TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2024-03
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7319
AB  - A crucial aspect of tissue self-organization during morphogenesis, wound healing, and cancer invasion is directed migration of cell collectives. The majority of in vivo directed migration has been guided by chemotaxis, whereby cells follow a chemical gradient. In certain situations, migrating cell collectives can also self-generate the stiffness gradient in the surrounding tissue, which can have a feedback effect on the directionality of the migration. The phenomenon has been observed during collective durotaxis in vivo. Along the biointerface between neighbouring tissues, heterotypic cell-cell interactions are the main cause of this self-generated stiffness gradient. The physical processes in charge of tissue self-organization along the biointerface, which are related to the interplay between cell signalling and the formation of heterotypic cell-cell adhesion contacts, are less well-developed than the biological mechanisms of the cellular interactions. This complex phenomenon is discussed here in the model system, such as collective migration of neural crest cells between ectodermal placode and mesoderm subpopulations within Xenopus embryos by pointing to the role of the dynamics along the biointerface between adjacent cell subpopulations on the subpopulation stiffness.
PB  - Elsevier B.V.
T2  - Biosystems
T1  - Collective durotaxis along a self-generated mobile stiffness gradient in vivo
SP  - 105155
VL  - 237
DO  - 10.1016/j.biosystems.2024.105155
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2024-03",
abstract = "A crucial aspect of tissue self-organization during morphogenesis, wound healing, and cancer invasion is directed migration of cell collectives. The majority of in vivo directed migration has been guided by chemotaxis, whereby cells follow a chemical gradient. In certain situations, migrating cell collectives can also self-generate the stiffness gradient in the surrounding tissue, which can have a feedback effect on the directionality of the migration. The phenomenon has been observed during collective durotaxis in vivo. Along the biointerface between neighbouring tissues, heterotypic cell-cell interactions are the main cause of this self-generated stiffness gradient. The physical processes in charge of tissue self-organization along the biointerface, which are related to the interplay between cell signalling and the formation of heterotypic cell-cell adhesion contacts, are less well-developed than the biological mechanisms of the cellular interactions. This complex phenomenon is discussed here in the model system, such as collective migration of neural crest cells between ectodermal placode and mesoderm subpopulations within Xenopus embryos by pointing to the role of the dynamics along the biointerface between adjacent cell subpopulations on the subpopulation stiffness.",
publisher = "Elsevier B.V.",
journal = "Biosystems",
title = "Collective durotaxis along a self-generated mobile stiffness gradient in vivo",
pages = "105155",
volume = "237",
doi = "10.1016/j.biosystems.2024.105155"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2024-03). Collective durotaxis along a self-generated mobile stiffness gradient in vivo. in Biosystems
Elsevier B.V.., 237, 105155.
https://doi.org/10.1016/j.biosystems.2024.105155

Pajić-Lijaković I, Milivojević M. Collective durotaxis along a self-generated mobile stiffness gradient in vivo. in Biosystems. 2024;237:105155.
doi:10.1016/j.biosystems.2024.105155 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Collective durotaxis along a self-generated mobile stiffness gradient in vivo" in Biosystems, 237 (2024-03):105155,
https://doi.org/10.1016/j.biosystems.2024.105155 . .

TY  - CHAP
AU  - Milivojević, Milan
AU  - Pajić-Lijaković, Ivana
AU  - Dajić, Zora
AU  - Dhara, Amal Kumar
AU  - Nayak, Amit Kumar
AU  - Hasnain, Md Saquib
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7431
AB  - In many cases, conventional therapeutics are not able to cure diseases in a proper and safe manner. Conversely, plants and their derivatives have been successfully used for the treatment of numerous diseases and disorders for centuries and have proven themselves as efficient, inexpensive, environmentally friendly, faster, and less toxic. However, their use is sometimes connected to some issues, mainly due to inefficient systemic delivery and bioavailability, which prevent to translate their promising in vitro and in vivo effects into clinical use. During the past decade, nanotechnology has made big progress in developing different nanocarriers for drug delivery formulations. In the case of phytopharmaceuticals their encapsulation in nanoparticles can help in improving drug solubility, physical and chemical stability, pharmacological activity, reducing its toxicity and side effects, and providing targeted and sustained delivery which all leads to better bioavailability. The current review highlights the main problems connected to the delivery of phytopharmaceuticals, the main properties of different types of plant drugs, and gives a critical review of properties and limitations connected to the application of numerous lipid, polymer, and inorganic nanoparticles as phytodrug carriers. It also provides information about up-to-date investigated combinations of phytopharmaceuticals and nanocarriers.
PB  - Singapore : Springer
T2  - Role of Herbal Medicines
T1  - Nanotechnology in Delivery and Targeting of Phytochemicals for Lifestyle Diseases
EP  - 524
SP  - 497
DO  - 10.1007/978-981-99-7703-1_25
ER  - 

