TY  - JOUR
AU  - Milovanović, Stoja
AU  - Lukić, Ivana
AU  - Krgović, Nemanja
AU  - Tadić, Vanja
AU  - Radovanović, Željko
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2024-04
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7140
AB  - The study aimed toward the development of biocompatible and biodegradable polymeric films (starch-chitosan and starch-chitosan-cyclodextrin) with biologically valuable green tea leaf extract using an environmentally friendly integrated process of supercritical fluid extraction (SFE) and supercritical solvent impregnation (SSI). The SFE-SSI process parameters were selected through the initial estimation of the SFE parameters effect (temperatures 35 and 70 °C and the addition of co-solvents ethanol, ethanol/water, and water). The present early-stage work showed that an increase in temperature increased extraction yield (from 0.9 to 4.2%) and caffeine content while it decreased polyphenolic and pigment content as well as antioxidant activity and extract loading onto films (from 7.1 to 0.1%). The addition of co-solvents to sc-CO2 had an adverse effect on extract yield, composition, and loading. The highest recorded total phenolic, flavonoid, chlorophyll A, and caffeine content in extracts were 136.5 mg GE/g, 22.7 mg RE/g, 8.1 mg/g, and 460.0 mg/g, respectively.
PB  - Elsevier B.V.
T2  - Journal of Supercritical Fluids
T1  - Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films
SP  - 106163
VL  - 206
DO  - 10.1016/j.supflu.2023.106163
ER  - 

@article{
author = "Milovanović, Stoja and Lukić, Ivana and Krgović, Nemanja and Tadić, Vanja and Radovanović, Željko and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2024-04",
abstract = "The study aimed toward the development of biocompatible and biodegradable polymeric films (starch-chitosan and starch-chitosan-cyclodextrin) with biologically valuable green tea leaf extract using an environmentally friendly integrated process of supercritical fluid extraction (SFE) and supercritical solvent impregnation (SSI). The SFE-SSI process parameters were selected through the initial estimation of the SFE parameters effect (temperatures 35 and 70 °C and the addition of co-solvents ethanol, ethanol/water, and water). The present early-stage work showed that an increase in temperature increased extraction yield (from 0.9 to 4.2%) and caffeine content while it decreased polyphenolic and pigment content as well as antioxidant activity and extract loading onto films (from 7.1 to 0.1%). The addition of co-solvents to sc-CO2 had an adverse effect on extract yield, composition, and loading. The highest recorded total phenolic, flavonoid, chlorophyll A, and caffeine content in extracts were 136.5 mg GE/g, 22.7 mg RE/g, 8.1 mg/g, and 460.0 mg/g, respectively.",
publisher = "Elsevier B.V.",
journal = "Journal of Supercritical Fluids",
title = "Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films",
pages = "106163",
volume = "206",
doi = "10.1016/j.supflu.2023.106163"
}

Milovanović, S., Lukić, I., Krgović, N., Tadić, V., Radovanović, Ž., Tyśkiewicz, K.,& Konkol, M.. (2024-04). Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films. in Journal of Supercritical Fluids
Elsevier B.V.., 206, 106163.
https://doi.org/10.1016/j.supflu.2023.106163

Milovanović S, Lukić I, Krgović N, Tadić V, Radovanović Ž, Tyśkiewicz K, Konkol M. Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films. in Journal of Supercritical Fluids. 2024;206:106163.
doi:10.1016/j.supflu.2023.106163 .

Milovanović, Stoja, Lukić, Ivana, Krgović, Nemanja, Tadić, Vanja, Radovanović, Željko, Tyśkiewicz, Katarzyna, Konkol, Marcin, "Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films" in Journal of Supercritical Fluids, 206 (2024-04):106163,
https://doi.org/10.1016/j.supflu.2023.106163 . .

TY  - JOUR
AU  - Dimitrijević, Snežana
AU  - Milić, Marija
AU  - Tadić, Vanja
AU  - Maksimović, Svetolik
AU  - Filipović, Vladimir
AU  - Dimitrijević-Branković, Suzana
AU  - Miljković, Miona
AU  - Salamon, Ivan
PY  - 2024-02
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7036
AB  - Within this investigation, the effect of conventional and sophisticated extraction method was evaluated on the chemical composition of black cumin seeds (Nigella sativa L.) essential oils and their bioactive characteristics. Soxhlet extraction at 60 °C by hexane (SEH) and petroleum ether (SEP) was adopted as traditionally used technique, while the supercritical extraction with CO2 (SFE), under the pressure of 10 MPa and temperature of 40 °C, was performed as a modern method, expected to overcome the drawbacks of the conventional solvent extraction. The results showed that SFE enabled up to ten times higher extraction yields, compared to essential oils derived after Soxhlet extraction, however, the bioactive compounds content, such as polyphenols, flavonoids and carotenoids, were extracted in higher quantities in the oil from Soxhlet extraction performance. Thereby, the strongest antioxidant agent was found to be the essential oil obtained from SEP, followed by the oil from SFE and oil obtained from SEH, respectively. The GC-MS analysis revealed that the content of thymoquinone was the highest in the essential oil from SEP (69.58%), and the obtained values are among the highest reported within the available literature data. Also, this oil sample contained about eleven times higher thymoquinone quantities than the essential oil obtained by SFE, which was mostly constituted of a methyl linoleate, a fatty acid methyl ester of linoleic acid. In addition, the essential oil from SEP exerted a value added antimicrobial properties, where it was found to be particularly effective in suppression of the Gram-positive bacteria growth.
PB  - Elsevier B.V.
T2  - Sustainable Chemistry and Pharmacy
T1  - Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique
SP  - 101390
VL  - 37
DO  - 10.1016/j.scp.2023.101390
ER  - 

@article{
author = "Dimitrijević, Snežana and Milić, Marija and Tadić, Vanja and Maksimović, Svetolik and Filipović, Vladimir and Dimitrijević-Branković, Suzana and Miljković, Miona and Salamon, Ivan",
year = "2024-02",
abstract = "Within this investigation, the effect of conventional and sophisticated extraction method was evaluated on the chemical composition of black cumin seeds (Nigella sativa L.) essential oils and their bioactive characteristics. Soxhlet extraction at 60 °C by hexane (SEH) and petroleum ether (SEP) was adopted as traditionally used technique, while the supercritical extraction with CO2 (SFE), under the pressure of 10 MPa and temperature of 40 °C, was performed as a modern method, expected to overcome the drawbacks of the conventional solvent extraction. The results showed that SFE enabled up to ten times higher extraction yields, compared to essential oils derived after Soxhlet extraction, however, the bioactive compounds content, such as polyphenols, flavonoids and carotenoids, were extracted in higher quantities in the oil from Soxhlet extraction performance. Thereby, the strongest antioxidant agent was found to be the essential oil obtained from SEP, followed by the oil from SFE and oil obtained from SEH, respectively. The GC-MS analysis revealed that the content of thymoquinone was the highest in the essential oil from SEP (69.58%), and the obtained values are among the highest reported within the available literature data. Also, this oil sample contained about eleven times higher thymoquinone quantities than the essential oil obtained by SFE, which was mostly constituted of a methyl linoleate, a fatty acid methyl ester of linoleic acid. In addition, the essential oil from SEP exerted a value added antimicrobial properties, where it was found to be particularly effective in suppression of the Gram-positive bacteria growth.",
publisher = "Elsevier B.V.",
journal = "Sustainable Chemistry and Pharmacy",
title = "Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique",
pages = "101390",
volume = "37",
doi = "10.1016/j.scp.2023.101390"
}

Dimitrijević, S., Milić, M., Tadić, V., Maksimović, S., Filipović, V., Dimitrijević-Branković, S., Miljković, M.,& Salamon, I.. (2024-02). Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique. in Sustainable Chemistry and Pharmacy
Elsevier B.V.., 37, 101390.
https://doi.org/10.1016/j.scp.2023.101390

Dimitrijević S, Milić M, Tadić V, Maksimović S, Filipović V, Dimitrijević-Branković S, Miljković M, Salamon I. Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique. in Sustainable Chemistry and Pharmacy. 2024;37:101390.
doi:10.1016/j.scp.2023.101390 .

Dimitrijević, Snežana, Milić, Marija, Tadić, Vanja, Maksimović, Svetolik, Filipović, Vladimir, Dimitrijević-Branković, Suzana, Miljković, Miona, Salamon, Ivan, "Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique" in Sustainable Chemistry and Pharmacy, 37 (2024-02):101390,
https://doi.org/10.1016/j.scp.2023.101390 . .

TY  - JOUR
AU  - Krkobabić, Ana
AU  - Radetić, Maja
AU  - Zille, Andrea
AU  - Ribeiro, Ana Isabel
AU  - Tadić, Vanja
AU  - Ilić-Tomić, Tatjana
AU  - Marković, Darka
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7460
AB  - The syntheses of Ag-based nanoparticles (NPs) with the assistance of plant extracts have been shown to be environmentally benign and cost-effective alternatives to conventional chemical syntheses. This study discusses the application of Paliurus spina-christi, Juglans regia, Humulus lupulus, and Sambucus nigra leaf extracts for in situ synthesis of Ag-based NPs on cotton fabric modified with citric acid. The presence of NPs with an average size ranging from 57 to 99 nm on the fiber surface was confirmed by FESEM. XPS analysis indicated that metallic (Ag0) and/or ionic silver (Ag2O and AgO) appeared on the surface of the modified cotton. The chemical composition, size, shape, and amounts of synthesized NPs were strongly dependent on the applied plant extract. All fabricated nanocomposites exhibited excellent antifungal activity against yeast Candida albicans. Antibacterial activity was significantly stronger against Gram-positive bacteria Staphylococcus aureus than Gram-negative bacteria Escherichia coli. In addition, 99% of silver was retained on the samples after 24 h of contact with physiological saline solution, implying a high stability of nanoparticles. Cytotoxic activity towards HaCaT and MRC5 cells was only observed for the sample synthetized in the presence of H. lupulus extract. Excellent antimicrobial activity and non-cytotoxicity make the developed composites efficient candidates for medicinal applications.
PB  - MDPI
T2  - Molecules
T1  - Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies
IS  - 7
SP  - 1447
VL  - 29
DO  - 10.3390/molecules29071447
ER  - 

@article{
author = "Krkobabić, Ana and Radetić, Maja and Zille, Andrea and Ribeiro, Ana Isabel and Tadić, Vanja and Ilić-Tomić, Tatjana and Marković, Darka",
year = "2024",
abstract = "The syntheses of Ag-based nanoparticles (NPs) with the assistance of plant extracts have been shown to be environmentally benign and cost-effective alternatives to conventional chemical syntheses. This study discusses the application of Paliurus spina-christi, Juglans regia, Humulus lupulus, and Sambucus nigra leaf extracts for in situ synthesis of Ag-based NPs on cotton fabric modified with citric acid. The presence of NPs with an average size ranging from 57 to 99 nm on the fiber surface was confirmed by FESEM. XPS analysis indicated that metallic (Ag0) and/or ionic silver (Ag2O and AgO) appeared on the surface of the modified cotton. The chemical composition, size, shape, and amounts of synthesized NPs were strongly dependent on the applied plant extract. All fabricated nanocomposites exhibited excellent antifungal activity against yeast Candida albicans. Antibacterial activity was significantly stronger against Gram-positive bacteria Staphylococcus aureus than Gram-negative bacteria Escherichia coli. In addition, 99% of silver was retained on the samples after 24 h of contact with physiological saline solution, implying a high stability of nanoparticles. Cytotoxic activity towards HaCaT and MRC5 cells was only observed for the sample synthetized in the presence of H. lupulus extract. Excellent antimicrobial activity and non-cytotoxicity make the developed composites efficient candidates for medicinal applications.",
publisher = "MDPI",
journal = "Molecules",
title = "Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies",
number = "7",
pages = "1447",
volume = "29",
doi = "10.3390/molecules29071447"
}

Krkobabić, A., Radetić, M., Zille, A., Ribeiro, A. I., Tadić, V., Ilić-Tomić, T.,& Marković, D.. (2024). Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies. in Molecules
MDPI., 29(7), 1447.
https://doi.org/10.3390/molecules29071447

Krkobabić A, Radetić M, Zille A, Ribeiro AI, Tadić V, Ilić-Tomić T, Marković D. Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies. in Molecules. 2024;29(7):1447.
doi:10.3390/molecules29071447 .

Krkobabić, Ana, Radetić, Maja, Zille, Andrea, Ribeiro, Ana Isabel, Tadić, Vanja, Ilić-Tomić, Tatjana, Marković, Darka, "Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies" in Molecules, 29, no. 7 (2024):1447,
https://doi.org/10.3390/molecules29071447 . .

