TY  - JOUR
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gökhan
AU  - Cvijetić, Ilija
AU  - Gašić, Uroš
AU  - Čutović, Natalija
AU  - Pešić, Mirjana B.
AU  - Milinčić, Danijel D.
AU  - Carević, Tamara
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Marković, Tatjana
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7457
AB  - Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
PB  - MDPI
T2  - Pharmaceuticals
T1  - Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia
IS  - 4
SP  - 518
VL  - 17
DO  - 10.3390/ph17040518
ER  - 

@article{
author = "Batinić, Petar and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gökhan and Cvijetić, Ilija and Gašić, Uroš and Čutović, Natalija and Pešić, Mirjana B. and Milinčić, Danijel D. and Carević, Tamara and Marinković, Aleksandar and Bugarski, Branko and Marković, Tatjana",
year = "2024",
abstract = "Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.",
publisher = "MDPI",
journal = "Pharmaceuticals",
title = "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia",
number = "4",
pages = "518",
volume = "17",
doi = "10.3390/ph17040518"
}

Batinić, P., Jovanović, A., Stojković, D., Zengin, G., Cvijetić, I., Gašić, U., Čutović, N., Pešić, M. B., Milinčić, D. D., Carević, T., Marinković, A., Bugarski, B.,& Marković, T.. (2024). Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals
MDPI., 17(4), 518.
https://doi.org/10.3390/ph17040518

Batinić P, Jovanović A, Stojković D, Zengin G, Cvijetić I, Gašić U, Čutović N, Pešić MB, Milinčić DD, Carević T, Marinković A, Bugarski B, Marković T. Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals. 2024;17(4):518.
doi:10.3390/ph17040518 .

Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gökhan, Cvijetić, Ilija, Gašić, Uroš, Čutović, Natalija, Pešić, Mirjana B., Milinčić, Danijel D., Carević, Tamara, Marinković, Aleksandar, Bugarski, Branko, Marković, Tatjana, "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia" in Pharmaceuticals, 17, no. 4 (2024):518,
https://doi.org/10.3390/ph17040518 . .

TY  - JOUR
AU  - Rajab Elferjane, Muna
AU  - Jovanović, Aleksandra A.
AU  - Milutinović, Violeta
AU  - Čutović, Natalija
AU  - Jovanović Krivokuća, Milica
AU  - Marinković, Aleksandar
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6591
AB  - In the study, the optimization of the extraction from Aloe vera leaf waste was performed via varying solid-to-solvent ratio, solvent type, extraction time, and technique (maceration, heat-, ultrasound-, and microwave-assisted extractions—HAE, UAE, and MAE, respectively). The optimal extraction conditions for achieving the highest polyphenol content are a 1:30 ratio, 70% ethanol, and 30 min of HAE. Total flavonoid and protein contents were significantly higher in the extract from MAE, while total condensed tannin content was the highest in HAE. LC-MS analysis quantified 13 anthraquinone and chromone compounds. The variations in the FT-IR spectra of the extracts obtained by different extraction procedures are minor. The influence of extraction conditions on the antioxidant ability of the extracts depended on applied antioxidant assays. The extracts possessed medium inhibition properties against Staphylococcus aureus and weak inhibitory activity against Enterococcus feacalis. The extracts had stimulative effect on HaCaT cell viability. Regarding the extraction yield, there was a significant difference between the used extraction techniques (MAE > HAE > maceration and UAE). The presented study is an initial step in the production of polyphenol-rich extracts from A. vera leaf waste aimed to be used for the potential preparation of pharmaceutical and cosmetic formulations for the skin.
PB  - MDPI
T2  - Plants
T1  - From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities
IS  - 14
SP  - 2744
VL  - 12
DO  - 10.3390/plants12142744
ER  - 

@article{
author = "Rajab Elferjane, Muna and Jovanović, Aleksandra A. and Milutinović, Violeta and Čutović, Natalija and Jovanović Krivokuća, Milica and Marinković, Aleksandar",
year = "2023",
abstract = "In the study, the optimization of the extraction from Aloe vera leaf waste was performed via varying solid-to-solvent ratio, solvent type, extraction time, and technique (maceration, heat-, ultrasound-, and microwave-assisted extractions—HAE, UAE, and MAE, respectively). The optimal extraction conditions for achieving the highest polyphenol content are a 1:30 ratio, 70% ethanol, and 30 min of HAE. Total flavonoid and protein contents were significantly higher in the extract from MAE, while total condensed tannin content was the highest in HAE. LC-MS analysis quantified 13 anthraquinone and chromone compounds. The variations in the FT-IR spectra of the extracts obtained by different extraction procedures are minor. The influence of extraction conditions on the antioxidant ability of the extracts depended on applied antioxidant assays. The extracts possessed medium inhibition properties against Staphylococcus aureus and weak inhibitory activity against Enterococcus feacalis. The extracts had stimulative effect on HaCaT cell viability. Regarding the extraction yield, there was a significant difference between the used extraction techniques (MAE > HAE > maceration and UAE). The presented study is an initial step in the production of polyphenol-rich extracts from A. vera leaf waste aimed to be used for the potential preparation of pharmaceutical and cosmetic formulations for the skin.",
publisher = "MDPI",
journal = "Plants",
title = "From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities",
number = "14",
pages = "2744",
volume = "12",
doi = "10.3390/plants12142744"
}

Rajab Elferjane, M., Jovanović, A. A., Milutinović, V., Čutović, N., Jovanović Krivokuća, M.,& Marinković, A.. (2023). From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities. in Plants
MDPI., 12(14), 2744.
https://doi.org/10.3390/plants12142744

Rajab Elferjane M, Jovanović AA, Milutinović V, Čutović N, Jovanović Krivokuća M, Marinković A. From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities. in Plants. 2023;12(14):2744.
doi:10.3390/plants12142744 .