@inbook{
author = "Milivojević, Milan and Pajić-Lijaković, Ivana and Dajić, Zora and Dhara, Amal Kumar and Nayak, Amit Kumar and Hasnain, Md Saquib",
year = "2024",
abstract = "In many cases, conventional therapeutics are not able to cure diseases in a proper and safe manner. Conversely, plants and their derivatives have been successfully used for the treatment of numerous diseases and disorders for centuries and have proven themselves as efficient, inexpensive, environmentally friendly, faster, and less toxic. However, their use is sometimes connected to some issues, mainly due to inefficient systemic delivery and bioavailability, which prevent to translate their promising in vitro and in vivo effects into clinical use. During the past decade, nanotechnology has made big progress in developing different nanocarriers for drug delivery formulations. In the case of phytopharmaceuticals their encapsulation in nanoparticles can help in improving drug solubility, physical and chemical stability, pharmacological activity, reducing its toxicity and side effects, and providing targeted and sustained delivery which all leads to better bioavailability. The current review highlights the main problems connected to the delivery of phytopharmaceuticals, the main properties of different types of plant drugs, and gives a critical review of properties and limitations connected to the application of numerous lipid, polymer, and inorganic nanoparticles as phytodrug carriers. It also provides information about up-to-date investigated combinations of phytopharmaceuticals and nanocarriers.",
publisher = "Singapore : Springer",
journal = "Role of Herbal Medicines",
booktitle = "Nanotechnology in Delivery and Targeting of Phytochemicals for Lifestyle Diseases",
pages = "524-497",
doi = "10.1007/978-981-99-7703-1_25"
}

Milivojević, M., Pajić-Lijaković, I., Dajić, Z., Dhara, A. K., Nayak, A. K.,& Hasnain, M. S.. (2024). Nanotechnology in Delivery and Targeting of Phytochemicals for Lifestyle Diseases. in Role of Herbal Medicines
Singapore : Springer., 497-524.
https://doi.org/10.1007/978-981-99-7703-1_25

Milivojević M, Pajić-Lijaković I, Dajić Z, Dhara AK, Nayak AK, Hasnain MS. Nanotechnology in Delivery and Targeting of Phytochemicals for Lifestyle Diseases. in Role of Herbal Medicines. 2024;:497-524.
doi:10.1007/978-981-99-7703-1_25 .

Milivojević, Milan, Pajić-Lijaković, Ivana, Dajić, Zora, Dhara, Amal Kumar, Nayak, Amit Kumar, Hasnain, Md Saquib, "Nanotechnology in Delivery and Targeting of Phytochemicals for Lifestyle Diseases" in Role of Herbal Medicines (2024):497-524,
https://doi.org/10.1007/978-981-99-7703-1_25 . .

TY  - CHAP
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7191
AB  - Breast cancer is females’ most common cancer, with a high mortality rate primarily due to metastasis to secondary sites in the body. The migration of cancer cells from the primary tumor is influenced by physical interactions between cancer cells and surrounding epithelium and the extracellular matrix. Cumulative effects of these interactions arise in the form of physical factors such as solid stress accumulated within a tumor spheroid, tissue surface tension, and the viscoelasticity caused by collective cell migration. The cancer spreading is regulated by solid stress generated in spheroid core region during tumor growth and its interactions with external tissue. Tissue viscoelasticity and surface tension are influenced by strength of cell–cell and cell–extracellular matrix adhesion contacts, intracellular signaling cascades, viscoelasticity of extracellular matrix, and cell contractility in response to microenvironmental conditions. However, the interplay between those factors is still unclear. In order to clarify this issue, it is necessary to consider and compare the rearrangement of various mono-cultured breast cancer and epithelial model systems under in vitro conditions like rearrangement of cell spheroids and the fusion of two cell spheroids. In this chapter, focus is on the multi-scale modelling approaches aimed at reproducing and understanding these biological systems.
PB  - London : World Scientific Publishing
T2  - Modeling and Computational Approaches for Multiscale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment
T1  - Modeling and Computational Approaches for Multi-scale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment Viscoelastic Aspects of Solid Cancers
EP  - 119
SP  - 93
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7191
ER  - 

