TY  - JOUR
AU  - Nowak, Mariusz
AU  - Semba, Damian
AU  - Mišić, Dušan
AU  - Półbrat, Tomasz
AU  - Stojanović, Dušica
AU  - Stanojević, Slavoljub
AU  - Trusek, Anna
AU  - Žižović, Irena
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6614
AB  - The study reports an effluent-free, green process for the production of materials with favorable antibiofilm properties by cellulose acetate grafting with quaternary ammonium compounds in supercritical carbon dioxide. Two quaternary ammonium compounds, N-(2-Hydroxyethyl)-N,N-dimethylundecan-1-aminium Bromide and N-(11-Hydroxyundecanyl-N,N-dimethyltetradecan- 1-aminium Bromide, were synthesized and chemically attached to cellulose acetate via hexamethylene diisocyanate as a linker under the conditions of 30 MPa and 70 °C. The polymer modification occurred in its whole volume. The chemical conversion led to a decrease in the degree of crystallinity and the appearance of a rugged polymer surface. However, the cross-section imaging of the newly obtained materials revealed a compact polymer structure. The functionalized materials acquired hydrophobic properties. Microbiological tests showed the impossibility of Staphylococcus aureus ATCC 29213, Methicillin-resistant Staphylococcus aureus ATCC 43300, Escherichia coli ATCC 10536, and Salmonella Enteritidis ATCC 13076 attachment to the material obtained by the cellulose acetate grafting procedure with N-(11-Hydroxyundecanyl-N,N-dimethyltetradecan- 1-aminium Bromide.
PB  - Elsevier B.V.
T2  - Journal of Supercritical Fluids
T1  - The transformation of cellulose acetate into a new biocidal polymer by effluent-free grafting in supercritical carbon dioxide
SP  - 106058
VL  - 202
DO  - 10.1016/j.supflu.2023.106058
ER  - 

@article{
author = "Nowak, Mariusz and Semba, Damian and Mišić, Dušan and Półbrat, Tomasz and Stojanović, Dušica and Stanojević, Slavoljub and Trusek, Anna and Žižović, Irena",
year = "2023",
abstract = "The study reports an effluent-free, green process for the production of materials with favorable antibiofilm properties by cellulose acetate grafting with quaternary ammonium compounds in supercritical carbon dioxide. Two quaternary ammonium compounds, N-(2-Hydroxyethyl)-N,N-dimethylundecan-1-aminium Bromide and N-(11-Hydroxyundecanyl-N,N-dimethyltetradecan- 1-aminium Bromide, were synthesized and chemically attached to cellulose acetate via hexamethylene diisocyanate as a linker under the conditions of 30 MPa and 70 °C. The polymer modification occurred in its whole volume. The chemical conversion led to a decrease in the degree of crystallinity and the appearance of a rugged polymer surface. However, the cross-section imaging of the newly obtained materials revealed a compact polymer structure. The functionalized materials acquired hydrophobic properties. Microbiological tests showed the impossibility of Staphylococcus aureus ATCC 29213, Methicillin-resistant Staphylococcus aureus ATCC 43300, Escherichia coli ATCC 10536, and Salmonella Enteritidis ATCC 13076 attachment to the material obtained by the cellulose acetate grafting procedure with N-(11-Hydroxyundecanyl-N,N-dimethyltetradecan- 1-aminium Bromide.",
publisher = "Elsevier B.V.",
journal = "Journal of Supercritical Fluids",
title = "The transformation of cellulose acetate into a new biocidal polymer by effluent-free grafting in supercritical carbon dioxide",
pages = "106058",
volume = "202",
doi = "10.1016/j.supflu.2023.106058"
}

Nowak, M., Semba, D., Mišić, D., Półbrat, T., Stojanović, D., Stanojević, S., Trusek, A.,& Žižović, I.. (2023). The transformation of cellulose acetate into a new biocidal polymer by effluent-free grafting in supercritical carbon dioxide. in Journal of Supercritical Fluids
Elsevier B.V.., 202, 106058.
https://doi.org/10.1016/j.supflu.2023.106058

Nowak M, Semba D, Mišić D, Półbrat T, Stojanović D, Stanojević S, Trusek A, Žižović I. The transformation of cellulose acetate into a new biocidal polymer by effluent-free grafting in supercritical carbon dioxide. in Journal of Supercritical Fluids. 2023;202:106058.
doi:10.1016/j.supflu.2023.106058 .

Nowak, Mariusz, Semba, Damian, Mišić, Dušan, Półbrat, Tomasz, Stojanović, Dušica, Stanojević, Slavoljub, Trusek, Anna, Žižović, Irena, "The transformation of cellulose acetate into a new biocidal polymer by effluent-free grafting in supercritical carbon dioxide" in Journal of Supercritical Fluids, 202 (2023):106058,
https://doi.org/10.1016/j.supflu.2023.106058 . .

TY  - CONF
AU  - Nowak, Mariusz
AU  - Semba, Damian
AU  - Mišić, Dušan
AU  - Półbrat, Tomasz
AU  - Stojanović, Dušica
AU  - Trusek, Anna
AU  - Žižović, Irena
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6937
AB  - There is an urgency to develop novel materials capable of preventing the adherence of microorganisms to
their surface. Such materials with antibiofilm properties are needed in hospitals and public places, animal
farms and veterinary hospitals, and biofouling prevention. Biofouling is the phenomenon of adhesion and
the growth of microorganisms on surfaces in contact with water. It poses a significant problem for filtration
processes, water treatment units, bioreactors, and ships performance (marine biofouling). High-pressure
techniques such as supercritical solvent impregnation and grafting in the supercritical phase allow for the
modification of a solid matrix throughout the whole volume and production of materials of unique
properties in an environmentally friendly way. Grafting involves the chemical attachment of an active
compound to a solid matrix, ensuring its long-lasting properties. Grafting in supercritical carbon dioxide
(scCO2) has been reported as an efficient tool in the production of materials with antibacterial1
, and recently
also antibiofilm properties2
. This study aims to investigate the potential of cellulose acetate (CA) grafting
with quaternary ammonium compounds (QAC) for acquiring antibiofilm properties. Two QAC were
synthesized, and the grafting conditions were investigated. The grafting was performed via hexamethylene
diisocyanate (HDI) as a linker1
. Obtained materials were analyzed using FTIR, SEM and Ion microscopy,
DSC analyses, contact angle measurements, and standardized microbiological procedures to evaluate
antibiofilm properties.
C3  - 13th International Symposium on Supercritical Fluids Montreal, ISSF2022, Canada, May 15-18, 2022
T1  - Cellulose Acetate Modification Towards Antibiofilm Properties via Chemical Attachment of Quaternary Ammonium Compounds using Supercritical CO2
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6937
ER  - 

@conference{
author = "Nowak, Mariusz and Semba, Damian and Mišić, Dušan and Półbrat, Tomasz and Stojanović, Dušica and Trusek, Anna and Žižović, Irena",
year = "2022",
abstract = "There is an urgency to develop novel materials capable of preventing the adherence of microorganisms to
their surface. Such materials with antibiofilm properties are needed in hospitals and public places, animal
farms and veterinary hospitals, and biofouling prevention. Biofouling is the phenomenon of adhesion and
the growth of microorganisms on surfaces in contact with water. It poses a significant problem for filtration
processes, water treatment units, bioreactors, and ships performance (marine biofouling). High-pressure
techniques such as supercritical solvent impregnation and grafting in the supercritical phase allow for the
modification of a solid matrix throughout the whole volume and production of materials of unique
properties in an environmentally friendly way. Grafting involves the chemical attachment of an active
compound to a solid matrix, ensuring its long-lasting properties. Grafting in supercritical carbon dioxide
(scCO2) has been reported as an efficient tool in the production of materials with antibacterial1
, and recently
also antibiofilm properties2
. This study aims to investigate the potential of cellulose acetate (CA) grafting
with quaternary ammonium compounds (QAC) for acquiring antibiofilm properties. Two QAC were
synthesized, and the grafting conditions were investigated. The grafting was performed via hexamethylene
diisocyanate (HDI) as a linker1
. Obtained materials were analyzed using FTIR, SEM and Ion microscopy,
DSC analyses, contact angle measurements, and standardized microbiological procedures to evaluate
antibiofilm properties.",
journal = "13th International Symposium on Supercritical Fluids Montreal, ISSF2022, Canada, May 15-18, 2022",
title = "Cellulose Acetate Modification Towards Antibiofilm Properties via Chemical Attachment of Quaternary Ammonium Compounds using Supercritical CO2",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6937"
}

Nowak, M., Semba, D., Mišić, D., Półbrat, T., Stojanović, D., Trusek, A.,& Žižović, I.. (2022). Cellulose Acetate Modification Towards Antibiofilm Properties via Chemical Attachment of Quaternary Ammonium Compounds using Supercritical CO2. in 13th International Symposium on Supercritical Fluids Montreal, ISSF2022, Canada, May 15-18, 2022.
https://hdl.handle.net/21.15107/rcub_technorep_6937

Nowak M, Semba D, Mišić D, Półbrat T, Stojanović D, Trusek A, Žižović I. Cellulose Acetate Modification Towards Antibiofilm Properties via Chemical Attachment of Quaternary Ammonium Compounds using Supercritical CO2. in 13th International Symposium on Supercritical Fluids Montreal, ISSF2022, Canada, May 15-18, 2022. 2022;.
https://hdl.handle.net/21.15107/rcub_technorep_6937 .

Nowak, Mariusz, Semba, Damian, Mišić, Dušan, Półbrat, Tomasz, Stojanović, Dušica, Trusek, Anna, Žižović, Irena, "Cellulose Acetate Modification Towards Antibiofilm Properties via Chemical Attachment of Quaternary Ammonium Compounds using Supercritical CO2" in 13th International Symposium on Supercritical Fluids Montreal, ISSF2022, Canada, May 15-18, 2022 (2022),
https://hdl.handle.net/21.15107/rcub_technorep_6937 .

TY  - BOOK
AU  - Tadić, Vanja
AU  - Maksimović, Svetolik
AU  - Nešić, Ivana
AU  - Žižović, Irena
AU  - Mišić, Dušan
AU  - Milovanović, Stoja
AU  - Žugić, Ana
AU  - Aksentijević, Ksenija
AU  - Stanković, Milica
AU  - Ivanović, Jasna
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6876
PB  - Niš : Galaksijanis
T1  - Smilje od tradicionalne do naučno zasnovane primene
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6876
ER  - 

@book{
author = "Tadić, Vanja and Maksimović, Svetolik and Nešić, Ivana and Žižović, Irena and Mišić, Dušan and Milovanović, Stoja and Žugić, Ana and Aksentijević, Ksenija and Stanković, Milica and Ivanović, Jasna",
year = "2021",
publisher = "Niš : Galaksijanis",
title = "Smilje od tradicionalne do naučno zasnovane primene",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6876"
}

Tadić, V., Maksimović, S., Nešić, I., Žižović, I., Mišić, D., Milovanović, S., Žugić, A., Aksentijević, K., Stanković, M.,& Ivanović, J.. (2021). Smilje od tradicionalne do naučno zasnovane primene. 
Niš : Galaksijanis..
https://hdl.handle.net/21.15107/rcub_technorep_6876

Tadić V, Maksimović S, Nešić I, Žižović I, Mišić D, Milovanović S, Žugić A, Aksentijević K, Stanković M, Ivanović J. Smilje od tradicionalne do naučno zasnovane primene. 2021;.
https://hdl.handle.net/21.15107/rcub_technorep_6876 .