Rajab Elferjane, Muna, Jovanović, Aleksandra A., Milutinović, Violeta, Čutović, Natalija, Jovanović Krivokuća, Milica, Marinković, Aleksandar, "From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities" in Plants, 12, no. 14 (2023):2744,
https://doi.org/10.3390/plants12142744 . .

TY  - JOUR
AU  - Čutović, Natalija
AU  - Batinić, Petar
AU  - Marković, Tatjana
AU  - Radanović, Dragoja
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Jovanović, Aleksandra A.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7041
AB  - The presented study aimed to optimize polyphenol extraction from Satureja montana L. obtained from the experimental field of the Institute for Medicinal Plants Research ”Dr Josif Pančić”, Serbia, by varying the most important parameters for maceration, solid-to-solvent ratio, and extraction time. The obtained extracts were characterized regarding the total polyphenol content (TPC), total flavonoid content (TFC), antioxidant potential, extraction yield, conductivity, density, surface tension, and viscosity. The TPC and TFC were ~7 to 92 mg GAE (gallic acid equivalent)/g and 3.7 to 10.9 mg CE (catechin equivalent)/g, respectively. The highest extraction yield (86 %) as well as the highest antioxidant activities were obtained for the extracts prepared using a solid-to-solvent ratio of 1 g:50 cm3. On the other hand, the extraction time did not have a significant influence. The highest conductivity was measured in the extract prepared at a 1 g:10 cm3 ratio, while the highest density and surface tension were in the extract prepared at a 1 g:40 cm3 ratio (941 kg m-3 and 29.0 mN m-1, respectively). The highest viscosity was measured in the extract prepared at a 1 g: 20 cm3 ratio (2.89 mPa∙s). Our study shows the possibilities for the production of polyphenol-rich extract of S. montana which might be used in pharmaceutical, food, or cosmetic products.
AB  - Prikazana  studija  je  imala  za  cilj  da  optimizuje  ekstrakciju  polifenola  izbiljkeSatureja  montanaL.  sa oglednog  polja  Instituta  za proučavanjelekovitog bilja „Dr Josif Pančić“, Srbija, variranjem najvažnijih parametara  maceracije,  odnosa biljnog materijala i rastvarača,i  ekstrakcionogvremena.  Dobijeni ekstrakti su okarakterisani u pogleduukupnog sadržaja polifenola (engl.total total polyphenol content, TPC), ukupnog sadržaja flavonoida (engl.total  flavonoid  content,TFC),  antioksidativnog  potencijala, prinosa  ekstrakcije,  provodljivosti,  gustine,  površinskog  napona  i  viskoznosti. Vrednostiukupnog sadržaja polifenola(engl.Total  Polyphenol  Content -TPC)iukupnog sadržaja flavonoida (engl.Total Flavonoid  Content -TFC)su  bile  u  opsezima7,2-92,2  mg ekvivalenta  galne  kiseline  (engl.gallic  acid equivalentGAE) /g i 3,7-10,9 mgekvivalenta katehina(engl.catechinequivalentCE)/g, redom. Najveće antioksidativne  aktivnosti dobijenesu  u  ekstraktima  pripremljenim  u  odnosu biljnog  materijala  i rastvarača1g :50cm-3. Najveći prinos ekstrakcije takođe je dobijenu tom ekstraktu (86,0%), dok vreme ekstrakcije nije imalo značajan uticaj. Najveća  provodljivost  izmerena  je  u  ekstraktu  pripremljenom  u odnosu biljnog materijala i rastvarača1g :10cm-3. Najvećagustina i površinski napon su bili u ekstraktu pripremljenom u odnosu 1g :40cm-3(941 kg m-3i 29,0 mNm-1, redom). Najvećaviskoznostje izmerenau  ekstraktu  pripremljenom  u  odnosu  1g :20cm-3(2,89 mPa∙s). Naša studija pokazuje mogućnosti  za proizvodnju  polifenolima  bogatog  ekstraktabiljkeS.  montanakoji se može koristiti u farmaceutskim, prehrambenim ili kozmetičkim proizvodima.
PB  - Savez hemijskih inženjera Srbije
T2  - Hemijska industrija
T1  - Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts
T1  - Optimizacija procesa ekstrakcije iz biljke Satureja montanal.: fizičkohemijska karakterizacija ekstrakata
EP  - 263
IS  - 4
SP  - 251
VL  - 77
DO  - 10.2298/HEMIND221213020C
ER  - 

