TY  - JOUR
AU  - Đukić, Tamara
AU  - Drvenica, Ivana
AU  - Kovačić, Marijana
AU  - Minić, Rajna
AU  - Vučetić, Dušan
AU  - Majerič, Dragana
AU  - Šefik-Bukilica, Mirjana
AU  - Savić, Olivera
AU  - Bugarski, Branko
AU  - Ilić, Vesna
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6470
AB  - The size of circulating immune complexes (CICs) in rheumatoid arthritis (RA) could be an emerging criterion in disease diagnosis. This study analyzed size and electrokinetic potential of CICs from RA patients, healthy young adults, and RA patients age-matched controls aiming to establish their unique CIC features. Pooled CIC of 30 RA patients, 30 young adults, and 30 RA group's age-matched controls (middle-aged and oldеr healthy adults), and in vitro IgG aggregates from pooled sera of 300 healthy volunteers were tested using dynamic light scattering (DLS). Size distribution of CIC in healthy young adults exhibited high polydispersity. RA CIC patients and their age-matched control showed distinctly narrower size distributions compared with young adults. In these groups, particles clustered around two well-defined peaks. Particles of peak 1 were 36.1 ± 6.8 nm in RA age-matched control, and 30.8 ± 4.2 nm in RA patients. Particles of peak 2 of the RA age-matched control's CIC was 251.7 ± 41.2 nm, while RA CIC contained larger particles (359.9 ± 50.5 nm). The lower zeta potential of RA CIC, compared to control, indicated a disease-related decrease in colloidal stability. DLS identified RA-specific, but also age-specific distribution of CIC size and opened possibility of becoming a method for CIC size analysis in IC-mediated diseases.
PB  - Academic Press Inc.
T2  - Analytical Biochemistry
T1  - Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients
SP  - 115194
VL  - 674
DO  - 10.1016/j.ab.2023.115194
ER  - 

@article{
author = "Đukić, Tamara and Drvenica, Ivana and Kovačić, Marijana and Minić, Rajna and Vučetić, Dušan and Majerič, Dragana and Šefik-Bukilica, Mirjana and Savić, Olivera and Bugarski, Branko and Ilić, Vesna",
year = "2023",
abstract = "The size of circulating immune complexes (CICs) in rheumatoid arthritis (RA) could be an emerging criterion in disease diagnosis. This study analyzed size and electrokinetic potential of CICs from RA patients, healthy young adults, and RA patients age-matched controls aiming to establish their unique CIC features. Pooled CIC of 30 RA patients, 30 young adults, and 30 RA group's age-matched controls (middle-aged and oldеr healthy adults), and in vitro IgG aggregates from pooled sera of 300 healthy volunteers were tested using dynamic light scattering (DLS). Size distribution of CIC in healthy young adults exhibited high polydispersity. RA CIC patients and their age-matched control showed distinctly narrower size distributions compared with young adults. In these groups, particles clustered around two well-defined peaks. Particles of peak 1 were 36.1 ± 6.8 nm in RA age-matched control, and 30.8 ± 4.2 nm in RA patients. Particles of peak 2 of the RA age-matched control's CIC was 251.7 ± 41.2 nm, while RA CIC contained larger particles (359.9 ± 50.5 nm). The lower zeta potential of RA CIC, compared to control, indicated a disease-related decrease in colloidal stability. DLS identified RA-specific, but also age-specific distribution of CIC size and opened possibility of becoming a method for CIC size analysis in IC-mediated diseases.",
publisher = "Academic Press Inc.",
journal = "Analytical Biochemistry",
title = "Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients",
pages = "115194",
volume = "674",
doi = "10.1016/j.ab.2023.115194"
}

Đukić, T., Drvenica, I., Kovačić, M., Minić, R., Vučetić, D., Majerič, D., Šefik-Bukilica, M., Savić, O., Bugarski, B.,& Ilić, V.. (2023). Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients. in Analytical Biochemistry
Academic Press Inc.., 674, 115194.
https://doi.org/10.1016/j.ab.2023.115194

Đukić T, Drvenica I, Kovačić M, Minić R, Vučetić D, Majerič D, Šefik-Bukilica M, Savić O, Bugarski B, Ilić V. Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients. in Analytical Biochemistry. 2023;674:115194.
doi:10.1016/j.ab.2023.115194 .

Đukić, Tamara, Drvenica, Ivana, Kovačić, Marijana, Minić, Rajna, Vučetić, Dušan, Majerič, Dragana, Šefik-Bukilica, Mirjana, Savić, Olivera, Bugarski, Branko, Ilić, Vesna, "Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients" in Analytical Biochemistry, 674 (2023):115194,
https://doi.org/10.1016/j.ab.2023.115194 . .

TY  - JOUR
AU  - Stančić, Ana Z.
AU  - Drvenica, Ivana T.
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Branko M.
AU  - Bugarski, Diana S.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5339
AB  - Exploring the potential usage of the acellular preparation of porcine hemoglobin (PHb) isolated from slaughterhouse blood as a cell culture media component, we have tested its effects on the functional characteristics of stromal cells of mesodermal origin. Human peripheral blood mesenchymal stromal cells (PB-MSCs) were used in this study as a primary cell model system, along with three mouse cell lines (ATDC5, MC3T3-E1, and 3T3-L1), which represent more uniform model systems. We investigated the effect of PHb at concentrations of 0.1, 1, and 10 μM on these cells’ proliferation, cycle, and clonogenic and migratory potential, and found that PHb’s effect depended on both the cell type and its concentration. At the lowest concentration used (0.1 μM), PHb showed the least evident impact on the cell growth and migration; hence, we analyzed its effect on mesenchymal cell multilineage differentiation capacity at this concentration. Even under conditions that induce a specific type of MSC differentiation (cultivation in particular differentiation media), PHb modulated chondrogenic, osteogenic, and adipogenic differentiation, making it a potential candidate for a supplement of MSC culture. Through a model of porcine hemoglobin, these findings also contribute to improving the knowledge of extracellular hemoglobin’s influence on MSCs in vivo.
PB  - MDPI
T2  - Processes
T1  - Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin
IS  - 1
SP  - 32
VL  - 10
DO  - 10.3390/pr10010032
ER  - 

@article{
author = "Stančić, Ana Z. and Drvenica, Ivana T. and Ilić, Vesna Lj. and Bugarski, Branko M. and Bugarski, Diana S.",
year = "2022",
abstract = "Exploring the potential usage of the acellular preparation of porcine hemoglobin (PHb) isolated from slaughterhouse blood as a cell culture media component, we have tested its effects on the functional characteristics of stromal cells of mesodermal origin. Human peripheral blood mesenchymal stromal cells (PB-MSCs) were used in this study as a primary cell model system, along with three mouse cell lines (ATDC5, MC3T3-E1, and 3T3-L1), which represent more uniform model systems. We investigated the effect of PHb at concentrations of 0.1, 1, and 10 μM on these cells’ proliferation, cycle, and clonogenic and migratory potential, and found that PHb’s effect depended on both the cell type and its concentration. At the lowest concentration used (0.1 μM), PHb showed the least evident impact on the cell growth and migration; hence, we analyzed its effect on mesenchymal cell multilineage differentiation capacity at this concentration. Even under conditions that induce a specific type of MSC differentiation (cultivation in particular differentiation media), PHb modulated chondrogenic, osteogenic, and adipogenic differentiation, making it a potential candidate for a supplement of MSC culture. Through a model of porcine hemoglobin, these findings also contribute to improving the knowledge of extracellular hemoglobin’s influence on MSCs in vivo.",
publisher = "MDPI",
journal = "Processes",
title = "Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin",
number = "1",
pages = "32",
volume = "10",
doi = "10.3390/pr10010032"
}

Stančić, A. Z., Drvenica, I. T., Ilić, V. Lj., Bugarski, B. M.,& Bugarski, D. S.. (2022). Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin. in Processes
MDPI., 10(1), 32.
https://doi.org/10.3390/pr10010032

Stančić AZ, Drvenica IT, Ilić VL, Bugarski BM, Bugarski DS. Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin. in Processes. 2022;10(1):32.
doi:10.3390/pr10010032 .

Stančić, Ana Z., Drvenica, Ivana T., Ilić, Vesna Lj., Bugarski, Branko M., Bugarski, Diana S., "Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin" in Processes, 10, no. 1 (2022):32,
https://doi.org/10.3390/pr10010032 . .

TY  - JOUR
AU  - Nikodijević, Slavomir
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Kosanović, Dejana
AU  - Đukić, Tamara
AU  - Djordjević, Brižita
AU  - Ilić, Vesna
AU  - Minić, Rajna
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5235
AB  - Increased interest in microbiota calls for the thorough analysis of antibody reactivity to different microorganisms. As salivary IgA represents the first line of defence against microorganisms contacting mucosal surfaces, we explored the binding and specificity of salivary IgA by testing the binding of purified, FITC-labelled salivary IgA to different microorganisms in flow cytometry and conclude that this kind of analysis enables the differentiation of species/strains with high IgA binding capacity, which should be corroborated on a larger sample size. Further we compare, with in-house ELISA, the binding of polyclonal salivary IgA with the binding of polyclonal serum IgA from the same individuals to whole microbial cells and to purified microbial components. High correlations were obtained in total salivary IgA binding to Lactobacillus rhamnosus and Escherichia coli, very distant bacterial species, as well as to isolated bacterial components (r =.70–.97). The binding of total salivary IgA resembled the binding of both salivary IgA1 and IgA2, with IgA2 predominating. For serum polyclonal IgA repertoire, substantially higher specificity was obtained. Serum IgA binding to E. coli correlated best with serum IgA binding to lipopolysaccharide (r =.86), and serum IgA against L. rhamnosus correlated best with the anti-peptidoglycan IgA levels (r =.88). We have also detected that total serum IgA response is governed by either IgA1 or IgA2 response, depending on the nature of the antigen/s. We conclude that steady state salivary IgA repertoire, unlike serum IgA repertoire, consists of polyreactive antibodies with innate specificity, questioning its capacity to select resident microbiota.
PB  - John Wiley and Sons Inc
T2  - Scandinavian Journal of Immunology
T1  - Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans
DO  - 10.1111/sji.13223
ER  - 

