TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Buntić, Aneta
AU  - Milić, Marija
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/20
AB  - Purpose To avoid a negative environmental and economic impact of agricultural wastes, and following the principles of circular economy, the reuse of agricultural wastes is necessary. For this purpose, isolation of novel microorganisms with potential biotechnological application is recommended. The current researches in bioethanol production are aimed to reduce the production costs using low-cost substrates and in-house produced enzymes by novel isolated microorganisms. In line with this, in this study valorization of these agricultural by-products by novel isolate S. fulvissimus CKS7 to biotechnological value added products was done. Methods Standard microbiological methods were used for the isolation and characterization of strain. Enzymes activities were determinated using DNS method while, the ethanol concentration was determined based on the density of the alcohol distillate at 20 degrees C. Results The maximal enzymatic activities for amylase, cellulases (carboxymethyl cellulase and Avicelase), pectinase and xylanase were achieved using rye bran as a waste substrate for CKS7 growth. Obtained crude bacterial enzymes were used for enzymatic hydrolysis of lignocellulosic materials including horsetail waste, yellow gentian waste, corn stover, cotton material and corona pre-treated cotton material. The maximum yield of reducing sugars was obtained on horsetail waste and corona pre-treated cotton material. Waste brewer's yeast Saccharomyces cerevisiae was successfully used for the production of bioethanol using horsetail waste hydrolysate and corona pre-treated cotton material hydrolysate. Conclusion The obtained results showed that bacterial strain CKS7 has a significant, still unexplored enzymatic potential that could be used to achieve a cleaner, environmental friendly and economically acceptable biofuel production. [GRAPHICS] .
PB  - Springer, Dordrecht
T2  - Waste and Biomass Valorization
T1  - From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production
EP  - 174
IS  - 1
SP  - 165
VL  - 12
DO  - 10.1007/s12649-020-00960-3
ER  - 

@article{
author = "Mihajlovski, Katarina and Buntić, Aneta and Milić, Marija and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "Purpose To avoid a negative environmental and economic impact of agricultural wastes, and following the principles of circular economy, the reuse of agricultural wastes is necessary. For this purpose, isolation of novel microorganisms with potential biotechnological application is recommended. The current researches in bioethanol production are aimed to reduce the production costs using low-cost substrates and in-house produced enzymes by novel isolated microorganisms. In line with this, in this study valorization of these agricultural by-products by novel isolate S. fulvissimus CKS7 to biotechnological value added products was done. Methods Standard microbiological methods were used for the isolation and characterization of strain. Enzymes activities were determinated using DNS method while, the ethanol concentration was determined based on the density of the alcohol distillate at 20 degrees C. Results The maximal enzymatic activities for amylase, cellulases (carboxymethyl cellulase and Avicelase), pectinase and xylanase were achieved using rye bran as a waste substrate for CKS7 growth. Obtained crude bacterial enzymes were used for enzymatic hydrolysis of lignocellulosic materials including horsetail waste, yellow gentian waste, corn stover, cotton material and corona pre-treated cotton material. The maximum yield of reducing sugars was obtained on horsetail waste and corona pre-treated cotton material. Waste brewer's yeast Saccharomyces cerevisiae was successfully used for the production of bioethanol using horsetail waste hydrolysate and corona pre-treated cotton material hydrolysate. Conclusion The obtained results showed that bacterial strain CKS7 has a significant, still unexplored enzymatic potential that could be used to achieve a cleaner, environmental friendly and economically acceptable biofuel production. [GRAPHICS] .",
publisher = "Springer, Dordrecht",
journal = "Waste and Biomass Valorization",
title = "From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production",
pages = "174-165",
number = "1",
volume = "12",
doi = "10.1007/s12649-020-00960-3"
}

Mihajlovski, K., Buntić, A., Milić, M., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production. in Waste and Biomass Valorization
Springer, Dordrecht., 12(1), 165-174.
https://doi.org/10.1007/s12649-020-00960-3

Mihajlovski K, Buntić A, Milić M, Rajilić-Stojanović M, Dimitrijević-Branković S. From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production. in Waste and Biomass Valorization. 2021;12(1):165-174.
doi:10.1007/s12649-020-00960-3 .

Mihajlovski, Katarina, Buntić, Aneta, Milić, Marija, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production" in Waste and Biomass Valorization, 12, no. 1 (2021):165-174,
https://doi.org/10.1007/s12649-020-00960-3 . .

TY  - JOUR
AU  - Miljković, Miona
AU  - Lazić, Vesna
AU  - Davidović, Slađana
AU  - Milivojević, Ana
AU  - Papan, Jelena
AU  - Fernandes, Margarida M.
AU  - Lanceros-Mendez, Senentxu
AU  - Ahrenkiel, Scott Phillip
AU  - Nedeljković, Jovan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4351
AB  - The aim of this study was the development of a non-toxic, biosynthetic antimicrobial agent which selectively acts on only one type of microorganism, and preserves the microbiota. Antimicrobial performance of biosynthesized silver nanoparticles (Ag NPs) by horsetail (Equisetum arvense L.) extract was examined against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus, as well as yeasts Candida albicans and Saccharomyces boulardii. Also, the cytotoxicity of Ag NPs was examined toward pre-osteoblast cells. The synthetic conditions-concentration of extract, temperature, and pH-were optimized to prepare silver colloids with different particle size distributions and long-term stability. The obtained samples were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The smaller-sized Ag NPs ( 10-20 nm), prepared at a lower temperature (20 degrees C), showed better antimicrobial performance against E. coli compared to larger ones ( 40-60 nm), prepared at high temperature (100 degrees C). On the other hand, both samples did not display any toxic action against bacteria S. aureus, or yeasts C. albicans and S. boulardii. Non-cytotoxic behavior of Ag NPs toward pre-osteoblast cells was observed for the concentrations of silver  lt = 2.25 and  lt = 4.5 mg L-1 for 10-20 and 40-60 nm-sized Ag NPs, respectively. Biosynthesized Ag NPs by horsetail extract display selective toxic action against E. coli at the ecologically acceptable concentration level.
PB  - Springer, Dordrecht
T2  - Journal of Inorganic and Organometallic Polymers and Materials
T1  - Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli
EP  - 2607
IS  - 7
SP  - 2598
VL  - 30
DO  - 10.1007/s10904-019-01402-x
ER  - 

@article{
author = "Miljković, Miona and Lazić, Vesna and Davidović, Slađana and Milivojević, Ana and Papan, Jelena and Fernandes, Margarida M. and Lanceros-Mendez, Senentxu and Ahrenkiel, Scott Phillip and Nedeljković, Jovan",
year = "2020",
abstract = "The aim of this study was the development of a non-toxic, biosynthetic antimicrobial agent which selectively acts on only one type of microorganism, and preserves the microbiota. Antimicrobial performance of biosynthesized silver nanoparticles (Ag NPs) by horsetail (Equisetum arvense L.) extract was examined against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus, as well as yeasts Candida albicans and Saccharomyces boulardii. Also, the cytotoxicity of Ag NPs was examined toward pre-osteoblast cells. The synthetic conditions-concentration of extract, temperature, and pH-were optimized to prepare silver colloids with different particle size distributions and long-term stability. The obtained samples were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The smaller-sized Ag NPs ( 10-20 nm), prepared at a lower temperature (20 degrees C), showed better antimicrobial performance against E. coli compared to larger ones ( 40-60 nm), prepared at high temperature (100 degrees C). On the other hand, both samples did not display any toxic action against bacteria S. aureus, or yeasts C. albicans and S. boulardii. Non-cytotoxic behavior of Ag NPs toward pre-osteoblast cells was observed for the concentrations of silver  lt = 2.25 and  lt = 4.5 mg L-1 for 10-20 and 40-60 nm-sized Ag NPs, respectively. Biosynthesized Ag NPs by horsetail extract display selective toxic action against E. coli at the ecologically acceptable concentration level.",
publisher = "Springer, Dordrecht",
journal = "Journal of Inorganic and Organometallic Polymers and Materials",
title = "Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli",
pages = "2607-2598",
number = "7",
volume = "30",
doi = "10.1007/s10904-019-01402-x"
}

Miljković, M., Lazić, V., Davidović, S., Milivojević, A., Papan, J., Fernandes, M. M., Lanceros-Mendez, S., Ahrenkiel, S. P.,& Nedeljković, J.. (2020). Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli. in Journal of Inorganic and Organometallic Polymers and Materials
Springer, Dordrecht., 30(7), 2598-2607.
https://doi.org/10.1007/s10904-019-01402-x

Miljković M, Lazić V, Davidović S, Milivojević A, Papan J, Fernandes MM, Lanceros-Mendez S, Ahrenkiel SP, Nedeljković J. Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli. in Journal of Inorganic and Organometallic Polymers and Materials. 2020;30(7):2598-2607.
doi:10.1007/s10904-019-01402-x .

Miljković, Miona, Lazić, Vesna, Davidović, Slađana, Milivojević, Ana, Papan, Jelena, Fernandes, Margarida M., Lanceros-Mendez, Senentxu, Ahrenkiel, Scott Phillip, Nedeljković, Jovan, "Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli" in Journal of Inorganic and Organometallic Polymers and Materials, 30, no. 7 (2020):2598-2607,
https://doi.org/10.1007/s10904-019-01402-x . .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
AU  - Dimitrijević-Branković, Suzana
AU  - Davidović, Slađana
AU  - Radovanović, Neda
AU  - Delattre, Cédric
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4479
AB  - The use of polysaccharide-based materials presents an eco-friendly technological solution, by reducing dependence on fossil resources while reducing a product's carbon footprint, when compared to conventional plastic packaging materials. This review discusses the potential of polysaccharides as a raw material to produce multifunctional materials for food packaging applications. The covered areas include the recent innovations and properties of the polysaccharide-based materials. Emphasis is given to hemicelluloses, marine polysaccharides, and bacterial exopolysaccharides and their potential application in the latest trends of food packaging materials, including edible coatings, intelligent films, and thermo-insulated aerogel packaging.
PB  - MDPI, Basel
T2  - Molecules
T1  - Prospect of Polysaccharide-Based Materials as Advanced Food Packaging
IS  - 1
VL  - 25
DO  - 10.3390/molecules25010135
ER  - 

@article{
author = "Nešić, Aleksandra and Cabrera-Barjas, Gustavo and Dimitrijević-Branković, Suzana and Davidović, Slađana and Radovanović, Neda and Delattre, Cédric",
year = "2020",
abstract = "The use of polysaccharide-based materials presents an eco-friendly technological solution, by reducing dependence on fossil resources while reducing a product's carbon footprint, when compared to conventional plastic packaging materials. This review discusses the potential of polysaccharides as a raw material to produce multifunctional materials for food packaging applications. The covered areas include the recent innovations and properties of the polysaccharide-based materials. Emphasis is given to hemicelluloses, marine polysaccharides, and bacterial exopolysaccharides and their potential application in the latest trends of food packaging materials, including edible coatings, intelligent films, and thermo-insulated aerogel packaging.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Prospect of Polysaccharide-Based Materials as Advanced Food Packaging",
number = "1",
volume = "25",
doi = "10.3390/molecules25010135"
}

Nešić, A., Cabrera-Barjas, G., Dimitrijević-Branković, S., Davidović, S., Radovanović, N.,& Delattre, C.. (2020). Prospect of Polysaccharide-Based Materials as Advanced Food Packaging. in Molecules
MDPI, Basel., 25(1).
https://doi.org/10.3390/molecules25010135

Nešić A, Cabrera-Barjas G, Dimitrijević-Branković S, Davidović S, Radovanović N, Delattre C. Prospect of Polysaccharide-Based Materials as Advanced Food Packaging. in Molecules. 2020;25(1).
doi:10.3390/molecules25010135 .

Nešić, Aleksandra, Cabrera-Barjas, Gustavo, Dimitrijević-Branković, Suzana, Davidović, Slađana, Radovanović, Neda, Delattre, Cédric, "Prospect of Polysaccharide-Based Materials as Advanced Food Packaging" in Molecules, 25, no. 1 (2020),
https://doi.org/10.3390/molecules25010135 . .

TY  - JOUR
AU  - Bebić, Jelena
AU  - Banjanac, Katarina
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Marinković, Aleksandar
AU  - Bezbradica, Dejan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4384
AB  - This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oiyzae (Novozym 51003 (R) laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003 (R) laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU.mg(-1) proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity.
PB  - Chemical Industry Press Co Ltd, Beijing
T2  - Chinese Journal of Chemical Engineering
T1  - Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation
EP  - 1144
IS  - 4
SP  - 1136
VL  - 28
DO  - 10.1016/j.cjche.2019.12.025
ER  - 

@article{
author = "Bebić, Jelena and Banjanac, Katarina and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Marinković, Aleksandar and Bezbradica, Dejan",
year = "2020",
abstract = "This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oiyzae (Novozym 51003 (R) laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003 (R) laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU.mg(-1) proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity.",
publisher = "Chemical Industry Press Co Ltd, Beijing",
journal = "Chinese Journal of Chemical Engineering",
title = "Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation",
pages = "1144-1136",
number = "4",
volume = "28",
doi = "10.1016/j.cjche.2019.12.025"
}

Bebić, J., Banjanac, K., Ćorović, M., Milivojević, A., Simović, M., Marinković, A.,& Bezbradica, D.. (2020). Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation. in Chinese Journal of Chemical Engineering
Chemical Industry Press Co Ltd, Beijing., 28(4), 1136-1144.
https://doi.org/10.1016/j.cjche.2019.12.025

Bebić J, Banjanac K, Ćorović M, Milivojević A, Simović M, Marinković A, Bezbradica D. Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation. in Chinese Journal of Chemical Engineering. 2020;28(4):1136-1144.
doi:10.1016/j.cjche.2019.12.025 .

Bebić, Jelena, Banjanac, Katarina, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Marinković, Aleksandar, Bezbradica, Dejan, "Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation" in Chinese Journal of Chemical Engineering, 28, no. 4 (2020):1136-1144,
https://doi.org/10.1016/j.cjche.2019.12.025 . .