TY  - CONF
AU  - Lukić, Ivana
AU  - Milovanović, Stoja
AU  - Krgović, Nemanja
AU  - Tadić, Vanja
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6907
AB  - Green tea is considered to be a plant with a variety of health benefits due to its antioxidative, antimicrobial,
anti-inflammatory, and anticancer activity. Besides the most important bioactive constituents, including
polyphenols, especially catechins, and flavonoids, green tea is rich in caffeine, and such complex composition
makes its extract suitable as a component of active food packaging or skin care systems [1,2]. The
technologies commonly proposed to obtain such materials involve some limitations, mainly related to the use
of liquid organic solvents that lead to low penetration of the active compound into the polymeric platform and,
consequently, limited loading efficiencies, non-unique dispersion, and solvent residue in the final material. An
excellent technique for direct loading of natural bioactive compounds from plant materials into polymeric
carriers that can overcome the aforementioned challenges is an integrated process accomplished by merging
supercritical fluid extraction (SFE) and supercritical solvent impregnation (SSI). Therefore, in this study, the
preparation of two types of polymer films and their functionalization with green tea leaf extract using the SFESSI
process was tested.
Starch/chitosan (SC) films were prepared by mixing the 1% chitosan solution with the 1% starch solution in a
mass ratio of 1:1, while starch/chitosan/β-cyclodextrin (SCCD) films were prepared by addition of 1% β-
cyclodextrin to starch and chitosan to obtain the ratio of 1:1:1. After solvent casting and drying at room
temperature, the films were additionally dried in an oven overnight at 35 °C. Initially, supercritical CO2
(scCO2) extraction was investigated as a method for isolating the extract from green tea at temperatures of 35
and 70 °C and pressure of 30 MPa, with and without the ethanol as a co-solvent. The obtained extraction yields
ranged from 0.91 to 4.22%, being the highest at a higher temperature. The chemical composition of the
obtained extracts was determined, as well as the total phenolic content (TPC), total flavonoid content (TFC),
total chlorophyll, and total carotenoid content. HPLC analysis revealed that caffeine was the major constituent
of all extracts with the amount ranging from 24.1 to 202.9 mg/g. The IC50 values determined using the DPPH
assay showed that the extract obtained at 35 °C with the addition of ethanol exhibited the strongest antioxidant
activity. After determining the SFE conditions that gave the green tea extract with high content of bioactive
compounds, prepared films were impregnated using the integrated SFE-SSI process for 4 h at the same
conditions as scCO2 extraction. The loading of green tea extracts onto films was up to 7.7% showing that lower
temperature (35 °C) and the addition of ethanol had a favourable influence. The presence of the extract on the
surface of impregnated samples was confirmed by FTIR analysis.
PB  - Budapest : University of Technology and Economics
C3  - Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
T1  - Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films
SP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6907
ER  - 

@conference{
author = "Lukić, Ivana and Milovanović, Stoja and Krgović, Nemanja and Tadić, Vanja and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2023",
abstract = "Green tea is considered to be a plant with a variety of health benefits due to its antioxidative, antimicrobial,
anti-inflammatory, and anticancer activity. Besides the most important bioactive constituents, including
polyphenols, especially catechins, and flavonoids, green tea is rich in caffeine, and such complex composition
makes its extract suitable as a component of active food packaging or skin care systems [1,2]. The
technologies commonly proposed to obtain such materials involve some limitations, mainly related to the use
of liquid organic solvents that lead to low penetration of the active compound into the polymeric platform and,
consequently, limited loading efficiencies, non-unique dispersion, and solvent residue in the final material. An
excellent technique for direct loading of natural bioactive compounds from plant materials into polymeric
carriers that can overcome the aforementioned challenges is an integrated process accomplished by merging
supercritical fluid extraction (SFE) and supercritical solvent impregnation (SSI). Therefore, in this study, the
preparation of two types of polymer films and their functionalization with green tea leaf extract using the SFESSI
process was tested.
Starch/chitosan (SC) films were prepared by mixing the 1% chitosan solution with the 1% starch solution in a
mass ratio of 1:1, while starch/chitosan/β-cyclodextrin (SCCD) films were prepared by addition of 1% β-
cyclodextrin to starch and chitosan to obtain the ratio of 1:1:1. After solvent casting and drying at room
temperature, the films were additionally dried in an oven overnight at 35 °C. Initially, supercritical CO2
(scCO2) extraction was investigated as a method for isolating the extract from green tea at temperatures of 35
and 70 °C and pressure of 30 MPa, with and without the ethanol as a co-solvent. The obtained extraction yields
ranged from 0.91 to 4.22%, being the highest at a higher temperature. The chemical composition of the
obtained extracts was determined, as well as the total phenolic content (TPC), total flavonoid content (TFC),
total chlorophyll, and total carotenoid content. HPLC analysis revealed that caffeine was the major constituent
of all extracts with the amount ranging from 24.1 to 202.9 mg/g. The IC50 values determined using the DPPH
assay showed that the extract obtained at 35 °C with the addition of ethanol exhibited the strongest antioxidant
activity. After determining the SFE conditions that gave the green tea extract with high content of bioactive
compounds, prepared films were impregnated using the integrated SFE-SSI process for 4 h at the same
conditions as scCO2 extraction. The loading of green tea extracts onto films was up to 7.7% showing that lower
temperature (35 °C) and the addition of ethanol had a favourable influence. The presence of the extract on the
surface of impregnated samples was confirmed by FTIR analysis.",
publisher = "Budapest : University of Technology and Economics",
journal = "Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023",
title = "Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films",
pages = "124",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6907"
}

Lukić, I., Milovanović, S., Krgović, N., Tadić, V., Tyśkiewicz, K.,& Konkol, M.. (2023). Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
Budapest : University of Technology and Economics., 124.
https://hdl.handle.net/21.15107/rcub_technorep_6907

Lukić I, Milovanović S, Krgović N, Tadić V, Tyśkiewicz K, Konkol M. Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023. 2023;:124.
https://hdl.handle.net/21.15107/rcub_technorep_6907 .

Lukić, Ivana, Milovanović, Stoja, Krgović, Nemanja, Tadić, Vanja, Tyśkiewicz, Katarzyna, Konkol, Marcin, "Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films" in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 (2023):124,
https://hdl.handle.net/21.15107/rcub_technorep_6907 .

TY  - JOUR
AU  - Maksimović, Svetolik
AU  - Stanković, Milica
AU  - Roganović, Sonja
AU  - Nešić, Ivana
AU  - Zvezdanović, Jelena
AU  - Tadić, Vanja
AU  - Žižović, Irena
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5230
AB  - Ethnopharmacological relevance: Helichrysum italicum has been widely used in traditional medicine to treat allergies, colds, cough, skin, liver and gallbladder disorders, inflammation, infections, and sleeplessness. Furthermore, it possesses considerable wound healing and skin protective properties, documented by several in vivo studies performed on animals. However, there is a lack of experimental evidence supporting its potential as a topical agent tested by human clinical trials. Aim of the study: The study aimed to investigate the skin protective activity of cotton gauze and polypropylene non-woven fabric, impregnated with H. italicum extract by the integrated supercritical CO2 extraction-supercritical solvent impregnation process. Materials and methods: The integrated process of supercritical CO2 extraction of H. italicum and the impregnation of cotton gauze and polypropylene non-woven fabric was performed under 350 bar and 40 °C with and without the addition of ethanol as a cosolvent. Impregnated textile materials were tested in vivo for their bioactivity on irritated human skin. Randomized in vivo studies performed involved assays of both safety and efficacy of the impregnated textiles. The effects were evaluated using the in vivo non-invasive biophysical measurements of the following skin parameters: electrical capacitance, transepidermal water loss, melanin index, erythema index, and skin pH. Results: Both cotton gauze and polypropylene non-woven fabric were impregnated with H. italicum extracts under supercritical conditions with considerable values of the impregnation yield (1.97%–4.25%). The addition of ethanol as a cosolvent during the process caused significant changes in the incorporated extracts’ impregnation yield and chemical profile. Both impregnated textile materials were safe, evaluated by their testing on the human skin with no cause of any irritation and redness. However, efficacy studies revealed that polypropylene non-woven fabric impregnated with H. italicum extract with ethanol as a cosolvent, possessed significantly greater potential for skin protection than the other investigated samples. Conclusions: The present study demonstrated the feasibility of the combined supercritical extraction and impregnation process in developing materials for topical application based on H. italicum extract. The results of in vivo studies performed on human volunteers confirmed the suitability of H. italicum active components to be a part of human skin protective preparations because of their ability to maintain the skin unimpaired. Traditionally claimed applications as a medicinal plant capable of regenerating skin have been scientifically proven, in addition to employing green technology in obtaining the impregnated materials with a broad spectrum of utilization.
PB  - Elsevier Ireland Ltd
T2  - Journal of Ethnopharmacology
T1  - Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin
SP  - 115779
VL  - 301
DO  - 10.1016/j.jep.2022.115779
ER  - 

@article{
author = "Maksimović, Svetolik and Stanković, Milica and Roganović, Sonja and Nešić, Ivana and Zvezdanović, Jelena and Tadić, Vanja and Žižović, Irena",
year = "2023",
abstract = "Ethnopharmacological relevance: Helichrysum italicum has been widely used in traditional medicine to treat allergies, colds, cough, skin, liver and gallbladder disorders, inflammation, infections, and sleeplessness. Furthermore, it possesses considerable wound healing and skin protective properties, documented by several in vivo studies performed on animals. However, there is a lack of experimental evidence supporting its potential as a topical agent tested by human clinical trials. Aim of the study: The study aimed to investigate the skin protective activity of cotton gauze and polypropylene non-woven fabric, impregnated with H. italicum extract by the integrated supercritical CO2 extraction-supercritical solvent impregnation process. Materials and methods: The integrated process of supercritical CO2 extraction of H. italicum and the impregnation of cotton gauze and polypropylene non-woven fabric was performed under 350 bar and 40 °C with and without the addition of ethanol as a cosolvent. Impregnated textile materials were tested in vivo for their bioactivity on irritated human skin. Randomized in vivo studies performed involved assays of both safety and efficacy of the impregnated textiles. The effects were evaluated using the in vivo non-invasive biophysical measurements of the following skin parameters: electrical capacitance, transepidermal water loss, melanin index, erythema index, and skin pH. Results: Both cotton gauze and polypropylene non-woven fabric were impregnated with H. italicum extracts under supercritical conditions with considerable values of the impregnation yield (1.97%–4.25%). The addition of ethanol as a cosolvent during the process caused significant changes in the incorporated extracts’ impregnation yield and chemical profile. Both impregnated textile materials were safe, evaluated by their testing on the human skin with no cause of any irritation and redness. However, efficacy studies revealed that polypropylene non-woven fabric impregnated with H. italicum extract with ethanol as a cosolvent, possessed significantly greater potential for skin protection than the other investigated samples. Conclusions: The present study demonstrated the feasibility of the combined supercritical extraction and impregnation process in developing materials for topical application based on H. italicum extract. The results of in vivo studies performed on human volunteers confirmed the suitability of H. italicum active components to be a part of human skin protective preparations because of their ability to maintain the skin unimpaired. Traditionally claimed applications as a medicinal plant capable of regenerating skin have been scientifically proven, in addition to employing green technology in obtaining the impregnated materials with a broad spectrum of utilization.",
publisher = "Elsevier Ireland Ltd",
journal = "Journal of Ethnopharmacology",
title = "Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin",
pages = "115779",
volume = "301",
doi = "10.1016/j.jep.2022.115779"
}

Maksimović, S., Stanković, M., Roganović, S., Nešić, I., Zvezdanović, J., Tadić, V.,& Žižović, I.. (2023). Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin. in Journal of Ethnopharmacology
Elsevier Ireland Ltd., 301, 115779.
https://doi.org/10.1016/j.jep.2022.115779

Maksimović S, Stanković M, Roganović S, Nešić I, Zvezdanović J, Tadić V, Žižović I. Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin. in Journal of Ethnopharmacology. 2023;301:115779.
doi:10.1016/j.jep.2022.115779 .

Maksimović, Svetolik, Stanković, Milica, Roganović, Sonja, Nešić, Ivana, Zvezdanović, Jelena, Tadić, Vanja, Žižović, Irena, "Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin" in Journal of Ethnopharmacology, 301 (2023):115779,
https://doi.org/10.1016/j.jep.2022.115779 . .

TY  - CONF
AU  - Lukić, Ivana
AU  - Stamenić, Marko
AU  - Tadić, Vanja
AU  - Skala, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6984
AB  - The high potential of industrial hemp (Cannabis sativa), mainly due to the high-quality fibers and bioactive compounds, coupled with the decriminalization and/or legalization in some regions of the world, led to the steady growth of scientific research over the past couple of decades [1, 2]. From an industrial point of view, cannabinoids, primarily non-psychotropic cannabidiol (CBD), are considered the most valuable compounds, as they possess a broad range of therapeutic properties, such as anxiolytic, neuroprotective, antibiotic, antiinflammatory activity, and anti-oxidant. The hemp industry has already adopted supercritical carbon dioxide scCO2 extraction as a well-established process since it is a promising ‘green’ alternative to conventional extraction methods, selective, with short processing time, and low impact on the environment.
In the present study, the preliminary analysis was performed to determine the influence of different
scCO2 densities based on the different combinations of process pressure and temperature on the kinetics of supercritical extraction of Cannabis sativa, extract yield and the chemical composition of obtained extracts. Experiments were performed at temperatures ranging from 40 to 60 °C and pressures ranging from 10 to 30 MPa. Prior to extraction, the plant material was grounded and submitted to decarboxylation process at 120 °C for 1 h, in order to transform cannabidiolic acid, which is the precursor present in the plant material, to CBD.
Experimental design for optimization plays an important role both in science and industry, because it allows evaluation of the effects of multiple factors and their interactions on one or more response variables. The present work was focused on the further optimization of scCO2 extraction with the aim to maximize the yield of extracts with high recoveries of CBD, by means of response surface methodology (RSM), based on a central composite design (CCD) using Design of Experiment software (Stat-Ease, Design Expert). For this purpose, independent variables were temperature (40-60 °C), pressure (10-30 MPa), and scCO2 consumption (20-60 gCO2/gplant).
The results showed that supercritical CO2 extraction enabled the achievement of up to 5% of total extract yield and 266–435 mg/g of CBD depending on the conditions. The high pressure and temperature, 30 MPa 60 °C, respectively, conditions that correspond to the highest density of the scCO2, generated the highest extract yield. On the contrary, lower pressure, corresponding to the lower density of CO2 was favourable for achieving a high CBD content. In addition, a higher scCO2 consumption, which is related to longer processing time, was the most dominant factor affecting the extraction process exerting a significant effect, on both examined responses, yield and CBD content. A linear model equation was proposed to express both the yield and the CBD content as a function of independent variables.
PB  - Budapest : University of Technology and Economics
C3  - Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
T1  - Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach
SP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6984
ER  - 