@article{
author = "Čutović, Natalija and Batinić, Petar and Marković, Tatjana and Radanović, Dragoja and Marinković, Aleksandar and Bugarski, Branko and Jovanović, Aleksandra A.",
year = "2023",
abstract = "The presented study aimed to optimize polyphenol extraction from Satureja montana L. obtained from the experimental field of the Institute for Medicinal Plants Research ”Dr Josif Pančić”, Serbia, by varying the most important parameters for maceration, solid-to-solvent ratio, and extraction time. The obtained extracts were characterized regarding the total polyphenol content (TPC), total flavonoid content (TFC), antioxidant potential, extraction yield, conductivity, density, surface tension, and viscosity. The TPC and TFC were ~7 to 92 mg GAE (gallic acid equivalent)/g and 3.7 to 10.9 mg CE (catechin equivalent)/g, respectively. The highest extraction yield (86 %) as well as the highest antioxidant activities were obtained for the extracts prepared using a solid-to-solvent ratio of 1 g:50 cm3. On the other hand, the extraction time did not have a significant influence. The highest conductivity was measured in the extract prepared at a 1 g:10 cm3 ratio, while the highest density and surface tension were in the extract prepared at a 1 g:40 cm3 ratio (941 kg m-3 and 29.0 mN m-1, respectively). The highest viscosity was measured in the extract prepared at a 1 g: 20 cm3 ratio (2.89 mPa∙s). Our study shows the possibilities for the production of polyphenol-rich extract of S. montana which might be used in pharmaceutical, food, or cosmetic products., Prikazana  studija  je  imala  za  cilj  da  optimizuje  ekstrakciju  polifenola  izbiljkeSatureja  montanaL.  sa oglednog  polja  Instituta  za proučavanjelekovitog bilja „Dr Josif Pančić“, Srbija, variranjem najvažnijih parametara  maceracije,  odnosa biljnog materijala i rastvarača,i  ekstrakcionogvremena.  Dobijeni ekstrakti su okarakterisani u pogleduukupnog sadržaja polifenola (engl.total total polyphenol content, TPC), ukupnog sadržaja flavonoida (engl.total  flavonoid  content,TFC),  antioksidativnog  potencijala, prinosa  ekstrakcije,  provodljivosti,  gustine,  površinskog  napona  i  viskoznosti. Vrednostiukupnog sadržaja polifenola(engl.Total  Polyphenol  Content -TPC)iukupnog sadržaja flavonoida (engl.Total Flavonoid  Content -TFC)su  bile  u  opsezima7,2-92,2  mg ekvivalenta  galne  kiseline  (engl.gallic  acid equivalentGAE) /g i 3,7-10,9 mgekvivalenta katehina(engl.catechinequivalentCE)/g, redom. Najveće antioksidativne  aktivnosti dobijenesu  u  ekstraktima  pripremljenim  u  odnosu biljnog  materijala  i rastvarača1g :50cm-3. Najveći prinos ekstrakcije takođe je dobijenu tom ekstraktu (86,0%), dok vreme ekstrakcije nije imalo značajan uticaj. Najveća  provodljivost  izmerena  je  u  ekstraktu  pripremljenom  u odnosu biljnog materijala i rastvarača1g :10cm-3. Najvećagustina i površinski napon su bili u ekstraktu pripremljenom u odnosu 1g :40cm-3(941 kg m-3i 29,0 mNm-1, redom). Najvećaviskoznostje izmerenau  ekstraktu  pripremljenom  u  odnosu  1g :20cm-3(2,89 mPa∙s). Naša studija pokazuje mogućnosti  za proizvodnju  polifenolima  bogatog  ekstraktabiljkeS.  montanakoji se može koristiti u farmaceutskim, prehrambenim ili kozmetičkim proizvodima.",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija",
title = "Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts, Optimizacija procesa ekstrakcije iz biljke Satureja montanal.: fizičkohemijska karakterizacija ekstrakata",
pages = "263-251",
number = "4",
volume = "77",
doi = "10.2298/HEMIND221213020C"
}

Čutović, N., Batinić, P., Marković, T., Radanović, D., Marinković, A., Bugarski, B.,& Jovanović, A. A.. (2023). Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts. in Hemijska industrija
Savez hemijskih inženjera Srbije., 77(4), 251-263.
https://doi.org/10.2298/HEMIND221213020C

Čutović N, Batinić P, Marković T, Radanović D, Marinković A, Bugarski B, Jovanović AA. Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts. in Hemijska industrija. 2023;77(4):251-263.
doi:10.2298/HEMIND221213020C .

Čutović, Natalija, Batinić, Petar, Marković, Tatjana, Radanović, Dragoja, Marinković, Aleksandar, Bugarski, Branko, Jovanović, Aleksandra A., "Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts" in Hemijska industrija, 77, no. 4 (2023):251-263,
https://doi.org/10.2298/HEMIND221213020C . .

