TY  - JOUR
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana D.
AU  - Ota, Ajda
AU  - Đorđević, Verica B.
AU  - Šavikin, Katarina P.
AU  - Nedović, Viktor A.
AU  - Poklar Ulrih, Nataša
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5942
AB  - The aim of the present study was the examination of the impact of β-sitosterol and gentisic acid on the characteristics of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine(DPPC) liposomal particles: (a) bilayer permeability (fluorescence spectroscopy),(b) particle size, polydispersity index (PDI) and zeta potential (photon correlation spectroscopy) and (c) thermal properties (differential scanning calorimetry).β-sitosterol induced the increase of liposomal bilayer rigidity, due to rearranging of the phospholipid chains, while gentisic acid enhanced the membrane fluidity, due to the reduced orderliness and the increase of phospholipid dynamics. The inclusion of β-sitosterol in liposomes caused a significant increase in particle diameter and PDI, while the encapsulation of gentisic acid did not have influence on particle size distribution. Apart from that, the presence ofβ-sitosterol resulted in the significant zeta potential increase, and thus a better stability of liposomal spheres(in the absence and in the presence of gentisic acid). β-sitosterol decreased main transition temperature (Tm) and phase transition enthalpy (∆H), and caused the disappearance of the pre-transition peak as well, whereas the presence of gentisic acid produced a slight decrease in Tm and increase of ∆H. Therefore, gentisic acid had more favourable, stabilizing interactions with phospholipids thanβ-sitosterol. Thus, it can be concluded that β-sitosterol is located in the bilayer interior between phospholipids acyl chains, and gentisic acid is incorporated near the outer leaflet of the phospholipid membrane, next to the polar head groups.β-sitosterol and gentisic acid loaded DPPC liposomal particles have a potential to be used in food and pharmaceutical products, due to the important individual and possible synergistic beneficial health properties ofβ-sitosterol and gentisic acid.
PB  - University of East Sarajevo, Faculty of Technology
T2  - Journal of Engineering & Processing Management
T1  - β‐sitosterol and gentisic acid loaded1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphocholine liposomal particles
EP  - 88
IS  - 2
SP  - 81
VL  - 11
DO  - 10.7251/JEPM1902081A
ER  - 

@article{
author = "Jovanović, Aleksandra A. and Balanč, Bojana D. and Ota, Ajda and Đorđević, Verica B. and Šavikin, Katarina P. and Nedović, Viktor A. and Poklar Ulrih, Nataša",
year = "2019",
abstract = "The aim of the present study was the examination of the impact of β-sitosterol and gentisic acid on the characteristics of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine(DPPC) liposomal particles: (a) bilayer permeability (fluorescence spectroscopy),(b) particle size, polydispersity index (PDI) and zeta potential (photon correlation spectroscopy) and (c) thermal properties (differential scanning calorimetry).β-sitosterol induced the increase of liposomal bilayer rigidity, due to rearranging of the phospholipid chains, while gentisic acid enhanced the membrane fluidity, due to the reduced orderliness and the increase of phospholipid dynamics. The inclusion of β-sitosterol in liposomes caused a significant increase in particle diameter and PDI, while the encapsulation of gentisic acid did not have influence on particle size distribution. Apart from that, the presence ofβ-sitosterol resulted in the significant zeta potential increase, and thus a better stability of liposomal spheres(in the absence and in the presence of gentisic acid). β-sitosterol decreased main transition temperature (Tm) and phase transition enthalpy (∆H), and caused the disappearance of the pre-transition peak as well, whereas the presence of gentisic acid produced a slight decrease in Tm and increase of ∆H. Therefore, gentisic acid had more favourable, stabilizing interactions with phospholipids thanβ-sitosterol. Thus, it can be concluded that β-sitosterol is located in the bilayer interior between phospholipids acyl chains, and gentisic acid is incorporated near the outer leaflet of the phospholipid membrane, next to the polar head groups.β-sitosterol and gentisic acid loaded DPPC liposomal particles have a potential to be used in food and pharmaceutical products, due to the important individual and possible synergistic beneficial health properties ofβ-sitosterol and gentisic acid.",
publisher = "University of East Sarajevo, Faculty of Technology",
journal = "Journal of Engineering & Processing Management",
title = "β‐sitosterol and gentisic acid loaded1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphocholine liposomal particles",
pages = "88-81",
number = "2",
volume = "11",
doi = "10.7251/JEPM1902081A"
}