@article{
author = "Nikodijević, Slavomir and Blagojević, Veljko and Ćuruvija, Ivana and Kosanović, Dejana and Đukić, Tamara and Djordjević, Brižita and Ilić, Vesna and Minić, Rajna",
year = "2022",
abstract = "Increased interest in microbiota calls for the thorough analysis of antibody reactivity to different microorganisms. As salivary IgA represents the first line of defence against microorganisms contacting mucosal surfaces, we explored the binding and specificity of salivary IgA by testing the binding of purified, FITC-labelled salivary IgA to different microorganisms in flow cytometry and conclude that this kind of analysis enables the differentiation of species/strains with high IgA binding capacity, which should be corroborated on a larger sample size. Further we compare, with in-house ELISA, the binding of polyclonal salivary IgA with the binding of polyclonal serum IgA from the same individuals to whole microbial cells and to purified microbial components. High correlations were obtained in total salivary IgA binding to Lactobacillus rhamnosus and Escherichia coli, very distant bacterial species, as well as to isolated bacterial components (r =.70–.97). The binding of total salivary IgA resembled the binding of both salivary IgA1 and IgA2, with IgA2 predominating. For serum polyclonal IgA repertoire, substantially higher specificity was obtained. Serum IgA binding to E. coli correlated best with serum IgA binding to lipopolysaccharide (r =.86), and serum IgA against L. rhamnosus correlated best with the anti-peptidoglycan IgA levels (r =.88). We have also detected that total serum IgA response is governed by either IgA1 or IgA2 response, depending on the nature of the antigen/s. We conclude that steady state salivary IgA repertoire, unlike serum IgA repertoire, consists of polyreactive antibodies with innate specificity, questioning its capacity to select resident microbiota.",
publisher = "John Wiley and Sons Inc",
journal = "Scandinavian Journal of Immunology",
title = "Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans",
doi = "10.1111/sji.13223"
}

Nikodijević, S., Blagojević, V., Ćuruvija, I., Kosanović, D., Đukić, T., Djordjević, B., Ilić, V.,& Minić, R.. (2022). Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans. in Scandinavian Journal of Immunology
John Wiley and Sons Inc..
https://doi.org/10.1111/sji.13223

Nikodijević S, Blagojević V, Ćuruvija I, Kosanović D, Đukić T, Djordjević B, Ilić V, Minić R. Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans. in Scandinavian Journal of Immunology. 2022;.
doi:10.1111/sji.13223 .

Nikodijević, Slavomir, Blagojević, Veljko, Ćuruvija, Ivana, Kosanović, Dejana, Đukić, Tamara, Djordjević, Brižita, Ilić, Vesna, Minić, Rajna, "Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans" in Scandinavian Journal of Immunology (2022),
https://doi.org/10.1111/sji.13223 . .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Bugarski, Branko
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Diana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4441
AB  - Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of SCs; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the experimental model for extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 mu M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1 alpha) at a concentration of 10 mu M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation
EP  - 391
IS  - 3
SP  - 379
VL  - 72
DO  - 10.2298/ABS200625032S
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Bugarski, Branko and Ilić, Vesna Lj. and Bugarski, Diana",
year = "2020",
abstract = "Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of SCs; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the experimental model for extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 mu M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1 alpha) at a concentration of 10 mu M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation",
pages = "391-379",
number = "3",
volume = "72",
doi = "10.2298/ABS200625032S"
}

Stančić, A., Drvenica, I., Bugarski, B., Ilić, V. Lj.,& Bugarski, D.. (2020). Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 72(3), 379-391.
https://doi.org/10.2298/ABS200625032S

Stančić A, Drvenica I, Bugarski B, Ilić VL, Bugarski D. Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation. in Archives of Biological Sciences. 2020;72(3):379-391.
doi:10.2298/ABS200625032S .

Stančić, Ana, Drvenica, Ivana, Bugarski, Branko, Ilić, Vesna Lj., Bugarski, Diana, "Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation" in Archives of Biological Sciences, 72, no. 3 (2020):379-391,
https://doi.org/10.2298/ABS200625032S . .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Obradović, Hristina
AU  - Bugarski, Branko
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Diana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4483
AB  - We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro
EP  - 920
SP  - 909
VL  - 144
DO  - 10.1016/j.ijbiomac.2019.09.167
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Obradović, Hristina and Bugarski, Branko and Ilić, Vesna Lj. and Bugarski, Diana",
year = "2020",
abstract = "We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro",
pages = "920-909",
volume = "144",
doi = "10.1016/j.ijbiomac.2019.09.167"
}

Stančić, A., Drvenica, I., Obradović, H., Bugarski, B., Ilić, V. Lj.,& Bugarski, D.. (2020). Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 144, 909-920.
https://doi.org/10.1016/j.ijbiomac.2019.09.167

Stančić A, Drvenica I, Obradović H, Bugarski B, Ilić VL, Bugarski D. Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules. 2020;144:909-920.
doi:10.1016/j.ijbiomac.2019.09.167 .

Stančić, Ana, Drvenica, Ivana, Obradović, Hristina, Bugarski, Branko, Ilić, Vesna Lj., Bugarski, Diana, "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro" in International Journal of Biological Macromolecules, 144 (2020):909-920,
https://doi.org/10.1016/j.ijbiomac.2019.09.167 . .

TY  - CHAP
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Bugarski, Branko
AU  - Pajić-Lijaković, Ivana
AU  - Maslovarić, I.
AU  - Ilić, Vesna Lj.
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4194
AB  - For many decades, the red blood cell membranes were in focus of research interest solely as a model system for investigation into the various membrane-related phenomena, composition/organization or membrane transport properties, as well as the comparative proteomic and lipidomic analyses in health and disease. During 50s, along with the first experimental steps in ATP encapsulation in erythrocytes membranes, these entities begin to fascinate clinicians and researchers by their super carrier capabilities for the controlled and targeted delivery (vascular, pulmonary, subcutaneous) of wide range of conventional drugs and biologicals. A relatively new realm for erythrocyte membrane is its application in targeted delivery of nanoparticles, like erythrocyte membrane cloaked nanoparticles, incorporating their most useful traits such as long circulation and stealth features. This chapter focuses on red blood cell membrane as unique constituent of drug delivery systems, including nano-sized ones (nanoerythrosomes) and ex vivo technologies for their preparation. Rheological characterization of membranes as well as the change induced by various experimental conditions is prerequisite for their application as drug carriers. The membrane viscoelasticity described by appropriate constitutive model is related to kinetic of drug release in order to whole process optimization. Furthermore, chapter will bring review of developed hybrid drug delivery vehicles of erythrocyte membranes as natural bio-derivative material, and nanoparticles, mainly made of synthetic material, whose combined advantages serve as immunologically non-invasive drug delivery platform. The advantages and drawbacks are specifically summarized to get critical point of view on existing and future medical applications of erythrocyte membrane as drug carriers. As an example of complexity in research toward development of such erythrocyte membrane based drug delivery systems starting from animal erythrocyte, morphological, biochemical and drug release profile assessment will be reviewed.
T2  - Erythrocytes: Structure, Functions and Clinical Aspects
T1  - Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems
EP  - 132
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4194
ER  - 

@inbook{
author = "Drvenica, Ivana and Stančić, Ana and Bugarski, Branko and Pajić-Lijaković, Ivana and Maslovarić, I. and Ilić, Vesna Lj.",
year = "2019",
abstract = "For many decades, the red blood cell membranes were in focus of research interest solely as a model system for investigation into the various membrane-related phenomena, composition/organization or membrane transport properties, as well as the comparative proteomic and lipidomic analyses in health and disease. During 50s, along with the first experimental steps in ATP encapsulation in erythrocytes membranes, these entities begin to fascinate clinicians and researchers by their super carrier capabilities for the controlled and targeted delivery (vascular, pulmonary, subcutaneous) of wide range of conventional drugs and biologicals. A relatively new realm for erythrocyte membrane is its application in targeted delivery of nanoparticles, like erythrocyte membrane cloaked nanoparticles, incorporating their most useful traits such as long circulation and stealth features. This chapter focuses on red blood cell membrane as unique constituent of drug delivery systems, including nano-sized ones (nanoerythrosomes) and ex vivo technologies for their preparation. Rheological characterization of membranes as well as the change induced by various experimental conditions is prerequisite for their application as drug carriers. The membrane viscoelasticity described by appropriate constitutive model is related to kinetic of drug release in order to whole process optimization. Furthermore, chapter will bring review of developed hybrid drug delivery vehicles of erythrocyte membranes as natural bio-derivative material, and nanoparticles, mainly made of synthetic material, whose combined advantages serve as immunologically non-invasive drug delivery platform. The advantages and drawbacks are specifically summarized to get critical point of view on existing and future medical applications of erythrocyte membrane as drug carriers. As an example of complexity in research toward development of such erythrocyte membrane based drug delivery systems starting from animal erythrocyte, morphological, biochemical and drug release profile assessment will be reviewed.",
journal = "Erythrocytes: Structure, Functions and Clinical Aspects",
booktitle = "Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems",
pages = "132-57",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4194"
}

Drvenica, I., Stančić, A., Bugarski, B., Pajić-Lijaković, I., Maslovarić, I.,& Ilić, V. Lj.. (2019). Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems. in Erythrocytes: Structure, Functions and Clinical Aspects, 57-132.
https://hdl.handle.net/21.15107/rcub_technorep_4194

Drvenica I, Stančić A, Bugarski B, Pajić-Lijaković I, Maslovarić I, Ilić VL. Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems. in Erythrocytes: Structure, Functions and Clinical Aspects. 2019;:57-132.
https://hdl.handle.net/21.15107/rcub_technorep_4194 .

Drvenica, Ivana, Stančić, Ana, Bugarski, Branko, Pajić-Lijaković, Ivana, Maslovarić, I., Ilić, Vesna Lj., "Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems" in Erythrocytes: Structure, Functions and Clinical Aspects (2019):57-132,
https://hdl.handle.net/21.15107/rcub_technorep_4194 .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Kalušević, Ana
AU  - Marković, Smilja
AU  - Dragišić-Maksimović, Jelena
AU  - Nedović, Viktor
AU  - Bugarski, Branko
AU  - Ilić, Vesna Lj.
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4135
AB  - Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray-and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20 +/- 5 degrees C) in solid forms, protected from moisture and light, rehydrated spray- and freeze- dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze- dried HbM, but their agglomerates in Hb samples. UV-Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin
EP  - 1117
IS  - 10
SP  - 1105
VL  - 84
DO  - 10.2298/JSC190513067D
ER  - 

@article{
author = "Drvenica, Ivana and Stančić, Ana and Kalušević, Ana and Marković, Smilja and Dragišić-Maksimović, Jelena and Nedović, Viktor and Bugarski, Branko and Ilić, Vesna Lj.",
year = "2019",
abstract = "Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray-and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20 +/- 5 degrees C) in solid forms, protected from moisture and light, rehydrated spray- and freeze- dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze- dried HbM, but their agglomerates in Hb samples. UV-Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin",
pages = "1117-1105",
number = "10",
volume = "84",
doi = "10.2298/JSC190513067D"
}

Drvenica, I., Stančić, A., Kalušević, A., Marković, S., Dragišić-Maksimović, J., Nedović, V., Bugarski, B.,& Ilić, V. Lj.. (2019). Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(10), 1105-1117.
https://doi.org/10.2298/JSC190513067D

Drvenica I, Stančić A, Kalušević A, Marković S, Dragišić-Maksimović J, Nedović V, Bugarski B, Ilić VL. Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin. in Journal of the Serbian Chemical Society. 2019;84(10):1105-1117.
doi:10.2298/JSC190513067D .

