Приказ основних података о документу
The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes
dc.creator | Vuković, Goran D. | |
dc.creator | Tomić, Sergej | |
dc.creator | Marinković, Aleksandar | |
dc.creator | Radmilović, Velimir R. | |
dc.creator | Uskoković, Petar | |
dc.creator | Čolić, Miodrag | |
dc.date.accessioned | 2021-03-10T11:19:40Z | |
dc.date.available | 2021-03-10T11:19:40Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 0008-6223 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1648 | |
dc.description.abstract | Dapsone is an anti-microbial and anti-inflammatory drug. Water-dispersible dapsone-modified multi-wall carbon nanotubes (dap-MWCNTs) were prepared by chemical modification of the carboxyl groups introduced on the surface of the nanotubes using O-(7-aza-benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (N-HATU) and N,N-diisopropylethylamine (DIEA). The modification was confirmed by Fourier-transform infrared spectroscopy, transmission election microscopy and thermogravimetric analysis. The biological effect of dap-MWCNTs was tested using rat peritoneal macrophages (PMempty set). By confocal laser microscopy and flow cytometry, it was shown that the dap-MWCNTs were rapidly ingested by PMempty set as were the control, oxidized o-MWCNTs. Neither dap-MWCNTs at lower concentrations (up to 50 mu g/ml), nor o-MWCNTs, at equivalent concentrations, respectively affected the viability of PMempty set, while higher concentrations triggered apoptosis. Apoptosis of PMempty set induced by the control, o-MWCNTs, was higher than that induced by dap-MWCNTs and it correlated with the induction of oxidative stress. In contrast, dap-MWCNTs did not trigger oxidative stress but caused apoptosis of PMempty set predominantly after prolonged cultivation (3 days). Although equivalent concentrations of soluble dapsone induced oxidative stress, they were anti-apoptotic. Cumulatively, the obtained results show the complexity of dap-MWCNT/PMempty set interactions and suggest that this complex could be investigated for the treatment of dapsone-sensitive intracellular microorganisms or inflammatory diseases responding to dapsone therapy. | en |
dc.publisher | Pergamon-Elsevier Science Ltd, Oxford | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/142006/RS// | |
dc.relation | Millitary Medical Academy, Belgrade Serbia [VMA/06-10/A5] | |
dc.relation | US Department of EnergyUnited States Department of Energy (DOE) [DE-ACO2-05CH11231] | |
dc.rights | restrictedAccess | |
dc.source | Carbon | |
dc.title | The response of peritoneal macrophages to dapsone covalently attached on the surface of carbon nanotubes | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 3078 | |
dc.citation.issue | 11 | |
dc.citation.other | 48(11): 3066-3078 | |
dc.citation.rank | aM21 | |
dc.citation.spage | 3066 | |
dc.citation.volume | 48 | |
dc.identifier.doi | 10.1016/j.carbon.2010.04.043 | |
dc.identifier.scopus | 2-s2.0-79955015341 | |
dc.identifier.wos | 000279984600008 | |
dc.type.version | publishedVersion |