Tadić, Vanja, Maksimović, Svetolik, Nešić, Ivana, Žižović, Irena, Mišić, Dušan, Milovanović, Stoja, Žugić, Ana, Aksentijević, Ksenija, Stanković, Milica, Ivanović, Jasna, "Smilje od tradicionalne do naučno zasnovane primene" (2021),
https://hdl.handle.net/21.15107/rcub_technorep_6876 .

TY  - JOUR
AU  - Pajnik, Jelena
AU  - Lukić, Ivana
AU  - Dikić, Jelena
AU  - Ašanin, Jelena
AU  - Gordić, Milan V.
AU  - Mišić, Dušan
AU  - Žižović, Irena
AU  - Korzeniowska, Malgorzata
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4442
AB  - In the present study, supercritical solvent impregnation (SSI) has been applied to incorporate thymol into bio-composite polymers as a potential active packaging material. Thymol, a natural component with a proven antimicrobial activity, was successfully impregnated into starch-chitosan (SC) and starch-chitosan-zeolite (SCZ) films using supercritical carbon dioxide (scCO(2)) as a solvent. Experiments were performed at 35 degrees C, pressures of 15.5 and 30 MPa, and an impregnation time in the range of 4-24 h. The highest impregnation yields of SC films with starch to chitosan mass ratios of 1:1 and 1:2 were 10.80% and 6.48%, respectively. The addition of natural zeolite (15-60%) significantly increased the loading capacity of films enabling thymol incorporation in a quantity of 16.7-27.3%. FTIR and SEM analyses were applied for the characterization of the films. Mechanical properties and water vapor permeability of films before and after the impregnation were tested as well. Thymol release kinetics in deionized water was followed and modeled by the Korsmeyer-Peppas and Weibull model. SCZ films with thymol loading of approximately 24% exhibited strong antibacterial activity against E. coli and methicillin-resistant Staphylococcus (S.) aureus (MRSA).
PB  - MDPI, Basel
T2  - Molecules
T1  - Application of Supercritical Solvent Impregnation for Production of Zeolite Modified Starch-Chitosan Polymers with Antibacterial Properties
IS  - 20
VL  - 25
DO  - 10.3390/molecules25204717
ER  - 

@article{
author = "Pajnik, Jelena and Lukić, Ivana and Dikić, Jelena and Ašanin, Jelena and Gordić, Milan V. and Mišić, Dušan and Žižović, Irena and Korzeniowska, Malgorzata",
year = "2020",
abstract = "In the present study, supercritical solvent impregnation (SSI) has been applied to incorporate thymol into bio-composite polymers as a potential active packaging material. Thymol, a natural component with a proven antimicrobial activity, was successfully impregnated into starch-chitosan (SC) and starch-chitosan-zeolite (SCZ) films using supercritical carbon dioxide (scCO(2)) as a solvent. Experiments were performed at 35 degrees C, pressures of 15.5 and 30 MPa, and an impregnation time in the range of 4-24 h. The highest impregnation yields of SC films with starch to chitosan mass ratios of 1:1 and 1:2 were 10.80% and 6.48%, respectively. The addition of natural zeolite (15-60%) significantly increased the loading capacity of films enabling thymol incorporation in a quantity of 16.7-27.3%. FTIR and SEM analyses were applied for the characterization of the films. Mechanical properties and water vapor permeability of films before and after the impregnation were tested as well. Thymol release kinetics in deionized water was followed and modeled by the Korsmeyer-Peppas and Weibull model. SCZ films with thymol loading of approximately 24% exhibited strong antibacterial activity against E. coli and methicillin-resistant Staphylococcus (S.) aureus (MRSA).",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Application of Supercritical Solvent Impregnation for Production of Zeolite Modified Starch-Chitosan Polymers with Antibacterial Properties",
number = "20",
volume = "25",
doi = "10.3390/molecules25204717"
}

Pajnik, J., Lukić, I., Dikić, J., Ašanin, J., Gordić, M. V., Mišić, D., Žižović, I.,& Korzeniowska, M.. (2020). Application of Supercritical Solvent Impregnation for Production of Zeolite Modified Starch-Chitosan Polymers with Antibacterial Properties. in Molecules
MDPI, Basel., 25(20).
https://doi.org/10.3390/molecules25204717

Pajnik J, Lukić I, Dikić J, Ašanin J, Gordić MV, Mišić D, Žižović I, Korzeniowska M. Application of Supercritical Solvent Impregnation for Production of Zeolite Modified Starch-Chitosan Polymers with Antibacterial Properties. in Molecules. 2020;25(20).
doi:10.3390/molecules25204717 .

Pajnik, Jelena, Lukić, Ivana, Dikić, Jelena, Ašanin, Jelena, Gordić, Milan V., Mišić, Dušan, Žižović, Irena, Korzeniowska, Malgorzata, "Application of Supercritical Solvent Impregnation for Production of Zeolite Modified Starch-Chitosan Polymers with Antibacterial Properties" in Molecules, 25, no. 20 (2020),
https://doi.org/10.3390/molecules25204717 . .

TY  - JOUR
AU  - Marković, Darka
AU  - Vasiljević, Jelena
AU  - Ašanin, Jelena
AU  - Ilić‐Tomić, Tatjana
AU  - Tomšič, Brigita
AU  - Jokić, Bojan
AU  - Mitrić, Miodrag
AU  - Simončič, Barbara
AU  - Mišić, Dušan
AU  - Radetić, Maja
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4520
AB  - A novel impregnation process for the fabrication of cotton nanocomposite with strong antimicrobial activity against antibiotics-resistant bacteria and yeast was developed. The impregnation process includes the sol-gel treatment of fabric with (3-aminopropyl)triethoxysilane in the first step, and synthesis of the CuO/Cu2O nanoparticles (NPs) on the fabric surface in the second step. The in situ synthesis of the CuO/Cu2O NPs was based on the adsorption of Cu2+-ions by the introduced amino groups of the sol-gel coating. The adsorbed Cu2+-ions are subsequently reduced in the alkaline solution of NaBH4. X-ray diffraction measurements confirmed the formation of CuO/Cu2O NPs. Scanning electron microscopy and atomic absorption spectrometry analyses indicate that the particle size, agglomeration, and amounts of synthesized NPs were highly affected by the initial concentration of CuSO(4)solution. The toxicity of nanocomposites to human keratinocytes (HaCaT) and antimicrobial activity against Gram-negativeEscherichia coliATCC 25922,E. coliATCC BAA 2469, andKlebsiella pneumoniaeATCC BAA 2146, and Gram-positive bacteriaStaphylococcus aureusATCC 25923,S. aureusATCC 43300 and yeastCandida albicansATCC 24433 strongly depended on the copper content. In addition to excellent antimicrobial activity, controlled release of Cu2+-ions from the fabrics into physiological saline solution was obtained.
PB  - Wiley, Hoboken
T2  - Journal of Applied Polymer Science
T1  - The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions
IS  - 40
VL  - 137
DO  - 10.1002/app.49194
ER  - 

@article{
author = "Marković, Darka and Vasiljević, Jelena and Ašanin, Jelena and Ilić‐Tomić, Tatjana and Tomšič, Brigita and Jokić, Bojan and Mitrić, Miodrag and Simončič, Barbara and Mišić, Dušan and Radetić, Maja",
year = "2020",
abstract = "A novel impregnation process for the fabrication of cotton nanocomposite with strong antimicrobial activity against antibiotics-resistant bacteria and yeast was developed. The impregnation process includes the sol-gel treatment of fabric with (3-aminopropyl)triethoxysilane in the first step, and synthesis of the CuO/Cu2O nanoparticles (NPs) on the fabric surface in the second step. The in situ synthesis of the CuO/Cu2O NPs was based on the adsorption of Cu2+-ions by the introduced amino groups of the sol-gel coating. The adsorbed Cu2+-ions are subsequently reduced in the alkaline solution of NaBH4. X-ray diffraction measurements confirmed the formation of CuO/Cu2O NPs. Scanning electron microscopy and atomic absorption spectrometry analyses indicate that the particle size, agglomeration, and amounts of synthesized NPs were highly affected by the initial concentration of CuSO(4)solution. The toxicity of nanocomposites to human keratinocytes (HaCaT) and antimicrobial activity against Gram-negativeEscherichia coliATCC 25922,E. coliATCC BAA 2469, andKlebsiella pneumoniaeATCC BAA 2146, and Gram-positive bacteriaStaphylococcus aureusATCC 25923,S. aureusATCC 43300 and yeastCandida albicansATCC 24433 strongly depended on the copper content. In addition to excellent antimicrobial activity, controlled release of Cu2+-ions from the fabrics into physiological saline solution was obtained.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Polymer Science",
title = "The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions",
number = "40",
volume = "137",
doi = "10.1002/app.49194"
}

Marković, D., Vasiljević, J., Ašanin, J., Ilić‐Tomić, T., Tomšič, B., Jokić, B., Mitrić, M., Simončič, B., Mišić, D.,& Radetić, M.. (2020). The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions. in Journal of Applied Polymer Science
Wiley, Hoboken., 137(40).
https://doi.org/10.1002/app.49194

Marković D, Vasiljević J, Ašanin J, Ilić‐Tomić T, Tomšič B, Jokić B, Mitrić M, Simončič B, Mišić D, Radetić M. The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions. in Journal of Applied Polymer Science. 2020;137(40).
doi:10.1002/app.49194 .

Marković, Darka, Vasiljević, Jelena, Ašanin, Jelena, Ilić‐Tomić, Tatjana, Tomšič, Brigita, Jokić, Bojan, Mitrić, Miodrag, Simončič, Barbara, Mišić, Dušan, Radetić, Maja, "The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions" in Journal of Applied Polymer Science, 137, no. 40 (2020),
https://doi.org/10.1002/app.49194 . .

TY  - JOUR
AU  - Marković, Darka
AU  - Ašanin, Jelena
AU  - Nunney, Tim
AU  - Radovanović, Željko
AU  - Radoičić, Marija B.
AU  - Mitrić, Miodrag
AU  - Mišić, Dušan
AU  - Radetić, Maja
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4106
AB  - This study discusses the possibility of fabrication of textile nanocomposites with antimicrobial activity against antibiotics-resistant bacterial strains and yeast. Modification of cotton fabric with oxalic acid solutions of different concentrations provided free carboxyl groups for binding of Cu2+ -ions from copper (II) sulfate solution which were further reduced with sodium borohydride in alkaline solution. An increase in the concentration of applied oxalic acid resulted in larger amounts of free carboxyl groups on the cotton fibers, Cu2+ -ions uptake and total amounts of Cu-based nanoparticles after reduction. XPS and XRD analyses suggested that nanoparticles mainly consisted of CuO with fractions of Cu2O. Fabricated textile nanocomposites ensured maximum reduction of Gram-negative E. coli ATCC 25922, E. coli NCTC 13846, E. coli ATCC BAA-2469, K. pneumoniae ATCC-BAA 2146 and P. aeruginosa ATCC 27853, Gram-positive bacteria S. aureus ATCC 25923 and S. aureus ATCC 43300 and yeast C. albicans ATCC 24433. Additionally, controlled release of Cu2+ -ions from fabrics into the physiological saline solution was obtained within 24 hours.
PB  - Korean Fiber Soc, Seoul
T2  - Fibers and Polymers
T1  - Broad Spectrum of Antimicrobial Activity of Cotton Fabric Modified with Oxalic Acid and CuO/Cu2O Nanoparticles
EP  - 2325
IS  - 11
SP  - 2317
VL  - 20
DO  - 10.1007/s12221-019-9131-5
ER  - 