Jovanović, A. A., Balanč, B. D., Ota, A., Đorđević, V. B., Šavikin, K. P., Nedović, V. A.,& Poklar Ulrih, N.. (2019). β‐sitosterol and gentisic acid loaded1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphocholine liposomal particles. in Journal of Engineering & Processing Management
University of East Sarajevo, Faculty of Technology., 11(2), 81-88.
https://doi.org/10.7251/JEPM1902081A

Jovanović AA, Balanč BD, Ota A, Đorđević VB, Šavikin KP, Nedović VA, Poklar Ulrih N. β‐sitosterol and gentisic acid loaded1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphocholine liposomal particles. in Journal of Engineering & Processing Management. 2019;11(2):81-88.
doi:10.7251/JEPM1902081A .

Jovanović, Aleksandra A., Balanč, Bojana D., Ota, Ajda, Đorđević, Verica B., Šavikin, Katarina P., Nedović, Viktor A., Poklar Ulrih, Nataša, "β‐sitosterol and gentisic acid loaded1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphocholine liposomal particles" in Journal of Engineering & Processing Management, 11, no. 2 (2019):81-88,
https://doi.org/10.7251/JEPM1902081A . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Balanč, Bojana
AU  - Đorđević, Verica
AU  - Ota, Ajda
AU  - Skrt, Mihaela
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
AU  - Nedović, Viktor
AU  - Poklar-Ulrih, Nataša
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4088
AB  - Multifunctional liposomes incorporating beta-sitosterol were developed for delivery of gentisic acid (GA). The interactions of both compounds with phospholipid bilayer were interpreted viaeffects of different beta-sitosterol content (0, 20 and 50 mol %) and different gentisic acid to lipid ratio (n(GA)/n(lip) from 10(-5) to 1) on membrane fluidity and thermotropic properties. Multilamellar vesicles of phosphatidylcholines (with size range between 1350 and 1900 nm) effectively encapsulated GA (54%) when n(GA)/n(lip) was higher than 0.01. Suppression of lipid peroxidation was directly related to concentration of GA. The resistance to diffusion of gentisic acid from liposomes increased for (similar to)50% in samples incorporating 50 mol % beta-sitosterol compared to sterol-free liposomes. Finally, simulated in vitro gastrointestinal conditions showed that the release was mainly affected by low pH of simulated gastric fluid and the presence of cholates in simulated intestinal fluid, rather than by enzymes activity.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol
VL  - 183
DO  - 10.1016/j.colsurfb.2019.110422
ER  - 

@article{
author = "Jovanović, Aleksandra and Balanč, Bojana and Đorđević, Verica and Ota, Ajda and Skrt, Mihaela and Šavikin, Katarina and Bugarski, Branko and Nedović, Viktor and Poklar-Ulrih, Nataša",
year = "2019",
abstract = "Multifunctional liposomes incorporating beta-sitosterol were developed for delivery of gentisic acid (GA). The interactions of both compounds with phospholipid bilayer were interpreted viaeffects of different beta-sitosterol content (0, 20 and 50 mol %) and different gentisic acid to lipid ratio (n(GA)/n(lip) from 10(-5) to 1) on membrane fluidity and thermotropic properties. Multilamellar vesicles of phosphatidylcholines (with size range between 1350 and 1900 nm) effectively encapsulated GA (54%) when n(GA)/n(lip) was higher than 0.01. Suppression of lipid peroxidation was directly related to concentration of GA. The resistance to diffusion of gentisic acid from liposomes increased for (similar to)50% in samples incorporating 50 mol % beta-sitosterol compared to sterol-free liposomes. Finally, simulated in vitro gastrointestinal conditions showed that the release was mainly affected by low pH of simulated gastric fluid and the presence of cholates in simulated intestinal fluid, rather than by enzymes activity.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol",
volume = "183",
doi = "10.1016/j.colsurfb.2019.110422"
}

Jovanović, A., Balanč, B., Đorđević, V., Ota, A., Skrt, M., Šavikin, K., Bugarski, B., Nedović, V.,& Poklar-Ulrih, N.. (2019). Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 183.
https://doi.org/10.1016/j.colsurfb.2019.110422

Jovanović A, Balanč B, Đorđević V, Ota A, Skrt M, Šavikin K, Bugarski B, Nedović V, Poklar-Ulrih N. Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol. in Colloids and Surfaces B-Biointerfaces. 2019;183.
doi:10.1016/j.colsurfb.2019.110422 .