TY  - JOUR
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4494
AB  - Lipase-catalyzed transesterification of vitamin C by natural triglycerides is promising approach for cost-efficient synthesis of liposoluble food and cosmetic antioxidants. Nevertheless, application of these alternative acyl donors is insufficiently explored, despite of their low price, wide abundance and possibility for obtaining versatile products. Within current study, fatty acid ascorbyl esters were synthesized from vitamin C and natural triglycerides (lard, sunflower, coconut and linseed oil) in a process catalyzed by immobilized lipase Novozym (R) 435 and their controlled release was examined. High conversions were achieved at optimized conditions, even at high substrate concentrations, thus high concentrations of acsorbyl-ester were synthesized using these natural lipids. All ester mixtures exhibited very high capacity for scavenging of DPPH radicals, among which linseed oil derived esters, with ascorbyl linolenate as prevailing compound, were the most efficient (IC50 0.663 mu M), while coconut oil derived ester mixture (IC50 0.739 mu M), composed predominantly of molecules with medium side chains, was second best. Coconut oil derived esters were successfully incorporated in typical cosmetic formulations for controlled release of bioactive compounds - O/W emulsion and gel-emulsion. Franz cell diffusion study demonstrated that esters release from mixture and two carrier systems was formulation dependent and revealed decrease of effective diffusivities with ester side acyl chain length increase. According to calculated effective diffusivity coefficients, faster trans-membrane delivery of the same molecule was achieved from gel-emulsion comparing to O/W emulsion. Applied approach enabled highly efficient cost-saving production of fatty acid ascorbyl esters, which could find direct application in various lipophilic products.
PB  - Elsevier B.V.
T2  - Sustainable Chemistry and Pharmacy
T1  - Enzymatically derived oil-based L-ascorbyl esters: Synthesis, antioxidant properties and controlled release from cosmetic formulations
SP  - 100231
VL  - 15
DO  - 10.1016/j.scp.2020.100231
ER  - 

@article{
author = "Ćorović, Marija and Milivojević, Ana and Simović, Milica and Banjanac, Katarina and Pjanović, Rada and Bezbradica, Dejan",
year = "2020",
abstract = "Lipase-catalyzed transesterification of vitamin C by natural triglycerides is promising approach for cost-efficient synthesis of liposoluble food and cosmetic antioxidants. Nevertheless, application of these alternative acyl donors is insufficiently explored, despite of their low price, wide abundance and possibility for obtaining versatile products. Within current study, fatty acid ascorbyl esters were synthesized from vitamin C and natural triglycerides (lard, sunflower, coconut and linseed oil) in a process catalyzed by immobilized lipase Novozym (R) 435 and their controlled release was examined. High conversions were achieved at optimized conditions, even at high substrate concentrations, thus high concentrations of acsorbyl-ester were synthesized using these natural lipids. All ester mixtures exhibited very high capacity for scavenging of DPPH radicals, among which linseed oil derived esters, with ascorbyl linolenate as prevailing compound, were the most efficient (IC50 0.663 mu M), while coconut oil derived ester mixture (IC50 0.739 mu M), composed predominantly of molecules with medium side chains, was second best. Coconut oil derived esters were successfully incorporated in typical cosmetic formulations for controlled release of bioactive compounds - O/W emulsion and gel-emulsion. Franz cell diffusion study demonstrated that esters release from mixture and two carrier systems was formulation dependent and revealed decrease of effective diffusivities with ester side acyl chain length increase. According to calculated effective diffusivity coefficients, faster trans-membrane delivery of the same molecule was achieved from gel-emulsion comparing to O/W emulsion. Applied approach enabled highly efficient cost-saving production of fatty acid ascorbyl esters, which could find direct application in various lipophilic products.",
publisher = "Elsevier B.V.",
journal = "Sustainable Chemistry and Pharmacy",
title = "Enzymatically derived oil-based L-ascorbyl esters: Synthesis, antioxidant properties and controlled release from cosmetic formulations",
pages = "100231",
volume = "15",
doi = "10.1016/j.scp.2020.100231"
}

Ćorović, M., Milivojević, A., Simović, M., Banjanac, K., Pjanović, R.,& Bezbradica, D.. (2020). Enzymatically derived oil-based L-ascorbyl esters: Synthesis, antioxidant properties and controlled release from cosmetic formulations. in Sustainable Chemistry and Pharmacy
Elsevier B.V.., 15, 100231.
https://doi.org/10.1016/j.scp.2020.100231

Ćorović M, Milivojević A, Simović M, Banjanac K, Pjanović R, Bezbradica D. Enzymatically derived oil-based L-ascorbyl esters: Synthesis, antioxidant properties and controlled release from cosmetic formulations. in Sustainable Chemistry and Pharmacy. 2020;15:100231.
doi:10.1016/j.scp.2020.100231 .

Ćorović, Marija, Milivojević, Ana, Simović, Milica, Banjanac, Katarina, Pjanović, Rada, Bezbradica, Dejan, "Enzymatically derived oil-based L-ascorbyl esters: Synthesis, antioxidant properties and controlled release from cosmetic formulations" in Sustainable Chemistry and Pharmacy, 15 (2020):100231,
https://doi.org/10.1016/j.scp.2020.100231 . .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4536
AB  - Microbial hydrolytic enzymes are relevant biotechnological products that can be applied in various industries. In this study, we have tested the activity of hydrolytic enzymes of a newly isolated Hymenobacter sp. CKS3 strain and showed, for the first time, that members of Hymenobacter genus have still unexplored hydrolytic potential. Crude hydrolytic enzymes, produced by the strain CKS3 on a waste medium, were incorporated into a process of bioethanol production using waste bread. The conditions for bread hydrolysis were optimized using statistical design. Waste bread hydrolysate obtained under optimal conditions (100.73 h of hydrolysis, waste bread concentration 20.36% and agitation speed 200 rpm) contained 19.89 g/l of reducing sugars. A high performance liquid chromatography of hydrolyzed waste bread samples showed that the main components of the hydrolysate were dextrins, maltotriose, maltose and glucose. When using this substrate and waste baker's yeast for ethanol production under non-optimized conditions 1.73% of ethanol was produced. The results of this study showed that a newly isolated Hymenobacter sp. CKS3 can be utilized for enzymatic hydrolysis and bioethanol production in a process relying on waste materials. Furthermore, it was demonstrated that members of Hymenobacter genus have a significant and currently unexplored potential for bio-based industrial applications.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Renewable Energy
T1  - Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production
EP  - 633
SP  - 627
VL  - 152
DO  - 10.1016/j.renene.2020.01.101
ER  - 

@article{
author = "Mihajlovski, Katarina and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2020",
abstract = "Microbial hydrolytic enzymes are relevant biotechnological products that can be applied in various industries. In this study, we have tested the activity of hydrolytic enzymes of a newly isolated Hymenobacter sp. CKS3 strain and showed, for the first time, that members of Hymenobacter genus have still unexplored hydrolytic potential. Crude hydrolytic enzymes, produced by the strain CKS3 on a waste medium, were incorporated into a process of bioethanol production using waste bread. The conditions for bread hydrolysis were optimized using statistical design. Waste bread hydrolysate obtained under optimal conditions (100.73 h of hydrolysis, waste bread concentration 20.36% and agitation speed 200 rpm) contained 19.89 g/l of reducing sugars. A high performance liquid chromatography of hydrolyzed waste bread samples showed that the main components of the hydrolysate were dextrins, maltotriose, maltose and glucose. When using this substrate and waste baker's yeast for ethanol production under non-optimized conditions 1.73% of ethanol was produced. The results of this study showed that a newly isolated Hymenobacter sp. CKS3 can be utilized for enzymatic hydrolysis and bioethanol production in a process relying on waste materials. Furthermore, it was demonstrated that members of Hymenobacter genus have a significant and currently unexplored potential for bio-based industrial applications.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Renewable Energy",
title = "Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production",
pages = "633-627",
volume = "152",
doi = "10.1016/j.renene.2020.01.101"
}

Mihajlovski, K., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2020). Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production. in Renewable Energy
Pergamon-Elsevier Science Ltd, Oxford., 152, 627-633.
https://doi.org/10.1016/j.renene.2020.01.101

Mihajlovski K, Rajilić-Stojanović M, Dimitrijević-Branković S. Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production. in Renewable Energy. 2020;152:627-633.
doi:10.1016/j.renene.2020.01.101 .

Mihajlovski, Katarina, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production" in Renewable Energy, 152 (2020):627-633,
https://doi.org/10.1016/j.renene.2020.01.101 . .

TY  - JOUR
AU  - Buntić, Aneta
AU  - Milić, Marija
AU  - Stajković-Srbinović, Olivera S.
AU  - Rasulić, Nataša V.
AU  - Delić, Dušica I.
AU  - Mihajlovski, Katarina
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4075
AB  - This study has investigated the valorization of waste tobacco, as lignocellulosic biomass, for cellulase production by rhizobium belonging to genus Sinorhizobium. For the first time, Sinorhizobium meliloti strain 224 was used to produce cellulase (Avicelase and carboxymethyl cellulase) during the submerged and solid-state fermentation using tobacco waste as substrate. The effect of substrate chemical modification on enzymes production has been examined as well. The obtained optimal conditions for the maximum activity of both produced enzymes during submerged fermentation using response surface methodology were: 5 g/L of unmodified waste tobacco concentration, incubation time of 2 days and inoculum concentration of 9%. On the other hand, the use of 1 g of sodium hydroxide modified tobacco for the production of cellulase during solid-state fermentation with 10% inoculum, after 2 days of incubation at 28 degrees C, expressed the maximum Avicelase activity of 1.503 U/g and carboxymethyl cellulase activity of 1.615 U/g. In addition to its basic role in plant root colonization and the provision of nitrogen compounds, strain 224 can also be exploited to produce cellulases by bioconversion of plant waste.
PB  - Springer, New York
T2  - International Journal of Environmental Science and Technology
T1  - Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production
EP  - 5890
IS  - 10
SP  - 5881
VL  - 16
DO  - 10.1007/s13762-019-02230-9
ER  - 

@article{
author = "Buntić, Aneta and Milić, Marija and Stajković-Srbinović, Olivera S. and Rasulić, Nataša V. and Delić, Dušica I. and Mihajlovski, Katarina",
year = "2019",
abstract = "This study has investigated the valorization of waste tobacco, as lignocellulosic biomass, for cellulase production by rhizobium belonging to genus Sinorhizobium. For the first time, Sinorhizobium meliloti strain 224 was used to produce cellulase (Avicelase and carboxymethyl cellulase) during the submerged and solid-state fermentation using tobacco waste as substrate. The effect of substrate chemical modification on enzymes production has been examined as well. The obtained optimal conditions for the maximum activity of both produced enzymes during submerged fermentation using response surface methodology were: 5 g/L of unmodified waste tobacco concentration, incubation time of 2 days and inoculum concentration of 9%. On the other hand, the use of 1 g of sodium hydroxide modified tobacco for the production of cellulase during solid-state fermentation with 10% inoculum, after 2 days of incubation at 28 degrees C, expressed the maximum Avicelase activity of 1.503 U/g and carboxymethyl cellulase activity of 1.615 U/g. In addition to its basic role in plant root colonization and the provision of nitrogen compounds, strain 224 can also be exploited to produce cellulases by bioconversion of plant waste.",
publisher = "Springer, New York",
journal = "International Journal of Environmental Science and Technology",
title = "Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production",
pages = "5890-5881",
number = "10",
volume = "16",
doi = "10.1007/s13762-019-02230-9"
}

Buntić, A., Milić, M., Stajković-Srbinović, O. S., Rasulić, N. V., Delić, D. I.,& Mihajlovski, K.. (2019). Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production. in International Journal of Environmental Science and Technology
Springer, New York., 16(10), 5881-5890.
https://doi.org/10.1007/s13762-019-02230-9

Buntić A, Milić M, Stajković-Srbinović OS, Rasulić NV, Delić DI, Mihajlovski K. Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production. in International Journal of Environmental Science and Technology. 2019;16(10):5881-5890.
doi:10.1007/s13762-019-02230-9 .

Buntić, Aneta, Milić, Marija, Stajković-Srbinović, Olivera S., Rasulić, Nataša V., Delić, Dušica I., Mihajlovski, Katarina, "Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production" in International Journal of Environmental Science and Technology, 16, no. 10 (2019):5881-5890,
https://doi.org/10.1007/s13762-019-02230-9 . .

TY  - JOUR
AU  - Buntić, Aneta
AU  - Milić, Marija
AU  - Antonović, Dušan
AU  - Šiler-Marinković, Slavica
AU  - Dimitrijević-Branković, Suzana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4129
AB  - The use of the microwave-activated sugar beet pulp as an alternative adsorbent material in combination with biological processes has been applied for the colored wastewater treatment. The maximum adsorption capacity of the crystal violet dye has been achieved relatively fast, during 315 s, with no dependence of the pH, when the adsorbent mass was 15 and 0.2 g/L of the initial dye concentration. The adsorption equilibrium data fully complied with the Temkin isotherm. The afterward biological decontamination of the adsorbed dye was related to the biodegradable potential of Streptomyces microflavus CKS6 cells. The microorganism revealed strong affinity toward the dye and minimal nutritional requirements for the metabolic activity expressions. Total dye decomposition occurred after 9 h, whereby lignin peroxidase enzyme has been recognized to be mainly involved in the process. According to the phytotoxicity analysis, no harmful residues were found in the decolorized working medium.
PB  - Desalination Publ, Hopkinton
T2  - Desalination and Water Treatment
T1  - Implementation of integrated adsorption and biological process in wastewater treatment for permanent dye removal and its subsequent decontamination
EP  - 382
SP  - 372
VL  - 169
DO  - 10.5004/dwt.2019.24677
ER  - 

@article{
author = "Buntić, Aneta and Milić, Marija and Antonović, Dušan and Šiler-Marinković, Slavica and Dimitrijević-Branković, Suzana",
year = "2019",
abstract = "The use of the microwave-activated sugar beet pulp as an alternative adsorbent material in combination with biological processes has been applied for the colored wastewater treatment. The maximum adsorption capacity of the crystal violet dye has been achieved relatively fast, during 315 s, with no dependence of the pH, when the adsorbent mass was 15 and 0.2 g/L of the initial dye concentration. The adsorption equilibrium data fully complied with the Temkin isotherm. The afterward biological decontamination of the adsorbed dye was related to the biodegradable potential of Streptomyces microflavus CKS6 cells. The microorganism revealed strong affinity toward the dye and minimal nutritional requirements for the metabolic activity expressions. Total dye decomposition occurred after 9 h, whereby lignin peroxidase enzyme has been recognized to be mainly involved in the process. According to the phytotoxicity analysis, no harmful residues were found in the decolorized working medium.",
publisher = "Desalination Publ, Hopkinton",
journal = "Desalination and Water Treatment",
title = "Implementation of integrated adsorption and biological process in wastewater treatment for permanent dye removal and its subsequent decontamination",
pages = "382-372",
volume = "169",
doi = "10.5004/dwt.2019.24677"
}

Buntić, A., Milić, M., Antonović, D., Šiler-Marinković, S.,& Dimitrijević-Branković, S.. (2019). Implementation of integrated adsorption and biological process in wastewater treatment for permanent dye removal and its subsequent decontamination. in Desalination and Water Treatment
Desalination Publ, Hopkinton., 169, 372-382.
https://doi.org/10.5004/dwt.2019.24677

Buntić A, Milić M, Antonović D, Šiler-Marinković S, Dimitrijević-Branković S. Implementation of integrated adsorption and biological process in wastewater treatment for permanent dye removal and its subsequent decontamination. in Desalination and Water Treatment. 2019;169:372-382.
doi:10.5004/dwt.2019.24677 .

