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Evaluation of beta-galactosidase from Lactobacillus acidophilus as biocatalyst for galacto-oligosaccharides synthesis: Product structural characterization and enzyme immobilization

Authorized Users Only
2018
Authors
Carević, Milica
Vukašinović-Sekulić, Maja
Ćorović, Marija
Rogniaux, Helene
Ropartz, David
Veličković, Dušan
Bezbradica, Dejan
Article (Published version)
Metadata
Show full item record
Abstract
beta-Galactosidase is an important industrial enzyme that catalyzes reaction of lactose hydrolysis and recently more interesting reaction of transgalactosylation, yielding a highly valuable group of prebiotic compounds named galacto-oligosaccharides (GOS). In this paper, parameters for achieving high yields of tailor-made GOS using crude beta-galactosidase obtained from Lactobacillus acidophilus ATCC 4356, probiotic bacteria regarded as safe for human consumption, were optimized. At the same time, detailed structural elucidation of obtained GOS was conducted, and it was concluded that beta-galactosidase from L acidophilus shows a particular specificity towards the formation of beta-(1 - gt 6) glycosidic bonds. In order to develop more stable and economically cost-effective preparation, crude enzyme was successfully immobilized on a methacrylic polymer carrier Lifetech ECR8409, leading to its simultaneous 2-fold purification. This immobilized preparation showed unchanged specificity to...wards the transgalactosylation reaction, thus yielding 86 WI GOS under the previously optimized conditions (lactose concentration 400 g/l in 0.1 M sodium phosphate buffer, pH 6.8 and temperature 50 degrees C).

Keywords:
beta-Galactosidase / Immobilization / Lactobacillus acidophilus / Galacto-oligosaccharides / Lifetech ECR resins
Source:
Journal of Bioscience and Bioengineering, 2018, 126, 6, 697-704
Publisher:
  • Soc Bioscience Bioengineering Japan, Osaka
Funding / projects:
  • Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)

DOI: 10.1016/j.jbiosc.2018.06.003

ISSN: 1389-1723

PubMed: 30401452

WoS: 000452933700005

Scopus: 2-s2.0-85049305185
[ Google Scholar ]
18
10
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3934
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
Institution/Community
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Carević, Milica
AU  - Vukašinović-Sekulić, Maja
AU  - Ćorović, Marija
AU  - Rogniaux, Helene
AU  - Ropartz, David
AU  - Veličković, Dušan
AU  - Bezbradica, Dejan
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3934
AB  - beta-Galactosidase is an important industrial enzyme that catalyzes reaction of lactose hydrolysis and recently more interesting reaction of transgalactosylation, yielding a highly valuable group of prebiotic compounds named galacto-oligosaccharides (GOS). In this paper, parameters for achieving high yields of tailor-made GOS using crude beta-galactosidase obtained from Lactobacillus acidophilus ATCC 4356, probiotic bacteria regarded as safe for human consumption, were optimized. At the same time, detailed structural elucidation of obtained GOS was conducted, and it was concluded that beta-galactosidase from L acidophilus shows a particular specificity towards the formation of beta-(1 - gt  6) glycosidic bonds. In order to develop more stable and economically cost-effective preparation, crude enzyme was successfully immobilized on a methacrylic polymer carrier Lifetech ECR8409, leading to its simultaneous 2-fold purification. This immobilized preparation showed unchanged specificity towards the transgalactosylation reaction, thus yielding 86 WI GOS under the previously optimized conditions (lactose concentration 400 g/l in 0.1 M sodium phosphate buffer, pH 6.8 and temperature 50 degrees C).
PB  - Soc Bioscience Bioengineering Japan, Osaka
T2  - Journal of Bioscience and Bioengineering
T1  - Evaluation of beta-galactosidase from Lactobacillus acidophilus as biocatalyst for galacto-oligosaccharides synthesis: Product structural characterization and enzyme immobilization
EP  - 704
IS  - 6
SP  - 697
VL  - 126
DO  - 10.1016/j.jbiosc.2018.06.003
ER  - 
@article{
author = "Carević, Milica and Vukašinović-Sekulić, Maja and Ćorović, Marija and Rogniaux, Helene and Ropartz, David and Veličković, Dušan and Bezbradica, Dejan",
year = "2018",
abstract = "beta-Galactosidase is an important industrial enzyme that catalyzes reaction of lactose hydrolysis and recently more interesting reaction of transgalactosylation, yielding a highly valuable group of prebiotic compounds named galacto-oligosaccharides (GOS). In this paper, parameters for achieving high yields of tailor-made GOS using crude beta-galactosidase obtained from Lactobacillus acidophilus ATCC 4356, probiotic bacteria regarded as safe for human consumption, were optimized. At the same time, detailed structural elucidation of obtained GOS was conducted, and it was concluded that beta-galactosidase from L acidophilus shows a particular specificity towards the formation of beta-(1 - gt  6) glycosidic bonds. In order to develop more stable and economically cost-effective preparation, crude enzyme was successfully immobilized on a methacrylic polymer carrier Lifetech ECR8409, leading to its simultaneous 2-fold purification. This immobilized preparation showed unchanged specificity towards the transgalactosylation reaction, thus yielding 86 WI GOS under the previously optimized conditions (lactose concentration 400 g/l in 0.1 M sodium phosphate buffer, pH 6.8 and temperature 50 degrees C).",
publisher = "Soc Bioscience Bioengineering Japan, Osaka",
journal = "Journal of Bioscience and Bioengineering",
title = "Evaluation of beta-galactosidase from Lactobacillus acidophilus as biocatalyst for galacto-oligosaccharides synthesis: Product structural characterization and enzyme immobilization",
pages = "704-697",
number = "6",
volume = "126",
doi = "10.1016/j.jbiosc.2018.06.003"
}
Carević, M., Vukašinović-Sekulić, M., Ćorović, M., Rogniaux, H., Ropartz, D., Veličković, D.,& Bezbradica, D.. (2018). Evaluation of beta-galactosidase from Lactobacillus acidophilus as biocatalyst for galacto-oligosaccharides synthesis: Product structural characterization and enzyme immobilization. in Journal of Bioscience and Bioengineering
Soc Bioscience Bioengineering Japan, Osaka., 126(6), 697-704.
https://doi.org/10.1016/j.jbiosc.2018.06.003
Carević M, Vukašinović-Sekulić M, Ćorović M, Rogniaux H, Ropartz D, Veličković D, Bezbradica D. Evaluation of beta-galactosidase from Lactobacillus acidophilus as biocatalyst for galacto-oligosaccharides synthesis: Product structural characterization and enzyme immobilization. in Journal of Bioscience and Bioengineering. 2018;126(6):697-704.
doi:10.1016/j.jbiosc.2018.06.003 .
Carević, Milica, Vukašinović-Sekulić, Maja, Ćorović, Marija, Rogniaux, Helene, Ropartz, David, Veličković, Dušan, Bezbradica, Dejan, "Evaluation of beta-galactosidase from Lactobacillus acidophilus as biocatalyst for galacto-oligosaccharides synthesis: Product structural characterization and enzyme immobilization" in Journal of Bioscience and Bioengineering, 126, no. 6 (2018):697-704,
https://doi.org/10.1016/j.jbiosc.2018.06.003 . .

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