@inbook{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2024",
abstract = "Breast cancer is females’ most common cancer, with a high mortality rate primarily due to metastasis to secondary sites in the body. The migration of cancer cells from the primary tumor is influenced by physical interactions between cancer cells and surrounding epithelium and the extracellular matrix. Cumulative effects of these interactions arise in the form of physical factors such as solid stress accumulated within a tumor spheroid, tissue surface tension, and the viscoelasticity caused by collective cell migration. The cancer spreading is regulated by solid stress generated in spheroid core region during tumor growth and its interactions with external tissue. Tissue viscoelasticity and surface tension are influenced by strength of cell–cell and cell–extracellular matrix adhesion contacts, intracellular signaling cascades, viscoelasticity of extracellular matrix, and cell contractility in response to microenvironmental conditions. However, the interplay between those factors is still unclear. In order to clarify this issue, it is necessary to consider and compare the rearrangement of various mono-cultured breast cancer and epithelial model systems under in vitro conditions like rearrangement of cell spheroids and the fusion of two cell spheroids. In this chapter, focus is on the multi-scale modelling approaches aimed at reproducing and understanding these biological systems.",
publisher = "London : World Scientific Publishing",
journal = "Modeling and Computational Approaches for Multiscale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment",
booktitle = "Modeling and Computational Approaches for Multi-scale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment Viscoelastic Aspects of Solid Cancers",
pages = "119-93",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7191"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2024). Modeling and Computational Approaches for Multi-scale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment Viscoelastic Aspects of Solid Cancers. in Modeling and Computational Approaches for Multiscale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment
London : World Scientific Publishing., 93-119.
https://hdl.handle.net/21.15107/rcub_technorep_7191

Pajić-Lijaković I, Milivojević M. Modeling and Computational Approaches for Multi-scale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment Viscoelastic Aspects of Solid Cancers. in Modeling and Computational Approaches for Multiscale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment. 2024;:93-119.
https://hdl.handle.net/21.15107/rcub_technorep_7191 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Modeling and Computational Approaches for Multi-scale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment Viscoelastic Aspects of Solid Cancers" in Modeling and Computational Approaches for Multiscale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment (2024):93-119,
https://hdl.handle.net/21.15107/rcub_technorep_7191 .

TY  - JOUR
AU  - Livshits, Leonid
AU  - Peretz, Sari
AU  - Bogdanova, Anna
AU  - Zoabi, Hiba
AU  - Eitam, Harel
AU  - Barshtein, Gregory
AU  - Galindo, Cindy
AU  - Feldman, Yuri
AU  - Pajić-Lijaković, Ivana
AU  - Koren, Ariel
AU  - Gassmann, Max
AU  - Levin, Carina
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7180
AB  - The membrane-bound hemoglobin (Hb) fraction impacts red blood cell (RBC) rheology and metabolism. Therefore, Hb–RBC membrane interactions are precisely controlled. For instance, the signaling function of membrane-bound deoxy-Hb and the structure of the docking sites in the cytosolic domain of the anion exchanger 1 (AE-1) protein are well documented; however, much less is known about the interaction of Hb variants with the erythrocyte’s membrane. Here, we identified factors other than O2 availability that control Hb abundance in the membrane-bound fraction and the possible variant-specific binding selectivity of Hb to the membrane. We show that depletion of extracellular Ca2+ by chelators, or its omission from the extracellular medium, leads to membrane-bound Hb release into the cytosol. The removal of extracellular Ca2+ further triggers the redistribution of HbA0 and HbA2 variants between the membrane and the cytosol in favor of membrane-bound HbA2. Both effects are reversible and are no longer observed upon reintroduction of Ca2+ into the extracellular medium. Fluctuations of cytosolic Ca2+ also impact the pre-membrane Hb pool, resulting in the massive transfer of Hb to the cellular cytosol. We hypothesize that AE-1 is the specific membrane target and discuss the physiological outcomes and possible clinical implications of the Ca2+ regulation of the intracellular Hb distribution.
PB  - MDPI AG
T2  - Cells
T1  - The Impact of Ca2+ on Intracellular Distribution of Hemoglobin in Human Erythrocytes
IS  - 18
SP  - 2280
VL  - 12
DO  - 10.3390/cells12182280
ER  - 

@article{
author = "Livshits, Leonid and Peretz, Sari and Bogdanova, Anna and Zoabi, Hiba and Eitam, Harel and Barshtein, Gregory and Galindo, Cindy and Feldman, Yuri and Pajić-Lijaković, Ivana and Koren, Ariel and Gassmann, Max and Levin, Carina",
year = "2023",
abstract = "The membrane-bound hemoglobin (Hb) fraction impacts red blood cell (RBC) rheology and metabolism. Therefore, Hb–RBC membrane interactions are precisely controlled. For instance, the signaling function of membrane-bound deoxy-Hb and the structure of the docking sites in the cytosolic domain of the anion exchanger 1 (AE-1) protein are well documented; however, much less is known about the interaction of Hb variants with the erythrocyte’s membrane. Here, we identified factors other than O2 availability that control Hb abundance in the membrane-bound fraction and the possible variant-specific binding selectivity of Hb to the membrane. We show that depletion of extracellular Ca2+ by chelators, or its omission from the extracellular medium, leads to membrane-bound Hb release into the cytosol. The removal of extracellular Ca2+ further triggers the redistribution of HbA0 and HbA2 variants between the membrane and the cytosol in favor of membrane-bound HbA2. Both effects are reversible and are no longer observed upon reintroduction of Ca2+ into the extracellular medium. Fluctuations of cytosolic Ca2+ also impact the pre-membrane Hb pool, resulting in the massive transfer of Hb to the cellular cytosol. We hypothesize that AE-1 is the specific membrane target and discuss the physiological outcomes and possible clinical implications of the Ca2+ regulation of the intracellular Hb distribution.",
publisher = "MDPI AG",
journal = "Cells",
title = "The Impact of Ca2+ on Intracellular Distribution of Hemoglobin in Human Erythrocytes",
number = "18",
pages = "2280",
volume = "12",
doi = "10.3390/cells12182280"
}