Buntić, Aneta, Milić, Marija, Antonović, Dušan, Šiler-Marinković, Slavica, Dimitrijević-Branković, Suzana, "Implementation of integrated adsorption and biological process in wastewater treatment for permanent dye removal and its subsequent decontamination" in Desalination and Water Treatment, 169 (2019):372-382,
https://doi.org/10.5004/dwt.2019.24677 . .

TY  - JOUR
AU  - Buntić, Aneta
AU  - Stajković-Srbinović, Olivera S.
AU  - Delić, Dušica I.
AU  - Dimitrijević-Branković, Suzana
AU  - Milić, Marija
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4311
AB  - Agricultural by-products are generated in large amounts in various industries, creating a serious disposal problem. Valorisation of tobacco waste for the extraction of value-added compounds and the production of enzymes could reduce both the problems of its disposal and the costs of cellulase production. Hitherto, there have been no reported studies concerning the utilization of tobacco residues (after extraction of polyphenols and nicotine, and fermentation pre-treatment by Streptomyces fulvissimus CKS7) for the production of cellulase (CMCase and.vicelase) by Paenibacillus chitinolyticus CKS1. The optimal conditions for the polyphenols and nicotine extraction process were obtained using the response surface methodology: 60 s extraction time in water and 30 mL g(-1) liquid/solid ratio. After the applied bacterial fermentation as a pre-treatment of tobacco residues, using Paenibacillus species, the extraction of polyphenols decreased by up to 10 %, while the extraction of nicotine increased by up to 35 %. Afterward, the maximum of cellulase activities (CMCase of 0.878 U g(-1) and avicelase of 1.417 U g(-1)) were achieved using of the strain CKS1.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment
EP  - 140
IS  - 2
SP  - 129
VL  - 84
DO  - 10.2298/JSC180802114B
ER  - 

@article{
author = "Buntić, Aneta and Stajković-Srbinović, Olivera S. and Delić, Dušica I. and Dimitrijević-Branković, Suzana and Milić, Marija",
year = "2019",
abstract = "Agricultural by-products are generated in large amounts in various industries, creating a serious disposal problem. Valorisation of tobacco waste for the extraction of value-added compounds and the production of enzymes could reduce both the problems of its disposal and the costs of cellulase production. Hitherto, there have been no reported studies concerning the utilization of tobacco residues (after extraction of polyphenols and nicotine, and fermentation pre-treatment by Streptomyces fulvissimus CKS7) for the production of cellulase (CMCase and.vicelase) by Paenibacillus chitinolyticus CKS1. The optimal conditions for the polyphenols and nicotine extraction process were obtained using the response surface methodology: 60 s extraction time in water and 30 mL g(-1) liquid/solid ratio. After the applied bacterial fermentation as a pre-treatment of tobacco residues, using Paenibacillus species, the extraction of polyphenols decreased by up to 10 %, while the extraction of nicotine increased by up to 35 %. Afterward, the maximum of cellulase activities (CMCase of 0.878 U g(-1) and avicelase of 1.417 U g(-1)) were achieved using of the strain CKS1.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment",
pages = "140-129",
number = "2",
volume = "84",
doi = "10.2298/JSC180802114B"
}

Buntić, A., Stajković-Srbinović, O. S., Delić, D. I., Dimitrijević-Branković, S.,& Milić, M.. (2019). The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(2), 129-140.
https://doi.org/10.2298/JSC180802114B

Buntić A, Stajković-Srbinović OS, Delić DI, Dimitrijević-Branković S, Milić M. The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment. in Journal of the Serbian Chemical Society. 2019;84(2):129-140.
doi:10.2298/JSC180802114B .

Buntić, Aneta, Stajković-Srbinović, Olivera S., Delić, Dušica I., Dimitrijević-Branković, Suzana, Milić, Marija, "The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment" in Journal of the Serbian Chemical Society, 84, no. 2 (2019):129-140,
https://doi.org/10.2298/JSC180802114B . .

TY  - CONF
AU  - Buntić, Aneta
AU  - Stajković-Srbinović, Olivera
AU  - Milić, Marija
AU  - Dubljanin, Tijana
AU  - Kuzmanović, Đorđe
AU  - Knežević, Magdalena
AU  - Delić, Dušica
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6415
AB  - Miscanthus × giganteus, as a lignocellulosic material, consists of about 30%
xylan and represents a good basis for research as a possible raw material in
the production of xylanases. Among the soil bacteria, rhizobium is rarely
investigated as an enzyme producer. For the first time, Sinorhizobium
meliloti strain 207 was used to obtain xylanase during submerged and solid
fermentation using miscanthus biomass as a substrate. During submerged
fermentation (28°C), the maximum xylanase activity was achieved after 48 h
with 10% inoculum and H2SO4 substrate modification. The maximum
xylanase activity of 1.215 U/mL was obtained during solid-state
fermentation (28°C) by using also H2SO4 modified miscanthus biomass
which was moistened with distillate water. The crude enzyme, produced by
strain 207, could be further used in eco-friendly processes of lignocellulose
material bioconversion to useful products.
PB  - The Balkans Scientific Center of the Russian Academy of Natural Sciences
C3  - 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection
T1  - Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti
EP  - 163
SP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6415
ER  - 

@conference{
author = "Buntić, Aneta and Stajković-Srbinović, Olivera and Milić, Marija and Dubljanin, Tijana and Kuzmanović, Đorđe and Knežević, Magdalena and Delić, Dušica",
year = "2019",
abstract = "Miscanthus × giganteus, as a lignocellulosic material, consists of about 30%
xylan and represents a good basis for research as a possible raw material in
the production of xylanases. Among the soil bacteria, rhizobium is rarely
investigated as an enzyme producer. For the first time, Sinorhizobium
meliloti strain 207 was used to obtain xylanase during submerged and solid
fermentation using miscanthus biomass as a substrate. During submerged
fermentation (28°C), the maximum xylanase activity was achieved after 48 h
with 10% inoculum and H2SO4 substrate modification. The maximum
xylanase activity of 1.215 U/mL was obtained during solid-state
fermentation (28°C) by using also H2SO4 modified miscanthus biomass
which was moistened with distillate water. The crude enzyme, produced by
strain 207, could be further used in eco-friendly processes of lignocellulose
material bioconversion to useful products.",
publisher = "The Balkans Scientific Center of the Russian Academy of Natural Sciences",
journal = "1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection",
title = "Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti",
pages = "163-151",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6415"
}

Buntić, A., Stajković-Srbinović, O., Milić, M., Dubljanin, T., Kuzmanović, Đ., Knežević, M.,& Delić, D.. (2019). Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti. in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection
The Balkans Scientific Center of the Russian Academy of Natural Sciences., 151-163.
https://hdl.handle.net/21.15107/rcub_technorep_6415

Buntić A, Stajković-Srbinović O, Milić M, Dubljanin T, Kuzmanović Đ, Knežević M, Delić D. Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti. in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection. 2019;:151-163.
https://hdl.handle.net/21.15107/rcub_technorep_6415 .

Buntić, Aneta, Stajković-Srbinović, Olivera, Milić, Marija, Dubljanin, Tijana, Kuzmanović, Đorđe, Knežević, Magdalena, Delić, Dušica, "Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti" in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection (2019):151-163,
https://hdl.handle.net/21.15107/rcub_technorep_6415 .

TY  - PAT
AU  - Mihajlovski, Katarina
AU  - Simović, Milica
AU  - Dimitrijević-Branković, Suzana
AU  - Rajilić-Stojanović, Mirjana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6460
PB  - Zavod za intelektualnu svojinu, Glasnik intelektualne svojine, Beograd
T2  - Glasnik intelektualne svojine, Beograd, 2019/10
T1  - Novi bakterijski soj Hymenobacter sp. CKS3 za proizvodnju enzima amilaza i primena ovih enzima
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6460
ER  - 

@misc{
author = "Mihajlovski, Katarina and Simović, Milica and Dimitrijević-Branković, Suzana and Rajilić-Stojanović, Mirjana",
year = "2019",
publisher = "Zavod za intelektualnu svojinu, Glasnik intelektualne svojine, Beograd",
journal = "Glasnik intelektualne svojine, Beograd, 2019/10",
title = "Novi bakterijski soj Hymenobacter sp. CKS3 za proizvodnju enzima amilaza i primena ovih enzima",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6460"
}

Mihajlovski, K., Simović, M., Dimitrijević-Branković, S.,& Rajilić-Stojanović, M.. (2019). Novi bakterijski soj Hymenobacter sp. CKS3 za proizvodnju enzima amilaza i primena ovih enzima. in Glasnik intelektualne svojine, Beograd, 2019/10
Zavod za intelektualnu svojinu, Glasnik intelektualne svojine, Beograd..
https://hdl.handle.net/21.15107/rcub_technorep_6460

Mihajlovski K, Simović M, Dimitrijević-Branković S, Rajilić-Stojanović M. Novi bakterijski soj Hymenobacter sp. CKS3 za proizvodnju enzima amilaza i primena ovih enzima. in Glasnik intelektualne svojine, Beograd, 2019/10. 2019;.
https://hdl.handle.net/21.15107/rcub_technorep_6460 .

Mihajlovski, Katarina, Simović, Milica, Dimitrijević-Branković, Suzana, Rajilić-Stojanović, Mirjana, "Novi bakterijski soj Hymenobacter sp. CKS3 za proizvodnju enzima amilaza i primena ovih enzima" in Glasnik intelektualne svojine, Beograd, 2019/10 (2019),
https://hdl.handle.net/21.15107/rcub_technorep_6460 .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Gordić, Milan V.
AU  - Petrović, Jelena
AU  - Pavlović, Vladimir B.
AU  - Mitrić, Miodrag
AU  - Nešić, Aleksandra
AU  - Dimitrijević-Branković, Suzana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4054
AB  - New agar-based composite films with increasing Cu-carbonate and Cu-phosphate mineral phase content were prepared by in situ mineralization and solvent casting method. SEM and optical analysis revealed that Cu-carbonate phase had better compatibility with agar matrix than Cu-phosphate phase. Incorporation of both mineral phases improved mechanical and water vapor barrier properties of the obtained mineralized films, in concentration dependent manner. When 5 mM of carbonate precursor was incorporated into agar matrix, mechanical resistance was enchanced for 44% and water vapor barrier property for 40%. The release of Cu (II) was higher in acidic conditions for both mineralized composites and remained in the range of specific release limits for this metal. In addition, both mineralized composite films exhibited distinctive antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Overall, the Cu-carbonate and Cu-phosphate mineralized agar films showed potential to be used for food packaging materials, agriculture or medical purposes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - European Polymer Journal
T1  - Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase
EP  - 358
SP  - 352
VL  - 119
DO  - 10.1016/j.eurpolymj.2019.08.004
ER  - 

@article{
author = "Radovanović, Neda and Malagurski, Ivana and Lević, Steva and Gordić, Milan V. and Petrović, Jelena and Pavlović, Vladimir B. and Mitrić, Miodrag and Nešić, Aleksandra and Dimitrijević-Branković, Suzana",
year = "2019",
abstract = "New agar-based composite films with increasing Cu-carbonate and Cu-phosphate mineral phase content were prepared by in situ mineralization and solvent casting method. SEM and optical analysis revealed that Cu-carbonate phase had better compatibility with agar matrix than Cu-phosphate phase. Incorporation of both mineral phases improved mechanical and water vapor barrier properties of the obtained mineralized films, in concentration dependent manner. When 5 mM of carbonate precursor was incorporated into agar matrix, mechanical resistance was enchanced for 44% and water vapor barrier property for 40%. The release of Cu (II) was higher in acidic conditions for both mineralized composites and remained in the range of specific release limits for this metal. In addition, both mineralized composite films exhibited distinctive antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Overall, the Cu-carbonate and Cu-phosphate mineralized agar films showed potential to be used for food packaging materials, agriculture or medical purposes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "European Polymer Journal",
title = "Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase",
pages = "358-352",
volume = "119",
doi = "10.1016/j.eurpolymj.2019.08.004"
}

Radovanović, N., Malagurski, I., Lević, S., Gordić, M. V., Petrović, J., Pavlović, V. B., Mitrić, M., Nešić, A.,& Dimitrijević-Branković, S.. (2019). Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase. in European Polymer Journal
Pergamon-Elsevier Science Ltd, Oxford., 119, 352-358.
https://doi.org/10.1016/j.eurpolymj.2019.08.004

Radovanović N, Malagurski I, Lević S, Gordić MV, Petrović J, Pavlović VB, Mitrić M, Nešić A, Dimitrijević-Branković S. Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase. in European Polymer Journal. 2019;119:352-358.
doi:10.1016/j.eurpolymj.2019.08.004 .

Radovanović, Neda, Malagurski, Ivana, Lević, Steva, Gordić, Milan V., Petrović, Jelena, Pavlović, Vladimir B., Mitrić, Miodrag, Nešić, Aleksandra, Dimitrijević-Branković, Suzana, "Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase" in European Polymer Journal, 119 (2019):352-358,
https://doi.org/10.1016/j.eurpolymj.2019.08.004 . .

TY  - JOUR
AU  - Davidović, Slađana
AU  - Lazić, Vesna M.
AU  - Miljković, Miona
AU  - Gordić, Milan V.
AU  - Sekulić, Milica
AU  - Marinović-Cincović, Milena
AU  - Ratnayake, Ishara S.
AU  - Ahrenkiel, Scott Phillip
AU  - Nedeljković, Jovan
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4081
AB  - The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (  lt  2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10-20 nm silver nanoparticles (Ag NPs) within the agar-agar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agar-agar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inorganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureus was observed for all agar-silver films, while satisfactory results were observed for Gram-negative bacteria Pseudomonas aeruginosa (  gt = 99.6%). The release of Ag+ ions is suppressed by the increase of the concentration of Mg2+ ions and it was found to be significantly smaller (  lt = 0.24 ppm) than the harmful ecological level (1 ppm).
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions
VL  - 224
DO  - 10.1016/j.carbpol.2019.115187
ER  - 

@article{
author = "Davidović, Slađana and Lazić, Vesna M. and Miljković, Miona and Gordić, Milan V. and Sekulić, Milica and Marinović-Cincović, Milena and Ratnayake, Ishara S. and Ahrenkiel, Scott Phillip and Nedeljković, Jovan",
year = "2019",
abstract = "The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (  lt  2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10-20 nm silver nanoparticles (Ag NPs) within the agar-agar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agar-agar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inorganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureus was observed for all agar-silver films, while satisfactory results were observed for Gram-negative bacteria Pseudomonas aeruginosa (  gt = 99.6%). The release of Ag+ ions is suppressed by the increase of the concentration of Mg2+ ions and it was found to be significantly smaller (  lt = 0.24 ppm) than the harmful ecological level (1 ppm).",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions",
volume = "224",
doi = "10.1016/j.carbpol.2019.115187"
}

Davidović, S., Lazić, V. M., Miljković, M., Gordić, M. V., Sekulić, M., Marinović-Cincović, M., Ratnayake, I. S., Ahrenkiel, S. P.,& Nedeljković, J.. (2019). Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 224.
https://doi.org/10.1016/j.carbpol.2019.115187

Davidović S, Lazić VM, Miljković M, Gordić MV, Sekulić M, Marinović-Cincović M, Ratnayake IS, Ahrenkiel SP, Nedeljković J. Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions. in Carbohydrate Polymers. 2019;224.
doi:10.1016/j.carbpol.2019.115187 .

