TY  - CONF
AU  - Zvicer, Jovana
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6955
AB  - Developing new biomaterials for bone tissue engineering is of vital importance due to the increasing demand for bone transplants resulting from various causes, such as trauma, tumors, infection, and genetic conditions. To address this persistent need, biomaterials for bone tissue engineering must fulfil several critical requirements, such as mimicking the hierarchical structure of complex bone tissue composed of organic and inorganic components, possessing suitable mechanical properties, pore size, and porosity, as well as an appropriate degradation rate. Our research group has successfully developed two promising biomaterials for bone tissue engineering, utilizing a combination of bioactive glass (BAG) or β-tricalcium phosphate (β-TCP) - undoped or doped with Mg2+, and different natural polymers, such as gellan gum (GG) and alginate (ALG). Obtained scaffolds were assessed in terms of porosity and pore size distribution, and mechanical properties under physiological levels of mechanical compression (337.5 μm/s loading rate, 10% strain) using a dynamic compression bioreactor coupled with medium perfusion over a 14-day period. The mechanical properties of the β-TCP-ALG scaffolds were influenced by the composition of calcium phosphate fillers, with improvements observed as Ca2+ concentration increased while Mg2+ concentration decreased. These properties were significantly higher compared to the mechanical properties of BAG-GG scaffolds. Bioactivity of the scaffolds was evaluated under static and biomimetic conditions in the perfusion bioreactor, with a continuous flow of simulated body fluid (SBF) at a superficial velocity of 400 µm/s. The formation of hydroxyapatite (HAp) within the BAG-based scaffolds was studied for 14 days, while for β-TCP-based scaffolds, the studies continued for 28 days. The results revealed a remarkable increase in HAp formation under bioreactor conditions compared to static controls in both types of scaffolds, with HAp crystals exhibiting a more uniform distribution and a distinctive cauliflower-like morphology throughout the scaffolds. However, bioactivity was more profound for the BAG-GG scaffolds compared to β-TCP-ALG scaffolds due to additional gelation of alginate around the incorporated fillers. Physiologically relevant characterization with the aid of biomimetic bioreactors has unveiled the promising potential of novel composite scaffolds for bone tissue engineering, while also highlighting the importance of carefully balancing different scaffold characteristics, such as mechanical strength and bioactivity, to develop optimal scaffolds tailored for specific applications.
PB  - Novi Sad : Faculty of Technology
C3  - Programme and The Book of Abstracts / 15th ECerS Conference for Young Scientists in Ceramics, CYSC-2023, October 11-14, 2023, Novi Sad, Serbia
T1  - Development and characterization of composites for bone tissue engineering with the aid of biomimetic bioreactors
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6955
ER  - 

@conference{
author = "Zvicer, Jovana and Stojkovska, Jasmina and Obradović, Bojana",
year = "2023",
abstract = "Developing new biomaterials for bone tissue engineering is of vital importance due to the increasing demand for bone transplants resulting from various causes, such as trauma, tumors, infection, and genetic conditions. To address this persistent need, biomaterials for bone tissue engineering must fulfil several critical requirements, such as mimicking the hierarchical structure of complex bone tissue composed of organic and inorganic components, possessing suitable mechanical properties, pore size, and porosity, as well as an appropriate degradation rate. Our research group has successfully developed two promising biomaterials for bone tissue engineering, utilizing a combination of bioactive glass (BAG) or β-tricalcium phosphate (β-TCP) - undoped or doped with Mg2+, and different natural polymers, such as gellan gum (GG) and alginate (ALG). Obtained scaffolds were assessed in terms of porosity and pore size distribution, and mechanical properties under physiological levels of mechanical compression (337.5 μm/s loading rate, 10% strain) using a dynamic compression bioreactor coupled with medium perfusion over a 14-day period. The mechanical properties of the β-TCP-ALG scaffolds were influenced by the composition of calcium phosphate fillers, with improvements observed as Ca2+ concentration increased while Mg2+ concentration decreased. These properties were significantly higher compared to the mechanical properties of BAG-GG scaffolds. Bioactivity of the scaffolds was evaluated under static and biomimetic conditions in the perfusion bioreactor, with a continuous flow of simulated body fluid (SBF) at a superficial velocity of 400 µm/s. The formation of hydroxyapatite (HAp) within the BAG-based scaffolds was studied for 14 days, while for β-TCP-based scaffolds, the studies continued for 28 days. The results revealed a remarkable increase in HAp formation under bioreactor conditions compared to static controls in both types of scaffolds, with HAp crystals exhibiting a more uniform distribution and a distinctive cauliflower-like morphology throughout the scaffolds. However, bioactivity was more profound for the BAG-GG scaffolds compared to β-TCP-ALG scaffolds due to additional gelation of alginate around the incorporated fillers. Physiologically relevant characterization with the aid of biomimetic bioreactors has unveiled the promising potential of novel composite scaffolds for bone tissue engineering, while also highlighting the importance of carefully balancing different scaffold characteristics, such as mechanical strength and bioactivity, to develop optimal scaffolds tailored for specific applications.",
publisher = "Novi Sad : Faculty of Technology",
journal = "Programme and The Book of Abstracts / 15th ECerS Conference for Young Scientists in Ceramics, CYSC-2023, October 11-14, 2023, Novi Sad, Serbia",
title = "Development and characterization of composites for bone tissue engineering with the aid of biomimetic bioreactors",
pages = "27",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6955"
}

Zvicer, J., Stojkovska, J.,& Obradović, B.. (2023). Development and characterization of composites for bone tissue engineering with the aid of biomimetic bioreactors. in Programme and The Book of Abstracts / 15th ECerS Conference for Young Scientists in Ceramics, CYSC-2023, October 11-14, 2023, Novi Sad, Serbia
Novi Sad : Faculty of Technology., 27.
https://hdl.handle.net/21.15107/rcub_technorep_6955

Zvicer J, Stojkovska J, Obradović B. Development and characterization of composites for bone tissue engineering with the aid of biomimetic bioreactors. in Programme and The Book of Abstracts / 15th ECerS Conference for Young Scientists in Ceramics, CYSC-2023, October 11-14, 2023, Novi Sad, Serbia. 2023;:27.
https://hdl.handle.net/21.15107/rcub_technorep_6955 .

Zvicer, Jovana, Stojkovska, Jasmina, Obradović, Bojana, "Development and characterization of composites for bone tissue engineering with the aid of biomimetic bioreactors" in Programme and The Book of Abstracts / 15th ECerS Conference for Young Scientists in Ceramics, CYSC-2023, October 11-14, 2023, Novi Sad, Serbia (2023):27,
https://hdl.handle.net/21.15107/rcub_technorep_6955 .

TY  - CONF
AU  - Zvicer, Jovana
AU  - Stojkovska, Jasmina
AU  - Milošević, Mia
AU  - Veljović, Đorđe
AU  - Obradović, Bojana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6956
AB  - Development of novel biomaterials for use in biomedical applications requires careful assessment due to the intended interactions with cells and tissues. Understanding biocompatibility, non-toxicity, and capability of promoting desired biological responses requires thorough characterization of biomaterial, including its chemical composition, surface properties, mechanical strength, degradation rate, etc. Traditional in vitro methods for evaluating biomaterials in cell monolayers are convenient but limited by the lack of specific biophysical signals found in vivo, which can lead to unreliable results. This in vitro-in vivo gap can result in the unnecessary sacrifice of a large number of animals for testing purposes. Therefore, there is a need for alternative approaches that beter mimic the in vivo environment and accurately predict the behavior of the biomaterial after implantation. Biomimetic bioreactors are primarily developed for tissue engineering to provide the key biochemical (e.g., nutrients, gases, growth factors) and biophysical signals (e.g., shear stress, hydrostatic pressure, mechanical strains) found in vivo and thus could be indispensable tools in physiologically relevant biomaterial assessment. Our group introduced the application of two biomimetic bioreactors for the physiologically relevant characterization of two types of composite biomaterials aimed for bone and osteochondral tissue engineering. In specific, macroporous composite scaffolds were produced using two natural polymers (gellan gum and alginate) as matrices imitating organic phase of bone tissue with incorporated particulate bioactive glass (BAG) and β-tricalcium phosphate (β-TCP) as hydroxyapatite (HAp) precursors. In addition, in osteochondral scaffolds, gellan gum hydrogel served as a cartilaginous layer on top of the porous composite base. Integrity and mechanical properties of all prepared scaffolds were monitored for 14 days under physiological levels of mechanical compression (up to 10% strain, compression rate 337.5 µm s-1) in a bioreactor with dynamic compression and medium perfusion. Bioactivity and HAp formation within the scaffolds were investigated in a perfusion bioreactor under the flow of simulated body fluid for up to 28 days. The scaffolds were assessed by SEM, EDS, and XRD analyses indicating a significant increase in HAp formation under bioreactor conditions as compared to static controls in all investigated samples. Moreover, the formed HAp crystals were more uniformly distributed throughout the scaffolds showing a more cauliflower-like morphology and thus, indicating potentials for bone/osteochondral tissue engineering applications. The obtained results confirm the high influence of experimental conditions on the outcomes of biomaterial characterization and importance of closely mimicking physiological conditions, thus puting forward biomimetic bioreactors as a means in this direction.
PB  - Davos : AO Research Institute
C3  - ARI Abstracts Periodical
T1  - BIOMIMETIC BIOREACTORS AS A TOOL FOR MORE RELEVANT BIOMATERIAL ASSESSMENT
IS  - Collection 3
SP  - 313
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6956
ER  - 

@conference{
author = "Zvicer, Jovana and Stojkovska, Jasmina and Milošević, Mia and Veljović, Đorđe and Obradović, Bojana",
year = "2023",
abstract = "Development of novel biomaterials for use in biomedical applications requires careful assessment due to the intended interactions with cells and tissues. Understanding biocompatibility, non-toxicity, and capability of promoting desired biological responses requires thorough characterization of biomaterial, including its chemical composition, surface properties, mechanical strength, degradation rate, etc. Traditional in vitro methods for evaluating biomaterials in cell monolayers are convenient but limited by the lack of specific biophysical signals found in vivo, which can lead to unreliable results. This in vitro-in vivo gap can result in the unnecessary sacrifice of a large number of animals for testing purposes. Therefore, there is a need for alternative approaches that beter mimic the in vivo environment and accurately predict the behavior of the biomaterial after implantation. Biomimetic bioreactors are primarily developed for tissue engineering to provide the key biochemical (e.g., nutrients, gases, growth factors) and biophysical signals (e.g., shear stress, hydrostatic pressure, mechanical strains) found in vivo and thus could be indispensable tools in physiologically relevant biomaterial assessment. Our group introduced the application of two biomimetic bioreactors for the physiologically relevant characterization of two types of composite biomaterials aimed for bone and osteochondral tissue engineering. In specific, macroporous composite scaffolds were produced using two natural polymers (gellan gum and alginate) as matrices imitating organic phase of bone tissue with incorporated particulate bioactive glass (BAG) and β-tricalcium phosphate (β-TCP) as hydroxyapatite (HAp) precursors. In addition, in osteochondral scaffolds, gellan gum hydrogel served as a cartilaginous layer on top of the porous composite base. Integrity and mechanical properties of all prepared scaffolds were monitored for 14 days under physiological levels of mechanical compression (up to 10% strain, compression rate 337.5 µm s-1) in a bioreactor with dynamic compression and medium perfusion. Bioactivity and HAp formation within the scaffolds were investigated in a perfusion bioreactor under the flow of simulated body fluid for up to 28 days. The scaffolds were assessed by SEM, EDS, and XRD analyses indicating a significant increase in HAp formation under bioreactor conditions as compared to static controls in all investigated samples. Moreover, the formed HAp crystals were more uniformly distributed throughout the scaffolds showing a more cauliflower-like morphology and thus, indicating potentials for bone/osteochondral tissue engineering applications. The obtained results confirm the high influence of experimental conditions on the outcomes of biomaterial characterization and importance of closely mimicking physiological conditions, thus puting forward biomimetic bioreactors as a means in this direction.",
publisher = "Davos : AO Research Institute",
journal = "ARI Abstracts Periodical",
title = "BIOMIMETIC BIOREACTORS AS A TOOL FOR MORE RELEVANT BIOMATERIAL ASSESSMENT",
number = "Collection 3",
pages = "313",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6956"
}