Drvenica, Ivana, Stančić, Ana, Kalušević, Ana, Marković, Smilja, Dragišić-Maksimović, Jelena, Nedović, Viktor, Bugarski, Branko, Ilić, Vesna Lj., "Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin" in Journal of the Serbian Chemical Society, 84, no. 10 (2019):1105-1117,
https://doi.org/10.2298/JSC190513067D . .

TY  - JOUR
AU  - Bukara, Katarina
AU  - Jovanić, Svetlana
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Ilić, Vesna Lj.
AU  - Rabasović, Mihailo D.
AU  - Pantelić, Dejan
AU  - Jelenković, Branislav
AU  - Bugarski, Branko
AU  - Krmpot, Aleksandar
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3747
AB  - The present study describes utilization of two photon excitation fluorescence (2PE) microscopy for visualization of the hemoglobin in human and porcine erythrocytes and their empty membranes (i.e., ghosts). High-quality, label-and fixation-free visualization of hemoglobin was achieved at excitation wavelength 730 nm by detecting visible autofluorescence. Localization in the suspension and spatial distribution (i.e., mapping) of residual hemoglobin in erythrocyte ghosts has been resolved by 2PE. Prior to the 2PE mapping, the presence of residual hemoglobin in the bulk suspension of erythrocyte ghosts was confirmed by cyanmethemoglobin assay. 2PE analysis revealed that the distribution of hemoglobin in intact erythrocytes follows the cells' shape. Two types of erythrocytes, human and porcine, characterized with discocyte and echinocyte morphology, respectively, showed significant differences in hemoglobin distribution. The 2PE images have revealed that despite an extensive washing out procedure after gradual hypotonic hemolysis, a certain amount of hemoglobin localized on the intracellular side always remains bound to the membrane and cannot be eliminated. The obtained results open the possibility to use 2PE microscopy to examine hemoglobin distribution in erythrocytes and estimate the purity level of erythrocyte ghosts in biotechnological processes.
PB  - Spie-Soc Photo-Optical Instrumentation Engineers, Bellingham
T2  - Journal of Biomedical Optics
T1  - Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy
IS  - 2
VL  - 22
DO  - 10.1117/1.JBO.22.2.026003
ER  - 

@article{
author = "Bukara, Katarina and Jovanić, Svetlana and Drvenica, Ivana and Stančić, Ana and Ilić, Vesna Lj. and Rabasović, Mihailo D. and Pantelić, Dejan and Jelenković, Branislav and Bugarski, Branko and Krmpot, Aleksandar",
year = "2017",
abstract = "The present study describes utilization of two photon excitation fluorescence (2PE) microscopy for visualization of the hemoglobin in human and porcine erythrocytes and their empty membranes (i.e., ghosts). High-quality, label-and fixation-free visualization of hemoglobin was achieved at excitation wavelength 730 nm by detecting visible autofluorescence. Localization in the suspension and spatial distribution (i.e., mapping) of residual hemoglobin in erythrocyte ghosts has been resolved by 2PE. Prior to the 2PE mapping, the presence of residual hemoglobin in the bulk suspension of erythrocyte ghosts was confirmed by cyanmethemoglobin assay. 2PE analysis revealed that the distribution of hemoglobin in intact erythrocytes follows the cells' shape. Two types of erythrocytes, human and porcine, characterized with discocyte and echinocyte morphology, respectively, showed significant differences in hemoglobin distribution. The 2PE images have revealed that despite an extensive washing out procedure after gradual hypotonic hemolysis, a certain amount of hemoglobin localized on the intracellular side always remains bound to the membrane and cannot be eliminated. The obtained results open the possibility to use 2PE microscopy to examine hemoglobin distribution in erythrocytes and estimate the purity level of erythrocyte ghosts in biotechnological processes.",
publisher = "Spie-Soc Photo-Optical Instrumentation Engineers, Bellingham",
journal = "Journal of Biomedical Optics",
title = "Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy",
number = "2",
volume = "22",
doi = "10.1117/1.JBO.22.2.026003"
}

Bukara, K., Jovanić, S., Drvenica, I., Stančić, A., Ilić, V. Lj., Rabasović, M. D., Pantelić, D., Jelenković, B., Bugarski, B.,& Krmpot, A.. (2017). Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy. in Journal of Biomedical Optics
Spie-Soc Photo-Optical Instrumentation Engineers, Bellingham., 22(2).
https://doi.org/10.1117/1.JBO.22.2.026003

Bukara K, Jovanić S, Drvenica I, Stančić A, Ilić VL, Rabasović MD, Pantelić D, Jelenković B, Bugarski B, Krmpot A. Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy. in Journal of Biomedical Optics. 2017;22(2).
doi:10.1117/1.JBO.22.2.026003 .

Bukara, Katarina, Jovanić, Svetlana, Drvenica, Ivana, Stančić, Ana, Ilić, Vesna Lj., Rabasović, Mihailo D., Pantelić, Dejan, Jelenković, Branislav, Bugarski, Branko, Krmpot, Aleksandar, "Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy" in Journal of Biomedical Optics, 22, no. 2 (2017),
https://doi.org/10.1117/1.JBO.22.2.026003 . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Bukara, Katarina
AU  - Ilić, Vesna Lj.
AU  - Mišić, Danijela
AU  - Vasić, Borislav
AU  - Gajić, Radoš B.
AU  - Đorđević, Verica
AU  - Veljović, Đorđe
AU  - Belić, Aleksandar
AU  - Bugarski, Branko
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3329
AB  - The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37 degrees C had statistically significant effect on loaded amount of DexP (P lt 0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.
PB  - Wiley, Hoboken
T2  - Biotechnology Progress
T1  - Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate
EP  - 1055
IS  - 4
SP  - 1046
VL  - 32
DO  - 10.1002/btpr.2304
ER  - 

@article{
author = "Drvenica, Ivana and Bukara, Katarina and Ilić, Vesna Lj. and Mišić, Danijela and Vasić, Borislav and Gajić, Radoš B. and Đorđević, Verica and Veljović, Đorđe and Belić, Aleksandar and Bugarski, Branko",
year = "2016",
abstract = "The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37 degrees C had statistically significant effect on loaded amount of DexP (P lt 0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology Progress",
title = "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate",
pages = "1055-1046",
number = "4",
volume = "32",
doi = "10.1002/btpr.2304"
}

Drvenica, I., Bukara, K., Ilić, V. Lj., Mišić, D., Vasić, B., Gajić, R. B., Đorđević, V., Veljović, Đ., Belić, A.,& Bugarski, B.. (2016). Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress
Wiley, Hoboken., 32(4), 1046-1055.
https://doi.org/10.1002/btpr.2304

Drvenica I, Bukara K, Ilić VL, Mišić D, Vasić B, Gajić RB, Đorđević V, Veljović Đ, Belić A, Bugarski B. Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress. 2016;32(4):1046-1055.
doi:10.1002/btpr.2304 .

Drvenica, Ivana, Bukara, Katarina, Ilić, Vesna Lj., Mišić, Danijela, Vasić, Borislav, Gajić, Radoš B., Đorđević, Verica, Veljović, Đorđe, Belić, Aleksandar, Bugarski, Branko, "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate" in Biotechnology Progress, 32, no. 4 (2016):1046-1055,
https://doi.org/10.1002/btpr.2304 . .

TY  - JOUR
AU  - Bukara, Katarina
AU  - Drvenica, Ivana
AU  - Ilić, Vesna Lj.
AU  - Stančić, Ana
AU  - Mišić, Danijela
AU  - Vasić, Borislav
AU  - Gajić, Radoš
AU  - Vučetić, Dušan
AU  - Kiekens, Filip
AU  - Bugarski, Branko
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3318
AB  - The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials - porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process - gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Biotechnology
T1  - Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts
EP  - 22
SP  - 14
VL  - 240
DO  - 10.1016/j.jbiotec.2016.10.017
ER  - 

@article{
author = "Bukara, Katarina and Drvenica, Ivana and Ilić, Vesna Lj. and Stančić, Ana and Mišić, Danijela and Vasić, Borislav and Gajić, Radoš and Vučetić, Dušan and Kiekens, Filip and Bugarski, Branko",
year = "2016",
abstract = "The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials - porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process - gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Biotechnology",
title = "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts",
pages = "22-14",
volume = "240",
doi = "10.1016/j.jbiotec.2016.10.017"
}

Bukara, K., Drvenica, I., Ilić, V. Lj., Stančić, A., Mišić, D., Vasić, B., Gajić, R., Vučetić, D., Kiekens, F.,& Bugarski, B.. (2016). Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology
Elsevier Science Bv, Amsterdam., 240, 14-22.
https://doi.org/10.1016/j.jbiotec.2016.10.017

Bukara K, Drvenica I, Ilić VL, Stančić A, Mišić D, Vasić B, Gajić R, Vučetić D, Kiekens F, Bugarski B. Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology. 2016;240:14-22.
doi:10.1016/j.jbiotec.2016.10.017 .

Bukara, Katarina, Drvenica, Ivana, Ilić, Vesna Lj., Stančić, Ana, Mišić, Danijela, Vasić, Borislav, Gajić, Radoš, Vučetić, Dušan, Kiekens, Filip, Bugarski, Branko, "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts" in Journal of Biotechnology, 240 (2016):14-22,
https://doi.org/10.1016/j.jbiotec.2016.10.017 . .