Livshits, L., Peretz, S., Bogdanova, A., Zoabi, H., Eitam, H., Barshtein, G., Galindo, C., Feldman, Y., Pajić-Lijaković, I., Koren, A., Gassmann, M.,& Levin, C.. (2023). The Impact of Ca2+ on Intracellular Distribution of Hemoglobin in Human Erythrocytes. in Cells
MDPI AG., 12(18), 2280.
https://doi.org/10.3390/cells12182280

Livshits L, Peretz S, Bogdanova A, Zoabi H, Eitam H, Barshtein G, Galindo C, Feldman Y, Pajić-Lijaković I, Koren A, Gassmann M, Levin C. The Impact of Ca2+ on Intracellular Distribution of Hemoglobin in Human Erythrocytes. in Cells. 2023;12(18):2280.
doi:10.3390/cells12182280 .

Livshits, Leonid, Peretz, Sari, Bogdanova, Anna, Zoabi, Hiba, Eitam, Harel, Barshtein, Gregory, Galindo, Cindy, Feldman, Yuri, Pajić-Lijaković, Ivana, Koren, Ariel, Gassmann, Max, Levin, Carina, "The Impact of Ca2+ on Intracellular Distribution of Hemoglobin in Human Erythrocytes" in Cells, 12, no. 18 (2023):2280,
https://doi.org/10.3390/cells12182280 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5287
AB  - A key feature that distinguishes cancer cells from all other cells is their capability to spread throughout the body. Although how cancer cells collectively migrate by following molecular rules which influence the state of cell-cell adhesion contacts has been comprehensively formulated, the impact of physical interactions on cell spreading remains less understood. Cumulative effects of physical interactions exist as the interplay between various physical parameters such as (1) tissue surface tension, (2) viscoelasticity caused by collective cell migration, and (3) solid stress accumulated in the cell aggregate core region. This review aims to point out the role of these physical parameters in cancer cell spreading by considering and comparing the rearrangement of various mono-cultured cancer and epithelial model systems such as the fusion of two cell aggregates. While epithelial cells undergo volumetric cell rearrangement driven by the tissue surface tension, which directs cell movement from the surface to the core region of two-aggregate systems, cancer cells rather perform surface cell rearrangement. Cancer cells migrate toward the surface of the two-aggregate system driven by the solid stress while the surface tension is significantly reduced. The solid stress, accumulated in the core region of the two-aggregate system, is capable of suppressing the movement of epithelial cells that can undergo the jamming state transition; however, this stress enhances the movement of cancer cells. We have focused here on the multi-scale rheological modeling approaches that aimed at reproducing and understanding these biological systems.
PB  - Tech Science Press
T2  - Biocell
T1  - Surface activity of cancer cells: The fusion of two cell aggregates
EP  - 25
IS  - 1
SP  - 15
VL  - 47
DO  - 10.32604/biocell.2023.023469
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2023",
abstract = "A key feature that distinguishes cancer cells from all other cells is their capability to spread throughout the body. Although how cancer cells collectively migrate by following molecular rules which influence the state of cell-cell adhesion contacts has been comprehensively formulated, the impact of physical interactions on cell spreading remains less understood. Cumulative effects of physical interactions exist as the interplay between various physical parameters such as (1) tissue surface tension, (2) viscoelasticity caused by collective cell migration, and (3) solid stress accumulated in the cell aggregate core region. This review aims to point out the role of these physical parameters in cancer cell spreading by considering and comparing the rearrangement of various mono-cultured cancer and epithelial model systems such as the fusion of two cell aggregates. While epithelial cells undergo volumetric cell rearrangement driven by the tissue surface tension, which directs cell movement from the surface to the core region of two-aggregate systems, cancer cells rather perform surface cell rearrangement. Cancer cells migrate toward the surface of the two-aggregate system driven by the solid stress while the surface tension is significantly reduced. The solid stress, accumulated in the core region of the two-aggregate system, is capable of suppressing the movement of epithelial cells that can undergo the jamming state transition; however, this stress enhances the movement of cancer cells. We have focused here on the multi-scale rheological modeling approaches that aimed at reproducing and understanding these biological systems.",
publisher = "Tech Science Press",
journal = "Biocell",
title = "Surface activity of cancer cells: The fusion of two cell aggregates",
pages = "25-15",
number = "1",
volume = "47",
doi = "10.32604/biocell.2023.023469"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2023). Surface activity of cancer cells: The fusion of two cell aggregates. in Biocell
Tech Science Press., 47(1), 15-25.
https://doi.org/10.32604/biocell.2023.023469