Zvicer, J., Stojkovska, J., Milošević, M., Veljović, Đ.,& Obradović, B.. (2023). BIOMIMETIC BIOREACTORS AS A TOOL FOR MORE RELEVANT BIOMATERIAL ASSESSMENT. in ARI Abstracts Periodical
Davos : AO Research Institute.(Collection 3), 313.
https://hdl.handle.net/21.15107/rcub_technorep_6956

Zvicer J, Stojkovska J, Milošević M, Veljović Đ, Obradović B. BIOMIMETIC BIOREACTORS AS A TOOL FOR MORE RELEVANT BIOMATERIAL ASSESSMENT. in ARI Abstracts Periodical. 2023;(Collection 3):313.
https://hdl.handle.net/21.15107/rcub_technorep_6956 .

Zvicer, Jovana, Stojkovska, Jasmina, Milošević, Mia, Veljović, Đorđe, Obradović, Bojana, "BIOMIMETIC BIOREACTORS AS A TOOL FOR MORE RELEVANT BIOMATERIAL ASSESSMENT" in ARI Abstracts Periodical, no. Collection 3 (2023):313,
https://hdl.handle.net/21.15107/rcub_technorep_6956 .

TY  - CONF
AU  - Zvicer, Jovana
AU  - Stojkovska, Jasmina
AU  - Veljović, Đorđe
AU  - Milošević, Mia
AU  - Obradović, Bojana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6957
AB  - Comprehensive preclinical studies are essential for the development of novel biomaterials that can be used in biomedical applications. However, traditional methods used for the evaluation of biomaterials have certain limitations. In vitro testing in cell monolayers is fast and easily accessible, but the 2D environment can affect cell metabolism and morphology, leading to unreliable results. On the other hand, in vivo animal studies are complex, time-consuming, expensive, and raise ethical concerns. Biomimetic bioreactors, primarily developed for tissue engineering to provide a physiologically relevant, strictly controlled environment that mimics the conditions in specific tissues or organs, could be indispensable tools in physiologically relevant biomaterial characterization and step between in vitro and in vivo studies. They offer the majority or all the necessary biochemical (e.g. pH, nutrients, gases, growth factors) and biophysical signals (e.g., shear stress, hydrostatic pressure, mechanical strains) highly relevant for biomaterial assessment and prediction of material behavior after implantation. Our group has developed two types of potential biomaterials aimed for bone and osteochondral tissue engineering based on bioactive glass (BAG), β-tricalcium phosphate (β-TCP), and different natural polymers (gellan gum and alginate). Scaffolds' integrity and mechanical properties were monitored continuously under the physiological level of mechanical compression using a dynamic compression bioreactor coupled with medium perfusion during 14 days. Formation of hydroxyapatite (HAp) within the scaffolds was investigated in a perfusion bioreactor, in the presence of simulated body fluid (SBF) during 14 and 28 days for scaffolds based on BAG and β-TCP, respectively. SEM, EDS, and XRD results have shown a significant increase in the formation of HAp under bioreactor conditions compared to static control conditions. Beyond that, formed HAp crystals were more uniformly distributed throughout scaffolds and presented more cauliflower-like morphology. The obtained results demonstrated the utilization potential of biomimetic bioreactors in physiologically relevant biomaterial characterization.
C3  - Book of Abstract / 3rd YCN Workshop, 19th to 21st April 2023, Aveiro, Portugal
T1  - HOW NOVEL BIOMATERIALS BASED ON BIOACTIVE GLASS AND β-TRICALCIUM PHOSPHATE CAN BE EVALUATED UNDER PHYSIOLOGICALLY RELEVANT CONDITIONS?
SP  - 23
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6957
ER  - 

@conference{
author = "Zvicer, Jovana and Stojkovska, Jasmina and Veljović, Đorđe and Milošević, Mia and Obradović, Bojana",
year = "2023",
abstract = "Comprehensive preclinical studies are essential for the development of novel biomaterials that can be used in biomedical applications. However, traditional methods used for the evaluation of biomaterials have certain limitations. In vitro testing in cell monolayers is fast and easily accessible, but the 2D environment can affect cell metabolism and morphology, leading to unreliable results. On the other hand, in vivo animal studies are complex, time-consuming, expensive, and raise ethical concerns. Biomimetic bioreactors, primarily developed for tissue engineering to provide a physiologically relevant, strictly controlled environment that mimics the conditions in specific tissues or organs, could be indispensable tools in physiologically relevant biomaterial characterization and step between in vitro and in vivo studies. They offer the majority or all the necessary biochemical (e.g. pH, nutrients, gases, growth factors) and biophysical signals (e.g., shear stress, hydrostatic pressure, mechanical strains) highly relevant for biomaterial assessment and prediction of material behavior after implantation. Our group has developed two types of potential biomaterials aimed for bone and osteochondral tissue engineering based on bioactive glass (BAG), β-tricalcium phosphate (β-TCP), and different natural polymers (gellan gum and alginate). Scaffolds' integrity and mechanical properties were monitored continuously under the physiological level of mechanical compression using a dynamic compression bioreactor coupled with medium perfusion during 14 days. Formation of hydroxyapatite (HAp) within the scaffolds was investigated in a perfusion bioreactor, in the presence of simulated body fluid (SBF) during 14 and 28 days for scaffolds based on BAG and β-TCP, respectively. SEM, EDS, and XRD results have shown a significant increase in the formation of HAp under bioreactor conditions compared to static control conditions. Beyond that, formed HAp crystals were more uniformly distributed throughout scaffolds and presented more cauliflower-like morphology. The obtained results demonstrated the utilization potential of biomimetic bioreactors in physiologically relevant biomaterial characterization.",
journal = "Book of Abstract / 3rd YCN Workshop, 19th to 21st April 2023, Aveiro, Portugal",
title = "HOW NOVEL BIOMATERIALS BASED ON BIOACTIVE GLASS AND β-TRICALCIUM PHOSPHATE CAN BE EVALUATED UNDER PHYSIOLOGICALLY RELEVANT CONDITIONS?",
pages = "23",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6957"
}

Zvicer, J., Stojkovska, J., Veljović, Đ., Milošević, M.,& Obradović, B.. (2023). HOW NOVEL BIOMATERIALS BASED ON BIOACTIVE GLASS AND β-TRICALCIUM PHOSPHATE CAN BE EVALUATED UNDER PHYSIOLOGICALLY RELEVANT CONDITIONS?. in Book of Abstract / 3rd YCN Workshop, 19th to 21st April 2023, Aveiro, Portugal, 23.
https://hdl.handle.net/21.15107/rcub_technorep_6957

Zvicer J, Stojkovska J, Veljović Đ, Milošević M, Obradović B. HOW NOVEL BIOMATERIALS BASED ON BIOACTIVE GLASS AND β-TRICALCIUM PHOSPHATE CAN BE EVALUATED UNDER PHYSIOLOGICALLY RELEVANT CONDITIONS?. in Book of Abstract / 3rd YCN Workshop, 19th to 21st April 2023, Aveiro, Portugal. 2023;:23.
https://hdl.handle.net/21.15107/rcub_technorep_6957 .

Zvicer, Jovana, Stojkovska, Jasmina, Veljović, Đorđe, Milošević, Mia, Obradović, Bojana, "HOW NOVEL BIOMATERIALS BASED ON BIOACTIVE GLASS AND β-TRICALCIUM PHOSPHATE CAN BE EVALUATED UNDER PHYSIOLOGICALLY RELEVANT CONDITIONS?" in Book of Abstract / 3rd YCN Workshop, 19th to 21st April 2023, Aveiro, Portugal (2023):23,
https://hdl.handle.net/21.15107/rcub_technorep_6957 .

TY  - CONF
AU  - Zvicer, Jovana
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6958
AB  - Development of novel biomaterials for potential biomedical applications requires comprehensive preclinical studies. Traditional methods for biomaterial evaluation are based on in vitro testing routinely performed in monolayer cell cultures followed by in vivo animal studies. However, these methods have numerous limitations. Although studies in cell monolayers allow rapid evaluation of biomaterials by standardized protocols, quantitative and comparable results, cell metabolism and morphology is changed in the 2-dimensional environment often leading to unreliable results. On other hand, animal studies are complex, time-consuming, expensive and raise ethical concerns. One of the approaches to address this problem and obtain reliable results in a more efficient way is utilization of biomimetic bioreactors. These bioreactors are primarily developed as an essential component in tissue engineering mimicking physiological in vivo conditions in particular tissue or organ by providing all necessary biochemical (e.g. pH, nutrients, gases, growth factors) and biophysical signals (e.g., shear stress, hydrostatic pressure, mechanical strains) for cell differentiation and metabolic activity. Some examples include perfusion bioreactors, bioreactors with shear stresses and/or dynamic compression, and bioreactor with stretch and shear stresses imitating conditions in vascularized tissues, articular cartilage, and vascular grafts, respectively. Such physiologically relevant, while strictly controlled environment is also beneficial for biomaterial assessment, investigation of cell-biomaterial interactions, and prediction of biomaterial behaviour upon application. The present review provides readers with up-to-date studies and results regarding utilization of biomimetic bioreactors as tools for comprehensive and efficient evaluation of novel biomaterials, such as determination of mechanical characteristics, release of bioactive substances, cell-biomaterial interactions and cytotoxicity.
PB  - Belgrade : Innovation Center of Faculty of Mechanical Engineering
C3  - Programme and The Book of Abstracts / International Conference of Experimental and Numerical Investigations and New Technologies - CNN TECH 2022, 05-08 July 2022, Zlatibor, Serbia
T1  - BIOMIMETIC BIOREACTORS IN CHARACTERIZATION OF NOVEL BIOMATERIALS
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6958
ER  - 