TY  - JOUR
AU  - Kostić, Ivana T.
AU  - Ilić, Vesna Lj.
AU  - Bukara, Katarina
AU  - Mojsilović, Slavko B.
AU  - Đurić, Zorka Ž.
AU  - Draškovič, Petra
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2900
AB  - Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected.
AB  - Uprkos činjenici da su metode koje se baziraju na osmotskim osobinama ćelija najčešće korišćene metode za inkapsulaciju lekova u humane i životinjske eritrocite, podaci o osmotskim osobinama eritrocita životinjskog porekla su vrlo oskudni. Cilj ovog rada bio je ispitivanje mogućnosti korišćenja metode protočne citometrije za određivanje osmotskih osobina svinjskih i goveđih eritrocita, čime bi se olakšao inženjering pomenutih životinjskih eritrocita za otpuštanje lekova. Metodom protočne citometrije uspešno su dobijene informacije o osmotskoj fragilnosti svinjskih i goveđih eritrocita i načinjeni su početni koraci u proceni oblika velikog broja eritrocita. Iako ova metoda nije uspela da potvrdi bubrenje svinjskih eritrocita, ukazala je na razliku u uzorcima svinjskih eritrocita koji su imali osnovne hematološke parametre izvan i unutar referentnih vrednosti. U cilju primene svinjskih i goveđih eritrocita kao nosača lekova, metoda protočne citometrije je, potvrdivši prisustvo osmotski različitih frakcija eritrocita, ukazala na to da se različite količine inkapsuliranog leka u pojedinačnim, kako svinjskim, tako i goveđim eritrocitima mogu očekivati.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery
T1  - Određivanje osmotskih osobina životinjskih eritrocita protočnom citometrijom u cilju njihovog inženjeringa kao nosača lekova
EP  - 76
IS  - 1
SP  - 67
VL  - 69
DO  - 10.2298/HEMIND140124021K
ER  - 

@article{
author = "Kostić, Ivana T. and Ilić, Vesna Lj. and Bukara, Katarina and Mojsilović, Slavko B. and Đurić, Zorka Ž. and Draškovič, Petra and Bugarski, Branko",
year = "2015",
abstract = "Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected., Uprkos činjenici da su metode koje se baziraju na osmotskim osobinama ćelija najčešće korišćene metode za inkapsulaciju lekova u humane i životinjske eritrocite, podaci o osmotskim osobinama eritrocita životinjskog porekla su vrlo oskudni. Cilj ovog rada bio je ispitivanje mogućnosti korišćenja metode protočne citometrije za određivanje osmotskih osobina svinjskih i goveđih eritrocita, čime bi se olakšao inženjering pomenutih životinjskih eritrocita za otpuštanje lekova. Metodom protočne citometrije uspešno su dobijene informacije o osmotskoj fragilnosti svinjskih i goveđih eritrocita i načinjeni su početni koraci u proceni oblika velikog broja eritrocita. Iako ova metoda nije uspela da potvrdi bubrenje svinjskih eritrocita, ukazala je na razliku u uzorcima svinjskih eritrocita koji su imali osnovne hematološke parametre izvan i unutar referentnih vrednosti. U cilju primene svinjskih i goveđih eritrocita kao nosača lekova, metoda protočne citometrije je, potvrdivši prisustvo osmotski različitih frakcija eritrocita, ukazala na to da se različite količine inkapsuliranog leka u pojedinačnim, kako svinjskim, tako i goveđim eritrocitima mogu očekivati.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery, Određivanje osmotskih osobina životinjskih eritrocita protočnom citometrijom u cilju njihovog inženjeringa kao nosača lekova",
pages = "76-67",
number = "1",
volume = "69",
doi = "10.2298/HEMIND140124021K"
}

Kostić, I. T., Ilić, V. Lj., Bukara, K., Mojsilović, S. B., Đurić, Z. Ž., Draškovič, P.,& Bugarski, B.. (2015). Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. in Hemijska industrija
Association of Chemical Engineers of Serbia., 69(1), 67-76.
https://doi.org/10.2298/HEMIND140124021K

Kostić IT, Ilić VL, Bukara K, Mojsilović SB, Đurić ZŽ, Draškovič P, Bugarski B. Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. in Hemijska industrija. 2015;69(1):67-76.
doi:10.2298/HEMIND140124021K .

Kostić, Ivana T., Ilić, Vesna Lj., Bukara, Katarina, Mojsilović, Slavko B., Đurić, Zorka Ž., Draškovič, Petra, Bugarski, Branko, "Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery" in Hemijska industrija, 69, no. 1 (2015):67-76,
https://doi.org/10.2298/HEMIND140124021K . .

TY  - JOUR
AU  - Kostić, Ivana T.
AU  - Ilić, Vesna Lj.
AU  - Đorđević, Verica
AU  - Bukara, Katarina
AU  - Mojsilović, Slavko B.
AU  - Nedović, Viktor
AU  - Bugarski, Diana
AU  - Veljović, Đorđe
AU  - Misić, Danijela M.
AU  - Bugarski, Branko
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2738
AB  - The present study was aimed at investigating the effect of isolation process-gradual hypotonic hemolysis on chosen parameters of the erythrocyte membranes (ghosts) originating from bovine and porcine slaughterhouse blood. The estimation of the gradual hypotonic hemolysis as a drug loading procedure for the erythrocyte ghosts was performed as well. Based on the results derived from analysis of the osmotic properties of the erythrocytes, the gradual hemolysis was performed with high volume of erythrocytes and 35 mM hypotonic sodium-phosphate/NaCl, enabling  gt 90% of hemolysis for both types of erythrocytes. Detailed insight into ghosts' morphology by field emission-scanning electron microscopy revealed a distortion from erythrocyte shape and an altered surface texture with increased bilayer curvature for both samples. Compared to erythrocytes, an average diameter of ghosts from both type of erythrocytes decreased for only about 10%. The reported unidispersity of the isolated ghosts is of great importance for their potential application as vehicles of active compounds. Gradual hemolysis did not lead to substantial loss of cholesterol and membrane/cytoskeleton proteins. This result indicated the ghosts' possibility to mimic the chemical and structural anisotropic environment of in vivo cell membranes, which is of significance for drug diffusion and partition coefficients. Induced shift of phosphatidylserine to external surface of the ghosts demonstrated their potential application as vehicles for targeted drug delivery to cells of reticuloendothelial system. Ultra high-performance liquid chromatography and Fourier transform infrared spectroscopy revealed the presence of a drug model - dexamethasone-sodium phosphate, and its interaction with structural components in both types of erythrocyte ghosts.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization
EP  - 259
SP  - 250
VL  - 122
DO  - 10.1016/j.colsurfb.2014.06.043
ER  - 

@article{
author = "Kostić, Ivana T. and Ilić, Vesna Lj. and Đorđević, Verica and Bukara, Katarina and Mojsilović, Slavko B. and Nedović, Viktor and Bugarski, Diana and Veljović, Đorđe and Misić, Danijela M. and Bugarski, Branko",
year = "2014",
abstract = "The present study was aimed at investigating the effect of isolation process-gradual hypotonic hemolysis on chosen parameters of the erythrocyte membranes (ghosts) originating from bovine and porcine slaughterhouse blood. The estimation of the gradual hypotonic hemolysis as a drug loading procedure for the erythrocyte ghosts was performed as well. Based on the results derived from analysis of the osmotic properties of the erythrocytes, the gradual hemolysis was performed with high volume of erythrocytes and 35 mM hypotonic sodium-phosphate/NaCl, enabling  gt 90% of hemolysis for both types of erythrocytes. Detailed insight into ghosts' morphology by field emission-scanning electron microscopy revealed a distortion from erythrocyte shape and an altered surface texture with increased bilayer curvature for both samples. Compared to erythrocytes, an average diameter of ghosts from both type of erythrocytes decreased for only about 10%. The reported unidispersity of the isolated ghosts is of great importance for their potential application as vehicles of active compounds. Gradual hemolysis did not lead to substantial loss of cholesterol and membrane/cytoskeleton proteins. This result indicated the ghosts' possibility to mimic the chemical and structural anisotropic environment of in vivo cell membranes, which is of significance for drug diffusion and partition coefficients. Induced shift of phosphatidylserine to external surface of the ghosts demonstrated their potential application as vehicles for targeted drug delivery to cells of reticuloendothelial system. Ultra high-performance liquid chromatography and Fourier transform infrared spectroscopy revealed the presence of a drug model - dexamethasone-sodium phosphate, and its interaction with structural components in both types of erythrocyte ghosts.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization",
pages = "259-250",
volume = "122",
doi = "10.1016/j.colsurfb.2014.06.043"
}

Kostić, I. T., Ilić, V. Lj., Đorđević, V., Bukara, K., Mojsilović, S. B., Nedović, V., Bugarski, D., Veljović, Đ., Misić, D. M.,& Bugarski, B.. (2014). Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 122, 250-259.
https://doi.org/10.1016/j.colsurfb.2014.06.043

Kostić IT, Ilić VL, Đorđević V, Bukara K, Mojsilović SB, Nedović V, Bugarski D, Veljović Đ, Misić DM, Bugarski B. Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization. in Colloids and Surfaces B-Biointerfaces. 2014;122:250-259.
doi:10.1016/j.colsurfb.2014.06.043 .

Kostić, Ivana T., Ilić, Vesna Lj., Đorđević, Verica, Bukara, Katarina, Mojsilović, Slavko B., Nedović, Viktor, Bugarski, Diana, Veljović, Đorđe, Misić, Danijela M., Bugarski, Branko, "Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization" in Colloids and Surfaces B-Biointerfaces, 122 (2014):250-259,
https://doi.org/10.1016/j.colsurfb.2014.06.043 . .

