TY  - JOUR
AU  - Batinić, Petar M.
AU  - Đorđević, Verica B.
AU  - Obradović, Nataša S.
AU  - Krstić, Aleksandar D.
AU  - Stevanović, Sanja I.
AU  - Balanč, Bojana D.
AU  - Marković, Smilja B.
AU  - Pjanović, Rada V.
AU  - Mijin, Dušan Ž.
AU  - Bugarski, Branko M.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6358
AB  - Liposome-encapsulated folic acid is incorporated into the films made from sodium carboxymethyl cellulose (CMC) (2 mas%) and a mixture of CMC and solagum (9:1 w/w) using the film-forming cast solution method. Histidine is used to increase solubility for folic acid in liposomes (1–5 mg mL−1), and propylene glycol is used as a film plasticizer (2.6 mas%). The obtained films (50–60 µm tick) containing 3.12–20.19 mg of folic acid per gram of film are envisaged to be used as patches for transdermal delivery of folic acid. Therefore, some physical, mechanical, release and structural attributes of the films are scrutinized. Folic acid gives yellow color to the films and contributes to stronger chemical bonds which result in improved strength of the film. Liposomes prolong the release of folic acid from films to 24 h without adverse effects on mechanical properties of the films, but degrade homogeneity of the films, which can be ascribed to its agglomeration within the film matrix as revealed by atomic force microscopy. According to the release at pH 5.5, the film formulation based on a blend of CMC and solagum containing 3 mg mL−1 liposome-encapsulated folic acid is recommended. Practical Application: Folic acid is effective in reducing oxidative stress levels in the skin and neutralizing the harmful free radicals and is also essential for various metabolic reactions in the body. However, the limited solubility of folic acid linked with its poor absorption in an organism, low storage stability, short half-life upon oral consumption, specific food preferences of some people, extensive liver metabolism, and pregnancy-induced vomiting point to a large potential in transdermal usage of folic acid. This has motivated us to design new multicomponent polymer-lipid systems as an alternative solution to overcome some of these drawbacks. The results obtained for these multicomponent films pointed to their potential for prolonged release of folic acid to 24 h, which can also be useful for scientists interested in encapsulating similar poorly soluble compounds in CMC patches. The finding can be also valuable information for pharmaceutical manufacturers and scientists worldwide.
PB  - John Wiley and Sons Inc.
T2  - European Journal of Lipid Science and Technology
T1  - Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid
SP  - 2200169
DO  - 10.1002/ejlt.202200169
ER  - 

@article{
author = "Batinić, Petar M. and Đorđević, Verica B. and Obradović, Nataša S. and Krstić, Aleksandar D. and Stevanović, Sanja I. and Balanč, Bojana D. and Marković, Smilja B. and Pjanović, Rada V. and Mijin, Dušan Ž. and Bugarski, Branko M.",
year = "2023",
abstract = "Liposome-encapsulated folic acid is incorporated into the films made from sodium carboxymethyl cellulose (CMC) (2 mas%) and a mixture of CMC and solagum (9:1 w/w) using the film-forming cast solution method. Histidine is used to increase solubility for folic acid in liposomes (1–5 mg mL−1), and propylene glycol is used as a film plasticizer (2.6 mas%). The obtained films (50–60 µm tick) containing 3.12–20.19 mg of folic acid per gram of film are envisaged to be used as patches for transdermal delivery of folic acid. Therefore, some physical, mechanical, release and structural attributes of the films are scrutinized. Folic acid gives yellow color to the films and contributes to stronger chemical bonds which result in improved strength of the film. Liposomes prolong the release of folic acid from films to 24 h without adverse effects on mechanical properties of the films, but degrade homogeneity of the films, which can be ascribed to its agglomeration within the film matrix as revealed by atomic force microscopy. According to the release at pH 5.5, the film formulation based on a blend of CMC and solagum containing 3 mg mL−1 liposome-encapsulated folic acid is recommended. Practical Application: Folic acid is effective in reducing oxidative stress levels in the skin and neutralizing the harmful free radicals and is also essential for various metabolic reactions in the body. However, the limited solubility of folic acid linked with its poor absorption in an organism, low storage stability, short half-life upon oral consumption, specific food preferences of some people, extensive liver metabolism, and pregnancy-induced vomiting point to a large potential in transdermal usage of folic acid. This has motivated us to design new multicomponent polymer-lipid systems as an alternative solution to overcome some of these drawbacks. The results obtained for these multicomponent films pointed to their potential for prolonged release of folic acid to 24 h, which can also be useful for scientists interested in encapsulating similar poorly soluble compounds in CMC patches. The finding can be also valuable information for pharmaceutical manufacturers and scientists worldwide.",
publisher = "John Wiley and Sons Inc.",
journal = "European Journal of Lipid Science and Technology",
title = "Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid",
pages = "2200169",
doi = "10.1002/ejlt.202200169"
}

Batinić, P. M., Đorđević, V. B., Obradović, N. S., Krstić, A. D., Stevanović, S. I., Balanč, B. D., Marković, S. B., Pjanović, R. V., Mijin, D. Ž.,& Bugarski, B. M.. (2023). Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid. in European Journal of Lipid Science and Technology
John Wiley and Sons Inc.., 2200169.
https://doi.org/10.1002/ejlt.202200169

Batinić PM, Đorđević VB, Obradović NS, Krstić AD, Stevanović SI, Balanč BD, Marković SB, Pjanović RV, Mijin DŽ, Bugarski BM. Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid. in European Journal of Lipid Science and Technology. 2023;:2200169.
doi:10.1002/ejlt.202200169 .

Batinić, Petar M., Đorđević, Verica B., Obradović, Nataša S., Krstić, Aleksandar D., Stevanović, Sanja I., Balanč, Bojana D., Marković, Smilja B., Pjanović, Rada V., Mijin, Dušan Ž., Bugarski, Branko M., "Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid" in European Journal of Lipid Science and Technology (2023):2200169,
https://doi.org/10.1002/ejlt.202200169 . .

TY  - JOUR
AU  - Marković, Maja D.
AU  - Svetozarević, Milica M.
AU  - Panić, Vesna V.
AU  - Savić, Sanja I.
AU  - Mašulović, Aleksandra D.
AU  - Spasojević, Pavle M.
AU  - Pjanović, Rada V.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5860
AB  - This study reports successful free radical synthesis of pH-sensitive hydrogels based on poly(methacrylic acid) (PMAA) by using new green initiation system based on vitamin C and hydrogen peroxide (VC/H2O2). The application of proposed initiation system provides many advantages, above all cost effective and eco-friendly synthesis which can be carried out under ambient conditions. The obtained PMAA hydrogels are analyzed by various technics: Differential Scanning Calorimetry, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy and by using single compression tests. In line with the intended application, PMAA hydrogels are further investigated in terms of their potential to be used for encapsulation and controlled release of active substances such as antioxidant phenolic compounds. To keep it green, the total phenolic compounds (TPC) were obtained from orange peels waste by applying ultrasonic-assisted extraction and deep eutectic solvent (DES) based on glycerol:urea:water. The TPC were successfully encapsulated into the PMAA hydrogels which were previously estimated to have the optimal mechanical and swelling properties with respect to the final application. The swelling behavior of the PMAA hydrogels and controlled release of the TPC were tested as a function of the various synthesis parameters in several media with different pH values. It was shown that TPC can be released in control manner in medium which simulates the environment in human intestines, finally resulting in enhanced bioavailability of TPC, reduced side effects and improved therapeutic effects.
PB  - Elsevier B.V.
T2  - Chemical Engineering Journal
T1  - Novel eco-friendly initiation system based on vitamin C for energy efficient synthesis of PMAA hydrogel used for delivery of phenolic compounds
SP  - 141580
VL  - 459
DO  - 10.1016/j.cej.2023.141580
ER  - 

@article{
author = "Marković, Maja D. and Svetozarević, Milica M. and Panić, Vesna V. and Savić, Sanja I. and Mašulović, Aleksandra D. and Spasojević, Pavle M. and Pjanović, Rada V.",
year = "2023",
abstract = "This study reports successful free radical synthesis of pH-sensitive hydrogels based on poly(methacrylic acid) (PMAA) by using new green initiation system based on vitamin C and hydrogen peroxide (VC/H2O2). The application of proposed initiation system provides many advantages, above all cost effective and eco-friendly synthesis which can be carried out under ambient conditions. The obtained PMAA hydrogels are analyzed by various technics: Differential Scanning Calorimetry, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy and by using single compression tests. In line with the intended application, PMAA hydrogels are further investigated in terms of their potential to be used for encapsulation and controlled release of active substances such as antioxidant phenolic compounds. To keep it green, the total phenolic compounds (TPC) were obtained from orange peels waste by applying ultrasonic-assisted extraction and deep eutectic solvent (DES) based on glycerol:urea:water. The TPC were successfully encapsulated into the PMAA hydrogels which were previously estimated to have the optimal mechanical and swelling properties with respect to the final application. The swelling behavior of the PMAA hydrogels and controlled release of the TPC were tested as a function of the various synthesis parameters in several media with different pH values. It was shown that TPC can be released in control manner in medium which simulates the environment in human intestines, finally resulting in enhanced bioavailability of TPC, reduced side effects and improved therapeutic effects.",
publisher = "Elsevier B.V.",
journal = "Chemical Engineering Journal",
title = "Novel eco-friendly initiation system based on vitamin C for energy efficient synthesis of PMAA hydrogel used for delivery of phenolic compounds",
pages = "141580",
volume = "459",
doi = "10.1016/j.cej.2023.141580"
}

Marković, M. D., Svetozarević, M. M., Panić, V. V., Savić, S. I., Mašulović, A. D., Spasojević, P. M.,& Pjanović, R. V.. (2023). Novel eco-friendly initiation system based on vitamin C for energy efficient synthesis of PMAA hydrogel used for delivery of phenolic compounds. in Chemical Engineering Journal
Elsevier B.V.., 459, 141580.
https://doi.org/10.1016/j.cej.2023.141580

Marković MD, Svetozarević MM, Panić VV, Savić SI, Mašulović AD, Spasojević PM, Pjanović RV. Novel eco-friendly initiation system based on vitamin C for energy efficient synthesis of PMAA hydrogel used for delivery of phenolic compounds. in Chemical Engineering Journal. 2023;459:141580.
doi:10.1016/j.cej.2023.141580 .

Marković, Maja D., Svetozarević, Milica M., Panić, Vesna V., Savić, Sanja I., Mašulović, Aleksandra D., Spasojević, Pavle M., Pjanović, Rada V., "Novel eco-friendly initiation system based on vitamin C for energy efficient synthesis of PMAA hydrogel used for delivery of phenolic compounds" in Chemical Engineering Journal, 459 (2023):141580,
https://doi.org/10.1016/j.cej.2023.141580 . .

TY  - PAT
AU  - Marković, Maja
AU  - Tadić, Julijana
AU  - Šešlija, Sanja
AU  - Mijin, Dušan
AU  - Panić, Vesna
AU  - Spasojević, Pavle
AU  - Pjanović, Rada
AU  - Ugrinović, Vukašin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5950
AB  - Predmetnim pronalaskom postignuta je inkapsulacija slabo vodorastvorne aktivne supstance heterocikličnog azo jedinjenja sa antikancerogenim svojstvima u hidrofilni hidrogel na bazi poli(metakrilne kiseline) modifikovanog amfifilnim kazeinom, ciljana dostava inkapsuliranog azo derivata i njegovo kontrolisano i produženo otpuštanje (i do 72h) bez naglog inicijalnog oslobadanja iz hidrofilnog hidrogela. Takođe, predmetnim pronalaskom su dobijeni nosači koji bi imali potencijalnu primenu u tretmanu hronične mijeloidne leukemije (HML), čijom bi se primenom smanjio broj potrebnih terapeutskih doza i redukovali neželjeni efekti (kao što je povraćanje i iritacija gastrointestinalnog trakta) koji se najčešće javljaju kod primene lekova za terapiju HML.
PB  - Zavod za intelektualnu svojinu, Beograd, Srbija
T2  - http://pub.zis.gov.rs/rs-pubserver/document?iDocId=103007&iepatch=.pdf
T1  - SISTEM NA BAZI POLI (METAKRILNE KISELINE) I KAZEINA ZA KONTROLISANO OTPUŠTANJE HETEROCIKLIČNOG AZO JEDINJENJA SA POTENCIJALNOM PRIMENOM U TRETMANU MALIGNOG OBOLJENJA BELIH KRVNIH ĆELIJA
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5950
ER  - 

@misc{
author = "Marković, Maja and Tadić, Julijana and Šešlija, Sanja and Mijin, Dušan and Panić, Vesna and Spasojević, Pavle and Pjanović, Rada and Ugrinović, Vukašin",
year = "2023",
abstract = "Predmetnim pronalaskom postignuta je inkapsulacija slabo vodorastvorne aktivne supstance heterocikličnog azo jedinjenja sa antikancerogenim svojstvima u hidrofilni hidrogel na bazi poli(metakrilne kiseline) modifikovanog amfifilnim kazeinom, ciljana dostava inkapsuliranog azo derivata i njegovo kontrolisano i produženo otpuštanje (i do 72h) bez naglog inicijalnog oslobadanja iz hidrofilnog hidrogela. Takođe, predmetnim pronalaskom su dobijeni nosači koji bi imali potencijalnu primenu u tretmanu hronične mijeloidne leukemije (HML), čijom bi se primenom smanjio broj potrebnih terapeutskih doza i redukovali neželjeni efekti (kao što je povraćanje i iritacija gastrointestinalnog trakta) koji se najčešće javljaju kod primene lekova za terapiju HML.",
publisher = "Zavod za intelektualnu svojinu, Beograd, Srbija",
journal = "http://pub.zis.gov.rs/rs-pubserver/document?iDocId=103007&iepatch=.pdf",
title = "SISTEM NA BAZI POLI (METAKRILNE KISELINE) I KAZEINA ZA KONTROLISANO OTPUŠTANJE HETEROCIKLIČNOG AZO JEDINJENJA SA POTENCIJALNOM PRIMENOM U TRETMANU MALIGNOG OBOLJENJA BELIH KRVNIH ĆELIJA",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5950"
}

Marković, M., Tadić, J., Šešlija, S., Mijin, D., Panić, V., Spasojević, P., Pjanović, R.,& Ugrinović, V.. (2023). SISTEM NA BAZI POLI (METAKRILNE KISELINE) I KAZEINA ZA KONTROLISANO OTPUŠTANJE HETEROCIKLIČNOG AZO JEDINJENJA SA POTENCIJALNOM PRIMENOM U TRETMANU MALIGNOG OBOLJENJA BELIH KRVNIH ĆELIJA. in http://pub.zis.gov.rs/rs-pubserver/document?iDocId=103007&iepatch=.pdf
Zavod za intelektualnu svojinu, Beograd, Srbija..
https://hdl.handle.net/21.15107/rcub_technorep_5950

Marković M, Tadić J, Šešlija S, Mijin D, Panić V, Spasojević P, Pjanović R, Ugrinović V. SISTEM NA BAZI POLI (METAKRILNE KISELINE) I KAZEINA ZA KONTROLISANO OTPUŠTANJE HETEROCIKLIČNOG AZO JEDINJENJA SA POTENCIJALNOM PRIMENOM U TRETMANU MALIGNOG OBOLJENJA BELIH KRVNIH ĆELIJA. in http://pub.zis.gov.rs/rs-pubserver/document?iDocId=103007&iepatch=.pdf. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_5950 .

Marković, Maja, Tadić, Julijana, Šešlija, Sanja, Mijin, Dušan, Panić, Vesna, Spasojević, Pavle, Pjanović, Rada, Ugrinović, Vukašin, "SISTEM NA BAZI POLI (METAKRILNE KISELINE) I KAZEINA ZA KONTROLISANO OTPUŠTANJE HETEROCIKLIČNOG AZO JEDINJENJA SA POTENCIJALNOM PRIMENOM U TRETMANU MALIGNOG OBOLJENJA BELIH KRVNIH ĆELIJA" in http://pub.zis.gov.rs/rs-pubserver/document?iDocId=103007&iepatch=.pdf (2023),
https://hdl.handle.net/21.15107/rcub_technorep_5950 .