TY  - CONF
AU  - Jovanović, Aleksandra
AU  - Volić, Mina
AU  - Obradović, Nataša
AU  - Bugarski, Branko
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6452
AB  - Polyphenol recovery and physico-chemical properties of the extracts depend on the type of
solvents and their pH values. Additionally, due to the presence of different biologically active
compounds in plant waste, plant extracts obtained from industrial by-processing can find
potential applications in various products. In the present study, Serpylli herba waste extracts
were prepared using maceration (60 min), a solid-to-solvent ratio of 1:30, the particle size of
plant waste 0.3 mm, and two types of the extraction medium: 50% ethanol (pH 6) and 50%
ethanol with glacial acetic acid (pH 2.5). The lyophilization process was chosen as the next
step (-75°C, 0.011 mbar, for 24 h). Comparison of liquid and lyophilized extracts prepared at
different pH values was done via analyzing total polyphenol content (TPC, Folin-Ciocalteu
method), total flavonoid content (TFC, colorimetric assay), antioxidant capacity (ABTS and
DPPH assays), zeta potential, and conductivity (photon correlation spectroscopy). TPC of
liquid extracts prepared at pH 2.5 and pH 6 amounted to 1.38 and 1.23 mg GAE/mL,
respectively, while lyophilized parallels had 271.7 and 188.8 mg GAE/g, respectively. The
same trend is noticed in the case of TFC: 0.368 and 0.334 mg CE/mL for liquid extracts
obtained at pH 2.5 and pH 6, respectively, and 102.5 and 99.7 mg CE/mL for lyophilized
parallels. ABTS radical scavenging activity of the liquid extracts at pH 2.5 and pH 6 was
0.767 and 0.750 mmol TE/L, respectively and for the lyophilized parallels was 0.136 and
0.111 mmol TE/g. IC50 (concentration for neutralization of 50% of DPPH free radicals) was
1.12 and 1.75 mg/mL for the liquid extracts prepared at pH 2.5 and pH 6, respectively and
0.331 and 0.391 mg/mL for the lyophilized parallels. The zeta potential of the liquid extracts
at pH 2.5 and pH 6 was 1.74 and -2.56 mV, respectively, whereas the zeta potential of
lyophilized parallels was -3.55 and -18.7 mV. Conductivity of the liquid extracts was 0.864(pH 2.5) and 0.423 mS/cm (pH 6), whereas for the lyophilized extracts it was 0.199 (pH 2.5)
and 0.452 mS/cm (pH 6). The presented results provide the information on physico-chemical
properties of Serpylli herba waste liquid and lyophilized extracts that can add value and
improve the quality of the existing food, functional food, pharmaceutical and cosmetic
products, as well as for drinking water and wastewater treatment.
PB  - Novi Sad : Faculty of Technology
C3  - Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad
T1  - COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES
EP  - 175
SP  - 166
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6452
ER  - 

@conference{
author = "Jovanović, Aleksandra and Volić, Mina and Obradović, Nataša and Bugarski, Branko",
year = "2023",
abstract = "Polyphenol recovery and physico-chemical properties of the extracts depend on the type of
solvents and their pH values. Additionally, due to the presence of different biologically active
compounds in plant waste, plant extracts obtained from industrial by-processing can find
potential applications in various products. In the present study, Serpylli herba waste extracts
were prepared using maceration (60 min), a solid-to-solvent ratio of 1:30, the particle size of
plant waste 0.3 mm, and two types of the extraction medium: 50% ethanol (pH 6) and 50%
ethanol with glacial acetic acid (pH 2.5). The lyophilization process was chosen as the next
step (-75°C, 0.011 mbar, for 24 h). Comparison of liquid and lyophilized extracts prepared at
different pH values was done via analyzing total polyphenol content (TPC, Folin-Ciocalteu
method), total flavonoid content (TFC, colorimetric assay), antioxidant capacity (ABTS and
DPPH assays), zeta potential, and conductivity (photon correlation spectroscopy). TPC of
liquid extracts prepared at pH 2.5 and pH 6 amounted to 1.38 and 1.23 mg GAE/mL,
respectively, while lyophilized parallels had 271.7 and 188.8 mg GAE/g, respectively. The
same trend is noticed in the case of TFC: 0.368 and 0.334 mg CE/mL for liquid extracts
obtained at pH 2.5 and pH 6, respectively, and 102.5 and 99.7 mg CE/mL for lyophilized
parallels. ABTS radical scavenging activity of the liquid extracts at pH 2.5 and pH 6 was
0.767 and 0.750 mmol TE/L, respectively and for the lyophilized parallels was 0.136 and
0.111 mmol TE/g. IC50 (concentration for neutralization of 50% of DPPH free radicals) was
1.12 and 1.75 mg/mL for the liquid extracts prepared at pH 2.5 and pH 6, respectively and
0.331 and 0.391 mg/mL for the lyophilized parallels. The zeta potential of the liquid extracts
at pH 2.5 and pH 6 was 1.74 and -2.56 mV, respectively, whereas the zeta potential of
lyophilized parallels was -3.55 and -18.7 mV. Conductivity of the liquid extracts was 0.864(pH 2.5) and 0.423 mS/cm (pH 6), whereas for the lyophilized extracts it was 0.199 (pH 2.5)
and 0.452 mS/cm (pH 6). The presented results provide the information on physico-chemical
properties of Serpylli herba waste liquid and lyophilized extracts that can add value and
improve the quality of the existing food, functional food, pharmaceutical and cosmetic
products, as well as for drinking water and wastewater treatment.",
publisher = "Novi Sad : Faculty of Technology",
journal = "Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad",
title = "COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES",
pages = "175-166",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6452"
}

Jovanović, A., Volić, M., Obradović, N.,& Bugarski, B.. (2023). COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES. in Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad
Novi Sad : Faculty of Technology., 166-175.
https://hdl.handle.net/21.15107/rcub_technorep_6452

Jovanović A, Volić M, Obradović N, Bugarski B. COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES. in Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad. 2023;:166-175.
https://hdl.handle.net/21.15107/rcub_technorep_6452 .