TY  - JOUR
AU  - Stojanović, Radoslava
AU  - Ilić, Vesna Lj.
AU  - Manojlović, Verica
AU  - Bugarski, Diana
AU  - Dević, Marija
AU  - Bugarski, Branko
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2252
AB  - In this work, we describe an optimized procedure based on gradual hemolysis for the isolation of hemoglobin derived from bovine slaughterhouse erythrocytes in a membrane bioreactor. The membrane bioreactor system that provided high yields of hemoglobin (mainly oxyhemoglobin derivate) and its separation from the empty erythrocyte membranes (ghosts) was designed at a pilot scale. Ten different concentrations of hypotonic media were assessed from the aspect of the extent of hemolysis, hematocrit values of the erythrocyte suspensions, cell swelling, and membrane deformations induced by decreased salt concentration. Effective gradual osmotic hemolysis with an extent of hemolysis of 88% was performed using 35 mM Na-phosphate/NaCl buffer of pH 7.2-7.4. Under these conditions most of the cell membranes presented the appearance of the normal ghosts under phase contrast microscope. The hemoglobin purity of  gt 80% was confirmed by SDS-PAGE. Kinetic studies showed that maximal concentration of hemoglobin was reached after 40 min, but the process cycle at which recovery of 83% was achieved lasted for 90 min. The dynamics of both steps, (1) transport through the membrane of erythrocytes during process of hemolysis and (2) transport through the reactor filters, were evaluated.
PB  - Springer, New York
T2  - Applied Biochemistry and Biotechnology
T1  - Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor
EP  - 1506
IS  - 6
SP  - 1491
VL  - 166
DO  - 10.1007/s12010-012-9543-9
ER  - 

@article{
author = "Stojanović, Radoslava and Ilić, Vesna Lj. and Manojlović, Verica and Bugarski, Diana and Dević, Marija and Bugarski, Branko",
year = "2012",
abstract = "In this work, we describe an optimized procedure based on gradual hemolysis for the isolation of hemoglobin derived from bovine slaughterhouse erythrocytes in a membrane bioreactor. The membrane bioreactor system that provided high yields of hemoglobin (mainly oxyhemoglobin derivate) and its separation from the empty erythrocyte membranes (ghosts) was designed at a pilot scale. Ten different concentrations of hypotonic media were assessed from the aspect of the extent of hemolysis, hematocrit values of the erythrocyte suspensions, cell swelling, and membrane deformations induced by decreased salt concentration. Effective gradual osmotic hemolysis with an extent of hemolysis of 88% was performed using 35 mM Na-phosphate/NaCl buffer of pH 7.2-7.4. Under these conditions most of the cell membranes presented the appearance of the normal ghosts under phase contrast microscope. The hemoglobin purity of  gt 80% was confirmed by SDS-PAGE. Kinetic studies showed that maximal concentration of hemoglobin was reached after 40 min, but the process cycle at which recovery of 83% was achieved lasted for 90 min. The dynamics of both steps, (1) transport through the membrane of erythrocytes during process of hemolysis and (2) transport through the reactor filters, were evaluated.",
publisher = "Springer, New York",
journal = "Applied Biochemistry and Biotechnology",
title = "Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor",
pages = "1506-1491",
number = "6",
volume = "166",
doi = "10.1007/s12010-012-9543-9"
}

Stojanović, R., Ilić, V. Lj., Manojlović, V., Bugarski, D., Dević, M.,& Bugarski, B.. (2012). Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor. in Applied Biochemistry and Biotechnology
Springer, New York., 166(6), 1491-1506.
https://doi.org/10.1007/s12010-012-9543-9

Stojanović R, Ilić VL, Manojlović V, Bugarski D, Dević M, Bugarski B. Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor. in Applied Biochemistry and Biotechnology. 2012;166(6):1491-1506.
doi:10.1007/s12010-012-9543-9 .

Stojanović, Radoslava, Ilić, Vesna Lj., Manojlović, Verica, Bugarski, Diana, Dević, Marija, Bugarski, Branko, "Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor" in Applied Biochemistry and Biotechnology, 166, no. 6 (2012):1491-1506,
https://doi.org/10.1007/s12010-012-9543-9 . .

TY  - JOUR
AU  - Pravilović, Radoslava
AU  - Mojsilović, Slavko B.
AU  - Kostić, Ivana T.
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Diana
AU  - Đorđević, Verica
AU  - Bugarski, Branko
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2103
AB  - In this work, we describe an optimized procedure based on gradual hemolysis for the isolation of hemoglobin derived from bovine slaughterhouse erythrocytes in a membrane bioreactor. The membrane bioreactor system provided high yields of hemoglobin (mainly oxyhemoglobin derivate) and its separation from the empty erythrocyte membranes (ghosts). Ten different concentrations of hypotonic media were assessed from the aspect of the extent of hemolysis, hematocrit values of the erythrocyte suspensions, cell swelling and membrane deformations induced by decreased salt concentration. Effective gradual osmotic hemolysis with an extent of hemolysis of 83% was performed using 35 mM Na-phosphate/NaCl buffer of pH 7.2-7.4. Under these conditions most of the cell membranes presented the appearance of the normal ghosts under phase contrast microscope. The results show that isolation process yielded predominantly to oxyhemoglobin. Kinetic studies showed that maximal concentration of hemoglobin was reached after 40 min, but the process cycle at which recovery of 83% was achieved lasted for 90 min.
AB  - Dugi niz godina, i kod nas i u svetu, razvijaju se tehnološki postupci za izolovanje strukturno očuvanog i biološki aktivnog hemoglobina koji bi mogao da se koristi kao zamenik za krv, kao izvor biološki visokoaktivnog hemskog gvožđa u prevenciji anemije kod ljudi i životinja, ili kao reagens u dijagnostici. U ovom radu optimizovan je postupak za izolovanje hemoglobina iz eritrocita poreklom iz otpadne klanične goveđe krvi sa ciljem dobijanja preparata hemskog gvožđa za prevenciju anemije kod životinja. Testirana je osetljivost goveđih eritrocita na osmotsku lizu sa ciljem definisanja optimalnog puferskog sistema za efikasno izvođenje kontrolisane hemolize. Dobijeni rezultati su pokazali da goveđi eritrociti imaju povećanu osmotsku osetljivost u odnosu na humane eritrocite, a da je optimalan puferski sistem za izvođenje njihove kontrolisane hemolize 35 mM natrijum-fosfatni/NaCl pufer pH 7,2-7,4. Kontrolisana hemoliza sa optimizovanim puferskim sistemom je izvedena u membranskom reaktorskom sistemu i ostvaren je prinos hemoglobina od 83±12%. Tokom ovog procesa nije došlo do oštećenja membrane eritrocita, a intaktne membrane praznih eritrocita bi se mogle koristiti za inkapsulaciju biološki aktivnih supstanci.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes
T1  - Optimizacija procesa izolovanja hemoglobina iz goveđih eritrocita kontrolisanom hemolizom
EP  - 529
IS  - 4
SP  - 519
VL  - 66
DO  - 10.2298/HEMIND111122008S
ER  - 

@article{
author = "Pravilović, Radoslava and Mojsilović, Slavko B. and Kostić, Ivana T. and Ilić, Vesna Lj. and Bugarski, Diana and Đorđević, Verica and Bugarski, Branko",
year = "2012",
abstract = "In this work, we describe an optimized procedure based on gradual hemolysis for the isolation of hemoglobin derived from bovine slaughterhouse erythrocytes in a membrane bioreactor. The membrane bioreactor system provided high yields of hemoglobin (mainly oxyhemoglobin derivate) and its separation from the empty erythrocyte membranes (ghosts). Ten different concentrations of hypotonic media were assessed from the aspect of the extent of hemolysis, hematocrit values of the erythrocyte suspensions, cell swelling and membrane deformations induced by decreased salt concentration. Effective gradual osmotic hemolysis with an extent of hemolysis of 83% was performed using 35 mM Na-phosphate/NaCl buffer of pH 7.2-7.4. Under these conditions most of the cell membranes presented the appearance of the normal ghosts under phase contrast microscope. The results show that isolation process yielded predominantly to oxyhemoglobin. Kinetic studies showed that maximal concentration of hemoglobin was reached after 40 min, but the process cycle at which recovery of 83% was achieved lasted for 90 min., Dugi niz godina, i kod nas i u svetu, razvijaju se tehnološki postupci za izolovanje strukturno očuvanog i biološki aktivnog hemoglobina koji bi mogao da se koristi kao zamenik za krv, kao izvor biološki visokoaktivnog hemskog gvožđa u prevenciji anemije kod ljudi i životinja, ili kao reagens u dijagnostici. U ovom radu optimizovan je postupak za izolovanje hemoglobina iz eritrocita poreklom iz otpadne klanične goveđe krvi sa ciljem dobijanja preparata hemskog gvožđa za prevenciju anemije kod životinja. Testirana je osetljivost goveđih eritrocita na osmotsku lizu sa ciljem definisanja optimalnog puferskog sistema za efikasno izvođenje kontrolisane hemolize. Dobijeni rezultati su pokazali da goveđi eritrociti imaju povećanu osmotsku osetljivost u odnosu na humane eritrocite, a da je optimalan puferski sistem za izvođenje njihove kontrolisane hemolize 35 mM natrijum-fosfatni/NaCl pufer pH 7,2-7,4. Kontrolisana hemoliza sa optimizovanim puferskim sistemom je izvedena u membranskom reaktorskom sistemu i ostvaren je prinos hemoglobina od 83±12%. Tokom ovog procesa nije došlo do oštećenja membrane eritrocita, a intaktne membrane praznih eritrocita bi se mogle koristiti za inkapsulaciju biološki aktivnih supstanci.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes, Optimizacija procesa izolovanja hemoglobina iz goveđih eritrocita kontrolisanom hemolizom",
pages = "529-519",
number = "4",
volume = "66",
doi = "10.2298/HEMIND111122008S"
}

Pravilović, R., Mojsilović, S. B., Kostić, I. T., Ilić, V. Lj., Bugarski, D., Đorđević, V.,& Bugarski, B.. (2012). Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes. in Hemijska industrija
Association of Chemical Engineers of Serbia., 66(4), 519-529.
https://doi.org/10.2298/HEMIND111122008S

Pravilović R, Mojsilović SB, Kostić IT, Ilić VL, Bugarski D, Đorđević V, Bugarski B. Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes. in Hemijska industrija. 2012;66(4):519-529.
doi:10.2298/HEMIND111122008S .

Pravilović, Radoslava, Mojsilović, Slavko B., Kostić, Ivana T., Ilić, Vesna Lj., Bugarski, Diana, Đorđević, Verica, Bugarski, Branko, "Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes" in Hemijska industrija, 66, no. 4 (2012):519-529,
https://doi.org/10.2298/HEMIND111122008S . .