Jovanović, Aleksandra, Balanč, Bojana, Đorđević, Verica, Ota, Ajda, Skrt, Mihaela, Šavikin, Katarina, Bugarski, Branko, Nedović, Viktor, Poklar-Ulrih, Nataša, "Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol" in Colloids and Surfaces B-Biointerfaces, 183 (2019),
https://doi.org/10.1016/j.colsurfb.2019.110422 . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Balanč, Bojana
AU  - Ota, Ajda
AU  - Grabnar, Pegi Ahlin
AU  - Đorđević, Verica
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
AU  - Nedović, Viktor
AU  - Poklar-Ulrih, Nataša
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3837
AB  - The influence of different phospholipid types (pure phospholipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, POPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC, and one commercial phospholipid mixture, Lipoid H100), sterol types (cholesterol vs. -sitosterol), and various sterol concentrations (5-50mol%) on liposomal membrane fluidity, thermotropic properties, liposome size, zeta potential, and lipid oxidation kinetics using fluorescent lipid probe BODIPY 581/591 C-11 (4,4-difluoro-5-[4-phenyl-1,3-butadienyl]-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid) are investigated. DPPC bilayer is more rigid than POPC and phospholipids mixture membranes. Pure DPPC gives the smallest liposomes, while liposomes of Lipoid H100 have the largest diameter. Both sterols reduce membrane fluidity of all liposomes, increase absolute zeta potential, cause significant changes in particle size, and decrease phase transition temperature (T-m) and enthalpy of DPPC. POPC/-sitosterol liposomes exhibit the most significant lipid oxidation of the lipophilic probe. Along with beneficial effects of phytosterols on human health, better membrane fluidity, more favorable and stabilizing interactions with phospholipids, smaller vesicle size, and enhanced physical stability in comparison to cholesterol are some of the encouraging results for the use of -sitosterol in liposome formulations for potential application in foods, pharmaceutics, and cosmetics.Practical Applications: Adjusting the composition of liposomal membrane (lipid type, sterol type, and concentration) can be used as a tool to control membrane fluidity, permeability, and thermotropic properties, and thus predict release properties, physical, thermal, and oxidative stability. A commercial phospholipid mixture of different natural phospholipids with impurities creates less uniform liposomal membrane that is characterized by higher fluidity in comparison to DPPC. The type of phospholipid has huge influence on MLVs size. -sitosterol, which is a phytosterol with beneficial effects on human health can be used as a replacement for cholesterol in liposomal formulations, but with the following in mind: -sitosterol reduces fluidity of the phospholipid bilayer to a lesser extent than cholesterol, -sitosterol gives smaller MLVs than cholesterol, DPPC/-sitosterol SUVs are bigger than 100nm in diameter (relevant for intravenous administration), MLVs with 30mol% of -sitosterol can be considered as physically stable (unlike those with cholesterol), irrespective to the phospholipid type.
PB  - Wiley, Hoboken
T2  - European Journal of Lipid Science and Technology
T1  - Comparative Effects of Cholesterol and β-Sitosterol on the Liposome Membrane Characteristics
IS  - 9
VL  - 120
DO  - 10.1002/ejlt.201800039
ER  - 