Jovanović, Aleksandra, Volić, Mina, Obradović, Nataša, Bugarski, Branko, "COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES" in Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad (2023):166-175,
https://hdl.handle.net/21.15107/rcub_technorep_6452 .

TY  - JOUR
AU  - Čutović, Natalija
AU  - Marković, Tatjana
AU  - Carević, Tamara
AU  - Stojković, Dejan
AU  - Bugarski, Branko
AU  - Jovanović, Aleksandra A.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7073
AB  - Paeonia tenuifolia L. (steppe peony) petal extract was proficiently encapsulated into liposomes and biopolymer films in the current work, both times utilizing a single-step procedure. The encapsulation efficiency, size of the particles, and index of polydispersity (PDI), as well as the ζ potential of the obtained liposomes were determined, whereas in the case of films, the test included moisture content and mechanical property assessment. Fourier transform infrared spectroscopy (FT-IR) was used to evaluate the chemical composition and existence of numerous interactions in the systems. All the obtained encapsulates were subjected to antibacterial, antifungal and antibiofilm activity testing of the pathogens associated with human skin. The results indicated that the liposomes prepared using Phospholipon had the highest encapsulation efficiency (72.04%), making them the most favorable ones in the release study as well. The biological assays also revealed that Phospholipon was the most beneficial phospholipid mixture for the preparation of liposomes, whereas the film containing these liposomes did not have the ability to inhibit pathogen growth, making the double encapsulation of P. tenuifolia L. petal extract needless. These findings may be a first step toward the potential use of steppe peony extract-loaded films and liposomes in pharmaceutical and cosmetical industries.
PB  - MDPI
T2  - Pharmaceutics
T1  - Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential
IS  - 12
SP  - 2742
VL  - 15
DO  - 10.3390/pharmaceutics15122742
ER  - 

@article{
author = "Čutović, Natalija and Marković, Tatjana and Carević, Tamara and Stojković, Dejan and Bugarski, Branko and Jovanović, Aleksandra A.",
year = "2023",
abstract = "Paeonia tenuifolia L. (steppe peony) petal extract was proficiently encapsulated into liposomes and biopolymer films in the current work, both times utilizing a single-step procedure. The encapsulation efficiency, size of the particles, and index of polydispersity (PDI), as well as the ζ potential of the obtained liposomes were determined, whereas in the case of films, the test included moisture content and mechanical property assessment. Fourier transform infrared spectroscopy (FT-IR) was used to evaluate the chemical composition and existence of numerous interactions in the systems. All the obtained encapsulates were subjected to antibacterial, antifungal and antibiofilm activity testing of the pathogens associated with human skin. The results indicated that the liposomes prepared using Phospholipon had the highest encapsulation efficiency (72.04%), making them the most favorable ones in the release study as well. The biological assays also revealed that Phospholipon was the most beneficial phospholipid mixture for the preparation of liposomes, whereas the film containing these liposomes did not have the ability to inhibit pathogen growth, making the double encapsulation of P. tenuifolia L. petal extract needless. These findings may be a first step toward the potential use of steppe peony extract-loaded films and liposomes in pharmaceutical and cosmetical industries.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential",
number = "12",
pages = "2742",
volume = "15",
doi = "10.3390/pharmaceutics15122742"
}

Čutović, N., Marković, T., Carević, T., Stojković, D., Bugarski, B.,& Jovanović, A. A.. (2023). Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential. in Pharmaceutics
MDPI., 15(12), 2742.
https://doi.org/10.3390/pharmaceutics15122742

Čutović N, Marković T, Carević T, Stojković D, Bugarski B, Jovanović AA. Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential. in Pharmaceutics. 2023;15(12):2742.
doi:10.3390/pharmaceutics15122742 .

Čutović, Natalija, Marković, Tatjana, Carević, Tamara, Stojković, Dejan, Bugarski, Branko, Jovanović, Aleksandra A., "Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential" in Pharmaceutics, 15, no. 12 (2023):2742,
https://doi.org/10.3390/pharmaceutics15122742 . .