@conference{
author = "Lukić, Ivana and Stamenić, Marko and Tadić, Vanja and Skala, Dejan",
year = "2023",
abstract = "The high potential of industrial hemp (Cannabis sativa), mainly due to the high-quality fibers and bioactive compounds, coupled with the decriminalization and/or legalization in some regions of the world, led to the steady growth of scientific research over the past couple of decades [1, 2]. From an industrial point of view, cannabinoids, primarily non-psychotropic cannabidiol (CBD), are considered the most valuable compounds, as they possess a broad range of therapeutic properties, such as anxiolytic, neuroprotective, antibiotic, antiinflammatory activity, and anti-oxidant. The hemp industry has already adopted supercritical carbon dioxide scCO2 extraction as a well-established process since it is a promising ‘green’ alternative to conventional extraction methods, selective, with short processing time, and low impact on the environment.
In the present study, the preliminary analysis was performed to determine the influence of different
scCO2 densities based on the different combinations of process pressure and temperature on the kinetics of supercritical extraction of Cannabis sativa, extract yield and the chemical composition of obtained extracts. Experiments were performed at temperatures ranging from 40 to 60 °C and pressures ranging from 10 to 30 MPa. Prior to extraction, the plant material was grounded and submitted to decarboxylation process at 120 °C for 1 h, in order to transform cannabidiolic acid, which is the precursor present in the plant material, to CBD.
Experimental design for optimization plays an important role both in science and industry, because it allows evaluation of the effects of multiple factors and their interactions on one or more response variables. The present work was focused on the further optimization of scCO2 extraction with the aim to maximize the yield of extracts with high recoveries of CBD, by means of response surface methodology (RSM), based on a central composite design (CCD) using Design of Experiment software (Stat-Ease, Design Expert). For this purpose, independent variables were temperature (40-60 °C), pressure (10-30 MPa), and scCO2 consumption (20-60 gCO2/gplant).
The results showed that supercritical CO2 extraction enabled the achievement of up to 5% of total extract yield and 266–435 mg/g of CBD depending on the conditions. The high pressure and temperature, 30 MPa 60 °C, respectively, conditions that correspond to the highest density of the scCO2, generated the highest extract yield. On the contrary, lower pressure, corresponding to the lower density of CO2 was favourable for achieving a high CBD content. In addition, a higher scCO2 consumption, which is related to longer processing time, was the most dominant factor affecting the extraction process exerting a significant effect, on both examined responses, yield and CBD content. A linear model equation was proposed to express both the yield and the CBD content as a function of independent variables.",
publisher = "Budapest : University of Technology and Economics",
journal = "Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023",
title = "Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach",
pages = "135",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6984"
}

Lukić, I., Stamenić, M., Tadić, V.,& Skala, D.. (2023). Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
Budapest : University of Technology and Economics., 135.
https://hdl.handle.net/21.15107/rcub_technorep_6984

Lukić I, Stamenić M, Tadić V, Skala D. Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023. 2023;:135.
https://hdl.handle.net/21.15107/rcub_technorep_6984 .

Lukić, Ivana, Stamenić, Marko, Tadić, Vanja, Skala, Dejan, "Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach" in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 (2023):135,
https://hdl.handle.net/21.15107/rcub_technorep_6984 .

TY  - PAT
AU  - Tadić, Vanja
AU  - Žugić, Ana
AU  - Nešić, Ivana
AU  - Krgović, Nemanja
AU  - Mudrić, Jelena
AU  - Martinović, Milica
AU  - Stojanović, Sanja
AU  - Maksimović, Svetolik
AU  - Lukić, Ivana
AU  - Petrović, Slobodan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6924
T2  - Zavod za intelektualnu svojinu republike Srbije
T1  - Gastroretentivni fitopreparat sa sistemskim delovanjem za sniženje nivoa šećera u krvi i postupak dobijanja
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6924
ER  - 

@misc{
author = "Tadić, Vanja and Žugić, Ana and Nešić, Ivana and Krgović, Nemanja and Mudrić, Jelena and Martinović, Milica and Stojanović, Sanja and Maksimović, Svetolik and Lukić, Ivana and Petrović, Slobodan",
year = "2023",
journal = "Zavod za intelektualnu svojinu republike Srbije",
title = "Gastroretentivni fitopreparat sa sistemskim delovanjem za sniženje nivoa šećera u krvi i postupak dobijanja",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6924"
}

Tadić, V., Žugić, A., Nešić, I., Krgović, N., Mudrić, J., Martinović, M., Stojanović, S., Maksimović, S., Lukić, I.,& Petrović, S.. (2023). Gastroretentivni fitopreparat sa sistemskim delovanjem za sniženje nivoa šećera u krvi i postupak dobijanja. in Zavod za intelektualnu svojinu republike Srbije.
https://hdl.handle.net/21.15107/rcub_technorep_6924

Tadić V, Žugić A, Nešić I, Krgović N, Mudrić J, Martinović M, Stojanović S, Maksimović S, Lukić I, Petrović S. Gastroretentivni fitopreparat sa sistemskim delovanjem za sniženje nivoa šećera u krvi i postupak dobijanja. in Zavod za intelektualnu svojinu republike Srbije. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6924 .

Tadić, Vanja, Žugić, Ana, Nešić, Ivana, Krgović, Nemanja, Mudrić, Jelena, Martinović, Milica, Stojanović, Sanja, Maksimović, Svetolik, Lukić, Ivana, Petrović, Slobodan, "Gastroretentivni fitopreparat sa sistemskim delovanjem za sniženje nivoa šećera u krvi i postupak dobijanja" in Zavod za intelektualnu svojinu republike Srbije (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6924 .

TY  - CONF
AU  - Lukić, Ivana
AU  - Milovanović, Stoja
AU  - Tadić, Vanja
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6791
AB  - Bilberries (Vaccinium myrtillus) have been used in traditional medicine to reduce
inflammation and protect against diseases associated with oxidative stress, such as
cardiovascular disease, diabetes, and age-related cognitive decline, as well as a
remedy for eye conditions. The biological activity of bilberries is ascribed mainly to
their high content of anthocyanins, but also to other valuable phytochemicals,
including flavonols, phenolic acids, and tannins [1,2]. The enhanced stability and
bioavailability of bioactive components from plant material could be accomplished
by their incorporation into a polymeric carrier, using the promising integrated
processes of supercritical fluid extraction (SFE) and supercritical solvent
impregnation (SSI). ...
PB  - Maribor : University of Maribor
PB  - Maribor : University Press
C3  - Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia
T1  - Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel
EP  - 109
SP  - 107
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6791
ER  - 

@conference{
author = "Lukić, Ivana and Milovanović, Stoja and Tadić, Vanja",
year = "2023",
abstract = "Bilberries (Vaccinium myrtillus) have been used in traditional medicine to reduce
inflammation and protect against diseases associated with oxidative stress, such as
cardiovascular disease, diabetes, and age-related cognitive decline, as well as a
remedy for eye conditions. The biological activity of bilberries is ascribed mainly to
their high content of anthocyanins, but also to other valuable phytochemicals,
including flavonols, phenolic acids, and tannins [1,2]. The enhanced stability and
bioavailability of bioactive components from plant material could be accomplished
by their incorporation into a polymeric carrier, using the promising integrated
processes of supercritical fluid extraction (SFE) and supercritical solvent
impregnation (SSI). ...",
publisher = "Maribor : University of Maribor, Maribor : University Press",
journal = "Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia",
title = "Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel",
pages = "109-107",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6791"
}

Lukić, I., Milovanović, S.,& Tadić, V.. (2023). Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel. in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia
Maribor : University of Maribor., 107-109.
https://hdl.handle.net/21.15107/rcub_technorep_6791

Lukić I, Milovanović S, Tadić V. Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel. in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia. 2023;:107-109.
https://hdl.handle.net/21.15107/rcub_technorep_6791 .

Lukić, Ivana, Milovanović, Stoja, Tadić, Vanja, "Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel" in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia (2023):107-109,
https://hdl.handle.net/21.15107/rcub_technorep_6791 .

TY  - PAT
AU  - Tadić, Vanja
AU  - Nešić, Ivana
AU  - Milenković, Marina
AU  - Martinović, Milica
AU  - Žugić, Ana
AU  - Maksimović, Svetolik
AU  - Stojanović, Sanja
AU  - Milovanović, Stoja
AU  - Krgović, Nemanja
AU  - Petrović, Slobodan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6900
T2  - Zavod za intelektualnu svojinu republike Srbije
T1  - Liposomski preparati za topikalnu primenu kod osoba sa hiperglikemijom
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6900
ER  - 

@misc{
author = "Tadić, Vanja and Nešić, Ivana and Milenković, Marina and Martinović, Milica and Žugić, Ana and Maksimović, Svetolik and Stojanović, Sanja and Milovanović, Stoja and Krgović, Nemanja and Petrović, Slobodan",
year = "2023",
journal = "Zavod za intelektualnu svojinu republike Srbije",
title = "Liposomski preparati za topikalnu primenu kod osoba sa hiperglikemijom",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6900"
}

Tadić, V., Nešić, I., Milenković, M., Martinović, M., Žugić, A., Maksimović, S., Stojanović, S., Milovanović, S., Krgović, N.,& Petrović, S.. (2023). Liposomski preparati za topikalnu primenu kod osoba sa hiperglikemijom. in Zavod za intelektualnu svojinu republike Srbije.
https://hdl.handle.net/21.15107/rcub_technorep_6900

Tadić V, Nešić I, Milenković M, Martinović M, Žugić A, Maksimović S, Stojanović S, Milovanović S, Krgović N, Petrović S. Liposomski preparati za topikalnu primenu kod osoba sa hiperglikemijom. in Zavod za intelektualnu svojinu republike Srbije. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6900 .

Tadić, Vanja, Nešić, Ivana, Milenković, Marina, Martinović, Milica, Žugić, Ana, Maksimović, Svetolik, Stojanović, Sanja, Milovanović, Stoja, Krgović, Nemanja, Petrović, Slobodan, "Liposomski preparati za topikalnu primenu kod osoba sa hiperglikemijom" in Zavod za intelektualnu svojinu republike Srbije (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6900 .

TY  - JOUR
AU  - Vukmirović, Saša
AU  - Ilić, Vladimirka
AU  - Tadić, Vanja
AU  - Čapo, Ivan
AU  - Pavlović, Nebojša
AU  - Tomas, Ana
AU  - Paut Kusturica, Milica
AU  - Tomić, Nataša
AU  - Maksimović, Svetolik
AU  - Stilinović, Nebojša
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6221
AB  - The framework of this study was a comprehensive investigation of Morus nigra L. extracts, with the aim to establish the correlation between chemical composition and antioxidant/hepatoprotective activity of a series of black mulberry extracts obtained from aerial parts of the plant. Black mulberry leaf (MLEE), bark (MBEE), juice (MJ) and fresh fruit (MFEE) extracts were obtained using the conventional Soxhlet extraction, while the supercritical CO2 extraction procedure was employed for preparation of the seed oil (MSO). Analysis of the chemical composition was performed using spectrophotometric, HPLC and GC methods. For the evaluation of antioxidant activity, in vitro FRAP and DPPH assays were applied. In Haan strain NMRI mice with streptozotocin-induced oxidative stress, in vivo antioxidant activity and liver tissue integrity were examined. The content of polyphenolic compounds was the highest in MBEE (68.3 ± 0.7 mgGAE/g) with the most abundant compounds being polyphenolic acids, followed by MLEE (23.4 ± 0.5 mgGAE/g) with the flavonoids isoquercetin and rutin being present in a significant amount. An analysis of MSO revealed a high content of γ-linoleic acid. The highest antioxidant activity in vitro (FRAP and DPPH) was observed for MLEE, MBEE and MSO. Beneficial effects were confirmed in vivo, with lower values of hepatosomatic index, potentiation of the activity of the enzymes superoxide dismutase and catalase, a lower rate of lipid peroxidation and reduced positivity for the P450 enzyme in animals treated with MLEE, MBEE and MSO. Black mulberry leaf and bark extracts as well as seed oil exhibited significant antioxidant activity. Apart from the confirmed biological properties of the fruit and leaf extracts, the observed activities of black mulberry seed oil and bark extract imply its importance as a sustainable source of phytochemicals.
PB  - MDPI
T2  - Antioxidants
T1  - Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L.
IS  - 2
SP  - 382
VL  - 12
DO  - 10.3390/antiox12020382
ER  - 

@article{
author = "Vukmirović, Saša and Ilić, Vladimirka and Tadić, Vanja and Čapo, Ivan and Pavlović, Nebojša and Tomas, Ana and Paut Kusturica, Milica and Tomić, Nataša and Maksimović, Svetolik and Stilinović, Nebojša",
year = "2023",
abstract = "The framework of this study was a comprehensive investigation of Morus nigra L. extracts, with the aim to establish the correlation between chemical composition and antioxidant/hepatoprotective activity of a series of black mulberry extracts obtained from aerial parts of the plant. Black mulberry leaf (MLEE), bark (MBEE), juice (MJ) and fresh fruit (MFEE) extracts were obtained using the conventional Soxhlet extraction, while the supercritical CO2 extraction procedure was employed for preparation of the seed oil (MSO). Analysis of the chemical composition was performed using spectrophotometric, HPLC and GC methods. For the evaluation of antioxidant activity, in vitro FRAP and DPPH assays were applied. In Haan strain NMRI mice with streptozotocin-induced oxidative stress, in vivo antioxidant activity and liver tissue integrity were examined. The content of polyphenolic compounds was the highest in MBEE (68.3 ± 0.7 mgGAE/g) with the most abundant compounds being polyphenolic acids, followed by MLEE (23.4 ± 0.5 mgGAE/g) with the flavonoids isoquercetin and rutin being present in a significant amount. An analysis of MSO revealed a high content of γ-linoleic acid. The highest antioxidant activity in vitro (FRAP and DPPH) was observed for MLEE, MBEE and MSO. Beneficial effects were confirmed in vivo, with lower values of hepatosomatic index, potentiation of the activity of the enzymes superoxide dismutase and catalase, a lower rate of lipid peroxidation and reduced positivity for the P450 enzyme in animals treated with MLEE, MBEE and MSO. Black mulberry leaf and bark extracts as well as seed oil exhibited significant antioxidant activity. Apart from the confirmed biological properties of the fruit and leaf extracts, the observed activities of black mulberry seed oil and bark extract imply its importance as a sustainable source of phytochemicals.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L.",
number = "2",
pages = "382",
volume = "12",
doi = "10.3390/antiox12020382"
}

Vukmirović, S., Ilić, V., Tadić, V., Čapo, I., Pavlović, N., Tomas, A., Paut Kusturica, M., Tomić, N., Maksimović, S.,& Stilinović, N.. (2023). Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L.. in Antioxidants
MDPI., 12(2), 382.
https://doi.org/10.3390/antiox12020382

Vukmirović S, Ilić V, Tadić V, Čapo I, Pavlović N, Tomas A, Paut Kusturica M, Tomić N, Maksimović S, Stilinović N. Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L.. in Antioxidants. 2023;12(2):382.
doi:10.3390/antiox12020382 .