TY  - CONF
AU  - Marković, Maja D.
AU  - Svetozarević, Milica M.
AU  - Ugrinović, Vukašin Đ.
AU  - Pjanović, Rada V.
AU  - Obradović, Bojana M.
AU  - Kalagasidis Krušić, Melina T.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6620
AB  - Everyday struggle of humanity with novel diseases and present once, urge researchers to find novel and improve existing therapies to enhance their efficiency and safety. One of the promising approaches to overcome these challenges is controlled release of drugs. Biomaterials based on poly(methacrylic acid) (PMAA) are excellent drug delivery systems because they can control release rate and released amount of drug. Also, due to their pH sensitivity the PMAA hydrogels can release drug at the site of action. Namely, these nontoxic and biocompatible hydrogels swell in the environment with pH value higher than pKa of PMAA (4.6) and release encapsulated drug during the process. In present study, PMAA hydrogels are synthetized under ambient conditions by simple, cost effective and eco-friendly synthesis. Novel initiation system based on hydrogen peroxide, potato peel peroxidase and vitamin C (VC) is used for the first time for free radical polymerization of PMAA hydrogel. In accordance with the principles of circular economy, peroxidase was isolated from potato peel waste by water extraction for 12 h at 4 ℃. Four PMAA hydrogels were prepared by using potato peel peroxidase with various enzyme activity (0.4; 0.8; 1.2 and 1.8 IU), whereas the amounts of H2O2 (30 mL) and VC (10 mg) have been kept constant. The composition of the PMAA hydrogels was confirmed by FTIR analysis, whereas their porous structure was revealed by SEM. The swelling of the PMAA hydrogels was monitored in two media: 0.1M HCl (as simulation of human stomach) and phosphate buffer with pH 7.4 (as simulation of human intestines). In order to encapsulate anti-inflammatory drug – dexamethasone into the PMAA hydrogels, the hydrogels were immersed into the dexamethasone aqueous solution (5 mg/ml) and left to swell to the equilibrium, after which they were dried at room temperature. Dexamethasone release from the PMAA hydrogels was monitored in the same environments as was the PMAA swelling. The results showed that around seven times higher amount of dexamethasone was released in the medium with pH 7.4 than in the medium with pH 1. Present study has showed that the PMAA hydrogels, prepared through green and eco-friendly method, have huge potential for encapsulation and controlled release of anti-inflammatory drugs and therefore, for the treatment of rheumatoid arthritis.
PB  - AO Foundation
C3  - ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical
T1  - Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs
SP  - 572
VL  - 3
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6620
ER  - 

@conference{
author = "Marković, Maja D. and Svetozarević, Milica M. and Ugrinović, Vukašin Đ. and Pjanović, Rada V. and Obradović, Bojana M. and Kalagasidis Krušić, Melina T.",
year = "2023",
abstract = "Everyday struggle of humanity with novel diseases and present once, urge researchers to find novel and improve existing therapies to enhance their efficiency and safety. One of the promising approaches to overcome these challenges is controlled release of drugs. Biomaterials based on poly(methacrylic acid) (PMAA) are excellent drug delivery systems because they can control release rate and released amount of drug. Also, due to their pH sensitivity the PMAA hydrogels can release drug at the site of action. Namely, these nontoxic and biocompatible hydrogels swell in the environment with pH value higher than pKa of PMAA (4.6) and release encapsulated drug during the process. In present study, PMAA hydrogels are synthetized under ambient conditions by simple, cost effective and eco-friendly synthesis. Novel initiation system based on hydrogen peroxide, potato peel peroxidase and vitamin C (VC) is used for the first time for free radical polymerization of PMAA hydrogel. In accordance with the principles of circular economy, peroxidase was isolated from potato peel waste by water extraction for 12 h at 4 ℃. Four PMAA hydrogels were prepared by using potato peel peroxidase with various enzyme activity (0.4; 0.8; 1.2 and 1.8 IU), whereas the amounts of H2O2 (30 mL) and VC (10 mg) have been kept constant. The composition of the PMAA hydrogels was confirmed by FTIR analysis, whereas their porous structure was revealed by SEM. The swelling of the PMAA hydrogels was monitored in two media: 0.1M HCl (as simulation of human stomach) and phosphate buffer with pH 7.4 (as simulation of human intestines). In order to encapsulate anti-inflammatory drug – dexamethasone into the PMAA hydrogels, the hydrogels were immersed into the dexamethasone aqueous solution (5 mg/ml) and left to swell to the equilibrium, after which they were dried at room temperature. Dexamethasone release from the PMAA hydrogels was monitored in the same environments as was the PMAA swelling. The results showed that around seven times higher amount of dexamethasone was released in the medium with pH 7.4 than in the medium with pH 1. Present study has showed that the PMAA hydrogels, prepared through green and eco-friendly method, have huge potential for encapsulation and controlled release of anti-inflammatory drugs and therefore, for the treatment of rheumatoid arthritis.",
publisher = "AO Foundation",
journal = "ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical",
title = "Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs",
pages = "572",
volume = "3",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6620"
}

Marković, M. D., Svetozarević, M. M., Ugrinović, V. Đ., Pjanović, R. V., Obradović, B. M.,& Kalagasidis Krušić, M. T.. (2023). Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs. in ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical
AO Foundation., 3, 572.
https://hdl.handle.net/21.15107/rcub_technorep_6620

Marković MD, Svetozarević MM, Ugrinović VĐ, Pjanović RV, Obradović BM, Kalagasidis Krušić MT. Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs. in ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical. 2023;3:572.
https://hdl.handle.net/21.15107/rcub_technorep_6620 .

Marković, Maja D., Svetozarević, Milica M., Ugrinović, Vukašin Đ., Pjanović, Rada V., Obradović, Bojana M., Kalagasidis Krušić, Melina T., "Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs" in ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical, 3 (2023):572,
https://hdl.handle.net/21.15107/rcub_technorep_6620 .

TY  - CONF
AU  - Marković, Maja D.
AU  - Miladinović, Nina N.
AU  - Savić, Sanja I.
AU  - Panić, Vesna V.
AU  - Pantić, Olga J.
AU  - Spasojević, Pavle M.
AU  - Pjanović, Rada V.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7300
AB  - Controlled release of drugs is a promising tool for improvement of therapy for many 
diseases because drug concentration for long period of time is kept constant, whereas
number of drug doses and side effects are reduced. pH sensitive hydrogels, such as 
hydrogels based on poly(methacrylic acid) (PMAA), are extensively employed for 
drug delivery. The synthesis of the hydrogel is one of the most important factors
which affect encapsulation of drug and its bioactivity. In present study, we investigate 
if the encapsulation of poorly water-soluble active substance (caffeine) during the 
synthesis of PMAA hydrogels, and later its controlled release from the PMAA
hydrogels, can be achieved by applying novel ”green” method for the hydrogels 
preparation. The PMAA hydrogels with encapsulated caffeine and various amount of 
crosslinker are prepared by free radical polymerisation in aqueous solution and 
ambient conditions by using novel initiation system based on hydrogen peroxide and 
vitamin C. The swelling behaviour of the PMAA hydrogels and controlled release of 
caffeine are investigated in two media with pH 1 and pH 6.8 as simulation of human 
stomach and intestines, respectively. The SDeq values of the PMAA hydrogels are
eight times higher in the medium with pH 6.8 and as a consequence, three times higher
amount of caffeine is released in that medium. The increase in the crosslinker amount 
leads to the decrease of SDeq values of the PMAA hydrogels and amount of released 
caffeine. The results show that the PMAA hydrogels have great potential for controlled
release of poorly water-soluble drugs.
PB  - Leskovac : Faculty of Technology
C3  - Book of abstracts / 15th International Symposium "Novel Technologies and Sustainable Development" Leskovac, October, 20-21, 2023
T1  - POLY(METHACRYLIC ACID) HYDROGELS PREPARED BY ”GREEN”  METHOD: SWELLING PROPERTIES AND CONTROLLED RELEASE OF  CAFFEINE
SP  - 97
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7300
ER  - 

@conference{
author = "Marković, Maja D. and Miladinović, Nina N. and Savić, Sanja I. and Panić, Vesna V. and Pantić, Olga J. and Spasojević, Pavle M. and Pjanović, Rada V.",
year = "2023",
abstract = "Controlled release of drugs is a promising tool for improvement of therapy for many 
diseases because drug concentration for long period of time is kept constant, whereas
number of drug doses and side effects are reduced. pH sensitive hydrogels, such as 
hydrogels based on poly(methacrylic acid) (PMAA), are extensively employed for 
drug delivery. The synthesis of the hydrogel is one of the most important factors
which affect encapsulation of drug and its bioactivity. In present study, we investigate 
if the encapsulation of poorly water-soluble active substance (caffeine) during the 
synthesis of PMAA hydrogels, and later its controlled release from the PMAA
hydrogels, can be achieved by applying novel ”green” method for the hydrogels 
preparation. The PMAA hydrogels with encapsulated caffeine and various amount of 
crosslinker are prepared by free radical polymerisation in aqueous solution and 
ambient conditions by using novel initiation system based on hydrogen peroxide and 
vitamin C. The swelling behaviour of the PMAA hydrogels and controlled release of 
caffeine are investigated in two media with pH 1 and pH 6.8 as simulation of human 
stomach and intestines, respectively. The SDeq values of the PMAA hydrogels are
eight times higher in the medium with pH 6.8 and as a consequence, three times higher
amount of caffeine is released in that medium. The increase in the crosslinker amount 
leads to the decrease of SDeq values of the PMAA hydrogels and amount of released 
caffeine. The results show that the PMAA hydrogels have great potential for controlled
release of poorly water-soluble drugs.",
publisher = "Leskovac : Faculty of Technology",
journal = "Book of abstracts / 15th International Symposium "Novel Technologies and Sustainable Development" Leskovac, October, 20-21, 2023",
title = "POLY(METHACRYLIC ACID) HYDROGELS PREPARED BY ”GREEN”  METHOD: SWELLING PROPERTIES AND CONTROLLED RELEASE OF  CAFFEINE",
pages = "97",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7300"
}

Marković, M. D., Miladinović, N. N., Savić, S. I., Panić, V. V., Pantić, O. J., Spasojević, P. M.,& Pjanović, R. V.. (2023). POLY(METHACRYLIC ACID) HYDROGELS PREPARED BY ”GREEN”  METHOD: SWELLING PROPERTIES AND CONTROLLED RELEASE OF  CAFFEINE. in Book of abstracts / 15th International Symposium "Novel Technologies and Sustainable Development" Leskovac, October, 20-21, 2023
Leskovac : Faculty of Technology., 97.
https://hdl.handle.net/21.15107/rcub_technorep_7300

Marković MD, Miladinović NN, Savić SI, Panić VV, Pantić OJ, Spasojević PM, Pjanović RV. POLY(METHACRYLIC ACID) HYDROGELS PREPARED BY ”GREEN”  METHOD: SWELLING PROPERTIES AND CONTROLLED RELEASE OF  CAFFEINE. in Book of abstracts / 15th International Symposium "Novel Technologies and Sustainable Development" Leskovac, October, 20-21, 2023. 2023;:97.
https://hdl.handle.net/21.15107/rcub_technorep_7300 .

Marković, Maja D., Miladinović, Nina N., Savić, Sanja I., Panić, Vesna V., Pantić, Olga J., Spasojević, Pavle M., Pjanović, Rada V., "POLY(METHACRYLIC ACID) HYDROGELS PREPARED BY ”GREEN”  METHOD: SWELLING PROPERTIES AND CONTROLLED RELEASE OF  CAFFEINE" in Book of abstracts / 15th International Symposium "Novel Technologies and Sustainable Development" Leskovac, October, 20-21, 2023 (2023):97,
https://hdl.handle.net/21.15107/rcub_technorep_7300 .

TY  - CONF
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Blagojević, Stevan
AU  - Radulović, Aleksandra
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6947
AB  - Blackcurrant is anthocyanin-rich berry with proven antioxidant and photoprotective
activity and emerging prebiotic potential, widely applied in cosmetic products. Although
beneficial effects of its extract are well known, release from skin care products and
(trans)dermal permeation were not previously investigated.
In this study, enzyme-assisted extraction of polyphenols from lyophilized
blackcurrant was performed and obtained extract was incorporated into an Aristoflex® AVC
based hydrogel. Furthermore, Franz diffusion cell experiments were conducted in order to
examine the release of these molecules from the prepared formulation using cellulose acetate
membrane and transdermal and dermal delivery using human skin mimicking membrane
(Strat® M).
Obtained results revealed that all four dominant anthocyanins readily permeated from
hydrogel since 17.5%, 32.8%, 33.8% and 39.2% of delphinidin 3-glucoside, delphinidin 3-
rutinoside, cyanidin 3-glucoside and cyanidin 3-rutinoside, respectively, were detected in
receptor fluid after 24 h. Experimental values were successfully fitted with the Peppas and
Sahlin diffusion model. On the other hand, after 72 h long experiment with transdermal skin
diffusion model (Strat® M membrane), no detectable amounts of anthocyanins were present
in receptor fluid and only 0.5% of the initial quantity from donor compartment was extracted
from the membrane itself, indicating weak dermal delivery.
Present study revealed that hydrogel is suitable carrier system for the topical delivery
of bioactive anthocyanins from blackcurrant, while dermal and transdermal delivery of these
molecules is very limited. This implies applicability of blackcurrant extract for treatments
targeting skin surface (i.e. antioxidant, prebiotic, photoprotective).
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - IN VITRO RELEASE AND (TRANS)DERMAL DELIVERY STUDY OF BLACKCURRANT ANTHOCYANINS INCORPORATED IN COSMETIC FORMULATION
SP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6947
ER  - 

@conference{
author = "Petrov Ivanković, Anja and Ćorović, Marija and Milivojević, Ana and Blagojević, Stevan and Radulović, Aleksandra and Pjanović, Rada and Bezbradica, Dejan",
year = "2023",
abstract = "Blackcurrant is anthocyanin-rich berry with proven antioxidant and photoprotective
activity and emerging prebiotic potential, widely applied in cosmetic products. Although
beneficial effects of its extract are well known, release from skin care products and
(trans)dermal permeation were not previously investigated.
In this study, enzyme-assisted extraction of polyphenols from lyophilized
blackcurrant was performed and obtained extract was incorporated into an Aristoflex® AVC
based hydrogel. Furthermore, Franz diffusion cell experiments were conducted in order to
examine the release of these molecules from the prepared formulation using cellulose acetate
membrane and transdermal and dermal delivery using human skin mimicking membrane
(Strat® M).
Obtained results revealed that all four dominant anthocyanins readily permeated from
hydrogel since 17.5%, 32.8%, 33.8% and 39.2% of delphinidin 3-glucoside, delphinidin 3-
rutinoside, cyanidin 3-glucoside and cyanidin 3-rutinoside, respectively, were detected in
receptor fluid after 24 h. Experimental values were successfully fitted with the Peppas and
Sahlin diffusion model. On the other hand, after 72 h long experiment with transdermal skin
diffusion model (Strat® M membrane), no detectable amounts of anthocyanins were present
in receptor fluid and only 0.5% of the initial quantity from donor compartment was extracted
from the membrane itself, indicating weak dermal delivery.
Present study revealed that hydrogel is suitable carrier system for the topical delivery
of bioactive anthocyanins from blackcurrant, while dermal and transdermal delivery of these
molecules is very limited. This implies applicability of blackcurrant extract for treatments
targeting skin surface (i.e. antioxidant, prebiotic, photoprotective).",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "IN VITRO RELEASE AND (TRANS)DERMAL DELIVERY STUDY OF BLACKCURRANT ANTHOCYANINS INCORPORATED IN COSMETIC FORMULATION",
pages = "30",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6947"
}

Petrov Ivanković, A., Ćorović, M., Milivojević, A., Blagojević, S., Radulović, A., Pjanović, R.,& Bezbradica, D.. (2023). IN VITRO RELEASE AND (TRANS)DERMAL DELIVERY STUDY OF BLACKCURRANT ANTHOCYANINS INCORPORATED IN COSMETIC FORMULATION. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 30.
https://hdl.handle.net/21.15107/rcub_technorep_6947

Petrov Ivanković A, Ćorović M, Milivojević A, Blagojević S, Radulović A, Pjanović R, Bezbradica D. IN VITRO RELEASE AND (TRANS)DERMAL DELIVERY STUDY OF BLACKCURRANT ANTHOCYANINS INCORPORATED IN COSMETIC FORMULATION. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:30.
https://hdl.handle.net/21.15107/rcub_technorep_6947 .

Petrov Ivanković, Anja, Ćorović, Marija, Milivojević, Ana, Blagojević, Stevan, Radulović, Aleksandra, Pjanović, Rada, Bezbradica, Dejan, "IN VITRO RELEASE AND (TRANS)DERMAL DELIVERY STUDY OF BLACKCURRANT ANTHOCYANINS INCORPORATED IN COSMETIC FORMULATION" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):30,
https://hdl.handle.net/21.15107/rcub_technorep_6947 .