TY  - JOUR
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana
AU  - Volić, Mina
AU  - Pećinar, Ilinka
AU  - Živković, Jelena
AU  - Šavikin, Katarina P.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6629
AB  - In the present study, rosehip (Rosa canina L.) extract was successfully encapsulated in phospholipid liposomes using a single-step procedure named the proliposome method. Part of the obtained liposomes was subjected to UV irradiation and non-treated (native) and UV-irradiated liposomes were further characterized in terms of encapsulation efficiency, chemical composition (HPLC analysis), antioxidant capacity, particle size, PDI, zeta potential, conductivity, mobility, and antioxidant capacity. Raman spectroscopy as well as DSC analysis were applied to evaluate the influence of UV irradiation on the physicochemical properties of liposomes. The encapsulation efficiency of extract-loaded liposomes was higher than 90%; the average size was 251.5 nm; the zeta potential was −22.4 mV; and the conductivity was found to be 0.007 mS/cm. UV irradiation did not cause a change in the mentioned parameters. In addition, irradiation did not affect the antioxidant potential of the liposome–extract system. Raman spectroscopy indicated that the extract was completely covered by the lipid membrane during liposome entrapment, and the peroxidation process was minimized by the presence of rosehip extract in liposomes. These results may guide the potential application of rosehip extract-loaded liposomes in the food, pharmaceutical, or cosmetic industries, particularly when liposomal sterilization is needed.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Plants
T1  - Rosehip Extract-Loaded Liposomes for Potential Skin Application: Physicochemical Properties of Non- and UV-Irradiated Liposomes
IS  - 17
SP  - 3063
VL  - 12
DO  - 10.3390/plants12173063
ER  - 

@article{
author = "Jovanović, Aleksandra A. and Balanč, Bojana and Volić, Mina and Pećinar, Ilinka and Živković, Jelena and Šavikin, Katarina P.",
year = "2023",
abstract = "In the present study, rosehip (Rosa canina L.) extract was successfully encapsulated in phospholipid liposomes using a single-step procedure named the proliposome method. Part of the obtained liposomes was subjected to UV irradiation and non-treated (native) and UV-irradiated liposomes were further characterized in terms of encapsulation efficiency, chemical composition (HPLC analysis), antioxidant capacity, particle size, PDI, zeta potential, conductivity, mobility, and antioxidant capacity. Raman spectroscopy as well as DSC analysis were applied to evaluate the influence of UV irradiation on the physicochemical properties of liposomes. The encapsulation efficiency of extract-loaded liposomes was higher than 90%; the average size was 251.5 nm; the zeta potential was −22.4 mV; and the conductivity was found to be 0.007 mS/cm. UV irradiation did not cause a change in the mentioned parameters. In addition, irradiation did not affect the antioxidant potential of the liposome–extract system. Raman spectroscopy indicated that the extract was completely covered by the lipid membrane during liposome entrapment, and the peroxidation process was minimized by the presence of rosehip extract in liposomes. These results may guide the potential application of rosehip extract-loaded liposomes in the food, pharmaceutical, or cosmetic industries, particularly when liposomal sterilization is needed.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Plants",
title = "Rosehip Extract-Loaded Liposomes for Potential Skin Application: Physicochemical Properties of Non- and UV-Irradiated Liposomes",
number = "17",
pages = "3063",
volume = "12",
doi = "10.3390/plants12173063"
}

Jovanović, A. A., Balanč, B., Volić, M., Pećinar, I., Živković, J.,& Šavikin, K. P.. (2023). Rosehip Extract-Loaded Liposomes for Potential Skin Application: Physicochemical Properties of Non- and UV-Irradiated Liposomes. in Plants
Multidisciplinary Digital Publishing Institute (MDPI)., 12(17), 3063.
https://doi.org/10.3390/plants12173063

Jovanović AA, Balanč B, Volić M, Pećinar I, Živković J, Šavikin KP. Rosehip Extract-Loaded Liposomes for Potential Skin Application: Physicochemical Properties of Non- and UV-Irradiated Liposomes. in Plants. 2023;12(17):3063.
doi:10.3390/plants12173063 .

Jovanović, Aleksandra A., Balanč, Bojana, Volić, Mina, Pećinar, Ilinka, Živković, Jelena, Šavikin, Katarina P., "Rosehip Extract-Loaded Liposomes for Potential Skin Application: Physicochemical Properties of Non- and UV-Irradiated Liposomes" in Plants, 12, no. 17 (2023):3063,
https://doi.org/10.3390/plants12173063 . .

