometallic Polymers and Materials. 2020;30(7):2598-2607.
doi:10.1007/s10904-019-01402-x .

Miljković, Miona, Lazić, Vesna, Davidović, Slađana, Milivojević, Ana, Papan, Jelena, Fernandes, Margarida M., Lanceros-Mendez, Senentxu, Ahrenkiel, Scott Phillip, Nedeljković, Jovan, "Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli" in Journal of Inorganic and Organometallic Polymers and Materials, 30, no. 7 (2020):2598-2607,
https://doi.org/10.1007/s10904-019-01402-x . .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
AU  - Dimitrijević-Branković, Suzana
AU  - Davidović, Slađana
AU  - Radovanović, Neda
AU  - Delattre, Cédric
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4479
AB  - The use of polysaccharide-based materials presents an eco-friendly technological solution, by reducing dependence on fossil resources while reducing a product's carbon footprint, when compared to conventional plastic packaging materials. This review discusses the potential of polysaccharides as a raw material to produce multifunctional materials for food packaging applications. The covered areas include the recent innovations and properties of the polysaccharide-based materials. Emphasis is given to hemicelluloses, marine polysaccharides, and bacterial exopolysaccharides and their potential application in the latest trends of food packaging materials, including edible coatings, intelligent films, and thermo-insulated aerogel packaging.
PB  - MDPI, Basel
T2  - Molecules
T1  - Prospect of Polysaccharide-Based Materials as Advanced Food Packaging
IS  - 1
VL  - 25
DO  - 10.3390/molecules25010135
ER  - 

@article{
author = "Nešić, Aleksandra and Cabrera-Barjas, Gustavo and Dimitrijević-Branković, Suzana and Davidović, Slađana and Radovanović, Neda and Delattre, Cédric",
year = "2020",
abstract = "The use of polysaccharide-based materials presents an eco-friendly technological solution, by reducing dependence on fossil resources while reducing a product's carbon footprint, when compared to conventional plastic packaging materials. This review discusses the potential of polysaccharides as a raw material to produce multifunctional materials for food packaging applications. The covered areas include the recent innovations and properties of the polysaccharide-based materials. Emphasis is given to hemicelluloses, marine polysaccharides, and bacterial exopolysaccharides and their potential application in the latest trends of food packaging materials, including edible coatings, intelligent films, and thermo-insulated aerogel packaging.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Prospect of Polysaccharide-Based Materials as Advanced Food Packaging",
number = "1",
volume = "25",
doi = "10.3390/molecules25010135"
}

Nešić, A., Cabrera-Barjas, G., Dimitrijević-Branković, S., Davidović, S., Radovanović, N.,& Delattre, C.. (2020). Prospect of Polysaccharide-Based Materials as Advanced Food Packaging. in Molecules
MDPI, Basel., 25(1).
https://doi.org/10.3390/molecules25010135

Nešić A, Cabrera-Barjas G, Dimitrijević-Branković S, Davidović S, Radovanović N, Delattre C. Prospect of Polysaccharide-Based Materials as Advanced Food Packaging. in Molecules. 2020;25(1).
doi:10.3390/molecules25010135 .

Nešić, Aleksandra, Cabrera-Barjas, Gustavo, Dimitrijević-Branković, Suzana, Davidović, Slađana, Radovanović, Neda, Delattre, Cédric, "Prospect of Polysaccharide-Based Materials as Advanced Food Packaging" in Molecules, 25, no. 1 (2020),
https://doi.org/10.3390/molecules25010135 . .

TY  - JOUR
AU  - Davidović, Slađana
AU  - Lazić, Vesna M.
AU  - Miljković, Miona
AU  - Gordić, Milan V.
AU  - Sekulić, Milica
AU  - Marinović-Cincović, Milena
AU  - Ratnayake, Ishara S.
AU  - Ahrenkiel, Scott Phillip
AU  - Nedeljković, Jovan
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4081
AB  - The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (  lt  2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10-20 nm silver nanoparticles (Ag NPs) within the agar-agar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agar-agar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inorganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureus was observed for all agar-silver films, while satisfactory results were observed for Gram-negative bacteria Pseudomonas aeruginosa (  gt = 99.6%). The release of Ag+ ions is suppressed by the increase of the concentration of Mg2+ ions and it was found to be significantly smaller (  lt = 0.24 ppm) than the harmful ecological level (1 ppm).
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions
VL  - 224
DO  - 10.1016/j.carbpol.2019.115187
ER  - 