TY  - JOUR
AU  - Marković, Maja D.
AU  - Panić, Vesna V.
AU  - Savić, Sanja I.
AU  - Ugrinović, Vukašin Đ.
AU  - Pjanović, Rada V.
AU  - Spasojević, Milica M.
AU  - Spasojević, Pavle M.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5092
AB  - Materials sensitive to external stimuli are recognized as safe and effective tool able to respond to specific demands in the therapy of various diseases. Thermo sensitive hydrogels based on poly(N-isopropylacrylamide) (P(NIPAAM)) are widely investigated for targeted drug delivery. Still, the abundance of the stimuli in the human body often requires more than one responsive group able to act simultaneously to achieve optimal therapeutic effects. Due to its pH sensitivity and bio-based production, crotonic acid (CA) was a monomer of choice for preparation of eco-friendly copolymer hydrogels based on NIPAAM and CA (P(NIPAAMcoCA)), which turned to be thermo and pH sensitive at the same time. The potential of the P(NIPAAMcoCA) system for encapsulation and controlled release of drugs with different solubility was investigated engaging water-soluble lidocaine hydrochloride and poorly water-soluble ibuprofen as model drugs. The hydrogels were characterized by various technics: FTIR, DSC, SEM and single compressive tests, while swelling behavior and controlled release of the drugs were analyzed with respect to the CA amount in two environments with different pH values at 25 °C and 37 °C. It was demonstrated that due to their dual responsiveness the P(NIPAAMcoCA) hydrogels have potential for controlled release of drugs with different solubility.
PB  - Elsevier
T2  - Microporous and Mesoporous Materials
T1  - Biobased thermo/pH sensitive poly(N-isopropylacrylamide-co-crotonic acid) hydrogels for targeted drug delivery
SP  - 111817
VL  - 335
DO  - 10.1016/j.micromeso.2022.111817
ER  - 

@article{
author = "Marković, Maja D. and Panić, Vesna V. and Savić, Sanja I. and Ugrinović, Vukašin Đ. and Pjanović, Rada V. and Spasojević, Milica M. and Spasojević, Pavle M.",
year = "2022",
abstract = "Materials sensitive to external stimuli are recognized as safe and effective tool able to respond to specific demands in the therapy of various diseases. Thermo sensitive hydrogels based on poly(N-isopropylacrylamide) (P(NIPAAM)) are widely investigated for targeted drug delivery. Still, the abundance of the stimuli in the human body often requires more than one responsive group able to act simultaneously to achieve optimal therapeutic effects. Due to its pH sensitivity and bio-based production, crotonic acid (CA) was a monomer of choice for preparation of eco-friendly copolymer hydrogels based on NIPAAM and CA (P(NIPAAMcoCA)), which turned to be thermo and pH sensitive at the same time. The potential of the P(NIPAAMcoCA) system for encapsulation and controlled release of drugs with different solubility was investigated engaging water-soluble lidocaine hydrochloride and poorly water-soluble ibuprofen as model drugs. The hydrogels were characterized by various technics: FTIR, DSC, SEM and single compressive tests, while swelling behavior and controlled release of the drugs were analyzed with respect to the CA amount in two environments with different pH values at 25 °C and 37 °C. It was demonstrated that due to their dual responsiveness the P(NIPAAMcoCA) hydrogels have potential for controlled release of drugs with different solubility.",
publisher = "Elsevier",
journal = "Microporous and Mesoporous Materials",
title = "Biobased thermo/pH sensitive poly(N-isopropylacrylamide-co-crotonic acid) hydrogels for targeted drug delivery",
pages = "111817",
volume = "335",
doi = "10.1016/j.micromeso.2022.111817"
}

Marković, M. D., Panić, V. V., Savić, S. I., Ugrinović, V. Đ., Pjanović, R. V., Spasojević, M. M.,& Spasojević, P. M.. (2022). Biobased thermo/pH sensitive poly(N-isopropylacrylamide-co-crotonic acid) hydrogels for targeted drug delivery. in Microporous and Mesoporous Materials
Elsevier., 335, 111817.
https://doi.org/10.1016/j.micromeso.2022.111817

Marković MD, Panić VV, Savić SI, Ugrinović VĐ, Pjanović RV, Spasojević MM, Spasojević PM. Biobased thermo/pH sensitive poly(N-isopropylacrylamide-co-crotonic acid) hydrogels for targeted drug delivery. in Microporous and Mesoporous Materials. 2022;335:111817.
doi:10.1016/j.micromeso.2022.111817 .

Marković, Maja D., Panić, Vesna V., Savić, Sanja I., Ugrinović, Vukašin Đ., Pjanović, Rada V., Spasojević, Milica M., Spasojević, Pavle M., "Biobased thermo/pH sensitive poly(N-isopropylacrylamide-co-crotonic acid) hydrogels for targeted drug delivery" in Microporous and Mesoporous Materials, 335 (2022):111817,
https://doi.org/10.1016/j.micromeso.2022.111817 . .

TY  - CONF
AU  - Miladinović, Nina N.
AU  - Marković, Maja
AU  - Pjanović, Rada
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5228
AB  - Several million tons of orange peel are generated annually as a result of the industrial
processing of this fruit. Orange peels are rich in polyphenols, that show antioxidant
activity. Hence, converting orange peels into antioxidant extract by using green eutectic
solvents (GES) would be a good solution. GES represent a mixture of organic substances
that are not harmful to human health, environmentally friendly and highly selective
towards the targeted active substance. The aim of this work was the extraction of
polyphenols from orange peel waste using a GES based on urea and glycerol, their
encapsulation into hydrogels based on poly(methacrylic acid) (PMAA), and controlled
release from these hydrogels. PMAA are non-toxic, biocompatible and pH sensitive, so
they are widely used for the targeted delivery of active substances. Swelling of PMAA
and the controlled release of polyphenols from PMAA were monitored in two mediums
with different pH values: 0.1 M hydrochloric acid (pH=1) and phosphate buffer
(pH=6.8) at 37 °C. The influence of the crosslinker amount and neutralization degree of
methacrylic acid (ND) on the PMAA swelling and the release of polyphenols was
examined. The kinetics of PMAA swelling and polyphenol release were analyzed using
the Peppas model. It was concluded that the swelling degree of PMAA increased with
an increase in pH value of medium and ND, and decreased with an increase in crosslinker
amount. As a consequence, the cumulative release of polyphenols was around three
times higher in pH=6.8 than in pH=1 for both series of the samples. Peppas model
showed that diffusion was the primary mechanism of polyphenols release for most
samples. Based on the analysis of the results, it is concluded that the sample with optimal
characteristics for the controlled release of polyphenols is the sample with 0%
neutralized methacrylic acid and 0.4 mol% MBA.
PB  - Serbian Chemical Society and Serbian Young Chemists’ Club, Belgrade, Serbia
C3  - 8th Conference of Young Chemists of Serbia Book of Abstract
T1  - Extraction of polyphenols from orange peel and their controlled release from pH-sensitive hydrogels
SP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5228
ER  - 

@conference{
author = "Miladinović, Nina N. and Marković, Maja and Pjanović, Rada",
year = "2022",
abstract = "Several million tons of orange peel are generated annually as a result of the industrial
processing of this fruit. Orange peels are rich in polyphenols, that show antioxidant
activity. Hence, converting orange peels into antioxidant extract by using green eutectic
solvents (GES) would be a good solution. GES represent a mixture of organic substances
that are not harmful to human health, environmentally friendly and highly selective
towards the targeted active substance. The aim of this work was the extraction of
polyphenols from orange peel waste using a GES based on urea and glycerol, their
encapsulation into hydrogels based on poly(methacrylic acid) (PMAA), and controlled
release from these hydrogels. PMAA are non-toxic, biocompatible and pH sensitive, so
they are widely used for the targeted delivery of active substances. Swelling of PMAA
and the controlled release of polyphenols from PMAA were monitored in two mediums
with different pH values: 0.1 M hydrochloric acid (pH=1) and phosphate buffer
(pH=6.8) at 37 °C. The influence of the crosslinker amount and neutralization degree of
methacrylic acid (ND) on the PMAA swelling and the release of polyphenols was
examined. The kinetics of PMAA swelling and polyphenol release were analyzed using
the Peppas model. It was concluded that the swelling degree of PMAA increased with
an increase in pH value of medium and ND, and decreased with an increase in crosslinker
amount. As a consequence, the cumulative release of polyphenols was around three
times higher in pH=6.8 than in pH=1 for both series of the samples. Peppas model
showed that diffusion was the primary mechanism of polyphenols release for most
samples. Based on the analysis of the results, it is concluded that the sample with optimal
characteristics for the controlled release of polyphenols is the sample with 0%
neutralized methacrylic acid and 0.4 mol% MBA.",
publisher = "Serbian Chemical Society and Serbian Young Chemists’ Club, Belgrade, Serbia",
journal = "8th Conference of Young Chemists of Serbia Book of Abstract",
title = "Extraction of polyphenols from orange peel and their controlled release from pH-sensitive hydrogels",
pages = "88",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5228"
}

Miladinović, N. N., Marković, M.,& Pjanović, R.. (2022). Extraction of polyphenols from orange peel and their controlled release from pH-sensitive hydrogels. in 8th Conference of Young Chemists of Serbia Book of Abstract
Serbian Chemical Society and Serbian Young Chemists’ Club, Belgrade, Serbia., 88.
https://hdl.handle.net/21.15107/rcub_technorep_5228

Miladinović NN, Marković M, Pjanović R. Extraction of polyphenols from orange peel and their controlled release from pH-sensitive hydrogels. in 8th Conference of Young Chemists of Serbia Book of Abstract. 2022;:88.
https://hdl.handle.net/21.15107/rcub_technorep_5228 .

Miladinović, Nina N., Marković, Maja, Pjanović, Rada, "Extraction of polyphenols from orange peel and their controlled release from pH-sensitive hydrogels" in 8th Conference of Young Chemists of Serbia Book of Abstract (2022):88,
https://hdl.handle.net/21.15107/rcub_technorep_5228 .

TY  - CONF
AU  - Marković, Maja D.
AU  - Panić, Vesna V.
AU  - Pjanović, Rada V.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5477
AB  - Researchers are making everyday efforts to develop new drugs or improve present ones in order
to enhance therapies of various diseases, especially serious ones like cancer. Drug delivery
systems (DDS) are one of the solutions for safer and more efficient therapy. Hydrogels based
on poly(methacrylic acid) (PMAA) are extensively investigated as DDS due to their nontoxicity, biocompatibility and pH sensitivity. Many chemotherapeutics are poorly watersoluble, so it is quite challenging to encapsulate them into highly hydrophilic PMAA. In our
previous study we overcome this limitation by modifying PMAA with amphiphilic casein and
demonstrated that poorly water-soluble model drug – caffeine can be successfully encapsulated
and released in control manner from these samples (H hydrogels). In present study we go step
forward and investigated how the change in the amount of encapsulated caffeine affect the
mechanism of caffeine release from the H hydrogels in medium with pH of 6.8 (which simulates
the environment in human intestines). Commonly used models for the analysis of kinetics of
drug release from hydrogels: Ritger-Peppas, Higuchi and Kopcha model are employed for the
analysis of the mechanism of caffeine release. Presented results indicate that it is possible to
adjust the manner and mechanism of drug release by changing the amount of encapsulated drug,
due to which the H hydrogels can adapt to the unique requirements of the therapy.
PB  - University of Szeged
C3  - PROCEEDINGS OF THE 28th International Symposium on Analytical and Environmental Problems
T1  - THE EFFECT OF ENCAPSULATED AMOUNT OF CAFFEINE ON THE MECHANISM OF ITS RELEASE FROM HYDROGELS BASED ON POLY(METHACRYLIC ACID) AND CASEIN
EP  - 226
SP  - 221
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5477
ER  - 

@conference{
author = "Marković, Maja D. and Panić, Vesna V. and Pjanović, Rada V.",
year = "2022",
abstract = "Researchers are making everyday efforts to develop new drugs or improve present ones in order
to enhance therapies of various diseases, especially serious ones like cancer. Drug delivery
systems (DDS) are one of the solutions for safer and more efficient therapy. Hydrogels based
on poly(methacrylic acid) (PMAA) are extensively investigated as DDS due to their nontoxicity, biocompatibility and pH sensitivity. Many chemotherapeutics are poorly watersoluble, so it is quite challenging to encapsulate them into highly hydrophilic PMAA. In our
previous study we overcome this limitation by modifying PMAA with amphiphilic casein and
demonstrated that poorly water-soluble model drug – caffeine can be successfully encapsulated
and released in control manner from these samples (H hydrogels). In present study we go step
forward and investigated how the change in the amount of encapsulated caffeine affect the
mechanism of caffeine release from the H hydrogels in medium with pH of 6.8 (which simulates
the environment in human intestines). Commonly used models for the analysis of kinetics of
drug release from hydrogels: Ritger-Peppas, Higuchi and Kopcha model are employed for the
analysis of the mechanism of caffeine release. Presented results indicate that it is possible to
adjust the manner and mechanism of drug release by changing the amount of encapsulated drug,
due to which the H hydrogels can adapt to the unique requirements of the therapy.",
publisher = "University of Szeged",
journal = "PROCEEDINGS OF THE 28th International Symposium on Analytical and Environmental Problems",
title = "THE EFFECT OF ENCAPSULATED AMOUNT OF CAFFEINE ON THE MECHANISM OF ITS RELEASE FROM HYDROGELS BASED ON POLY(METHACRYLIC ACID) AND CASEIN",
pages = "226-221",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5477"
}

Marković, M. D., Panić, V. V.,& Pjanović, R. V.. (2022). THE EFFECT OF ENCAPSULATED AMOUNT OF CAFFEINE ON THE MECHANISM OF ITS RELEASE FROM HYDROGELS BASED ON POLY(METHACRYLIC ACID) AND CASEIN. in PROCEEDINGS OF THE 28th International Symposium on Analytical and Environmental Problems
University of Szeged., 221-226.
https://hdl.handle.net/21.15107/rcub_technorep_5477

Marković MD, Panić VV, Pjanović RV. THE EFFECT OF ENCAPSULATED AMOUNT OF CAFFEINE ON THE MECHANISM OF ITS RELEASE FROM HYDROGELS BASED ON POLY(METHACRYLIC ACID) AND CASEIN. in PROCEEDINGS OF THE 28th International Symposium on Analytical and Environmental Problems. 2022;:221-226.
https://hdl.handle.net/21.15107/rcub_technorep_5477 .

Marković, Maja D., Panić, Vesna V., Pjanović, Rada V., "THE EFFECT OF ENCAPSULATED AMOUNT OF CAFFEINE ON THE MECHANISM OF ITS RELEASE FROM HYDROGELS BASED ON POLY(METHACRYLIC ACID) AND CASEIN" in PROCEEDINGS OF THE 28th International Symposium on Analytical and Environmental Problems (2022):221-226,
https://hdl.handle.net/21.15107/rcub_technorep_5477 .