@article{
author = "Marković, Darka and Ašanin, Jelena and Nunney, Tim and Radovanović, Željko and Radoičić, Marija B. and Mitrić, Miodrag and Mišić, Dušan and Radetić, Maja",
year = "2019",
abstract = "This study discusses the possibility of fabrication of textile nanocomposites with antimicrobial activity against antibiotics-resistant bacterial strains and yeast. Modification of cotton fabric with oxalic acid solutions of different concentrations provided free carboxyl groups for binding of Cu2+ -ions from copper (II) sulfate solution which were further reduced with sodium borohydride in alkaline solution. An increase in the concentration of applied oxalic acid resulted in larger amounts of free carboxyl groups on the cotton fibers, Cu2+ -ions uptake and total amounts of Cu-based nanoparticles after reduction. XPS and XRD analyses suggested that nanoparticles mainly consisted of CuO with fractions of Cu2O. Fabricated textile nanocomposites ensured maximum reduction of Gram-negative E. coli ATCC 25922, E. coli NCTC 13846, E. coli ATCC BAA-2469, K. pneumoniae ATCC-BAA 2146 and P. aeruginosa ATCC 27853, Gram-positive bacteria S. aureus ATCC 25923 and S. aureus ATCC 43300 and yeast C. albicans ATCC 24433. Additionally, controlled release of Cu2+ -ions from fabrics into the physiological saline solution was obtained within 24 hours.",
publisher = "Korean Fiber Soc, Seoul",
journal = "Fibers and Polymers",
title = "Broad Spectrum of Antimicrobial Activity of Cotton Fabric Modified with Oxalic Acid and CuO/Cu2O Nanoparticles",
pages = "2325-2317",
number = "11",
volume = "20",
doi = "10.1007/s12221-019-9131-5"
}

Marković, D., Ašanin, J., Nunney, T., Radovanović, Ž., Radoičić, M. B., Mitrić, M., Mišić, D.,& Radetić, M.. (2019). Broad Spectrum of Antimicrobial Activity of Cotton Fabric Modified with Oxalic Acid and CuO/Cu2O Nanoparticles. in Fibers and Polymers
Korean Fiber Soc, Seoul., 20(11), 2317-2325.
https://doi.org/10.1007/s12221-019-9131-5

Marković D, Ašanin J, Nunney T, Radovanović Ž, Radoičić MB, Mitrić M, Mišić D, Radetić M. Broad Spectrum of Antimicrobial Activity of Cotton Fabric Modified with Oxalic Acid and CuO/Cu2O Nanoparticles. in Fibers and Polymers. 2019;20(11):2317-2325.
doi:10.1007/s12221-019-9131-5 .

Marković, Darka, Ašanin, Jelena, Nunney, Tim, Radovanović, Željko, Radoičić, Marija B., Mitrić, Miodrag, Mišić, Dušan, Radetić, Maja, "Broad Spectrum of Antimicrobial Activity of Cotton Fabric Modified with Oxalic Acid and CuO/Cu2O Nanoparticles" in Fibers and Polymers, 20, no. 11 (2019):2317-2325,
https://doi.org/10.1007/s12221-019-9131-5 . .

TY  - JOUR
AU  - Marković, Darka
AU  - Jokić, Bojan
AU  - Radovanović, Željko
AU  - Ašanin, Jelena
AU  - Radoičić, Marija B.
AU  - Mitrić, Miodrag
AU  - Mišić, Dušan
AU  - Radetić, Maja
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4193
AB  - This study discusses the possibility of in situ synthesis of Cu-based nanoparticles (NPs) on cotton and viscose rayon fabrics previously modified with 1,2,3,4-butanetetracarboxylic acid (BTCA) of different concentrations, with the aim to obtain antibacterial protection. The changes in the chemical structure of the fibers caused by the modification with BTCA were assessed by FTIR spectroscopy. The higher the concentration of the applied BTCA, the larger the amounts of free carboxyl groups in both fabrics. AAS analysis showed that the samples modified with higher concentration of BTCA provided larger Cu2+-ions uptake and consequently, higher content of Cu after Cu2+-ions reduction. The presence of Cu-based NPs on the fabrics was confirmed by FESEM and EDS analyses. XRD analysis revealed that NPs appeared as a mixture of CuO and Cu2O. Synthesized NPs imparted excellent antibacterial activity to both fabrics against E. coli and S. aureus, including methicillin-resistant S. aureus.
PB  - Editura Acad Romane, Bucuresti
T2  - Cellulose Chemistry and Technology
T1  - Influence of 1,2,3,4-butanetetracarboxylic acid concentration on in situ synthesis of cuo/cu2o nanoparticles on cotton and viscose rayon fabrics
EP  - 813
IS  - 7-8
SP  - 805
VL  - 53
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4193
ER  - 

@article{
author = "Marković, Darka and Jokić, Bojan and Radovanović, Željko and Ašanin, Jelena and Radoičić, Marija B. and Mitrić, Miodrag and Mišić, Dušan and Radetić, Maja",
year = "2019",
abstract = "This study discusses the possibility of in situ synthesis of Cu-based nanoparticles (NPs) on cotton and viscose rayon fabrics previously modified with 1,2,3,4-butanetetracarboxylic acid (BTCA) of different concentrations, with the aim to obtain antibacterial protection. The changes in the chemical structure of the fibers caused by the modification with BTCA were assessed by FTIR spectroscopy. The higher the concentration of the applied BTCA, the larger the amounts of free carboxyl groups in both fabrics. AAS analysis showed that the samples modified with higher concentration of BTCA provided larger Cu2+-ions uptake and consequently, higher content of Cu after Cu2+-ions reduction. The presence of Cu-based NPs on the fabrics was confirmed by FESEM and EDS analyses. XRD analysis revealed that NPs appeared as a mixture of CuO and Cu2O. Synthesized NPs imparted excellent antibacterial activity to both fabrics against E. coli and S. aureus, including methicillin-resistant S. aureus.",
publisher = "Editura Acad Romane, Bucuresti",
journal = "Cellulose Chemistry and Technology",
title = "Influence of 1,2,3,4-butanetetracarboxylic acid concentration on in situ synthesis of cuo/cu2o nanoparticles on cotton and viscose rayon fabrics",
pages = "813-805",
number = "7-8",
volume = "53",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4193"
}

Marković, D., Jokić, B., Radovanović, Ž., Ašanin, J., Radoičić, M. B., Mitrić, M., Mišić, D.,& Radetić, M.. (2019). Influence of 1,2,3,4-butanetetracarboxylic acid concentration on in situ synthesis of cuo/cu2o nanoparticles on cotton and viscose rayon fabrics. in Cellulose Chemistry and Technology
Editura Acad Romane, Bucuresti., 53(7-8), 805-813.
https://hdl.handle.net/21.15107/rcub_technorep_4193

Marković D, Jokić B, Radovanović Ž, Ašanin J, Radoičić MB, Mitrić M, Mišić D, Radetić M. Influence of 1,2,3,4-butanetetracarboxylic acid concentration on in situ synthesis of cuo/cu2o nanoparticles on cotton and viscose rayon fabrics. in Cellulose Chemistry and Technology. 2019;53(7-8):805-813.
https://hdl.handle.net/21.15107/rcub_technorep_4193 .

Marković, Darka, Jokić, Bojan, Radovanović, Željko, Ašanin, Jelena, Radoičić, Marija B., Mitrić, Miodrag, Mišić, Dušan, Radetić, Maja, "Influence of 1,2,3,4-butanetetracarboxylic acid concentration on in situ synthesis of cuo/cu2o nanoparticles on cotton and viscose rayon fabrics" in Cellulose Chemistry and Technology, 53, no. 7-8 (2019):805-813,
https://hdl.handle.net/21.15107/rcub_technorep_4193 .

TY  - JOUR
AU  - Žižović, Irena
AU  - Senerović, Lidija
AU  - Morić, Ivana
AU  - Adamović, Tijana
AU  - Jovanović, Milena
AU  - Kalagasidis Krušić, Melina
AU  - Mišić, Dušan
AU  - Stojanović, Dušica
AU  - Milovanović, Stoja
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3923
AB  - This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 degrees C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus
EP  - 20
SP  - 11
VL  - 140
DO  - 10.1016/j.supflu.2018.05.025
ER  - 

@article{
author = "Žižović, Irena and Senerović, Lidija and Morić, Ivana and Adamović, Tijana and Jovanović, Milena and Kalagasidis Krušić, Melina and Mišić, Dušan and Stojanović, Dušica and Milovanović, Stoja",
year = "2018",
abstract = "This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 degrees C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus",
pages = "20-11",
volume = "140",
doi = "10.1016/j.supflu.2018.05.025"
}

Žižović, I., Senerović, L., Morić, I., Adamović, T., Jovanović, M., Kalagasidis Krušić, M., Mišić, D., Stojanović, D.,& Milovanović, S.. (2018). Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 140, 11-20.
https://doi.org/10.1016/j.supflu.2018.05.025

Žižović I, Senerović L, Morić I, Adamović T, Jovanović M, Kalagasidis Krušić M, Mišić D, Stojanović D, Milovanović S. Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in Journal of Supercritical Fluids. 2018;140:11-20.
doi:10.1016/j.supflu.2018.05.025 .

Žižović, Irena, Senerović, Lidija, Morić, Ivana, Adamović, Tijana, Jovanović, Milena, Kalagasidis Krušić, Melina, Mišić, Dušan, Stojanović, Dušica, Milovanović, Stoja, "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus" in Journal of Supercritical Fluids, 140 (2018):11-20,
https://doi.org/10.1016/j.supflu.2018.05.025 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Adamović, Tijana
AU  - Aksentijević, Ksenija
AU  - Mišić, Dušan
AU  - Ivanović, Jasna
AU  - Žižović, Irena
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3571
AB  - Supercritical CO2 was used as a green solvent and impregnation medium for loading cellulose acetate beads with carvacrol in order to obtain a biomaterial with antibacterial properties. Supercritical solvent impregnation was performed in a high-pressure view cell at temperature of 50 degrees C and pressures of 10, 21, and 30 MPa with the processing time ranging from 2 to 18 h. The rate of impregnation increased with the pressure increase. However, maximum impregnation yield (round 60%) was not affected by the pressure applied. Selected samples of the impregnated cellulose acetate containing 6-60% of carvacrol were proven to have considerable antibacterial effect against Gram-positive and Gram-negative bacterial strains including methicillin-resistant Staphylococcus aureus which causes severe infections in humans and animals. In addition, cellulose acetate beads containing 6.0-33.6% of carvacrol were shown to have a porous structure with submicron pores which is of interest for the controlled delivery applications.
PB  - Hindawi Ltd, London
T2  - International Journal of Polymer Science
T1  - Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation
VL  - 2017
DO  - 10.1155/2017/8762649
ER  - 

@article{
author = "Milovanović, Stoja and Adamović, Tijana and Aksentijević, Ksenija and Mišić, Dušan and Ivanović, Jasna and Žižović, Irena",
year = "2017",
abstract = "Supercritical CO2 was used as a green solvent and impregnation medium for loading cellulose acetate beads with carvacrol in order to obtain a biomaterial with antibacterial properties. Supercritical solvent impregnation was performed in a high-pressure view cell at temperature of 50 degrees C and pressures of 10, 21, and 30 MPa with the processing time ranging from 2 to 18 h. The rate of impregnation increased with the pressure increase. However, maximum impregnation yield (round 60%) was not affected by the pressure applied. Selected samples of the impregnated cellulose acetate containing 6-60% of carvacrol were proven to have considerable antibacterial effect against Gram-positive and Gram-negative bacterial strains including methicillin-resistant Staphylococcus aureus which causes severe infections in humans and animals. In addition, cellulose acetate beads containing 6.0-33.6% of carvacrol were shown to have a porous structure with submicron pores which is of interest for the controlled delivery applications.",
publisher = "Hindawi Ltd, London",
journal = "International Journal of Polymer Science",
title = "Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation",
volume = "2017",
doi = "10.1155/2017/8762649"
}

Milovanović, S., Adamović, T., Aksentijević, K., Mišić, D., Ivanović, J.,& Žižović, I.. (2017). Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation. in International Journal of Polymer Science
Hindawi Ltd, London., 2017.
https://doi.org/10.1155/2017/8762649

Milovanović S, Adamović T, Aksentijević K, Mišić D, Ivanović J, Žižović I. Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation. in International Journal of Polymer Science. 2017;2017.
doi:10.1155/2017/8762649 .