Davidović, Slađana, Lazić, Vesna M., Miljković, Miona, Gordić, Milan V., Sekulić, Milica, Marinović-Cincović, Milena, Ratnayake, Ishara S., Ahrenkiel, Scott Phillip, Nedeljković, Jovan, "Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions" in Carbohydrate Polymers, 224 (2019),
https://doi.org/10.1016/j.carbpol.2019.115187 . .

TY  - THES
AU  - Davidović, Slađana
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7279
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:21025/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51886095
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4686
AB  - The scope of this doctoral dissertation is the isolation of dextran producing lactic acid bacteria from the water kefir grain and testing the possibility of the obtained dextran for silver nanoparticles synthesis and edible film's production...
AB  - Predmet istraživanja ove doktorske disertacije je izolovanje bakterija mlečne kiseline iz zrna vodenog kefira, u cilju sinteze dekstrana, kao i ispitivanje mogućnosti primene dobijenog dekstrana za sintezu nanočestica srebra i proizvodnju jestivih filmova...
PB  - Univerzitet u Beogradu, Tehnološko-metalurški fakultet
T1  - Application of dextran from lactic acid bacteria for silver nanoparticles synthesis and edible coating production
T1  - Primena dekstrana iz bakterija mlečne kiseline za sintezu nanočestica srebra i proizvodnju jestivih filmova
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4686
ER  - 

@phdthesis{
author = "Davidović, Slađana",
year = "2019",
abstract = "The scope of this doctoral dissertation is the isolation of dextran producing lactic acid bacteria from the water kefir grain and testing the possibility of the obtained dextran for silver nanoparticles synthesis and edible film's production..., Predmet istraživanja ove doktorske disertacije je izolovanje bakterija mlečne kiseline iz zrna vodenog kefira, u cilju sinteze dekstrana, kao i ispitivanje mogućnosti primene dobijenog dekstrana za sintezu nanočestica srebra i proizvodnju jestivih filmova...",
publisher = "Univerzitet u Beogradu, Tehnološko-metalurški fakultet",
title = "Application of dextran from lactic acid bacteria for silver nanoparticles synthesis and edible coating production, Primena dekstrana iz bakterija mlečne kiseline za sintezu nanočestica srebra i proizvodnju jestivih filmova",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4686"
}

Davidović, S.. (2019). Application of dextran from lactic acid bacteria for silver nanoparticles synthesis and edible coating production. 
Univerzitet u Beogradu, Tehnološko-metalurški fakultet..
https://hdl.handle.net/21.15107/rcub_technorep_4686

Davidović S. Application of dextran from lactic acid bacteria for silver nanoparticles synthesis and edible coating production. 2019;.
https://hdl.handle.net/21.15107/rcub_technorep_4686 .

Davidović, Slađana, "Application of dextran from lactic acid bacteria for silver nanoparticles synthesis and edible coating production" (2019),
https://hdl.handle.net/21.15107/rcub_technorep_4686 .

TY  - JOUR
AU  - Simović, Milica
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Banjanac, Katarina
AU  - Bezbradica, Dejan
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4045
AB  - Whey represents a significant dairy industry by-product that has recently received due attention based on the rich nutrient composition and significant transformation potential. Hereby, we investigated a possibility of whey lactose bioconversion into prebiotic compounds, galacto-oligosaccharides (GOS) using beta-galactosidase transgalactosylation activity. The results showed that whey could be successfully used for GOS synthesis, since the highest GOS concentration (around 62 g L-1) was obtained batchwise using 40% (w/w) sweet whey powder solution under optimum conditions (50 degrees C, pH 4.5). Nevertheless, an efficient immobilized preparation using methacrylic Lifetech ECR8409 immobilization carrier was developed, enabling additional process improvement and ensuring at least 4 reaction cycles with unchanged yields and 2.5- fold enhanced productivity in comparison to the soluble enzyme. Therefore, this study provides a valuable contribution to the efficient and economical valorization of whey, which can be further on utilized as functional food and feed constituent.
PB  - Wiley, Hoboken
T2  - International Journal of Food Science and Technology
T1  - Whey valorization using transgalactosylation activity of immobilized beta-galactosidase
EP  - 3082
IS  - 11
SP  - 3074
VL  - 54
DO  - 10.1111/ijfs.14222
ER  - 

@article{
author = "Simović, Milica and Milivojević, Ana and Ćorović, Marija and Banjanac, Katarina and Bezbradica, Dejan",
year = "2019",
abstract = "Whey represents a significant dairy industry by-product that has recently received due attention based on the rich nutrient composition and significant transformation potential. Hereby, we investigated a possibility of whey lactose bioconversion into prebiotic compounds, galacto-oligosaccharides (GOS) using beta-galactosidase transgalactosylation activity. The results showed that whey could be successfully used for GOS synthesis, since the highest GOS concentration (around 62 g L-1) was obtained batchwise using 40% (w/w) sweet whey powder solution under optimum conditions (50 degrees C, pH 4.5). Nevertheless, an efficient immobilized preparation using methacrylic Lifetech ECR8409 immobilization carrier was developed, enabling additional process improvement and ensuring at least 4 reaction cycles with unchanged yields and 2.5- fold enhanced productivity in comparison to the soluble enzyme. Therefore, this study provides a valuable contribution to the efficient and economical valorization of whey, which can be further on utilized as functional food and feed constituent.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Food Science and Technology",
title = "Whey valorization using transgalactosylation activity of immobilized beta-galactosidase",
pages = "3082-3074",
number = "11",
volume = "54",
doi = "10.1111/ijfs.14222"
}

Simović, M., Milivojević, A., Ćorović, M., Banjanac, K.,& Bezbradica, D.. (2019). Whey valorization using transgalactosylation activity of immobilized beta-galactosidase. in International Journal of Food Science and Technology
Wiley, Hoboken., 54(11), 3074-3082.
https://doi.org/10.1111/ijfs.14222

Simović M, Milivojević A, Ćorović M, Banjanac K, Bezbradica D. Whey valorization using transgalactosylation activity of immobilized beta-galactosidase. in International Journal of Food Science and Technology. 2019;54(11):3074-3082.
doi:10.1111/ijfs.14222 .

Simović, Milica, Milivojević, Ana, Ćorović, Marija, Banjanac, Katarina, Bezbradica, Dejan, "Whey valorization using transgalactosylation activity of immobilized beta-galactosidase" in International Journal of Food Science and Technology, 54, no. 11 (2019):3074-3082,
https://doi.org/10.1111/ijfs.14222 . .

TY  - JOUR
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Blagojević, Stevan N.
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4158
AB  - The present work describes application of natural triglycerides for lipase-catalyzed esterification of structurally diverse flavonoids-esculin, naringin, and phloridzin. Ester mixtures with laurate, linolenate, or linoleate as prevailing products were obtained in reactions with coconut, linseed, and sunflower oils, respectively. By using response surface methodology, the influences of key experimental factors-reaction time, substrates, and enzyme concentrations-for transesterification of esculin with linseed oil were investigated. High molar conversion (78.5%), product yield (19.13 mg/mL), and specific yield (2.83 mmol/g(enzyme)) were achieved at optimized conditions. Determined optimums were successfully applied in esculin transesterification with other two oils. Furthermore, esculin ester mixtures were incorporated in gel emulsions, and their effect on transepidermal water loss (TEWL) as well as skin sensitivity was examined. Obtained results have shown significant hydration effect and good dermatological compatibility without irritating effects of all examined preparations containing oil-derived esculin esters, making them very prospective for application in skin care products.
PB  - Amer Chemical Soc, Washington
T2  - Industrial & Engineering Chemistry Research
T1  - Novel Approach for Flavonoid Esters Production: Statistically Optimized Enzymatic Synthesis Using Natural Oils and Application in Cosmetics
EP  - 3649
IS  - 9
SP  - 3640
VL  - 58
DO  - 10.1021/acs.iecr.8b06113
ER  - 

@article{
author = "Milivojević, Ana and Ćorović, Marija and Simović, Milica and Banjanac, Katarina and Blagojević, Stevan N. and Pjanović, Rada and Bezbradica, Dejan",
year = "2019",
abstract = "The present work describes application of natural triglycerides for lipase-catalyzed esterification of structurally diverse flavonoids-esculin, naringin, and phloridzin. Ester mixtures with laurate, linolenate, or linoleate as prevailing products were obtained in reactions with coconut, linseed, and sunflower oils, respectively. By using response surface methodology, the influences of key experimental factors-reaction time, substrates, and enzyme concentrations-for transesterification of esculin with linseed oil were investigated. High molar conversion (78.5%), product yield (19.13 mg/mL), and specific yield (2.83 mmol/g(enzyme)) were achieved at optimized conditions. Determined optimums were successfully applied in esculin transesterification with other two oils. Furthermore, esculin ester mixtures were incorporated in gel emulsions, and their effect on transepidermal water loss (TEWL) as well as skin sensitivity was examined. Obtained results have shown significant hydration effect and good dermatological compatibility without irritating effects of all examined preparations containing oil-derived esculin esters, making them very prospective for application in skin care products.",
publisher = "Amer Chemical Soc, Washington",
journal = "Industrial & Engineering Chemistry Research",
title = "Novel Approach for Flavonoid Esters Production: Statistically Optimized Enzymatic Synthesis Using Natural Oils and Application in Cosmetics",
pages = "3649-3640",
number = "9",
volume = "58",
doi = "10.1021/acs.iecr.8b06113"
}

Milivojević, A., Ćorović, M., Simović, M., Banjanac, K., Blagojević, S. N., Pjanović, R.,& Bezbradica, D.. (2019). Novel Approach for Flavonoid Esters Production: Statistically Optimized Enzymatic Synthesis Using Natural Oils and Application in Cosmetics. in Industrial & Engineering Chemistry Research
Amer Chemical Soc, Washington., 58(9), 3640-3649.
https://doi.org/10.1021/acs.iecr.8b06113

Milivojević A, Ćorović M, Simović M, Banjanac K, Blagojević SN, Pjanović R, Bezbradica D. Novel Approach for Flavonoid Esters Production: Statistically Optimized Enzymatic Synthesis Using Natural Oils and Application in Cosmetics. in Industrial & Engineering Chemistry Research. 2019;58(9):3640-3649.
doi:10.1021/acs.iecr.8b06113 .

Milivojević, Ana, Ćorović, Marija, Simović, Milica, Banjanac, Katarina, Blagojević, Stevan N., Pjanović, Rada, Bezbradica, Dejan, "Novel Approach for Flavonoid Esters Production: Statistically Optimized Enzymatic Synthesis Using Natural Oils and Application in Cosmetics" in Industrial & Engineering Chemistry Research, 58, no. 9 (2019):3640-3649,
https://doi.org/10.1021/acs.iecr.8b06113 . .

TY  - JOUR
AU  - Ćorović, Marija
AU  - Simović, Milica
AU  - Milivojević, Ana
AU  - Banjanac, Katarina
AU  - Katić, Katarina D.
AU  - Bezbradica, Dejan
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4299
AB  - Cellulases are enzymes which catalyse cellulose hydrolysis and are widely used in various industry branches. Lately, their application in treatment of different agroindustrial waste materials which could serve for fuel production is being extensively explored. In order to increase their stability and cost-effectiveness of their usage, application of their immobilized forms are preferred over free enzymes. Hereby, we tested eight different LifetechTM immobilization supports differing in polarity, porosity and functional groups as carriers for Asspergillus Niger cellulase immobilization. Most promising carrier was methacrylate based, with primary amino groups, C6 "space arm" and pores with diameter of 60-120 nm - LifetechTM ECR8409F. For this support, most important immobilization parameters were investigated and after 3 h at pH 6 with initial protein concentration of 23.3 mg/g support immobilized cellulase with 406 IU/g (with carboxymethyl cellulose as a substrate) was obtained. This preparation was successfully applied in the hydrolysis of lignocellulosic fraction of sunflower seed meal, which is widely available byproduct of sunflower seed meal fractionation for protein-rich fractions production. Initial reaction rates and yields of reducing sugars were unchanged comparing to free enzyme, indicating that there were no significant diffusion limitations for substrate to approach active sites of A. Niger cellulase molecules immobilized onto LifetechTM ECR8409F support.
AB  - Celulaze su enzimi koji katalizuju hidrolizu celuloze i u širokoj su primeni u brojnim granama industrije. U poslednje vreme, mogućnost njihove primene u tretiranju različitih opadnih agroindustrijskih sirovina u cilju dobijanja biogoriva intenzivno se istražuje. Da bi se povećale stabilnost i ekonomičnost njihove primene, potrebno je umesto slobodnog enzima koristiti imobilisane forme. U ovom istraživanju osam LifetechTM nosača različitih polarnosti, poroznosti i funkcionalnih grupa. testirano je za imobilizaciju celulaze producenta Aspergillus Niger. Najpogodniji nosač bio je na bazi metakrilata, sa primarnim amino grupama, C6 "dugom nožicom" i porama prečnika 60-120 nm - LifetechTM ECR8409F. Za ovaj nosač određeni su najznačajniji uslovi imobilizacije i nakon 3 sata na pH 6, pri početnoj koncentraciji proteina od 23,3 mg/g nosača dobijena je imobilisana celulaza aktivnosti 406 IU/g (sa karboksi metil celulozom kao supstratom). Ovaj preparat uspešno je primenjen u reakciji hidrolize lignocelulozne frakcije suncokretove sačme, koja prestavlja nusproizvod frakcionisanja suncokretove sačme pri dobijanju frakcija bogatih proteinima. Početne brzine reakcije i prinosi redukujućih šećera bili su nepromenjeni u odnosu na slobodan enzim, ukazujući da nije bilo značajnog uticaja difuzionih limitacija pri prilasku supstrata aktivnim centrima molekula A. Niger celulaze imobilisane na LifetechTM ECR8409F nosač.
PB  - Institute for Food Technology, Novi Sad
T2  - Food and Feed Research
T1  - Immobilization of Aspergillus Niger cellulase onto Lifetech TM carriers and its application in the hydrolysis of sunflower seed meal lignocellulosic fraction
T1  - Imobilizacija Aspergillus Niger celulaze na Lifetech TM nosače i primena u reakciji hidrolize lignocelulozne frakcije suncokretove sačme
EP  - 169
IS  - 2
SP  - 161
VL  - 46
DO  - 10.5937/FFR1902161C
ER  - 