TY  - JOUR
AU  - Pantic, Olga J.
AU  - Pjanovic, Rada V.
AU  - Panić, Vesna V.
AU  - Savic, Sanja I.
AU  - Spasojevic, Pavle M.
AU  - Marković, Maja D.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5479
AB  - Drug delivery system is an amazing tool which is widely used for drug
protection and its controlled release in order to enhance drug bioavailability,
reduce side effects and therefore to improve overall therapy. Hydrogels have
been attracted great attention as drug carriers due to their great physicochemical
properties, similarity to the living tissues and biocompatibility. One group of
pH sensitive hydrogels are based on poly(methacrylic acid) (PMAA). These
non-toxic hydrogels are used as drug delivery system because they swell as
a response to the change in pH of external environment and drug is being
released during the process. In present study, in order to improve the control of
drug release rate, caffeine was encapsulated in liposomes which were further
embedded into PMC hydrogel (PMCL). It was investigated how the change in
neutralization degree of methacrylic acid affect the swelling degree of PMCL
hydrogels and caffeine release in two environments at 37 °C for 24 h: 0.1 M
hydrochloride acid (pH 1) and phosphate buffer with pH value of 6.8 (pH 6.8), as
a simulation of pH environment in human stomach and intestines, respectively.
Obtained results show that PMCL hydrogels have great potential for controlled
release of poorly water-soluble drugs in human intestines.
PB  - the Faculty of Technology, University of Banja Luka
T2  - Journal of Chemists, Technologists and Environmentalists
T1  - Effect of neutralization degree of methacrylic acid on hydrogel swelling and drug release
EP  - 5
IS  - 1
SP  - 1
VL  - 3
DO  - 10.7251/JCTE2203001P
ER  - 

@article{
author = "Pantic, Olga J. and Pjanovic, Rada V. and Panić, Vesna V. and Savic, Sanja I. and Spasojevic, Pavle M. and Marković, Maja D.",
year = "2022",
abstract = "Drug delivery system is an amazing tool which is widely used for drug
protection and its controlled release in order to enhance drug bioavailability,
reduce side effects and therefore to improve overall therapy. Hydrogels have
been attracted great attention as drug carriers due to their great physicochemical
properties, similarity to the living tissues and biocompatibility. One group of
pH sensitive hydrogels are based on poly(methacrylic acid) (PMAA). These
non-toxic hydrogels are used as drug delivery system because they swell as
a response to the change in pH of external environment and drug is being
released during the process. In present study, in order to improve the control of
drug release rate, caffeine was encapsulated in liposomes which were further
embedded into PMC hydrogel (PMCL). It was investigated how the change in
neutralization degree of methacrylic acid affect the swelling degree of PMCL
hydrogels and caffeine release in two environments at 37 °C for 24 h: 0.1 M
hydrochloride acid (pH 1) and phosphate buffer with pH value of 6.8 (pH 6.8), as
a simulation of pH environment in human stomach and intestines, respectively.
Obtained results show that PMCL hydrogels have great potential for controlled
release of poorly water-soluble drugs in human intestines.",
publisher = "the Faculty of Technology, University of Banja Luka",
journal = "Journal of Chemists, Technologists and Environmentalists",
title = "Effect of neutralization degree of methacrylic acid on hydrogel swelling and drug release",
pages = "5-1",
number = "1",
volume = "3",
doi = "10.7251/JCTE2203001P"
}

Pantic, O. J., Pjanovic, R. V., Panić, V. V., Savic, S. I., Spasojevic, P. M.,& Marković, M. D.. (2022). Effect of neutralization degree of methacrylic acid on hydrogel swelling and drug release. in Journal of Chemists, Technologists and Environmentalists
the Faculty of Technology, University of Banja Luka., 3(1), 1-5.
https://doi.org/10.7251/JCTE2203001P

Pantic OJ, Pjanovic RV, Panić VV, Savic SI, Spasojevic PM, Marković MD. Effect of neutralization degree of methacrylic acid on hydrogel swelling and drug release. in Journal of Chemists, Technologists and Environmentalists. 2022;3(1):1-5.
doi:10.7251/JCTE2203001P .

Pantic, Olga J., Pjanovic, Rada V., Panić, Vesna V., Savic, Sanja I., Spasojevic, Pavle M., Marković, Maja D., "Effect of neutralization degree of methacrylic acid on hydrogel swelling and drug release" in Journal of Chemists, Technologists and Environmentalists, 3, no. 1 (2022):1-5,
https://doi.org/10.7251/JCTE2203001P . .

TY  - CONF
AU  - Marković, Maja D.
AU  - Pjanović, Rada V.
AU  - Spasojević, Pavle M.
AU  - Savić, Sanja I.
AU  - Panić, Vesna V.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5643
AB  - Everyday demands for safer and more efficient therapy for many diseases, especially serious ones such as various types of cancer, put various challenges in front of modern science. One of them lies in the fact that numerous anticancer drugs are poorly-water soluble and therefore their encap-sulation and controlled release are quite demanding processes. In the present study, we deepened our research of carrier based on hydrophilic poly(methacrylic acid) modified with amphiphilic ca-sein, in which poorly water-soluble model drug caffeine was encapsulated (PMAC-Caf hydrogels). It was investigated how neutralization degree of methacrylic acid (MAA) and amount of encapsulated caffeine affected swelling behavior of the PMAC-Caf hydrogels and caffeine release. Easy, one pot, simultaneous synthesis of the carrier and the encapsulation of caffeine is presented, obtaining thereby as prepared PMAC-Caf drug delivery system that could respond to the specific demands of the tar-geted delivery of the poorly water-soluble drug - protecting it in the environment which simulated human stomach and releasing it in the environment which simulated human intestines. Changing the synthesis parameters (neutralization degree of MAA and/or amount of encapsulated caffeine) we achieved controlled release of caffeine, indicating that the number of the required doses of the drug in the treatment and its side effects could be reduced. Results showed that the PMAC-Caf drug deliv-ery systems have huge potential for controlled release of poorly water-soluble drugs.
PB  - Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku
C3  - Zbornik radova 35. Međunarodnog kongresa o procesnoj industriji
T1  - CONTROLLED RELEASE OF CAFFEINE FROM THREE DIMENSIONAL NETWORKS BASED ON POLY(METACRYLIC ACID) AND CASEIN - ANALYSIS OF THE EFFECT OF CAFFEINE CONCENTRATION ON RELEASE PROCESS
EP  - 24
IS  - 1
SP  - 19
VL  - 35
DO  - https://doi.org/10.24094/ptk.022.019
ER  - 

@conference{
author = "Marković, Maja D. and Pjanović, Rada V. and Spasojević, Pavle M. and Savić, Sanja I. and Panić, Vesna V.",
year = "2022",
abstract = "Everyday demands for safer and more efficient therapy for many diseases, especially serious ones such as various types of cancer, put various challenges in front of modern science. One of them lies in the fact that numerous anticancer drugs are poorly-water soluble and therefore their encap-sulation and controlled release are quite demanding processes. In the present study, we deepened our research of carrier based on hydrophilic poly(methacrylic acid) modified with amphiphilic ca-sein, in which poorly water-soluble model drug caffeine was encapsulated (PMAC-Caf hydrogels). It was investigated how neutralization degree of methacrylic acid (MAA) and amount of encapsulated caffeine affected swelling behavior of the PMAC-Caf hydrogels and caffeine release. Easy, one pot, simultaneous synthesis of the carrier and the encapsulation of caffeine is presented, obtaining thereby as prepared PMAC-Caf drug delivery system that could respond to the specific demands of the tar-geted delivery of the poorly water-soluble drug - protecting it in the environment which simulated human stomach and releasing it in the environment which simulated human intestines. Changing the synthesis parameters (neutralization degree of MAA and/or amount of encapsulated caffeine) we achieved controlled release of caffeine, indicating that the number of the required doses of the drug in the treatment and its side effects could be reduced. Results showed that the PMAC-Caf drug deliv-ery systems have huge potential for controlled release of poorly water-soluble drugs.",
publisher = "Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku",
journal = "Zbornik radova 35. Međunarodnog kongresa o procesnoj industriji",
title = "CONTROLLED RELEASE OF CAFFEINE FROM THREE DIMENSIONAL NETWORKS BASED ON POLY(METACRYLIC ACID) AND CASEIN - ANALYSIS OF THE EFFECT OF CAFFEINE CONCENTRATION ON RELEASE PROCESS",
pages = "24-19",
number = "1",
volume = "35",
doi = "https://doi.org/10.24094/ptk.022.019"
}

Marković, M. D., Pjanović, R. V., Spasojević, P. M., Savić, S. I.,& Panić, V. V.. (2022). CONTROLLED RELEASE OF CAFFEINE FROM THREE DIMENSIONAL NETWORKS BASED ON POLY(METACRYLIC ACID) AND CASEIN - ANALYSIS OF THE EFFECT OF CAFFEINE CONCENTRATION ON RELEASE PROCESS. in Zbornik radova 35. Međunarodnog kongresa o procesnoj industriji
Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku., 35(1), 19-24.
https://doi.org/https://doi.org/10.24094/ptk.022.019

Marković MD, Pjanović RV, Spasojević PM, Savić SI, Panić VV. CONTROLLED RELEASE OF CAFFEINE FROM THREE DIMENSIONAL NETWORKS BASED ON POLY(METACRYLIC ACID) AND CASEIN - ANALYSIS OF THE EFFECT OF CAFFEINE CONCENTRATION ON RELEASE PROCESS. in Zbornik radova 35. Međunarodnog kongresa o procesnoj industriji. 2022;35(1):19-24.
doi:https://doi.org/10.24094/ptk.022.019 .

Marković, Maja D., Pjanović, Rada V., Spasojević, Pavle M., Savić, Sanja I., Panić, Vesna V., "CONTROLLED RELEASE OF CAFFEINE FROM THREE DIMENSIONAL NETWORKS BASED ON POLY(METACRYLIC ACID) AND CASEIN - ANALYSIS OF THE EFFECT OF CAFFEINE CONCENTRATION ON RELEASE PROCESS" in Zbornik radova 35. Međunarodnog kongresa o procesnoj industriji, 35, no. 1 (2022):19-24,
https://doi.org/https://doi.org/10.24094/ptk.022.019 . .

TY  - JOUR
AU  - Šeremet, Danijela
AU  - Vugrinec, Kristina
AU  - Petrović, Predrag
AU  - Butorac, Ana
AU  - Kuzmić, Sunčica
AU  - Vojvodić Cebin, Aleksandra
AU  - Mandura, Ana
AU  - Lovrić, Marija
AU  - Pjanović, Rada
AU  - Komes, Draženka
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4987
AB  - Conventional and innovative (microwave-assisted and subcritical water extraction) techniques were applied to investigate the bioactive content of traditional plant - Teucrium montanum. Verbascoside and echinacoside, identified and quantified using LC-MS/MS and HPLC-PAD, were found to be the predominant phenolics in all extracts. Infusion (30 degrees C, 30 min) was characterized with the highest total phenolic content and antioxidant capacity and was further used for encapsulation into liposomes. Formulation of liposomes with a high encapsulation efficiency of echinacoside (68.27%) and verbascoside (80.60%), satisfactory physical properties, including size (326.2 nm) and polydispersity index (0.34), was achieved, although determined zeta potential (-23.03 mV) indicated their instability. Formulated liposomes were successfully coated with pectin and alginate that was also proved by FTIR analysis. Liposomes coated with pectin showed the most desirable in vitro digestion release of verbascoside and echinacoside, while alginate as liposome surface layer proved to be more appropriate for their retention during storage time.
PB  - Elsevier Ltd.
T2  - Food Chemistry
T1  - Formulation and characterization of liposomal encapsulated systems of bioactive ingredients from traditional plant mountain germander (Teucrium montanum L.) for the incorporation into coffee drinks
SP  - 131257
VL  - 370
DO  - 10.1016/j.foodchem.2021.131257
ER  - 

@article{
author = "Šeremet, Danijela and Vugrinec, Kristina and Petrović, Predrag and Butorac, Ana and Kuzmić, Sunčica and Vojvodić Cebin, Aleksandra and Mandura, Ana and Lovrić, Marija and Pjanović, Rada and Komes, Draženka",
year = "2022",
abstract = "Conventional and innovative (microwave-assisted and subcritical water extraction) techniques were applied to investigate the bioactive content of traditional plant - Teucrium montanum. Verbascoside and echinacoside, identified and quantified using LC-MS/MS and HPLC-PAD, were found to be the predominant phenolics in all extracts. Infusion (30 degrees C, 30 min) was characterized with the highest total phenolic content and antioxidant capacity and was further used for encapsulation into liposomes. Formulation of liposomes with a high encapsulation efficiency of echinacoside (68.27%) and verbascoside (80.60%), satisfactory physical properties, including size (326.2 nm) and polydispersity index (0.34), was achieved, although determined zeta potential (-23.03 mV) indicated their instability. Formulated liposomes were successfully coated with pectin and alginate that was also proved by FTIR analysis. Liposomes coated with pectin showed the most desirable in vitro digestion release of verbascoside and echinacoside, while alginate as liposome surface layer proved to be more appropriate for their retention during storage time.",
publisher = "Elsevier Ltd.",
journal = "Food Chemistry",
title = "Formulation and characterization of liposomal encapsulated systems of bioactive ingredients from traditional plant mountain germander (Teucrium montanum L.) for the incorporation into coffee drinks",
pages = "131257",
volume = "370",
doi = "10.1016/j.foodchem.2021.131257"
}

Šeremet, D., Vugrinec, K., Petrović, P., Butorac, A., Kuzmić, S., Vojvodić Cebin, A., Mandura, A., Lovrić, M., Pjanović, R.,& Komes, D.. (2022). Formulation and characterization of liposomal encapsulated systems of bioactive ingredients from traditional plant mountain germander (Teucrium montanum L.) for the incorporation into coffee drinks. in Food Chemistry
Elsevier Ltd.., 370, 131257.
https://doi.org/10.1016/j.foodchem.2021.131257

Šeremet D, Vugrinec K, Petrović P, Butorac A, Kuzmić S, Vojvodić Cebin A, Mandura A, Lovrić M, Pjanović R, Komes D. Formulation and characterization of liposomal encapsulated systems of bioactive ingredients from traditional plant mountain germander (Teucrium montanum L.) for the incorporation into coffee drinks. in Food Chemistry. 2022;370:131257.
doi:10.1016/j.foodchem.2021.131257 .

Šeremet, Danijela, Vugrinec, Kristina, Petrović, Predrag, Butorac, Ana, Kuzmić, Sunčica, Vojvodić Cebin, Aleksandra, Mandura, Ana, Lovrić, Marija, Pjanović, Rada, Komes, Draženka, "Formulation and characterization of liposomal encapsulated systems of bioactive ingredients from traditional plant mountain germander (Teucrium montanum L.) for the incorporation into coffee drinks" in Food Chemistry, 370 (2022):131257,
https://doi.org/10.1016/j.foodchem.2021.131257 . .

TY  - JOUR
AU  - Šeremet, Danijela
AU  - Štefančić, Martina
AU  - Petrović, Predrag
AU  - Kuzmić, Sunčica
AU  - Doroci, Shefkije
AU  - Mandura Jarić, Ana
AU  - Vojvodić Cebin, Aleksandra
AU  - Pjanović, Rada
AU  - Komes, Draženka
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5327
AB  - Ground ivy (Glechoma hederacea L.) has been known as a medicinal plant in folk medicine for generations and, as a member of the Lamiaceae family, is characterized with a high content of rosmarinic acid. The aim of the present study was to formulate delivery systems containing bioac-tive compounds from ground ivy in encapsulated form and incorporated into candies. Liposomes were examined as the encapsulation systems that were additionally coated with an alginate–plant protein gel to reduce leakage of the incorporated material. Bioactive characterization of the ground ivy extract showed a high content of total phenolics (1186.20 mg GAE/L) and rosmarinic acid (46.04 mg/L). The formulation of liposomes with the high encapsulation efficiency of rosmarinic acid (97.64%), with at least a double bilayer and with polydisperse particle size distribution was achieved. Alginate microparticles reinforced with rice proteins provided the highest encapsulation efficiency for rosmarinic acid (78.16%) and were therefore used for the successful coating of lipo-somes, as confirmed by FT‐IR analysis. Coating liposomes with alginate–rice protein gel provided prolonged controlled release of rosmarinic acid during simulated gastro‐intestinal digestion, and the same was noted when they were incorporated into candies.
PB  - MDPI
T2  - Foods
T1  - Development, Characterization and Incorporation of Alginate‐Plant Protein Covered Liposomes Containing Ground Ivy (Glechoma hederacea L.) Extract into Candies
IS  - 12
SP  - 1816
VL  - 11
DO  - 10.3390/foods11121816
ER  - 

@article{
author = "Šeremet, Danijela and Štefančić, Martina and Petrović, Predrag and Kuzmić, Sunčica and Doroci, Shefkije and Mandura Jarić, Ana and Vojvodić Cebin, Aleksandra and Pjanović, Rada and Komes, Draženka",
year = "2022",
abstract = "Ground ivy (Glechoma hederacea L.) has been known as a medicinal plant in folk medicine for generations and, as a member of the Lamiaceae family, is characterized with a high content of rosmarinic acid. The aim of the present study was to formulate delivery systems containing bioac-tive compounds from ground ivy in encapsulated form and incorporated into candies. Liposomes were examined as the encapsulation systems that were additionally coated with an alginate–plant protein gel to reduce leakage of the incorporated material. Bioactive characterization of the ground ivy extract showed a high content of total phenolics (1186.20 mg GAE/L) and rosmarinic acid (46.04 mg/L). The formulation of liposomes with the high encapsulation efficiency of rosmarinic acid (97.64%), with at least a double bilayer and with polydisperse particle size distribution was achieved. Alginate microparticles reinforced with rice proteins provided the highest encapsulation efficiency for rosmarinic acid (78.16%) and were therefore used for the successful coating of lipo-somes, as confirmed by FT‐IR analysis. Coating liposomes with alginate–rice protein gel provided prolonged controlled release of rosmarinic acid during simulated gastro‐intestinal digestion, and the same was noted when they were incorporated into candies.",
publisher = "MDPI",
journal = "Foods",
title = "Development, Characterization and Incorporation of Alginate‐Plant Protein Covered Liposomes Containing Ground Ivy (Glechoma hederacea L.) Extract into Candies",
number = "12",
pages = "1816",
volume = "11",
doi = "10.3390/foods11121816"
}

Šeremet, D., Štefančić, M., Petrović, P., Kuzmić, S., Doroci, S., Mandura Jarić, A., Vojvodić Cebin, A., Pjanović, R.,& Komes, D.. (2022). Development, Characterization and Incorporation of Alginate‐Plant Protein Covered Liposomes Containing Ground Ivy (Glechoma hederacea L.) Extract into Candies. in Foods
MDPI., 11(12), 1816.
https://doi.org/10.3390/foods11121816

Šeremet D, Štefančić M, Petrović P, Kuzmić S, Doroci S, Mandura Jarić A, Vojvodić Cebin A, Pjanović R, Komes D. Development, Characterization and Incorporation of Alginate‐Plant Protein Covered Liposomes Containing Ground Ivy (Glechoma hederacea L.) Extract into Candies. in Foods. 2022;11(12):1816.
doi:10.3390/foods11121816 .