Milovanović, Stoja, Adamović, Tijana, Aksentijević, Ksenija, Mišić, Dušan, Ivanović, Jasna, Žižović, Irena, "Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation" in International Journal of Polymer Science, 2017 (2017),
https://doi.org/10.1155/2017/8762649 . .

TY  - JOUR
AU  - Aksentijević, Ksenija
AU  - Ašanin, Jelena
AU  - Milivojević, Dušan
AU  - Čolović, Svetlana
AU  - Butorac, Ana
AU  - Cindrić, Mario
AU  - Mišić, Dušan
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5811
AB  - For the purpose of precise antibiotic susceptibility testing it is necessary to clearly distinguish Pseudomonas and Stenotrophomonas genera, considering acquired resistance of Pseudomonas species, as well as the intrinsic resistance of Stenotrophomonas species. This is why in the identification of the 51 isolates originated from fish, the following methods were used: standard PCR, 16S rRNA gene sequencing, and MALDI-TOF. The results of the standard PCR test, 16S rRNA gene sequencing and MALDI-TOF analysis confirmed 35 strains to belong to the Pseudomonas genus. Standard PCR test and VITEK MS device confirmed that 10 strains belong to Stenotrophomonas maltophilia species. Three strains were positive in both standard PCR tests for Pseudomonas and Stenotrpohomonas. 16S rRNA gene sequencing identified these 3 strains to be 99% Pseudomonas sp. and 99% Stenotrophomonas sp. VITEK MS first identified these three strains as 99% Stenotrophomonas, and in the repeated identification it identified them as 99% Pseudomonas. MALDI TOF/TOF 4800 Plus device identified these strains as Stenotrophomonas. Three strains were negative in both standard PCR tests for Pseudomonas and Stenotrpohomonas. 16S rRNA gene sequencing identified these 3 strains to be 99% Pseudomonas sp. and 99% Stenotrophomonas sp. VITEK MS first identified these three strains as 99% Stenotrophomonas, and in the repeated identification it identified them as 99% Pseudomonas. MALDI TOF/TOF 4800 Plus device identified these strains as Stenotrophomonas. Although modern test methods that have very high specificity (PCR, 16S rRNA gene Pseudomonas and Stenotrophomonas species for 6 isolates could not be reached using the above mentioned methods.
AB  - S obzirom na značaj stečene rezistencije Pseudomonas vrsta, kao i na intrinzičnu rezistenciju Stenotrophomonas vrsta, a u cilju preciznog ispitivanja osetljivosti na antibiotike, neophodna je jasna diferencijacija pripadnika ovih rodova bakterija. U tom cilju su u identifikaciji 51 izolata poreklom od riba korišćene metode: standardni PCR, 16S rRNA sekvenciranje gena, MALDI-TOF. Rezultati standardnog PCR testa, 16S rRNA sekvenciranja gena i MALDI-TOF analize su za 35 sojeva potvrdili pripadnost rodu Pseudomonas. Standardnim PCR testom i primenom aparata VITEK MS utvrđeno je da 10 sojeva pripada vrsti Stenotrophomonas maltophilia. U 16S rRNA sekvenciranju gena 3 soja koja su bila pozitivna u oba standardna PCR testa identifikovana su kao 99% Pseudomonas sp. i 99% Stenotrophomonas sp. VITEK MS je ova tri soja u prvoj identifikaciji identifikovao kao 99% Stenotrophomonas, a u ponovljenoj identifikaciji kao 99% Pseudomonas. Ti sojevi su na aparatu MALDI TOF/TOF 4800 Plus bili identifikovani kao Stenotrophomonas. U 16S rRNA sekvenciranju gena tri soja koja su bila negativna u oba standardna PCR testa su identifikovana kao 99% Pseudomonas sp. i 99% Stenotrophomonas sp. Aparat VITEK MS je ova tri soja identifikovao u jednoj identifikaciji kao 99% Stenotrophomonas, a u drugoj identifikaciji 99% kao Pseudomonas. Ti sojevi su na aparatu MALDI TOF/ TOF 4800 Plus bili identifikovani kao Stenotrophomonas. Iako su u ovom istraživanju korišćene savremene metode ispitivanja koje imaju vrlo visoku specifičnost (PCR, 16s rRNK sequencing, MALDI TOF) precizna diferencijacija Pseudomonas i Stenotrophomonas vrsta nije mogla biti postignuta.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method
T1  - Diferencijacija Pseudomonas i Stenotrophomonas vrsta izolovanih iz riba primenom molekularnih metoda i MALDI-TOF metode
EP  - 316
IS  - 3
SP  - 304
VL  - 66
DO  - 10.1515/acve-2016-0027
ER  - 

@article{
author = "Aksentijević, Ksenija and Ašanin, Jelena and Milivojević, Dušan and Čolović, Svetlana and Butorac, Ana and Cindrić, Mario and Mišić, Dušan",
year = "2016",
abstract = "For the purpose of precise antibiotic susceptibility testing it is necessary to clearly distinguish Pseudomonas and Stenotrophomonas genera, considering acquired resistance of Pseudomonas species, as well as the intrinsic resistance of Stenotrophomonas species. This is why in the identification of the 51 isolates originated from fish, the following methods were used: standard PCR, 16S rRNA gene sequencing, and MALDI-TOF. The results of the standard PCR test, 16S rRNA gene sequencing and MALDI-TOF analysis confirmed 35 strains to belong to the Pseudomonas genus. Standard PCR test and VITEK MS device confirmed that 10 strains belong to Stenotrophomonas maltophilia species. Three strains were positive in both standard PCR tests for Pseudomonas and Stenotrpohomonas. 16S rRNA gene sequencing identified these 3 strains to be 99% Pseudomonas sp. and 99% Stenotrophomonas sp. VITEK MS first identified these three strains as 99% Stenotrophomonas, and in the repeated identification it identified them as 99% Pseudomonas. MALDI TOF/TOF 4800 Plus device identified these strains as Stenotrophomonas. Three strains were negative in both standard PCR tests for Pseudomonas and Stenotrpohomonas. 16S rRNA gene sequencing identified these 3 strains to be 99% Pseudomonas sp. and 99% Stenotrophomonas sp. VITEK MS first identified these three strains as 99% Stenotrophomonas, and in the repeated identification it identified them as 99% Pseudomonas. MALDI TOF/TOF 4800 Plus device identified these strains as Stenotrophomonas. Although modern test methods that have very high specificity (PCR, 16S rRNA gene Pseudomonas and Stenotrophomonas species for 6 isolates could not be reached using the above mentioned methods., S obzirom na značaj stečene rezistencije Pseudomonas vrsta, kao i na intrinzičnu rezistenciju Stenotrophomonas vrsta, a u cilju preciznog ispitivanja osetljivosti na antibiotike, neophodna je jasna diferencijacija pripadnika ovih rodova bakterija. U tom cilju su u identifikaciji 51 izolata poreklom od riba korišćene metode: standardni PCR, 16S rRNA sekvenciranje gena, MALDI-TOF. Rezultati standardnog PCR testa, 16S rRNA sekvenciranja gena i MALDI-TOF analize su za 35 sojeva potvrdili pripadnost rodu Pseudomonas. Standardnim PCR testom i primenom aparata VITEK MS utvrđeno je da 10 sojeva pripada vrsti Stenotrophomonas maltophilia. U 16S rRNA sekvenciranju gena 3 soja koja su bila pozitivna u oba standardna PCR testa identifikovana su kao 99% Pseudomonas sp. i 99% Stenotrophomonas sp. VITEK MS je ova tri soja u prvoj identifikaciji identifikovao kao 99% Stenotrophomonas, a u ponovljenoj identifikaciji kao 99% Pseudomonas. Ti sojevi su na aparatu MALDI TOF/TOF 4800 Plus bili identifikovani kao Stenotrophomonas. U 16S rRNA sekvenciranju gena tri soja koja su bila negativna u oba standardna PCR testa su identifikovana kao 99% Pseudomonas sp. i 99% Stenotrophomonas sp. Aparat VITEK MS je ova tri soja identifikovao u jednoj identifikaciji kao 99% Stenotrophomonas, a u drugoj identifikaciji 99% kao Pseudomonas. Ti sojevi su na aparatu MALDI TOF/ TOF 4800 Plus bili identifikovani kao Stenotrophomonas. Iako su u ovom istraživanju korišćene savremene metode ispitivanja koje imaju vrlo visoku specifičnost (PCR, 16s rRNK sequencing, MALDI TOF) precizna diferencijacija Pseudomonas i Stenotrophomonas vrsta nije mogla biti postignuta.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method, Diferencijacija Pseudomonas i Stenotrophomonas vrsta izolovanih iz riba primenom molekularnih metoda i MALDI-TOF metode",
pages = "316-304",
number = "3",
volume = "66",
doi = "10.1515/acve-2016-0027"
}

Aksentijević, K., Ašanin, J., Milivojević, D., Čolović, S., Butorac, A., Cindrić, M.,& Mišić, D.. (2016). Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3), 304-316.
https://doi.org/10.1515/acve-2016-0027

Aksentijević K, Ašanin J, Milivojević D, Čolović S, Butorac A, Cindrić M, Mišić D. Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method. in Acta Veterinaria-Beograd. 2016;66(3):304-316.
doi:10.1515/acve-2016-0027 .

Aksentijević, Ksenija, Ašanin, Jelena, Milivojević, Dušan, Čolović, Svetlana, Butorac, Ana, Cindrić, Mario, Mišić, Dušan, "Differentiation between Pseudomonas and Stenotrophomonas species isolated from fish using molecular and MALDI-TOF method" in Acta Veterinaria-Beograd, 66, no. 3 (2016):304-316,
https://doi.org/10.1515/acve-2016-0027 . .