@conference{
author = "Zvicer, Jovana and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
abstract = "Development of novel biomaterials for potential biomedical applications requires comprehensive preclinical studies. Traditional methods for biomaterial evaluation are based on in vitro testing routinely performed in monolayer cell cultures followed by in vivo animal studies. However, these methods have numerous limitations. Although studies in cell monolayers allow rapid evaluation of biomaterials by standardized protocols, quantitative and comparable results, cell metabolism and morphology is changed in the 2-dimensional environment often leading to unreliable results. On other hand, animal studies are complex, time-consuming, expensive and raise ethical concerns. One of the approaches to address this problem and obtain reliable results in a more efficient way is utilization of biomimetic bioreactors. These bioreactors are primarily developed as an essential component in tissue engineering mimicking physiological in vivo conditions in particular tissue or organ by providing all necessary biochemical (e.g. pH, nutrients, gases, growth factors) and biophysical signals (e.g., shear stress, hydrostatic pressure, mechanical strains) for cell differentiation and metabolic activity. Some examples include perfusion bioreactors, bioreactors with shear stresses and/or dynamic compression, and bioreactor with stretch and shear stresses imitating conditions in vascularized tissues, articular cartilage, and vascular grafts, respectively. Such physiologically relevant, while strictly controlled environment is also beneficial for biomaterial assessment, investigation of cell-biomaterial interactions, and prediction of biomaterial behaviour upon application. The present review provides readers with up-to-date studies and results regarding utilization of biomimetic bioreactors as tools for comprehensive and efficient evaluation of novel biomaterials, such as determination of mechanical characteristics, release of bioactive substances, cell-biomaterial interactions and cytotoxicity.",
publisher = "Belgrade : Innovation Center of Faculty of Mechanical Engineering",
journal = "Programme and The Book of Abstracts / International Conference of Experimental and Numerical Investigations and New Technologies - CNN TECH 2022, 05-08 July 2022, Zlatibor, Serbia",
title = "BIOMIMETIC BIOREACTORS IN CHARACTERIZATION OF NOVEL BIOMATERIALS",
pages = "17",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6958"
}

Zvicer, J., Stojkovska, J.,& Obradović, B.. (2022). BIOMIMETIC BIOREACTORS IN CHARACTERIZATION OF NOVEL BIOMATERIALS. in Programme and The Book of Abstracts / International Conference of Experimental and Numerical Investigations and New Technologies - CNN TECH 2022, 05-08 July 2022, Zlatibor, Serbia
Belgrade : Innovation Center of Faculty of Mechanical Engineering., 17.
https://hdl.handle.net/21.15107/rcub_technorep_6958

Zvicer J, Stojkovska J, Obradović B. BIOMIMETIC BIOREACTORS IN CHARACTERIZATION OF NOVEL BIOMATERIALS. in Programme and The Book of Abstracts / International Conference of Experimental and Numerical Investigations and New Technologies - CNN TECH 2022, 05-08 July 2022, Zlatibor, Serbia. 2022;:17.
https://hdl.handle.net/21.15107/rcub_technorep_6958 .

Zvicer, Jovana, Stojkovska, Jasmina, Obradović, Bojana, "BIOMIMETIC BIOREACTORS IN CHARACTERIZATION OF NOVEL BIOMATERIALS" in Programme and The Book of Abstracts / International Conference of Experimental and Numerical Investigations and New Technologies - CNN TECH 2022, 05-08 July 2022, Zlatibor, Serbia (2022):17,
https://hdl.handle.net/21.15107/rcub_technorep_6958 .

TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Zvicer, Jovana
AU  - Andrejevic, Milica
AU  - Janaćković, Đorđe
AU  - Obradović, Bojana
AU  - Veljović, Đorđe
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4934
AB  - In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure. HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg2+ were HAP and beta-tricalcium phosphate, respectively. Upon mixing with Na-alginate, followed by gelation and freeze-dying, highly macroporous composite scaffolds were obtained with open and connected pores and uniformly dispersed mineral phase as determined by scanning electron microscopy. Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca2+ concentration increased while Mg2+ concentration decreased. HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor. The continuous SBF flow (superficial velocity of 400 mu m/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds. These findings indicated potentials of novel biomimetic scaffolds for use in bone tissue engineering.
T2  - Journal of Biomedical Materials Research Part B-Applied Biomaterials
T1  - Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions
EP  - 2090
IS  - 12
SP  - 2079
VL  - 109
DO  - 10.1002/jbm.b.34856
ER  - 

@article{
author = "Stojkovska, Jasmina and Zvicer, Jovana and Andrejevic, Milica and Janaćković, Đorđe and Obradović, Bojana and Veljović, Đorđe",
year = "2021",
abstract = "In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure. HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg2+ were HAP and beta-tricalcium phosphate, respectively. Upon mixing with Na-alginate, followed by gelation and freeze-dying, highly macroporous composite scaffolds were obtained with open and connected pores and uniformly dispersed mineral phase as determined by scanning electron microscopy. Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca2+ concentration increased while Mg2+ concentration decreased. HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor. The continuous SBF flow (superficial velocity of 400 mu m/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds. These findings indicated potentials of novel biomimetic scaffolds for use in bone tissue engineering.",
journal = "Journal of Biomedical Materials Research Part B-Applied Biomaterials",
title = "Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions",
pages = "2090-2079",
number = "12",
volume = "109",
doi = "10.1002/jbm.b.34856"
}

Stojkovska, J., Zvicer, J., Andrejevic, M., Janaćković, Đ., Obradović, B.,& Veljović, Đ.. (2021). Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions. in Journal of Biomedical Materials Research Part B-Applied Biomaterials, 109(12), 2079-2090.
https://doi.org/10.1002/jbm.b.34856

Stojkovska J, Zvicer J, Andrejevic M, Janaćković Đ, Obradović B, Veljović Đ. Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions. in Journal of Biomedical Materials Research Part B-Applied Biomaterials. 2021;109(12):2079-2090.
doi:10.1002/jbm.b.34856 .

Stojkovska, Jasmina, Zvicer, Jovana, Andrejevic, Milica, Janaćković, Đorđe, Obradović, Bojana, Veljović, Đorđe, "Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions" in Journal of Biomedical Materials Research Part B-Applied Biomaterials, 109, no. 12 (2021):2079-2090,
https://doi.org/10.1002/jbm.b.34856 . .

TY  - CONF
AU  - Zvicer, Jovana
AU  - Radonjić, Mia
AU  - Kovrlija, Ilijana
AU  - Medić, Ana
AU  - Novak, Saša
AU  - Obradović, Bojana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6352
AB  - Bilayer scaffolds based on gellan gum (GG) and nanoparticulate bioactive-glass (BAG) were developed by an integrative
approach based on engineering principles and characterization in biomimetic bioreactors. The osteo-inductive GG-BAG
layer containing 2 % w/w GG and 2 % w/w BAG (composition: 70 n/n % SiO2, 30 n/n % CaO) was produced by gelation
followed by freeze-drying to obtain open porosity in axial and radial directions. The chondral layer was obtained by
dispensing a warm 2 % w/w GG solution at 60˚C over the frozen macroporous GG-BAG layer at -25˚C. The temperatures
were optimized by applying a one-dimensional unsteady-state heat transfer model so to obtain a thin integration zone, 0.5
– 1 mm thick. The scaffolds were evaluated regarding bioactivity in a biomimetic bioreactor with specially designed
chambers to provide supply of two media relevant for chondral and bone tissues. In the present experiment, simulated
body fluid (SBF) was supplied countercurrently continuously during 14 days of the experiment (1.1 ml min-1 flowrate), while
dynamic compression (5 % deformation, 0.68 Hz frequency, 337.5 µm s-1 loading rate, 1 h / day) was applied on the
chondral layer, from day 7 to day 14. SEM analyses have confirmed the retained integrity of the scaffolds, as well as
formation of hydroxyapatite (HAp) uniformly throughout the osteo-layer of the scaffolds.Significantly higher bioactivity
under biomimetic conditions compared to static controls resulted in slightly but significantly increased compression
modulus. These results indicated a high potential of the applied integrative strategy for the development of biomimetic
bilayer scaffolds.
C3  - 6th World TERMIS Conference 2021 Abstract Book
T1  - An integrative approach in developing scaffolds based on gellan gum and bioactive glass aimed for osteochondral tissue engineering
SP  - 663
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6352
ER  - 

@conference{
author = "Zvicer, Jovana and Radonjić, Mia and Kovrlija, Ilijana and Medić, Ana and Novak, Saša and Obradović, Bojana",
year = "2021",
abstract = "Bilayer scaffolds based on gellan gum (GG) and nanoparticulate bioactive-glass (BAG) were developed by an integrative
approach based on engineering principles and characterization in biomimetic bioreactors. The osteo-inductive GG-BAG
layer containing 2 % w/w GG and 2 % w/w BAG (composition: 70 n/n % SiO2, 30 n/n % CaO) was produced by gelation
followed by freeze-drying to obtain open porosity in axial and radial directions. The chondral layer was obtained by
dispensing a warm 2 % w/w GG solution at 60˚C over the frozen macroporous GG-BAG layer at -25˚C. The temperatures
were optimized by applying a one-dimensional unsteady-state heat transfer model so to obtain a thin integration zone, 0.5
– 1 mm thick. The scaffolds were evaluated regarding bioactivity in a biomimetic bioreactor with specially designed
chambers to provide supply of two media relevant for chondral and bone tissues. In the present experiment, simulated
body fluid (SBF) was supplied countercurrently continuously during 14 days of the experiment (1.1 ml min-1 flowrate), while
dynamic compression (5 % deformation, 0.68 Hz frequency, 337.5 µm s-1 loading rate, 1 h / day) was applied on the
chondral layer, from day 7 to day 14. SEM analyses have confirmed the retained integrity of the scaffolds, as well as
formation of hydroxyapatite (HAp) uniformly throughout the osteo-layer of the scaffolds.Significantly higher bioactivity
under biomimetic conditions compared to static controls resulted in slightly but significantly increased compression
modulus. These results indicated a high potential of the applied integrative strategy for the development of biomimetic
bilayer scaffolds.",
journal = "6th World TERMIS Conference 2021 Abstract Book",
title = "An integrative approach in developing scaffolds based on gellan gum and bioactive glass aimed for osteochondral tissue engineering",
pages = "663",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6352"
}

Zvicer, J., Radonjić, M., Kovrlija, I., Medić, A., Novak, S.,& Obradović, B.. (2021). An integrative approach in developing scaffolds based on gellan gum and bioactive glass aimed for osteochondral tissue engineering. in 6th World TERMIS Conference 2021 Abstract Book, 663.
https://hdl.handle.net/21.15107/rcub_technorep_6352

Zvicer J, Radonjić M, Kovrlija I, Medić A, Novak S, Obradović B. An integrative approach in developing scaffolds based on gellan gum and bioactive glass aimed for osteochondral tissue engineering. in 6th World TERMIS Conference 2021 Abstract Book. 2021;:663.
https://hdl.handle.net/21.15107/rcub_technorep_6352 .

Zvicer, Jovana, Radonjić, Mia, Kovrlija, Ilijana, Medić, Ana, Novak, Saša, Obradović, Bojana, "An integrative approach in developing scaffolds based on gellan gum and bioactive glass aimed for osteochondral tissue engineering" in 6th World TERMIS Conference 2021 Abstract Book (2021):663,
https://hdl.handle.net/21.15107/rcub_technorep_6352 .