@article{
author = "Davidović, Slađana and Lazić, Vesna M. and Miljković, Miona and Gordić, Milan V. and Sekulić, Milica and Marinović-Cincović, Milena and Ratnayake, Ishara S. and Ahrenkiel, Scott Phillip and Nedeljković, Jovan",
year = "2019",
abstract = "The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (  lt  2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10-20 nm silver nanoparticles (Ag NPs) within the agar-agar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agar-agar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inorganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureus was observed for all agar-silver films, while satisfactory results were observed for Gram-negative bacteria Pseudomonas aeruginosa (  gt = 99.6%). The release of Ag+ ions is suppressed by the increase of the concentration of Mg2+ ions and it was found to be significantly smaller (  lt = 0.24 ppm) than the harmful ecological level (1 ppm).",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions",
volume = "224",
doi = "10.1016/j.carbpol.2019.115187"
}

Davidović, S., Lazić, V. M., Miljković, M., Gordić, M. V., Sekulić, M., Marinović-Cincović, M., Ratnayake, I. S., Ahrenkiel, S. P.,& Nedeljković, J.. (2019). Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 224.
https://doi.org/10.1016/j.carbpol.2019.115187

Davidović S, Lazić VM, Miljković M, Gordić MV, Sekulić M, Marinović-Cincović M, Ratnayake IS, Ahrenkiel SP, Nedeljković J. Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions. in Carbohydrate Polymers. 2019;224.
doi:10.1016/j.carbpol.2019.115187 .

Davidović, Slađana, Lazić, Vesna M., Miljković, Miona, Gordić, Milan V., Sekulić, Milica, Marinović-Cincović, Milena, Ratnayake, Ishara S., Ahrenkiel, Scott Phillip, Nedeljković, Jovan, "Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions" in Carbohydrate Polymers, 224 (2019),
https://doi.org/10.1016/j.carbpol.2019.115187 . .

TY  - THES
AU  - Davidović, Slađana
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7279
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:21025/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51886095
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4686
AB  - The scope of this doctoral dissertation is the isolation of dextran producing lactic acid bacteria from the water kefir grain and testing the possibility of the obtained dextran for silver nanoparticles synthesis and edible film's production...
AB  - Predmet istraživanja ove doktorske disertacije je izolovanje bakterija mlečne kiseline iz zrna vodenog kefira, u cilju sinteze dekstrana, kao i ispitivanje mogućnosti primene dobijenog dekstrana za sintezu nanočestica srebra i proizvodnju jestivih filmova...
PB  - Univerzitet u Beogradu, Tehnološko-metalurški fakultet
T1  - Application of dextran from lactic acid bacteria for silver nanoparticles synthesis and edible coating production
T1  - Primena dekstrana iz bakterija mlečne kiseline za sintezu nanočestica srebra i proizvodnju jestivih filmova
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4686
ER  - 

@phdthesis{
author = "Davidović, Slađana",
year = "2019",
abstract = "The scope of this doctoral dissertation is the isolation of dextran producing lactic acid bacteria from the water kefir grain and testing the possibility of the obtained dextran for silver nanoparticles synthesis and edible film's production..., Predmet istraživanja ove doktorske disertacije je izolovanje bakterija mlečne kiseline iz zrna vodenog kefira, u cilju sinteze dekstrana, kao i ispitivanje mogućnosti primene dobijenog dekstrana za sintezu nanočestica srebra i proizvodnju jestivih filmova...",
publisher = "Univerzitet u Beogradu, Tehnološko-metalurški fakultet",
title = "Application of dextran from lactic acid bacteria for silver nanoparticles synthesis and edible coating production, Primena dekstrana iz bakterija mlečne kiseline za sintezu nanočestica srebra i proizvodnju jestivih filmova",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4686"
}

Davidović, S.. (2019). Application of dextran from lactic acid bacteria for silver nanoparticles synthesis and edible coating production. 
Univerzitet u Beogradu, Tehnološko-metalurški fakultet..
https://hdl.handle.net/21.15107/rcub_technorep_4686

Davidović S. Application of dextran from lactic acid bacteria for silver nanoparticles synthesis and edible coating production. 2019;.
https://hdl.handle.net/21.15107/rcub_technorep_4686 .

Davidović, Slađana, "Application of dextran from lactic acid bacteria for silver nanoparticles synthesis and edible coating production" (2019),
https://hdl.handle.net/21.15107/rcub_technorep_4686 .