TY  - JOUR
AU  - Roj, Edward
AU  - Tadić, Vanja M.
AU  - Mišić, Dušan
AU  - Žižović, Irena
AU  - Arsić, Ivana
AU  - Dobrzynska-Inger, Agnieszka
AU  - Kostrzewa, Dorota
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5797
AB  - Extracts obtained from hops (Humulus lupulus L., Cannabaceae) by supercritical fluid extraction (SFE), SFE followed by isomerization, as well as by conventional technique, were investigated for their chemical composition and antibacterial activity against selected foodborne pathogens and microorganisms capable to cause the food spoilage. The antibacterial activity of the extracts was compared with the antibacterial activity of xanthohumol, compound known for its broad pharmacological properties, isolated from the raw material remained after the SFE. Xanthohumol (XH, 96%) proved to posses the most prominent activity against all the tested strains, with the MIC values ranged between 2.5 and 20 mu g mL(-1). Supercritical hops extract and potassium isomerized supercritical hops extract showed strong antibacterial activity against the tested strains as well. Escherichia coli was not affected by the extracts, meaning that their oral admission would not cause the same problem as antibiotic application in intestinal flora. The chemical composition of the investigated hops extracts was analysed by GC-MS. Contents of a-acids, beta-acids, iso-alpha-acids and xanthohumol in the samples were determined by HPLC.
PB  - De Gruyter Open Ltd, Warsaw
T2  - Open Chemistry
T1  - Supercritical carbon dioxide hops extracts with antimicrobial properties
EP  - 1171
IS  - 1
SP  - 1157
VL  - 13
DO  - 10.1515/chem-2015-0131
ER  - 

@article{
author = "Roj, Edward and Tadić, Vanja M. and Mišić, Dušan and Žižović, Irena and Arsić, Ivana and Dobrzynska-Inger, Agnieszka and Kostrzewa, Dorota",
year = "2015",
abstract = "Extracts obtained from hops (Humulus lupulus L., Cannabaceae) by supercritical fluid extraction (SFE), SFE followed by isomerization, as well as by conventional technique, were investigated for their chemical composition and antibacterial activity against selected foodborne pathogens and microorganisms capable to cause the food spoilage. The antibacterial activity of the extracts was compared with the antibacterial activity of xanthohumol, compound known for its broad pharmacological properties, isolated from the raw material remained after the SFE. Xanthohumol (XH, 96%) proved to posses the most prominent activity against all the tested strains, with the MIC values ranged between 2.5 and 20 mu g mL(-1). Supercritical hops extract and potassium isomerized supercritical hops extract showed strong antibacterial activity against the tested strains as well. Escherichia coli was not affected by the extracts, meaning that their oral admission would not cause the same problem as antibiotic application in intestinal flora. The chemical composition of the investigated hops extracts was analysed by GC-MS. Contents of a-acids, beta-acids, iso-alpha-acids and xanthohumol in the samples were determined by HPLC.",
publisher = "De Gruyter Open Ltd, Warsaw",
journal = "Open Chemistry",
title = "Supercritical carbon dioxide hops extracts with antimicrobial properties",
pages = "1171-1157",
number = "1",
volume = "13",
doi = "10.1515/chem-2015-0131"
}

Roj, E., Tadić, V. M., Mišić, D., Žižović, I., Arsić, I., Dobrzynska-Inger, A.,& Kostrzewa, D.. (2015). Supercritical carbon dioxide hops extracts with antimicrobial properties. in Open Chemistry
De Gruyter Open Ltd, Warsaw., 13(1), 1157-1171.
https://doi.org/10.1515/chem-2015-0131

Roj E, Tadić VM, Mišić D, Žižović I, Arsić I, Dobrzynska-Inger A, Kostrzewa D. Supercritical carbon dioxide hops extracts with antimicrobial properties. in Open Chemistry. 2015;13(1):1157-1171.
doi:10.1515/chem-2015-0131 .

Roj, Edward, Tadić, Vanja M., Mišić, Dušan, Žižović, Irena, Arsić, Ivana, Dobrzynska-Inger, Agnieszka, Kostrzewa, Dorota, "Supercritical carbon dioxide hops extracts with antimicrobial properties" in Open Chemistry, 13, no. 1 (2015):1157-1171,
https://doi.org/10.1515/chem-2015-0131 . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Lukić, Milica Z.
AU  - Tasić-Kostov, Marija Z.
AU  - Tadić, Vanja
AU  - Arsić, Ivana A.
AU  - Mišić, Dušan
AU  - Petrović, Slobodan
AU  - Savić, Snežana D.
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2883
AB  - Antimicrobial activity of Usnea barbata especially against bacteria involved in pathogenesis of various skin conditions has been well documented in literature. Nevertheless, there are no papers dealing with formulation of its isolates into topical preparations for treatment of skin infections. In present study, alkyl polyglucoside (APG)-based vehicle was developed as carrier of U. barbata CO2-supercritical extract (U-SE) that demonstrated the best anti- microbial potential in preliminary screening. For comparison, chosen extract in the same concentration and using the same procedure was incorporated into a pharmacopoeial vehicle. Comparative evaluation of physicochemical stability, efficiency and safety proved APG-based vehicle to possess certain preferential features as carrier of U-SE compared to the reference one, composing a topical formulation with potential clinical relevance in treatment of skin infections.
AB  - Antimikrobna aktivnost vrste Usnea barbata naročito protiv bakterija koje učestvuju u patogenezi različitih bolesti kože, dobro je dokumentovana u naučnoj literaturi. Uprkos tome, ne postoje radovi koji se bave formulacijom topikalnih preparata na bazi ovog lišaja namenjenih lečenju kožnih infekcija. U ovoj studiji, razvijena je podloga stablizovana alkil poliglukozidnim (APG) emulgatorom, kao potencijalni nosač za ekstrakt vrste U. barbata koji je pokazao najbolji antimikrobni potencijal u preliminarnom istraživanju nekoliko ekstrakata dobijenih upotrebom različitih ekstragenasa/postupaka. Radi poređenja, odabrani CO2-natkritični ekstrakt je inkorporiran u istoj koncentraciji (koja odgovara 2 mas.% usninske kiseline) i istim postupkom izrade u često korišćenu podlogu farmakopejskog kvaliteta, a zatim je sprovedeno uporedno istraživanje fizičko-hemijske stabilnosti, efikasnosti i bezbednosti na obe grupe uzoraka. Rezulati našeg istraživanja pokazali su da se razvijena podloga stabilizovana APG emulgatorom može smatrati pogodnim nosačem za CO2-natkritični ekstrakt vrste U. barbata, čineći topikalnu formulaciju sa potencijalnim kliničkim značajem u terapiji kožnih infekcija. Reološka istraživanja pokazala su zadovoljavajuću sposobnost emulzije stabilizovane APG emulgatorom da ostane fizički stabilna nakon dodatka CO2-natkritičnog ekstrakta vrste U. barbata, u poređenju sa farmakopejskom. Međutim, konduktometrijska merenja su pokazala određena neslaganja sa reološkim nala- zima, namećući potrebu za dodatnim istraživanjima radi konačne procene fizičke stabilnosti testiranih emulzionih sistema. Izmerene vrednosti pH tokom perioda od 90 dana čuvanja uzoraka na sobnoj temperaturi ukazale su na zadovoljavajuću preliminarnu hemijsku stabilnost svih uzoraka. Dalja istraživanja efikasnosti i bezbednosti pokazala su bolji antimikrobni potencijal uzoraka sa podlogom stabilizovanom APG emulgatorom u odnosu na tradicionalno upotrebljavanu farmakopejsku bazu poznatog kvaliteta. Zadovoljavajući preliminarni bezbedonosni profili su pokazani kako za uzorke stabilizovane APG emulgatorom tako i za referentne krem uzorke. Na osnovu navedenog, može se zaključiti da je istraživana podloga bazirana na APG emulgatoru pokazala određene povoljnije karakteristike kao nosač za CO2-natkritični ekstrakt vrste U. barbata u poređenju sa konvencionalno korišćenom podlogom, naročito uzimajući u obzir njene poboljšane senzorne karakteristike, kao osobinu koja je često odlučujuća za komplijansu pacijenata.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation
T1  - Emulzija stabilizovana alkil poliglukozidnim emulgatorom kao potencijalni nosač za CO2-natkritični ekstrakt vrste Usnea barbata - procena stabilnosti, bezbednosti i efikasnosti topikalne formulacije
EP  - 712
IS  - 6
SP  - 703
VL  - 69
DO  - 10.2298/HEMIND140701002Z
ER  - 

@article{
author = "Žugić, Ana R. and Lukić, Milica Z. and Tasić-Kostov, Marija Z. and Tadić, Vanja and Arsić, Ivana A. and Mišić, Dušan and Petrović, Slobodan and Savić, Snežana D.",
year = "2015",
abstract = "Antimicrobial activity of Usnea barbata especially against bacteria involved in pathogenesis of various skin conditions has been well documented in literature. Nevertheless, there are no papers dealing with formulation of its isolates into topical preparations for treatment of skin infections. In present study, alkyl polyglucoside (APG)-based vehicle was developed as carrier of U. barbata CO2-supercritical extract (U-SE) that demonstrated the best anti- microbial potential in preliminary screening. For comparison, chosen extract in the same concentration and using the same procedure was incorporated into a pharmacopoeial vehicle. Comparative evaluation of physicochemical stability, efficiency and safety proved APG-based vehicle to possess certain preferential features as carrier of U-SE compared to the reference one, composing a topical formulation with potential clinical relevance in treatment of skin infections., Antimikrobna aktivnost vrste Usnea barbata naročito protiv bakterija koje učestvuju u patogenezi različitih bolesti kože, dobro je dokumentovana u naučnoj literaturi. Uprkos tome, ne postoje radovi koji se bave formulacijom topikalnih preparata na bazi ovog lišaja namenjenih lečenju kožnih infekcija. U ovoj studiji, razvijena je podloga stablizovana alkil poliglukozidnim (APG) emulgatorom, kao potencijalni nosač za ekstrakt vrste U. barbata koji je pokazao najbolji antimikrobni potencijal u preliminarnom istraživanju nekoliko ekstrakata dobijenih upotrebom različitih ekstragenasa/postupaka. Radi poređenja, odabrani CO2-natkritični ekstrakt je inkorporiran u istoj koncentraciji (koja odgovara 2 mas.% usninske kiseline) i istim postupkom izrade u često korišćenu podlogu farmakopejskog kvaliteta, a zatim je sprovedeno uporedno istraživanje fizičko-hemijske stabilnosti, efikasnosti i bezbednosti na obe grupe uzoraka. Rezulati našeg istraživanja pokazali su da se razvijena podloga stabilizovana APG emulgatorom može smatrati pogodnim nosačem za CO2-natkritični ekstrakt vrste U. barbata, čineći topikalnu formulaciju sa potencijalnim kliničkim značajem u terapiji kožnih infekcija. Reološka istraživanja pokazala su zadovoljavajuću sposobnost emulzije stabilizovane APG emulgatorom da ostane fizički stabilna nakon dodatka CO2-natkritičnog ekstrakta vrste U. barbata, u poređenju sa farmakopejskom. Međutim, konduktometrijska merenja su pokazala određena neslaganja sa reološkim nala- zima, namećući potrebu za dodatnim istraživanjima radi konačne procene fizičke stabilnosti testiranih emulzionih sistema. Izmerene vrednosti pH tokom perioda od 90 dana čuvanja uzoraka na sobnoj temperaturi ukazale su na zadovoljavajuću preliminarnu hemijsku stabilnost svih uzoraka. Dalja istraživanja efikasnosti i bezbednosti pokazala su bolji antimikrobni potencijal uzoraka sa podlogom stabilizovanom APG emulgatorom u odnosu na tradicionalno upotrebljavanu farmakopejsku bazu poznatog kvaliteta. Zadovoljavajući preliminarni bezbedonosni profili su pokazani kako za uzorke stabilizovane APG emulgatorom tako i za referentne krem uzorke. Na osnovu navedenog, može se zaključiti da je istraživana podloga bazirana na APG emulgatoru pokazala određene povoljnije karakteristike kao nosač za CO2-natkritični ekstrakt vrste U. barbata u poređenju sa konvencionalno korišćenom podlogom, naročito uzimajući u obzir njene poboljšane senzorne karakteristike, kao osobinu koja je često odlučujuća za komplijansu pacijenata.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation, Emulzija stabilizovana alkil poliglukozidnim emulgatorom kao potencijalni nosač za CO2-natkritični ekstrakt vrste Usnea barbata - procena stabilnosti, bezbednosti i efikasnosti topikalne formulacije",
pages = "712-703",
number = "6",
volume = "69",
doi = "10.2298/HEMIND140701002Z"
}

Žugić, A. R., Lukić, M. Z., Tasić-Kostov, M. Z., Tadić, V., Arsić, I. A., Mišić, D., Petrović, S.,& Savić, S. D.. (2015). Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation. in Hemijska industrija
Association of Chemical Engineers of Serbia., 69(6), 703-712.
https://doi.org/10.2298/HEMIND140701002Z

Žugić AR, Lukić MZ, Tasić-Kostov MZ, Tadić V, Arsić IA, Mišić D, Petrović S, Savić SD. Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation. in Hemijska industrija. 2015;69(6):703-712.
doi:10.2298/HEMIND140701002Z .