Šeremet, Danijela, Štefančić, Martina, Petrović, Predrag, Kuzmić, Sunčica, Doroci, Shefkije, Mandura Jarić, Ana, Vojvodić Cebin, Aleksandra, Pjanović, Rada, Komes, Draženka, "Development, Characterization and Incorporation of Alginate‐Plant Protein Covered Liposomes Containing Ground Ivy (Glechoma hederacea L.) Extract into Candies" in Foods, 11, no. 12 (2022):1816,
https://doi.org/10.3390/foods11121816 . .

TY  - CONF
AU  - Petrov Ivanković, Anja
AU  - Veljković, Milica
AU  - Vukoičić, Ana
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5994
AB  - According to experts, berries are a major source of phytochemicals, disease-fighting
components, which can help to boost the immune system and lower the risk of many agingrelated conditions. The simplest way to isolate these compounds is through solvent
extraction. However, this method is not entirely successful since, in addition to free
phytochemicals, there are also significant amounts of bound components that are trapped in
the cell wall. For these reasons, the addition of enzymes such as cellulases and pectinases
provides the hydrolysis of the cell wall, facilitate the release of molecules and increase the
extraction yield of phytochemical. Therefore, in this work, enzyme-assisted extraction of
lyophilized blackcurrant (LCR), a fruit that is increasingly used for pharmaceutical and
cosmetic purposes, was examined. Extraction was performed in acetate buffer pH 4.5, at 50 ̊
C using four enzymes: Pectinex® Ultra SP-L, Viscozyme® L, Cellic® CTec3, Rohapect® MC. In
the first step of the experiment, the extraction was optimized by different-time varying, from
10 to 120 min, and after choosing the optimal time, the influence of the solid-liquid ratio (1:4,
1:10, 1:40) on the extraction efficiency was examined. The progress of extraction was
monitored spectrophotometrically, through antioxidant properties, the total content of
polyphenols, flavonoids, and phenolic acids from all blackcurrant extracts and compared to
the control (extract without enzyme). Regarding the total content of polyphenols, it was
revealed that 60 min was the optimal extraction time. On the other hand, it was found that for a solid-liquid ratio of 1:4, all results were about 1.5 times higher than the control values.
In terms of enzymes, Viscozyme® and Pectinex® Ultra SP-L enabled the recovery of the highest
total phenolic content (~28 mg GAE/g LCR) and phenolic acids (~3 mg CA/g LCR), while, on the
other hand, Rohapect® MC enhanced the extraction of antioxidants (FRAP 380 µmol Fe2+/g
LCR), as well as total flavonoids (2.85 mg QE/g LCR). Obtained results open new possibilities
for blackcurrant extracts treated with different enzymes as value-added ingredients for the
cosmetic and food industry
PB  - Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia
C3  - 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts
T1  - Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum)
EP  - 146
SP  - 145
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5994
ER  - 

@conference{
author = "Petrov Ivanković, Anja and Veljković, Milica and Vukoičić, Ana and Milivojević, Ana and Ćorović, Marija and Pjanović, Rada and Bezbradica, Dejan",
year = "2022",
abstract = "According to experts, berries are a major source of phytochemicals, disease-fighting
components, which can help to boost the immune system and lower the risk of many agingrelated conditions. The simplest way to isolate these compounds is through solvent
extraction. However, this method is not entirely successful since, in addition to free
phytochemicals, there are also significant amounts of bound components that are trapped in
the cell wall. For these reasons, the addition of enzymes such as cellulases and pectinases
provides the hydrolysis of the cell wall, facilitate the release of molecules and increase the
extraction yield of phytochemical. Therefore, in this work, enzyme-assisted extraction of
lyophilized blackcurrant (LCR), a fruit that is increasingly used for pharmaceutical and
cosmetic purposes, was examined. Extraction was performed in acetate buffer pH 4.5, at 50 ̊
C using four enzymes: Pectinex® Ultra SP-L, Viscozyme® L, Cellic® CTec3, Rohapect® MC. In
the first step of the experiment, the extraction was optimized by different-time varying, from
10 to 120 min, and after choosing the optimal time, the influence of the solid-liquid ratio (1:4,
1:10, 1:40) on the extraction efficiency was examined. The progress of extraction was
monitored spectrophotometrically, through antioxidant properties, the total content of
polyphenols, flavonoids, and phenolic acids from all blackcurrant extracts and compared to
the control (extract without enzyme). Regarding the total content of polyphenols, it was
revealed that 60 min was the optimal extraction time. On the other hand, it was found that for a solid-liquid ratio of 1:4, all results were about 1.5 times higher than the control values.
In terms of enzymes, Viscozyme® and Pectinex® Ultra SP-L enabled the recovery of the highest
total phenolic content (~28 mg GAE/g LCR) and phenolic acids (~3 mg CA/g LCR), while, on the
other hand, Rohapect® MC enhanced the extraction of antioxidants (FRAP 380 µmol Fe2+/g
LCR), as well as total flavonoids (2.85 mg QE/g LCR). Obtained results open new possibilities
for blackcurrant extracts treated with different enzymes as value-added ingredients for the
cosmetic and food industry",
publisher = "Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia",
journal = "10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts",
title = "Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum)",
pages = "146-145",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5994"
}

Petrov Ivanković, A., Veljković, M., Vukoičić, A., Milivojević, A., Ćorović, M., Pjanović, R.,& Bezbradica, D.. (2022). Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum). in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts
Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia., 145-146.
https://hdl.handle.net/21.15107/rcub_technorep_5994

Petrov Ivanković A, Veljković M, Vukoičić A, Milivojević A, Ćorović M, Pjanović R, Bezbradica D. Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum). in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts. 2022;:145-146.
https://hdl.handle.net/21.15107/rcub_technorep_5994 .

Petrov Ivanković, Anja, Veljković, Milica, Vukoičić, Ana, Milivojević, Ana, Ćorović, Marija, Pjanović, Rada, Bezbradica, Dejan, "Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum)" in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts (2022):145-146,
https://hdl.handle.net/21.15107/rcub_technorep_5994 .

TY  - JOUR
AU  - Petrov, Anja
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5141
AB  - Recently, prebiotics are attracting plenty of attention in the field of skin care, since it is found that they are able to support the balance of beneficial and harmful microorganisms on the skin, and accordingly prevent several skin conditions associated with microbial imbalance. Topical application of prebiotics, although insufficiently investigated, holds great promise in improving skin health. The purpose of this research was to determine the prebiotic potential of galacto-oligosaccharides (GOS) for skin microbiota and suitability for incorporation in different topical formulations, and finally, provide insights into their diffusion properties. Methods: The prebiotic effect of GOS was evaluated through the influence on the growth of Staphylococcus epidermidis and Staphylococcus aureus, the most common resident and pathogenic bacterium of the skin microbiota, respectively. Also, with the future use of GOS in cosmetic products in mind, the diffusion of GOS molecules from two different topical formulations, hydrogel and oil-in-water (O/W) gel emulsion, was monitored employing Franz diffusion cell and two systems—with cellulose acetate membrane and transdermal diffusion test model, Strat-M® membrane. Course of fermentation and the amount of diffused GOS molecules were monitored using high-performance liquid chromatography (HPLC). Results: The in vitro results revealed that GOS at a concentration of 5% (w/v) has a pronounced stimulatory effect on S. epidermidis, while simultaneously showing an inhibitory effect on S. aureus, both in nutrient broth and cosmetic formulations. GOS trisaccharide and tetrasaccharide diffusion coefficients from O/W gel emulsion were calculated to be 5.61·10−6 cm2 s−1 and 1.41·10−8 cm2 s−1, respectively. The diffusion coefficient of GOS trisaccharides from hydrogel was 3.22·10−6 cm2 s−1, while it was not determined for tetrasaccharides due to low diffused concentration. Transdermal diffusion tests revealed that GOS incorporated in two formulations stays at the surface of the skin even after 24 h. Conclusion: When applied in adequate concentration, GOS has the potential to be used as a skin prebiotic. Novel GOS enriched formulations, Aristoflex® AVC-based hydrogel and Heliogel™-based O/W gel emulsion, provided efficient diffusion and delivery of prebiotic GOS molecules to the skin surface.
PB  - John Wiley and Sons Inc.
T2  - International Journal of Cosmetic Science
T1  - Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties
EP  - 319
IS  - 3
SP  - 309
VL  - 44
DO  - 10.1111/ics.12778
ER  - 

@article{
author = "Petrov, Anja and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Banjanac, Katarina and Pjanović, Rada and Bezbradica, Dejan",
year = "2022",
abstract = "Recently, prebiotics are attracting plenty of attention in the field of skin care, since it is found that they are able to support the balance of beneficial and harmful microorganisms on the skin, and accordingly prevent several skin conditions associated with microbial imbalance. Topical application of prebiotics, although insufficiently investigated, holds great promise in improving skin health. The purpose of this research was to determine the prebiotic potential of galacto-oligosaccharides (GOS) for skin microbiota and suitability for incorporation in different topical formulations, and finally, provide insights into their diffusion properties. Methods: The prebiotic effect of GOS was evaluated through the influence on the growth of Staphylococcus epidermidis and Staphylococcus aureus, the most common resident and pathogenic bacterium of the skin microbiota, respectively. Also, with the future use of GOS in cosmetic products in mind, the diffusion of GOS molecules from two different topical formulations, hydrogel and oil-in-water (O/W) gel emulsion, was monitored employing Franz diffusion cell and two systems—with cellulose acetate membrane and transdermal diffusion test model, Strat-M® membrane. Course of fermentation and the amount of diffused GOS molecules were monitored using high-performance liquid chromatography (HPLC). Results: The in vitro results revealed that GOS at a concentration of 5% (w/v) has a pronounced stimulatory effect on S. epidermidis, while simultaneously showing an inhibitory effect on S. aureus, both in nutrient broth and cosmetic formulations. GOS trisaccharide and tetrasaccharide diffusion coefficients from O/W gel emulsion were calculated to be 5.61·10−6 cm2 s−1 and 1.41·10−8 cm2 s−1, respectively. The diffusion coefficient of GOS trisaccharides from hydrogel was 3.22·10−6 cm2 s−1, while it was not determined for tetrasaccharides due to low diffused concentration. Transdermal diffusion tests revealed that GOS incorporated in two formulations stays at the surface of the skin even after 24 h. Conclusion: When applied in adequate concentration, GOS has the potential to be used as a skin prebiotic. Novel GOS enriched formulations, Aristoflex® AVC-based hydrogel and Heliogel™-based O/W gel emulsion, provided efficient diffusion and delivery of prebiotic GOS molecules to the skin surface.",
publisher = "John Wiley and Sons Inc.",
journal = "International Journal of Cosmetic Science",
title = "Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties",
pages = "319-309",
number = "3",
volume = "44",
doi = "10.1111/ics.12778"
}

Petrov, A., Ćorović, M., Milivojević, A., Simović, M., Banjanac, K., Pjanović, R.,& Bezbradica, D.. (2022). Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties. in International Journal of Cosmetic Science
John Wiley and Sons Inc.., 44(3), 309-319.
https://doi.org/10.1111/ics.12778

Petrov A, Ćorović M, Milivojević A, Simović M, Banjanac K, Pjanović R, Bezbradica D. Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties. in International Journal of Cosmetic Science. 2022;44(3):309-319.
doi:10.1111/ics.12778 .

Petrov, Anja, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Banjanac, Katarina, Pjanović, Rada, Bezbradica, Dejan, "Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties" in International Journal of Cosmetic Science, 44, no. 3 (2022):309-319,
https://doi.org/10.1111/ics.12778 . .

TY  - JOUR
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5203
AB  - This work aims to evaluate the effect of cosmetic formulations on the skin permeation of phloridzin acetates, therefore in vitro transdermal delivery of these bioactive compounds across Strat-M membrane is investigated. The formulations tested are gel emulsion, oil in water emulsion (O/W emulsion), gel in oil emulsion, liquid crystal emulsion, and liposomes dispersed in gel. The release of esters from the O/W emulsion is diffusion-controlled (n = 0.5), while release kinetics from gel-based formulations depends on coupled diffusion and polymer relaxations effect (0.5 < n < 1). The highest values of effective diffusion coefficient are obtained after release from O/W emulsion (24.64 × 10−10 m2 min−1 for phloridzin acetate and 6.55 × 10−10 m2 min−1 for phloridzin diacetate). The lag phase lasting from 1 h (gel emulsion) to 5 h (liquid crystal emulsion) indicates that esters permeation is significantly affected by the formulation used. Formulations in which the water is continuous phase are the most appropriate carrier system for transdermal delivery of phloridzin esters. Additionally, liposomes are successfully applied as carrier systems for the controlled release of esters, although it should be noted that liposomes without cholesterol show remarkable stability (ζ = −43 mV) and homogeneity (polydispersity index = 0.198). Practical application: Phloridzin is a flavonoid glycoside that possesses many beneficial activities such as antioxidant, UV-protecting, and anti-inflammatory activity, so the use of this flavonoid and its derivatives in numerous cosmetic and pharmaceutical formulations seems very attractive. Results obtained in this study give a comprehensive insight into the release mechanism of phloridzin acetates from emulsions and liposomes, enable the selection of optimum cosmetic formulations depending on the application, and provide significant scientific contribution in the field of topical administration of bioactive flavonoid derivatives.
PB  - John Wiley and Sons Inc.
T2  - European Journal of Lipid Science and Technology
T1  - Evaluation of In Vitro Skin Permeation of Enzymatically Synthesized Phloridzin Acetates from Emulsions and Liposomes Dispersed in Gel
IS  - 10
SP  - 2200073
VL  - 124
DO  - 10.1002/ejlt.202200073
ER  - 

@article{
author = "Milivojević, Ana and Ćorović, Marija and Simović, Milica and Banjanac, Katarina and Pjanović, Rada and Bezbradica, Dejan",
year = "2022",
abstract = "This work aims to evaluate the effect of cosmetic formulations on the skin permeation of phloridzin acetates, therefore in vitro transdermal delivery of these bioactive compounds across Strat-M membrane is investigated. The formulations tested are gel emulsion, oil in water emulsion (O/W emulsion), gel in oil emulsion, liquid crystal emulsion, and liposomes dispersed in gel. The release of esters from the O/W emulsion is diffusion-controlled (n = 0.5), while release kinetics from gel-based formulations depends on coupled diffusion and polymer relaxations effect (0.5 < n < 1). The highest values of effective diffusion coefficient are obtained after release from O/W emulsion (24.64 × 10−10 m2 min−1 for phloridzin acetate and 6.55 × 10−10 m2 min−1 for phloridzin diacetate). The lag phase lasting from 1 h (gel emulsion) to 5 h (liquid crystal emulsion) indicates that esters permeation is significantly affected by the formulation used. Formulations in which the water is continuous phase are the most appropriate carrier system for transdermal delivery of phloridzin esters. Additionally, liposomes are successfully applied as carrier systems for the controlled release of esters, although it should be noted that liposomes without cholesterol show remarkable stability (ζ = −43 mV) and homogeneity (polydispersity index = 0.198). Practical application: Phloridzin is a flavonoid glycoside that possesses many beneficial activities such as antioxidant, UV-protecting, and anti-inflammatory activity, so the use of this flavonoid and its derivatives in numerous cosmetic and pharmaceutical formulations seems very attractive. Results obtained in this study give a comprehensive insight into the release mechanism of phloridzin acetates from emulsions and liposomes, enable the selection of optimum cosmetic formulations depending on the application, and provide significant scientific contribution in the field of topical administration of bioactive flavonoid derivatives.",
publisher = "John Wiley and Sons Inc.",
journal = "European Journal of Lipid Science and Technology",
title = "Evaluation of In Vitro Skin Permeation of Enzymatically Synthesized Phloridzin Acetates from Emulsions and Liposomes Dispersed in Gel",
number = "10",
pages = "2200073",
volume = "124",
doi = "10.1002/ejlt.202200073"
}

Milivojević, A., Ćorović, M., Simović, M., Banjanac, K., Pjanović, R.,& Bezbradica, D.. (2022). Evaluation of In Vitro Skin Permeation of Enzymatically Synthesized Phloridzin Acetates from Emulsions and Liposomes Dispersed in Gel. in European Journal of Lipid Science and Technology
John Wiley and Sons Inc.., 124(10), 2200073.
https://doi.org/10.1002/ejlt.202200073

Milivojević A, Ćorović M, Simović M, Banjanac K, Pjanović R, Bezbradica D. Evaluation of In Vitro Skin Permeation of Enzymatically Synthesized Phloridzin Acetates from Emulsions and Liposomes Dispersed in Gel. in European Journal of Lipid Science and Technology. 2022;124(10):2200073.
doi:10.1002/ejlt.202200073 .

