Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves
Authors
Mijalković, JelenaŠekuljica, Nataša
Biçer, Furkan
Pavlović, Neda
Jakovetić Tanasković, Sonja
Knežević-Jugović, Zorica
Conference object (Published version)
Metadata
Show full item recordAbstract
Proteins are an essential component of the human diet and effectively are provided from
both animal and plant sources. Sourcing ribulose-1,5-bisphosphate carboxylase/oxygenase
(RuBisCO) from green biomass such as pumpkin leaves, a by-product of the oil industry,
is a promising tool for obtaining protein with an excellent nutritional profile under the
green biorafinery concept, that can be used as a substitute for animal proteins. This study
provides the results regarding the implementation of ultrasound-assisted processing (UAP)
in the protein extraction from pumpkin leaves. UAP for recovery of RuBisCO proteins was
optimized by varing the solvent-to-sample ratio, solvent pH, ultrasound amplitudes and
time. UAP appears to have a strong potential for recovering RuBisCO protein from
pumpkin leaves, as it was highly influenced by the solvent-to-sample ratio along with UAP
amplitude and time.
Proteini životinjskog i biljnog porekla čine osnovne namirnice ljudske ishrane. Prema
konceptu zelene biorafinerije, izolovanje RuBisCO proteina (ribuloza-1,5-bisfosfat
karboksilaza/oksigenaze) iz zelene biomase kakva je lišće bundeve, nusproizvod industrije
prerade uljarica, je obećavajući pristup za dobijanje proteina sa visokom nutritivnom
vrednošću koje je moguće koristiti kao zamenu za proteine životinjskog porekla. U ovom
radu, ultrazvučno potpomognutim procesom ekstrakcije izolovani su proteini iz lišća
bundeve. Ultrazvučni proces ekstrakcije RuBisCO proteina optimizovan je variranjem
odnosa rastvarač-uzorak, pH rastvarača, vremena trajanja i amplitude ultrazvuka.
Ultrazvuk je ispoljio veliki pozitivan efekat na izolovanje RuBisCO proteina iz lišća
bundeve, na šta je u najvećoj meri tokom optimizacije procesa uticao odnos rastvaračuzorak, kao i amplituda i vreme ultrazvuka.
Keywords:
Ultrasound-assisted extraction / Plant-based protein / RuBisCO / Pumpkin leaves / Extraction optimizationSource:
Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade, 2022, 174-174Publisher:
- Beograd : Srpsko hemijsko društvo = Serbian Chemical Society
Funding / projects:
- MultiPromis - Multifunctional leaf protein and assembled nanocarrier structures delivered by enzyme technology (RS-ScienceFundRS-Ideje-7751519)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200287 (Innovation Center of the Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200287)
Collections
Institution/Community
Tehnološko-metalurški fakultetTY - CONF AU - Mijalković, Jelena AU - Šekuljica, Nataša AU - Biçer, Furkan AU - Pavlović, Neda AU - Jakovetić Tanasković, Sonja AU - Knežević-Jugović, Zorica PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6252 AB - Proteins are an essential component of the human diet and effectively are provided from both animal and plant sources. Sourcing ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from green biomass such as pumpkin leaves, a by-product of the oil industry, is a promising tool for obtaining protein with an excellent nutritional profile under the green biorafinery concept, that can be used as a substitute for animal proteins. This study provides the results regarding the implementation of ultrasound-assisted processing (UAP) in the protein extraction from pumpkin leaves. UAP for recovery of RuBisCO proteins was optimized by varing the solvent-to-sample ratio, solvent pH, ultrasound amplitudes and time. UAP appears to have a strong potential for recovering RuBisCO protein from pumpkin leaves, as it was highly influenced by the solvent-to-sample ratio along with UAP amplitude and time. AB - Proteini životinjskog i biljnog porekla čine osnovne namirnice ljudske ishrane. Prema konceptu zelene biorafinerije, izolovanje RuBisCO proteina (ribuloza-1,5-bisfosfat karboksilaza/oksigenaze) iz zelene biomase kakva je lišće bundeve, nusproizvod industrije prerade uljarica, je