Pajić-Lijaković I, Milivojević M. Surface activity of cancer cells: The fusion of two cell aggregates. in Biocell. 2023;47(1):15-25.
doi:10.32604/biocell.2023.023469 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Surface activity of cancer cells: The fusion of two cell aggregates" in Biocell, 47, no. 1 (2023):15-25,
https://doi.org/10.32604/biocell.2023.023469 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5300
AB  - Morphogenesis, tissue regeneration, and cancer invasion involve transitions in tissue morphology. These transitions, caused by collective cell migration (CCM), have been interpreted as active wetting/de-wetting transitions. This phenomenon is considered based on a model system as wetting of a cell aggregate on a rigid substrate, which includes cell aggregate movement and isotropic/anisotropic spreading of a cell monolayer around the aggregate depending on the substrate rigidity and aggregate size. This model system accounts for the transition between 3D epithelial aggregate and 2D cell monolayer as a product of: (1) tissue surface tension, (2) surface tension of substrate matrix, (3) cell–matrix interfacial tension, (4) interfacial tension gradient, (5) viscoelasticity caused by CCM, and (6) viscoelasticity of substrate matrix. These physical parameters depend on the cell contractility and state of cell–cell and cell–matrix adhesion contacts, as well as the stretching/compression of cellular systems caused by CCM. Despite extensive research devoted to study cell wetting, we still do not understand the interplay among these physical parameters which induces an oscillatory trend of cell rearrangement. This review focuses on these physical parameters in governing the cell rearrangement in the context of epithelial aggregate wetting/de-wetting, and on modeling approaches aimed at reproducing and understanding these biological systems. In this context, we not only review previously published biophysical models for cell rearrangement caused by CCM, but also propose new extensions of those models to point out the interrelation between cell–matrix interfacial tension and epithelial viscoelasticity and the role of the interfacial tension gradient in cell spreading.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - European Biophysics Journal
T1  - Active wetting of epithelial tissues: modeling considerations
EP  - 15
IS  - 1-2
SP  - 1
VL  - 52
DO  - 10.1007/s00249-022-01625-w
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2023",
abstract = "Morphogenesis, tissue regeneration, and cancer invasion involve transitions in tissue morphology. These transitions, caused by collective cell migration (CCM), have been interpreted as active wetting/de-wetting transitions. This phenomenon is considered based on a model system as wetting of a cell aggregate on a rigid substrate, which includes cell aggregate movement and isotropic/anisotropic spreading of a cell monolayer around the aggregate depending on the substrate rigidity and aggregate size. This model system accounts for the transition between 3D epithelial aggregate and 2D cell monolayer as a product of: (1) tissue surface tension, (2) surface tension of substrate matrix, (3) cell–matrix interfacial tension, (4) interfacial tension gradient, (5) viscoelasticity caused by CCM, and (6) viscoelasticity of substrate matrix. These physical parameters depend on the cell contractility and state of cell–cell and cell–matrix adhesion contacts, as well as the stretching/compression of cellular systems caused by CCM. Despite extensive research devoted to study cell wetting, we still do not understand the interplay among these physical parameters which induces an oscillatory trend of cell rearrangement. This review focuses on these physical parameters in governing the cell rearrangement in the context of epithelial aggregate wetting/de-wetting, and on modeling approaches aimed at reproducing and understanding these biological systems. In this context, we not only review previously published biophysical models for cell rearrangement caused by CCM, but also propose new extensions of those models to point out the interrelation between cell–matrix interfacial tension and epithelial viscoelasticity and the role of the interfacial tension gradient in cell spreading.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "European Biophysics Journal",
title = "Active wetting of epithelial tissues: modeling considerations",
pages = "15-1",
number = "1-2",
volume = "52",
doi = "10.1007/s00249-022-01625-w"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2023). Active wetting of epithelial tissues: modeling considerations. in European Biophysics Journal
Springer Science and Business Media Deutschland GmbH., 52(1-2), 1-15.
https://doi.org/10.1007/s00249-022-01625-w