Milivojević, Ana, Ćorović, Marija, Simović, Milica, Banjanac, Katarina, Pjanović, Rada, Bezbradica, Dejan, "Evaluation of In Vitro Skin Permeation of Enzymatically Synthesized Phloridzin Acetates from Emulsions and Liposomes Dispersed in Gel" in European Journal of Lipid Science and Technology, 124, no. 10 (2022):2200073,
https://doi.org/10.1002/ejlt.202200073 . .

TY  - JOUR
AU  - Jaćimovski, Darko
AU  - Brzić, Danica
AU  - Garić-Grulović, Radmila
AU  - Pjanović, Rada
AU  - Đuriš, Mihal
AU  - Arsenijević, Zorana
AU  - Bošković-Vragolović, Nevenka
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5064
AB  - In this work the theoretical model for heat transfer from a wall to a
liquid-solid fluidized bed by liquid convective mechanism has been proposed.
The model is based on thickness of boundary layer and film theory. The key
parameter in the model is the distance between two adjacent particles which
collide with the wall. According to the proposed model, the liquid convective
heat transfer in a fluidized bed is 4 to 5 times more intense than in a single-phase
flow. Additionally, the wall-to-bed heat transfer coefficient has been measured
experimentally in water –glass particles fluidized bed, for different particle sizes.
Comparison of the model prediction with experimental data has shown that size
of the particles strongly influences the mechanism of heat transfer. For fine
particles of 0.8 mm in diameter, the liquid convective heat transfer model
represents adequately the experimental data, indicating that particle convective
mechanism is negligible. For coarse particles of 1.5 - 2 mm in diameter, the
liquid convective heat transfer mechanism accounts for 60 % of the overall heat
transfer coefficient
AB  - У овом раду развијен је теоријски модел преноса топлоте конвективним механизмом
са зида колоне на течност у партикулативно флуидизованом слоју. Модел је заснован на
дебљини граничног слоја и теорији филма. Кључни параметар модела је растојање између
двe суседне честице које се сударају са зидом колоне. Према предложеном моделу пренос
топлоте конвекцијом на течност у флуидизованом слоју је 4 до 5 пута интензивнији него у
једнофазном току. Коефицијенти преноса топлоте су експериментално одређени у
флуидизованом слоју вода-стаклене сфере, за различите пречнике честица. Поређење
експерименталних резултата и предвиђања модела је показало да пречник честица значајно
утиче на механизам преноса топлоте. За веома ситне честице пречника 0.8 mm, модел
преноса топлоте конвекцијом на течност у потпуности репрезентује експерименталне
резултате, указујући да је пренос топлоте честицама занемарљив. За крупне честице,
пречника 1.5 -2 mm, конвективни пренос на течност чини 60 % од укупног коефицијента
преноса топлоте
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Heat transfer by liquid convection in particulate fluidized beds
T1  - ПРЕНОС ТОПЛОТЕ КОНВЕКТИВНИМ МЕХАНИЗМОМ У ПАРТИКУЛАТИВНО ФЛУИДИЗОВАНОМ СЛОЈУ
DO  - 10.2298/JSC211216020J
ER  - 

@article{
author = "Jaćimovski, Darko and Brzić, Danica and Garić-Grulović, Radmila and Pjanović, Rada and Đuriš, Mihal and Arsenijević, Zorana and Bošković-Vragolović, Nevenka",
year = "2022",
abstract = "In this work the theoretical model for heat transfer from a wall to a
liquid-solid fluidized bed by liquid convective mechanism has been proposed.
The model is based on thickness of boundary layer and film theory. The key
parameter in the model is the distance between two adjacent particles which
collide with the wall. According to the proposed model, the liquid convective
heat transfer in a fluidized bed is 4 to 5 times more intense than in a single-phase
flow. Additionally, the wall-to-bed heat transfer coefficient has been measured
experimentally in water –glass particles fluidized bed, for different particle sizes.
Comparison of the model prediction with experimental data has shown that size
of the particles strongly influences the mechanism of heat transfer. For fine
particles of 0.8 mm in diameter, the liquid convective heat transfer model
represents adequately the experimental data, indicating that particle convective
mechanism is negligible. For coarse particles of 1.5 - 2 mm in diameter, the
liquid convective heat transfer mechanism accounts for 60 % of the overall heat
transfer coefficient, У овом раду развијен је теоријски модел преноса топлоте конвективним механизмом
са зида колоне на течност у партикулативно флуидизованом слоју. Модел је заснован на
дебљини граничног слоја и теорији филма. Кључни параметар модела је растојање између
двe суседне честице које се сударају са зидом колоне. Према предложеном моделу пренос
топлоте конвекцијом на течност у флуидизованом слоју је 4 до 5 пута интензивнији него у
једнофазном току. Коефицијенти преноса топлоте су експериментално одређени у
флуидизованом слоју вода-стаклене сфере, за различите пречнике честица. Поређење
експерименталних резултата и предвиђања модела је показало да пречник честица значајно
утиче на механизам преноса топлоте. За веома ситне честице пречника 0.8 mm, модел
преноса топлоте конвекцијом на течност у потпуности репрезентује експерименталне
резултате, указујући да је пренос топлоте честицама занемарљив. За крупне честице,
пречника 1.5 -2 mm, конвективни пренос на течност чини 60 % од укупног коефицијента
преноса топлоте",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Heat transfer by liquid convection in particulate fluidized beds, ПРЕНОС ТОПЛОТЕ КОНВЕКТИВНИМ МЕХАНИЗМОМ У ПАРТИКУЛАТИВНО ФЛУИДИЗОВАНОМ СЛОЈУ",
doi = "10.2298/JSC211216020J"
}

Jaćimovski, D., Brzić, D., Garić-Grulović, R., Pjanović, R., Đuriš, M., Arsenijević, Z.,& Bošković-Vragolović, N.. (2022). Heat transfer by liquid convection in particulate fluidized beds. in Journal of the Serbian Chemical Society
Serbian Chemical Society..
https://doi.org/10.2298/JSC211216020J

Jaćimovski D, Brzić D, Garić-Grulović R, Pjanović R, Đuriš M, Arsenijević Z, Bošković-Vragolović N. Heat transfer by liquid convection in particulate fluidized beds. in Journal of the Serbian Chemical Society. 2022;.
doi:10.2298/JSC211216020J .

Jaćimovski, Darko, Brzić, Danica, Garić-Grulović, Radmila, Pjanović, Rada, Đuriš, Mihal, Arsenijević, Zorana, Bošković-Vragolović, Nevenka, "Heat transfer by liquid convection in particulate fluidized beds" in Journal of the Serbian Chemical Society (2022),
https://doi.org/10.2298/JSC211216020J . .

TY  - JOUR
AU  - Jaćimovski, Darko R.
AU  - Brzić, Danica V.
AU  - Garić-Grulović, Radmila V.
AU  - Pjanović, Rada V.
AU  - Đuriš, Mihal M.
AU  - Arsenijević, Zorana L.J.
AU  - Bošković-Vragolović, Nevenka M.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5231
AB  - In this work the theoretical model for heat transfer from a wall to a liquid-solid fluidized bed by liquid convective mechanism has been proposed and developed. The model is based on thickness of boundary layer and film theory. The key parameter in the model is the distance between two adjacent particles which collide with the wall. According to the proposed model, the liquid convective heat transfer in a fluidized bed is 4 to 5 times more intense than in a single-phase flow. Additionally, the wall-to-bed heat transfer coefficient has been measured experimentally in water–glass particles fluidized bed, for different particle sizes. Comparison of the model prediction with experimental data has shown that size of the particles strongly influences the mechanism of heat transfer. For fine particles of 0.8 mm in diameter, the liquid convective heat transfer model represents adequately the experimental data, indicating that particle convective mechanism is negligible. For coarse particles of 1.5–2 mm in diameter, the liquid convective heat transfer mechanism accounts for 60 % of the overall heat transfer coefficient.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Heat transfer by liquid convection in particulate fluidized beds
T1  - ПРЕНОС ТОПЛОТЕ КОНВЕКТИВНИМ МЕХАНИЗМОМ У ПАРТИКУЛАТИВНО ФЛУИДИЗОВАНОМ СЛОЈУ
EP  - 924
IS  - 7-8
SP  - 911
VL  - 87
DO  - 10.2298/JSC211216020J
ER  - 

@article{
author = "Jaćimovski, Darko R. and Brzić, Danica V. and Garić-Grulović, Radmila V. and Pjanović, Rada V. and Đuriš, Mihal M. and Arsenijević, Zorana L.J. and Bošković-Vragolović, Nevenka M.",
year = "2022",
abstract = "In this work the theoretical model for heat transfer from a wall to a liquid-solid fluidized bed by liquid convective mechanism has been proposed and developed. The model is based on thickness of boundary layer and film theory. The key parameter in the model is the distance between two adjacent particles which collide with the wall. According to the proposed model, the liquid convective heat transfer in a fluidized bed is 4 to 5 times more intense than in a single-phase flow. Additionally, the wall-to-bed heat transfer coefficient has been measured experimentally in water–glass particles fluidized bed, for different particle sizes. Comparison of the model prediction with experimental data has shown that size of the particles strongly influences the mechanism of heat transfer. For fine particles of 0.8 mm in diameter, the liquid convective heat transfer model represents adequately the experimental data, indicating that particle convective mechanism is negligible. For coarse particles of 1.5–2 mm in diameter, the liquid convective heat transfer mechanism accounts for 60 % of the overall heat transfer coefficient.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Heat transfer by liquid convection in particulate fluidized beds, ПРЕНОС ТОПЛОТЕ КОНВЕКТИВНИМ МЕХАНИЗМОМ У ПАРТИКУЛАТИВНО ФЛУИДИЗОВАНОМ СЛОЈУ",
pages = "924-911",
number = "7-8",
volume = "87",
doi = "10.2298/JSC211216020J"
}

Jaćimovski, D. R., Brzić, D. V., Garić-Grulović, R. V., Pjanović, R. V., Đuriš, M. M., Arsenijević, Z. L.J.,& Bošković-Vragolović, N. M.. (2022). Heat transfer by liquid convection in particulate fluidized beds. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 87(7-8), 911-924.
https://doi.org/10.2298/JSC211216020J

Jaćimovski DR, Brzić DV, Garić-Grulović RV, Pjanović RV, Đuriš MM, Arsenijević ZL, Bošković-Vragolović NM. Heat transfer by liquid convection in particulate fluidized beds. in Journal of the Serbian Chemical Society. 2022;87(7-8):911-924.
doi:10.2298/JSC211216020J .

Jaćimovski, Darko R., Brzić, Danica V., Garić-Grulović, Radmila V., Pjanović, Rada V., Đuriš, Mihal M., Arsenijević, Zorana L.J., Bošković-Vragolović, Nevenka M., "Heat transfer by liquid convection in particulate fluidized beds" in Journal of the Serbian Chemical Society, 87, no. 7-8 (2022):911-924,
https://doi.org/10.2298/JSC211216020J . .

TY  - CONF
AU  - Marković, Maja D.
AU  - Panić, Vesna V.
AU  - Tadic, Julijana D.
AU  - Pjanović, Rada V.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5069
AB  - Targeted drug delivery is powerful tool which researchers use to achieve safer and more efficient
therapy of many diseases, including various types of cancer. Many chemotherapeutics are poorly watersoluble, so their encapsulation and targeted delivery remain quite challenge. Hydrogels based on
poly(methacrylic acid) (PMAA) are widely investigated for targeted drug delivery due to their pH
sensitivity, non-toxicity and biocompatibility. Still, due to the PMAA highly hydrophilic nature, PMAA
can only be used for encapsulation and targeted delivery of water-soluble drugs. Our previous research
was directed towards overcoming this limitation: PMAA was modified with amphiphilic protein –
casein and poorly-water soluble model drug – caffeine – was encapsulated (PMAC). Present study is
focused on investigation how variation of amount of one of the most important hydrogels network
parameter such as crosslinker affect PMAC swelling properties and caffeine release. The group of
hybrid hydrogels – PMAC – was synthesized with various amount of crosslinker: 0.4mol%, 0.8mol%,
1.6mol% and 3.2mol% with respect to methacrylic acid. Swelling behavior of hybrid hydrogels and
caffeine release was investigated in two environments which simulated human stomach and intestines.
Obtained results showed that targeted delivery of poorly water-soluble model drug was achieved and
that its release can be prolonged up to 24h. Also, kinetic of poorly water-soluble drug release can be
easily modified only by changing crosslinker amount. PMAC hybrid hydrogels have huge potential for
targeted delivery of poorly water-soluble active substances.
PB  - Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2021
C3  - 1 st International Conference on Chemo and BioInformatics ICCBIKG 2021
T1  - Effect of crosslinker amount on hybrid hydrogels swelling and drug release
EP  - 128
SP  - 125
DO  - 10.46793/ICCBI21.125M
ER  - 

@conference{
author = "Marković, Maja D. and Panić, Vesna V. and Tadic, Julijana D. and Pjanović, Rada V.",
year = "2021",
abstract = "Targeted drug delivery is powerful tool which researchers use to achieve safer and more efficient
therapy of many diseases, including various types of cancer. Many chemotherapeutics are poorly watersoluble, so their encapsulation and targeted delivery remain quite challenge. Hydrogels based on
poly(methacrylic acid) (PMAA) are widely investigated for targeted drug delivery due to their pH
sensitivity, non-toxicity and biocompatibility. Still, due to the PMAA highly hydrophilic nature, PMAA
can only be used for encapsulation and targeted delivery of water-soluble drugs. Our previous research
was directed towards overcoming this limitation: PMAA was modified with amphiphilic protein –
casein and poorly-water soluble model drug – caffeine – was encapsulated (PMAC). Present study is
focused on investigation how variation of amount of one of the most important hydrogels network
parameter such as crosslinker affect PMAC swelling properties and caffeine release. The group of
hybrid hydrogels – PMAC – was synthesized with various amount of crosslinker: 0.4mol%, 0.8mol%,
1.6mol% and 3.2mol% with respect to methacrylic acid. Swelling behavior of hybrid hydrogels and
caffeine release was investigated in two environments which simulated human stomach and intestines.
Obtained results showed that targeted delivery of poorly water-soluble model drug was achieved and
that its release can be prolonged up to 24h. Also, kinetic of poorly water-soluble drug release can be
easily modified only by changing crosslinker amount. PMAC hybrid hydrogels have huge potential for
targeted delivery of poorly water-soluble active substances.",
publisher = "Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2021",
journal = "1 st International Conference on Chemo and BioInformatics ICCBIKG 2021",
title = "Effect of crosslinker amount on hybrid hydrogels swelling and drug release",
pages = "128-125",
doi = "10.46793/ICCBI21.125M"
}

Marković, M. D., Panić, V. V., Tadic, J. D.,& Pjanović, R. V.. (2021). Effect of crosslinker amount on hybrid hydrogels swelling and drug release. in 1 st International Conference on Chemo and BioInformatics ICCBIKG 2021
Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2021., 125-128.
https://doi.org/10.46793/ICCBI21.125M

Marković MD, Panić VV, Tadic JD, Pjanović RV. Effect of crosslinker amount on hybrid hydrogels swelling and drug release. in 1 st International Conference on Chemo and BioInformatics ICCBIKG 2021. 2021;:125-128.
doi:10.46793/ICCBI21.125M .