Vukmirović, Saša, Ilić, Vladimirka, Tadić, Vanja, Čapo, Ivan, Pavlović, Nebojša, Tomas, Ana, Paut Kusturica, Milica, Tomić, Nataša, Maksimović, Svetolik, Stilinović, Nebojša, "Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L." in Antioxidants, 12, no. 2 (2023):382,
https://doi.org/10.3390/antiox12020382 . .

TY  - CONF
AU  - Krkobabić, Ana G.
AU  - Marković, Darka D.
AU  - Kovačević, Aleksandar G.
AU  - Ilić-Tomić, Tatjana R.
AU  - Tadić, Vanja M.
AU  - Radetić, Maja M.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6881
AB  - U ovom radu je ispitana mogućnost in situ sinteze nanočestica na bazi srebra primenom ekstrakta lista oraha, na pamučnoj tkanini prethodno modifikovanoj limunskom kiselinom. Tokom sinteze su formirane sferne nanočestice prečnika oko 60 nm, koje su ravnomerno raspoređene po površini pamučnih vlakana. Prisustvo nanočestica na bazi srebra je obezbedilo odličnu antimikrobnu aktivnost prema bakterijama Staphylococcus aureus i Escherichia coli, kao i kvascu Candida albicans. Tekstilni nanokompozitni materijal nije citotoksičan prema ćelijama zdravih keratinocita kože (HaCaT linija) i zdravih fibroblasta (MRC-5 linija), što omogućava njegovu bezbednu primenu za medicinske potrebe.
AB  - This study discusses the possibility to utilize walnut leaf extract for in situ synthesis of
silver-based nanoparticles on cotton fabric previously modified with citric acid.
Synthetized spherical nanoparticles with an average diameter of 60 nm were evenly
distributed over the surface of cotton fibers. The presence of silver-based nanoparticles
provided excellent antimicrobial activity against bacteria Staphylococcus aureus and
Escherichia coli, and yeast Candida albicans. The textile nanocomposite did not show any
cytotoxicity towards healthy skin keratinocytes cells (HaCaT line) and healthy fibroblast
cells (MRC-5 line). Thus, it could be considered as a safe for potential medical
applications.
PB  - Beograd : Srpsko hemijsko društvo
C3  - Kratki izvodi radova ; Knjiga radova / 59. savetovanje Srpskog hemijskog društva, Novi Sad 1. i 2. jun 2023. godine
T1  - Antimikrobna aktivnost i citotoksičnost nanočestica na bazi srebra in situ sintetisanih na pamučnoj tkanini primenom ekstrakta lista oraha
T1  - Antimicrobial activity and cytotoxicity of silver-based nanoparticles in situ synthesized on cotton fabric using walnut leaf
SP  - 150
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6881
ER  - 

@conference{
author = "Krkobabić, Ana G. and Marković, Darka D. and Kovačević, Aleksandar G. and Ilić-Tomić, Tatjana R. and Tadić, Vanja M. and Radetić, Maja M.",
year = "2023",
abstract = "U ovom radu je ispitana mogućnost in situ sinteze nanočestica na bazi srebra primenom ekstrakta lista oraha, na pamučnoj tkanini prethodno modifikovanoj limunskom kiselinom. Tokom sinteze su formirane sferne nanočestice prečnika oko 60 nm, koje su ravnomerno raspoređene po površini pamučnih vlakana. Prisustvo nanočestica na bazi srebra je obezbedilo odličnu antimikrobnu aktivnost prema bakterijama Staphylococcus aureus i Escherichia coli, kao i kvascu Candida albicans. Tekstilni nanokompozitni materijal nije citotoksičan prema ćelijama zdravih keratinocita kože (HaCaT linija) i zdravih fibroblasta (MRC-5 linija), što omogućava njegovu bezbednu primenu za medicinske potrebe., This study discusses the possibility to utilize walnut leaf extract for in situ synthesis of
silver-based nanoparticles on cotton fabric previously modified with citric acid.
Synthetized spherical nanoparticles with an average diameter of 60 nm were evenly
distributed over the surface of cotton fibers. The presence of silver-based nanoparticles
provided excellent antimicrobial activity against bacteria Staphylococcus aureus and
Escherichia coli, and yeast Candida albicans. The textile nanocomposite did not show any
cytotoxicity towards healthy skin keratinocytes cells (HaCaT line) and healthy fibroblast
cells (MRC-5 line). Thus, it could be considered as a safe for potential medical
applications.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "Kratki izvodi radova ; Knjiga radova / 59. savetovanje Srpskog hemijskog društva, Novi Sad 1. i 2. jun 2023. godine",
title = "Antimikrobna aktivnost i citotoksičnost nanočestica na bazi srebra in situ sintetisanih na pamučnoj tkanini primenom ekstrakta lista oraha, Antimicrobial activity and cytotoxicity of silver-based nanoparticles in situ synthesized on cotton fabric using walnut leaf",
pages = "150",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6881"
}

Krkobabić, A. G., Marković, D. D., Kovačević, A. G., Ilić-Tomić, T. R., Tadić, V. M.,& Radetić, M. M.. (2023). Antimikrobna aktivnost i citotoksičnost nanočestica na bazi srebra in situ sintetisanih na pamučnoj tkanini primenom ekstrakta lista oraha. in Kratki izvodi radova ; Knjiga radova / 59. savetovanje Srpskog hemijskog društva, Novi Sad 1. i 2. jun 2023. godine
Beograd : Srpsko hemijsko društvo., 150.
https://hdl.handle.net/21.15107/rcub_technorep_6881

Krkobabić AG, Marković DD, Kovačević AG, Ilić-Tomić TR, Tadić VM, Radetić MM. Antimikrobna aktivnost i citotoksičnost nanočestica na bazi srebra in situ sintetisanih na pamučnoj tkanini primenom ekstrakta lista oraha. in Kratki izvodi radova ; Knjiga radova / 59. savetovanje Srpskog hemijskog društva, Novi Sad 1. i 2. jun 2023. godine. 2023;:150.
https://hdl.handle.net/21.15107/rcub_technorep_6881 .

Krkobabić, Ana G., Marković, Darka D., Kovačević, Aleksandar G., Ilić-Tomić, Tatjana R., Tadić, Vanja M., Radetić, Maja M., "Antimikrobna aktivnost i citotoksičnost nanočestica na bazi srebra in situ sintetisanih na pamučnoj tkanini primenom ekstrakta lista oraha" in Kratki izvodi radova ; Knjiga radova / 59. savetovanje Srpskog hemijskog društva, Novi Sad 1. i 2. jun 2023. godine (2023):150,
https://hdl.handle.net/21.15107/rcub_technorep_6881 .

TY  - JOUR
AU  - Krkobabić, Ana
AU  - Radetić, Maja
AU  - Tseng, Hsiang-Han
AU  - Nunney, Tim S.
AU  - Tadić, Vanja
AU  - Ilić-Tomić, Tatjana
AU  - Marković, Darka
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5286
AB  - The demand for medical textiles in various forms with strong antimicrobial activity drastically increased during the COVID19 pandemic. In an attempt to tackle this issue and to develop antimicrobial textiles in more environmentally benign manner, a viscose fabric after coating with biopolymer chitosan has been impregnated with Ag- and Cu-based nanoparticles. Chitosan was applied in the presence and absence of cross-linker 1,2,3,4-butanetetracarboxylic acid (BTCA). In situ green synthesis of nanoparticles was performed using a Punica granatum (pomegranate) peel extract as a reducing and stabilizing agent. Formation of nanoparticles on the fiber surface was confirmed by FESEM. Elemental analysis by XPS showed the synthesized nanoparticles exist as AgCl and a mixture of Cu/CuO/Cu2O in the modified samples. Moreover, these nanoparticles appeared to be present not only on the sample surface but also buried within the fibers, as indicated by XPS mapping and depth profiling measurements. All impregnated fabrics exhibited excellent antifungal activity providing the maximum reduction of yeast Candida albicans colonies. Antibacterial activity was stronger against Gram-negative bacteria Escherichia coli than Gram-positive bacteria Staphylococcus aureus, and it was highly influenced by metal content. The fabrics impregnated with AgCl nanoparticles showed lower cytotoxicity towards human keratinocyte cells.
PB  - Elsevier B.V.
T2  - Applied Surface Science
T1  - Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract
SP  - 155612
VL  - 611
DO  - 10.1016/j.apsusc.2022.155612
ER  - 

@article{
author = "Krkobabić, Ana and Radetić, Maja and Tseng, Hsiang-Han and Nunney, Tim S. and Tadić, Vanja and Ilić-Tomić, Tatjana and Marković, Darka",
year = "2023",
abstract = "The demand for medical textiles in various forms with strong antimicrobial activity drastically increased during the COVID19 pandemic. In an attempt to tackle this issue and to develop antimicrobial textiles in more environmentally benign manner, a viscose fabric after coating with biopolymer chitosan has been impregnated with Ag- and Cu-based nanoparticles. Chitosan was applied in the presence and absence of cross-linker 1,2,3,4-butanetetracarboxylic acid (BTCA). In situ green synthesis of nanoparticles was performed using a Punica granatum (pomegranate) peel extract as a reducing and stabilizing agent. Formation of nanoparticles on the fiber surface was confirmed by FESEM. Elemental analysis by XPS showed the synthesized nanoparticles exist as AgCl and a mixture of Cu/CuO/Cu2O in the modified samples. Moreover, these nanoparticles appeared to be present not only on the sample surface but also buried within the fibers, as indicated by XPS mapping and depth profiling measurements. All impregnated fabrics exhibited excellent antifungal activity providing the maximum reduction of yeast Candida albicans colonies. Antibacterial activity was stronger against Gram-negative bacteria Escherichia coli than Gram-positive bacteria Staphylococcus aureus, and it was highly influenced by metal content. The fabrics impregnated with AgCl nanoparticles showed lower cytotoxicity towards human keratinocyte cells.",
publisher = "Elsevier B.V.",
journal = "Applied Surface Science",
title = "Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract",
pages = "155612",
volume = "611",
doi = "10.1016/j.apsusc.2022.155612"
}

Krkobabić, A., Radetić, M., Tseng, H., Nunney, T. S., Tadić, V., Ilić-Tomić, T.,& Marković, D.. (2023). Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract. in Applied Surface Science
Elsevier B.V.., 611, 155612.
https://doi.org/10.1016/j.apsusc.2022.155612

Krkobabić A, Radetić M, Tseng H, Nunney TS, Tadić V, Ilić-Tomić T, Marković D. Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract. in Applied Surface Science. 2023;611:155612.
doi:10.1016/j.apsusc.2022.155612 .

Krkobabić, Ana, Radetić, Maja, Tseng, Hsiang-Han, Nunney, Tim S., Tadić, Vanja, Ilić-Tomić, Tatjana, Marković, Darka, "Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract" in Applied Surface Science, 611 (2023):155612,
https://doi.org/10.1016/j.apsusc.2022.155612 . .

TY  - CONF
AU  - Lukić, Ivana
AU  - Milovanović, Stoja
AU  - Stamenić, Marko
AU  - Tadić, Vanja
AU  - Skala, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6985
AB  - Cannabis sativa is one of the oldest plant cultures, which has been used for centuries as a source of textile fibres, for paper production, as well as a source of bioactive compounds used in medicine. Although being restrained for a long time, its high potential, coupled with the progress in legislation and/or legalization in some regions of the world, led to the significant increase of scientific research towards isolation of cannabinoids, primarily a non-psychotropic cannabidiol (CBD), over the past couple decades. 
Beside cannabinoids, found only in cannabis plants, Cannabis sativa is characterized by very complex chemical composition with almost 500 constituents, including terpenes, flavonoids, phenolic compounds and fatty acids [2]. The co-presence of these numerous molecules in preparations obtained from Cannabis sativa may lead to a greater biological activity due to  the “entourage” or synergistic effect [3]. CBD has attracted considerable interest due to its numerous therapeutic properties and pharmacological activities [1]. However, cannabinoids are characterized by a low solubility in aqueous media and, as a consequence, a relatively poor bioavailability. Therefore, improvement of their stability, shelf-life and bioavailability, by incorporation into biocompatible carriers is still a challenge. 
As an answer to this issue, integrated process of supercritical fluid extraction (SFE) from Cannabis sativa and supercritical solvent impregnation (SSI) of obtained extract into starch aerogel was tested. Starch gels were prepared starting from hydrogel formed from an aqueous solution of cornstarch (1:10 w/v). During replacement of water with acetone, by successive increase of its concentration until 100%, acetogel was formed and further subjected to drying using supercritical CO2 (scCO2) at 45 °C and 8-10 MPa to obtain aerogel. For comparison, a part of acetogel was dried in the oven at 60 °C to obtain xerogel. Textural properties and morphology of prepared materials were determined by N2 adsorption–desorption isotherms and scanning electron microscopy (SEM) analysis. Aerogel with highest specific surface area of 208.6 m2/g, obtained at 45 °C and 10 MPa, was further used for extract impregnation. Integrated SFE-SSI process was performed at 40 °C and 10 MPa, conditions determined as optimal in the process of SFE of Cannabis sativa taking into account the yield of the obtained extract based on the composition, primarily regarding the CBD content. The amounts of impregnated extracts were 2.6 and 15.3% for xerogel and aerogel, respectively. The presence of the extract on the surface of impregnated samples was confirmed by FTIR analysis. Release study showed controlled release of extracts in PBS solution.
C3  - 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022
T1  - Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation
SP  - P-28
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6985
ER  - 