Pajić-Lijaković I, Milivojević M. Active wetting of epithelial tissues: modeling considerations. in European Biophysics Journal. 2023;52(1-2):1-15.
doi:10.1007/s00249-022-01625-w .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Active wetting of epithelial tissues: modeling considerations" in European Biophysics Journal, 52, no. 1-2 (2023):1-15,
https://doi.org/10.1007/s00249-022-01625-w . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6582
AB  - Epithelial cancers are the most common cancer diseases worldwide within last few years. Many of these diseases can be cured if treated effectively in an early stage. The early stage of diseases includes the spreading of cancer cells through healthy epithelium. This spreading of cancer (mesenchymal) cells influences the reorganization of epithelium itself via collective cell migration, which has a feedback to heterotypic interactions along the bio-interface between epithelial and cancer subpopulations and further cancer spreading. This cause–consequence inter-relation is guided by the interplay among biological and physical factors. While biological factors, such as cell signaling and gene expression, which influence cell contractility and remodeling of cell–cell and cell–matrix adhesion contacts and consequently, homotopic and heterotypic interactions are well elaborated, we still don’t understand properly the impact of physical factors on altered morphological changes of epithelial cells. The deeper insight into these physical interactions may help us to solve some long-standing questions in disease progression and can lead to advance in cancer diagnostics and therapies. This review focuses on the role of the physical factors, such as epithelial and cancer surface tensions, interfacial tension between the subpopulations, gradients of interfacial tension of the subpopulations, cell residual stress generation, and frictional effects along the bio-interface on the morphological changes of epithelial cells and the impact of these changes on further spreading of cancer.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Applied Physics A: Materials Science and Processing
T1  - Morphological changes of epithelial cells and spreading of cancer: theoretical consideration
IS  - 8
SP  - 553
VL  - 129
DO  - 10.1007/s00339-023-06814-8
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2023",
abstract = "Epithelial cancers are the most common cancer diseases worldwide within last few years. Many of these diseases can be cured if treated effectively in an early stage. The early stage of diseases includes the spreading of cancer cells through healthy epithelium. This spreading of cancer (mesenchymal) cells influences the reorganization of epithelium itself via collective cell migration, which has a feedback to heterotypic interactions along the bio-interface between epithelial and cancer subpopulations and further cancer spreading. This cause–consequence inter-relation is guided by the interplay among biological and physical factors. While biological factors, such as cell signaling and gene expression, which influence cell contractility and remodeling of cell–cell and cell–matrix adhesion contacts and consequently, homotopic and heterotypic interactions are well elaborated, we still don’t understand properly the impact of physical factors on altered morphological changes of epithelial cells. The deeper insight into these physical interactions may help us to solve some long-standing questions in disease progression and can lead to advance in cancer diagnostics and therapies. This review focuses on the role of the physical factors, such as epithelial and cancer surface tensions, interfacial tension between the subpopulations, gradients of interfacial tension of the subpopulations, cell residual stress generation, and frictional effects along the bio-interface on the morphological changes of epithelial cells and the impact of these changes on further spreading of cancer.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Applied Physics A: Materials Science and Processing",
title = "Morphological changes of epithelial cells and spreading of cancer: theoretical consideration",
number = "8",
pages = "553",
volume = "129",
doi = "10.1007/s00339-023-06814-8"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2023). Morphological changes of epithelial cells and spreading of cancer: theoretical consideration. in Applied Physics A: Materials Science and Processing
Springer Science and Business Media Deutschland GmbH., 129(8), 553.
https://doi.org/10.1007/s00339-023-06814-8

Pajić-Lijaković I, Milivojević M. Morphological changes of epithelial cells and spreading of cancer: theoretical consideration. in Applied Physics A: Materials Science and Processing. 2023;129(8):553.
doi:10.1007/s00339-023-06814-8 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Morphological changes of epithelial cells and spreading of cancer: theoretical consideration" in Applied Physics A: Materials Science and Processing, 129, no. 8 (2023):553,
https://doi.org/10.1007/s00339-023-06814-8 . .

TY  - JOUR
AU  - Milivojević, Milan
AU  - Popović, Aleksandra
AU  - Pajić-Lijaković, Ivana
AU  - Šoštarić, Ivan
AU  - Kolašinac, Stefan
AU  - Dajić Stevanović, Zora
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6616
AB  - Sodium alginate is one of the most interesting and the most investigated and applied biopolymers due to its advantageous properties. Among them, easy, simple, mild, rapid, non-toxic gelation by divalent cations is the most important. In addition, it is abundant, low-cost, eco-friendly, bio-compatible, bio-adhesive, biodegradable, stable, etc. All those properties were systematically considered within this review. Carotenoids are functional components in the human diet with plenty of health benefits. However, their sensitivity to environmental and process stresses, chemical instability, easy oxidation, low water solubility, and bioavailability limit their food and pharmaceutical applications. Encapsulation may help in overcoming these limitations and within this review, the role of alginate-based encapsulation systems in improving the stability and bioavailability of carotenoids is explored. It may be concluded that all alginate-based systems increase carotenoid stability, but only those of micro- and nano-size, as well as emulsion-based, may improve their low bioaccessibility. In addition, the incorporation of other biopolymers may further improve encapsulation system properties. Furthermore, the main techniques for evaluating the encapsulation are briefly considered. This review critically and profoundly explains the role of alginates in improving the encapsulation process of carotenoids, suggesting the best alternatives for those systems. Moreover, it provides a comprehensive cover of recent advances in this field.
PB  - MDPI
T2  - Gels
T1  - Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications
IS  - 8
SP  - 620
VL  - 9
DO  - 10.3390/gels9080620
ER  - 