TY  - CONF
AU  - Gruden-Movsesijan, Alisa
AU  - Tomić, Sergej
AU  - Ilić, Nataša
AU  - Đokić, Jelena
AU  - Glamočlija, Sofija
AU  - Vasilev, Saša
AU  - Sabljić, Ljiljana
AU  - Stojanović, Dušica
AU  - Miljković, Đorđe
AU  - Dinić, Miroslav
AU  - Radojević, Dušan
AU  - Jevtić, Bojan
AU  - Golić, Nataša
AU  - Uskoković, Petar
AU  - Sofronić-Milosavljević, Ljiljana
AU  - Čolić, Miodrag
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6671
AB  - Trichinella spiralis possess the potential to induce tolerance and restrain excessive immune responses, thus
exerting beneficial effect on the outcome of experimental autoimmune encephalomyelitis (EAE), the animal
model of human disease multiple sclerosis. Trichinella achieves this by continuous release of its excretoysecretory (ES L1) products in the form of a complex mixture of individual components and extracellular vesicles
(TsEVs), preferentially exsosomes. Our previous results suggested that the complex modulation of the host
immune response is achieved by ES L1 products or its individula components, which induce the maturation
of dendritic cells towards tolerogenic status. A new delivery system, based on nanomaterials as carriers of ES
L1, was designed to resemble what happens in nature, ie. spontaneous release of Trichinella products over
an extended period of time using a less invasive route of administration than all previously described for the
treatment of autoimmune diseases. PLGA biodegradable nanofibers loaded with ES L1 (PLGA-ES L1), were
used as subcutaneous implants. The tretment proved to be safe with no side effects, successful in delivering
parasite products and mitigating the course of relapsing/remitting EAE in Dark Agouti rats. The mechanisms
we examined included events at the systemic level and target tissue, spinal cord, that explain the shift from the
pro- to anti-inflammatory responses and success of treatment. PLGA-ES L1 treatment also prevents the EAEinduced disruption of intestinal epithelial barrier. It diminished the inflammation by lowering the expression
of TNF-α in gastrointestinal tract (GIT) and succesfully maintain the expression of mRNA for claudin, the most
important tight junction protein in GIT. Obseved protective effect was acompained with the re-establishment of gut microbiota diversity that was disturbed in EAE-induced animals.
PB  - Belgrade : Serbian Society for Parasitology
C3  - Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16
T1  - New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment
SP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6671
ER  - 

@conference{
author = "Gruden-Movsesijan, Alisa and Tomić, Sergej and Ilić, Nataša and Đokić, Jelena and Glamočlija, Sofija and Vasilev, Saša and Sabljić, Ljiljana and Stojanović, Dušica and Miljković, Đorđe and Dinić, Miroslav and Radojević, Dušan and Jevtić, Bojan and Golić, Nataša and Uskoković, Petar and Sofronić-Milosavljević, Ljiljana and Čolić, Miodrag",
year = "2021",
abstract = "Trichinella spiralis possess the potential to induce tolerance and restrain excessive immune responses, thus
exerting beneficial effect on the outcome of experimental autoimmune encephalomyelitis (EAE), the animal
model of human disease multiple sclerosis. Trichinella achieves this by continuous release of its excretoysecretory (ES L1) products in the form of a complex mixture of individual components and extracellular vesicles
(TsEVs), preferentially exsosomes. Our previous results suggested that the complex modulation of the host
immune response is achieved by ES L1 products or its individula components, which induce the maturation
of dendritic cells towards tolerogenic status. A new delivery system, based on nanomaterials as carriers of ES
L1, was designed to resemble what happens in nature, ie. spontaneous release of Trichinella products over
an extended period of time using a less invasive route of administration than all previously described for the
treatment of autoimmune diseases. PLGA biodegradable nanofibers loaded with ES L1 (PLGA-ES L1), were
used as subcutaneous implants. The tretment proved to be safe with no side effects, successful in delivering
parasite products and mitigating the course of relapsing/remitting EAE in Dark Agouti rats. The mechanisms
we examined included events at the systemic level and target tissue, spinal cord, that explain the shift from the
pro- to anti-inflammatory responses and success of treatment. PLGA-ES L1 treatment also prevents the EAEinduced disruption of intestinal epithelial barrier. It diminished the inflammation by lowering the expression
of TNF-α in gastrointestinal tract (GIT) and succesfully maintain the expression of mRNA for claudin, the most
important tight junction protein in GIT. Obseved protective effect was acompained with the re-establishment of gut microbiota diversity that was disturbed in EAE-induced animals.",
publisher = "Belgrade : Serbian Society for Parasitology",
journal = "Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16",
title = "New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment",
pages = "92",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6671"
}

Gruden-Movsesijan, A., Tomić, S., Ilić, N., Đokić, J., Glamočlija, S., Vasilev, S., Sabljić, L., Stojanović, D., Miljković, Đ., Dinić, M., Radojević, D., Jevtić, B., Golić, N., Uskoković, P., Sofronić-Milosavljević, L.,& Čolić, M.. (2021). New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment. in Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16
Belgrade : Serbian Society for Parasitology., 92.
https://hdl.handle.net/21.15107/rcub_technorep_6671

Gruden-Movsesijan A, Tomić S, Ilić N, Đokić J, Glamočlija S, Vasilev S, Sabljić L, Stojanović D, Miljković Đ, Dinić M, Radojević D, Jevtić B, Golić N, Uskoković P, Sofronić-Milosavljević L, Čolić M. New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment. in Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16. 2021;:92.
https://hdl.handle.net/21.15107/rcub_technorep_6671 .

Gruden-Movsesijan, Alisa, Tomić, Sergej, Ilić, Nataša, Đokić, Jelena, Glamočlija, Sofija, Vasilev, Saša, Sabljić, Ljiljana, Stojanović, Dušica, Miljković, Đorđe, Dinić, Miroslav, Radojević, Dušan, Jevtić, Bojan, Golić, Nataša, Uskoković, Petar, Sofronić-Milosavljević, Ljiljana, Čolić, Miodrag, "New delivery system for Trichinella spiralis antigens - accelerated approach to autoimmunity treatment" in Programme & Abstract Book / 13th European Multicolloquium of Parasitology Belgrade, October 12-16 (2021):92,
https://hdl.handle.net/21.15107/rcub_technorep_6671 .