TY  - JOUR
AU  - Kostić, Ivana T.
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Branko
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2063
AB  - The erythrocytes are the most abundant cellular components ( gt 99%) of blood in humans. Besides their well known physiological functions, they serve as a natural blood compartment participating in biodistribution, metabolism and action of certain drugs. On the other hand, the erythrocytes possess potential carrier capabilities and can be used for the controlled and targeted delivery of various bioactive compounds, including peptides and genetic materials. The erythrocytes feature some unique advantages compared to other delivery systems, such as high biocompatibility, biodegradability, long life­span, remarkable decrease in concentration fluctuations in steady state in comparison to the conventional methods of drug administration etc. This short review accentuate the most significant experimental results and perspectives of medical applications of novel drug delivery systems based on erythrocytes. .
AB  - Eritrociti predstavljaju najzastupljenije ćelijske komponente krvi ( gt 99%) kod ljudi. Pored dobro poznatih fizioloških funkcija koje vrše, oni predstavljaju i prirodne kompartmane krvi koje učestvuju u biodistribuciji, metabolizmu i delovanju određenih lekovitih supstanci. S druge strane, eritrociti poseduju ogroman potencijal kao nosači za kontrolisanu i ciljanu isporuku različitih bioaktivnih jedinjenja, uključujući i peptide i genetički materijal. Produženi životni vek u cirkulaciji, dostupnost, visoka biokompatibilnost i prirodni mehanizmi za bezbednu eliminaciju, kao i značajno smanjenje fluktuacije koncentracije lekovite supstance u plazmi, čine jedinstvene prednosti eritrocita kao nosača lekovitih supstanci u poređenju sa konvencionalnim načinima administracije lekova. Ovaj kratki pregled ističe najznačajnije eksperimentalne rezultate i izglede za primenu eritrocita kao savremenih nosača lekovitih supstanci u medicini. .
PB  - MOST ART d.o.o., Beograd
T2  - MD - Medical data
T1  - Erythrocytes as a novel drug delivery systems: Current status, challenges and perspectives of medical applications
T1  - Eritrociti kao savremeni nosači lekovitih supstanci - trenutni status, izazovi i izgledi za primenu u medicini
EP  - 292
IS  - 3
SP  - 287
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2063
ER  - 

@article{
author = "Kostić, Ivana T. and Ilić, Vesna Lj. and Bugarski, Branko",
year = "2012",
abstract = "The erythrocytes are the most abundant cellular components ( gt 99%) of blood in humans. Besides their well known physiological functions, they serve as a natural blood compartment participating in biodistribution, metabolism and action of certain drugs. On the other hand, the erythrocytes possess potential carrier capabilities and can be used for the controlled and targeted delivery of various bioactive compounds, including peptides and genetic materials. The erythrocytes feature some unique advantages compared to other delivery systems, such as high biocompatibility, biodegradability, long life­span, remarkable decrease in concentration fluctuations in steady state in comparison to the conventional methods of drug administration etc. This short review accentuate the most significant experimental results and perspectives of medical applications of novel drug delivery systems based on erythrocytes. ., Eritrociti predstavljaju najzastupljenije ćelijske komponente krvi ( gt 99%) kod ljudi. Pored dobro poznatih fizioloških funkcija koje vrše, oni predstavljaju i prirodne kompartmane krvi koje učestvuju u biodistribuciji, metabolizmu i delovanju određenih lekovitih supstanci. S druge strane, eritrociti poseduju ogroman potencijal kao nosači za kontrolisanu i ciljanu isporuku različitih bioaktivnih jedinjenja, uključujući i peptide i genetički materijal. Produženi životni vek u cirkulaciji, dostupnost, visoka biokompatibilnost i prirodni mehanizmi za bezbednu eliminaciju, kao i značajno smanjenje fluktuacije koncentracije lekovite supstance u plazmi, čine jedinstvene prednosti eritrocita kao nosača lekovitih supstanci u poređenju sa konvencionalnim načinima administracije lekova. Ovaj kratki pregled ističe najznačajnije eksperimentalne rezultate i izglede za primenu eritrocita kao savremenih nosača lekovitih supstanci u medicini. .",
publisher = "MOST ART d.o.o., Beograd",
journal = "MD - Medical data",
title = "Erythrocytes as a novel drug delivery systems: Current status, challenges and perspectives of medical applications, Eritrociti kao savremeni nosači lekovitih supstanci - trenutni status, izazovi i izgledi za primenu u medicini",
pages = "292-287",
number = "3",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2063"
}

Kostić, I. T., Ilić, V. Lj.,& Bugarski, B.. (2012). Erythrocytes as a novel drug delivery systems: Current status, challenges and perspectives of medical applications. in MD - Medical data
MOST ART d.o.o., Beograd., 4(3), 287-292.
https://hdl.handle.net/21.15107/rcub_technorep_2063

Kostić IT, Ilić VL, Bugarski B. Erythrocytes as a novel drug delivery systems: Current status, challenges and perspectives of medical applications. in MD - Medical data. 2012;4(3):287-292.
https://hdl.handle.net/21.15107/rcub_technorep_2063 .

Kostić, Ivana T., Ilić, Vesna Lj., Bugarski, Branko, "Erythrocytes as a novel drug delivery systems: Current status, challenges and perspectives of medical applications" in MD - Medical data, 4, no. 3 (2012):287-292,
https://hdl.handle.net/21.15107/rcub_technorep_2063 .

TY  - CONF
AU  - Mihailović, Darka
AU  - Šaponjic, Zoran
AU  - Vodnik, Vesna
AU  - Ilić, Vesna
AU  - Potkonjak, Branislav
AU  - Jovančić, Petar
AU  - Nedeljković, Jovan
AU  - Radetić, Maja
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6494
AB  - This study discusses the possibility of engineering the multifunctional textile nanocomposite material based on the polyester (PES) fabric modified with colloidal Ag and TiO2 nanoparticles (NPs). Namely, encouraging results on the effects of TiO2 and Ag NPs separately applied to textile materials in our previous studies were the basis for an assumption that combined treatment with Ag and TiO2 NPs may bring multifunctional textile nanocomposite materials with desirable UV protective and antimicrobial effects. The antimicrobial activity of differently modified PES fabrics was tested against Gram-negative bacterium E. coli, Gram-positive bacterium S. aureus and fungus C. albicans. The efficiency of UV blocking was evaluated by determining the UV protection factor (UPF) of PES fabrics. The effect of Ag NPs on the photoactivity of TiO2 NPs deposited onto the PES fabrics was tested by degradation of methylene blue in aqueous solution under UV illumination. The total content of Ag and Ti in the PES fabrics was determined by atomic absorption spectroscopy. The morphology of PES fibers was followed by SEM.

The order of Ag and TiO2 NPs loading affects the antimicrobial efficiency of PES fabrics. The fabrics provide maximum UV protection independently of the order of Ag and TiO2 NPs loading. Ag NPs positively influenced the photodegradation activity of TiO2 NPs and total photodegradation of methylene blue under UV illumination was achieved after 24 h.
PB  - Dokuz Eylül University, Faculty of Engineering, Department of Textile Engineering, Istanbul
C3  - Proceedings 4th International Technical Textiles Congress, 16-18 May, 2010, Istanbul, Turkey
T1  - Functionalization of Polyester Fabrics with Ag and TiO2 Nanoparticles
EP  - 7
SP  - 1
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6494
ER  - 

@conference{
author = "Mihailović, Darka and Šaponjic, Zoran and Vodnik, Vesna and Ilić, Vesna and Potkonjak, Branislav and Jovančić, Petar and Nedeljković, Jovan and Radetić, Maja",
year = "2010",
abstract = "This study discusses the possibility of engineering the multifunctional textile nanocomposite material based on the polyester (PES) fabric modified with colloidal Ag and TiO2 nanoparticles (NPs). Namely, encouraging results on the effects of TiO2 and Ag NPs separately applied to textile materials in our previous studies were the basis for an assumption that combined treatment with Ag and TiO2 NPs may bring multifunctional textile nanocomposite materials with desirable UV protective and antimicrobial effects. The antimicrobial activity of differently modified PES fabrics was tested against Gram-negative bacterium E. coli, Gram-positive bacterium S. aureus and fungus C. albicans. The efficiency of UV blocking was evaluated by determining the UV protection factor (UPF) of PES fabrics. The effect of Ag NPs on the photoactivity of TiO2 NPs deposited onto the PES fabrics was tested by degradation of methylene blue in aqueous solution under UV illumination. The total content of Ag and Ti in the PES fabrics was determined by atomic absorption spectroscopy. The morphology of PES fibers was followed by SEM.

The order of Ag and TiO2 NPs loading affects the antimicrobial efficiency of PES fabrics. The fabrics provide maximum UV protection independently of the order of Ag and TiO2 NPs loading. Ag NPs positively influenced the photodegradation activity of TiO2 NPs and total photodegradation of methylene blue under UV illumination was achieved after 24 h.",
publisher = "Dokuz Eylül University, Faculty of Engineering, Department of Textile Engineering, Istanbul",
journal = "Proceedings 4th International Technical Textiles Congress, 16-18 May, 2010, Istanbul, Turkey",
title = "Functionalization of Polyester Fabrics with Ag and TiO2 Nanoparticles",
pages = "7-1",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6494"
}

Mihailović, D., Šaponjic, Z., Vodnik, V., Ilić, V., Potkonjak, B., Jovančić, P., Nedeljković, J.,& Radetić, M.. (2010). Functionalization of Polyester Fabrics with Ag and TiO2 Nanoparticles. in Proceedings 4th International Technical Textiles Congress, 16-18 May, 2010, Istanbul, Turkey
Dokuz Eylül University, Faculty of Engineering, Department of Textile Engineering, Istanbul., 1-7.
https://hdl.handle.net/21.15107/rcub_technorep_6494

Mihailović D, Šaponjic Z, Vodnik V, Ilić V, Potkonjak B, Jovančić P, Nedeljković J, Radetić M. Functionalization of Polyester Fabrics with Ag and TiO2 Nanoparticles. in Proceedings 4th International Technical Textiles Congress, 16-18 May, 2010, Istanbul, Turkey. 2010;:1-7.
https://hdl.handle.net/21.15107/rcub_technorep_6494 .