@article{
author = "Ćorović, Marija and Simović, Milica and Milivojević, Ana and Banjanac, Katarina and Katić, Katarina D. and Bezbradica, Dejan",
year = "2019",
abstract = "Cellulases are enzymes which catalyse cellulose hydrolysis and are widely used in various industry branches. Lately, their application in treatment of different agroindustrial waste materials which could serve for fuel production is being extensively explored. In order to increase their stability and cost-effectiveness of their usage, application of their immobilized forms are preferred over free enzymes. Hereby, we tested eight different LifetechTM immobilization supports differing in polarity, porosity and functional groups as carriers for Asspergillus Niger cellulase immobilization. Most promising carrier was methacrylate based, with primary amino groups, C6 "space arm" and pores with diameter of 60-120 nm - LifetechTM ECR8409F. For this support, most important immobilization parameters were investigated and after 3 h at pH 6 with initial protein concentration of 23.3 mg/g support immobilized cellulase with 406 IU/g (with carboxymethyl cellulose as a substrate) was obtained. This preparation was successfully applied in the hydrolysis of lignocellulosic fraction of sunflower seed meal, which is widely available byproduct of sunflower seed meal fractionation for protein-rich fractions production. Initial reaction rates and yields of reducing sugars were unchanged comparing to free enzyme, indicating that there were no significant diffusion limitations for substrate to approach active sites of A. Niger cellulase molecules immobilized onto LifetechTM ECR8409F support., Celulaze su enzimi koji katalizuju hidrolizu celuloze i u širokoj su primeni u brojnim granama industrije. U poslednje vreme, mogućnost njihove primene u tretiranju različitih opadnih agroindustrijskih sirovina u cilju dobijanja biogoriva intenzivno se istražuje. Da bi se povećale stabilnost i ekonomičnost njihove primene, potrebno je umesto slobodnog enzima koristiti imobilisane forme. U ovom istraživanju osam LifetechTM nosača različitih polarnosti, poroznosti i funkcionalnih grupa. testirano je za imobilizaciju celulaze producenta Aspergillus Niger. Najpogodniji nosač bio je na bazi metakrilata, sa primarnim amino grupama, C6 "dugom nožicom" i porama prečnika 60-120 nm - LifetechTM ECR8409F. Za ovaj nosač određeni su najznačajniji uslovi imobilizacije i nakon 3 sata na pH 6, pri početnoj koncentraciji proteina od 23,3 mg/g nosača dobijena je imobilisana celulaza aktivnosti 406 IU/g (sa karboksi metil celulozom kao supstratom). Ovaj preparat uspešno je primenjen u reakciji hidrolize lignocelulozne frakcije suncokretove sačme, koja prestavlja nusproizvod frakcionisanja suncokretove sačme pri dobijanju frakcija bogatih proteinima. Početne brzine reakcije i prinosi redukujućih šećera bili su nepromenjeni u odnosu na slobodan enzim, ukazujući da nije bilo značajnog uticaja difuzionih limitacija pri prilasku supstrata aktivnim centrima molekula A. Niger celulaze imobilisane na LifetechTM ECR8409F nosač.",
publisher = "Institute for Food Technology, Novi Sad",
journal = "Food and Feed Research",
title = "Immobilization of Aspergillus Niger cellulase onto Lifetech TM carriers and its application in the hydrolysis of sunflower seed meal lignocellulosic fraction, Imobilizacija Aspergillus Niger celulaze na Lifetech TM nosače i primena u reakciji hidrolize lignocelulozne frakcije suncokretove sačme",
pages = "169-161",
number = "2",
volume = "46",
doi = "10.5937/FFR1902161C"
}

Ćorović, M., Simović, M., Milivojević, A., Banjanac, K., Katić, K. D.,& Bezbradica, D.. (2019). Immobilization of Aspergillus Niger cellulase onto Lifetech TM carriers and its application in the hydrolysis of sunflower seed meal lignocellulosic fraction. in Food and Feed Research
Institute for Food Technology, Novi Sad., 46(2), 161-169.
https://doi.org/10.5937/FFR1902161C

Ćorović M, Simović M, Milivojević A, Banjanac K, Katić KD, Bezbradica D. Immobilization of Aspergillus Niger cellulase onto Lifetech TM carriers and its application in the hydrolysis of sunflower seed meal lignocellulosic fraction. in Food and Feed Research. 2019;46(2):161-169.
doi:10.5937/FFR1902161C .

Ćorović, Marija, Simović, Milica, Milivojević, Ana, Banjanac, Katarina, Katić, Katarina D., Bezbradica, Dejan, "Immobilization of Aspergillus Niger cellulase onto Lifetech TM carriers and its application in the hydrolysis of sunflower seed meal lignocellulosic fraction" in Food and Feed Research, 46, no. 2 (2019):161-169,
https://doi.org/10.5937/FFR1902161C . .

TY  - THES
AU  - Milivojević, Ana
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7678
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:22863/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=24141321
UR  - https://nardus.mpn.gov.rs/handle/123456789/17584
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4681
AB  - Within this doctoral dissertation, the possibility of efficient and economical enzymatic synthesis of esters of three flavonoids (floridzin, naringin and esculin) using different acyl donors and immobilized lipases B from C. antarctica as a biocatalyst was investigated. Additionally, transdermal diffusion characteristics of synthesized esters from various cosmetic formulations were determined. Synthesis of esters of the three aforementioned flavonoids using oleic acid as an acyl donor and Novozym® 435 as a biocatalyst was optimized and it was shown that the concentration of flavonoid and substrate molar ratio were two the most important factors influencing the synthesis process. The maximum conversion degree of all three flavonoids was achieved at a minimum initial flavonoid concentration, 10 mM. On the other hand, the optimum conditions for product yield as output depend on the acyl acceptor used, given that 30 mM and 70 mM were optimal concentrations of naringin and esculin for achieving the highest product yields. When it comes to the substrate molar ratio, it was shown that the large excess of oleic acid (1:18 for the conversion degree and 1:13 for the specific yield) was preferable for synthesis of floridzin-oleate, while for the esterification of other two flavonoids, five fold excess of oleic acid was optimal for high product yields. When it comes to the effect of fatty acids chain length (C2-C18) and unsaturation degree on floridzin-oleate synthesis, it was shown that high conversion degrees, over 70 % were obtained in reactions with all examined fatty acids. In order to develop the more economical process of flavonoid esterification, in the second phase od this thesis, alternative, cheaper acyl donors, such as triacetin and acetic anhydride for the synthesis of flavonoid acetate, and various vegetable oils (coconut, linseed and sunflower oil) for the synthesis of long chain fatty acid esters were examined. Triacetin proved to be the appropriate substrate for the synthesis of the acetyl derivative of flavonoids and eco-friendly, solvent-free enzymatic acetylation of floridzin was optimized. The mixture of floridzin-6 "-O-acetate and floridzin-3", 6 "-O-diacetate was obtained as a product of this reaction. Kinetic study revealed that acetylation process could be regarded as a two-step mono-substrate reaction and, within entire reaction time, both stages were adequately described by reversible Michaelis-Menten kinetics. When it comes to the usage of natural vegetable oils as an alternative acyl donors, it was shown that all examined oils were good substrates for esterification of all three flavonoids (conversion degree above 65%), whereby the mixtures of different fatty acid esters of flavonoids were obtained as products of reactions. By using experimental design and response surface methodology (RSM), the esterification of esculin using linseed oil as acyl donor was optimized and under optimal conditions high molar conversion (78.5%), product yield (19.13 mg/ml), as well as specific yield (2.83 mmol/genzyme) were achieved. Determined optimums were successfully applied in esculin transesterification with other oils. As an another way to contribute to the development of a more cost-effective esterification process, CAL B immobilization on the three selected LifeTechTM carriers with epoxy (ECR 8285F), octadecyl (ECR 8806M) and amino (ECR 8409F) functional groups was optimized. Prepared immobilized enzymes were applied as biocatalyst for esterification of the three aforementioned flavonoids, and the lipase immobilized on the carriers with hydrophobic and epoxy group have shown very good esterification activity and excellent operational stability during the 15 reaction cycles. In order to extend the use of flavonoid esters, the possibility of their incorporation into various cosmetic formulations was examined and transdermal diffusion of synthesized esters using Strat-MTM membrane was investigated. The presence of lag phase was observed in all experiments and it was shown that the effective diffusion coefficient of esters decreased with an increase in the number of C atoms and with decrease in the number of double bonds in the fatty acid chain. Emulsions in which the water was continuous phase proved to be the most suitable systems for the incorporation of flavonoid esters, and the highest effective diffusion coefficients were achieved by release of esters from gel emulsions. Finally, in order to investigate the possibility of commercial application of flavonoid esters obtained in reaction with natural oils, the stability of the prepared gel emulsions, their hydration effect and irritability after topical administration was assessed. The prepared gel emulsions have shown good stability even after 2 months of storage. Moreover, all the examined formulations have shown excellent hydration effect, nonirritant character and overall dermatological compatibility.
AB  - U okviru ove doktorske disertacije ispitana je mogućnost efikasne i ekonomiĉne enzimske sinteze estara tri flavonoida (floridzina, naringina i eskulina) korišćenjem razliĉitih acil-donora i imobilisane lipaze tipa B iz C. antarctica kao biokatalizatora. Pored toga, ispitan je transdermalni prenos i odreĊene su difuzione karakteristike sintetisanih estara iz razliĉitih kozmetiĉkih formulacija. Sinteza estara tri pomenuta flavonoida korišćenjem oleinske kiseline kao acil-donora i Novozym® 435 kao biokatalizatora je optimizovana i pokazano je da su koncentracija flavonoida i molski odnos supstrata dva najbitnija faktora koja utiĉu na tok sinteze. Maksimalni stepen konverzije sva tri flavonoida postiže se pri najmanjoj poĉetnoj koncentraciji flavonoida, 10 mM. Sa druge strane, uoĉeno je da za postizanje maksimalnog prinosa proizvoda optimalni uslovi zavise od primenjenog acil-akceptora s obzirom da 30 mM naringina i 70 mM eskulina predstavljaju optimalne poĉetne koncentracije ova dva flavonoida za dobijanje visokog prinosa proizvoda. Kada je reĉ o molskom odnosu supstrata, pokazano je da reakciji sinteze floridzin-oleata pogoduje veliki višak oleinske kiseline (1:18 za stepen konverzije i 1:13 za specifiĉni prinos), dok je za esterifikaciju druga dva flavonoida optimalna pet puta veća koncentracija acil-donora u reakcionoj smeši. Što se tiĉe uticaja dužine lanca (C2-C18) i stepena nezasićenosti masne kiseline na sintezu floridzin-oleata, primenom svih kiselina ostvareni su visoki stepeni konverzije, preko 70 %. U cilju razvoja isplativijeg procesa esterifikacije flavonoida, u drugoj fazi ove disertacije, ispitani su i alternativni, jeftiniji acil-donori, kao što su triacetin i anhidrid sirćetne kiseline za sintezu flavonoid-acetata, i razliĉita biljna ulja (kokosovo, laneno i suncokretovo ulje) za sintezu estara viših masnih kiselina. Triacetin se pokazao kao odgovarajući supstrat za sintezu acetil-derivata flavonoida, pri ĉemu je postupak acetilovanja floridzina optimizovan u ekološki prihvatljivom sistemu bez rastvaraĉa. Kao proizvod reakcije acetilovanja floridzina dobijena je smeša estara floridzin-6"-O-acetata i floridzin-3",6"-O-diacetata. Kinetiĉka studija je pokazala da se proces acetilovanja flavonoida može opisati dvostepenom monosupstratnom reakcijom, a da su oba stupnja reakcije adekvatno opisana reverzibilnom Mihaelis-Mentenovom kinetikom u celom ispitanom reakcionom toku. Kada je reĉ o upotrebi prirodnih biljnih ulja kao acil-donora, sva ispitana ulja su se pokazala kao dobri supstrati u reakciji esterifikacije sva tri flavonoida (ostvareni stepeni konverzije preko 65 %) pri ĉemu kao proizvodi reakcije nastaju smeše estara flavonoida i kiselina prisutnih u ulju. Primenom statistiĉkih metoda planiranja i metode odzivnih površina reakcija esterifikacije eskulina primenom lanenog ulja je optimizovana i visok stepen konverzije (78,5 %), prinos (19,13 mg/ml), kao i specifiĉni prinos proizvoda (2,83 mmol/genzima) su postignuti pod optimalnim uslovima. OdreĊeni optimalni uslovi su uspešno primenjeni i u reakcijama transesterifikacije eskulina korišćenjem druga dva ulja. Kao drugi naĉin da se doprinese razvoju isplativijeg procesa esterifikacije estara, u narednoj fazi optimizovana je imobilizacija CAL B na tri odabrana LifeTechTM nosaĉa sa epoksi (ECR 8285F), oktadecil (ECR 8806M) i amino (ECR 8409F) funkcionalnim grupama. Pripremljeni imobilisani preparati primenjeni su u reakciji sinteze estara tri pomenuta flavonoida, pri ĉemu lipaze imobilisane na hidrofobni i nosaĉ sa epoksi grupama pokazuju veoma dobru esterifikacionu aktivnost i odliĉnu operativnu stabilnost i tokom 15 ciklusa reakcije esterifikacije. U cilju proširenja primene estara flavonoida, ispitana je mogućnost njihovog inkorporiranja u razliĉite kozmetiĉke formulacije i praćena je kinetika otpuštanja sintetisanih estara kroz Strat-MTM membranu koja služi kao model sistem za transdermalni prenos. U svim eksperimentima primećeno je da do prenosa komponente dolazi sa zakašnjenjem (lag faza) i da koeficijent difuzije estara opada sa povećanjem broja C atoma i smanjenjem broja dvostrukih veza u lancu masne kiseline. Formulacije u kojima je voda kontinualna faza su se pokazale kao najpogodniji sistemi za inkorporiranje estara, a najveći efektivni koeficijenti difuzije ostvareni su prilikom otpuštanja estara iz gel-emulzija. I na kraju, u cilju ispitivanja mogućnosti komercijalne primene estara flavonoida dobijenih u reakciji sa prirodnim uljima procenjena je stabilnost pripremljenih gel-emulzija, odreĊen efekat hidratacije i stepen iritabilnosti nakon topikalne primene. Pripremljene gel-emulzije pokazuju dobru stabilnost i nakon 2 meseca skladištenja. Štaviše, sve ispitane formulacije pokazuju dobar efekat hidratacije kože, uz minimalnu iritabilnost pojedinih formulacija i odliĉnu dermatološku kompatibilnost.
PB  - Univerzitet u Beogradu, Tehnološko-metalurški fakultet
T1  - Enzymatic syntesis of flavonoid esters and controlled release from cosmetic formulation
T1  - Enzimska sinteza estara flavonoida i kontrolisano otpuštanje iz kozmetičkih formulacija
UR  - https://hdl.handle.net/21.15107/rcub_nardus_17584
ER  - 

