dc.description.abstract | Liposomes, as biodegradable, non-toxic and non-immunogenic drug delivery systems, can
provide controlled release of active molecules and their protection from degradation. Therefore,
the aim of the present study was encapsulation of gentisic acid (antioxidant polyphenols acid)
into liposomes containing phospholipids (Lipoid) and various amounts of β-sitosterol. Liposomal
bilayer containing β-sitosterol was more fluid and flexible, in comparison to pure Lipoid
liposomes. Gentisic acid has caused the rearranging of the acid chains of phospholipids bilayer
in the 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMADPH) environment. Liposomal vesicles containing Lipoid and β-sitosterol have smaller diameter
compared to pure Lipoid, whereas there is no statistically significant difference in polydispersity
index (PDI). Additionally, higher concentration of β-sitosterol has induced the increase of
absolute zeta potential values and consequently higher stability of liposomal suspension. The
addition of various amounts of gentisic acid did not influence the changes of particle size and
PDI values, while in Lipoid liposomes gentisic acid has induced significant and gradual increase
of zeta potential. Lipid peroxidation was higher in Lipoid-β-sitosterol system, particularly in
Lipoid 50%-β-sitosterol 50% sample, whereas gentisic acid has reduced rate of membrane lipid
peroxidation. In comparison to pure Lipoid, Lipoid-β-sitosterol system has shown better
membrane permeability, smaller particle size, higher zeta potential and stability, which made
Lipoid-β-sitosterol liposomes suitable delivery system for gentisic acid. | sr |