Marković, Maja D., Panić, Vesna V., Tadic, Julijana D., Pjanović, Rada V., "Effect of crosslinker amount on hybrid hydrogels swelling and drug release" in 1 st International Conference on Chemo and BioInformatics ICCBIKG 2021 (2021):125-128,
https://doi.org/10.46793/ICCBI21.125M . .

TY  - CONF
AU  - Marković, Maja D.
AU  - Tadic, Julijana D.
AU  - Pjanović, Rada V.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5070
AB  - Nowadays, humanity are faced with many challenges which affect health of people all around
the globe (such as climate change, new diseases and/or already present ones for which cure has
not been found yet – cancer). The efforts of researchers on the field of drug delivery systems
bring everyday novel tools for safer and more effective therapy. pH sensitive hydrogels based
on poly(methacrylic acid) are recognized as materials with huge potential for controlled release
of drugs. The encapsulation and controlled release of many chemotherapeutics is quite
challenge due to their poorly water-solubility. In our previous research we overcome this
problem by modifying hydrophilic pol(methacrylic acid) with amphiphilic casein and showed
that prepared material have potential for encapsulation and controlled release of poorly watersoluble model drug – caffeine (PMAC carriers). In present study we deepened further our
research and employed various models: Ritger-Peppas, Higuchi and Kopcha model to analyze
how the change of crosslinker amount affect the mechanism of release kinetics of caffeine in
medium with pH of 6.8 (which simulated the environment in human intestines). Obtained
results showed that only by changing one parameter such as crosslinker amount it is possible to
fine tune the type of drug release mechanism, due to which the PMAC carriers would be able
to respond to the specific demands of therapy.
PB  - University of Szeged, H-6720 Szeged, Dugonics tér 13, Hungary
C3  - The 27 th International Symposium on Analytical and Environmental Problems
T1  - Analysis of kinetics of poorly water-soluble drug release from hydrogels based on poly(methacrylic acid) and casein with different crosslinker amount
EP  - 207
SP  - 203
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5070
ER  - 

@conference{
author = "Marković, Maja D. and Tadic, Julijana D. and Pjanović, Rada V.",
year = "2021",
abstract = "Nowadays, humanity are faced with many challenges which affect health of people all around
the globe (such as climate change, new diseases and/or already present ones for which cure has
not been found yet – cancer). The efforts of researchers on the field of drug delivery systems
bring everyday novel tools for safer and more effective therapy. pH sensitive hydrogels based
on poly(methacrylic acid) are recognized as materials with huge potential for controlled release
of drugs. The encapsulation and controlled release of many chemotherapeutics is quite
challenge due to their poorly water-solubility. In our previous research we overcome this
problem by modifying hydrophilic pol(methacrylic acid) with amphiphilic casein and showed
that prepared material have potential for encapsulation and controlled release of poorly watersoluble model drug – caffeine (PMAC carriers). In present study we deepened further our
research and employed various models: Ritger-Peppas, Higuchi and Kopcha model to analyze
how the change of crosslinker amount affect the mechanism of release kinetics of caffeine in
medium with pH of 6.8 (which simulated the environment in human intestines). Obtained
results showed that only by changing one parameter such as crosslinker amount it is possible to
fine tune the type of drug release mechanism, due to which the PMAC carriers would be able
to respond to the specific demands of therapy.",
publisher = "University of Szeged, H-6720 Szeged, Dugonics tér 13, Hungary",
journal = "The 27 th International Symposium on Analytical and Environmental Problems",
title = "Analysis of kinetics of poorly water-soluble drug release from hydrogels based on poly(methacrylic acid) and casein with different crosslinker amount",
pages = "207-203",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5070"
}

Marković, M. D., Tadic, J. D.,& Pjanović, R. V.. (2021). Analysis of kinetics of poorly water-soluble drug release from hydrogels based on poly(methacrylic acid) and casein with different crosslinker amount. in The 27 th International Symposium on Analytical and Environmental Problems
University of Szeged, H-6720 Szeged, Dugonics tér 13, Hungary., 203-207.
https://hdl.handle.net/21.15107/rcub_technorep_5070

Marković MD, Tadic JD, Pjanović RV. Analysis of kinetics of poorly water-soluble drug release from hydrogels based on poly(methacrylic acid) and casein with different crosslinker amount. in The 27 th International Symposium on Analytical and Environmental Problems. 2021;:203-207.
https://hdl.handle.net/21.15107/rcub_technorep_5070 .

Marković, Maja D., Tadic, Julijana D., Pjanović, Rada V., "Analysis of kinetics of poorly water-soluble drug release from hydrogels based on poly(methacrylic acid) and casein with different crosslinker amount" in The 27 th International Symposium on Analytical and Environmental Problems (2021):203-207,
https://hdl.handle.net/21.15107/rcub_technorep_5070 .

TY  - JOUR
AU  - Marković, Maja
AU  - Seslija, Sanja, I
AU  - Ugrinović, Vukašin
AU  - Kunaver, Matjaz
AU  - Panić, Vesna
AU  - Pjanović, Rada
AU  - Spasojević, Pavle
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4802
AB  - pH- and magnetic-sensitive hybrid hydrogels based on poly(methacrylic acid) (PMAA), nanocellulose (NC), carboxymethyl cellulose (CMC) and magnetite particles (MN) were prepared as ibuprofen delivery system. NC was isolated from wood waste material and added to highly hydrophilic PMAA in order to improve the mechanical properties of PMAA and enable the encapsulation of poorly water-soluble ibuprofen. CMC was added in order to stabilize magnetite particles. The pH- and magnetic-sensitivity of the obtained hybrid hydrogels (originated from PMAA and MN, respectively) provided targeted delivery and controlled release of ibuprofen. Hybrid hydrogels were characterized by various technics: FTIR, SEM, XRD, VSM, the modified Faraday method, photon correlation spectroscopy and by using single compression tests. The swelling behavior of hybrid hydrogels and cumulative ibuprofen release were investigated depending on wt% of NC and MN in two media which were simulating pH environments in human stomach and intestines. The swelling degree of hybrid hydrogels and the percent of cumulative ibuprofen release decreased with the increase in wt% of NC. The same was found for increasing wt% of MN. Ibuprofen release kinetics was investigated with Korsmeyer-Peppas model. The obtained results showed that the prepared green dual responsive hybrid hydrogels were excellent candidates for the controlled release of ibuprofen, showing that the release kinetic can be easily tuned by changing only one hydrogel component.
T2  - Cellulose
T1  - Green pH- and magnetic-responsive hybrid hydrogels based on poly(methacrylic acid) and Eucalyptus wood nanocellulose for controlled release of ibuprofen
EP  - 11132
IS  - 17
SP  - 11109
VL  - 28
DO  - 10.1007/s10570-021-04222-w
ER  - 

@article{
author = "Marković, Maja and Seslija, Sanja, I and Ugrinović, Vukašin and Kunaver, Matjaz and Panić, Vesna and Pjanović, Rada and Spasojević, Pavle",
year = "2021",
abstract = "pH- and magnetic-sensitive hybrid hydrogels based on poly(methacrylic acid) (PMAA), nanocellulose (NC), carboxymethyl cellulose (CMC) and magnetite particles (MN) were prepared as ibuprofen delivery system. NC was isolated from wood waste material and added to highly hydrophilic PMAA in order to improve the mechanical properties of PMAA and enable the encapsulation of poorly water-soluble ibuprofen. CMC was added in order to stabilize magnetite particles. The pH- and magnetic-sensitivity of the obtained hybrid hydrogels (originated from PMAA and MN, respectively) provided targeted delivery and controlled release of ibuprofen. Hybrid hydrogels were characterized by various technics: FTIR, SEM, XRD, VSM, the modified Faraday method, photon correlation spectroscopy and by using single compression tests. The swelling behavior of hybrid hydrogels and cumulative ibuprofen release were investigated depending on wt% of NC and MN in two media which were simulating pH environments in human stomach and intestines. The swelling degree of hybrid hydrogels and the percent of cumulative ibuprofen release decreased with the increase in wt% of NC. The same was found for increasing wt% of MN. Ibuprofen release kinetics was investigated with Korsmeyer-Peppas model. The obtained results showed that the prepared green dual responsive hybrid hydrogels were excellent candidates for the controlled release of ibuprofen, showing that the release kinetic can be easily tuned by changing only one hydrogel component.",
journal = "Cellulose",
title = "Green pH- and magnetic-responsive hybrid hydrogels based on poly(methacrylic acid) and Eucalyptus wood nanocellulose for controlled release of ibuprofen",
pages = "11132-11109",
number = "17",
volume = "28",
doi = "10.1007/s10570-021-04222-w"
}

Marković, M., Seslija, S. I., Ugrinović, V., Kunaver, M., Panić, V., Pjanović, R.,& Spasojević, P.. (2021). Green pH- and magnetic-responsive hybrid hydrogels based on poly(methacrylic acid) and Eucalyptus wood nanocellulose for controlled release of ibuprofen. in Cellulose, 28(17), 11109-11132.
https://doi.org/10.1007/s10570-021-04222-w

Marković M, Seslija SI, Ugrinović V, Kunaver M, Panić V, Pjanović R, Spasojević P. Green pH- and magnetic-responsive hybrid hydrogels based on poly(methacrylic acid) and Eucalyptus wood nanocellulose for controlled release of ibuprofen. in Cellulose. 2021;28(17):11109-11132.
doi:10.1007/s10570-021-04222-w .

Marković, Maja, Seslija, Sanja, I, Ugrinović, Vukašin, Kunaver, Matjaz, Panić, Vesna, Pjanović, Rada, Spasojević, Pavle, "Green pH- and magnetic-responsive hybrid hydrogels based on poly(methacrylic acid) and Eucalyptus wood nanocellulose for controlled release of ibuprofen" in Cellulose, 28, no. 17 (2021):11109-11132,
https://doi.org/10.1007/s10570-021-04222-w . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Levic, Steva M.
AU  - Pavlović, Vladimir B.
AU  - Markovic, Smilja B.
AU  - Pjanović, Rada
AU  - Đorđević, Verica
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4863
AB  - Freeze drying was compared with spray drying regarding feasibility to process wild thyme drugs in order to obtain dry formulations at laboratory scale starting from liquid extracts produced by different extraction methods: maceration and heat-, ultrasound-, and microwave-assisted extractions. Higher total powder yield (based on the dry weight prior to extraction) was achieved by freeze than spray drying and lower loss of total polyphenol content (TPC) and total flavonoid content (TFC) due to the drying process. Gelatin as a coating agent (5% w/w) provided better TPC recovery by 70% in case of lyophilization and higher total powder yield in case of spray drying by diminishing material deposition on the wall of the drying chamber. The resulting gelatin-free and gelatin-containing powders carried polyphenols in amount similar to 190 and 53-75 mg gallic acid equivalents GAE/g of powder, respectively. Microwave-assisted extract formulation was distinguished from the others by a higher content of polyphenols, proteins and sugars, higher bulk density and lower solubility. The type of the drying process mainly affected the position of the gelatin-derived -OH and amide bands in FTIR spectra. Spray-dried formulations compared to freeze-dried expressed higher thermal stability as confirmed by differential scanning calorimetry analysis and a higher diffusion coefficient; the last feature can be associated with the lower specific surface area of irregularly shaped freeze-dried particles (151-223 mu m) compared to small microspheres (similar to 8 mu m) in spray-dried powder.
T2  - Molecules
T1  - Freeze vs. Spray Drying for Dry Wild Thyme (Thymus serpyllum L.) Extract Formulations: The Impact of Gelatin as a Coating Material
IS  - 13
VL  - 26
DO  - 10.3390/molecules26133933
ER  - 

@article{
author = "Jovanović, Aleksandra and Levic, Steva M. and Pavlović, Vladimir B. and Markovic, Smilja B. and Pjanović, Rada and Đorđević, Verica and Nedović, Viktor and Bugarski, Branko",
year = "2021",
abstract = "Freeze drying was compared with spray drying regarding feasibility to process wild thyme drugs in order to obtain dry formulations at laboratory scale starting from liquid extracts produced by different extraction methods: maceration and heat-, ultrasound-, and microwave-assisted extractions. Higher total powder yield (based on the dry weight prior to extraction) was achieved by freeze than spray drying and lower loss of total polyphenol content (TPC) and total flavonoid content (TFC) due to the drying process. Gelatin as a coating agent (5% w/w) provided better TPC recovery by 70% in case of lyophilization and higher total powder yield in case of spray drying by diminishing material deposition on the wall of the drying chamber. The resulting gelatin-free and gelatin-containing powders carried polyphenols in amount similar to 190 and 53-75 mg gallic acid equivalents GAE/g of powder, respectively. Microwave-assisted extract formulation was distinguished from the others by a higher content of polyphenols, proteins and sugars, higher bulk density and lower solubility. The type of the drying process mainly affected the position of the gelatin-derived -OH and amide bands in FTIR spectra. Spray-dried formulations compared to freeze-dried expressed higher thermal stability as confirmed by differential scanning calorimetry analysis and a higher diffusion coefficient; the last feature can be associated with the lower specific surface area of irregularly shaped freeze-dried particles (151-223 mu m) compared to small microspheres (similar to 8 mu m) in spray-dried powder.",
journal = "Molecules",
title = "Freeze vs. Spray Drying for Dry Wild Thyme (Thymus serpyllum L.) Extract Formulations: The Impact of Gelatin as a Coating Material",
number = "13",
volume = "26",
doi = "10.3390/molecules26133933"
}

Jovanović, A., Levic, S. M., Pavlović, V. B., Markovic, S. B., Pjanović, R., Đorđević, V., Nedović, V.,& Bugarski, B.. (2021). Freeze vs. Spray Drying for Dry Wild Thyme (Thymus serpyllum L.) Extract Formulations: The Impact of Gelatin as a Coating Material. in Molecules, 26(13).
https://doi.org/10.3390/molecules26133933

Jovanović A, Levic SM, Pavlović VB, Markovic SB, Pjanović R, Đorđević V, Nedović V, Bugarski B. Freeze vs. Spray Drying for Dry Wild Thyme (Thymus serpyllum L.) Extract Formulations: The Impact of Gelatin as a Coating Material. in Molecules. 2021;26(13).
doi:10.3390/molecules26133933 .

Jovanović, Aleksandra, Levic, Steva M., Pavlović, Vladimir B., Markovic, Smilja B., Pjanović, Rada, Đorđević, Verica, Nedović, Viktor, Bugarski, Branko, "Freeze vs. Spray Drying for Dry Wild Thyme (Thymus serpyllum L.) Extract Formulations: The Impact of Gelatin as a Coating Material" in Molecules, 26, no. 13 (2021),
https://doi.org/10.3390/molecules26133933 . .