@phdthesis{
author = "Milivojević, Ana",
year = "2019",
abstract = "Within this doctoral dissertation, the possibility of efficient and economical enzymatic synthesis of esters of three flavonoids (floridzin, naringin and esculin) using different acyl donors and immobilized lipases B from C. antarctica as a biocatalyst was investigated. Additionally, transdermal diffusion characteristics of synthesized esters from various cosmetic formulations were determined. Synthesis of esters of the three aforementioned flavonoids using oleic acid as an acyl donor and Novozym® 435 as a biocatalyst was optimized and it was shown that the concentration of flavonoid and substrate molar ratio were two the most important factors influencing the synthesis process. The maximum conversion degree of all three flavonoids was achieved at a minimum initial flavonoid concentration, 10 mM. On the other hand, the optimum conditions for product yield as output depend on the acyl acceptor used, given that 30 mM and 70 mM were optimal concentrations of naringin and esculin for achieving the highest product yields. When it comes to the substrate molar ratio, it was shown that the large excess of oleic acid (1:18 for the conversion degree and 1:13 for the specific yield) was preferable for synthesis of floridzin-oleate, while for the esterification of other two flavonoids, five fold excess of oleic acid was optimal for high product yields. When it comes to the effect of fatty acids chain length (C2-C18) and unsaturation degree on floridzin-oleate synthesis, it was shown that high conversion degrees, over 70 % were obtained in reactions with all examined fatty acids. In order to develop the more economical process of flavonoid esterification, in the second phase od this thesis, alternative, cheaper acyl donors, such as triacetin and acetic anhydride for the synthesis of flavonoid acetate, and various vegetable oils (coconut, linseed and sunflower oil) for the synthesis of long chain fatty acid esters were examined. Triacetin proved to be the appropriate substrate for the synthesis of the acetyl derivative of flavonoids and eco-friendly, solvent-free enzymatic acetylation of floridzin was optimized. The mixture of floridzin-6 "-O-acetate and floridzin-3", 6 "-O-diacetate was obtained as a product of this reaction. Kinetic study revealed that acetylation process could be regarded as a two-step mono-substrate reaction and, within entire reaction time, both stages were adequately described by reversible Michaelis-Menten kinetics. When it comes to the usage of natural vegetable oils as an alternative acyl donors, it was shown that all examined oils were good substrates for esterification of all three flavonoids (conversion degree above 65%), whereby the mixtures of different fatty acid esters of flavonoids were obtained as products of reactions. By using experimental design and response surface methodology (RSM), the esterification of esculin using linseed oil as acyl donor was optimized and under optimal conditions high molar conversion (78.5%), product yield (19.13 mg/ml), as well as specific yield (2.83 mmol/genzyme) were achieved. Determined optimums were successfully applied in esculin transesterification with other oils. As an another way to contribute to the development of a more cost-effective esterification process, CAL B immobilization on the three selected LifeTechTM carriers with epoxy (ECR 8285F), octadecyl (ECR 8806M) and amino (ECR 8409F) functional groups was optimized. Prepared immobilized enzymes were applied as biocatalyst for esterification of the three aforementioned flavonoids, and the lipase immobilized on the carriers with hydrophobic and epoxy group have shown very good esterification activity and excellent operational stability during the 15 reaction cycles. In order to extend the use of flavonoid esters, the possibility of their incorporation into various cosmetic formulations was examined and transdermal diffusion of synthesized esters using Strat-MTM membrane was investigated. The presence of lag phase was observed in all experiments and it was shown that the effective diffusion coefficient of esters decreased with an increase in the number of C atoms and with decrease in the number of double bonds in the fatty acid chain. Emulsions in which the water was continuous phase proved to be the most suitable systems for the incorporation of flavonoid esters, and the highest effective diffusion coefficients were achieved by release of esters from gel emulsions. Finally, in order to investigate the possibility of commercial application of flavonoid esters obtained in reaction with natural oils, the stability of the prepared gel emulsions, their hydration effect and irritability after topical administration was assessed. The prepared gel emulsions have shown good stability even after 2 months of storage. Moreover, all the examined formulations have shown excellent hydration effect, nonirritant character and overall dermatological compatibility., U okviru ove doktorske disertacije ispitana je mogućnost efikasne i ekonomiĉne enzimske sinteze estara tri flavonoida (floridzina, naringina i eskulina) korišćenjem razliĉitih acil-donora i imobilisane lipaze tipa B iz C. antarctica kao biokatalizatora. Pored toga, ispitan je transdermalni prenos i odreĊene su difuzione karakteristike sintetisanih estara iz razliĉitih kozmetiĉkih formulacija. Sinteza estara tri pomenuta flavonoida korišćenjem oleinske kiseline kao acil-donora i Novozym® 435 kao biokatalizatora je optimizovana i pokazano je da su koncentracija flavonoida i molski odnos supstrata dva najbitnija faktora koja utiĉu na tok sinteze. Maksimalni stepen konverzije sva tri flavonoida postiže se pri najmanjoj poĉetnoj koncentraciji flavonoida, 10 mM. Sa druge strane, uoĉeno je da za postizanje maksimalnog prinosa proizvoda optimalni uslovi zavise od primenjenog acil-akceptora s obzirom da 30 mM naringina i 70 mM eskulina predstavljaju optimalne poĉetne koncentracije ova dva flavonoida za dobijanje visokog prinosa proizvoda. Kada je reĉ o molskom odnosu supstrata, pokazano je da reakciji sinteze floridzin-oleata pogoduje veliki višak oleinske kiseline (1:18 za stepen konverzije i 1:13 za specifiĉni prinos), dok je za esterifikaciju druga dva flavonoida optimalna pet puta veća koncentracija acil-donora u reakcionoj smeši. Što se tiĉe uticaja dužine lanca (C2-C18) i stepena nezasićenosti masne kiseline na sintezu floridzin-oleata, primenom svih kiselina ostvareni su visoki stepeni konverzije, preko 70 %. U cilju razvoja isplativijeg procesa esterifikacije flavonoida, u drugoj fazi ove disertacije, ispitani su i alternativni, jeftiniji acil-donori, kao što su triacetin i anhidrid sirćetne kiseline za sintezu flavonoid-acetata, i razliĉita biljna ulja (kokosovo, laneno i suncokretovo ulje) za sintezu estara viših masnih kiselina. Triacetin se pokazao kao odgovarajući supstrat za sintezu acetil-derivata flavonoida, pri ĉemu je postupak acetilovanja floridzina optimizovan u ekološki prihvatljivom sistemu bez rastvaraĉa. Kao proizvod reakcije acetilovanja floridzina dobijena je smeša estara floridzin-6"-O-acetata i floridzin-3",6"-O-diacetata. Kinetiĉka studija je pokazala da se proces acetilovanja flavonoida može opisati dvostepenom monosupstratnom reakcijom, a da su oba stupnja reakcije adekvatno opisana reverzibilnom Mihaelis-Mentenovom kinetikom u celom ispitanom reakcionom toku. Kada je reĉ o upotrebi prirodnih biljnih ulja kao acil-donora, sva ispitana ulja su se pokazala kao dobri supstrati u reakciji esterifikacije sva tri flavonoida (ostvareni stepeni konverzije preko 65 %) pri ĉemu kao proizvodi reakcije nastaju smeše estara flavonoida i kiselina prisutnih u ulju. Primenom statistiĉkih metoda planiranja i metode odzivnih površina reakcija esterifikacije eskulina primenom lanenog ulja je optimizovana i visok stepen konverzije (78,5 %), prinos (19,13 mg/ml), kao i specifiĉni prinos proizvoda (2,83 mmol/genzima) su postignuti pod optimalnim uslovima. OdreĊeni optimalni uslovi su uspešno primenjeni i u reakcijama transesterifikacije eskulina korišćenjem druga dva ulja. Kao drugi naĉin da se doprinese razvoju isplativijeg procesa esterifikacije estara, u narednoj fazi optimizovana je imobilizacija CAL B na tri odabrana LifeTechTM nosaĉa sa epoksi (ECR 8285F), oktadecil (ECR 8806M) i amino (ECR 8409F) funkcionalnim grupama. Pripremljeni imobilisani preparati primenjeni su u reakciji sinteze estara tri pomenuta flavonoida, pri ĉemu lipaze imobilisane na hidrofobni i nosaĉ sa epoksi grupama pokazuju veoma dobru esterifikacionu aktivnost i odliĉnu operativnu stabilnost i tokom 15 ciklusa reakcije esterifikacije. U cilju proširenja primene estara flavonoida, ispitana je mogućnost njihovog inkorporiranja u razliĉite kozmetiĉke formulacije i praćena je kinetika otpuštanja sintetisanih estara kroz Strat-MTM membranu koja služi kao model sistem za transdermalni prenos. U svim eksperimentima primećeno je da do prenosa komponente dolazi sa zakašnjenjem (lag faza) i da koeficijent difuzije estara opada sa povećanjem broja C atoma i smanjenjem broja dvostrukih veza u lancu masne kiseline. Formulacije u kojima je voda kontinualna faza su se pokazale kao najpogodniji sistemi za inkorporiranje estara, a najveći efektivni koeficijenti difuzije ostvareni su prilikom otpuštanja estara iz gel-emulzija. I na kraju, u cilju ispitivanja mogućnosti komercijalne primene estara flavonoida dobijenih u reakciji sa prirodnim uljima procenjena je stabilnost pripremljenih gel-emulzija, odreĊen efekat hidratacije i stepen iritabilnosti nakon topikalne primene. Pripremljene gel-emulzije pokazuju dobru stabilnost i nakon 2 meseca skladištenja. Štaviše, sve ispitane formulacije pokazuju dobar efekat hidratacije kože, uz minimalnu iritabilnost pojedinih formulacija i odliĉnu dermatološku kompatibilnost.",
publisher = "Univerzitet u Beogradu, Tehnološko-metalurški fakultet",
title = "Enzymatic syntesis of flavonoid esters and controlled release from cosmetic formulation, Enzimska sinteza estara flavonoida i kontrolisano otpuštanje iz kozmetičkih formulacija",
url = "https://hdl.handle.net/21.15107/rcub_nardus_17584"
}

Milivojević, A.. (2019). Enzymatic syntesis of flavonoid esters and controlled release from cosmetic formulation. 
Univerzitet u Beogradu, Tehnološko-metalurški fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_17584

Milivojević A. Enzymatic syntesis of flavonoid esters and controlled release from cosmetic formulation. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_17584 .

Milivojević, Ana, "Enzymatic syntesis of flavonoid esters and controlled release from cosmetic formulation" (2019),
https://hdl.handle.net/21.15107/rcub_nardus_17584 .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
AU  - Kalušević, Ana
AU  - Nedović, Viktor
AU  - Pavlović, Vladimir
AU  - Dimitrijević-Branković, Suzana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4048
AB  - Agar-based composites with different Zn-carbonate mineral phase content were prepared by in situ mineralization and the solvent casting method. The mineral phase within the composite films was identified as hydrozincite, Zn-5(CO3)(2)(OH)(6). The presence of the mineral phase improved, both mechanical and water vapor permeability properties of the obtained composite films, in a concentration-dependent manner. The release of zinc ions from composite films is in accepted levels (up to 2.5%), and sufficient to provide complete inhibition growth of S. Aureus. The results of this study suggest that agar/Zn-carbonate composites could be potentially used as affordable, eco-friendly and functional materials with tunable properties for food packaging, agriculture or biomedical application. In situ procedure offers possibilities for tailoring the physical-chemical properties of composite films, by varying the Zn-mineral phase load.
PB  - Elsevier, Amsterdam
T2  - Materials Letters
T1  - Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films
VL  - 255
DO  - 10.1016/j.matlet.2019.126572
ER  - 

@article{
author = "Radovanović, Neda and Malagurski, Ivana and Lević, Steva and Nešić, Aleksandra and Cabrera-Barjas, Gustavo and Kalušević, Ana and Nedović, Viktor and Pavlović, Vladimir and Dimitrijević-Branković, Suzana",
year = "2019",
abstract = "Agar-based composites with different Zn-carbonate mineral phase content were prepared by in situ mineralization and the solvent casting method. The mineral phase within the composite films was identified as hydrozincite, Zn-5(CO3)(2)(OH)(6). The presence of the mineral phase improved, both mechanical and water vapor permeability properties of the obtained composite films, in a concentration-dependent manner. The release of zinc ions from composite films is in accepted levels (up to 2.5%), and sufficient to provide complete inhibition growth of S. Aureus. The results of this study suggest that agar/Zn-carbonate composites could be potentially used as affordable, eco-friendly and functional materials with tunable properties for food packaging, agriculture or biomedical application. In situ procedure offers possibilities for tailoring the physical-chemical properties of composite films, by varying the Zn-mineral phase load.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Letters",
title = "Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films",
volume = "255",
doi = "10.1016/j.matlet.2019.126572"
}

Radovanović, N., Malagurski, I., Lević, S., Nešić, A., Cabrera-Barjas, G., Kalušević, A., Nedović, V., Pavlović, V.,& Dimitrijević-Branković, S.. (2019). Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films. in Materials Letters
Elsevier, Amsterdam., 255.
https://doi.org/10.1016/j.matlet.2019.126572

Radovanović N, Malagurski I, Lević S, Nešić A, Cabrera-Barjas G, Kalušević A, Nedović V, Pavlović V, Dimitrijević-Branković S. Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films. in Materials Letters. 2019;255.
doi:10.1016/j.matlet.2019.126572 .

Radovanović, Neda, Malagurski, Ivana, Lević, Steva, Nešić, Aleksandra, Cabrera-Barjas, Gustavo, Kalušević, Ana, Nedović, Viktor, Pavlović, Vladimir, Dimitrijević-Branković, Suzana, "Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films" in Materials Letters, 255 (2019),
https://doi.org/10.1016/j.matlet.2019.126572 . .