@conference{
author = "Lukić, Ivana and Milovanović, Stoja and Stamenić, Marko and Tadić, Vanja and Skala, Dejan",
year = "2022",
abstract = "Cannabis sativa is one of the oldest plant cultures, which has been used for centuries as a source of textile fibres, for paper production, as well as a source of bioactive compounds used in medicine. Although being restrained for a long time, its high potential, coupled with the progress in legislation and/or legalization in some regions of the world, led to the significant increase of scientific research towards isolation of cannabinoids, primarily a non-psychotropic cannabidiol (CBD), over the past couple decades. 
Beside cannabinoids, found only in cannabis plants, Cannabis sativa is characterized by very complex chemical composition with almost 500 constituents, including terpenes, flavonoids, phenolic compounds and fatty acids [2]. The co-presence of these numerous molecules in preparations obtained from Cannabis sativa may lead to a greater biological activity due to  the “entourage” or synergistic effect [3]. CBD has attracted considerable interest due to its numerous therapeutic properties and pharmacological activities [1]. However, cannabinoids are characterized by a low solubility in aqueous media and, as a consequence, a relatively poor bioavailability. Therefore, improvement of their stability, shelf-life and bioavailability, by incorporation into biocompatible carriers is still a challenge. 
As an answer to this issue, integrated process of supercritical fluid extraction (SFE) from Cannabis sativa and supercritical solvent impregnation (SSI) of obtained extract into starch aerogel was tested. Starch gels were prepared starting from hydrogel formed from an aqueous solution of cornstarch (1:10 w/v). During replacement of water with acetone, by successive increase of its concentration until 100%, acetogel was formed and further subjected to drying using supercritical CO2 (scCO2) at 45 °C and 8-10 MPa to obtain aerogel. For comparison, a part of acetogel was dried in the oven at 60 °C to obtain xerogel. Textural properties and morphology of prepared materials were determined by N2 adsorption–desorption isotherms and scanning electron microscopy (SEM) analysis. Aerogel with highest specific surface area of 208.6 m2/g, obtained at 45 °C and 10 MPa, was further used for extract impregnation. Integrated SFE-SSI process was performed at 40 °C and 10 MPa, conditions determined as optimal in the process of SFE of Cannabis sativa taking into account the yield of the obtained extract based on the composition, primarily regarding the CBD content. The amounts of impregnated extracts were 2.6 and 15.3% for xerogel and aerogel, respectively. The presence of the extract on the surface of impregnated samples was confirmed by FTIR analysis. Release study showed controlled release of extracts in PBS solution.",
journal = "2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022",
title = "Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation",
pages = "P-28",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6985"
}

Lukić, I., Milovanović, S., Stamenić, M., Tadić, V.,& Skala, D.. (2022). Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation. in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022, P-28.
https://hdl.handle.net/21.15107/rcub_technorep_6985

Lukić I, Milovanović S, Stamenić M, Tadić V, Skala D. Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation. in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022. 2022;:P-28.
https://hdl.handle.net/21.15107/rcub_technorep_6985 .

Lukić, Ivana, Milovanović, Stoja, Stamenić, Marko, Tadić, Vanja, Skala, Dejan, "Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation" in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022 (2022):P-28,
https://hdl.handle.net/21.15107/rcub_technorep_6985 .

TY  - CONF
AU  - Lukić, Ivana
AU  - Pajnik, Jelena
AU  - Milovanović, Stoja
AU  - Tadić, Vanja
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6905
AB  - Bilberries (Vaccinium myrtillus) are believed to have one of the highest antioxidant levels of all
common fruits and vegetables. As a source of antioxidants, they have been used in traditional
medicine to reduce inflammation and protect against diseases associated with oxidative stress, such
as cardiovascular disease, diabetes, and age-related cognitive decline, as well as a remedy for eye
conditions. The biological activity of bilberries is associated with the high content of anthocyanins,
a large group of water-soluble flavonoids. Besides the complex anthocyanin profile, bilberries also
contain other phenolic compounds including flavonols, tannins, and phenolic acids. Supercritical fluid
extraction (SFE) isa a green and environmentally friendly process based on the use of supercritical
fluids, most commonly CO2, as an alternative to organic solvents, to obtain solvent-free and highly
valuable plant extracts. Utilization of supercritical CO2 (scCO2) extraction also enables the preservation
of thermally labile compounds working at low temperatures, and good selectivity accomplished by
the appropriate choice of operating pressure and temperature. In this study, scCO2 extraction was
investigated as a method for isolating the extract from bilberry dried fruit. Extractions were performed
at temperature of 70 °C and pressures of 15 and 30 MPa, with and without the ethanol as co-solvent,
with the aim to optimize the yield of the obtained extract based on the composition. The obtained
extraction yields ranged from 1.2 to 7% and the highest yield was obtained, as expected, at a higher
pressure of 30 MPa. The effect of ethanol as co-solvent in the scCO2 extraction was studied with the
goal to modify scCO2 selectivity and enhance the solubility of polar substances. The results showed a
positive effect on the extraction yield, while total phenolic content (TPC) analysis revealed that extracts
obtained using co-solvent have higher TPC values. Chemical analysis of the obtained extracts was
performed in order to qualitatively evaluate their composition.
PB  - Zagreb : University, Faculty of Food Technology and Biotechnology
C3  - Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022
T1  - SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT
SP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6905
ER  - 

@conference{
author = "Lukić, Ivana and Pajnik, Jelena and Milovanović, Stoja and Tadić, Vanja",
year = "2022",
abstract = "Bilberries (Vaccinium myrtillus) are believed to have one of the highest antioxidant levels of all
common fruits and vegetables. As a source of antioxidants, they have been used in traditional
medicine to reduce inflammation and protect against diseases associated with oxidative stress, such
as cardiovascular disease, diabetes, and age-related cognitive decline, as well as a remedy for eye
conditions. The biological activity of bilberries is associated with the high content of anthocyanins,
a large group of water-soluble flavonoids. Besides the complex anthocyanin profile, bilberries also
contain other phenolic compounds including flavonols, tannins, and phenolic acids. Supercritical fluid
extraction (SFE) isa a green and environmentally friendly process based on the use of supercritical
fluids, most commonly CO2, as an alternative to organic solvents, to obtain solvent-free and highly
valuable plant extracts. Utilization of supercritical CO2 (scCO2) extraction also enables the preservation
of thermally labile compounds working at low temperatures, and good selectivity accomplished by
the appropriate choice of operating pressure and temperature. In this study, scCO2 extraction was
investigated as a method for isolating the extract from bilberry dried fruit. Extractions were performed
at temperature of 70 °C and pressures of 15 and 30 MPa, with and without the ethanol as co-solvent,
with the aim to optimize the yield of the obtained extract based on the composition. The obtained
extraction yields ranged from 1.2 to 7% and the highest yield was obtained, as expected, at a higher
pressure of 30 MPa. The effect of ethanol as co-solvent in the scCO2 extraction was studied with the
goal to modify scCO2 selectivity and enhance the solubility of polar substances. The results showed a
positive effect on the extraction yield, while total phenolic content (TPC) analysis revealed that extracts
obtained using co-solvent have higher TPC values. Chemical analysis of the obtained extracts was
performed in order to qualitatively evaluate their composition.",
publisher = "Zagreb : University, Faculty of Food Technology and Biotechnology",
journal = "Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022",
title = "SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT",
pages = "100",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6905"
}

Lukić, I., Pajnik, J., Milovanović, S.,& Tadić, V.. (2022). SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT. in Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022
Zagreb : University, Faculty of Food Technology and Biotechnology., 100.
https://hdl.handle.net/21.15107/rcub_technorep_6905

Lukić I, Pajnik J, Milovanović S, Tadić V. SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT. in Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022. 2022;:100.
https://hdl.handle.net/21.15107/rcub_technorep_6905 .

Lukić, Ivana, Pajnik, Jelena, Milovanović, Stoja, Tadić, Vanja, "SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT" in Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022 (2022):100,
https://hdl.handle.net/21.15107/rcub_technorep_6905 .

TY  - JOUR
AU  - Lukić, Ivana
AU  - Milovanović, Stoja
AU  - Pantić, Milica
AU  - Srbljak, Ivana
AU  - Djurić, Ana
AU  - Tadić, Vanja
AU  - Tyśkiewicz, Katarzyna
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5093
AB  - Extracts from Silybum marianum seeds have high industrial potential due to their application as phytopharmaceuticals and food. In order to optimize its production, conventional extraction (in Soxhlet apparatus using solvents ethanol and n-hexane) and supercritical fluid extraction (using CO2 at the pressure of 30 MPa and temperature of 40 °C with and without co-solvent ethanol, from oil-rich and defatted seeds) were compared. In addition, the effect of plant cultivation (years 2011 and 2019) and the effect of seeds and extract storage on the yield and quality of extracts were assessed. It was shown that unsaturated fatty acids (linoleic and oleic acids being most dominant) constituted 64–87% of obtained extracts. Content of α-tocopherol varied from 0.01 to 13 g/100 g, while total phenolic content was in the range from 13.2 to 104.2 gGAE/100 gextract. In vitro cytotoxic activity analysis confirmed that extracts obtained from defatted seeds showed activity against cancer cells. This study pointed out the important role of the cultivation year, selection of extraction technique and solvent, as well as storage on the extraction yield, chemical profile, and cytotoxic activity of extracts.
PB  - Academic Press
T2  - LWT
T1  - Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity
SP  - 113319
VL  - 160
DO  - 10.1016/j.lwt.2022.113319
ER  - 

@article{
author = "Lukić, Ivana and Milovanović, Stoja and Pantić, Milica and Srbljak, Ivana and Djurić, Ana and Tadić, Vanja and Tyśkiewicz, Katarzyna",
year = "2022",
abstract = "Extracts from Silybum marianum seeds have high industrial potential due to their application as phytopharmaceuticals and food. In order to optimize its production, conventional extraction (in Soxhlet apparatus using solvents ethanol and n-hexane) and supercritical fluid extraction (using CO2 at the pressure of 30 MPa and temperature of 40 °C with and without co-solvent ethanol, from oil-rich and defatted seeds) were compared. In addition, the effect of plant cultivation (years 2011 and 2019) and the effect of seeds and extract storage on the yield and quality of extracts were assessed. It was shown that unsaturated fatty acids (linoleic and oleic acids being most dominant) constituted 64–87% of obtained extracts. Content of α-tocopherol varied from 0.01 to 13 g/100 g, while total phenolic content was in the range from 13.2 to 104.2 gGAE/100 gextract. In vitro cytotoxic activity analysis confirmed that extracts obtained from defatted seeds showed activity against cancer cells. This study pointed out the important role of the cultivation year, selection of extraction technique and solvent, as well as storage on the extraction yield, chemical profile, and cytotoxic activity of extracts.",
publisher = "Academic Press",
journal = "LWT",
title = "Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity",
pages = "113319",
volume = "160",
doi = "10.1016/j.lwt.2022.113319"
}

Lukić, I., Milovanović, S., Pantić, M., Srbljak, I., Djurić, A., Tadić, V.,& Tyśkiewicz, K.. (2022). Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity. in LWT
Academic Press., 160, 113319.
https://doi.org/10.1016/j.lwt.2022.113319

Lukić I, Milovanović S, Pantić M, Srbljak I, Djurić A, Tadić V, Tyśkiewicz K. Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity. in LWT. 2022;160:113319.
doi:10.1016/j.lwt.2022.113319 .

Lukić, Ivana, Milovanović, Stoja, Pantić, Milica, Srbljak, Ivana, Djurić, Ana, Tadić, Vanja, Tyśkiewicz, Katarzyna, "Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity" in LWT, 160 (2022):113319,
https://doi.org/10.1016/j.lwt.2022.113319 . .

TY  - JOUR
AU  - Lukić, Ivana
AU  - Pajnik, Jelena
AU  - Nišavić, Jakov
AU  - Tadić, Vanja
AU  - Vági, Erika
AU  - Szekely, Edit
AU  - Žižović, Irena
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5113
AB  - The integrated supercritical fluid extraction–impregnation process (SFE-SSI) was performed to fabricate material with antiviral properties against the herpes simplex virus (HSV). Cotton gauze and starch/chitosan polymer films (SCF) were impregnated with components extracted from Melissa officinalis at 10 MPa and 40 °C using a green medium, supercritical carbon dioxide (scCO2). The influences of the processing mode regarding the flow of the supercritical fluid through the system, and the mass ratio of the plant material and the solid carrier, on the impregnation yield of M. officinalis extract were studied. The results revealed that the introduction of a fresh amount of CO2 into the system enabled the highest impregnation yield of 2.24% for cotton gauze and 8.71% for SCF. The presence of M. officinalis extract on the surface of both impregnated cotton gaze and SCF was confirmed by FTIR and GC analyses after the re-extraction of the impregnated samples. The M. officinalis impregnated materials showed a strong inhibitory effect against Bovine herpesvirus type 1 (BHV-1).
PB  - MDPI
T2  - Processes
T1  - Application of the Integrated Supercritical Fluid Extraction–Impregnation Process (SFE-SSI) for Development of Materials with Antiviral Properties
IS  - 4
SP  - 680
VL  - 10
DO  - 10.3390/pr10040680
ER  - 

@article{
author = "Lukić, Ivana and Pajnik, Jelena and Nišavić, Jakov and Tadić, Vanja and Vági, Erika and Szekely, Edit and Žižović, Irena",
year = "2022",
abstract = "The integrated supercritical fluid extraction–impregnation process (SFE-SSI) was performed to fabricate material with antiviral properties against the herpes simplex virus (HSV). Cotton gauze and starch/chitosan polymer films (SCF) were impregnated with components extracted from Melissa officinalis at 10 MPa and 40 °C using a green medium, supercritical carbon dioxide (scCO2). The influences of the processing mode regarding the flow of the supercritical fluid through the system, and the mass ratio of the plant material and the solid carrier, on the impregnation yield of M. officinalis extract were studied. The results revealed that the introduction of a fresh amount of CO2 into the system enabled the highest impregnation yield of 2.24% for cotton gauze and 8.71% for SCF. The presence of M. officinalis extract on the surface of both impregnated cotton gaze and SCF was confirmed by FTIR and GC analyses after the re-extraction of the impregnated samples. The M. officinalis impregnated materials showed a strong inhibitory effect against Bovine herpesvirus type 1 (BHV-1).",
publisher = "MDPI",
journal = "Processes",
title = "Application of the Integrated Supercritical Fluid Extraction–Impregnation Process (SFE-SSI) for Development of Materials with Antiviral Properties",
number = "4",
pages = "680",
volume = "10",
doi = "10.3390/pr10040680"
}

Lukić, I., Pajnik, J., Nišavić, J., Tadić, V., Vági, E., Szekely, E.,& Žižović, I.. (2022). Application of the Integrated Supercritical Fluid Extraction–Impregnation Process (SFE-SSI) for Development of Materials with Antiviral Properties. in Processes
MDPI., 10(4), 680.
https://doi.org/10.3390/pr10040680

Lukić I, Pajnik J, Nišavić J, Tadić V, Vági E, Szekely E, Žižović I. Application of the Integrated Supercritical Fluid Extraction–Impregnation Process (SFE-SSI) for Development of Materials with Antiviral Properties. in Processes. 2022;10(4):680.
doi:10.3390/pr10040680 .