@article{
author = "Milivojević, Milan and Popović, Aleksandra and Pajić-Lijaković, Ivana and Šoštarić, Ivan and Kolašinac, Stefan and Dajić Stevanović, Zora",
year = "2023",
abstract = "Sodium alginate is one of the most interesting and the most investigated and applied biopolymers due to its advantageous properties. Among them, easy, simple, mild, rapid, non-toxic gelation by divalent cations is the most important. In addition, it is abundant, low-cost, eco-friendly, bio-compatible, bio-adhesive, biodegradable, stable, etc. All those properties were systematically considered within this review. Carotenoids are functional components in the human diet with plenty of health benefits. However, their sensitivity to environmental and process stresses, chemical instability, easy oxidation, low water solubility, and bioavailability limit their food and pharmaceutical applications. Encapsulation may help in overcoming these limitations and within this review, the role of alginate-based encapsulation systems in improving the stability and bioavailability of carotenoids is explored. It may be concluded that all alginate-based systems increase carotenoid stability, but only those of micro- and nano-size, as well as emulsion-based, may improve their low bioaccessibility. In addition, the incorporation of other biopolymers may further improve encapsulation system properties. Furthermore, the main techniques for evaluating the encapsulation are briefly considered. This review critically and profoundly explains the role of alginates in improving the encapsulation process of carotenoids, suggesting the best alternatives for those systems. Moreover, it provides a comprehensive cover of recent advances in this field.",
publisher = "MDPI",
journal = "Gels",
title = "Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications",
number = "8",
pages = "620",
volume = "9",
doi = "10.3390/gels9080620"
}

Milivojević, M., Popović, A., Pajić-Lijaković, I., Šoštarić, I., Kolašinac, S.,& Dajić Stevanović, Z.. (2023). Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications. in Gels
MDPI., 9(8), 620.
https://doi.org/10.3390/gels9080620

Milivojević M, Popović A, Pajić-Lijaković I, Šoštarić I, Kolašinac S, Dajić Stevanović Z. Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications. in Gels. 2023;9(8):620.
doi:10.3390/gels9080620 .

Milivojević, Milan, Popović, Aleksandra, Pajić-Lijaković, Ivana, Šoštarić, Ivan, Kolašinac, Stefan, Dajić Stevanović, Zora, "Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications" in Gels, 9, no. 8 (2023):620,
https://doi.org/10.3390/gels9080620 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6637
AB  - Movement of cell clusters along extracellular matrices (ECM) during tissue development, wound healing, and early stage of cancer invasion involve various inter-connected migration modes such as: (1) cell movement within clusters, (2) cluster extension (wetting) and compression (de-wetting), and (3) directional cluster movement. It has become increasingly evident that dilational and volumetric viscoelasticity of cell clusters and their surrounding substrate significantly influence these migration modes through physical parameters such as: tissue and matrix surface tensions, interfacial tension between cells and substrate, gradients of surface and interfacial tensions, as well as, the accumulation of cell and matrix residual stresses. Inhomogeneous distribution of tissue surface tension along the cell–matrix biointerface can appear as a consequence of different contractility of various cluster regions. While the directional cell migration caused by the matrix stiffness gradient (i.e., durotaxis) has been widely elaborated, the structural changes of matrix surface caused by cell tractions which lead to the generation of the matrix surface tension gradient has not been considered yet. The main goal of this theoretical consideration is to clarify the roles of various physical parameters in collective cell migration based on the formulation of a biophysical model. This complex phenomenon is discussed with the help of model systems such as the movement of cell clusters on a collagen I gel matrix, simultaneously reviewing various experimental data with and without cells.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - European Biophysics Journal
T1  - Physics of collective cell migration
DO  - 10.1007/s00249-023-01681-w
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2023",
abstract = "Movement of cell clusters along extracellular matrices (ECM) during tissue development, wound healing, and early stage of cancer invasion involve various inter-connected migration modes such as: (1) cell movement within clusters, (2) cluster extension (wetting) and compression (de-wetting), and (3) directional cluster movement. It has become increasingly evident that dilational and volumetric viscoelasticity of cell clusters and their surrounding substrate significantly influence these migration modes through physical parameters such as: tissue and matrix surface tensions, interfacial tension between cells and substrate, gradients of surface and interfacial tensions, as well as, the accumulation of cell and matrix residual stresses. Inhomogeneous distribution of tissue surface tension along the cell–matrix biointerface can appear as a consequence of different contractility of various cluster regions. While the directional cell migration caused by the matrix stiffness gradient (i.e., durotaxis) has been widely elaborated, the structural changes of matrix surface caused by cell tractions which lead to the generation of the matrix surface tension gradient has not been considered yet. The main goal of this theoretical consideration is to clarify the roles of various physical parameters in collective cell migration based on the formulation of a biophysical model. This complex phenomenon is discussed with the help of model systems such as the movement of cell clusters on a collagen I gel matrix, simultaneously reviewing various experimental data with and without cells.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "European Biophysics Journal",
title = "Physics of collective cell migration",
doi = "10.1007/s00249-023-01681-w"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2023). Physics of collective cell migration. in European Biophysics Journal
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s00249-023-01681-w

Pajić-Lijaković I, Milivojević M. Physics of collective cell migration. in European Biophysics Journal. 2023;.
doi:10.1007/s00249-023-01681-w .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Physics of collective cell migration" in European Biophysics Journal (2023),
https://doi.org/10.1007/s00249-023-01681-w . .