Žugić, Ana R., Lukić, Milica Z., Tasić-Kostov, Marija Z., Tadić, Vanja, Arsić, Ivana A., Mišić, Dušan, Petrović, Slobodan, Savić, Snežana D., "Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation" in Hemijska industrija, 69, no. 6 (2015):703-712,
https://doi.org/10.2298/HEMIND140701002Z . .

TY  - JOUR
AU  - Stamenić, Marko
AU  - Vulić, Jelena
AU  - Đilas, Sonja
AU  - Mišić, Dušan
AU  - Tadić, Vanja
AU  - Petrović, Slobodan
AU  - Žižović, Irena
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2655
AB  - The antioxidant and antibacterial properties of Greek oregano extracts obtained by fractional supercritical fluid extraction (SFE) with carbon dioxide were investigated and compared with the properties of essential oil obtained by hydrodistillation. According to DPPH., hydroxyl radical and superoxide anion radical scavenging activity assays, the supercritical extracts expressed stronger antioxidant activity comparing to the essential oil. The most effective was the supercritical extract obtained by fractional extraction at 30 MPa and 100 degrees C after the volatile fraction had been extracted at lower pressure. At the same time this extract showed strong antibacterial activity against staphylococci, including MASA strain, but did not affect Escherichia coli of normal intestinal flora. The essential oil obtained by hydrodistillation showed stronger antibacterial activity against E. coli, Salmonella and Klebsiella pneumoniae, comparing to the supercritical extracts but at the same affected the normal gut flora.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE
EP  - 315
SP  - 307
VL  - 165
DO  - 10.1016/j.foodchem.2014.05.091
ER  - 

@article{
author = "Stamenić, Marko and Vulić, Jelena and Đilas, Sonja and Mišić, Dušan and Tadić, Vanja and Petrović, Slobodan and Žižović, Irena",
year = "2014",
abstract = "The antioxidant and antibacterial properties of Greek oregano extracts obtained by fractional supercritical fluid extraction (SFE) with carbon dioxide were investigated and compared with the properties of essential oil obtained by hydrodistillation. According to DPPH., hydroxyl radical and superoxide anion radical scavenging activity assays, the supercritical extracts expressed stronger antioxidant activity comparing to the essential oil. The most effective was the supercritical extract obtained by fractional extraction at 30 MPa and 100 degrees C after the volatile fraction had been extracted at lower pressure. At the same time this extract showed strong antibacterial activity against staphylococci, including MASA strain, but did not affect Escherichia coli of normal intestinal flora. The essential oil obtained by hydrodistillation showed stronger antibacterial activity against E. coli, Salmonella and Klebsiella pneumoniae, comparing to the supercritical extracts but at the same affected the normal gut flora.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE",
pages = "315-307",
volume = "165",
doi = "10.1016/j.foodchem.2014.05.091"
}

Stamenić, M., Vulić, J., Đilas, S., Mišić, D., Tadić, V., Petrović, S.,& Žižović, I.. (2014). Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE. in Food Chemistry
Elsevier Sci Ltd, Oxford., 165, 307-315.
https://doi.org/10.1016/j.foodchem.2014.05.091

Stamenić M, Vulić J, Đilas S, Mišić D, Tadić V, Petrović S, Žižović I. Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE. in Food Chemistry. 2014;165:307-315.
doi:10.1016/j.foodchem.2014.05.091 .

Stamenić, Marko, Vulić, Jelena, Đilas, Sonja, Mišić, Dušan, Tadić, Vanja, Petrović, Slobodan, Žižović, Irena, "Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE" in Food Chemistry, 165 (2014):307-315,
https://doi.org/10.1016/j.foodchem.2014.05.091 . .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Dimitrijević-Branković, Suzana
AU  - Mišić, Dušan
AU  - Ristić, Mihailo S.
AU  - Žižović, Irena
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2507
AB  - For the first time, extracts from clove buds obtained by supercritical carbon dioxide extraction were screened for antioxidant and antibacterial activities. Additionally, antioxidant and antibacterial activities of extracts obtained by the supercritical extraction of the clove bud-oregano leaf mixtures were studied. Supercritical extract of pure clove had the highest eugenol (64%) and total phenolic content (530.56 mg GAE/g(extract)). All extracts had antioxidant activity comparable to synthetic antioxidants against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and formation of peroxides. Presence of 0.6% and 5% of oregano extract in the clove extracts obtained from the clove-oregano plant mixtures improved their antioxidant activity with respect to the extract from pure clove. Clove extract showed moderate antibacterial activities against selected Staphylococcus and Enterococcus bacterial strains. Presence of 50% of the oregano extract improved antibacterial activity of clove extract against all tested strains and resulted in a synergistic antibacterial activity against Methicillin-resistant Staphylococcus haemolyticus strain (MIC  lt = 1.25 mu g/mL). Study demonstrated great potential of supercritical clove extract as natural functional ingredient and the possibility of increasing its antioxidant and antibacterial efficiencies in order to apply lower concentrations and to reduce undesirable flavour notes and toxicological effects in final products.
PB  - Elsevier, Amsterdam
T2  - Journal of Functional Foods
T1  - Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds
EP  - 423
IS  - 1
SP  - 416
VL  - 5
DO  - 10.1016/j.jff.2012.11.014
ER  - 

@article{
author = "Ivanović, Jasna and Dimitrijević-Branković, Suzana and Mišić, Dušan and Ristić, Mihailo S. and Žižović, Irena",
year = "2013",
abstract = "For the first time, extracts from clove buds obtained by supercritical carbon dioxide extraction were screened for antioxidant and antibacterial activities. Additionally, antioxidant and antibacterial activities of extracts obtained by the supercritical extraction of the clove bud-oregano leaf mixtures were studied. Supercritical extract of pure clove had the highest eugenol (64%) and total phenolic content (530.56 mg GAE/g(extract)). All extracts had antioxidant activity comparable to synthetic antioxidants against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and formation of peroxides. Presence of 0.6% and 5% of oregano extract in the clove extracts obtained from the clove-oregano plant mixtures improved their antioxidant activity with respect to the extract from pure clove. Clove extract showed moderate antibacterial activities against selected Staphylococcus and Enterococcus bacterial strains. Presence of 50% of the oregano extract improved antibacterial activity of clove extract against all tested strains and resulted in a synergistic antibacterial activity against Methicillin-resistant Staphylococcus haemolyticus strain (MIC  lt = 1.25 mu g/mL). Study demonstrated great potential of supercritical clove extract as natural functional ingredient and the possibility of increasing its antioxidant and antibacterial efficiencies in order to apply lower concentrations and to reduce undesirable flavour notes and toxicological effects in final products.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Functional Foods",
title = "Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds",
pages = "423-416",
number = "1",
volume = "5",
doi = "10.1016/j.jff.2012.11.014"
}

Ivanović, J., Dimitrijević-Branković, S., Mišić, D., Ristić, M. S.,& Žižović, I.. (2013). Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds. in Journal of Functional Foods
Elsevier, Amsterdam., 5(1), 416-423.
https://doi.org/10.1016/j.jff.2012.11.014

Ivanović J, Dimitrijević-Branković S, Mišić D, Ristić MS, Žižović I. Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds. in Journal of Functional Foods. 2013;5(1):416-423.
doi:10.1016/j.jff.2012.11.014 .

Ivanović, Jasna, Dimitrijević-Branković, Suzana, Mišić, Dušan, Ristić, Mihailo S., Žižović, Irena, "Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds" in Journal of Functional Foods, 5, no. 1 (2013):416-423,
https://doi.org/10.1016/j.jff.2012.11.014 . .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Meyer, Florian
AU  - Mišić, Dušan
AU  - Ašanin, Jelena
AU  - Jaeger, Philip
AU  - Žižović, Irena
AU  - Eggers, Rudolf
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5700
AB  - Multiplication and spreading of multiresistant pathogenic bacteria that cause fatal infections in humans and animals have boosted research on implementation of newer technologies for producing natural substances with strong antibacterial activities. In this study, SFE from lichen Usnea barbata was investigated for different pre-treatment methods with respect to extracts yield, usnic acid recovery and antibacterial activity against more than thirty methicillin resistant staphylococci strains. Extraction of U. barbata with subcritical carbon dioxide was studied as well. Extraction yield, usnic acid content and antibacterial activity of the extracts were strongly affected by the pre-treatment method. Intensive pre-treatment methods such as flaking and cutting + grinding caused a considerable comminution of the lichen thallus and enabled high extraction yields. On the other hand, mild pre-treatment of the lichen such as impact + shearing enabled isolation of extracts with lower MIC values. Experimental results indicated that extraction with liquid carbon dioxide at 30 MPa and 25 degrees C was the most favorable condition at which a satisfactory high extraction yield was obtained (2.08%). Extract isolated with liquid carbon dioxide had a high usnic acid content in the extract (632 g/kg) and a very strong antibacterial activity (average MIC of 19.11 mu g/mL). Antibacterial activity of all the extracts against tested strains was comparable to the pure usnic acid or even stronger. This study indicated a potential use of the supercritical extracts of U. barbata in pharmaceutical formulations for treatment of infections caused by staphylococci.
PB  - Elsevier, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent
EP  - 9
SP  - 1
VL  - 76
DO  - 10.1016/j.supflu.2013.01.005
ER  - 

@article{
author = "Ivanović, Jasna and Meyer, Florian and Mišić, Dušan and Ašanin, Jelena and Jaeger, Philip and Žižović, Irena and Eggers, Rudolf",
year = "2013",
abstract = "Multiplication and spreading of multiresistant pathogenic bacteria that cause fatal infections in humans and animals have boosted research on implementation of newer technologies for producing natural substances with strong antibacterial activities. In this study, SFE from lichen Usnea barbata was investigated for different pre-treatment methods with respect to extracts yield, usnic acid recovery and antibacterial activity against more than thirty methicillin resistant staphylococci strains. Extraction of U. barbata with subcritical carbon dioxide was studied as well. Extraction yield, usnic acid content and antibacterial activity of the extracts were strongly affected by the pre-treatment method. Intensive pre-treatment methods such as flaking and cutting + grinding caused a considerable comminution of the lichen thallus and enabled high extraction yields. On the other hand, mild pre-treatment of the lichen such as impact + shearing enabled isolation of extracts with lower MIC values. Experimental results indicated that extraction with liquid carbon dioxide at 30 MPa and 25 degrees C was the most favorable condition at which a satisfactory high extraction yield was obtained (2.08%). Extract isolated with liquid carbon dioxide had a high usnic acid content in the extract (632 g/kg) and a very strong antibacterial activity (average MIC of 19.11 mu g/mL). Antibacterial activity of all the extracts against tested strains was comparable to the pure usnic acid or even stronger. This study indicated a potential use of the supercritical extracts of U. barbata in pharmaceutical formulations for treatment of infections caused by staphylococci.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent",
pages = "9-1",
volume = "76",
doi = "10.1016/j.supflu.2013.01.005"
}

Ivanović, J., Meyer, F., Mišić, D., Ašanin, J., Jaeger, P., Žižović, I.,& Eggers, R.. (2013). Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent. in Journal of Supercritical Fluids
Elsevier, Amsterdam., 76, 1-9.
https://doi.org/10.1016/j.supflu.2013.01.005

Ivanović J, Meyer F, Mišić D, Ašanin J, Jaeger P, Žižović I, Eggers R. Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent. in Journal of Supercritical Fluids. 2013;76:1-9.
doi:10.1016/j.supflu.2013.01.005 .