Lukić, Ivana, Pajnik, Jelena, Nišavić, Jakov, Tadić, Vanja, Vági, Erika, Szekely, Edit, Žižović, Irena, "Application of the Integrated Supercritical Fluid Extraction–Impregnation Process (SFE-SSI) for Development of Materials with Antiviral Properties" in Processes, 10, no. 4 (2022):680,
https://doi.org/10.3390/pr10040680 . .

TY  - CONF
AU  - Krkobabić, Ana G.
AU  - Radetić, Maja M.
AU  - Tadić, Vanja M.
AU  - Ilić-Tomić, Tatjana R.
AU  - Marković, Darka D.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6880
AB  - Potrebe za medicinskim tekstilom koji poseduje antimikrobna svojstva kontinualno rastu.
U poslednje vreme se cine napori da se sinteza nanocestica Ag ostvari upotrebom zelenih
redukcionih sredstava, a pre svega biljnih ekstrakta. U ovom radu su nanocestice Ag
sintetisane na viskoznoj tkanini modifikovanoj biopolimerom hitozanom sa i bez prisustva
umreživaca 1,2,3,4-butantetrakarboksilne kiseline primenom ekstrakta kore nara kao
redukcionog sredstva. Uprkos razlici u sadržaju i velicini nanocestica Ag, oba uzorka
pokazuju odlicnu antibakterijsku aktivnost prema bakterijamaS. aureusi E. coli, a još bolju
antifungalnu aktivnost prema kvascu C. albicans. Pri tome je citotoksicnost ovih uzoraka
prema celijama keratinocita ljudske kože niska.
AB  - The needs for medical textiles with antimicrobial properties are continually growing.
Recently many efforts have been made to synthesize Ag nanoparticles using green
reducing agents, in particular plant extracts. In this study Ag nanoparticles were
synthesized on viscose rayon fabric modified with biopolymer chitosan with or without
crosslinker 1,2,3,4-tetrabutanecarboxylic acid by applying pomegranate peel extract as a
reducing agent. Despite the difference in content andsize of Ag nanoparticles both samples
showed excellent antibacterial activity against bacteria S. aureus and E. coli and even
better against yeast C. albicans. The cytotoxicity of these samples onhuman keratinocyte
cellswas low.
PB  - Beograd : Srpsko hemijsko društvo
C3  - Kratki izvodi radova ; Knjiga radova / 58. savetovanje Srpskog hemijskog društva, Beograd, 9. i 10. jun 2022. godine
T1  - Antimikrobna aktivnost viskozne tkanine modifikovane hitozanom i nanočesticama Ag
T1  - Antimicrobial activity of viscose rayon fabric modified with chitosan and Ag nanoparticles
SP  - 146
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6880
ER  - 

@conference{
author = "Krkobabić, Ana G. and Radetić, Maja M. and Tadić, Vanja M. and Ilić-Tomić, Tatjana R. and Marković, Darka D.",
year = "2022",
abstract = "Potrebe za medicinskim tekstilom koji poseduje antimikrobna svojstva kontinualno rastu.
U poslednje vreme se cine napori da se sinteza nanocestica Ag ostvari upotrebom zelenih
redukcionih sredstava, a pre svega biljnih ekstrakta. U ovom radu su nanocestice Ag
sintetisane na viskoznoj tkanini modifikovanoj biopolimerom hitozanom sa i bez prisustva
umreživaca 1,2,3,4-butantetrakarboksilne kiseline primenom ekstrakta kore nara kao
redukcionog sredstva. Uprkos razlici u sadržaju i velicini nanocestica Ag, oba uzorka
pokazuju odlicnu antibakterijsku aktivnost prema bakterijamaS. aureusi E. coli, a još bolju
antifungalnu aktivnost prema kvascu C. albicans. Pri tome je citotoksicnost ovih uzoraka
prema celijama keratinocita ljudske kože niska., The needs for medical textiles with antimicrobial properties are continually growing.
Recently many efforts have been made to synthesize Ag nanoparticles using green
reducing agents, in particular plant extracts. In this study Ag nanoparticles were
synthesized on viscose rayon fabric modified with biopolymer chitosan with or without
crosslinker 1,2,3,4-tetrabutanecarboxylic acid by applying pomegranate peel extract as a
reducing agent. Despite the difference in content andsize of Ag nanoparticles both samples
showed excellent antibacterial activity against bacteria S. aureus and E. coli and even
better against yeast C. albicans. The cytotoxicity of these samples onhuman keratinocyte
cellswas low.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "Kratki izvodi radova ; Knjiga radova / 58. savetovanje Srpskog hemijskog društva, Beograd, 9. i 10. jun 2022. godine",
title = "Antimikrobna aktivnost viskozne tkanine modifikovane hitozanom i nanočesticama Ag, Antimicrobial activity of viscose rayon fabric modified with chitosan and Ag nanoparticles",
pages = "146",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6880"
}

Krkobabić, A. G., Radetić, M. M., Tadić, V. M., Ilić-Tomić, T. R.,& Marković, D. D.. (2022). Antimikrobna aktivnost viskozne tkanine modifikovane hitozanom i nanočesticama Ag. in Kratki izvodi radova ; Knjiga radova / 58. savetovanje Srpskog hemijskog društva, Beograd, 9. i 10. jun 2022. godine
Beograd : Srpsko hemijsko društvo., 146.
https://hdl.handle.net/21.15107/rcub_technorep_6880

Krkobabić AG, Radetić MM, Tadić VM, Ilić-Tomić TR, Marković DD. Antimikrobna aktivnost viskozne tkanine modifikovane hitozanom i nanočesticama Ag. in Kratki izvodi radova ; Knjiga radova / 58. savetovanje Srpskog hemijskog društva, Beograd, 9. i 10. jun 2022. godine. 2022;:146.
https://hdl.handle.net/21.15107/rcub_technorep_6880 .

Krkobabić, Ana G., Radetić, Maja M., Tadić, Vanja M., Ilić-Tomić, Tatjana R., Marković, Darka D., "Antimikrobna aktivnost viskozne tkanine modifikovane hitozanom i nanočesticama Ag" in Kratki izvodi radova ; Knjiga radova / 58. savetovanje Srpskog hemijskog društva, Beograd, 9. i 10. jun 2022. godine (2022):146,
https://hdl.handle.net/21.15107/rcub_technorep_6880 .

TY  - JOUR
AU  - Krkobabić, Ana
AU  - Marković, Darka
AU  - Kovačević, Aleksandar
AU  - Tadić, Vanja
AU  - Radoičić, Marija
AU  - Barudžija, Tatjana
AU  - Ilic-Tomic, Tatjana
AU  - Radetić, Maja
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5130
AB  - The aim of this study was to develop antimicrobial nanocomposite textile material comprising of Cu-based nanostructures synthesized on oxidized cotton fabric using Arctostaphylos uva-ursi (L.) Spreng., Ericaceae (bearberry leaves) as a green reducing agent for adsorbed Cu2+-ions. In order to provide sufficient number of carboxyl groups for complexation with Cu2+-ions a two-step oxidation process with NaIO4 and NaClO2 was carried out. The influence of NaIO4 concentration on content of carboxyl groups and Cu-based nanoparticles was studied by FTIR and AAS. HPLC analysis identified the gallic acid known as a reducing agent in bearberry leaves extract. FESEM and XRD analyses revealed that using bearberry leaves extract and gallic acid solution as reducing agents led to a formation of spherical Cu2O/CuO nanoparticles and CuO nanosheets, respectively. These nanoparticles and nanosheets provided excellent antibacterial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus. Cytotoxicity on human keratinocyte cells was shown to depend on their copper content.
PB  - Korean Fiber Society
T2  - Fibers and Polymers
T1  - Antimicrobial Nanocomposites Based on Oxidized Cotton Fabric and in situ Biosynthesized Copper Oxides Nanostructures Using Bearberry Leaves Extract
EP  - 966
IS  - 4
SP  - 954
VL  - 23
DO  - 10.1007/s12221-022-4639-5
ER  - 

@article{
author = "Krkobabić, Ana and Marković, Darka and Kovačević, Aleksandar and Tadić, Vanja and Radoičić, Marija and Barudžija, Tatjana and Ilic-Tomic, Tatjana and Radetić, Maja",
year = "2022",
abstract = "The aim of this study was to develop antimicrobial nanocomposite textile material comprising of Cu-based nanostructures synthesized on oxidized cotton fabric using Arctostaphylos uva-ursi (L.) Spreng., Ericaceae (bearberry leaves) as a green reducing agent for adsorbed Cu2+-ions. In order to provide sufficient number of carboxyl groups for complexation with Cu2+-ions a two-step oxidation process with NaIO4 and NaClO2 was carried out. The influence of NaIO4 concentration on content of carboxyl groups and Cu-based nanoparticles was studied by FTIR and AAS. HPLC analysis identified the gallic acid known as a reducing agent in bearberry leaves extract. FESEM and XRD analyses revealed that using bearberry leaves extract and gallic acid solution as reducing agents led to a formation of spherical Cu2O/CuO nanoparticles and CuO nanosheets, respectively. These nanoparticles and nanosheets provided excellent antibacterial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus. Cytotoxicity on human keratinocyte cells was shown to depend on their copper content.",
publisher = "Korean Fiber Society",
journal = "Fibers and Polymers",
title = "Antimicrobial Nanocomposites Based on Oxidized Cotton Fabric and in situ Biosynthesized Copper Oxides Nanostructures Using Bearberry Leaves Extract",
pages = "966-954",
number = "4",
volume = "23",
doi = "10.1007/s12221-022-4639-5"
}

Krkobabić, A., Marković, D., Kovačević, A., Tadić, V., Radoičić, M., Barudžija, T., Ilic-Tomic, T.,& Radetić, M.. (2022). Antimicrobial Nanocomposites Based on Oxidized Cotton Fabric and in situ Biosynthesized Copper Oxides Nanostructures Using Bearberry Leaves Extract. in Fibers and Polymers
Korean Fiber Society., 23(4), 954-966.
https://doi.org/10.1007/s12221-022-4639-5

Krkobabić A, Marković D, Kovačević A, Tadić V, Radoičić M, Barudžija T, Ilic-Tomic T, Radetić M. Antimicrobial Nanocomposites Based on Oxidized Cotton Fabric and in situ Biosynthesized Copper Oxides Nanostructures Using Bearberry Leaves Extract. in Fibers and Polymers. 2022;23(4):954-966.
doi:10.1007/s12221-022-4639-5 .

Krkobabić, Ana, Marković, Darka, Kovačević, Aleksandar, Tadić, Vanja, Radoičić, Marija, Barudžija, Tatjana, Ilic-Tomic, Tatjana, Radetić, Maja, "Antimicrobial Nanocomposites Based on Oxidized Cotton Fabric and in situ Biosynthesized Copper Oxides Nanostructures Using Bearberry Leaves Extract" in Fibers and Polymers, 23, no. 4 (2022):954-966,
https://doi.org/10.1007/s12221-022-4639-5 . .

TY  - JOUR
AU  - Lović, Jelena
AU  - Bogdanović, Aleksandra
AU  - Tadić, Vanja
AU  - Mijin, Dušan
AU  - Vuković, Dragan
AU  - Petrović, Slobodan D.
AU  - Avramov-Ivić, Milka
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5191
AB  - Duloxetine hydrochloride (DUL) is effective in treating depression, and was examined for electroanalytical purposes. The DULstandard was investigated by cyclic voltammetry (CV) and determined using differential pulse voltammetry (DPV) via its electrooxidationat the Au electrode in 0.05 M NaHCO3. DPV showed a linear dependency of the anodic peak currents vs DUL standardconcentrations in the range from 0.1 to 3.33 μg ml−1 with the values of the limit of detection (LOD) and the limit of quantification(LOQ): 0.133 and 0.667 μg ml−1, respectively. Using the constructed and validated calibration curve, the values of unknown DULconcentrations in both Taita® tablets and in human serum spiked with the standard were determined. The number of protons andelectrons transferred was calculated and possible reaction mechanisms taking place on the surface of both electrodes wereproposed. The Au electrode exhibited a better sensitivity and a wider range of current vs concentration linear dependency for DULquantitative determination than the GC electrode. The study of DUL degradation showed that at the Au and GC electrodes, after4.5 h of potential cycling, degradation occurs, giving formaldehyde as a product, which was confirmed by high performance liquidchromatography (HPLC)
PB  - The Electrochemical Society
T2  - Journal of The Electrochemical Society
T1  - Electrochemical Behavior of Duloxetine Hydrochloride at Au and GC Solid Electrodes: Its Quantitative Determination and Degradation
IS  - 7
SP  - 076507
VL  - 169
DO  - 10.1149/1945-7111/ac7e74
ER  - 

@article{
author = "Lović, Jelena and Bogdanović, Aleksandra and Tadić, Vanja and Mijin, Dušan and Vuković, Dragan and Petrović, Slobodan D. and Avramov-Ivić, Milka",
year = "2022",
abstract = "Duloxetine hydrochloride (DUL) is effective in treating depression, and was examined for electroanalytical purposes. The DULstandard was investigated by cyclic voltammetry (CV) and determined using differential pulse voltammetry (DPV) via its electrooxidationat the Au electrode in 0.05 M NaHCO3. DPV showed a linear dependency of the anodic peak currents vs DUL standardconcentrations in the range from 0.1 to 3.33 μg ml−1 with the values of the limit of detection (LOD) and the limit of quantification(LOQ): 0.133 and 0.667 μg ml−1, respectively. Using the constructed and validated calibration curve, the values of unknown DULconcentrations in both Taita® tablets and in human serum spiked with the standard were determined. The number of protons andelectrons transferred was calculated and possible reaction mechanisms taking place on the surface of both electrodes wereproposed. The Au electrode exhibited a better sensitivity and a wider range of current vs concentration linear dependency for DULquantitative determination than the GC electrode. The study of DUL degradation showed that at the Au and GC electrodes, after4.5 h of potential cycling, degradation occurs, giving formaldehyde as a product, which was confirmed by high performance liquidchromatography (HPLC)",
publisher = "The Electrochemical Society",
journal = "Journal of The Electrochemical Society",
title = "Electrochemical Behavior of Duloxetine Hydrochloride at Au and GC Solid Electrodes: Its Quantitative Determination and Degradation",
number = "7",
pages = "076507",
volume = "169",
doi = "10.1149/1945-7111/ac7e74"
}

Lović, J., Bogdanović, A., Tadić, V., Mijin, D., Vuković, D., Petrović, S. D.,& Avramov-Ivić, M.. (2022). Electrochemical Behavior of Duloxetine Hydrochloride at Au and GC Solid Electrodes: Its Quantitative Determination and Degradation. in Journal of The Electrochemical Society
The Electrochemical Society., 169(7), 076507.
https://doi.org/10.1149/1945-7111/ac7e74

Lović J, Bogdanović A, Tadić V, Mijin D, Vuković D, Petrović SD, Avramov-Ivić M. Electrochemical Behavior of Duloxetine Hydrochloride at Au and GC Solid Electrodes: Its Quantitative Determination and Degradation. in Journal of The Electrochemical Society. 2022;169(7):076507.
doi:10.1149/1945-7111/ac7e74 .