TY  - JOUR
AU  - Buntić, Aneta
AU  - Stajković-Srbinović, Olivera S.
AU  - Knežević, Magdalena M.
AU  - Rasulić, Nataša V.
AU  - Kuzmanović, Đorđe Ž.
AU  - Dimitrijević-Branković, Suzana
AU  - Delić, Dušica I.
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4291
AB  - Soil microorganisms, which are usually found in plant rhizosphere, have a wide spectrum of beneficial effects on the promotion of plant growth. The most comprehensively studied bacteria with these effects belong to the Bacillus genus. In this study, seven Bacillus isolates from Medicago sativa rhizosphere soils were isolated. Plant growth promoting characteristics of these isolates, such as production of indole-3aceticacid, siderophores and hydrogen cyanide were tested. The induction of wheat (Triticum aestivum) and barley (Hordeum jubatum) seed germination was evaluated in vitro. Isolate BMG2 produced the highest indole-3-aceticacid of 24.89 µg/mL. The length of roots of barley increased up to 60%, while the length of shoots of barley increased 2.23 times afterapplying isolatesPAZE-6 and BMG1, respectively. In addition, isolates BMG1, BMG2 and PAZE-6 improved germination of both types of seeds and showed ability to produce useful substances such as siderophores and indole-3-acetic acid. Further, these isolates could be used in the production of liquid crop additives that can improve the total yield of cultivated plants, especially barley.
AB  - Zemljišne bakterije iz rizosfere korena biljaka najčešće imaju širok spektar pozitivnog dejstva na biljke I mogu poboljšati njhiov rast. Vrste roda Bacillus jedne su od najistraživanijih bakterija koje mogu doprinositi poboljšanju rasta biljaka. U ovom radu je izolovano sedam Bacillus izolata iz rizosfere lucerke (Medicago sativa). Ispitivana je sposobnost produkcije jedinjenja kao što su vodonik-cijanid, indol-3-sirćetna kiselina I siderofore. Procenjena je I sposobnost indukcije klijavosti na semena pšenice (Triticum aestivum) I ječma (Hordeum jubatum). Izolat BMG2 je proizveo najviše indol-3-sirćetne kiseline (28.89 µg/mL). Dužina korenčića je povećana za 60%, dok je dužina izdanka uvećana 2.23 puta primenom izolata PAZE-6 i BMG1. Pored toga, izolati BMG1, BMG2 i PAZE-6, su poboljšali klijavost obe vrste semena I pokazali sposobnost da proizvedu korisne supstance kao što su sideroforei indol-3sirćetna kiselina. Na dalje bi ovi izolati mogli da se koriste u proizvodnji tečnih aditiva koji bi povećali prinos kultivisanih biljaka, a posebno ječma.
PB  - Serbian Soil Science Society, Belgrade
T2  - Zemljište i biljka
T1  - The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination
T1  - Uticaj bakterijskih izolata iz zemljišta rizosfere na indukciju klijavosti semena pšenice i ječma
EP  - 11
IS  - 2
SP  - 1
VL  - 68
DO  - 10.5937/ZemBilj1902001B
ER  - 

@article{
author = "Buntić, Aneta and Stajković-Srbinović, Olivera S. and Knežević, Magdalena M. and Rasulić, Nataša V. and Kuzmanović, Đorđe Ž. and Dimitrijević-Branković, Suzana and Delić, Dušica I.",
year = "2019",
abstract = "Soil microorganisms, which are usually found in plant rhizosphere, have a wide spectrum of beneficial effects on the promotion of plant growth. The most comprehensively studied bacteria with these effects belong to the Bacillus genus. In this study, seven Bacillus isolates from Medicago sativa rhizosphere soils were isolated. Plant growth promoting characteristics of these isolates, such as production of indole-3aceticacid, siderophores and hydrogen cyanide were tested. The induction of wheat (Triticum aestivum) and barley (Hordeum jubatum) seed germination was evaluated in vitro. Isolate BMG2 produced the highest indole-3-aceticacid of 24.89 µg/mL. The length of roots of barley increased up to 60%, while the length of shoots of barley increased 2.23 times afterapplying isolatesPAZE-6 and BMG1, respectively. In addition, isolates BMG1, BMG2 and PAZE-6 improved germination of both types of seeds and showed ability to produce useful substances such as siderophores and indole-3-acetic acid. Further, these isolates could be used in the production of liquid crop additives that can improve the total yield of cultivated plants, especially barley., Zemljišne bakterije iz rizosfere korena biljaka najčešće imaju širok spektar pozitivnog dejstva na biljke I mogu poboljšati njhiov rast. Vrste roda Bacillus jedne su od najistraživanijih bakterija koje mogu doprinositi poboljšanju rasta biljaka. U ovom radu je izolovano sedam Bacillus izolata iz rizosfere lucerke (Medicago sativa). Ispitivana je sposobnost produkcije jedinjenja kao što su vodonik-cijanid, indol-3-sirćetna kiselina I siderofore. Procenjena je I sposobnost indukcije klijavosti na semena pšenice (Triticum aestivum) I ječma (Hordeum jubatum). Izolat BMG2 je proizveo najviše indol-3-sirćetne kiseline (28.89 µg/mL). Dužina korenčića je povećana za 60%, dok je dužina izdanka uvećana 2.23 puta primenom izolata PAZE-6 i BMG1. Pored toga, izolati BMG1, BMG2 i PAZE-6, su poboljšali klijavost obe vrste semena I pokazali sposobnost da proizvedu korisne supstance kao što su sideroforei indol-3sirćetna kiselina. Na dalje bi ovi izolati mogli da se koriste u proizvodnji tečnih aditiva koji bi povećali prinos kultivisanih biljaka, a posebno ječma.",
publisher = "Serbian Soil Science Society, Belgrade",
journal = "Zemljište i biljka",
title = "The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination, Uticaj bakterijskih izolata iz zemljišta rizosfere na indukciju klijavosti semena pšenice i ječma",
pages = "11-1",
number = "2",
volume = "68",
doi = "10.5937/ZemBilj1902001B"
}

Buntić, A., Stajković-Srbinović, O. S., Knežević, M. M., Rasulić, N. V., Kuzmanović, Đ. Ž., Dimitrijević-Branković, S.,& Delić, D. I.. (2019). The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination. in Zemljište i biljka
Serbian Soil Science Society, Belgrade., 68(2), 1-11.
https://doi.org/10.5937/ZemBilj1902001B

Buntić A, Stajković-Srbinović OS, Knežević MM, Rasulić NV, Kuzmanović ĐŽ, Dimitrijević-Branković S, Delić DI. The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination. in Zemljište i biljka. 2019;68(2):1-11.
doi:10.5937/ZemBilj1902001B .

Buntić, Aneta, Stajković-Srbinović, Olivera S., Knežević, Magdalena M., Rasulić, Nataša V., Kuzmanović, Đorđe Ž., Dimitrijević-Branković, Suzana, Delić, Dušica I., "The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination" in Zemljište i biljka, 68, no. 2 (2019):1-11,
https://doi.org/10.5937/ZemBilj1902001B . .

TY  - CONF
AU  - Mihajlovski, Katarina
AU  - Milić, Marija
AU  - Dimitrijević-Branković, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6147
AB  - In recent years, there have been an incresaing interest for reuse of agricultural by-products expecially in the field of microbial enzymes production. Theese kind of a agricultural waste are abundant, cheep and represent a good source of fermentable sugars and proteins which are necessary for microorganisms growth and enzymes production. Because of the stil high price of commercial enzymes, microbial enzymes produced by this way could reduce the cost of entire process.
The potential of a novel bacterial strain Streptomyces fluvissimus CKS7, previously isolated from a soil, to produce industrially important enzymes was evaluated using selective agar plates. The strain was able to produce extracellular cellulase (CMC-ase and Avicelase), amylase, pectinase and xylanase. 
Different types of agricultural by-products (wheat bran, barley bran, ray bran, sunflower meal and soy meal) were used as substrates for CKS 7 growth and enzymes production. Solid state fermentation (SSF) was performed using these waste substrates. Among tested agricultural by-products, ray bran showed maximum enzymes production. During SSF on rye bran, the strain CKS7 produced several enzymes: cellulase (Avicelase and CMC-ase), amylase, pectinase and xylanase. An optimum solid: moisture ratio for maximum enzymes production was investigated. Maximum of CMC-ase 2.81 U/g, Avicelase 2.67 U/g, amylase 7.63 U/g, xylanase 6.84 U/g and pectinase 9.81 U/g activity was obtained at the sixt day of incubation with 10% of inoculum using rye bran as substrate. The results obtained in this study showed that agricultural by-products could be used as substrate for enzymes production.
PB  - Skopje : Society of chemists and technologists of Macedonia
C3  - Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia (with international participation) 19-22 September 2018 Ohrid, R. Macedonia, Metropol Lake Resort
T1  - Production of enzymes by a new strain Streptomyces fluvissimus CKS7 using agricultural by-products
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6147
ER  - 

@conference{
author = "Mihajlovski, Katarina and Milić, Marija and Dimitrijević-Branković, Suzana",
year = "2018",
abstract = "In recent years, there have been an incresaing interest for reuse of agricultural by-products expecially in the field of microbial enzymes production. Theese kind of a agricultural waste are abundant, cheep and represent a good source of fermentable sugars and proteins which are necessary for microorganisms growth and enzymes production. Because of the stil high price of commercial enzymes, microbial enzymes produced by this way could reduce the cost of entire process.
The potential of a novel bacterial strain Streptomyces fluvissimus CKS7, previously isolated from a soil, to produce industrially important enzymes was evaluated using selective agar plates. The strain was able to produce extracellular cellulase (CMC-ase and Avicelase), amylase, pectinase and xylanase. 
Different types of agricultural by-products (wheat bran, barley bran, ray bran, sunflower meal and soy meal) were used as substrates for CKS 7 growth and enzymes production. Solid state fermentation (SSF) was performed using these waste substrates. Among tested agricultural by-products, ray bran showed maximum enzymes production. During SSF on rye bran, the strain CKS7 produced several enzymes: cellulase (Avicelase and CMC-ase), amylase, pectinase and xylanase. An optimum solid: moisture ratio for maximum enzymes production was investigated. Maximum of CMC-ase 2.81 U/g, Avicelase 2.67 U/g, amylase 7.63 U/g, xylanase 6.84 U/g and pectinase 9.81 U/g activity was obtained at the sixt day of incubation with 10% of inoculum using rye bran as substrate. The results obtained in this study showed that agricultural by-products could be used as substrate for enzymes production.",
publisher = "Skopje : Society of chemists and technologists of Macedonia",
journal = "Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia (with international participation) 19-22 September 2018 Ohrid, R. Macedonia, Metropol Lake Resort",
title = "Production of enzymes by a new strain Streptomyces fluvissimus CKS7 using agricultural by-products",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6147"
}

Mihajlovski, K., Milić, M.,& Dimitrijević-Branković, S.. (2018). Production of enzymes by a new strain Streptomyces fluvissimus CKS7 using agricultural by-products. in Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia (with international participation) 19-22 September 2018 Ohrid, R. Macedonia, Metropol Lake Resort
Skopje : Society of chemists and technologists of Macedonia..
https://hdl.handle.net/21.15107/rcub_technorep_6147

Mihajlovski K, Milić M, Dimitrijević-Branković S. Production of enzymes by a new strain Streptomyces fluvissimus CKS7 using agricultural by-products. in Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia (with international participation) 19-22 September 2018 Ohrid, R. Macedonia, Metropol Lake Resort. 2018;.
https://hdl.handle.net/21.15107/rcub_technorep_6147 .

Mihajlovski, Katarina, Milić, Marija, Dimitrijević-Branković, Suzana, "Production of enzymes by a new strain Streptomyces fluvissimus CKS7 using agricultural by-products" in Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia (with international participation) 19-22 September 2018 Ohrid, R. Macedonia, Metropol Lake Resort (2018),
https://hdl.handle.net/21.15107/rcub_technorep_6147 .

TY  - CONF
AU  - Mihajlovski, Katarina
AU  - Milić, Marija
AU  - Marković, Darka
AU  - Dimitrijević-Branković, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6148
AB  - The microbial enzymes are biological catalysts that perform reactions in bio-processes in an economical and environmentally-friendly way as opposed to the use of chemical catalysts. Microbial enzymes, obtained by different microorganisms are of great importance for application in industrial bioprocesses [1,2]. The use of microbial enzymes in various industries (e.g., food, agriculture, chemicals, and pharmaceuticals) is increasing rapidly due to reduced processing time, low energy input, cost effectiveness, nontoxic and eco-friendly characteristics [3]. Cellulase are the largest group of industrially important enzymes with a potential to convert cellulose into fermentable sugars [4].  Amylases represents a second largest group of industrial enzymes which are used for the degradation of starch to glucose, maltose, dextrins…[5]. Xylanase are important for pentose production, fruit juice clarification…while pectinase degrade pectic substances in the cell wall of higher plants.They are important in food-processing industries, mainly for extraction and clarification of fruit juices and wines, extraction of tomato pulp, oil extraction, and tea and chocolate fermentation [1,2]. All these enzymes (cellulase, amylase, xylanase and pectinase) could be used for agro-industrial residues utilization that lead to bioethanol production. 

In this study a crude “enzymes cocktail”, that produce Streptomyces fluvissimus CKS7 was used in a hydrolysis process of different lignocellulosic waste materials. The strain CKS7 produced cellulase (CMC-ase and Avicelase), amylase, pectinase and xylanase during solid state fermentation (SSF) on agricultural by-product – rye bran. Obtained crude enzymes were used for hydrolysis of different lignocellulosic substrates: corn stalk waste, Equisetum arvense waste, Gentiana lutea waste, cotton fabric and corona treated cotton fabric. Released reducing sugars was measured during 3 days of hydrolysis at 50 °C. The results revealed that the concentration of reducing sugars was increasing during hydrolysis in all tested lignocellulosic substrates. Although the concentration of released reducing sugars are relatively low (~ 1.9 mg/ml) in all tested samples, this is the first study that deals with enzymatic potential of the S. fluvissimus.
Further experiments should be focused on optimization of enzymatic hydrolysis of lignocellulosic substrates.
PB  - Society of Chemists and Technologists of Macedonia
C3  - Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia
T1  - Possibility of using microbial enzymes produced by Streptomyces fluvissimus CKS7 in hydrolysis process
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6148
ER  - 

@conference{
author = "Mihajlovski, Katarina and Milić, Marija and Marković, Darka and Dimitrijević-Branković, Suzana",
year = "2018",
abstract = "The microbial enzymes are biological catalysts that perform reactions in bio-processes in an economical and environmentally-friendly way as opposed to the use of chemical catalysts. Microbial enzymes, obtained by different microorganisms are of great importance for application in industrial bioprocesses [1,2]. The use of microbial enzymes in various industries (e.g., food, agriculture, chemicals, and pharmaceuticals) is increasing rapidly due to reduced processing time, low energy input, cost effectiveness, nontoxic and eco-friendly characteristics [3]. Cellulase are the largest group of industrially important enzymes with a potential to convert cellulose into fermentable sugars [4].  Amylases represents a second largest group of industrial enzymes which are used for the degradation of starch to glucose, maltose, dextrins…[5]. Xylanase are important for pentose production, fruit juice clarification…while pectinase degrade pectic substances in the cell wall of higher plants.They are important in food-processing industries, mainly for extraction and clarification of fruit juices and wines, extraction of tomato pulp, oil extraction, and tea and chocolate fermentation [1,2]. All these enzymes (cellulase, amylase, xylanase and pectinase) could be used for agro-industrial residues utilization that lead to bioethanol production. 