Ivanović, Jasna, Meyer, Florian, Mišić, Dušan, Ašanin, Jelena, Jaeger, Philip, Žižović, Irena, Eggers, Rudolf, "Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent" in Journal of Supercritical Fluids, 76 (2013):1-9,
https://doi.org/10.1016/j.supflu.2013.01.005 . .

TY  - JOUR
AU  - Žižović, Irena
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Stamenić, Marko
AU  - Đorđević, Sofija
AU  - Kukić-Marković, Jelena
AU  - Petrović, Slobodan
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2123
AB  - The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.
EP  - 14
SP  - 7
VL  - 72
DO  - 10.1016/j.supflu.2012.07.018
ER  - 

@article{
author = "Žižović, Irena and Ivanović, Jasna and Mišić, Dušan and Stamenić, Marko and Đorđević, Sofija and Kukić-Marković, Jelena and Petrović, Slobodan",
year = "2012",
abstract = "The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.",
pages = "14-7",
volume = "72",
doi = "10.1016/j.supflu.2012.07.018"
}

Žižović, I., Ivanović, J., Mišić, D., Stamenić, M., Đorđević, S., Kukić-Marković, J.,& Petrović, S.. (2012). SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 72, 7-14.
https://doi.org/10.1016/j.supflu.2012.07.018

Žižović I, Ivanović J, Mišić D, Stamenić M, Đorđević S, Kukić-Marković J, Petrović S. SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids. 2012;72:7-14.
doi:10.1016/j.supflu.2012.07.018 .

Žižović, Irena, Ivanović, Jasna, Mišić, Dušan, Stamenić, Marko, Đorđević, Sofija, Kukić-Marković, Jelena, Petrović, Slobodan, "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L." in Journal of Supercritical Fluids, 72 (2012):7-14,
https://doi.org/10.1016/j.supflu.2012.07.018 . .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Žižović, Irena
AU  - Ristić, Mihailo
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5691
AB  - Antibacterial activity of thyme, rosemary and sage isolates obtained by supercritical fluid extraction and hydrodistillation was investigated on Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis and Escherichia coli strains. Bacillus species were the most susceptible to all tested isolates. The thyme isolates showed the strongest antibacterial activity against tested bacteria with MIC values of 40-640 mu g/ml, followed by rosemary (MIC = 320-1280 mu g/ml) and sage (MIC = 160-2560 mu g/ml) isolates. Therefore, the antibacterial activity of the most abundant components found in the thyme isolates, thymol, p-cymene and their mixture was investigated as well. The thyme isolates, especially supercritical extract, showed stronger antibacterial activity against Bacillus strains compared to the single components and their mixture, which indicated synergetic effect of the other components. Results of this study indicated thyme as a valuable source of natural antibacterial agents and supercritical fluid extraction as an efficient isolation method.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Control
T1  - In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates
EP  - 116
IS  - 1
SP  - 110
VL  - 25
DO  - 10.1016/j.foodcont.2011.10.019
ER  - 

@article{
author = "Ivanović, Jasna and Mišić, Dušan and Žižović, Irena and Ristić, Mihailo",
year = "2012",
abstract = "Antibacterial activity of thyme, rosemary and sage isolates obtained by supercritical fluid extraction and hydrodistillation was investigated on Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis and Escherichia coli strains. Bacillus species were the most susceptible to all tested isolates. The thyme isolates showed the strongest antibacterial activity against tested bacteria with MIC values of 40-640 mu g/ml, followed by rosemary (MIC = 320-1280 mu g/ml) and sage (MIC = 160-2560 mu g/ml) isolates. Therefore, the antibacterial activity of the most abundant components found in the thyme isolates, thymol, p-cymene and their mixture was investigated as well. The thyme isolates, especially supercritical extract, showed stronger antibacterial activity against Bacillus strains compared to the single components and their mixture, which indicated synergetic effect of the other components. Results of this study indicated thyme as a valuable source of natural antibacterial agents and supercritical fluid extraction as an efficient isolation method.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Control",
title = "In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates",
pages = "116-110",
number = "1",
volume = "25",
doi = "10.1016/j.foodcont.2011.10.019"
}

Ivanović, J., Mišić, D., Žižović, I.,& Ristić, M.. (2012). In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates. in Food Control
Elsevier Sci Ltd, Oxford., 25(1), 110-116.
https://doi.org/10.1016/j.foodcont.2011.10.019

Ivanović J, Mišić D, Žižović I, Ristić M. In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates. in Food Control. 2012;25(1):110-116.
doi:10.1016/j.foodcont.2011.10.019 .

Ivanović, Jasna, Mišić, Dušan, Žižović, Irena, Ristić, Mihailo, "In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates" in Food Control, 25, no. 1 (2012):110-116,
https://doi.org/10.1016/j.foodcont.2011.10.019 . .

TY  - JOUR
AU  - Stanojković, Aleksandar
AU  - Ašanin, Ružica
AU  - Mišić, Dušan
AU  - Ašanin, Jelena
AU  - Stanojkovic-Sebić, Aleksandra
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5747
AB  - Streptococcus suis is a normal inhabitant of the respiratory system of pigs. Clinically healthy pigs are a major reservoir of the pathogen, but, as well, the most important link in the epidemiology of infection in humans. Hence, the aim of this study was to establish the presence of S. suis on some pig farms in Serbia and to determine their serotype affiliation. For this research, we tested 226 samples: nasopharyngeal tonsil swabs, nasal cavities swabs, parts of or all nasopharyngeal tonsils, swabs from dead animals, swabs from pig carcasses, and swabs from the butchers knives. Identification of S. suis strains was performed with antisera specific for capsular antigens. S. suis serotype 2 was established in 67.7% cases, and serotypes 7, 9 and 1 were isolated in a much smaller percentage (17.6, 8.8 and 5.9%, respectively). Serotype 2 is the only serotype isolated from all the collected samples. Serotype 9 was isolated from healthy pig tonsil swab and from 2 nasal swabs, and was not found in the samples taken from diseased pigs. The serotype 1 was isolated only from nasopharyngeal tonsil swabs of piglets, while serotype 7 was isolated from nasopharyngeal tonsil swabs, nasopharyngeal tonsil sections, but also from nasal swabs of healthy pigs. It can be concluded that the infections caused by different serotypes of S. suis are present in the herd of pigs in Serbia; hence, it is very important to identify what serotypes of S. suis are involved in pathogenesis of infections in pigs.
PB  - Parlar Scientific Publications (P S P), Freising
T2  - Fresenius Environmental Bulletin
T1  - The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia
EP  - 3561
IS  - 11C
SP  - 3558
VL  - 21
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_868
ER  - 

@article{
author = "Stanojković, Aleksandar and Ašanin, Ružica and Mišić, Dušan and Ašanin, Jelena and Stanojkovic-Sebić, Aleksandra",
year = "2012",
abstract = "Streptococcus suis is a normal inhabitant of the respiratory system of pigs. Clinically healthy pigs are a major reservoir of the pathogen, but, as well, the most important link in the epidemiology of infection in humans. Hence, the aim of this study was to establish the presence of S. suis on some pig farms in Serbia and to determine their serotype affiliation. For this research, we tested 226 samples: nasopharyngeal tonsil swabs, nasal cavities swabs, parts of or all nasopharyngeal tonsils, swabs from dead animals, swabs from pig carcasses, and swabs from the butchers knives. Identification of S. suis strains was performed with antisera specific for capsular antigens. S. suis serotype 2 was established in 67.7% cases, and serotypes 7, 9 and 1 were isolated in a much smaller percentage (17.6, 8.8 and 5.9%, respectively). Serotype 2 is the only serotype isolated from all the collected samples. Serotype 9 was isolated from healthy pig tonsil swab and from 2 nasal swabs, and was not found in the samples taken from diseased pigs. The serotype 1 was isolated only from nasopharyngeal tonsil swabs of piglets, while serotype 7 was isolated from nasopharyngeal tonsil swabs, nasopharyngeal tonsil sections, but also from nasal swabs of healthy pigs. It can be concluded that the infections caused by different serotypes of S. suis are present in the herd of pigs in Serbia; hence, it is very important to identify what serotypes of S. suis are involved in pathogenesis of infections in pigs.",
publisher = "Parlar Scientific Publications (P S P), Freising",
journal = "Fresenius Environmental Bulletin",
title = "The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia",
pages = "3561-3558",
number = "11C",
volume = "21",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_868"
}

Stanojković, A., Ašanin, R., Mišić, D., Ašanin, J.,& Stanojkovic-Sebić, A.. (2012). The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia. in Fresenius Environmental Bulletin
Parlar Scientific Publications (P S P), Freising., 21(11C), 3558-3561.
https://hdl.handle.net/21.15107/rcub_veterinar_868

Stanojković A, Ašanin R, Mišić D, Ašanin J, Stanojkovic-Sebić A. The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia. in Fresenius Environmental Bulletin. 2012;21(11C):3558-3561.
https://hdl.handle.net/21.15107/rcub_veterinar_868 .

Stanojković, Aleksandar, Ašanin, Ružica, Mišić, Dušan, Ašanin, Jelena, Stanojkovic-Sebić, Aleksandra, "The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia" in Fresenius Environmental Bulletin, 21, no. 11C (2012):3558-3561,
https://hdl.handle.net/21.15107/rcub_veterinar_868 .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Ristić, Mihailo
AU  - Pešić, Olivera
AU  - Žižović, Irena
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5614
AB  - The present study deals with the supercritical carbon dioxide (SC-CO2) extraction and hydrodistillation (HD) of dried bay leaves (Laurus nobilis L.). The chemical composition and antibacterial activity of the SC-CO2 extract and essential oil (EO) from dried leaves of bay were compared to each other and literature data. Qualitative and quantitative analyzes of the SC-CO2 extract and EO were performed using GC-FID and GC-MS analytical methods. A significant difference in the chemical composition of the SC-CO2 extract and EO was observed. The EO comprised high contents of monoterpenes and their oxygenated derivates (98.4 %), principally 1,8-cineole (33.4 %), linalool (16.0 %) and α-terpinyl acetate (13.8 %), sabinene (6.91 %) and methyl eugenol (5.32 %). The SC-CO2 extract comprised twice less monoterpenes and their oxygenated derivates (43.89 %), together with sesquiterpenes (12.43 %), diterpenes (1.33 %) and esters (31.13 %). The major components were methyl linoleate (16.18 %), α-terpinyl acetate (12.88 %), linalool (9.00 %), methyl eugenol (8.67 %), methyl arachidonate (6.28 %) and eugenol (6.14 %). An investigation of the antibacterial activity of bay SC-CO2 extract and EO was completed on different Staphylococcus strains using the broth macrodilution method. Staphylococcus intermedius strains were the most susceptible to both the SC-CO2 extract and EO (MIC = 640 μg/ml).
AB  - U radu je ispitana nadkritična ekstrakcija i hidrodestilacija osušenih listova lovora (Laurus nobilis L.). Prikazana je uporedna analiza hemijskog sastava i antibakterijske aktivnosti nadkritičnog ekstrakta i etarskog ulja kao i poređenje istih sa literaturnim podacima. Za kvalitativnu i kvantitativnu analizu hemijskog sastava nadkritičnog ekstrakta i etarskog ulja korišćene su GC-FID i GC-MS analitičke metode. Hemijski sastav nadkritičnog ekstrakta i ulja lovora bio je veoma različit. Najzastupljenije komponente u etarskom ulju bili su monoterpeni i njihovi kiseonični derivati (98,4 %), pre svega 1,8-cineol (33,4 %), linalool (16,0 %), α-terpinil-acetat (13,8 %), sabinen (6,91 %) i metil-eugenol (5,32 %). Nadkritični ekstrakt lovora sadržao je dva puta manju količinu monoterpena i njihovih kiseoničnih derivata u odnosu na etarsko ulje (43,89 %) pored seskviterpena (12,43 %), diterpena (1,33 %) i estra (31,13 %). U nadkritičnom ekstraktu najzastupljenije komponente bili su metil-linoleat (16,18 %), α-terpinil-acetat (12,88 %), linalool (9,00 %), metil-eugenol (8,67 %), metil-arahidonat (6,28 %) i eugenol (6,14 %). Antibakterijsko delovanje nadkritičnog ekstrakta i etarskog ulja lovora ispitivano je na sojevima Staphylococcus primenom makrodilucione metode u bujonu. Sojevi Staphylococcus intermedius bili su najosetljiviji na nadkritični ekstrakt i etarsko ulje lovora pri čemu su vrednosti MIC bile 640 μg/ml.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Supercritical CO2 extract and essential oil of bay (Laurus nobilis L.): Chemical composition and antibacterial activity
T1  - Natkritični ekstrakt i etarsko ulje lovora (Laurus nobilis L.) - hemijski sastav i antibakterijska aktivnost
EP  - 404
IS  - 3
SP  - 395
VL  - 75
DO  - 10.2298/JSC090303003I
ER  - 