@article{
author = "Jovanović, Aleksandra and Balanč, Bojana and Ota, Ajda and Grabnar, Pegi Ahlin and Đorđević, Verica and Šavikin, Katarina and Bugarski, Branko and Nedović, Viktor and Poklar-Ulrih, Nataša",
year = "2018",
abstract = "The influence of different phospholipid types (pure phospholipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, POPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC, and one commercial phospholipid mixture, Lipoid H100), sterol types (cholesterol vs. -sitosterol), and various sterol concentrations (5-50mol%) on liposomal membrane fluidity, thermotropic properties, liposome size, zeta potential, and lipid oxidation kinetics using fluorescent lipid probe BODIPY 581/591 C-11 (4,4-difluoro-5-[4-phenyl-1,3-butadienyl]-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid) are investigated. DPPC bilayer is more rigid than POPC and phospholipids mixture membranes. Pure DPPC gives the smallest liposomes, while liposomes of Lipoid H100 have the largest diameter. Both sterols reduce membrane fluidity of all liposomes, increase absolute zeta potential, cause significant changes in particle size, and decrease phase transition temperature (T-m) and enthalpy of DPPC. POPC/-sitosterol liposomes exhibit the most significant lipid oxidation of the lipophilic probe. Along with beneficial effects of phytosterols on human health, better membrane fluidity, more favorable and stabilizing interactions with phospholipids, smaller vesicle size, and enhanced physical stability in comparison to cholesterol are some of the encouraging results for the use of -sitosterol in liposome formulations for potential application in foods, pharmaceutics, and cosmetics.Practical Applications: Adjusting the composition of liposomal membrane (lipid type, sterol type, and concentration) can be used as a tool to control membrane fluidity, permeability, and thermotropic properties, and thus predict release properties, physical, thermal, and oxidative stability. A commercial phospholipid mixture of different natural phospholipids with impurities creates less uniform liposomal membrane that is characterized by higher fluidity in comparison to DPPC. The type of phospholipid has huge influence on MLVs size. -sitosterol, which is a phytosterol with beneficial effects on human health can be used as a replacement for cholesterol in liposomal formulations, but with the following in mind: -sitosterol reduces fluidity of the phospholipid bilayer to a lesser extent than cholesterol, -sitosterol gives smaller MLVs than cholesterol, DPPC/-sitosterol SUVs are bigger than 100nm in diameter (relevant for intravenous administration), MLVs with 30mol% of -sitosterol can be considered as physically stable (unlike those with cholesterol), irrespective to the phospholipid type.",
publisher = "Wiley, Hoboken",
journal = "European Journal of Lipid Science and Technology",
title = "Comparative Effects of Cholesterol and β-Sitosterol on the Liposome Membrane Characteristics",
number = "9",
volume = "120",
doi = "10.1002/ejlt.201800039"
}

Jovanović, A., Balanč, B., Ota, A., Grabnar, P. A., Đorđević, V., Šavikin, K., Bugarski, B., Nedović, V.,& Poklar-Ulrih, N.. (2018). Comparative Effects of Cholesterol and β-Sitosterol on the Liposome Membrane Characteristics. in European Journal of Lipid Science and Technology
Wiley, Hoboken., 120(9).
https://doi.org/10.1002/ejlt.201800039

Jovanović A, Balanč B, Ota A, Grabnar PA, Đorđević V, Šavikin K, Bugarski B, Nedović V, Poklar-Ulrih N. Comparative Effects of Cholesterol and β-Sitosterol on the Liposome Membrane Characteristics. in European Journal of Lipid Science and Technology. 2018;120(9).
doi:10.1002/ejlt.201800039 .

