Characteristics of octadecylamine-stabilized liposomes containing soy protein hydrolysates

Abstract

Liposomes have been researched as carriers for encapsulation of bioactive protein hydrolysate, despite low stability which makes them difficult to use. Using cholesterol or octadecylamine as lipid membrane stabilizers, soy protein hydrolysate is effectively encapsulated in liposomes created with phospholipon. Presence of both stabilizers concurrently resulted the highest efficiency 49.7%, while increasing the phospholipon mass had no effect. Particle size was raised (265 to 335 nm) as a result of the octadecylamine addition, and zeta potential was positive (36.7 mV), as compared to the cholesterol addition (-25.1 mV). Liposomes enabled extended hydrolysate release with retained its bioactivity.

Lipozomi su dugo proučavani nosači za inkapsulaciju bioaktivnih proteinskih hidrolizata, uprkos niskoj stabilnost koja predstavlja problem za širu primenu. Koristeći stabilizatore holesterol i oktadecilamin, proteinski hidrolizat soje je uspešno inkapsuliran u lipozome sačinjene od fosfolipona. Prisustvo oba stabilizatora istovremeno rezultiralo je najvećom efikasnišću 49,7 %, dok povećanje početne mase fosfolipona nije imalo uticaja na efikasnot. Veličina lipozoma je povećana (sa 265 na 335 nm) kao rezultata dodavanja oktadecilamina, ali je zeta potencilaj pozitivan (36,7 mV) u poređenju sa dodatkom holesterola (-25,1 mV). Pripremljeni lipozomi omogućili su produženo oslobađanje hidrolizata soje uz očuvanje njegove bioaktivnosti.