TY  - CONF
AU  - Radovanović, Neda
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Malagurski, Ivana
AU  - Gordić, Milan
AU  - Nešić, Aleksandra
AU  - Dimitrijević-Branković, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6286
C3  - Polychar 26 World Forum on Advanced Materials
T1  - ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING
SP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6286
ER  - 

@conference{
author = "Radovanović, Neda and Miljković, Miona and Davidović, Slađana and Malagurski, Ivana and Gordić, Milan and Nešić, Aleksandra and Dimitrijević-Branković, Suzana",
year = "2018",
journal = "Polychar 26 World Forum on Advanced Materials",
title = "ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING",
pages = "94",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6286"
}

Radovanović, N., Miljković, M., Davidović, S., Malagurski, I., Gordić, M., Nešić, A.,& Dimitrijević-Branković, S.. (2018). ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING. in Polychar 26 World Forum on Advanced Materials, 94.
https://hdl.handle.net/21.15107/rcub_technorep_6286

Radovanović N, Miljković M, Davidović S, Malagurski I, Gordić M, Nešić A, Dimitrijević-Branković S. ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING. in Polychar 26 World Forum on Advanced Materials. 2018;:94.
https://hdl.handle.net/21.15107/rcub_technorep_6286 .

Radovanović, Neda, Miljković, Miona, Davidović, Slađana, Malagurski, Ivana, Gordić, Milan, Nešić, Aleksandra, Dimitrijević-Branković, Suzana, "ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING" in Polychar 26 World Forum on Advanced Materials (2018):94,
https://hdl.handle.net/21.15107/rcub_technorep_6286 .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Gordić, Milan V.
AU  - Davidović, Slađana
AU  - Radovanović, Željko
AU  - Nedeljković, Jovan
AU  - Smirnova, Irina
AU  - Gurikov, Pavel
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4006
AB  - Environmental-friendly pectin-TiO2 nanocomposite aerogels were prepared via sol-gel process and subsequent drying under supercritical conditions. The first step includes dissolution of pectin in water, addition of proper amount of TiO2 colloid and crosslinking reaction induced in the presence of tert-butanol and zinc ions. Then, the gels are subjected to the solvent exchange and supercritical CO2 drying. The influence of TiO2 nanoparticles on the textural, mechanical, thermal and antibacterial properties of aerogels was investigated. Results indicate that in the presence of TiO2 nanoparticles (NPs) mechanical, thermal and antimicrobial properties of pectin-based aerogels are improved in comparison to the control pectin aerogels. It should be emphasized that the thermal conductivity of pectin-based aerogels (0.022-0.025Wm(-1) K-1) is lower than the thermal conductivity of air. Generally, the results propose that the pectin-TiO2 nanocomposite aerogels, as bio-based material, might have potential application for the storage of temperature-sensitive food.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Pectin-based nanocomposite aerogels for potential insulated food packaging application
EP  - 135
SP  - 128
VL  - 195
DO  - 10.1016/j.carbpol.2018.04.076
ER  - 

@article{
author = "Nešić, Aleksandra and Gordić, Milan V. and Davidović, Slađana and Radovanović, Željko and Nedeljković, Jovan and Smirnova, Irina and Gurikov, Pavel",
year = "2018",
abstract = "Environmental-friendly pectin-TiO2 nanocomposite aerogels were prepared via sol-gel process and subsequent drying under supercritical conditions. The first step includes dissolution of pectin in water, addition of proper amount of TiO2 colloid and crosslinking reaction induced in the presence of tert-butanol and zinc ions. Then, the gels are subjected to the solvent exchange and supercritical CO2 drying. The influence of TiO2 nanoparticles on the textural, mechanical, thermal and antibacterial properties of aerogels was investigated. Results indicate that in the presence of TiO2 nanoparticles (NPs) mechanical, thermal and antimicrobial properties of pectin-based aerogels are improved in comparison to the control pectin aerogels. It should be emphasized that the thermal conductivity of pectin-based aerogels (0.022-0.025Wm(-1) K-1) is lower than the thermal conductivity of air. Generally, the results propose that the pectin-TiO2 nanocomposite aerogels, as bio-based material, might have potential application for the storage of temperature-sensitive food.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Pectin-based nanocomposite aerogels for potential insulated food packaging application",
pages = "135-128",
volume = "195",
doi = "10.1016/j.carbpol.2018.04.076"
}