Jovanović, Aleksandra, Balanč, Bojana, Ota, Ajda, Grabnar, Pegi Ahlin, Đorđević, Verica, Šavikin, Katarina, Bugarski, Branko, Nedović, Viktor, Poklar-Ulrih, Nataša, "Comparative Effects of Cholesterol and β-Sitosterol on the Liposome Membrane Characteristics" in European Journal of Lipid Science and Technology, 120, no. 9 (2018),
https://doi.org/10.1002/ejlt.201800039 . .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana
AU  - Pravilović, Radoslava
AU  - Ota, Ajda
AU  - Poklar-Ulrih, Nataša
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6447
AB  - Cilj ove studije bilo je ispitivanje uticaja različitih sadržaja holesterola (0-
50 mol %) na fluidnost membrana, veličinu čestica, polidisperzni indeks
(PDI) i zeta potencijal lipozoma, pripremljenih metodom tankog filma i
upotrebom fosfatidilholina soje, Phospholipon 90 (Ph). Merenjem
polarizacije (p) i anizotropije (r), u temperaturnom intervalu od 20 do 60˚C,
upotrebom fluorofore 1,6-difenil-1,3,5-heksatrien (DPH), pokazano je da
povećanjem koncentracije holesterola u lipozomima dolazi do smanjenja
fluidnosti i povećanja rigidnosti membrane lipozoma. Foton korelaciona
spektroskopija je kod malih unilamelarnih lipozoma (SUV) pokazala
kontinuirano povećanje veličine čestica sa porastom koncentracije
holesterola, dok kod multilamelarnih lipozoma (MLV) trend kontinuiranog
rasta čestica nije primećen. U oba slučaja, statistički značajno veće čestice
su dobijene sa najvišom koncentracijom holesterola (50 mol %). Prema
dobijenim vrednostima PDI, samo SUV bez holesterola i sa 5, 30 i 40 mol %
holesterola, poseduju usku raspodelu čestica po veličini (PDI manji od 0,2).
Vrednosti zeta potencijala, kao važnog parametra stabilnosti sistema, bile su
negativne u svim uzorcima, dok su najviše apsolutne vrednosti dostignute sa
50 mol % holesterola. Optimalna koncentracija holesterola za dobijanje
lipozoma odgovarajuće stabilnosti i fluidnosti membrana jeste 50 mol %.
AB  - The aim of this study was examination of the influence of various cholesterol
content (0-50 mol %) on membrane fluidity, vesicle size, polydispersity index
(PDI) and zeta potential of liposomes prepared using the thin film method
and phosphatidylcholine from soybean, Phospholipon 90 (Ph). Polarization
(p) and anisotropy (r) measurements, within the temperature range of 20 to
60˚C, using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH), have
shown that increasing the cholesterol content in the liposomes decreased the
fluidity and enhanced the rigidity of liposomal membranes. The photon
correlation spectroscopy data have shown that the size of small unilamellar
vesicles (SUV) increased continuously with the increase in cholesterol
concentration, while multilamellar vesicles (MLV) did not show similar
trend. In both cases, significantly larger vesicles were obtained using the
highest content of cholesterol (50 mol %). According to the PDI values, only
SUV without cholesterol and with 5, 30 and 40 mol % of cholesterol exhibit
narrow size distribution (PDI < 0,2). Furthermore, values of zeta potential,
as an important parameter of system stability, were negative in all samples,
while the highest absolute values were achieved with 50 mol % of cholesterol. The optimal concentration of cholesterol to obtain stabile
liposomes and an appropriate fluidity of the membrane has been 50 mol %.
PB  - Faculty of Technology, Zvornik
C3  - V International Congress: „Engineering, Environment and Materials in Processing Industry“
T1  - Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal
EP  - 1514
SP  - 1502
DO  - 10.7251/EEMSR15011502J
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Balanč, Bojana and Pravilović, Radoslava and Ota, Ajda and Poklar-Ulrih, Nataša and Nedović, Viktor and Bugarski, Branko",
year = "2017",
abstract = "Cilj ove studije bilo je ispitivanje uticaja različitih sadržaja holesterola (0-
50 mol %) na fluidnost membrana, veličinu čestica, polidisperzni indeks
(PDI) i zeta potencijal lipozoma, pripremljenih metodom tankog filma i
upotrebom fosfatidilholina soje, Phospholipon 90 (Ph). Merenjem
polarizacije (p) i anizotropije (r), u temperaturnom intervalu od 20 do 60˚C,
upotrebom fluorofore 1,6-difenil-1,3,5-heksatrien (DPH), pokazano je da
povećanjem koncentracije holesterola u lipozomima dolazi do smanjenja
fluidnosti i povećanja rigidnosti membrane lipozoma. Foton korelaciona
spektroskopija je kod malih unilamelarnih lipozoma (SUV) pokazala
kontinuirano povećanje veličine čestica sa porastom koncentracije
holesterola, dok kod multilamelarnih lipozoma (MLV) trend kontinuiranog
rasta čestica nije primećen. U oba slučaja, statistički značajno veće čestice
su dobijene sa najvišom koncentracijom holesterola (50 mol %). Prema
dobijenim vrednostima PDI, samo SUV bez holesterola i sa 5, 30 i 40 mol %
holesterola, poseduju usku raspodelu čestica po veličini (PDI manji od 0,2).
Vrednosti zeta potencijala, kao važnog parametra stabilnosti sistema, bile su
negativne u svim uzorcima, dok su najviše apsolutne vrednosti dostignute sa
50 mol % holesterola. Optimalna koncentracija holesterola za dobijanje
lipozoma odgovarajuće stabilnosti i fluidnosti membrana jeste 50 mol %., The aim of this study was examination of the influence of various cholesterol
content (0-50 mol %) on membrane fluidity, vesicle size, polydispersity index
(PDI) and zeta potential of liposomes prepared using the thin film method
and phosphatidylcholine from soybean, Phospholipon 90 (Ph). Polarization
(p) and anisotropy (r) measurements, within the temperature range of 20 to
60˚C, using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH), have
shown that increasing the cholesterol content in the liposomes decreased the
fluidity and enhanced the rigidity of liposomal membranes. The photon
correlation spectroscopy data have shown that the size of small unilamellar
vesicles (SUV) increased continuously with the increase in cholesterol
concentration, while multilamellar vesicles (MLV) did not show similar
trend. In both cases, significantly larger vesicles were obtained using the
highest content of cholesterol (50 mol %). According to the PDI values, only
SUV without cholesterol and with 5, 30 and 40 mol % of cholesterol exhibit
narrow size distribution (PDI < 0,2). Furthermore, values of zeta potential,
as an important parameter of system stability, were negative in all samples,
while the highest absolute values were achieved with 50 mol % of cholesterol. The optimal concentration of cholesterol to obtain stabile
liposomes and an appropriate fluidity of the membrane has been 50 mol %.",
publisher = "Faculty of Technology, Zvornik",
journal = "V International Congress: „Engineering, Environment and Materials in Processing Industry“",
title = "Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal",
pages = "1514-1502",
doi = "10.7251/EEMSR15011502J"
}

