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Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods
dc.creator | Salim, Abdalla Ali | |
dc.creator | Grbavčić, Sanja | |
dc.creator | Šekuljica, Nataša | |
dc.creator | Vukašinović-Sekulić, Maja | |
dc.creator | Jovanović, Jelena | |
dc.creator | Jakovetić Tanasković, Sonja | |
dc.creator | Luković, Nevena | |
dc.creator | Knežević-Jugović, Zorica | |
dc.date.accessioned | 2021-03-10T13:54:57Z | |
dc.date.available | 2021-03-10T13:54:57Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 0885-4513 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4065 | |
dc.description.abstract | Protease, cellulase, and alpha-amylase producing Bacillus subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes' recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound-assisted extraction (UAE). UAE was established to be superior method for obtaining higher yields of proteases (up to 330 IU) and alpha-amylases (825 IU), under significantly shorter extraction time and gaining more concentrated product. However, the obtained model predicts that conventional process led to a product with a higher cellulolytic activity ( gt = 7.5 IU). | en |
dc.publisher | Wiley, Hoboken | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS// | |
dc.rights | restrictedAccess | |
dc.source | Biotechnology and Applied Biochemistry | |
dc.subject | Bacillus subtilis | en |
dc.subject | protease | en |
dc.subject | cellulase | en |
dc.subject | alpha-amylase | en |
dc.subject | solid-state fermentation | en |
dc.subject | ultrasound | en |
dc.subject | extraction | en |
dc.title | Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 368 | |
dc.citation.issue | 3 | |
dc.citation.other | 66(3): 361-368 | |
dc.citation.rank | M23 | |
dc.citation.spage | 361 | |
dc.citation.volume | 66 | |
dc.identifier.doi | 10.1002/bab.1732 | |
dc.identifier.pmid | 30694578 | |
dc.identifier.scopus | 2-s2.0-85061254940 | |
dc.identifier.wos | 000476792600011 | |
dc.type.version | publishedVersion |