Nešić, A., Gordić, M. V., Davidović, S., Radovanović, Ž., Nedeljković, J., Smirnova, I.,& Gurikov, P.. (2018). Pectin-based nanocomposite aerogels for potential insulated food packaging application. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 195, 128-135.
https://doi.org/10.1016/j.carbpol.2018.04.076

Nešić A, Gordić MV, Davidović S, Radovanović Ž, Nedeljković J, Smirnova I, Gurikov P. Pectin-based nanocomposite aerogels for potential insulated food packaging application. in Carbohydrate Polymers. 2018;195:128-135.
doi:10.1016/j.carbpol.2018.04.076 .

Nešić, Aleksandra, Gordić, Milan V., Davidović, Slađana, Radovanović, Željko, Nedeljković, Jovan, Smirnova, Irina, Gurikov, Pavel, "Pectin-based nanocomposite aerogels for potential insulated food packaging application" in Carbohydrate Polymers, 195 (2018):128-135,
https://doi.org/10.1016/j.carbpol.2018.04.076 . .

TY  - JOUR
AU  - Miljković, Miona
AU  - Lazić, Vesna M.
AU  - Banjanac, Katarina
AU  - Davidović, Slađana
AU  - Bezbradica, Dejan
AU  - Marinković, Aleksandar
AU  - Sredojević, Dušan
AU  - Nedeljković, Jovan
AU  - Dimitrijević-Branković, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4014
AB  - The TiO2 based hybrid supports with different functional groups (amino, glutaraldehyde or epoxy) were prepared and their influence on immobilization of dextransucrase (DS) was studied. Novel synthetic route for surface modification of TiO2 with amino and glutaraldehyde groups was developed taking advantage of charge transfer complex (CTC) formation between surface Ti atoms and salicylate-type of ligand (5 aminosalicylic acid (5-ASA)). The proposed coordination of 5-ASA to the surface of TiO2 powder and optical properties of CTC was presented. The pristine TiO2 and amino functionalized TiO2 have higher sorption capacity for DS (12.6 and 12.0 mg g(-1), respectively) compared to glutaraldehyde and epoxy activated supports (9.6 and 9.8 mg g(-1) respectively). However, immobilized enzyme to either glutaraldehyde or epoxy functionalized TiO2 have almost two times higher expressed activities compared to pristine TiO2 support (258, 235 and 142 IU g(-1), respectively). Thermal stability of enzyme immobilized on glutaraldehyde and epoxy functionalized supports was studied at 40 degrees C, as well as operational stability under long-run working conditions in repeated cycles. After five cycles, DS imobilized on glutaraldehyde activated support retained almost 70% of its initial expressed activity, while, after five cycles, performance of DS immobilized on epoxy activated support was significantly lower (15%).
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Immobilization of dextransucrase on functionalized TiO2 supports
EP  - 1223
SP  - 1216
VL  - 114
DO  - 10.1016/j.ijbiomac.2018.04.027
ER  - 

@article{
author = "Miljković, Miona and Lazić, Vesna M. and Banjanac, Katarina and Davidović, Slađana and Bezbradica, Dejan and Marinković, Aleksandar and Sredojević, Dušan and Nedeljković, Jovan and Dimitrijević-Branković, Suzana",
year = "2018",
abstract = "The TiO2 based hybrid supports with different functional groups (amino, glutaraldehyde or epoxy) were prepared and their influence on immobilization of dextransucrase (DS) was studied. Novel synthetic route for surface modification of TiO2 with amino and glutaraldehyde groups was developed taking advantage of charge transfer complex (CTC) formation between surface Ti atoms and salicylate-type of ligand (5 aminosalicylic acid (5-ASA)). The proposed coordination of 5-ASA to the surface of TiO2 powder and optical properties of CTC was presented. The pristine TiO2 and amino functionalized TiO2 have higher sorption capacity for DS (12.6 and 12.0 mg g(-1), respectively) compared to glutaraldehyde and epoxy activated supports (9.6 and 9.8 mg g(-1) respectively). However, immobilized enzyme to either glutaraldehyde or epoxy functionalized TiO2 have almost two times higher expressed activities compared to pristine TiO2 support (258, 235 and 142 IU g(-1), respectively). Thermal stability of enzyme immobilized on glutaraldehyde and epoxy functionalized supports was studied at 40 degrees C, as well as operational stability under long-run working conditions in repeated cycles. After five cycles, DS imobilized on glutaraldehyde activated support retained almost 70% of its initial expressed activity, while, after five cycles, performance of DS immobilized on epoxy activated support was significantly lower (15%).",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Immobilization of dextransucrase on functionalized TiO2 supports",
pages = "1223-1216",
volume = "114",
doi = "10.1016/j.ijbiomac.2018.04.027"
}