@article{
author = "Ivanović, Jasna and Mišić, Dušan and Ristić, Mihailo and Pešić, Olivera and Žižović, Irena",
year = "2010",
abstract = "The present study deals with the supercritical carbon dioxide (SC-CO2) extraction and hydrodistillation (HD) of dried bay leaves (Laurus nobilis L.). The chemical composition and antibacterial activity of the SC-CO2 extract and essential oil (EO) from dried leaves of bay were compared to each other and literature data. Qualitative and quantitative analyzes of the SC-CO2 extract and EO were performed using GC-FID and GC-MS analytical methods. A significant difference in the chemical composition of the SC-CO2 extract and EO was observed. The EO comprised high contents of monoterpenes and their oxygenated derivates (98.4 %), principally 1,8-cineole (33.4 %), linalool (16.0 %) and α-terpinyl acetate (13.8 %), sabinene (6.91 %) and methyl eugenol (5.32 %). The SC-CO2 extract comprised twice less monoterpenes and their oxygenated derivates (43.89 %), together with sesquiterpenes (12.43 %), diterpenes (1.33 %) and esters (31.13 %). The major components were methyl linoleate (16.18 %), α-terpinyl acetate (12.88 %), linalool (9.00 %), methyl eugenol (8.67 %), methyl arachidonate (6.28 %) and eugenol (6.14 %). An investigation of the antibacterial activity of bay SC-CO2 extract and EO was completed on different Staphylococcus strains using the broth macrodilution method. Staphylococcus intermedius strains were the most susceptible to both the SC-CO2 extract and EO (MIC = 640 μg/ml)., U radu je ispitana nadkritična ekstrakcija i hidrodestilacija osušenih listova lovora (Laurus nobilis L.). Prikazana je uporedna analiza hemijskog sastava i antibakterijske aktivnosti nadkritičnog ekstrakta i etarskog ulja kao i poređenje istih sa literaturnim podacima. Za kvalitativnu i kvantitativnu analizu hemijskog sastava nadkritičnog ekstrakta i etarskog ulja korišćene su GC-FID i GC-MS analitičke metode. Hemijski sastav nadkritičnog ekstrakta i ulja lovora bio je veoma različit. Najzastupljenije komponente u etarskom ulju bili su monoterpeni i njihovi kiseonični derivati (98,4 %), pre svega 1,8-cineol (33,4 %), linalool (16,0 %), α-terpinil-acetat (13,8 %), sabinen (6,91 %) i metil-eugenol (5,32 %). Nadkritični ekstrakt lovora sadržao je dva puta manju količinu monoterpena i njihovih kiseoničnih derivata u odnosu na etarsko ulje (43,89 %) pored seskviterpena (12,43 %), diterpena (1,33 %) i estra (31,13 %). U nadkritičnom ekstraktu najzastupljenije komponente bili su metil-linoleat (16,18 %), α-terpinil-acetat (12,88 %), linalool (9,00 %), metil-eugenol (8,67 %), metil-arahidonat (6,28 %) i eugenol (6,14 %). Antibakterijsko delovanje nadkritičnog ekstrakta i etarskog ulja lovora ispitivano je na sojevima Staphylococcus primenom makrodilucione metode u bujonu. Sojevi Staphylococcus intermedius bili su najosetljiviji na nadkritični ekstrakt i etarsko ulje lovora pri čemu su vrednosti MIC bile 640 μg/ml.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Supercritical CO2 extract and essential oil of bay (Laurus nobilis L.): Chemical composition and antibacterial activity, Natkritični ekstrakt i etarsko ulje lovora (Laurus nobilis L.) - hemijski sastav i antibakterijska aktivnost",
pages = "404-395",
number = "3",
volume = "75",
doi = "10.2298/JSC090303003I"
}

Ivanović, J., Mišić, D., Ristić, M., Pešić, O.,& Žižović, I.. (2010). Supercritical CO2 extract and essential oil of bay (Laurus nobilis L.): Chemical composition and antibacterial activity. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 75(3), 395-404.
https://doi.org/10.2298/JSC090303003I

Ivanović J, Mišić D, Ristić M, Pešić O, Žižović I. Supercritical CO2 extract and essential oil of bay (Laurus nobilis L.): Chemical composition and antibacterial activity. in Journal of the Serbian Chemical Society. 2010;75(3):395-404.
doi:10.2298/JSC090303003I .

Ivanović, Jasna, Mišić, Dušan, Ristić, Mihailo, Pešić, Olivera, Žižović, Irena, "Supercritical CO2 extract and essential oil of bay (Laurus nobilis L.): Chemical composition and antibacterial activity" in Journal of the Serbian Chemical Society, 75, no. 3 (2010):395-404,
https://doi.org/10.2298/JSC090303003I . .

TY  - JOUR
AU  - Mišić, Dušan
AU  - Žižović, Irena
AU  - Stamenić, Marko
AU  - Ašanin, Ružica
AU  - Ristić, Mihailo S.
AU  - Petrović, Slobodan
AU  - Skala, Dejan
PY  - 2008
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1225
PB  - Elsevier, Amsterdam
T2  - Biochemical Engineering Journal
T1  - Antimicrobial activity of celery fruit isolates and SFE process modeling
EP  - 152
IS  - 2
SP  - 148
VL  - 42
DO  - 10.1016/j.bej.2008.06.008
ER  - 

@article{
author = "Mišić, Dušan and Žižović, Irena and Stamenić, Marko and Ašanin, Ružica and Ristić, Mihailo S. and Petrović, Slobodan and Skala, Dejan",
year = "2008",
publisher = "Elsevier, Amsterdam",
journal = "Biochemical Engineering Journal",
title = "Antimicrobial activity of celery fruit isolates and SFE process modeling",
pages = "152-148",
number = "2",
volume = "42",
doi = "10.1016/j.bej.2008.06.008"
}

Mišić, D., Žižović, I., Stamenić, M., Ašanin, R., Ristić, M. S., Petrović, S.,& Skala, D.. (2008). Antimicrobial activity of celery fruit isolates and SFE process modeling. in Biochemical Engineering Journal
Elsevier, Amsterdam., 42(2), 148-152.
https://doi.org/10.1016/j.bej.2008.06.008

Mišić D, Žižović I, Stamenić M, Ašanin R, Ristić MS, Petrović S, Skala D. Antimicrobial activity of celery fruit isolates and SFE process modeling. in Biochemical Engineering Journal. 2008;42(2):148-152.
doi:10.1016/j.bej.2008.06.008 .

Mišić, Dušan, Žižović, Irena, Stamenić, Marko, Ašanin, Ružica, Ristić, Mihailo S., Petrović, Slobodan, Skala, Dejan, "Antimicrobial activity of celery fruit isolates and SFE process modeling" in Biochemical Engineering Journal, 42, no. 2 (2008):148-152,
https://doi.org/10.1016/j.bej.2008.06.008 . .

TY  - JOUR
AU  - Glišić, Sandra
AU  - Mišić, Dušan
AU  - Stamenić, Marko
AU  - Žižović, Irena
AU  - Asanin, Ružica
AU  - Skala, Dejan
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1167
AB  - Isolation of carrot fruit (Daucus carrota L., cultivar "Chanteney") essential oil by supercritical carbon dioxide was investigated from the pretreatment of herbaceous matrix and extraction conditions to the chemical composition of obtained extract and its antimicrobial activity. The qualitative and quantitative analyses of the supercritical extract, as well as of the essential oil obtained by hydrodistillation, were done by GC/FID and GC/MS methods. Antimicrobial properties of both samples were investigated against ten species of microorganisms. Experimental results showed that the particle size had no influence on the extraction process. The highest yield was obtained at 40 degrees C and 10 MPa. The main component of the supercritical extract, as well as of the essential oil was carotol. The supercritical extract was characterized by the presence of heavier molecular weight compounds, while some lighter compounds, e.g. pinenes, were not detected. The supercritical extract and the essential oil were the most effective against Gram-positive bacteria.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Supercritical carbon dioxide extraction of carrot fruit essential oil: Chemical composition and antimicrobial activity
EP  - 352
IS  - 1
SP  - 346
VL  - 105
DO  - 10.1016/j.foodchem.2006.11.062
ER  - 

@article{
author = "Glišić, Sandra and Mišić, Dušan and Stamenić, Marko and Žižović, Irena and Asanin, Ružica and Skala, Dejan",
year = "2007",
abstract = "Isolation of carrot fruit (Daucus carrota L., cultivar "Chanteney") essential oil by supercritical carbon dioxide was investigated from the pretreatment of herbaceous matrix and extraction conditions to the chemical composition of obtained extract and its antimicrobial activity. The qualitative and quantitative analyses of the supercritical extract, as well as of the essential oil obtained by hydrodistillation, were done by GC/FID and GC/MS methods. Antimicrobial properties of both samples were investigated against ten species of microorganisms. Experimental results showed that the particle size had no influence on the extraction process. The highest yield was obtained at 40 degrees C and 10 MPa. The main component of the supercritical extract, as well as of the essential oil was carotol. The supercritical extract was characterized by the presence of heavier molecular weight compounds, while some lighter compounds, e.g. pinenes, were not detected. The supercritical extract and the essential oil were the most effective against Gram-positive bacteria.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Supercritical carbon dioxide extraction of carrot fruit essential oil: Chemical composition and antimicrobial activity",
pages = "352-346",
number = "1",
volume = "105",
doi = "10.1016/j.foodchem.2006.11.062"
}

Glišić, S., Mišić, D., Stamenić, M., Žižović, I., Asanin, R.,& Skala, D.. (2007). Supercritical carbon dioxide extraction of carrot fruit essential oil: Chemical composition and antimicrobial activity. in Food Chemistry
Elsevier Sci Ltd, Oxford., 105(1), 346-352.
https://doi.org/10.1016/j.foodchem.2006.11.062

Glišić S, Mišić D, Stamenić M, Žižović I, Asanin R, Skala D. Supercritical carbon dioxide extraction of carrot fruit essential oil: Chemical composition and antimicrobial activity. in Food Chemistry. 2007;105(1):346-352.
doi:10.1016/j.foodchem.2006.11.062 .

Glišić, Sandra, Mišić, Dušan, Stamenić, Marko, Žižović, Irena, Asanin, Ružica, Skala, Dejan, "Supercritical carbon dioxide extraction of carrot fruit essential oil: Chemical composition and antimicrobial activity" in Food Chemistry, 105, no. 1 (2007):346-352,
https://doi.org/10.1016/j.foodchem.2006.11.062 . .