TY  - CONF
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Skenderija, Jovana
AU  - Vukoičić, Ana
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6049
AB  - According to the current definition, a prebiotic is described as “a substrate that is selectively utilized by host microorganisms conferring a health benefit”. Originally, the concept of prebiotics was defined for the gut microbiota. However, it can be also applied to modulate the composition of other microbiological populations, for example, skin microbiota. In recent years, scientific interest in the composition and function of the dermal microbiota has been growing, as it has been established that certain skin diseases are associated with dysbiosis of the skin microbiota. Consequently, for the cosmetics industry, it is of utmost importance to develop products that have a prebiotic effect and thus can provide a balanced composition of the skin microbiota. In this paper, an assessment of the galacto-oligosaccharides (GOS) prebiotic activity was performed by following the effect of the addition of these oligosaccharides on the growth of Staphylococcus epidermidis, a member of the permanent skin microbiota. Simultaneously, changes in nutrient medium composition during the fermentation were monitored using high-performance liquid chromatography. In the end, bearing in mind the future implementation of GOS in cosmetic formulations, the possibility of controlled release was determined using the Franz cell diffusion study. The obtained results showed that GOS has a stimulating effect on the growth of S. epidermidis since it has been noticed that the number of vegetative cells increases with increasing concentration of GOS up to the concentration of 5 % (w/v). Based on the change in the composition of soluble sugars in nutrient media enriched with oligosaccharides, it was confirmed that the bacteria S. epidermidis can utilize GOS. Finally, the effective diffusion coefficients of GOS, that have been incorporated into two different cosmetic formulations for previously defined optimal concentration (5 % w/v), were determined. Namely, the best results were obtained for formulation with Heliogel as a gelling agent - the effective diffusion coefficients of GOS trisaccharides (GOS3) and GOS tetrasaccharides (GOS4) were 1.904·10-6 cm2/s and 4.696·10-9 cm2/s, respectively. On the other hand, the effective diffusion coefficient of GOS3 in hydrogel Aristoflex AVC was 5.148·10-8 cm2/s, while the diffusion coefficient of GOS4 could not be determined, due to the low diffused concentrations. This indicates that GOS3 diffuse significantly faster than GOS4 and the rate and mechanism of transport of these molecules are highly dependent upon the type and characteristics of the formulation.
PB  - Faculty of Technology, University of East Sarajevo
C3  - VII International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, Republic of Srpska Bosnia and Hercegovina, Book of abstracts
T1  - EFFECT OF GALACTO-OLIGOSACCHARIDES ON STAPHYLOCOCCUS EPIDERMIDIS GROWTH AND EXAMINATION OF THEIR DIFFUSION FROM COSMETIC FORMULATIONS
IS  - ENG-97
SP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6049
ER  - 

@conference{
author = "Petrov Ivanković, Anja and Ćorović, Marija and Milivojević, Ana and Skenderija, Jovana and Vukoičić, Ana and Pjanović, Rada and Bezbradica, Dejan",
year = "2021",
abstract = "According to the current definition, a prebiotic is described as “a substrate that is selectively utilized by host microorganisms conferring a health benefit”. Originally, the concept of prebiotics was defined for the gut microbiota. However, it can be also applied to modulate the composition of other microbiological populations, for example, skin microbiota. In recent years, scientific interest in the composition and function of the dermal microbiota has been growing, as it has been established that certain skin diseases are associated with dysbiosis of the skin microbiota. Consequently, for the cosmetics industry, it is of utmost importance to develop products that have a prebiotic effect and thus can provide a balanced composition of the skin microbiota. In this paper, an assessment of the galacto-oligosaccharides (GOS) prebiotic activity was performed by following the effect of the addition of these oligosaccharides on the growth of Staphylococcus epidermidis, a member of the permanent skin microbiota. Simultaneously, changes in nutrient medium composition during the fermentation were monitored using high-performance liquid chromatography. In the end, bearing in mind the future implementation of GOS in cosmetic formulations, the possibility of controlled release was determined using the Franz cell diffusion study. The obtained results showed that GOS has a stimulating effect on the growth of S. epidermidis since it has been noticed that the number of vegetative cells increases with increasing concentration of GOS up to the concentration of 5 % (w/v). Based on the change in the composition of soluble sugars in nutrient media enriched with oligosaccharides, it was confirmed that the bacteria S. epidermidis can utilize GOS. Finally, the effective diffusion coefficients of GOS, that have been incorporated into two different cosmetic formulations for previously defined optimal concentration (5 % w/v), were determined. Namely, the best results were obtained for formulation with Heliogel as a gelling agent - the effective diffusion coefficients of GOS trisaccharides (GOS3) and GOS tetrasaccharides (GOS4) were 1.904·10-6 cm2/s and 4.696·10-9 cm2/s, respectively. On the other hand, the effective diffusion coefficient of GOS3 in hydrogel Aristoflex AVC was 5.148·10-8 cm2/s, while the diffusion coefficient of GOS4 could not be determined, due to the low diffused concentrations. This indicates that GOS3 diffuse significantly faster than GOS4 and the rate and mechanism of transport of these molecules are highly dependent upon the type and characteristics of the formulation.",
publisher = "Faculty of Technology, University of East Sarajevo",
journal = "VII International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, Republic of Srpska Bosnia and Hercegovina, Book of abstracts",
title = "EFFECT OF GALACTO-OLIGOSACCHARIDES ON STAPHYLOCOCCUS EPIDERMIDIS GROWTH AND EXAMINATION OF THEIR DIFFUSION FROM COSMETIC FORMULATIONS",
number = "ENG-97",
pages = "140",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6049"
}

Petrov Ivanković, A., Ćorović, M., Milivojević, A., Skenderija, J., Vukoičić, A., Pjanović, R.,& Bezbradica, D.. (2021). EFFECT OF GALACTO-OLIGOSACCHARIDES ON STAPHYLOCOCCUS EPIDERMIDIS GROWTH AND EXAMINATION OF THEIR DIFFUSION FROM COSMETIC FORMULATIONS. in VII International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, Republic of Srpska Bosnia and Hercegovina, Book of abstracts
Faculty of Technology, University of East Sarajevo.(ENG-97), 140.
https://hdl.handle.net/21.15107/rcub_technorep_6049

Petrov Ivanković A, Ćorović M, Milivojević A, Skenderija J, Vukoičić A, Pjanović R, Bezbradica D. EFFECT OF GALACTO-OLIGOSACCHARIDES ON STAPHYLOCOCCUS EPIDERMIDIS GROWTH AND EXAMINATION OF THEIR DIFFUSION FROM COSMETIC FORMULATIONS. in VII International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, Republic of Srpska Bosnia and Hercegovina, Book of abstracts. 2021;(ENG-97):140.
https://hdl.handle.net/21.15107/rcub_technorep_6049 .

Petrov Ivanković, Anja, Ćorović, Marija, Milivojević, Ana, Skenderija, Jovana, Vukoičić, Ana, Pjanović, Rada, Bezbradica, Dejan, "EFFECT OF GALACTO-OLIGOSACCHARIDES ON STAPHYLOCOCCUS EPIDERMIDIS GROWTH AND EXAMINATION OF THEIR DIFFUSION FROM COSMETIC FORMULATIONS" in VII International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, Republic of Srpska Bosnia and Hercegovina, Book of abstracts, no. ENG-97 (2021):140,
https://hdl.handle.net/21.15107/rcub_technorep_6049 .

TY  - CONF
AU  - Marković, Maja D.
AU  - Panić, Vesna V.
AU  - Seslija, Sanja I.
AU  - Pjanović, Rada V.
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5072
AB  - Polymeric carriers for targeted delivery of poorly water-soluble active substances (PWSAS)
are facing two challenges. First, encapsulation of PWSAS depends on structure of the carrier. Second
limitation is caused by the phenomenon of burst drug release. Despite the fact that the fast release
of drug in a burst stage is utilized in certain drug administration strategies, the negative effects
brought about by burst can be pharmacologically dangerous and economically inefficient. This study
presents a novel strategy for encapsulation and targeted delivery of PWSAS - caffeine without the
possibility of burst effect. Hydrophilic carrier based on poly(methacylic acid) was modified by amphiphilic substances - casein and centrifuged liposomes to ensure caffeine encapsulation. The caffeine was encapsulated in liposomes which further were incorporated into the carrier networks during the synthesis of the carriers. The release kinetic of caffeine was analyzed with respect to morphology of the carriers and interactions that could be established between the components of the
carrier. The swelling of carriers and release of caffeine were further investigated depending on the
neutralization degree of methacrylic acid in two media with different pH simulating the path of the
carrier through the upper and lower human gastrointestinal tract. Synthesized carriers showed significant potential for encapsulation and targeted delivery of PWSAS.
AB  - Kod razvoja polimernih nosača za ciljano otpuštanje slabo vodorastvornih aktivnih supstanci
(SVAS) postoje dva ograničenja. Prvo, inkapsulacija SVAS zavisi od strukture nosača. Drugo ograničenje je vezano za naglo oslobađanje leka. Uprkos činjenici da je naglo oslobađanje leka, tzv. burst
efekat poželjno kod nekih sistema, negativni efekti naglog oslobađanja leka u većini slučajeva mogu
biti farmakološki opasni i ekonomski neefikasni. U ovom radu je prikazana nova strategija za inkapsulaciju i ciljano otpuštanje SVAS – kofeina bez burst efekta. Hidrofilni nosač na bazi poli(metakrilne
kiseline) je modifikovan amfifilnim supstancama – kazeinom i centrifugiranim lipozomima kako bi se
postigla inkapsulacija kofeina. Kofein je inkapsuliran u lipozome koji su zatim inkorporirani u mrežu
nosača tokom sinteze nosača. Analiziran je uticaj morfologije nosača i interakcija koje su se uspostavile između njegovih komponenti na kinetiku otpuštanja kofeina. Zatim je analiziran uticaj stepen
neutralizacije metakrilne kiseline na bubrenje nosača i otpuštanje kofeina u dve sredine različitih pH
vrednosti koje su simulirale pH sredinu u gastrointestinalnom traktu čoveka. Pokazano je da sintetisani nosači imaju veliki potencijal za inkapsulaciju i ciljano otpuštanje SVAS
PB  - Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku.
C3  - 33 RD International Congress On Process Industry
T1  - pH-sensitive hydrogels based on poly(methacrylic acid), casein and liposomes for targeted delivery of poorly water-soluble active substances
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5072
ER  - 

@conference{
author = "Marković, Maja D. and Panić, Vesna V. and Seslija, Sanja I. and Pjanović, Rada V.",
year = "2020",
abstract = "Polymeric carriers for targeted delivery of poorly water-soluble active substances (PWSAS)
are facing two challenges. First, encapsulation of PWSAS depends on structure of the carrier. Second
limitation is caused by the phenomenon of burst drug release. Despite the fact that the fast release
of drug in a burst stage is utilized in certain drug administration strategies, the negative effects
brought about by burst can be pharmacologically dangerous and economically inefficient. This study
presents a novel strategy for encapsulation and targeted delivery of PWSAS - caffeine without the
possibility of burst effect. Hydrophilic carrier based on poly(methacylic acid) was modified by amphiphilic substances - casein and centrifuged liposomes to ensure caffeine encapsulation. The caffeine was encapsulated in liposomes which further were incorporated into the carrier networks during the synthesis of the carriers. The release kinetic of caffeine was analyzed with respect to morphology of the carriers and interactions that could be established between the components of the
carrier. The swelling of carriers and release of caffeine were further investigated depending on the
neutralization degree of methacrylic acid in two media with different pH simulating the path of the
carrier through the upper and lower human gastrointestinal tract. Synthesized carriers showed significant potential for encapsulation and targeted delivery of PWSAS., Kod razvoja polimernih nosača za ciljano otpuštanje slabo vodorastvornih aktivnih supstanci
(SVAS) postoje dva ograničenja. Prvo, inkapsulacija SVAS zavisi od strukture nosača. Drugo ograničenje je vezano za naglo oslobađanje leka. Uprkos činjenici da je naglo oslobađanje leka, tzv. burst
efekat poželjno kod nekih sistema, negativni efekti naglog oslobađanja leka u većini slučajeva mogu
biti farmakološki opasni i ekonomski neefikasni. U ovom radu je prikazana nova strategija za inkapsulaciju i ciljano otpuštanje SVAS – kofeina bez burst efekta. Hidrofilni nosač na bazi poli(metakrilne
kiseline) je modifikovan amfifilnim supstancama – kazeinom i centrifugiranim lipozomima kako bi se
postigla inkapsulacija kofeina. Kofein je inkapsuliran u lipozome koji su zatim inkorporirani u mrežu
nosača tokom sinteze nosača. Analiziran je uticaj morfologije nosača i interakcija koje su se uspostavile između njegovih komponenti na kinetiku otpuštanja kofeina. Zatim je analiziran uticaj stepen
neutralizacije metakrilne kiseline na bubrenje nosača i otpuštanje kofeina u dve sredine različitih pH
vrednosti koje su simulirale pH sredinu u gastrointestinalnom traktu čoveka. Pokazano je da sintetisani nosači imaju veliki potencijal za inkapsulaciju i ciljano otpuštanje SVAS",
publisher = "Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku.",
journal = "33 RD International Congress On Process Industry",
title = "pH-sensitive hydrogels based on poly(methacrylic acid), casein and liposomes for targeted delivery of poorly water-soluble active substances",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5072"
}

Marković, M. D., Panić, V. V., Seslija, S. I.,& Pjanović, R. V.. (2020). pH-sensitive hydrogels based on poly(methacrylic acid), casein and liposomes for targeted delivery of poorly water-soluble active substances. in 33 RD International Congress On Process Industry
Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku...
https://hdl.handle.net/21.15107/rcub_technorep_5072

Marković MD, Panić VV, Seslija SI, Pjanović RV. pH-sensitive hydrogels based on poly(methacrylic acid), casein and liposomes for targeted delivery of poorly water-soluble active substances. in 33 RD International Congress On Process Industry. 2020;.
https://hdl.handle.net/21.15107/rcub_technorep_5072 .

Marković, Maja D., Panić, Vesna V., Seslija, Sanja I., Pjanović, Rada V., "pH-sensitive hydrogels based on poly(methacrylic acid), casein and liposomes for targeted delivery of poorly water-soluble active substances" in 33 RD International Congress On Process Industry (2020),
https://hdl.handle.net/21.15107/rcub_technorep_5072 .

TY  - JOUR
AU  - Marković, Maja
AU  - Panić, Vesna
AU  - Šešlija, Sanja
AU  - Spasojević, Pavle
AU  - Ugrinović, Vukašin
AU  - Bošković-Vragolović, Nevenka
AU  - Pjanović, Rada
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4382
AB  - pH sensitive, nontoxic, and biocompatible poly(methacrylic) acid (PMAA) based soft networks have been extensively used in the design of systems for targeted drug delivery. Still, their highly hydrophilic nature limits their potential to be used as a carrier of poorly water-soluble substances. With the aim to overcome this limitation, the present study details a new approach for modification of PMAA based carriers using two amphiphilic components: casein and liposomes. The FTIR analysis revealed structural features of each component as well as the synergetic effect that originated from the formation of specific interactions. Namely, hydrophobic interactions between the poorly water-soluble model drug (caffeine) and casein enabled caffeine encapsulation and controlled release, while addition of liposomes ensured better control of the release rate. The morphological properties of the carriers, swelling behavior, and release kinetics of caffeine were investigated depending on the variable synthesis parameters (neutralization degree of methacrylic acid, concentration of caffeine, presence/absence of liposomes) in two different media simulating the pH environment of human intestines and stomach. The data obtained from in vitro caffeine release were correlated and analyzed in detail using several mathematical models, indicating significant potential of investigated carriers for targeted delivery and controlled release of poorly water-soluble substances.
PB  - Wiley, Hoboken
T2  - Polymer Engineering and Science
T1  - Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance
EP  - 2510
IS  - 10
SP  - 2496
VL  - 60
DO  - 10.1002/pen.25487
ER  - 

@article{
author = "Marković, Maja and Panić, Vesna and Šešlija, Sanja and Spasojević, Pavle and Ugrinović, Vukašin and Bošković-Vragolović, Nevenka and Pjanović, Rada",
year = "2020",
abstract = "pH sensitive, nontoxic, and biocompatible poly(methacrylic) acid (PMAA) based soft networks have been extensively used in the design of systems for targeted drug delivery. Still, their highly hydrophilic nature limits their potential to be used as a carrier of poorly water-soluble substances. With the aim to overcome this limitation, the present study details a new approach for modification of PMAA based carriers using two amphiphilic components: casein and liposomes. The FTIR analysis revealed structural features of each component as well as the synergetic effect that originated from the formation of specific interactions. Namely, hydrophobic interactions between the poorly water-soluble model drug (caffeine) and casein enabled caffeine encapsulation and controlled release, while addition of liposomes ensured better control of the release rate. The morphological properties of the carriers, swelling behavior, and release kinetics of caffeine were investigated depending on the variable synthesis parameters (neutralization degree of methacrylic acid, concentration of caffeine, presence/absence of liposomes) in two different media simulating the pH environment of human intestines and stomach. The data obtained from in vitro caffeine release were correlated and analyzed in detail using several mathematical models, indicating significant potential of investigated carriers for targeted delivery and controlled release of poorly water-soluble substances.",
publisher = "Wiley, Hoboken",
journal = "Polymer Engineering and Science",
title = "Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance",
pages = "2510-2496",
number = "10",
volume = "60",
doi = "10.1002/pen.25487"
}

Marković, M., Panić, V., Šešlija, S., Spasojević, P., Ugrinović, V., Bošković-Vragolović, N.,& Pjanović, R.. (2020). Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance. in Polymer Engineering and Science
Wiley, Hoboken., 60(10), 2496-2510.
https://doi.org/10.1002/pen.25487

Marković M, Panić V, Šešlija S, Spasojević P, Ugrinović V, Bošković-Vragolović N, Pjanović R. Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance. in Polymer Engineering and Science. 2020;60(10):2496-2510.
doi:10.1002/pen.25487 .

Marković, Maja, Panić, Vesna, Šešlija, Sanja, Spasojević, Pavle, Ugrinović, Vukašin, Bošković-Vragolović, Nevenka, Pjanović, Rada, "Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance" in Polymer Engineering and Science, 60, no. 10 (2020):2496-2510,
https://doi.org/10.1002/pen.25487 . .