TY  - JOUR
AU  - Lazić, Vesna
AU  - Pirković, Andrea
AU  - Sredojević, Dusan
AU  - Marković, Jelena
AU  - Papan, Jelena
AU  - Ahrenkiel, Phillip S.
AU  - Janković-Častvan, Ivona
AU  - Dekanski, Dragana
AU  - Jovanović-Krivokuća, Milica
AU  - Nedeljković, Jovan M.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4772
AB  - The toxicity of hybrid nanoparticles, consisting of non-toxic components, zirconium dioxide nanoparticles (ZrO2 NPs), and caffeic acid (CA), was examined against four different cell lines (HTR-8 SV/Neo, JEG-3, JAR, and HeLa). Stable aqueous ZrO2 sol, synthesized by forced hydrolysis, consists of 3-4 nm in size primary particles organized in 30-60 nm in size snowflake-like particles, as determined by transmission electron microscopy and direct light scattering measurements. The surface modification of ZrO2 NPs with CA leads to the formation of an interfacial charge transfer (ICT) complex followed by the appearance of absorption in the visible spectral range. The spectroscopic observations are complemented with the density functional theory calculations using a cluster model. The ZrO2 NPs and CA are non-toxic against four different cell lines in investigated concentration range. Also, ZrO2 NPs promote the proliferation of HTR-8 SV/Neo, JAR, and HeLa cells. On the other hand, hybrid ZrO2/CA NPs induced a significant reduction of the viability of the JEG-3 cells (39 %) for the high concentration of components (1.6 mM ZrO2 and 0.4 mM CA).
T2  - Chemico-Biological Interactions
T1  - Surface-modified ZrO2 nanoparticles with caffeic acid: Characterization and in vitro evaluation of biosafety for placental cells
VL  - 347
DO  - 10.1016/j.cbi.2021.109618
ER  - 

@article{
author = "Lazić, Vesna and Pirković, Andrea and Sredojević, Dusan and Marković, Jelena and Papan, Jelena and Ahrenkiel, Phillip S. and Janković-Častvan, Ivona and Dekanski, Dragana and Jovanović-Krivokuća, Milica and Nedeljković, Jovan M.",
year = "2021",
abstract = "The toxicity of hybrid nanoparticles, consisting of non-toxic components, zirconium dioxide nanoparticles (ZrO2 NPs), and caffeic acid (CA), was examined against four different cell lines (HTR-8 SV/Neo, JEG-3, JAR, and HeLa). Stable aqueous ZrO2 sol, synthesized by forced hydrolysis, consists of 3-4 nm in size primary particles organized in 30-60 nm in size snowflake-like particles, as determined by transmission electron microscopy and direct light scattering measurements. The surface modification of ZrO2 NPs with CA leads to the formation of an interfacial charge transfer (ICT) complex followed by the appearance of absorption in the visible spectral range. The spectroscopic observations are complemented with the density functional theory calculations using a cluster model. The ZrO2 NPs and CA are non-toxic against four different cell lines in investigated concentration range. Also, ZrO2 NPs promote the proliferation of HTR-8 SV/Neo, JAR, and HeLa cells. On the other hand, hybrid ZrO2/CA NPs induced a significant reduction of the viability of the JEG-3 cells (39 %) for the high concentration of components (1.6 mM ZrO2 and 0.4 mM CA).",
journal = "Chemico-Biological Interactions",
title = "Surface-modified ZrO2 nanoparticles with caffeic acid: Characterization and in vitro evaluation of biosafety for placental cells",
volume = "347",
doi = "10.1016/j.cbi.2021.109618"
}

Lazić, V., Pirković, A., Sredojević, D., Marković, J., Papan, J., Ahrenkiel, P. S., Janković-Častvan, I., Dekanski, D., Jovanović-Krivokuća, M.,& Nedeljković, J. M.. (2021). Surface-modified ZrO2 nanoparticles with caffeic acid: Characterization and in vitro evaluation of biosafety for placental cells. in Chemico-Biological Interactions, 347.
https://doi.org/10.1016/j.cbi.2021.109618

Lazić V, Pirković A, Sredojević D, Marković J, Papan J, Ahrenkiel PS, Janković-Častvan I, Dekanski D, Jovanović-Krivokuća M, Nedeljković JM. Surface-modified ZrO2 nanoparticles with caffeic acid: Characterization and in vitro evaluation of biosafety for placental cells. in Chemico-Biological Interactions. 2021;347.
doi:10.1016/j.cbi.2021.109618 .

Lazić, Vesna, Pirković, Andrea, Sredojević, Dusan, Marković, Jelena, Papan, Jelena, Ahrenkiel, Phillip S., Janković-Častvan, Ivona, Dekanski, Dragana, Jovanović-Krivokuća, Milica, Nedeljković, Jovan M., "Surface-modified ZrO2 nanoparticles with caffeic acid: Characterization and in vitro evaluation of biosafety for placental cells" in Chemico-Biological Interactions, 347 (2021),
https://doi.org/10.1016/j.cbi.2021.109618 . .