Mihailović, Darka, Šaponjic, Zoran, Vodnik, Vesna, Ilić, Vesna, Potkonjak, Branislav, Jovančić, Petar, Nedeljković, Jovan, Radetić, Maja, "Functionalization of Polyester Fabrics with Ag and TiO2 Nanoparticles" in Proceedings 4th International Technical Textiles Congress, 16-18 May, 2010, Istanbul, Turkey (2010):1-7,
https://hdl.handle.net/21.15107/rcub_technorep_6494 .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Branko
AU  - Plavšić, Milenko B.
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1706
AB  - The complex phenomenon of rearrangement of band 3 molecules after erythrocyte swelling under hypotonic condition is considered. The rearrangement includes the increase of the mobile fraction and clustering of band 3. The self-associative tendency and the action of the elastic field generated within the lipid membrane after erythrocyte swelling result in equilibration of the number of molecules per cluster and the number of clusters. The local perturbation of the elastic field induces excitation of the cluster in the nearest neighbor and changes its packing state generating changes in the free volume within the cluster. The local perturbation could result in the reversible formation of osmotic hole. We formulated a model to predict changes of the cluster packing states generated by rearrangement of band 3 molecules on two time-scales. The phenomenon is examined on the basis of two experimental sets, i.e. low (5.2 mM Na3PO4 solution) and high (46.0 mM Na3PO4 solution) hypotonicities at 21A degrees C, from Golan and Veatch (Proc Natl Acad Sci 77(5):2537-2541, 1980). Modeling considerations suggested that lower hypotonic conditions resulted in higher values of: the driving force of agglomeration of band 3 as a measure of self-associative tendency, the specific rate of cluster breaking, the specific rate of increase of the mobile fraction of band 3, and the dispersion of cluster sizes. Lower hypotonic conditions ensure the generation of a higher average value of the free energy within the membrane after erythrocyte swelling, which enables more intensive rearrangement of band 3 molecules.
PB  - Springer, New York
T2  - European Biophysics Journal with Biophysics Letters
T1  - Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions
EP  - 800
IS  - 5
SP  - 789
VL  - 39
DO  - 10.1007/s00249-009-0554-6
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Ilić, Vesna Lj. and Bugarski, Branko and Plavšić, Milenko B.",
year = "2010",
abstract = "The complex phenomenon of rearrangement of band 3 molecules after erythrocyte swelling under hypotonic condition is considered. The rearrangement includes the increase of the mobile fraction and clustering of band 3. The self-associative tendency and the action of the elastic field generated within the lipid membrane after erythrocyte swelling result in equilibration of the number of molecules per cluster and the number of clusters. The local perturbation of the elastic field induces excitation of the cluster in the nearest neighbor and changes its packing state generating changes in the free volume within the cluster. The local perturbation could result in the reversible formation of osmotic hole. We formulated a model to predict changes of the cluster packing states generated by rearrangement of band 3 molecules on two time-scales. The phenomenon is examined on the basis of two experimental sets, i.e. low (5.2 mM Na3PO4 solution) and high (46.0 mM Na3PO4 solution) hypotonicities at 21A degrees C, from Golan and Veatch (Proc Natl Acad Sci 77(5):2537-2541, 1980). Modeling considerations suggested that lower hypotonic conditions resulted in higher values of: the driving force of agglomeration of band 3 as a measure of self-associative tendency, the specific rate of cluster breaking, the specific rate of increase of the mobile fraction of band 3, and the dispersion of cluster sizes. Lower hypotonic conditions ensure the generation of a higher average value of the free energy within the membrane after erythrocyte swelling, which enables more intensive rearrangement of band 3 molecules.",
publisher = "Springer, New York",
journal = "European Biophysics Journal with Biophysics Letters",
title = "Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions",
pages = "800-789",
number = "5",
volume = "39",
doi = "10.1007/s00249-009-0554-6"
}

Pajić-Lijaković, I., Ilić, V. Lj., Bugarski, B.,& Plavšić, M. B.. (2010). Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions. in European Biophysics Journal with Biophysics Letters
Springer, New York., 39(5), 789-800.
https://doi.org/10.1007/s00249-009-0554-6

Pajić-Lijaković I, Ilić VL, Bugarski B, Plavšić MB. Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions. in European Biophysics Journal with Biophysics Letters. 2010;39(5):789-800.
doi:10.1007/s00249-009-0554-6 .

Pajić-Lijaković, Ivana, Ilić, Vesna Lj., Bugarski, Branko, Plavšić, Milenko B., "Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions" in European Biophysics Journal with Biophysics Letters, 39, no. 5 (2010):789-800,
https://doi.org/10.1007/s00249-009-0554-6 . .

TY  - CONF
AU  - Ilić, Vesna
AU  - Šaponjic, Zoran
AU  - Vodnik, Vesna
AU  - Lazović, Saša
AU  - Potkonjak, Branislav
AU  - Jovančić, Petar
AU  - Nedeljković, Jovan
AU  - Radetić, Maja
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6500
AB  - The aim of this study was to highlight the possibility of using the air RF plasma for fiber surface activation that can facilitate the deposition of silver nanoparticles from colloid onto the polyester fabrics and thus, enhance their antibacterial properties. The deposition of silver nanoparticles caused significant morphological changes as well as the color change of polyester fabrics. Untreated polyester fabrics and polyester fabrics activated by air RF plasma, both, subsequently modified by silver nanoparticles exhibited excellent antibacterial properties against Gram-negative bacterium E. coli and Gram-positive bacterium S. aureus. However, polyester fabrics activated by plasma provided better laundering durability of achieved effects.
PB  - University of Gent : Department of Materials, Textiles and Chemical Engineering
C3  - Proceedings International Conference: Latest Advances in High Tech Textiles and Textile-based Materials, 23-25 September, 2009, Ghent, Belgium
T1  - The antibacterial efficiency of polyester fabrics modified by air RF plasma and silver nanoparticles
EP  - 31
SP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6500
ER  - 

@conference{
author = "Ilić, Vesna and Šaponjic, Zoran and Vodnik, Vesna and Lazović, Saša and Potkonjak, Branislav and Jovančić, Petar and Nedeljković, Jovan and Radetić, Maja",
year = "2009",
abstract = "The aim of this study was to highlight the possibility of using the air RF plasma for fiber surface activation that can facilitate the deposition of silver nanoparticles from colloid onto the polyester fabrics and thus, enhance their antibacterial properties. The deposition of silver nanoparticles caused significant morphological changes as well as the color change of polyester fabrics. Untreated polyester fabrics and polyester fabrics activated by air RF plasma, both, subsequently modified by silver nanoparticles exhibited excellent antibacterial properties against Gram-negative bacterium E. coli and Gram-positive bacterium S. aureus. However, polyester fabrics activated by plasma provided better laundering durability of achieved effects.",
publisher = "University of Gent : Department of Materials, Textiles and Chemical Engineering",
journal = "Proceedings International Conference: Latest Advances in High Tech Textiles and Textile-based Materials, 23-25 September, 2009, Ghent, Belgium",
title = "The antibacterial efficiency of polyester fabrics modified by air RF plasma and silver nanoparticles",
pages = "31-26",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6500"
}

Ilić, V., Šaponjic, Z., Vodnik, V., Lazović, S., Potkonjak, B., Jovančić, P., Nedeljković, J.,& Radetić, M.. (2009). The antibacterial efficiency of polyester fabrics modified by air RF plasma and silver nanoparticles. in Proceedings International Conference: Latest Advances in High Tech Textiles and Textile-based Materials, 23-25 September, 2009, Ghent, Belgium
University of Gent : Department of Materials, Textiles and Chemical Engineering., 26-31.
https://hdl.handle.net/21.15107/rcub_technorep_6500

Ilić V, Šaponjic Z, Vodnik V, Lazović S, Potkonjak B, Jovančić P, Nedeljković J, Radetić M. The antibacterial efficiency of polyester fabrics modified by air RF plasma and silver nanoparticles. in Proceedings International Conference: Latest Advances in High Tech Textiles and Textile-based Materials, 23-25 September, 2009, Ghent, Belgium. 2009;:26-31.
https://hdl.handle.net/21.15107/rcub_technorep_6500 .

Ilić, Vesna, Šaponjic, Zoran, Vodnik, Vesna, Lazović, Saša, Potkonjak, Branislav, Jovančić, Petar, Nedeljković, Jovan, Radetić, Maja, "The antibacterial efficiency of polyester fabrics modified by air RF plasma and silver nanoparticles" in Proceedings International Conference: Latest Advances in High Tech Textiles and Textile-based Materials, 23-25 September, 2009, Ghent, Belgium (2009):26-31,
https://hdl.handle.net/21.15107/rcub_technorep_6500 .

TY  - CONF
AU  - Ilić, Vesna
AU  - Šaponjic, Zoran
AU  - Mihailović, Darka
AU  - Molina, Ricardo
AU  - Jovančić, Petar
AU  - Nedeljković
AU  - Bokorov, Miloš
AU  - Radetić, Maja
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6521
PB  - Srpsko hemijsko društvo
C3  - 47. Savetovanje Srpskog Hemijskog Društva, Beograd, 21. 03. 2009.
T1  - Ispitivanje morfologije i hemijske strukture PA i PES vlakana modifikovanih korona pražnjenjem na atmosferskom pritisku
EP  - 229
SP  - 226
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6521
ER  - 

@conference{
author = "Ilić, Vesna and Šaponjic, Zoran and Mihailović, Darka and Molina, Ricardo and Jovančić, Petar and Nedeljković and Bokorov, Miloš and Radetić, Maja",
year = "2009",
publisher = "Srpsko hemijsko društvo",
journal = "47. Savetovanje Srpskog Hemijskog Društva, Beograd, 21. 03. 2009.",
title = "Ispitivanje morfologije i hemijske strukture PA i PES vlakana modifikovanih korona pražnjenjem na atmosferskom pritisku",
pages = "229-226",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6521"
}

Ilić, V., Šaponjic, Z., Mihailović, D., Molina, R., Jovančić, P., Nedeljković, Bokorov, M.,& Radetić, M.. (2009). Ispitivanje morfologije i hemijske strukture PA i PES vlakana modifikovanih korona pražnjenjem na atmosferskom pritisku. in 47. Savetovanje Srpskog Hemijskog Društva, Beograd, 21. 03. 2009.
Srpsko hemijsko društvo., 226-229.
https://hdl.handle.net/21.15107/rcub_technorep_6521

Ilić V, Šaponjic Z, Mihailović D, Molina R, Jovančić P, Nedeljković, Bokorov M, Radetić M. Ispitivanje morfologije i hemijske strukture PA i PES vlakana modifikovanih korona pražnjenjem na atmosferskom pritisku. in 47. Savetovanje Srpskog Hemijskog Društva, Beograd, 21. 03. 2009.. 2009;:226-229.
https://hdl.handle.net/21.15107/rcub_technorep_6521 .

Ilić, Vesna, Šaponjic, Zoran, Mihailović, Darka, Molina, Ricardo, Jovančić, Petar, Nedeljković, Bokorov, Miloš, Radetić, Maja, "Ispitivanje morfologije i hemijske strukture PA i PES vlakana modifikovanih korona pražnjenjem na atmosferskom pritisku" in 47. Savetovanje Srpskog Hemijskog Društva, Beograd, 21. 03. 2009. (2009):226-229,
https://hdl.handle.net/21.15107/rcub_technorep_6521 .