TY  - THES
AU  - Zvicer, Jovana S.
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6348
AB  - Cilj istraživanja u ovoj doktorskoj disertaciji je bila primena biomimičnih bioreaktora zasveobuhvatnu i fiziološki relevantnu karakterizaciju novih bioaktivnih biomaterijala za potencijalnuprimenu u inženjerstvu tkiva, a time i razvoj novih metodologija za pouzdanu evaluacijubiomaterijala. Ispitani su nanokompozitni hidrogelovi alginata sa nanočesticama srebra zainženjerstvo tkiva artikularne hrskavice i hidrogelovi gelanske gume sa nanočesticama bioaktivnogstakla za inženjerstvo tkiva kosti i osteohondralnog tkiva primenom dva biomimična bioreaktora:protočnog i bioreaktora sa dinamičkom kompresijom. Poseban cilj ove disertacije je bio i razvojnovog bioreaktora za inženjerstvo tkiva intervertebralnog diska.Ispitivanja nanokompozitnih Ag/alginatih hidrogelova baziranih na dve vrste alginatapokazala su da polazni sastav alginata utiče na veličinu i kinetiku otpuštanja nanočestica srebra imehaničke karakteristike hidrogelova u fiziološki relevantnim bioreaktorskim uslovima, na osnovukojih je određena njihova potencijalna primena. Kinetika otpuštanja srebra je uspešno modelovanaprenosom mase difuzijom i konvekcijom.Makroporozni hidrogelovi na bazi gelanske gume i bioaktivnog stakla sa otvorenim poramasu dobijeni unapređenom procedurom, a u fiziološki relevantnim uslovima u protočnom bioreaktorudošlo je do značajnog formiranja kalcijum fosfata i poboljšanja mehaničkih karakteristika.Razvijena je i jednostavna metoda za dobijanje dvofaznih osteohondralnih implantata sa dobromintegracijom između slojeva.Rezultati dobijeni u ovoj doktorskoj disertaciji su potvrdili značaj i široki potencijal primenebiomimičnih bioreaktora i sprovedene metodologije u više naučnih oblasti, od razvoja novihbiomaterijala i inženjerstva tkiva do nanotoksikologije i biologije ćelija.
AB  - The aim of this doctoral dissertation was application of biomimetic bioreactors forcomprehensive and physiologically relevant characterization of novel, bioactive biomaterialsintended for tissue engineering (TE), and, consequently development of new methodologies forreliable biomaterial evaluation, as well. The focus was on nanocomposite alginate hydrogels withsilver nanoparticles (AgNPs) for articular cartilage TE and gellan gum hydrogels with bioactiveglass nanoparticles (GG-BAG) for bone and osteochondral TE, while two biomimetic bioreactorswere applied: perfusion and a bioreactor with dynamic compression. The specific goal of thisdissertation was also development of a novel bioreactor for intervertebral disc TE.Investigation of nanocomposite Ag/alginate hydrogels based on two alginate types hasshown that the initial alginate composition affects the size and release kinetics of AgNPs, as well ashydrogel mechanical properties under physiologically relevant bioreactor conditions, whichindicated the potential use for each alginate hydrogel type. The silver release kinetics wassuccessfully modeled by mass transfer by diffusion and advection-diffusion.Macroporous GG-BAG hydrogels with open pores were obtained by a novel procedure.Physiologically relevant conditions in perfusion bioreactors enhanced calcium phosphate formationand improved hydrogel mechanical properties. Also, a simple method was developed for obtainingbiphasic osteochondral implants with good integration between layers.The obtained results indicate the significance and vast potentials of biomimetic bioreactorsand methodologies developed in this doctoral dissertation for applications in different scientificfields from biomaterials science and TE to nanotoxicology and cell biology.
PB  - Универзитет у Београду, Технолошко-металуршки факултет
T2  - Универзитет у Београду
T1  - Primena biomimičnih bioreaktora u dizajniranju i karakterizaciji novih biomaterijala za inženjerstvo tkiva
UR  - https://hdl.handle.net/21.15107/rcub_nardus_18301
ER  - 

@phdthesis{
author = "Zvicer, Jovana S.",
year = "2020",
abstract = "Cilj istraživanja u ovoj doktorskoj disertaciji je bila primena biomimičnih bioreaktora zasveobuhvatnu i fiziološki relevantnu karakterizaciju novih bioaktivnih biomaterijala za potencijalnuprimenu u inženjerstvu tkiva, a time i razvoj novih metodologija za pouzdanu evaluacijubiomaterijala. Ispitani su nanokompozitni hidrogelovi alginata sa nanočesticama srebra zainženjerstvo tkiva artikularne hrskavice i hidrogelovi gelanske gume sa nanočesticama bioaktivnogstakla za inženjerstvo tkiva kosti i osteohondralnog tkiva primenom dva biomimična bioreaktora:protočnog i bioreaktora sa dinamičkom kompresijom. Poseban cilj ove disertacije je bio i razvojnovog bioreaktora za inženjerstvo tkiva intervertebralnog diska.Ispitivanja nanokompozitnih Ag/alginatih hidrogelova baziranih na dve vrste alginatapokazala su da polazni sastav alginata utiče na veličinu i kinetiku otpuštanja nanočestica srebra imehaničke karakteristike hidrogelova u fiziološki relevantnim bioreaktorskim uslovima, na osnovukojih je određena njihova potencijalna primena. Kinetika otpuštanja srebra je uspešno modelovanaprenosom mase difuzijom i konvekcijom.Makroporozni hidrogelovi na bazi gelanske gume i bioaktivnog stakla sa otvorenim poramasu dobijeni unapređenom procedurom, a u fiziološki relevantnim uslovima u protočnom bioreaktorudošlo je do značajnog formiranja kalcijum fosfata i poboljšanja mehaničkih karakteristika.Razvijena je i jednostavna metoda za dobijanje dvofaznih osteohondralnih implantata sa dobromintegracijom između slojeva.Rezultati dobijeni u ovoj doktorskoj disertaciji su potvrdili značaj i široki potencijal primenebiomimičnih bioreaktora i sprovedene metodologije u više naučnih oblasti, od razvoja novihbiomaterijala i inženjerstva tkiva do nanotoksikologije i biologije ćelija., The aim of this doctoral dissertation was application of biomimetic bioreactors forcomprehensive and physiologically relevant characterization of novel, bioactive biomaterialsintended for tissue engineering (TE), and, consequently development of new methodologies forreliable biomaterial evaluation, as well. The focus was on nanocomposite alginate hydrogels withsilver nanoparticles (AgNPs) for articular cartilage TE and gellan gum hydrogels with bioactiveglass nanoparticles (GG-BAG) for bone and osteochondral TE, while two biomimetic bioreactorswere applied: perfusion and a bioreactor with dynamic compression. The specific goal of thisdissertation was also development of a novel bioreactor for intervertebral disc TE.Investigation of nanocomposite Ag/alginate hydrogels based on two alginate types hasshown that the initial alginate composition affects the size and release kinetics of AgNPs, as well ashydrogel mechanical properties under physiologically relevant bioreactor conditions, whichindicated the potential use for each alginate hydrogel type. The silver release kinetics wassuccessfully modeled by mass transfer by diffusion and advection-diffusion.Macroporous GG-BAG hydrogels with open pores were obtained by a novel procedure.Physiologically relevant conditions in perfusion bioreactors enhanced calcium phosphate formationand improved hydrogel mechanical properties. Also, a simple method was developed for obtainingbiphasic osteochondral implants with good integration between layers.The obtained results indicate the significance and vast potentials of biomimetic bioreactorsand methodologies developed in this doctoral dissertation for applications in different scientificfields from biomaterials science and TE to nanotoxicology and cell biology.",
publisher = "Универзитет у Београду, Технолошко-металуршки факултет",
journal = "Универзитет у Београду",
title = "Primena biomimičnih bioreaktora u dizajniranju i karakterizaciji novih biomaterijala za inženjerstvo tkiva",
url = "https://hdl.handle.net/21.15107/rcub_nardus_18301"
}

Zvicer, J. S.. (2020). Primena biomimičnih bioreaktora u dizajniranju i karakterizaciji novih biomaterijala za inženjerstvo tkiva. in Универзитет у Београду
Универзитет у Београду, Технолошко-металуршки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_18301

Zvicer JS. Primena biomimičnih bioreaktora u dizajniranju i karakterizaciji novih biomaterijala za inženjerstvo tkiva. in Универзитет у Београду. 2020;.
https://hdl.handle.net/21.15107/rcub_nardus_18301 .

Zvicer, Jovana S., "Primena biomimičnih bioreaktora u dizajniranju i karakterizaciji novih biomaterijala za inženjerstvo tkiva" in Универзитет у Београду (2020),
https://hdl.handle.net/21.15107/rcub_nardus_18301 .

TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Zvicer, Jovana
AU  - Milivojević, Milena
AU  - Petrović, Isidora
AU  - Stevanović, Milena
AU  - Obradović, Bojana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4373
AB  - Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Validation of a novel perfusion bioreactor system in cancer research
EP  - 196
IS  - 3
SP  - 187
VL  - 74
DO  - 10.2298/HEMIND200329015S
ER  - 

@article{
author = "Stojkovska, Jasmina and Zvicer, Jovana and Milivojević, Milena and Petrović, Isidora and Stevanović, Milena and Obradović, Bojana",
year = "2020",
abstract = "Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Validation of a novel perfusion bioreactor system in cancer research",
pages = "196-187",
number = "3",
volume = "74",
doi = "10.2298/HEMIND200329015S"
}

Stojkovska, J., Zvicer, J., Milivojević, M., Petrović, I., Stevanović, M.,& Obradović, B.. (2020). Validation of a novel perfusion bioreactor system in cancer research. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 74(3), 187-196.
https://doi.org/10.2298/HEMIND200329015S

Stojkovska J, Zvicer J, Milivojević M, Petrović I, Stevanović M, Obradović B. Validation of a novel perfusion bioreactor system in cancer research. in Hemijska industrija. 2020;74(3):187-196.
doi:10.2298/HEMIND200329015S .

Stojkovska, Jasmina, Zvicer, Jovana, Milivojević, Milena, Petrović, Isidora, Stevanović, Milena, Obradović, Bojana, "Validation of a novel perfusion bioreactor system in cancer research" in Hemijska industrija, 74, no. 3 (2020):187-196,
https://doi.org/10.2298/HEMIND200329015S . .

TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Zvicer, Jovana
AU  - Obradović, Bojana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4451
AB  - Nanocomposite hydrogels that contain silver nanoparticles (AgNPs) are especially attractive for various biomedical applications (e.g., antimicrobial wound dressings, coatings and soft tissue implants) due to strong antimicrobial activity of released silver nanoparticles and/or ions over prolonged times. However, all potential biomedical products have to be thoroughly specified fulfilling strict safety requirements. Characterization of nanocomposites is additionally complicated due to potential harmful effects of nanoparticles and accumulation in cells and tissues. This paper summarizes methods for preclinical characterization of hydrogel nanocomposites containing AgNPs with the particular attention on Ag/alginate hydrogels. Standard physicochemical characterization methods include transmission electron microscopy (TEM), scanning electron microscopy (SEM) and atomic force microscopy (AFM), UV-visible spectroscopy, and Fourier transform infrared spectroscopy (FTIR). Functional in vitro characterization relies on different methods for estimation of silver release, antimicrobial activity, and nanocomposite cytotoxicity. Here, we specially focus on utilization of 3D bioreactor systems that mimic native physiological environments with the aim to reliably predict nanocomposite behavior during implementation and so to decrease the need for animal experimentation. These systems were shown to provide more accurate and relevant data on silver release and cytotoxicity as compared to static systems such as 2D cell monolayer cultures. Finally, nanocomposites are evaluated in vivo in different animal models, which are in the case of wound dressings typically mice, rats, and pigs. The present review provides a basis for defining a strategy for comprehensive and efficient preclinical characterization of novel nanocomposites attractive not only for those containing AgNPs but also other metallic nanoparticles aimed for biomedical applications. Key points center dot A platform for devising comprehensive preclinical evaluation of nanocomposites. center dot Biomimetic bioreactors provide reliable functional nanocomposite evaluation. center dot Cells in 2D cultures are more sensitive to silver nanoparticles than in 3D cultures. center dot Biomimetic bioreactor 3D cell/tissue cultures can address the in vitro-in vivo gap.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Preclinical functional characterization methods of nanocomposite hydrogels containing silver nanoparticles for biomedical applications
EP  - 4658
IS  - 11
SP  - 4643
VL  - 104
DO  - 10.1007/s00253-020-10521-2
ER  - 

@article{
author = "Stojkovska, Jasmina and Zvicer, Jovana and Obradović, Bojana",
year = "2020",
abstract = "Nanocomposite hydrogels that contain silver nanoparticles (AgNPs) are especially attractive for various biomedical applications (e.g., antimicrobial wound dressings, coatings and soft tissue implants) due to strong antimicrobial activity of released silver nanoparticles and/or ions over prolonged times. However, all potential biomedical products have to be thoroughly specified fulfilling strict safety requirements. Characterization of nanocomposites is additionally complicated due to potential harmful effects of nanoparticles and accumulation in cells and tissues. This paper summarizes methods for preclinical characterization of hydrogel nanocomposites containing AgNPs with the particular attention on Ag/alginate hydrogels. Standard physicochemical characterization methods include transmission electron microscopy (TEM), scanning electron microscopy (SEM) and atomic force microscopy (AFM), UV-visible spectroscopy, and Fourier transform infrared spectroscopy (FTIR). Functional in vitro characterization relies on different methods for estimation of silver release, antimicrobial activity, and nanocomposite cytotoxicity. Here, we specially focus on utilization of 3D bioreactor systems that mimic native physiological environments with the aim to reliably predict nanocomposite behavior during implementation and so to decrease the need for animal experimentation. These systems were shown to provide more accurate and relevant data on silver release and cytotoxicity as compared to static systems such as 2D cell monolayer cultures. Finally, nanocomposites are evaluated in vivo in different animal models, which are in the case of wound dressings typically mice, rats, and pigs. The present review provides a basis for defining a strategy for comprehensive and efficient preclinical characterization of novel nanocomposites attractive not only for those containing AgNPs but also other metallic nanoparticles aimed for biomedical applications. Key points center dot A platform for devising comprehensive preclinical evaluation of nanocomposites. center dot Biomimetic bioreactors provide reliable functional nanocomposite evaluation. center dot Cells in 2D cultures are more sensitive to silver nanoparticles than in 3D cultures. center dot Biomimetic bioreactor 3D cell/tissue cultures can address the in vitro-in vivo gap.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Preclinical functional characterization methods of nanocomposite hydrogels containing silver nanoparticles for biomedical applications",
pages = "4658-4643",
number = "11",
volume = "104",
doi = "10.1007/s00253-020-10521-2"
}

Stojkovska, J., Zvicer, J.,& Obradović, B.. (2020). Preclinical functional characterization methods of nanocomposite hydrogels containing silver nanoparticles for biomedical applications. in Applied Microbiology and Biotechnology
Springer, New York., 104(11), 4643-4658.
https://doi.org/10.1007/s00253-020-10521-2

Stojkovska J, Zvicer J, Obradović B. Preclinical functional characterization methods of nanocomposite hydrogels containing silver nanoparticles for biomedical applications. in Applied Microbiology and Biotechnology. 2020;104(11):4643-4658.
doi:10.1007/s00253-020-10521-2 .

Stojkovska, Jasmina, Zvicer, Jovana, Obradović, Bojana, "Preclinical functional characterization methods of nanocomposite hydrogels containing silver nanoparticles for biomedical applications" in Applied Microbiology and Biotechnology, 104, no. 11 (2020):4643-4658,
https://doi.org/10.1007/s00253-020-10521-2 . .

TY  - JOUR
AU  - Zvicer, Jovana
AU  - Medić, Ana
AU  - Veljović, Đorđe
AU  - Jevtić, Sanja
AU  - Novak, Sasa
AU  - Obradović, Bojana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4051
AB  - The authors regret that Figures 4 and 5 in the above manuscript were exchanged. The authors would like to apologise for any inconvenience caused.
PB  - Elsevier Sci Ltd, Oxford
T2  - Polymer Testing
T1  - Biomimetic characterization reveals enhancement of hydroxyapatite formation by fluid flow in gellan gum and bioactive glass composite scaffolds (vol 76, pg 464, 2019)
SP  - 105908
VL  - 77
DO  - 10.1016/j.polymertesting.2019.105908
ER  - 

@article{
author = "Zvicer, Jovana and Medić, Ana and Veljović, Đorđe and Jevtić, Sanja and Novak, Sasa and Obradović, Bojana",
year = "2019",
abstract = "The authors regret that Figures 4 and 5 in the above manuscript were exchanged. The authors would like to apologise for any inconvenience caused.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Polymer Testing",
title = "Biomimetic characterization reveals enhancement of hydroxyapatite formation by fluid flow in gellan gum and bioactive glass composite scaffolds (vol 76, pg 464, 2019)",
pages = "105908",
volume = "77",
doi = "10.1016/j.polymertesting.2019.105908"
}

Zvicer, J., Medić, A., Veljović, Đ., Jevtić, S., Novak, S.,& Obradović, B.. (2019). Biomimetic characterization reveals enhancement of hydroxyapatite formation by fluid flow in gellan gum and bioactive glass composite scaffolds (vol 76, pg 464, 2019). in Polymer Testing
Elsevier Sci Ltd, Oxford., 77, 105908.
https://doi.org/10.1016/j.polymertesting.2019.105908

Zvicer J, Medić A, Veljović Đ, Jevtić S, Novak S, Obradović B. Biomimetic characterization reveals enhancement of hydroxyapatite formation by fluid flow in gellan gum and bioactive glass composite scaffolds (vol 76, pg 464, 2019). in Polymer Testing. 2019;77:105908.
doi:10.1016/j.polymertesting.2019.105908 .

Zvicer, Jovana, Medić, Ana, Veljović, Đorđe, Jevtić, Sanja, Novak, Sasa, Obradović, Bojana, "Biomimetic characterization reveals enhancement of hydroxyapatite formation by fluid flow in gellan gum and bioactive glass composite scaffolds (vol 76, pg 464, 2019)" in Polymer Testing, 77 (2019):105908,
https://doi.org/10.1016/j.polymertesting.2019.105908 . .

TY  - JOUR
AU  - Zvicer, Jovana
AU  - Medić, Ana
AU  - Veljović, Đorđe
AU  - Jevtić, Sanja
AU  - Novak, Sasa
AU  - Obradović, Bojana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4090
AB  - This study presents production and biomimetic characterization of macroporous composite scaffolds based on gellan gum and nanoparticulate bioactive glass (GG-BAG) under conditions relevant for bone tissue engineering. Formation of hydroxyapatite (HAp) within the scaffolds was investigated in the simulated body fluid (SBF) during 14 days in two biomimetic bioreactors: perfusion bioreactor (1.1 ml/min SBF flowrate) and a bioreactor with coupled dynamic compression and SBF perfusion (5% strain, 0.68 Hz, 1 h on/1 h off, 1.1 ml/min SBF flowrate). HAp formation was evaluated by scanning electron microscopy (FE-SEM), energy dispersive X-ray (EDS) analysis, Fourier transform infrared spectroscopy (FTIR), and X-ray powder diffraction (XRPD). The superficial SBF velocity of 100 mu m/s in perfusion bioreactors induced the formation of abundant cauliflower-like HAp crystals throughout the scaffold interior and flake-like crystals on external surfaces resulting in improved mechanical properties as compared to the initial scaffolds. The obtained results indicate potentials of macro-porous GG-BAG scaffolds in conjunction with perfusion bioreactors for bone tissue engineering demonstrating high bioactivity suited for cultures of osteogenic cells.
PB  - Elsevier Sci Ltd, Oxford
T2  - Polymer Testing
T1  - Biomimetic characterization reveals enhancement of hydroxyapatite formation by fluid flow in gellan gum and bioactive glass composite scaffolds
EP  - 472
SP  - 464
VL  - 76
DO  - 10.1016/j.polymertesting.2019.04.004
ER  - 

@article{
author = "Zvicer, Jovana and Medić, Ana and Veljović, Đorđe and Jevtić, Sanja and Novak, Sasa and Obradović, Bojana",
year = "2019",
abstract = "This study presents production and biomimetic characterization of macroporous composite scaffolds based on gellan gum and nanoparticulate bioactive glass (GG-BAG) under conditions relevant for bone tissue engineering. Formation of hydroxyapatite (HAp) within the scaffolds was investigated in the simulated body fluid (SBF) during 14 days in two biomimetic bioreactors: perfusion bioreactor (1.1 ml/min SBF flowrate) and a bioreactor with coupled dynamic compression and SBF perfusion (5% strain, 0.68 Hz, 1 h on/1 h off, 1.1 ml/min SBF flowrate). HAp formation was evaluated by scanning electron microscopy (FE-SEM), energy dispersive X-ray (EDS) analysis, Fourier transform infrared spectroscopy (FTIR), and X-ray powder diffraction (XRPD). The superficial SBF velocity of 100 mu m/s in perfusion bioreactors induced the formation of abundant cauliflower-like HAp crystals throughout the scaffold interior and flake-like crystals on external surfaces resulting in improved mechanical properties as compared to the initial scaffolds. The obtained results indicate potentials of macro-porous GG-BAG scaffolds in conjunction with perfusion bioreactors for bone tissue engineering demonstrating high bioactivity suited for cultures of osteogenic cells.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Polymer Testing",
title = "Biomimetic characterization reveals enhancement of hydroxyapatite formation by fluid flow in gellan gum and bioactive glass composite scaffolds",
pages = "472-464",
volume = "76",
doi = "10.1016/j.polymertesting.2019.04.004"
}

Zvicer, J., Medić, A., Veljović, Đ., Jevtić, S., Novak, S.,& Obradović, B.. (2019). Biomimetic characterization reveals enhancement of hydroxyapatite formation by fluid flow in gellan gum and bioactive glass composite scaffolds. in Polymer Testing
Elsevier Sci Ltd, Oxford., 76, 464-472.
https://doi.org/10.1016/j.polymertesting.2019.04.004

Zvicer J, Medić A, Veljović Đ, Jevtić S, Novak S, Obradović B. Biomimetic characterization reveals enhancement of hydroxyapatite formation by fluid flow in gellan gum and bioactive glass composite scaffolds. in Polymer Testing. 2019;76:464-472.
doi:10.1016/j.polymertesting.2019.04.004 .

Zvicer, Jovana, Medić, Ana, Veljović, Đorđe, Jevtić, Sanja, Novak, Sasa, Obradović, Bojana, "Biomimetic characterization reveals enhancement of hydroxyapatite formation by fluid flow in gellan gum and bioactive glass composite scaffolds" in Polymer Testing, 76 (2019):464-472,
https://doi.org/10.1016/j.polymertesting.2019.04.004 . .