Lović, Jelena, Bogdanović, Aleksandra, Tadić, Vanja, Mijin, Dušan, Vuković, Dragan, Petrović, Slobodan D., Avramov-Ivić, Milka, "Electrochemical Behavior of Duloxetine Hydrochloride at Au and GC Solid Electrodes: Its Quantitative Determination and Degradation" in Journal of The Electrochemical Society, 169, no. 7 (2022):076507,
https://doi.org/10.1149/1945-7111/ac7e74 . .

TY  - BOOK
AU  - Tadić, Vanja
AU  - Maksimović, Svetolik
AU  - Nešić, Ivana
AU  - Žižović, Irena
AU  - Mišić, Dušan
AU  - Milovanović, Stoja
AU  - Žugić, Ana
AU  - Aksentijević, Ksenija
AU  - Stanković, Milica
AU  - Ivanović, Jasna
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6876
PB  - Niš : Galaksijanis
T1  - Smilje od tradicionalne do naučno zasnovane primene
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6876
ER  - 

@book{
author = "Tadić, Vanja and Maksimović, Svetolik and Nešić, Ivana and Žižović, Irena and Mišić, Dušan and Milovanović, Stoja and Žugić, Ana and Aksentijević, Ksenija and Stanković, Milica and Ivanović, Jasna",
year = "2021",
publisher = "Niš : Galaksijanis",
title = "Smilje od tradicionalne do naučno zasnovane primene",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6876"
}

Tadić, V., Maksimović, S., Nešić, I., Žižović, I., Mišić, D., Milovanović, S., Žugić, A., Aksentijević, K., Stanković, M.,& Ivanović, J.. (2021). Smilje od tradicionalne do naučno zasnovane primene. 
Niš : Galaksijanis..
https://hdl.handle.net/21.15107/rcub_technorep_6876

Tadić V, Maksimović S, Nešić I, Žižović I, Mišić D, Milovanović S, Žugić A, Aksentijević K, Stanković M, Ivanović J. Smilje od tradicionalne do naučno zasnovane primene. 2021;.
https://hdl.handle.net/21.15107/rcub_technorep_6876 .

Tadić, Vanja, Maksimović, Svetolik, Nešić, Ivana, Žižović, Irena, Mišić, Dušan, Milovanović, Stoja, Žugić, Ana, Aksentijević, Ksenija, Stanković, Milica, Ivanović, Jasna, "Smilje od tradicionalne do naučno zasnovane primene" (2021),
https://hdl.handle.net/21.15107/rcub_technorep_6876 .

TY  - CONF
AU  - Milovanović, Stoja
AU  - Lukić, Ivana
AU  - Tadić, Vanja
AU  - Frank, Almut
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6893
AB  - Supercritical CO2 impregnation (SCI) process allows incorporation of bioactive components into solid carriers in an environmentally friendly manner. In recent years, SCI was highlighted as a promising method for preparation of added value materials that can protect bioactive components from environment (sun light, air, moisture etc.), stabilise them, and increase their shelf life. SCI process is also suitable for preparation of material with controlled release of bioactive component. Taking into account the above-mentioned, SCI process was employed in this study for incorporation of hemp seed oil (HSO) into biocompatible polymers (starch xerogel, starch aerogel, α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin). Given that efficiency of SCI depends on process conditions and affinity of bioactive component towards scCO2 and polymer, it is necessary to optimise process for every individual system. Proposed SCI process was performed at pressure of 30 MPa and temperature of 40 °C during 5 h and 18 h. FTIR analysis confirmed that scCO2 did not have effect on polymer composition nor it remained in polymer after process. Additionally, FTIR and UV-Vis analysis showed that HSO is present in all tested polymers after SCI process. It was shown that impregnation of HSO is highly dependent on selection of polymeric carrier (its chemical composition and morphology) and operating time employed, resulting in loadings from 0.5% to 40%. Chemical analysis showed that HSO is rich in unsaturated fatty acids especially linoleic acid (54-60%) and α-linolenic acid (17-20%). These essential fatty acids have well-established health benefits including protection against cardiovascular, neurodegenerative and inflammatory diseases. Proposed SCI process gave guidelines for development of added-value materials that are solvent free and can be used as phytopharmaceuticals.
C3  - Book of Abstracts / 1st GREENERING International Conference, COST Action GREENERING CA 18224 Green Chemical Engineering Network towards upscaling sustainable processes, 15th - 16th February 2021
T1  - Supercritical CO2 impregnation - selection of suitable polymeric carrier for hemp seed oil
SP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6893
ER  - 

@conference{
author = "Milovanović, Stoja and Lukić, Ivana and Tadić, Vanja and Frank, Almut",
year = "2021",
abstract = "Supercritical CO2 impregnation (SCI) process allows incorporation of bioactive components into solid carriers in an environmentally friendly manner. In recent years, SCI was highlighted as a promising method for preparation of added value materials that can protect bioactive components from environment (sun light, air, moisture etc.), stabilise them, and increase their shelf life. SCI process is also suitable for preparation of material with controlled release of bioactive component. Taking into account the above-mentioned, SCI process was employed in this study for incorporation of hemp seed oil (HSO) into biocompatible polymers (starch xerogel, starch aerogel, α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin). Given that efficiency of SCI depends on process conditions and affinity of bioactive component towards scCO2 and polymer, it is necessary to optimise process for every individual system. Proposed SCI process was performed at pressure of 30 MPa and temperature of 40 °C during 5 h and 18 h. FTIR analysis confirmed that scCO2 did not have effect on polymer composition nor it remained in polymer after process. Additionally, FTIR and UV-Vis analysis showed that HSO is present in all tested polymers after SCI process. It was shown that impregnation of HSO is highly dependent on selection of polymeric carrier (its chemical composition and morphology) and operating time employed, resulting in loadings from 0.5% to 40%. Chemical analysis showed that HSO is rich in unsaturated fatty acids especially linoleic acid (54-60%) and α-linolenic acid (17-20%). These essential fatty acids have well-established health benefits including protection against cardiovascular, neurodegenerative and inflammatory diseases. Proposed SCI process gave guidelines for development of added-value materials that are solvent free and can be used as phytopharmaceuticals.",
journal = "Book of Abstracts / 1st GREENERING International Conference, COST Action GREENERING CA 18224 Green Chemical Engineering Network towards upscaling sustainable processes, 15th - 16th February 2021",
title = "Supercritical CO2 impregnation - selection of suitable polymeric carrier for hemp seed oil",
pages = "47",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6893"
}

Milovanović, S., Lukić, I., Tadić, V.,& Frank, A.. (2021). Supercritical CO2 impregnation - selection of suitable polymeric carrier for hemp seed oil. in Book of Abstracts / 1st GREENERING International Conference, COST Action GREENERING CA 18224 Green Chemical Engineering Network towards upscaling sustainable processes, 15th - 16th February 2021, 47.
https://hdl.handle.net/21.15107/rcub_technorep_6893

Milovanović S, Lukić I, Tadić V, Frank A. Supercritical CO2 impregnation - selection of suitable polymeric carrier for hemp seed oil. in Book of Abstracts / 1st GREENERING International Conference, COST Action GREENERING CA 18224 Green Chemical Engineering Network towards upscaling sustainable processes, 15th - 16th February 2021. 2021;:47.
https://hdl.handle.net/21.15107/rcub_technorep_6893 .

Milovanović, Stoja, Lukić, Ivana, Tadić, Vanja, Frank, Almut, "Supercritical CO2 impregnation - selection of suitable polymeric carrier for hemp seed oil" in Book of Abstracts / 1st GREENERING International Conference, COST Action GREENERING CA 18224 Green Chemical Engineering Network towards upscaling sustainable processes, 15th - 16th February 2021 (2021):47,
https://hdl.handle.net/21.15107/rcub_technorep_6893 .

TY  - JOUR
AU  - Milovanović, Ivan
AU  - Zengin, Gokhan
AU  - Maksimović, Svetolik
AU  - Tadić, Vanja
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/17
AB  - Background This study provides an insight into the impact of ultrasound-assisted extraction with water as solvent (UAEW) and extraction by supercritical carbon dioxide (SC-CO2) with 5% EtOH on antioxidant and enzyme inhibitory activity in regard to the chemical profile of the edible and medicinal mushroom,Pleurotus pulmonarius. Results Extraction efficiency was between 0.36% and 63.32%, depending on the extraction technique. The main compounds in the extracts were fatty acids. Supercritical CO(2)extraction with co-solvent was the most suitable method for obtaining extracts that were rich in ergosterol content, reaching a value of 40.1 mg g(-1). The UAEW of crude mushroom powder ensured the highest yield, as well as the extracts with best antioxidative activity. The measurements of enzyme inhibitory activity revealed that all types of investigated extracts exhibited only tyrosinase and amylase inhibition at a significant level. Conclusion Based on our results, the extraction methods significantly affected the chemical profile and bioactivity ofP. pulmonarius.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Supercritical and ultrasound-assisted extracts fromPleurotus pulmonariusmushroom: chemical profiles, antioxidative, and enzyme-inhibitory properties
EP  - 2293
IS  - 6
SP  - 2284
VL  - 101
DO  - 10.1002/jsfa.10849
ER  - 

@article{
author = "Milovanović, Ivan and Zengin, Gokhan and Maksimović, Svetolik and Tadić, Vanja",
year = "2021",
abstract = "Background This study provides an insight into the impact of ultrasound-assisted extraction with water as solvent (UAEW) and extraction by supercritical carbon dioxide (SC-CO2) with 5% EtOH on antioxidant and enzyme inhibitory activity in regard to the chemical profile of the edible and medicinal mushroom,Pleurotus pulmonarius. Results Extraction efficiency was between 0.36% and 63.32%, depending on the extraction technique. The main compounds in the extracts were fatty acids. Supercritical CO(2)extraction with co-solvent was the most suitable method for obtaining extracts that were rich in ergosterol content, reaching a value of 40.1 mg g(-1). The UAEW of crude mushroom powder ensured the highest yield, as well as the extracts with best antioxidative activity. The measurements of enzyme inhibitory activity revealed that all types of investigated extracts exhibited only tyrosinase and amylase inhibition at a significant level. Conclusion Based on our results, the extraction methods significantly affected the chemical profile and bioactivity ofP. pulmonarius.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Supercritical and ultrasound-assisted extracts fromPleurotus pulmonariusmushroom: chemical profiles, antioxidative, and enzyme-inhibitory properties",
pages = "2293-2284",
number = "6",
volume = "101",
doi = "10.1002/jsfa.10849"
}

Milovanović, I., Zengin, G., Maksimović, S.,& Tadić, V.. (2021). Supercritical and ultrasound-assisted extracts fromPleurotus pulmonariusmushroom: chemical profiles, antioxidative, and enzyme-inhibitory properties. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 101(6), 2284-2293.
https://doi.org/10.1002/jsfa.10849

Milovanović I, Zengin G, Maksimović S, Tadić V. Supercritical and ultrasound-assisted extracts fromPleurotus pulmonariusmushroom: chemical profiles, antioxidative, and enzyme-inhibitory properties. in Journal of the Science of Food and Agriculture. 2021;101(6):2284-2293.
doi:10.1002/jsfa.10849 .

Milovanović, Ivan, Zengin, Gokhan, Maksimović, Svetolik, Tadić, Vanja, "Supercritical and ultrasound-assisted extracts fromPleurotus pulmonariusmushroom: chemical profiles, antioxidative, and enzyme-inhibitory properties" in Journal of the Science of Food and Agriculture, 101, no. 6 (2021):2284-2293,
https://doi.org/10.1002/jsfa.10849 . .