obećavajući pristup za dobijanje proteina sa visokom nutritivnom vrednošću koje je moguće koristiti kao zamenu za proteine životinjskog porekla. U ovom radu, ultrazvučno potpomognutim procesom ekstrakcije izolovani su proteini iz lišća bundeve. Ultrazvučni proces ekstrakcije RuBisCO proteina optimizovan je variranjem odnosa rastvarač-uzorak, pH rastvarača, vremena trajanja i amplitude ultrazvuka. Ultrazvuk je ispoljio veliki pozitivan efekat na izolovanje RuBisCO proteina iz lišća bundeve, na šta je u najvećoj meri tokom optimizacije procesa uticao odnos rastvaračuzorak, kao i amplituda i vreme ultrazvuka. PB - Beograd : Srpsko hemijsko društvo = Serbian Chemical Society C3 - Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade T1 - Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves EP - 174 SP - 174 UR - https://hdl.handle.net/21.15107/rcub_technorep_6252 ER -
@conference{ author = "Mijalković, Jelena and Šekuljica, Nataša and Biçer, Furkan and Pavlović, Neda and Jakovetić Tanasković, Sonja and Knežević-Jugović, Zorica", year = "2022", abstract = "Proteins are an essential component of the human diet and effectively are provided from both animal and plant sources. Sourcing ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from green biomass such as pumpkin leaves, a by-product of the oil industry, is a promising tool for obtaining protein with an excellent nutritional profile under the green biorafinery concept, that can be used as a substitute for animal proteins. This study provides the results regarding the implementation of ultrasound-assisted processing (UAP) in the protein extraction from pumpkin leaves. UAP for recovery of RuBisCO proteins was optimized by varing the solvent-to-sample ratio, solvent pH, ultrasound amplitudes and time. UAP appears to have a strong potential for recovering RuBisCO protein from pumpkin leaves, as it was highly influenced by the solvent-to-sample ratio along with UAP amplitude and time., Proteini životinjskog i biljnog porekla čine osnovne namirnice ljudske ishrane. Prema konceptu zelene biorafinerije, izolovanje RuBisCO proteina (ribuloza-1,5-bisfosfat karboksilaza/oksigenaze) iz zelene biomase kakva je lišće bundeve, nusproizvod industrije prerade uljarica, je obećavajući pristup za dobijanje proteina sa visokom nutritivnom vrednošću koje je moguće koristiti kao zamenu za proteine životinjskog porekla. U ovom radu, ultrazvučno potpomognutim procesom ekstrakcije izolovani su proteini iz lišća bundeve. Ultrazvučni proces ekstrakcije RuBisCO proteina optimizovan je variranjem odnosa rastvarač-uzorak, pH rastvarača, vremena trajanja i amplitude ultrazvuka. Ultrazvuk je ispoljio veliki pozitivan efekat na izolovanje RuBisCO proteina iz lišća bundeve, na šta je u najvećoj meri tokom optimizacije procesa uticao odnos rastvaračuzorak, kao i amplituda i vreme ultrazvuka.", publisher = "Beograd : Srpsko hemijsko društvo = Serbian Chemical Society", journal = "Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade", title = "Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves", pages = "174-174", url = "https://hdl.handle.net/21.15107/rcub_technorep_6252" }
Mijalković, J., Šekuljica, N., Biçer, F., Pavlović, N., Jakovetić Tanasković, S.,& Knežević-Jugović, Z.. (2022). Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves. in Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade Beograd : Srpsko hemijsko društvo = Serbian Chemical Society., 174-174. https://hdl.handle.net/21.15107/rcub_technorep_6252
Mijalković J, Šekuljica N, Biçer F, Pavlović N, Jakovetić Tanasković S, Knežević-Jugović Z. Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves. in Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade. 2022;:174-174. https://hdl.handle.net/21.15107/rcub_technorep_6252 .
Mijalković, Jelena, Šekuljica, Nataša, Biçer, Furkan, Pavlović, Neda, Jakovetić Tanasković, Sonja, Knežević-Jugović, Zorica, "Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves" in Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade (2022):174-174, https://hdl.handle.net/21.15107/rcub_technorep_6252 .