TY  - JOUR
AU  - Zvicer, Jovana
AU  - Mišković-Stanković, Vesna
AU  - Obradović, Bojana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4191
AB  - In this work, functional characterization of biomaterials concerning potential application as articular cartilage implants was performed by using a biomimetic bioreactor with dynamic compression in the physiological regime (10% strain, 0.84 Hz frequency, 1 h on/1 h off). Specifically, two alginate types with low (LG) and high (HG) guluronic/mannuronic residue ratios with electrochemically synthesized silver nanoparticles (AgNPs) were evaluated. HG Ag/alginate hydrogels were clearly indicated as potential candidates due to better initial mechanical properties as compared to LG hydrogels (dynamic compression modulus of similar to 60 vs. similar to 40 kPa) as well as the mechanical stability displayed during 7 days of dynamic compression. Cytotoxicity studies in 3D bovine cartilage explant cultures under dynamic compression have shown negligible effects as compared to standard 2D monolayers of bovine chondrocytes where moderate cytotoxicity was observed. Finally, experimental and mathematical modeling studies revealed different mechanisms of AgNP release under physiological-like bioreactor conditions as compared to static conditions. Overall, the results clearly demonstrate bioreactor advantages in characterization and selection of candidate biomaterials as well as potentials to bridge the in vitro-in vivo gap.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants
EP  - 768
IS  - 4
SP  - 755
VL  - 107
DO  - 10.1002/jbm.a.36590
ER  - 

@article{
author = "Zvicer, Jovana and Mišković-Stanković, Vesna and Obradović, Bojana",
year = "2019",
abstract = "In this work, functional characterization of biomaterials concerning potential application as articular cartilage implants was performed by using a biomimetic bioreactor with dynamic compression in the physiological regime (10% strain, 0.84 Hz frequency, 1 h on/1 h off). Specifically, two alginate types with low (LG) and high (HG) guluronic/mannuronic residue ratios with electrochemically synthesized silver nanoparticles (AgNPs) were evaluated. HG Ag/alginate hydrogels were clearly indicated as potential candidates due to better initial mechanical properties as compared to LG hydrogels (dynamic compression modulus of similar to 60 vs. similar to 40 kPa) as well as the mechanical stability displayed during 7 days of dynamic compression. Cytotoxicity studies in 3D bovine cartilage explant cultures under dynamic compression have shown negligible effects as compared to standard 2D monolayers of bovine chondrocytes where moderate cytotoxicity was observed. Finally, experimental and mathematical modeling studies revealed different mechanisms of AgNP release under physiological-like bioreactor conditions as compared to static conditions. Overall, the results clearly demonstrate bioreactor advantages in characterization and selection of candidate biomaterials as well as potentials to bridge the in vitro-in vivo gap.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants",
pages = "768-755",
number = "4",
volume = "107",
doi = "10.1002/jbm.a.36590"
}

Zvicer, J., Mišković-Stanković, V.,& Obradović, B.. (2019). Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 107(4), 755-768.
https://doi.org/10.1002/jbm.a.36590

Zvicer J, Mišković-Stanković V, Obradović B. Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants. in Journal of Biomedical Materials Research Part A. 2019;107(4):755-768.
doi:10.1002/jbm.a.36590 .

Zvicer, Jovana, Mišković-Stanković, Vesna, Obradović, Bojana, "Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants" in Journal of Biomedical Materials Research Part A, 107, no. 4 (2019):755-768,
https://doi.org/10.1002/jbm.a.36590 . .

TY  - JOUR
AU  - Zvicer, Jovana
AU  - Mišković-Stanković, Vesna
AU  - Obradović, Bojana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5047
AB  - In this work, functional characterization of biomaterials concerning potential application as articular cartilage implants was performed by using a biomimetic bioreactor with dynamic compression in the physiological regime (10% strain, 0.84 Hz frequency, 1 h on/1 h off). Specifically, two alginate types with low (LG) and high (HG) guluronic/mannuronic residue ratios with electrochemically synthesized silver nanoparticles (AgNPs) were evaluated. HG Ag/alginate hydrogels were clearly indicated as potential candidates due to better initial mechanical properties as compared to LG hydrogels (dynamic compression modulus of ~60 vs. ~40 kPa) as well as the mechanical stability displayed during 7 days of dynamic compression. Cytotoxicity studies in 3D bovine cartilage explant cultures under dynamic compression have shown negligible effects as compared to standard 2D monolayers of bovine chondrocytes where moderate cytotoxicity was observed. Finally, experimental and mathematical modeling studies revealed different mechanisms of AgNP release under physiological-like bioreactor conditions as compared to static conditions. Overall, the results clearly demonstrate bioreactor advantages in characterization and selection of candidate biomaterials as well as potentials to bridge the in vitro-in vivo gap.
PB  - John Wiley and Sons Inc.
T2  - Journal of Biomedical Materials Research - Part A
T1  - Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants
EP  - 768
IS  - 4
SP  - 755
VL  - 107
DO  - 10.1002/jbm.a.36590
ER  - 

@article{
author = "Zvicer, Jovana and Mišković-Stanković, Vesna and Obradović, Bojana",
year = "2019",
abstract = "In this work, functional characterization of biomaterials concerning potential application as articular cartilage implants was performed by using a biomimetic bioreactor with dynamic compression in the physiological regime (10% strain, 0.84 Hz frequency, 1 h on/1 h off). Specifically, two alginate types with low (LG) and high (HG) guluronic/mannuronic residue ratios with electrochemically synthesized silver nanoparticles (AgNPs) were evaluated. HG Ag/alginate hydrogels were clearly indicated as potential candidates due to better initial mechanical properties as compared to LG hydrogels (dynamic compression modulus of ~60 vs. ~40 kPa) as well as the mechanical stability displayed during 7 days of dynamic compression. Cytotoxicity studies in 3D bovine cartilage explant cultures under dynamic compression have shown negligible effects as compared to standard 2D monolayers of bovine chondrocytes where moderate cytotoxicity was observed. Finally, experimental and mathematical modeling studies revealed different mechanisms of AgNP release under physiological-like bioreactor conditions as compared to static conditions. Overall, the results clearly demonstrate bioreactor advantages in characterization and selection of candidate biomaterials as well as potentials to bridge the in vitro-in vivo gap.",
publisher = "John Wiley and Sons Inc.",
journal = "Journal of Biomedical Materials Research - Part A",
title = "Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants",
pages = "768-755",
number = "4",
volume = "107",
doi = "10.1002/jbm.a.36590"
}

Zvicer, J., Mišković-Stanković, V.,& Obradović, B.. (2019). Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants. in Journal of Biomedical Materials Research - Part A
John Wiley and Sons Inc.., 107(4), 755-768.
https://doi.org/10.1002/jbm.a.36590

Zvicer J, Mišković-Stanković V, Obradović B. Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants. in Journal of Biomedical Materials Research - Part A. 2019;107(4):755-768.
doi:10.1002/jbm.a.36590 .

Zvicer, Jovana, Mišković-Stanković, Vesna, Obradović, Bojana, "Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants" in Journal of Biomedical Materials Research - Part A, 107, no. 4 (2019):755-768,
https://doi.org/10.1002/jbm.a.36590 . .

TY  - JOUR
AU  - Zvicer, Jovana
AU  - Obradović, Bojana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3952
AB  - Intervertebral discs are normally exposed to a variety of loads and stresses but hydrostatic pressure (HP) could be the main biosignal for chondrogenic cell differentiation and maintenance of this tissue. Although there are simple approaches to intermittently expose cell cultures to HP in separate material testing devices, utilization of biomimetic bioreactors aiming to provide in vitro conditions mimicking those found in vivo, attracts special attention. However, design of such bioreactors is complex due to the requirement of high HP magnitudes (up to 3MPa) applied in different regimes mimicking pressures arising in intervertebral disc during normal daily activities. Furthermore, efficient mass transfer has to be facilitated to cells within 3D scaffolds, and the engineering challenges include avoidance or removal of gas bubbles in the culture medium before pressurization as well as selection of appropriate, biocompatible construction materials and maintenance of sterility during cultivation. Here, we review approaches to induce HP in 2D and 3D cell cultures categorized into 5 groups: (I) discontinuous systems with direct pressurization of the cultivation medium by a piston, (II) discontinuous systems with indirect pressurization by a compression fluid, (III) continuous systems with direct pressurization of the cultivation medium, static culture, (IV) continuous systems with culture perfusion, and (V) systems applying HP in conjunction with other physical signals. Although the complexity is increasing as additional features are added to the systems, the need to understand HP effects on cells and tissues in a physiologically relevant, yet precisely controlled, environment together with current technological advancements are leading towards innovative bioreactor solutions.
PB  - Wiley, Hoboken
T2  - Journal of Tissue Engineering and Regenerative Medicine
T1  - Bioreactors with hydrostatic pressures imitating physiological environments in intervertebral discs
EP  - 545
IS  - 2
SP  - 529
VL  - 12
DO  - 10.1002/term.2533
ER  - 

@article{
author = "Zvicer, Jovana and Obradović, Bojana",
year = "2018",
abstract = "Intervertebral discs are normally exposed to a variety of loads and stresses but hydrostatic pressure (HP) could be the main biosignal for chondrogenic cell differentiation and maintenance of this tissue. Although there are simple approaches to intermittently expose cell cultures to HP in separate material testing devices, utilization of biomimetic bioreactors aiming to provide in vitro conditions mimicking those found in vivo, attracts special attention. However, design of such bioreactors is complex due to the requirement of high HP magnitudes (up to 3MPa) applied in different regimes mimicking pressures arising in intervertebral disc during normal daily activities. Furthermore, efficient mass transfer has to be facilitated to cells within 3D scaffolds, and the engineering challenges include avoidance or removal of gas bubbles in the culture medium before pressurization as well as selection of appropriate, biocompatible construction materials and maintenance of sterility during cultivation. Here, we review approaches to induce HP in 2D and 3D cell cultures categorized into 5 groups: (I) discontinuous systems with direct pressurization of the cultivation medium by a piston, (II) discontinuous systems with indirect pressurization by a compression fluid, (III) continuous systems with direct pressurization of the cultivation medium, static culture, (IV) continuous systems with culture perfusion, and (V) systems applying HP in conjunction with other physical signals. Although the complexity is increasing as additional features are added to the systems, the need to understand HP effects on cells and tissues in a physiologically relevant, yet precisely controlled, environment together with current technological advancements are leading towards innovative bioreactor solutions.",
publisher = "Wiley, Hoboken",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
title = "Bioreactors with hydrostatic pressures imitating physiological environments in intervertebral discs",
pages = "545-529",
number = "2",
volume = "12",
doi = "10.1002/term.2533"
}

Zvicer, J.,& Obradović, B.. (2018). Bioreactors with hydrostatic pressures imitating physiological environments in intervertebral discs. in Journal of Tissue Engineering and Regenerative Medicine
Wiley, Hoboken., 12(2), 529-545.
https://doi.org/10.1002/term.2533

Zvicer J, Obradović B. Bioreactors with hydrostatic pressures imitating physiological environments in intervertebral discs. in Journal of Tissue Engineering and Regenerative Medicine. 2018;12(2):529-545.
doi:10.1002/term.2533 .