TY  - CONF
AU  - Mihailović, Darka
AU  - Radetić, Maja
AU  - Ilić, Vesna
AU  - Stanković, Snežana
AU  - Jovančić, Petar
AU  - Potkonjak, Branislav
AU  - Nedeljković, Jovan
AU  - Šaponjic, Zoran
PY  - 2008
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6507
AB  - This study is aimed to highlight the possibility of using a corona treatment for fiber surface activation, which can facilitate the loading of colloidal TiO2 nanoparticles onto the polyester fabrics and thus improve their antibacterial and self-cleaning properties. The results indicate that the antibacterial efficiency against Gram-negative bacterium Escherichia coli is highly affected by the nature of colloidal TiO2 nanoparticles. Illuminated polyester fabrics loaded with TiO2 nanoparticles exhibit satisfactory self-cleaning properties efficiency.
PB  - Institute of Textile Machinery and High Performance Materials Technology (ITM), Dresden
C3  - Proc. 2nd Aachen-Dresden International Textile Conference, 4-5 December, 2008, Dresden, Germany
T1  - Modification of Corona Pretreated Polyester fabrics with Colloidal TiO2 Nanoparticles for Imparting Specific Properties
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6507
ER  - 

@conference{
author = "Mihailović, Darka and Radetić, Maja and Ilić, Vesna and Stanković, Snežana and Jovančić, Petar and Potkonjak, Branislav and Nedeljković, Jovan and Šaponjic, Zoran",
year = "2008",
abstract = "This study is aimed to highlight the possibility of using a corona treatment for fiber surface activation, which can facilitate the loading of colloidal TiO2 nanoparticles onto the polyester fabrics and thus improve their antibacterial and self-cleaning properties. The results indicate that the antibacterial efficiency against Gram-negative bacterium Escherichia coli is highly affected by the nature of colloidal TiO2 nanoparticles. Illuminated polyester fabrics loaded with TiO2 nanoparticles exhibit satisfactory self-cleaning properties efficiency.",
publisher = "Institute of Textile Machinery and High Performance Materials Technology (ITM), Dresden",
journal = "Proc. 2nd Aachen-Dresden International Textile Conference, 4-5 December, 2008, Dresden, Germany",
title = "Modification of Corona Pretreated Polyester fabrics with Colloidal TiO2 Nanoparticles for Imparting Specific Properties",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6507"
}

Mihailović, D., Radetić, M., Ilić, V., Stanković, S., Jovančić, P., Potkonjak, B., Nedeljković, J.,& Šaponjic, Z.. (2008). Modification of Corona Pretreated Polyester fabrics with Colloidal TiO2 Nanoparticles for Imparting Specific Properties. in Proc. 2nd Aachen-Dresden International Textile Conference, 4-5 December, 2008, Dresden, Germany
Institute of Textile Machinery and High Performance Materials Technology (ITM), Dresden..
https://hdl.handle.net/21.15107/rcub_technorep_6507

Mihailović D, Radetić M, Ilić V, Stanković S, Jovančić P, Potkonjak B, Nedeljković J, Šaponjic Z. Modification of Corona Pretreated Polyester fabrics with Colloidal TiO2 Nanoparticles for Imparting Specific Properties. in Proc. 2nd Aachen-Dresden International Textile Conference, 4-5 December, 2008, Dresden, Germany. 2008;.
https://hdl.handle.net/21.15107/rcub_technorep_6507 .

Mihailović, Darka, Radetić, Maja, Ilić, Vesna, Stanković, Snežana, Jovančić, Petar, Potkonjak, Branislav, Nedeljković, Jovan, Šaponjic, Zoran, "Modification of Corona Pretreated Polyester fabrics with Colloidal TiO2 Nanoparticles for Imparting Specific Properties" in Proc. 2nd Aachen-Dresden International Textile Conference, 4-5 December, 2008, Dresden, Germany (2008),
https://hdl.handle.net/21.15107/rcub_technorep_6507 .

TY  - CONF
AU  - Šaponjic, Zoran
AU  - Ilić, Vesna
AU  - Vodnik, Vesna
AU  - Molina, Ricardo
AU  - Mihailović, Darka
AU  - Jovančić, Petar
AU  - Nedeljković, Jovan
AU  - Radetić, Maja
PY  - 2008
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6514
AB  - The possibility of using a corona treatment for fibre surface activation, which can facilitate the loading of colloidal Ag nanoparticles onto the polyester fabrics and thus enhance their antimicrobial activity, was studied. Antimicrobial efficiency and its laundering durability of polyester fabrics loaded with Ag nanoparticles against Gram-negative bacterium Escherichia coli, Gram-positive bacterium Staphylococcus aureus and fungus Candida albicans were examined. The deposition of colloidal Ag nanoparticles onto corona pretreated polyester fabrics provides excellent antimicrobial activity and satisfactory laundering durability of obtained effects.
C3  - Proc. 2nd Aachen-Dresden International Textile Conference, 4-5 December, 2008, Dresden, Germany
T1  - The Antimicrobial Efficiency of Corona Pretreated Polyester Fabrics Loaded with Silver Nanoparticles
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6514
ER  - 

@conference{
author = "Šaponjic, Zoran and Ilić, Vesna and Vodnik, Vesna and Molina, Ricardo and Mihailović, Darka and Jovančić, Petar and Nedeljković, Jovan and Radetić, Maja",
year = "2008",
abstract = "The possibility of using a corona treatment for fibre surface activation, which can facilitate the loading of colloidal Ag nanoparticles onto the polyester fabrics and thus enhance their antimicrobial activity, was studied. Antimicrobial efficiency and its laundering durability of polyester fabrics loaded with Ag nanoparticles against Gram-negative bacterium Escherichia coli, Gram-positive bacterium Staphylococcus aureus and fungus Candida albicans were examined. The deposition of colloidal Ag nanoparticles onto corona pretreated polyester fabrics provides excellent antimicrobial activity and satisfactory laundering durability of obtained effects.",
journal = "Proc. 2nd Aachen-Dresden International Textile Conference, 4-5 December, 2008, Dresden, Germany",
title = "The Antimicrobial Efficiency of Corona Pretreated Polyester Fabrics Loaded with Silver Nanoparticles",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6514"
}

Šaponjic, Z., Ilić, V., Vodnik, V., Molina, R., Mihailović, D., Jovančić, P., Nedeljković, J.,& Radetić, M.. (2008). The Antimicrobial Efficiency of Corona Pretreated Polyester Fabrics Loaded with Silver Nanoparticles. in Proc. 2nd Aachen-Dresden International Textile Conference, 4-5 December, 2008, Dresden, Germany.
https://hdl.handle.net/21.15107/rcub_technorep_6514

Šaponjic Z, Ilić V, Vodnik V, Molina R, Mihailović D, Jovančić P, Nedeljković J, Radetić M. The Antimicrobial Efficiency of Corona Pretreated Polyester Fabrics Loaded with Silver Nanoparticles. in Proc. 2nd Aachen-Dresden International Textile Conference, 4-5 December, 2008, Dresden, Germany. 2008;.
https://hdl.handle.net/21.15107/rcub_technorep_6514 .

Šaponjic, Zoran, Ilić, Vesna, Vodnik, Vesna, Molina, Ricardo, Mihailović, Darka, Jovančić, Petar, Nedeljković, Jovan, Radetić, Maja, "The Antimicrobial Efficiency of Corona Pretreated Polyester Fabrics Loaded with Silver Nanoparticles" in Proc. 2nd Aachen-Dresden International Textile Conference, 4-5 December, 2008, Dresden, Germany (2008),
https://hdl.handle.net/21.15107/rcub_technorep_6514 .

TY  - CONF
AU  - Radetić, Maja
AU  - Radojević, Darinka
AU  - Ilić, Vesna
AU  - Mihailović, Darka
AU  - Jovančić, Petar
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6506
AB  - The aim of this study was investigate the possible application of recycled wool-based nonwoven material for removal of different dyes that are used in textile dyehouses. Basic, reactive, direct and metal complex dyes were investigated. The sorption kinetics, the influence of initial dye concentration, pH and temperature were analyzed. The sorption properties were highly influenced by type of the dye due to differences in their chemical structure and thus, the mechanism of binding to wool. Modification of material with chitosan and hydrogen peroxide improved the sorption capacities and sorption rates but no general trend could be established. Consequently, the sorption behavior had to be analyzed separately for each type of the dye studied. The results indicated that recycled wool-based nonwoven material can be used as an efficient, low-cost sorbent for decolorisation of effluents.
PB  - Meta Basim Press
C3  - Proceedings of the 3rd International Technical Textiles Congress, 1-2 december 2007, Istanbul, Turkey
T1  - Recycled Wool-based Nonwoven Material for Decolorisation of Dyehouse Effluents
EP  - 253
SP  - 245
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6506
ER  - 

@conference{
author = "Radetić, Maja and Radojević, Darinka and Ilić, Vesna and Mihailović, Darka and Jovančić, Petar",
year = "2007",
abstract = "The aim of this study was investigate the possible application of recycled wool-based nonwoven material for removal of different dyes that are used in textile dyehouses. Basic, reactive, direct and metal complex dyes were investigated. The sorption kinetics, the influence of initial dye concentration, pH and temperature were analyzed. The sorption properties were highly influenced by type of the dye due to differences in their chemical structure and thus, the mechanism of binding to wool. Modification of material with chitosan and hydrogen peroxide improved the sorption capacities and sorption rates but no general trend could be established. Consequently, the sorption behavior had to be analyzed separately for each type of the dye studied. The results indicated that recycled wool-based nonwoven material can be used as an efficient, low-cost sorbent for decolorisation of effluents.",
publisher = "Meta Basim Press",
journal = "Proceedings of the 3rd International Technical Textiles Congress, 1-2 december 2007, Istanbul, Turkey",
title = "Recycled Wool-based Nonwoven Material for Decolorisation of Dyehouse Effluents",
pages = "253-245",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6506"
}

Radetić, M., Radojević, D., Ilić, V., Mihailović, D.,& Jovančić, P.. (2007). Recycled Wool-based Nonwoven Material for Decolorisation of Dyehouse Effluents. in Proceedings of the 3rd International Technical Textiles Congress, 1-2 december 2007, Istanbul, Turkey
Meta Basim Press., 245-253.
https://hdl.handle.net/21.15107/rcub_technorep_6506

Radetić M, Radojević D, Ilić V, Mihailović D, Jovančić P. Recycled Wool-based Nonwoven Material for Decolorisation of Dyehouse Effluents. in Proceedings of the 3rd International Technical Textiles Congress, 1-2 december 2007, Istanbul, Turkey. 2007;:245-253.
https://hdl.handle.net/21.15107/rcub_technorep_6506 .

Radetić, Maja, Radojević, Darinka, Ilić, Vesna, Mihailović, Darka, Jovančić, Petar, "Recycled Wool-based Nonwoven Material for Decolorisation of Dyehouse Effluents" in Proceedings of the 3rd International Technical Textiles Congress, 1-2 december 2007, Istanbul, Turkey (2007):245-253,
https://hdl.handle.net/21.15107/rcub_technorep_6506 .