Jovanović, A. A., Balanč, B., Pravilović, R., Ota, A., Poklar-Ulrih, N., Nedović, V.,& Bugarski, B.. (2017). Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal. in V International Congress: „Engineering, Environment and Materials in Processing Industry“
Faculty of Technology, Zvornik., 1502-1514.
https://doi.org/10.7251/EEMSR15011502J

Jovanović AA, Balanč B, Pravilović R, Ota A, Poklar-Ulrih N, Nedović V, Bugarski B. Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal. in V International Congress: „Engineering, Environment and Materials in Processing Industry“. 2017;:1502-1514.
doi:10.7251/EEMSR15011502J .

Jovanović, Aleksandra A., Balanč, Bojana, Pravilović, Radoslava, Ota, Ajda, Poklar-Ulrih, Nataša, Nedović, Viktor, Bugarski, Branko, "Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal" in V International Congress: „Engineering, Environment and Materials in Processing Industry“ (2017):1502-1514,
https://doi.org/10.7251/EEMSR15011502J . .

TY  - JOUR
AU  - Balanč, Bojana
AU  - Ota, Ajda
AU  - Đorđević, Verica
AU  - Sentjurc, Marjeta
AU  - Nedović, Viktor
AU  - Bugarski, Branko
AU  - Poklar-Ulrih, Nataša
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3075
AB  - The influence of resveratrol encapsulated into liposomes (prepared with a commercial lipid mixture of phospholipids, phospolipon 90NG, using the thin film and proliposome methods) on the structural properties of the liposome membrane was investigated by electron paramagnetic resonance (EPR) spectroscopy, fluorescence spectroscopy, and differential scanning calorimetry. Two fluorophores and two spin probes were used to monitor the characteristics of membranes made from a commercial mixture of phosphatidylcholine. Resveratrol was positioned rather in the inner part of the liposome membranes causing reduction in membrane fluidity. Moreover, resveratrol induced a concentration-dependent decrease in the gel-to-liquid crystalline phase transition temperature (from 41.3 to 38.3 degrees C, for a saturated 1,2-dipalmitoyl-sn-glycero-3-phospatidylcholine bilayer). The antioxidant activity of resveratrol was confirmed by its 95% inhibition of lipid peroxidation, compared to liposomes without resveratrol exposed to the same conditions. Similarly, EPR spectroscopy spin trapping showed an 87% reduction in the spectra intensity of the hydroxyethyl radical, which indicates the efficiency of resveratrol for inhibition of OH radical production. Practical applications: Resveratrol is in the limelight all over the world as a health-beneficial compound widely investigated as natural antioxidant suitable for prevention of human cardiovascular diseases and inhibition of low-density lipoproteins oxidation. Public interest in prevention of diseases through enriched staple foods grows each day. Nonetheless, addition of antioxidants to aqueous-based food can be limited due to their troublesome characteristics. Liposomes are nanocarriers that may be used to overcome the disadvantages and they can be used for food applications. Liposomes could be prepared using only natural components and therefore the new formulations could be quickly implemented. Still, the application of liposomes in food systems is not widespread due to time consuming manufacturing processes and high costs. The presented results provide marks and suggestions to food manufacturers and scientists on how to make broader use of resveratrol-loaded liposomes which add value and improve the quality of existing food products. The influence of resveratrol encapsulated into liposomes (prepared using the thin film and proliposome methods) on the structural properties of the liposome membrane was investigated by electron paramagnetic resonance spectroscopy, fluorescence spectroscopy, and differential scanning calorimetry. The antioxidant activity of resveratrol was confirmed by its inhibition of lipid peroxidation and by spin trapping.
PB  - Wiley, Hoboken
T2  - European Journal of Lipid Science and Technology
T1  - Resveratrol-loaded liposomes: Interaction of resveratrol with phospholipids
EP  - 1626
IS  - 10
SP  - 1615
VL  - 117
DO  - 10.1002/ejlt.201400481
ER  - 

