TY  - JOUR
AU  - Obradović, Bojana
AU  - Radisić, Milica
AU  - Vunjak-Novaković, Gordana
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1542
AB  - Tissue engineering is an attractive strategy to address the increasing clinical need for tissue replacement. Engineered tissues can also serve as high-fidelity models for studies of development, disease and therapeutic modalities. Cultivation of three-dimensional tissue equivalents is necessarily based on the use of bioreactors, which are designed to provide controlled steady state cultivation conditions as well as required biochemical and physical regulatory signals. In this chapter, we review the design and operation of tissue engineering bioreactors, with the focus on biomimetic approaches to provide in vivo-like environments for rapid and orderly tissue development by cells cultured on a scaffold. Specifically, we focus on bioreactors for tissue engineering of two distinctly different tissues - articular cartilage and myocardium.
T2  - NATO Science for Peace and Security Series A: Chemistry and Biology
T1  - Biomimetic Approaches to Design of Tissue Engineering Bioreactors
EP  - 129
SP  - 115
DO  - 10.1007/978-90-481-8790-4-7
ER  - 

@article{
author = "Obradović, Bojana and Radisić, Milica and Vunjak-Novaković, Gordana",
year = "2010",
abstract = "Tissue engineering is an attractive strategy to address the increasing clinical need for tissue replacement. Engineered tissues can also serve as high-fidelity models for studies of development, disease and therapeutic modalities. Cultivation of three-dimensional tissue equivalents is necessarily based on the use of bioreactors, which are designed to provide controlled steady state cultivation conditions as well as required biochemical and physical regulatory signals. In this chapter, we review the design and operation of tissue engineering bioreactors, with the focus on biomimetic approaches to provide in vivo-like environments for rapid and orderly tissue development by cells cultured on a scaffold. Specifically, we focus on bioreactors for tissue engineering of two distinctly different tissues - articular cartilage and myocardium.",
journal = "NATO Science for Peace and Security Series A: Chemistry and Biology",
title = "Biomimetic Approaches to Design of Tissue Engineering Bioreactors",
pages = "129-115",
doi = "10.1007/978-90-481-8790-4-7"
}

Obradović, B., Radisić, M.,& Vunjak-Novaković, G.. (2010). Biomimetic Approaches to Design of Tissue Engineering Bioreactors. in NATO Science for Peace and Security Series A: Chemistry and Biology, 115-129.
https://doi.org/10.1007/978-90-481-8790-4-7

Obradović B, Radisić M, Vunjak-Novaković G. Biomimetic Approaches to Design of Tissue Engineering Bioreactors. in NATO Science for Peace and Security Series A: Chemistry and Biology. 2010;:115-129.
doi:10.1007/978-90-481-8790-4-7 .

Obradović, Bojana, Radisić, Milica, Vunjak-Novaković, Gordana, "Biomimetic Approaches to Design of Tissue Engineering Bioreactors" in NATO Science for Peace and Security Series A: Chemistry and Biology (2010):115-129,
https://doi.org/10.1007/978-90-481-8790-4-7 . .

TY  - JOUR
AU  - Petrović, Miloš
AU  - Mitraković, Dragan
AU  - Bugarski, Branko
AU  - Vonwil, Daniel
AU  - Martin, Ivan
AU  - Obradović, Bojana
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1453
AB  - The provision of mechanical stimulation is believed to be necessary for the functional assembly of skeletal tissues, which are normally exposed to a variety of biomechanical signals in vivo. In this paper, we present a development and validation of a novel bioreactor aimed for skeletal tissue engineering that provides dynamic compression and perfusion of cultivated tissues. Dynamic compression can be applied at frequencies up to 67.5 Hz and displacements down to 5 µm thus suitable for the simulation of physiological conditions in a native cartilage tissue (0.1-1 Hz, 5-10 % strain). The bioreactor also includes a load sensor that was calibrated so to measure average loads imposed on tissue samples. Regimes of the mechanical stimulation and acquisition of load sensor outputs are directed by an automatic control system using applications developed within the LabView platform. In addition, perfusion of tissue samples at physiological velocities (10-100 µm/s) provides efficient mass transfer, as well as the possibilities to expose the cells to hydrodynamic shear and simulate the conditions in a native bone tissue. Thus, the novel bioreactor is suited for studies of the effects of different biomechanical signals on in vitro regeneration of skeletal tissues, as well as for the studies of newly formulated biomaterials and cell biomaterial interactions under in vivo-like settings.
PB  - Association of the Chemical Engineers of Serbia
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - A novel bioreactor with mechanical stimulation for skeletal tissue engineering
EP  - 44
IS  - 1
SP  - 41
VL  - 15
DO  - 10.2298/CICEQ0901041P
ER  - 

@article{
author = "Petrović, Miloš and Mitraković, Dragan and Bugarski, Branko and Vonwil, Daniel and Martin, Ivan and Obradović, Bojana",
year = "2009",
abstract = "The provision of mechanical stimulation is believed to be necessary for the functional assembly of skeletal tissues, which are normally exposed to a variety of biomechanical signals in vivo. In this paper, we present a development and validation of a novel bioreactor aimed for skeletal tissue engineering that provides dynamic compression and perfusion of cultivated tissues. Dynamic compression can be applied at frequencies up to 67.5 Hz and displacements down to 5 µm thus suitable for the simulation of physiological conditions in a native cartilage tissue (0.1-1 Hz, 5-10 % strain). The bioreactor also includes a load sensor that was calibrated so to measure average loads imposed on tissue samples. Regimes of the mechanical stimulation and acquisition of load sensor outputs are directed by an automatic control system using applications developed within the LabView platform. In addition, perfusion of tissue samples at physiological velocities (10-100 µm/s) provides efficient mass transfer, as well as the possibilities to expose the cells to hydrodynamic shear and simulate the conditions in a native bone tissue. Thus, the novel bioreactor is suited for studies of the effects of different biomechanical signals on in vitro regeneration of skeletal tissues, as well as for the studies of newly formulated biomaterials and cell biomaterial interactions under in vivo-like settings.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "A novel bioreactor with mechanical stimulation for skeletal tissue engineering",
pages = "44-41",
number = "1",
volume = "15",
doi = "10.2298/CICEQ0901041P"
}

Petrović, M., Mitraković, D., Bugarski, B., Vonwil, D., Martin, I.,& Obradović, B.. (2009). A novel bioreactor with mechanical stimulation for skeletal tissue engineering. in Chemical Industry & Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 15(1), 41-44.
https://doi.org/10.2298/CICEQ0901041P

Petrović M, Mitraković D, Bugarski B, Vonwil D, Martin I, Obradović B. A novel bioreactor with mechanical stimulation for skeletal tissue engineering. in Chemical Industry & Chemical Engineering Quarterly. 2009;15(1):41-44.
doi:10.2298/CICEQ0901041P .

Petrović, Miloš, Mitraković, Dragan, Bugarski, Branko, Vonwil, Daniel, Martin, Ivan, Obradović, Bojana, "A novel bioreactor with mechanical stimulation for skeletal tissue engineering" in Chemical Industry & Chemical Engineering Quarterly, 15, no. 1 (2009):41-44,
https://doi.org/10.2298/CICEQ0901041P . .