In this study a crude “enzymes cocktail”, that produce Streptomyces fluvissimus CKS7 was used in a hydrolysis process of different lignocellulosic waste materials. The strain CKS7 produced cellulase (CMC-ase and Avicelase), amylase, pectinase and xylanase during solid state fermentation (SSF) on agricultural by-product – rye bran. Obtained crude enzymes were used for hydrolysis of different lignocellulosic substrates: corn stalk waste, Equisetum arvense waste, Gentiana lutea waste, cotton fabric and corona treated cotton fabric. Released reducing sugars was measured during 3 days of hydrolysis at 50 °C. The results revealed that the concentration of reducing sugars was increasing during hydrolysis in all tested lignocellulosic substrates. Although the concentration of released reducing sugars are relatively low (~ 1.9 mg/ml) in all tested samples, this is the first study that deals with enzymatic potential of the S. fluvissimus.
Further experiments should be focused on optimization of enzymatic hydrolysis of lignocellulosic substrates.",
publisher = "Society of Chemists and Technologists of Macedonia",
journal = "Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia",
title = "Possibility of using microbial enzymes produced by Streptomyces fluvissimus CKS7 in hydrolysis process",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6148"
}

Mihajlovski, K., Milić, M., Marković, D.,& Dimitrijević-Branković, S.. (2018). Possibility of using microbial enzymes produced by Streptomyces fluvissimus CKS7 in hydrolysis process. in Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia
Society of Chemists and Technologists of Macedonia..
https://hdl.handle.net/21.15107/rcub_technorep_6148

Mihajlovski K, Milić M, Marković D, Dimitrijević-Branković S. Possibility of using microbial enzymes produced by Streptomyces fluvissimus CKS7 in hydrolysis process. in Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia. 2018;.
https://hdl.handle.net/21.15107/rcub_technorep_6148 .

Mihajlovski, Katarina, Milić, Marija, Marković, Darka, Dimitrijević-Branković, Suzana, "Possibility of using microbial enzymes produced by Streptomyces fluvissimus CKS7 in hydrolysis process" in Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia (2018),
https://hdl.handle.net/21.15107/rcub_technorep_6148 .

TY  - CONF
AU  - Buntić, Aneta
AU  - Stajković-Srbinović, Olivera
AU  - Milić, Marija
AU  - Kuzmanović, Đorđe
AU  - Rasulić, Nataša
AU  - Dimitrijević-Branković, Suzana
AU  - Delić, Dušica
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6425
AB  - Cellulases are industrially important enzymes which can convert the most abundant natural polysaccha-ride - cellulose into glucose, making it as a renewable resource of carbohydrate for the obtaining of bio-based products and bioenergy. The latest trends in microbial cellulase production include the use of various waste products for the microorganism‘s growth, especially of agroindustrial origin, within the solid-state fermentation (SSF), as a promising technology that can potentially reduce the overall costs. Among soil bacteria, rhizobia are rarely investigated as a cellulase producer. The rhizobial soil bacte-rium Sinorhizobium meliloti strain 224 was able to grow and express the metabolic activity on the commercial substrate carboxymethyl cellulose (CMC), but also cellulose based waste materials, such as soybean stems and oat dust. The cellulase produced in a liquid medium with commercial substrates, containing 0.1% (w/v) of CMC in yeast mannitol broth and 10% of the inoculum, after 48 h of incuba-tion, at 28 °C, expressed maximum Avicelase activity of 0.077 U/mL. On the other side, the cellulase produced in a medium with waste substrates, containing 1 g of soybean waste with 10% of the inocu-lum, after 48 h of incubation, at 28 °C, expressed maximum Avicelase activity of 1.295 U/g. The hy-drolysis of Avicel indicate a pre-dominant activity of exoglucanases, those produce the glucose, with traces of other soluble sugars, showing that the crude enzyme, produced on waste material using the soil bacteria S. meliloti 224, could be used in eco-friendly processes of cellulose bioconversion and reduction of biological waste.
PB  - Soil Science Society of Kazakhstan
PB  - Federation of Eurasian Soil Science Societies
C3  - 10th International Soil Science Congress on "Environment and Soil Resources Conservation
T1  - Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates
EP  - 200
SP  - 197
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6425
ER  - 

@conference{
author = "Buntić, Aneta and Stajković-Srbinović, Olivera and Milić, Marija and Kuzmanović, Đorđe and Rasulić, Nataša and Dimitrijević-Branković, Suzana and Delić, Dušica",
year = "2018",
abstract = "Cellulases are industrially important enzymes which can convert the most abundant natural polysaccha-ride - cellulose into glucose, making it as a renewable resource of carbohydrate for the obtaining of bio-based products and bioenergy. The latest trends in microbial cellulase production include the use of various waste products for the microorganism‘s growth, especially of agroindustrial origin, within the solid-state fermentation (SSF), as a promising technology that can potentially reduce the overall costs. Among soil bacteria, rhizobia are rarely investigated as a cellulase producer. The rhizobial soil bacte-rium Sinorhizobium meliloti strain 224 was able to grow and express the metabolic activity on the commercial substrate carboxymethyl cellulose (CMC), but also cellulose based waste materials, such as soybean stems and oat dust. The cellulase produced in a liquid medium with commercial substrates, containing 0.1% (w/v) of CMC in yeast mannitol broth and 10% of the inoculum, after 48 h of incuba-tion, at 28 °C, expressed maximum Avicelase activity of 0.077 U/mL. On the other side, the cellulase produced in a medium with waste substrates, containing 1 g of soybean waste with 10% of the inocu-lum, after 48 h of incubation, at 28 °C, expressed maximum Avicelase activity of 1.295 U/g. The hy-drolysis of Avicel indicate a pre-dominant activity of exoglucanases, those produce the glucose, with traces of other soluble sugars, showing that the crude enzyme, produced on waste material using the soil bacteria S. meliloti 224, could be used in eco-friendly processes of cellulose bioconversion and reduction of biological waste.",
publisher = "Soil Science Society of Kazakhstan, Federation of Eurasian Soil Science Societies",
journal = "10th International Soil Science Congress on "Environment and Soil Resources Conservation",
title = "Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates",
pages = "200-197",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6425"
}

Buntić, A., Stajković-Srbinović, O., Milić, M., Kuzmanović, Đ., Rasulić, N., Dimitrijević-Branković, S.,& Delić, D.. (2018). Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates. in 10th International Soil Science Congress on "Environment and Soil Resources Conservation
Soil Science Society of Kazakhstan., 197-200.
https://hdl.handle.net/21.15107/rcub_technorep_6425

Buntić A, Stajković-Srbinović O, Milić M, Kuzmanović Đ, Rasulić N, Dimitrijević-Branković S, Delić D. Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates. in 10th International Soil Science Congress on "Environment and Soil Resources Conservation. 2018;:197-200.
https://hdl.handle.net/21.15107/rcub_technorep_6425 .

Buntić, Aneta, Stajković-Srbinović, Olivera, Milić, Marija, Kuzmanović, Đorđe, Rasulić, Nataša, Dimitrijević-Branković, Suzana, Delić, Dušica, "Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates" in 10th International Soil Science Congress on "Environment and Soil Resources Conservation (2018):197-200,
https://hdl.handle.net/21.15107/rcub_technorep_6425 .

TY  - CONF
AU  - Radovanović, Neda
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Malagurski, Ivana
AU  - Gordić, Milan
AU  - Nešić, Aleksandra
AU  - Dimitrijević-Branković, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6286
C3  - Polychar 26 World Forum on Advanced Materials
T1  - ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING
SP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6286
ER  - 

@conference{
author = "Radovanović, Neda and Miljković, Miona and Davidović, Slađana and Malagurski, Ivana and Gordić, Milan and Nešić, Aleksandra and Dimitrijević-Branković, Suzana",
year = "2018",
journal = "Polychar 26 World Forum on Advanced Materials",
title = "ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING",
pages = "94",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6286"
}

Radovanović, N., Miljković, M., Davidović, S., Malagurski, I., Gordić, M., Nešić, A.,& Dimitrijević-Branković, S.. (2018). ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING. in Polychar 26 World Forum on Advanced Materials, 94.
https://hdl.handle.net/21.15107/rcub_technorep_6286

Radovanović N, Miljković M, Davidović S, Malagurski I, Gordić M, Nešić A, Dimitrijević-Branković S. ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING. in Polychar 26 World Forum on Advanced Materials. 2018;:94.
https://hdl.handle.net/21.15107/rcub_technorep_6286 .

Radovanović, Neda, Miljković, Miona, Davidović, Slađana, Malagurski, Ivana, Gordić, Milan, Nešić, Aleksandra, Dimitrijević-Branković, Suzana, "ACTIVE AGAR MINERALIZED COMPOSITE FILMS INTENDED FOR FOOD PACKAGING" in Polychar 26 World Forum on Advanced Materials (2018):94,
https://hdl.handle.net/21.15107/rcub_technorep_6286 .

TY  - JOUR
AU  - Radisavljević, Anđela
AU  - Stojanović, Dušica
AU  - Perišić, Srđan
AU  - Đokić, Veljko
AU  - Radojević, Vesna
AU  - Rajilić-Stojanović, Mirjana
AU  - Uskoković, Petar
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3931
AB  - Antibiotic containing polycaprolactone (PCL) fibers were produced by using three electrospinning methods: blend, emulsion and co-axial electrospinning (labeled as S1, S2 and S3, respectively). The profiles of drug release from three different systems were studied and antimicrobial properties of produced materials were evaluated. Morphology of the produced fibers was characterized and revealed that cefazolin-loaded PCL fibers had smaller diameter compared to neat PCL fibers, while the chemical interaction between the antibiotic and PCL showed that cefazolin neither had reacted with PCL phase, nor had degraded during the electrospinning process. The crystallinity and thermal characterization of fabricated fibers showed that the addition of cefazolin decreased the crystallinity of PCL. The results of the drug release behavior of the blend and co-axial electrospun fibers was on a higher level (similar to 68% and similar to 43%, respectively) compared to the emulsion electrospun fibers (similar to 5%), after a period of 30 days. The obtained data had the best fitting with the first order model and the Higuchi model, while the Korsmeyer-Peppas model showed a Pseudo-Fickian diffusion of the drug. Antibacterial evaluations showed that cefazolin-loaded PCL fibers had better effects on Staphylococcus aureus compared to Escherichia coll. during the treatment period and that the effect of the emulsion fibers was notably weaker than the other two studied systems. The aim of the study was to test different systems for control drug release of different dynamics, which will be applied for prevent bacterial accumulation when indwelling urinary catheters, applied for different periods of time.
PB  - Elsevier, Amsterdam
T2  - European Journal of Pharmaceutical Sciences
T1  - Cefazolin-loaded polycaprolactone fibers produced via different electrospinning methods: Characterization, drug release and antibacterial effect
EP  - 36
SP  - 26
VL  - 124
DO  - 10.1016/j.ejps.2018.08.023
ER  - 

@article{
author = "Radisavljević, Anđela and Stojanović, Dušica and Perišić, Srđan and Đokić, Veljko and Radojević, Vesna and Rajilić-Stojanović, Mirjana and Uskoković, Petar",
year = "2018",
abstract = "Antibiotic containing polycaprolactone (PCL) fibers were produced by using three electrospinning methods: blend, emulsion and co-axial electrospinning (labeled as S1, S2 and S3, respectively). The profiles of drug release from three different systems were studied and antimicrobial properties of produced materials were evaluated. Morphology of the produced fibers was characterized and revealed that cefazolin-loaded PCL fibers had smaller diameter compared to neat PCL fibers, while the chemical interaction between the antibiotic and PCL showed that cefazolin neither had reacted with PCL phase, nor had degraded during the electrospinning process. The crystallinity and thermal characterization of fabricated fibers showed that the addition of cefazolin decreased the crystallinity of PCL. The results of the drug release behavior of the blend and co-axial electrospun fibers was on a higher level (similar to 68% and similar to 43%, respectively) compared to the emulsion electrospun fibers (similar to 5%), after a period of 30 days. The obtained data had the best fitting with the first order model and the Higuchi model, while the Korsmeyer-Peppas model showed a Pseudo-Fickian diffusion of the drug. Antibacterial evaluations showed that cefazolin-loaded PCL fibers had better effects on Staphylococcus aureus compared to Escherichia coll. during the treatment period and that the effect of the emulsion fibers was notably weaker than the other two studied systems. The aim of the study was to test different systems for control drug release of different dynamics, which will be applied for prevent bacterial accumulation when indwelling urinary catheters, applied for different periods of time.",
publisher = "Elsevier, Amsterdam",
journal = "European Journal of Pharmaceutical Sciences",
title = "Cefazolin-loaded polycaprolactone fibers produced via different electrospinning methods: Characterization, drug release and antibacterial effect",
pages = "36-26",
volume = "124",
doi = "10.1016/j.ejps.2018.08.023"
}

Radisavljević, A., Stojanović, D., Perišić, S., Đokić, V., Radojević, V., Rajilić-Stojanović, M.,& Uskoković, P.. (2018). Cefazolin-loaded polycaprolactone fibers produced via different electrospinning methods: Characterization, drug release and antibacterial effect. in European Journal of Pharmaceutical Sciences
Elsevier, Amsterdam., 124, 26-36.
https://doi.org/10.1016/j.ejps.2018.08.023

Radisavljević A, Stojanović D, Perišić S, Đokić V, Radojević V, Rajilić-Stojanović M, Uskoković P. Cefazolin-loaded polycaprolactone fibers produced via different electrospinning methods: Characterization, drug release and antibacterial effect. in European Journal of Pharmaceutical Sciences. 2018;124:26-36.
doi:10.1016/j.ejps.2018.08.023 .

Radisavljević, Anđela, Stojanović, Dušica, Perišić, Srđan, Đokić, Veljko, Radojević, Vesna, Rajilić-Stojanović, Mirjana, Uskoković, Petar, "Cefazolin-loaded polycaprolactone fibers produced via different electrospinning methods: Characterization, drug release and antibacterial effect" in European Journal of Pharmaceutical Sciences, 124 (2018):26-36,
https://doi.org/10.1016/j.ejps.2018.08.023 . .