TY  - CONF
AU  - Krkobabić, Ana
AU  - Marković, Darka
AU  - Tadić, Vanja
AU  - Radoičić, Marija
AU  - Radetić, Maja
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6879
AB  - U ovom radu diskutovana je mogućnost sinteze nanolistića na bazi Cu na pamučnoj tkanini koji bi
pružili željeni nivo antimikrobne aktivnosti. Da bi se obezbedio dovoljan broj karboksilnih grupa za
vezivanje Cu2+
-jona iz rastvora soli prekursora pamučna tkanina je selektivno oksidisana u
dvostepenom procesu sa NaIO4 i NaClO2. Sorbovani Cu2+
-joni su redukovani galnom kiselinom.
FESEM i XRD analize su pokazale da nanolistići CuO organizovani u fine slojeve ravnomerno
pokrivaju površinu vlakana. Što je veća početna koncentracija NaIO4, veći je broj karboksilnih
grupa i posledično količina sintetisanih nanolistića CuO. Razvijeni tekstilni nanokompoziti su
pokazali odličnu antibakterijsku aktivnost prema Gram-negativnoj bakteriji Escherichia Coli i
Gram-pozitivnoj bakteriji Staphylococcus aureus, kao i zadovoljavajuću antifungalnu aktivnost
prema kvascu Candida albicans.
AB  - This study discussed the possibility of synthesizing Cu-based nanosheets on cotton fabric which could provide desired level of antimicrobial activity. In order to ensure a sufficient number of carboxyl groups that could efficiently bind Cu2+-ions from precursor salt solution, cotton fabric was selectively oxidized in two-step process with NaIO4 and NaClO2. Sorbed Cu2+-ions were reduced with gallic acid. FESEM and XRD analyses revealed that CuO nanosheets organized in fine layers evenly covered the surface of cotton fibers. The larger the initial concentration of NaIO4, the larger the number of carboxyl groups and consequently the amounts of synthesized CuO nanosheets. Fabricated textile nanocomposites exhibited excellent antibacterial activity against Gram-negative bacterium Escherichia Coli and Gram-positive bacterium Staphylococcus aureus as well as satisfactory antifungal activity against yeast Candida albicans.
PB  - Beograd : Srpsko hemijsko društvo
C3  - Kratki izvodi radova ; Knjiga radova / 57. savetovanje Srpskog hemijskog društva, Kragujevac 18. i 19. juni 2021
T1  - Antimikrobna aktivnost nanolistića CuO in situ sintetisanih na pamučnoj tkanini prethodno oksidisanoj sa NaIO4/NaClO2
T1  - Antimicrobial activity of CuO nanosheets in situ synthesized on cotton fabric previously oxidized with NaIO4/NaClO2
SP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6879
ER  - 

@conference{
author = "Krkobabić, Ana and Marković, Darka and Tadić, Vanja and Radoičić, Marija and Radetić, Maja",
year = "2021",
abstract = "U ovom radu diskutovana je mogućnost sinteze nanolistića na bazi Cu na pamučnoj tkanini koji bi
pružili željeni nivo antimikrobne aktivnosti. Da bi se obezbedio dovoljan broj karboksilnih grupa za
vezivanje Cu2+
-jona iz rastvora soli prekursora pamučna tkanina je selektivno oksidisana u
dvostepenom procesu sa NaIO4 i NaClO2. Sorbovani Cu2+
-joni su redukovani galnom kiselinom.
FESEM i XRD analize su pokazale da nanolistići CuO organizovani u fine slojeve ravnomerno
pokrivaju površinu vlakana. Što je veća početna koncentracija NaIO4, veći je broj karboksilnih
grupa i posledično količina sintetisanih nanolistića CuO. Razvijeni tekstilni nanokompoziti su
pokazali odličnu antibakterijsku aktivnost prema Gram-negativnoj bakteriji Escherichia Coli i
Gram-pozitivnoj bakteriji Staphylococcus aureus, kao i zadovoljavajuću antifungalnu aktivnost
prema kvascu Candida albicans., This study discussed the possibility of synthesizing Cu-based nanosheets on cotton fabric which could provide desired level of antimicrobial activity. In order to ensure a sufficient number of carboxyl groups that could efficiently bind Cu2+-ions from precursor salt solution, cotton fabric was selectively oxidized in two-step process with NaIO4 and NaClO2. Sorbed Cu2+-ions were reduced with gallic acid. FESEM and XRD analyses revealed that CuO nanosheets organized in fine layers evenly covered the surface of cotton fibers. The larger the initial concentration of NaIO4, the larger the number of carboxyl groups and consequently the amounts of synthesized CuO nanosheets. Fabricated textile nanocomposites exhibited excellent antibacterial activity against Gram-negative bacterium Escherichia Coli and Gram-positive bacterium Staphylococcus aureus as well as satisfactory antifungal activity against yeast Candida albicans.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "Kratki izvodi radova ; Knjiga radova / 57. savetovanje Srpskog hemijskog društva, Kragujevac 18. i 19. juni 2021",
title = "Antimikrobna aktivnost nanolistića CuO in situ sintetisanih na pamučnoj tkanini prethodno oksidisanoj sa NaIO4/NaClO2, Antimicrobial activity of CuO nanosheets in situ synthesized on cotton fabric previously oxidized with NaIO4/NaClO2",
pages = "51",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6879"
}

Krkobabić, A., Marković, D., Tadić, V., Radoičić, M.,& Radetić, M.. (2021). Antimikrobna aktivnost nanolistića CuO in situ sintetisanih na pamučnoj tkanini prethodno oksidisanoj sa NaIO4/NaClO2. in Kratki izvodi radova ; Knjiga radova / 57. savetovanje Srpskog hemijskog društva, Kragujevac 18. i 19. juni 2021
Beograd : Srpsko hemijsko društvo., 51.
https://hdl.handle.net/21.15107/rcub_technorep_6879

Krkobabić A, Marković D, Tadić V, Radoičić M, Radetić M. Antimikrobna aktivnost nanolistića CuO in situ sintetisanih na pamučnoj tkanini prethodno oksidisanoj sa NaIO4/NaClO2. in Kratki izvodi radova ; Knjiga radova / 57. savetovanje Srpskog hemijskog društva, Kragujevac 18. i 19. juni 2021. 2021;:51.
https://hdl.handle.net/21.15107/rcub_technorep_6879 .

Krkobabić, Ana, Marković, Darka, Tadić, Vanja, Radoičić, Marija, Radetić, Maja, "Antimikrobna aktivnost nanolistića CuO in situ sintetisanih na pamučnoj tkanini prethodno oksidisanoj sa NaIO4/NaClO2" in Kratki izvodi radova ; Knjiga radova / 57. savetovanje Srpskog hemijskog društva, Kragujevac 18. i 19. juni 2021 (2021):51,
https://hdl.handle.net/21.15107/rcub_technorep_6879 .

TY  - CONF
AU  - Marković, Darka
AU  - Kovačević, Aleksandar
AU  - Tadić, Vanja
AU  - Radoičić, Marija
AU  - Barudžija, Tanja
AU  - Radetić, Maja
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6864
AB  - This study discusses the antimicrobial activity of cotton (CO) fabric oxidized with NaIO4/NaClO2
prior to in situ synthesis of Cu-based nanoparticles (NPs). Carboxyl groups generated by oxidation
of cellulose were utilized for efficient binding of Cu2+-ions from the solution of CuSO4 which
were further reduced by extract of Arctostaphylos uva-ursi (L.) Spreng., Ericaceae (bearberry
leaves) as a green reducing agent. A number of free carboxyl groups was highly affected by the
initial concentration of NaIO4 solution. Color yield of impregnated fabrics varied with amounts of
synthesized Cu-based NPs. Fabricated samples provided excellent antibacterial activity and controlled
release of Cu2+-ions.
PB  - Portugal : University of Minho
C3  - Book of abstracts / Unfolding the future, 20th World Textile Conference AUTEX 2021, 5 - 9 September 2021, Guimarães, Portugal
T1  - Аntimicrobial activity of oxidized cotton fabric impregnated with Cu-based nanoparticles
EP  - 103
SP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6864
ER  - 

@conference{
author = "Marković, Darka and Kovačević, Aleksandar and Tadić, Vanja and Radoičić, Marija and Barudžija, Tanja and Radetić, Maja",
year = "2021",
abstract = "This study discusses the antimicrobial activity of cotton (CO) fabric oxidized with NaIO4/NaClO2
prior to in situ synthesis of Cu-based nanoparticles (NPs). Carboxyl groups generated by oxidation
of cellulose were utilized for efficient binding of Cu2+-ions from the solution of CuSO4 which
were further reduced by extract of Arctostaphylos uva-ursi (L.) Spreng., Ericaceae (bearberry
leaves) as a green reducing agent. A number of free carboxyl groups was highly affected by the
initial concentration of NaIO4 solution. Color yield of impregnated fabrics varied with amounts of
synthesized Cu-based NPs. Fabricated samples provided excellent antibacterial activity and controlled
release of Cu2+-ions.",
publisher = "Portugal : University of Minho",
journal = "Book of abstracts / Unfolding the future, 20th World Textile Conference AUTEX 2021, 5 - 9 September 2021, Guimarães, Portugal",
title = "Аntimicrobial activity of oxidized cotton fabric impregnated with Cu-based nanoparticles",
pages = "103-102",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6864"
}

Marković, D., Kovačević, A., Tadić, V., Radoičić, M., Barudžija, T.,& Radetić, M.. (2021). Аntimicrobial activity of oxidized cotton fabric impregnated with Cu-based nanoparticles. in Book of abstracts / Unfolding the future, 20th World Textile Conference AUTEX 2021, 5 - 9 September 2021, Guimarães, Portugal
Portugal : University of Minho., 102-103.
https://hdl.handle.net/21.15107/rcub_technorep_6864

Marković D, Kovačević A, Tadić V, Radoičić M, Barudžija T, Radetić M. Аntimicrobial activity of oxidized cotton fabric impregnated with Cu-based nanoparticles. in Book of abstracts / Unfolding the future, 20th World Textile Conference AUTEX 2021, 5 - 9 September 2021, Guimarães, Portugal. 2021;:102-103.
https://hdl.handle.net/21.15107/rcub_technorep_6864 .

Marković, Darka, Kovačević, Aleksandar, Tadić, Vanja, Radoičić, Marija, Barudžija, Tanja, Radetić, Maja, "Аntimicrobial activity of oxidized cotton fabric impregnated with Cu-based nanoparticles" in Book of abstracts / Unfolding the future, 20th World Textile Conference AUTEX 2021, 5 - 9 September 2021, Guimarães, Portugal (2021):102-103,
https://hdl.handle.net/21.15107/rcub_technorep_6864 .

TY  - JOUR
AU  - Bogdanović, Aleksandra
AU  - Tadić, Vanja
AU  - Petrović, Slobodan
AU  - Skala, Dejan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4543
AB  - Supercritical CO2 extraction was applied on fenugreek (Trigonella foenum-graecum L., Fabaceae) seeds with the aim to define optimal process conditions to obtain the maximal extract yields content of steroidal sapogenins. Central composite rotatable design (CCRD) combined with response surface methodology (RSM) was applied to determine optimal process conditions defined by the influence and interaction of pressure, temperature and time of extraction through consumption of SC CO2. Optimization experiments revealed that the pressure of 24.73 MPa, the temperature of 38.2 degrees C and the consumption of SC CO2 of 19.24 g/g(DM) were the best process conditions enabling the maximal yield of extract and gain of the highest content of sapogenins. The optimal values of pressure and temperature defined SC CO2 density of 885.47 kg/m(3), which provided the maximal yield of the extract with the highest content of steroidal sapogenins. The achieved yield of extract at these conditions was 0.073g/g(DM), with 0.774 mg/g(DM) diosgenin, 0.477 mg/g(DM) protodioscin, 0.713 mg/g(DM) sarsapogenin and 0.205 mg/g(DM) oleanolic and ursolic acid with a significant quantity of 2.475 mg/g(DM) in the obtained extract.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Supercritical CO2 extraction of steroidal sapogenins from fenugreek (tngonella foenum-graecum l.) Seed
EP  - 182
IS  - 2
SP  - 171
VL  - 26
DO  - 10.2298/CICEQ191001036B
ER  - 

@article{
author = "Bogdanović, Aleksandra and Tadić, Vanja and Petrović, Slobodan and Skala, Dejan",
year = "2020",
abstract = "Supercritical CO2 extraction was applied on fenugreek (Trigonella foenum-graecum L., Fabaceae) seeds with the aim to define optimal process conditions to obtain the maximal extract yields content of steroidal sapogenins. Central composite rotatable design (CCRD) combined with response surface methodology (RSM) was applied to determine optimal process conditions defined by the influence and interaction of pressure, temperature and time of extraction through consumption of SC CO2. Optimization experiments revealed that the pressure of 24.73 MPa, the temperature of 38.2 degrees C and the consumption of SC CO2 of 19.24 g/g(DM) were the best process conditions enabling the maximal yield of extract and gain of the highest content of sapogenins. The optimal values of pressure and temperature defined SC CO2 density of 885.47 kg/m(3), which provided the maximal yield of the extract with the highest content of steroidal sapogenins. The achieved yield of extract at these conditions was 0.073g/g(DM), with 0.774 mg/g(DM) diosgenin, 0.477 mg/g(DM) protodioscin, 0.713 mg/g(DM) sarsapogenin and 0.205 mg/g(DM) oleanolic and ursolic acid with a significant quantity of 2.475 mg/g(DM) in the obtained extract.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Supercritical CO2 extraction of steroidal sapogenins from fenugreek (tngonella foenum-graecum l.) Seed",
pages = "182-171",
number = "2",
volume = "26",
doi = "10.2298/CICEQ191001036B"
}

Bogdanović, A., Tadić, V., Petrović, S.,& Skala, D.. (2020). Supercritical CO2 extraction of steroidal sapogenins from fenugreek (tngonella foenum-graecum l.) Seed. in Chemical Industry & Chemical Engineering Quarterly
Savez hemijskih inženjera, Beograd., 26(2), 171-182.
https://doi.org/10.2298/CICEQ191001036B

Bogdanović A, Tadić V, Petrović S, Skala D. Supercritical CO2 extraction of steroidal sapogenins from fenugreek (tngonella foenum-graecum l.) Seed. in Chemical Industry & Chemical Engineering Quarterly. 2020;26(2):171-182.
doi:10.2298/CICEQ191001036B .

Bogdanović, Aleksandra, Tadić, Vanja, Petrović, Slobodan, Skala, Dejan, "Supercritical CO2 extraction of steroidal sapogenins from fenugreek (tngonella foenum-graecum l.) Seed" in Chemical Industry & Chemical Engineering Quarterly, 26, no. 2 (2020):171-182,
https://doi.org/10.2298/CICEQ191001036B . .