Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment
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2012
Authors
Jakovetić, SonjaPicazo-Espinosa, Rafael
Manzanera, Maximino
Stojanović, Željko
Prodanović, Radivoje
Miladinović, Ružica
Knežević-Jugović, Zorica
Conference object (Published version)
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Show full item recordAbstract
Immobilization of lipase B from Candida antarctica was carried out using three different
immobilization methods, and two epoxy activated supports, comercial Eupergit C and
synthesized macroporous copolymer of glycidyl methacrylate (GMA) and ethylene glycol
dimethacrylate (EGDMA). The synthesized polymer was proven to be better for CALB
immobilization at both ionic strenghts that wrere applied, 0.1M and 1.25M. Investigation of
the effect of immobilization pH on the activity of immobilized enzymes, showed that the
highest activities were obtained when immobilization process was conducted in acidic
enviorments. The highest immobilized enzyme activity, 30.5 IU/ g of dry support, was
achieved when periodate method was employed. Glutaraldehyde activation of support did
not improve activity of obtained immobilized enzymes.
Source:
2012, 129-Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
Institution/Community
Tehnološko-metalurški fakultetTY - CONF AU - Jakovetić, Sonja AU - Picazo-Espinosa, Rafael AU - Manzanera, Maximino AU - Stojanović, Željko AU - Prodanović, Radivoje AU - Miladinović, Ružica AU - Knežević-Jugović, Zorica PY - 2012 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6096 AB - Immobilization of lipase B from Candida antarctica was carried out using three different immobilization methods, and two epoxy activated supports, comercial Eupergit C and synthesized macroporous copolymer of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA). The synthesized polymer was proven to be better for CALB immobilization at both ionic strenghts that wrere applied, 0.1M and 1.25M. Investigation of the effect of immobilization pH on the activity of immobilized enzymes, showed that the highest activities were obtained when immobilization process was conducted in acidic enviorments. The highest immobilized enzyme activity, 30.5 IU/ g of dry support, was achieved when periodate method was employed. Glutaraldehyde activation of support did not improve activity of obtained immobilized enzymes. T1 - Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment SP - 129 UR - https://hdl.handle.net/21.15107/rcub_technorep_6096 ER -
@conference{ author = "Jakovetić, Sonja and Picazo-Espinosa, Rafael and Manzanera, Maximino and Stojanović, Željko and Prodanović, Radivoje and Miladinović, Ružica and Knežević-Jugović, Zorica", year = "2012", abstract = "Immobilization of lipase B from Candida antarctica was carried out using three different immobilization methods, and two epoxy activated supports, comercial Eupergit C and synthesized macroporous copolymer of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA). The synthesized polymer was proven to be better for CALB immobilization at both ionic strenghts that wrere applied, 0.1M and 1.25M. Investigation of the effect of immobilization pH on the activity of immobilized enzymes, showed that the highest activities were obtained when immobilization process was conducted in acidic enviorments. The highest immobilized enzyme activity, 30.5 IU/ g of dry support, was achieved when periodate method was employed. Glutaraldehyde activation of support did not improve activity of obtained immobilized enzymes.", title = "Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment", pages = "129", url = "https://hdl.handle.net/21.15107/rcub_technorep_6096" }
Jakovetić, S., Picazo-Espinosa, R., Manzanera, M., Stojanović, Ž., Prodanović, R., Miladinović, R.,& Knežević-Jugović, Z.. (2012). Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment. , 129. https://hdl.handle.net/21.15107/rcub_technorep_6096
Jakovetić S, Picazo-Espinosa R, Manzanera M, Stojanović Ž, Prodanović R, Miladinović R, Knežević-Jugović Z. Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment. 2012;:129. https://hdl.handle.net/21.15107/rcub_technorep_6096 .
Jakovetić, Sonja, Picazo-Espinosa, Rafael, Manzanera, Maximino, Stojanović, Željko, Prodanović, Radivoje, Miladinović, Ružica, Knežević-Jugović, Zorica, "Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment" (2012):129, https://hdl.handle.net/21.15107/rcub_technorep_6096 .