@article{
author = "Balanč, Bojana and Ota, Ajda and Đorđević, Verica and Sentjurc, Marjeta and Nedović, Viktor and Bugarski, Branko and Poklar-Ulrih, Nataša",
year = "2015",
abstract = "The influence of resveratrol encapsulated into liposomes (prepared with a commercial lipid mixture of phospholipids, phospolipon 90NG, using the thin film and proliposome methods) on the structural properties of the liposome membrane was investigated by electron paramagnetic resonance (EPR) spectroscopy, fluorescence spectroscopy, and differential scanning calorimetry. Two fluorophores and two spin probes were used to monitor the characteristics of membranes made from a commercial mixture of phosphatidylcholine. Resveratrol was positioned rather in the inner part of the liposome membranes causing reduction in membrane fluidity. Moreover, resveratrol induced a concentration-dependent decrease in the gel-to-liquid crystalline phase transition temperature (from 41.3 to 38.3 degrees C, for a saturated 1,2-dipalmitoyl-sn-glycero-3-phospatidylcholine bilayer). The antioxidant activity of resveratrol was confirmed by its 95% inhibition of lipid peroxidation, compared to liposomes without resveratrol exposed to the same conditions. Similarly, EPR spectroscopy spin trapping showed an 87% reduction in the spectra intensity of the hydroxyethyl radical, which indicates the efficiency of resveratrol for inhibition of OH radical production. Practical applications: Resveratrol is in the limelight all over the world as a health-beneficial compound widely investigated as natural antioxidant suitable for prevention of human cardiovascular diseases and inhibition of low-density lipoproteins oxidation. Public interest in prevention of diseases through enriched staple foods grows each day. Nonetheless, addition of antioxidants to aqueous-based food can be limited due to their troublesome characteristics. Liposomes are nanocarriers that may be used to overcome the disadvantages and they can be used for food applications. Liposomes could be prepared using only natural components and therefore the new formulations could be quickly implemented. Still, the application of liposomes in food systems is not widespread due to time consuming manufacturing processes and high costs. The presented results provide marks and suggestions to food manufacturers and scientists on how to make broader use of resveratrol-loaded liposomes which add value and improve the quality of existing food products. The influence of resveratrol encapsulated into liposomes (prepared using the thin film and proliposome methods) on the structural properties of the liposome membrane was investigated by electron paramagnetic resonance spectroscopy, fluorescence spectroscopy, and differential scanning calorimetry. The antioxidant activity of resveratrol was confirmed by its inhibition of lipid peroxidation and by spin trapping.",
publisher = "Wiley, Hoboken",
journal = "European Journal of Lipid Science and Technology",
title = "Resveratrol-loaded liposomes: Interaction of resveratrol with phospholipids",
pages = "1626-1615",
number = "10",
volume = "117",
doi = "10.1002/ejlt.201400481"
}

Balanč, B., Ota, A., Đorđević, V., Sentjurc, M., Nedović, V., Bugarski, B.,& Poklar-Ulrih, N.. (2015). Resveratrol-loaded liposomes: Interaction of resveratrol with phospholipids. in European Journal of Lipid Science and Technology
Wiley, Hoboken., 117(10), 1615-1626.
https://doi.org/10.1002/ejlt.201400481

Balanč B, Ota A, Đorđević V, Sentjurc M, Nedović V, Bugarski B, Poklar-Ulrih N. Resveratrol-loaded liposomes: Interaction of resveratrol with phospholipids. in European Journal of Lipid Science and Technology. 2015;117(10):1615-1626.
doi:10.1002/ejlt.201400481 .

Balanč, Bojana, Ota, Ajda, Đorđević, Verica, Sentjurc, Marjeta, Nedović, Viktor, Bugarski, Branko, Poklar-Ulrih, Nataša, "Resveratrol-loaded liposomes: Interaction of resveratrol with phospholipids" in European Journal of Lipid Science and Technology, 117, no. 10 (2015):1615-1626,
https://doi.org/10.1002/ejlt.201400481 . .