Zvicer, Jovana, Obradović, Bojana, "Bioreactors with hydrostatic pressures imitating physiological environments in intervertebral discs" in Journal of Tissue Engineering and Regenerative Medicine, 12, no. 2 (2018):529-545,
https://doi.org/10.1002/term.2533 . .

TY  - CONF
AU  - Radonjić, Mia
AU  - Zvicer, Jovana
AU  - Obradović, Bojana
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6347
AB  - Scaffolds used for osteochondral tissue engineering should comprise two distinct regions: a
bottom region with characteristics corresponding to bone tissue, such as a porous structure
with mineral components (predominantly hydroxyapatite), and a top region with
characteristics of articular cartilage, which is gelatinous with high water content. In this
work, we have investigated possibilities to formulate and optimize a procedure for obtaining
such biphasic scaffolds based on gellan gum (GG). A porous base layer of the scaffold was
obtained by lyophilization of the 2 % GG hydrogel with dispersed bioactive glass
nanoparticles, as hydroxyapatite precursors. Next, different procedures were investigated to
produce the upper GG hydrogel such as partial immersion of the porous layer in the GG
solution and pouring the GG solution over the porous layer at different moisture conditions
and temperatures. A simple mathematical model was derived and subsequently
experimentally validated to find optimal temperatures of the porous layer, GG solution and
the surrounding environment to provide adequate gelation rate to form the GG hydrogel on
top of the porous layer with a thin interfacial zone.
PB  - Institute of Technical Sciences of SASA
C3  - Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
T1  - Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering
SP  - 4
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6347
ER  - 

@conference{
author = "Radonjić, Mia and Zvicer, Jovana and Obradović, Bojana",
year = "2017",
abstract = "Scaffolds used for osteochondral tissue engineering should comprise two distinct regions: a
bottom region with characteristics corresponding to bone tissue, such as a porous structure
with mineral components (predominantly hydroxyapatite), and a top region with
characteristics of articular cartilage, which is gelatinous with high water content. In this
work, we have investigated possibilities to formulate and optimize a procedure for obtaining
such biphasic scaffolds based on gellan gum (GG). A porous base layer of the scaffold was
obtained by lyophilization of the 2 % GG hydrogel with dispersed bioactive glass
nanoparticles, as hydroxyapatite precursors. Next, different procedures were investigated to
produce the upper GG hydrogel such as partial immersion of the porous layer in the GG
solution and pouring the GG solution over the porous layer at different moisture conditions
and temperatures. A simple mathematical model was derived and subsequently
experimentally validated to find optimal temperatures of the porous layer, GG solution and
the surrounding environment to provide adequate gelation rate to form the GG hydrogel on
top of the porous layer with a thin interfacial zone.",
publisher = "Institute of Technical Sciences of SASA",
journal = "Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts",
title = "Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering",
pages = "4",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6347"
}

Radonjić, M., Zvicer, J.,& Obradović, B.. (2017). Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering. in Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
Institute of Technical Sciences of SASA., 4.
https://hdl.handle.net/21.15107/rcub_technorep_6347

Radonjić M, Zvicer J, Obradović B. Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering. in Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts. 2017;:4.
https://hdl.handle.net/21.15107/rcub_technorep_6347 .

Radonjić, Mia, Zvicer, Jovana, Obradović, Bojana, "Development and optimization of the production procedure of biphasic scaffolds for osteochondral tissue engineering" in Seventieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts (2017):4,
https://hdl.handle.net/21.15107/rcub_technorep_6347 .

TY  - CONF
AU  - Radonjić, Mia
AU  - Zvicer, Jovana
AU  - Obradović, Bojana
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6349
PB  - Institute of Technical Sciences of the Serbian Academy of Sciences and Arts
C3  - 15th Young Researchers Conference – Materials Science and Engineering Programme and the Book of Abstracts
T1  - Operating conditions in the bioreactor prototype applying  hydrostatic pressures
SP  - 12
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6349
ER  - 

@conference{
author = "Radonjić, Mia and Zvicer, Jovana and Obradović, Bojana",
year = "2016",
publisher = "Institute of Technical Sciences of the Serbian Academy of Sciences and Arts",
journal = "15th Young Researchers Conference – Materials Science and Engineering Programme and the Book of Abstracts",
title = "Operating conditions in the bioreactor prototype applying  hydrostatic pressures",
pages = "12",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6349"
}

Radonjić, M., Zvicer, J.,& Obradović, B.. (2016). Operating conditions in the bioreactor prototype applying  hydrostatic pressures. in 15th Young Researchers Conference – Materials Science and Engineering Programme and the Book of Abstracts
Institute of Technical Sciences of the Serbian Academy of Sciences and Arts., 12.
https://hdl.handle.net/21.15107/rcub_technorep_6349

Radonjić M, Zvicer J, Obradović B. Operating conditions in the bioreactor prototype applying  hydrostatic pressures. in 15th Young Researchers Conference – Materials Science and Engineering Programme and the Book of Abstracts. 2016;:12.
https://hdl.handle.net/21.15107/rcub_technorep_6349 .

Radonjić, Mia, Zvicer, Jovana, Obradović, Bojana, "Operating conditions in the bioreactor prototype applying  hydrostatic pressures" in 15th Young Researchers Conference – Materials Science and Engineering Programme and the Book of Abstracts (2016):12,
https://hdl.handle.net/21.15107/rcub_technorep_6349 .

TY  - CONF
AU  - Zvicer, Jovana
AU  - Samardžić, M.
AU  - Mišković-Stanković, Vesna
AU  - Obradović, Bojana
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2880
AB  - Alginate nanocomposite hydrogels with incorporated electrochemically synthesized silver nanoparticles were investigated regarding cytotoxicity in vitro. Direct contact test of Ag/alginate discs was applied in 2D monolayer cultures of bovine calf chondrocytes while a 3D culture of bovine articular cartilage explants pressed by the discs was established in a biomimetic bioreactor with dynamic compression in the physiological regime (10 % strain, 0.84 Hz frequency, 1 h on / 1 h off). Moderate cytotoxicity was observed in 2D cell cultures as opposed to findings in 3D explant cultures, which were not affected by the Ag/alginate discs despite the compression.
PB  - 15th IEEE International Conference on Bioinformatics and Bioengineering, BIBE 2015
C3  - 2015 IEEE 15th International Conference on Bioinformatics and Bioengineering, BIBE 2015
T1  - Cytotoxicity studies of Ag/alginate nanocomposite hydrogels in 2D and 3D cultures
DO  - 10.1109/BIBE.2015.7367664
ER  - 

@conference{
author = "Zvicer, Jovana and Samardžić, M. and Mišković-Stanković, Vesna and Obradović, Bojana",
year = "2015",
abstract = "Alginate nanocomposite hydrogels with incorporated electrochemically synthesized silver nanoparticles were investigated regarding cytotoxicity in vitro. Direct contact test of Ag/alginate discs was applied in 2D monolayer cultures of bovine calf chondrocytes while a 3D culture of bovine articular cartilage explants pressed by the discs was established in a biomimetic bioreactor with dynamic compression in the physiological regime (10 % strain, 0.84 Hz frequency, 1 h on / 1 h off). Moderate cytotoxicity was observed in 2D cell cultures as opposed to findings in 3D explant cultures, which were not affected by the Ag/alginate discs despite the compression.",
publisher = "15th IEEE International Conference on Bioinformatics and Bioengineering, BIBE 2015",
journal = "2015 IEEE 15th International Conference on Bioinformatics and Bioengineering, BIBE 2015",
title = "Cytotoxicity studies of Ag/alginate nanocomposite hydrogels in 2D and 3D cultures",
doi = "10.1109/BIBE.2015.7367664"
}

Zvicer, J., Samardžić, M., Mišković-Stanković, V.,& Obradović, B.. (2015). Cytotoxicity studies of Ag/alginate nanocomposite hydrogels in 2D and 3D cultures. in 2015 IEEE 15th International Conference on Bioinformatics and Bioengineering, BIBE 2015
15th IEEE International Conference on Bioinformatics and Bioengineering, BIBE 2015..
https://doi.org/10.1109/BIBE.2015.7367664

Zvicer J, Samardžić M, Mišković-Stanković V, Obradović B. Cytotoxicity studies of Ag/alginate nanocomposite hydrogels in 2D and 3D cultures. in 2015 IEEE 15th International Conference on Bioinformatics and Bioengineering, BIBE 2015. 2015;.
doi:10.1109/BIBE.2015.7367664 .

Zvicer, Jovana, Samardžić, M., Mišković-Stanković, Vesna, Obradović, Bojana, "Cytotoxicity studies of Ag/alginate nanocomposite hydrogels in 2D and 3D cultures" in 2015 IEEE 15th International Conference on Bioinformatics and Bioengineering, BIBE 2015 (2015),
https://doi.org/10.1109/BIBE.2015.7367664 . .

TY  - JOUR
AU  - Zvicer, Jovana
AU  - Girandon, L.
AU  - Potocar, U.
AU  - Froehlich, M.
AU  - Jančić, Ivan
AU  - Bufan, Biljana
AU  - Milenković, Marina
AU  - Stojkovska, Jasmina
AU  - Mišković-Stanković, Vesna
AU  - Obradović, Bojana
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2827
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Tissue Engineering and Regenerative Medicine
T1  - Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment
EP  - 345
SP  - 345
VL  - 8
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2827
ER  - 

@article{
author = "Zvicer, Jovana and Girandon, L. and Potocar, U. and Froehlich, M. and Jančić, Ivan and Bufan, Biljana and Milenković, Marina and Stojkovska, Jasmina and Mišković-Stanković, Vesna and Obradović, Bojana",
year = "2014",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
title = "Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment",
pages = "345-345",
volume = "8",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2827"
}

Zvicer, J., Girandon, L., Potocar, U., Froehlich, M., Jančić, I., Bufan, B., Milenković, M., Stojkovska, J., Mišković-Stanković, V.,& Obradović, B.. (2014). Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment. in Journal of Tissue Engineering and Regenerative Medicine
Wiley-Blackwell, Hoboken., 8, 345-345.
https://hdl.handle.net/21.15107/rcub_technorep_2827

Zvicer J, Girandon L, Potocar U, Froehlich M, Jančić I, Bufan B, Milenković M, Stojkovska J, Mišković-Stanković V, Obradović B. Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment. in Journal of Tissue Engineering and Regenerative Medicine. 2014;8:345-345.
https://hdl.handle.net/21.15107/rcub_technorep_2827 .

Zvicer, Jovana, Girandon, L., Potocar, U., Froehlich, M., Jančić, Ivan, Bufan, Biljana, Milenković, Marina, Stojkovska, Jasmina, Mišković-Stanković, Vesna, Obradović, Bojana, "Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment" in Journal of Tissue Engineering and Regenerative Medicine, 8 (2014):345-345,
https://hdl.handle.net/21.15107/rcub_technorep_2827 .

TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Zvicer, Jovana
AU  - Jovanović, Željka
AU  - Mišković-Stanković, Vesna
AU  - Obradović, Bojana
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2078
AB  - The production of nanocomposite alginate microbeads with electrochemically synthesized silver nanoparticles (AgNPs) based on the electrostatic extrusion technique was investigated with respect to their potentials for utilization in pharmaceutical and biomedical applications. It was shown that electrochemical synthesis of AgNPs results in the reduction of practically all the Ag+ present in the initial solution, yielding stable Ag/alginate colloid solutions that were demonstrated to be suitable for sterilization, manipulation, and electrostatic extrusion with retention of the AgNPs. The presence of AgNPs in the alginate colloid solutions had negligible effects on the size of the produced Ag/alginate microbeads, which was mainly determined by the electrostatic potential applied during the extrusion. On the other hand, the incorporation of AgNPs within the alginate hydrogel induced slight changes in biomechanical properties of the microbeads determined in a biomimetic bioreactor. Thus, packed beds of nanocomposite Ag/alginate microbeads exhibited a slightly higher dynamic compression modulus as compared to that of control alginate microbeads (154±4 and 141±2 kPa, respectively). On the other hand, the equilibrium unconfined compression modulus was significantly lower for the nanocomposite microbeads as compared to that of the controls (34±2 and 47±1 kPa, respectively).
AB  - U ovom radu je ispitivan proces dobijanja elektrostatičkom ekstruzijom nanokompozitnih alginatnih mikročestica sa inkorporisanim elektrohemijski sintetisanim nanočesticama srebra u pogledu potencijala za primenu u farmaciji i biomedicini. Pokazalo se da tokom elektrohemijske sinteze nanočestica dolazi do praktično potpune redukcije Ag jona prisutnih u početnom rastvoru, dajući na taj način stabilne Ag/alginatne koloidne rastvore. Pokazano je zatim, da su ovi rastvori pogodni za sterilizaciju, manipulaciju, kao i elektrostatičku ekstruziju uz zadržavanje i očuvanje nanočestica. Prisustvo nanočestica srebra u koloidnim rastvorima nije imalo uticaj na veličinu dobijenih Ag/alginatnih mikročestica koja je pretežno bila određena vrednošću primenjenog elektrostatičkog napona tokom ekstruzije. Sa druge strane, inkorporacija nanočestica srebra unutar alginatnog hidrogela je uzrokovala male promene biomehaničkih karakteristika koje su određene u biomimičnom bioreaktoru. Naime, modul elastičnosti pakovanih slojeva nanokompozitnih Ag/alginatnih mikro-čestica određen pri dinamičkoj kompresiji je bio nešto veći nego kod pakovanog sloja kontrolnih alginatnih mikročestica (154±4 i 141±2 kPa, redom) dok je ravnotežni modul elastičnosti slojeva nanokompozitnih mikročestica bio značajno manji (34±2 i 47±1 kPa, redom).
PB  - Serbian Chemical Society, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Controlled production of alginate nanocomposites with incorporated silver nanoparticles aimed for biomedical applications
T1  - Kontrolisano dobijanje alginatnih nanokompozita sa inkorporisanim nanočesticama srebra u cilju biomedicinske primene
EP  - 1722
IS  - 12
SP  - 1709
VL  - 77
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2078
ER  - 

@article{
author = "Stojkovska, Jasmina and Zvicer, Jovana and Jovanović, Željka and Mišković-Stanković, Vesna and Obradović, Bojana",
year = "2012",
abstract = "The production of nanocomposite alginate microbeads with electrochemically synthesized silver nanoparticles (AgNPs) based on the electrostatic extrusion technique was investigated with respect to their potentials for utilization in pharmaceutical and biomedical applications. It was shown that electrochemical synthesis of AgNPs results in the reduction of practically all the Ag+ present in the initial solution, yielding stable Ag/alginate colloid solutions that were demonstrated to be suitable for sterilization, manipulation, and electrostatic extrusion with retention of the AgNPs. The presence of AgNPs in the alginate colloid solutions had negligible effects on the size of the produced Ag/alginate microbeads, which was mainly determined by the electrostatic potential applied during the extrusion. On the other hand, the incorporation of AgNPs within the alginate hydrogel induced slight changes in biomechanical properties of the microbeads determined in a biomimetic bioreactor. Thus, packed beds of nanocomposite Ag/alginate microbeads exhibited a slightly higher dynamic compression modulus as compared to that of control alginate microbeads (154±4 and 141±2 kPa, respectively). On the other hand, the equilibrium unconfined compression modulus was significantly lower for the nanocomposite microbeads as compared to that of the controls (34±2 and 47±1 kPa, respectively)., U ovom radu je ispitivan proces dobijanja elektrostatičkom ekstruzijom nanokompozitnih alginatnih mikročestica sa inkorporisanim elektrohemijski sintetisanim nanočesticama srebra u pogledu potencijala za primenu u farmaciji i biomedicini. Pokazalo se da tokom elektrohemijske sinteze nanočestica dolazi do praktično potpune redukcije Ag jona prisutnih u početnom rastvoru, dajući na taj način stabilne Ag/alginatne koloidne rastvore. Pokazano je zatim, da su ovi rastvori pogodni za sterilizaciju, manipulaciju, kao i elektrostatičku ekstruziju uz zadržavanje i očuvanje nanočestica. Prisustvo nanočestica srebra u koloidnim rastvorima nije imalo uticaj na veličinu dobijenih Ag/alginatnih mikročestica koja je pretežno bila određena vrednošću primenjenog elektrostatičkog napona tokom ekstruzije. Sa druge strane, inkorporacija nanočestica srebra unutar alginatnog hidrogela je uzrokovala male promene biomehaničkih karakteristika koje su određene u biomimičnom bioreaktoru. Naime, modul elastičnosti pakovanih slojeva nanokompozitnih Ag/alginatnih mikro-čestica određen pri dinamičkoj kompresiji je bio nešto veći nego kod pakovanog sloja kontrolnih alginatnih mikročestica (154±4 i 141±2 kPa, redom) dok je ravnotežni modul elastičnosti slojeva nanokompozitnih mikročestica bio značajno manji (34±2 i 47±1 kPa, redom).",
publisher = "Serbian Chemical Society, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Controlled production of alginate nanocomposites with incorporated silver nanoparticles aimed for biomedical applications, Kontrolisano dobijanje alginatnih nanokompozita sa inkorporisanim nanočesticama srebra u cilju biomedicinske primene",
pages = "1722-1709",
number = "12",
volume = "77",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2078"
}

Stojkovska, J., Zvicer, J., Jovanović, Ž., Mišković-Stanković, V.,& Obradović, B.. (2012). Controlled production of alginate nanocomposites with incorporated silver nanoparticles aimed for biomedical applications. in Journal of the Serbian Chemical Society
Serbian Chemical Society, Belgrade., 77(12), 1709-1722.
https://hdl.handle.net/21.15107/rcub_technorep_2078

Stojkovska J, Zvicer J, Jovanović Ž, Mišković-Stanković V, Obradović B. Controlled production of alginate nanocomposites with incorporated silver nanoparticles aimed for biomedical applications. in Journal of the Serbian Chemical Society. 2012;77(12):1709-1722.
https://hdl.handle.net/21.15107/rcub_technorep_2078 .

Stojkovska, Jasmina, Zvicer, Jovana, Jovanović, Željka, Mišković-Stanković, Vesna, Obradović, Bojana, "Controlled production of alginate nanocomposites with incorporated silver nanoparticles aimed for biomedical applications" in Journal of the Serbian Chemical Society, 77, no. 12 (2012):1709-1722,
https://hdl.handle.net/21.15107/rcub_technorep_2078 .

TY  - JOUR
AU  - Vidović, Srđan
AU  - Zvicer, Jovana
AU  - Stojkovska, Jasmina
AU  - Mišković-Stanković, Vesna
AU  - Obradović, Bojana
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2119
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Tissue Engineering and Regenerative Medicine
T1  - Nanocomposite microfibers based on alginate and PVA hydrogels with incorporated silver nanoparticles
EP  - 189
SP  - 189
VL  - 6
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2119
ER  - 

@article{
author = "Vidović, Srđan and Zvicer, Jovana and Stojkovska, Jasmina and Mišković-Stanković, Vesna and Obradović, Bojana",
year = "2012",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
title = "Nanocomposite microfibers based on alginate and PVA hydrogels with incorporated silver nanoparticles",
pages = "189-189",
volume = "6",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2119"
}

Vidović, S., Zvicer, J., Stojkovska, J., Mišković-Stanković, V.,& Obradović, B.. (2012). Nanocomposite microfibers based on alginate and PVA hydrogels with incorporated silver nanoparticles. in Journal of Tissue Engineering and Regenerative Medicine
Wiley-Blackwell, Hoboken., 6, 189-189.
https://hdl.handle.net/21.15107/rcub_technorep_2119

Vidović S, Zvicer J, Stojkovska J, Mišković-Stanković V, Obradović B. Nanocomposite microfibers based on alginate and PVA hydrogels with incorporated silver nanoparticles. in Journal of Tissue Engineering and Regenerative Medicine. 2012;6:189-189.
https://hdl.handle.net/21.15107/rcub_technorep_2119 .

Vidović, Srđan, Zvicer, Jovana, Stojkovska, Jasmina, Mišković-Stanković, Vesna, Obradović, Bojana, "Nanocomposite microfibers based on alginate and PVA hydrogels with incorporated silver nanoparticles" in Journal of Tissue Engineering and Regenerative Medicine, 6 (2012):189-189,
https://hdl.handle.net/21.15107/rcub_technorep_2119 .

TY  - CONF
AU  - Stojkovska, Jasmina
AU  - Zvicer, Jovana
AU  - Kostić, Danijela
AU  - Obradović, Bojana
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1773
PB  - 24th European Conference on Biomaterials, EBS 2011
C3  - 24th European Conference on Biomaterials - Annual Conference of the European Society for Biomaterial
T1  - Biomechanical properties of alginate hydrogels in a biomimetic bioreactor for cartilage tissue engineering
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1773
ER  - 

@conference{
author = "Stojkovska, Jasmina and Zvicer, Jovana and Kostić, Danijela and Obradović, Bojana",
year = "2011",
publisher = "24th European Conference on Biomaterials, EBS 2011",
journal = "24th European Conference on Biomaterials - Annual Conference of the European Society for Biomaterial",
title = "Biomechanical properties of alginate hydrogels in a biomimetic bioreactor for cartilage tissue engineering",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1773"
}

Stojkovska, J., Zvicer, J., Kostić, D.,& Obradović, B.. (2011). Biomechanical properties of alginate hydrogels in a biomimetic bioreactor for cartilage tissue engineering. in 24th European Conference on Biomaterials - Annual Conference of the European Society for Biomaterial
24th European Conference on Biomaterials, EBS 2011..
https://hdl.handle.net/21.15107/rcub_technorep_1773

Stojkovska J, Zvicer J, Kostić D, Obradović B. Biomechanical properties of alginate hydrogels in a biomimetic bioreactor for cartilage tissue engineering. in 24th European Conference on Biomaterials - Annual Conference of the European Society for Biomaterial. 2011;.
https://hdl.handle.net/21.15107/rcub_technorep_1773 .

Stojkovska, Jasmina, Zvicer, Jovana, Kostić, Danijela, Obradović, Bojana, "Biomechanical properties of alginate hydrogels in a biomimetic bioreactor for cartilage tissue engineering" in 24th European Conference on Biomaterials - Annual Conference of the European Society for Biomaterial (2011),
https://hdl.handle.net/21.15107/rcub_technorep_1773 .