Miljković, M., Lazić, V. M., Banjanac, K., Davidović, S., Bezbradica, D., Marinković, A., Sredojević, D., Nedeljković, J.,& Dimitrijević-Branković, S.. (2018). Immobilization of dextransucrase on functionalized TiO2 supports. in International Journal of Biological Macromolecules
Elsevier Science Bv, Amsterdam., 114, 1216-1223.
https://doi.org/10.1016/j.ijbiomac.2018.04.027

Miljković M, Lazić VM, Banjanac K, Davidović S, Bezbradica D, Marinković A, Sredojević D, Nedeljković J, Dimitrijević-Branković S. Immobilization of dextransucrase on functionalized TiO2 supports. in International Journal of Biological Macromolecules. 2018;114:1216-1223.
doi:10.1016/j.ijbiomac.2018.04.027 .

Miljković, Miona, Lazić, Vesna M., Banjanac, Katarina, Davidović, Slađana, Bezbradica, Dejan, Marinković, Aleksandar, Sredojević, Dušan, Nedeljković, Jovan, Dimitrijević-Branković, Suzana, "Immobilization of dextransucrase on functionalized TiO2 supports" in International Journal of Biological Macromolecules, 114 (2018):1216-1223,
https://doi.org/10.1016/j.ijbiomac.2018.04.027 . .

TY  - JOUR
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Tomić, Miloš
AU  - Gordić, Milan V.
AU  - Nešić, Aleksandra
AU  - Dimitrijević, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4017
AB  - The aim of this study was to develop dextran-based edible films plasticized by sorbitol. In order to optimise the film-forming formulation, response surface methodology was used. The influence of dextran and sorbitol concentration on the mechanical and water vapour barrier properties of obtained films was investigated. The results showed that both parameters exhibited significant effect on the water vapour permeability of a film. Both dextran and sorbitol concentration had significant influence on tensile strength and elongation at break, whereas only sorbitol concentration had significant effect on Young's modulus. After optimisation by desirability approach, it was found that a film made of 3.40 wt% of dextran and 20.43 wt% of sorbitol showed the lowest water vapour permeability and the highest tensile strength and elasticity.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3
EP  - 213
SP  - 207
VL  - 184
DO  - 10.1016/j.carbpol.2017.12.061
ER  - 

@article{
author = "Davidović, Slađana and Miljković, Miona and Tomić, Miloš and Gordić, Milan V. and Nešić, Aleksandra and Dimitrijević, Suzana",
year = "2018",
abstract = "The aim of this study was to develop dextran-based edible films plasticized by sorbitol. In order to optimise the film-forming formulation, response surface methodology was used. The influence of dextran and sorbitol concentration on the mechanical and water vapour barrier properties of obtained films was investigated. The results showed that both parameters exhibited significant effect on the water vapour permeability of a film. Both dextran and sorbitol concentration had significant influence on tensile strength and elongation at break, whereas only sorbitol concentration had significant effect on Young's modulus. After optimisation by desirability approach, it was found that a film made of 3.40 wt% of dextran and 20.43 wt% of sorbitol showed the lowest water vapour permeability and the highest tensile strength and elasticity.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3",
pages = "213-207",
volume = "184",
doi = "10.1016/j.carbpol.2017.12.061"
}

Davidović, S., Miljković, M., Tomić, M., Gordić, M. V., Nešić, A.,& Dimitrijević, S.. (2018). Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 184, 207-213.
https://doi.org/10.1016/j.carbpol.2017.12.061

Davidović S, Miljković M, Tomić M, Gordić MV, Nešić A, Dimitrijević S. Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3. in Carbohydrate Polymers. 2018;184:207-213.
doi:10.1016/j.carbpol.2017.12.061 .

Davidović, Slađana, Miljković, Miona, Tomić, Miloš, Gordić, Milan V., Nešić, Aleksandra, Dimitrijević, Suzana, "Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3" in Carbohydrate Polymers, 184 (2018):207-213,
https://doi.org/10.1016/j.carbpol.2017.12.061 . .