TY  - JOUR
AU  - Espina, Jaime A.
AU  - Cordeiro, Marilia H.
AU  - Milivojević, Milan
AU  - Pajić-Lijaković, Ivana
AU  - Barriga, Elias H.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6662
AB  - Shear stress is essential for normal physiology and malignancy. Common physiological processes - such as blood flow, particle flow in the gut, or contact between migratory cell clusters and their substrate - produce shear stress that can have an impact on the behavior of different tissues. In addition, shear stress has roles in processes of biomedical interest, such as wound healing, cancer and fibrosis induced by soft implants. Thus, understanding how cells react and adapt to shear stress is important. In this Review, we discuss in vivo and in vitro data obtained from vascular and epithelial models; highlight the insights these have afforded regarding the general mechanisms through which cells sense, transduce and respond to shear stress at the cellular levels; and outline how the changes cells experience in response to shear stress impact tissue organization. Finally, we discuss the role of shear stress in collective cell migration, which is only starting to be appreciated. We review our current understanding of the effects of shear stress in the context of embryo development, cancer and fibrosis, and invite the scientific community to further investigate the role of shear stress in these scenarios.
PB  - Company of Biologists Ltd.
T2  - Journal of Cell Science
T1  - Response of cells and tissues to shear stress
IS  - 18
SP  - jcs260985
VL  - 136
DO  - 10.1242/jcs.260985
ER  - 

@article{
author = "Espina, Jaime A. and Cordeiro, Marilia H. and Milivojević, Milan and Pajić-Lijaković, Ivana and Barriga, Elias H.",
year = "2023",
abstract = "Shear stress is essential for normal physiology and malignancy. Common physiological processes - such as blood flow, particle flow in the gut, or contact between migratory cell clusters and their substrate - produce shear stress that can have an impact on the behavior of different tissues. In addition, shear stress has roles in processes of biomedical interest, such as wound healing, cancer and fibrosis induced by soft implants. Thus, understanding how cells react and adapt to shear stress is important. In this Review, we discuss in vivo and in vitro data obtained from vascular and epithelial models; highlight the insights these have afforded regarding the general mechanisms through which cells sense, transduce and respond to shear stress at the cellular levels; and outline how the changes cells experience in response to shear stress impact tissue organization. Finally, we discuss the role of shear stress in collective cell migration, which is only starting to be appreciated. We review our current understanding of the effects of shear stress in the context of embryo development, cancer and fibrosis, and invite the scientific community to further investigate the role of shear stress in these scenarios.",
publisher = "Company of Biologists Ltd.",
journal = "Journal of Cell Science",
title = "Response of cells and tissues to shear stress",
number = "18",
pages = "jcs260985",
volume = "136",
doi = "10.1242/jcs.260985"
}

Espina, J. A., Cordeiro, M. H., Milivojević, M., Pajić-Lijaković, I.,& Barriga, E. H.. (2023). Response of cells and tissues to shear stress. in Journal of Cell Science
Company of Biologists Ltd.., 136(18), jcs260985.
https://doi.org/10.1242/jcs.260985

Espina JA, Cordeiro MH, Milivojević M, Pajić-Lijaković I, Barriga EH. Response of cells and tissues to shear stress. in Journal of Cell Science. 2023;136(18):jcs260985.
doi:10.1242/jcs.260985 .

Espina, Jaime A., Cordeiro, Marilia H., Milivojević, Milan, Pajić-Lijaković, Ivana, Barriga, Elias H., "Response of cells and tissues to shear stress" in Journal of Cell Science, 136, no. 18 (2023):jcs260985,
https://doi.org/10.1242/jcs.260985 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6711
AB  - Collective cell migration is essential for a wide range of biological processes such as: morphogenesis, wound healing, and cancer spreading. However, it is well known that migrating epithelial collectives frequently undergo jamming, stay trapped some period of time, and then start migration again. Consequently, only a part of epithelial cells actively contributes to the tissue development. In contrast to epithelial cells, migrating mesenchymal collectives successfully avoid the jamming. It has been confirmed that the epithelial unjamming cannot be treated as the epithelial-to-mesenchymal transition. Some other mechanism is responsible for the epithelial jamming/unjamming. Despite extensive research devoted to study the cell jamming/unjamming, we still do not understand the origin of this phenomenon. The origin is connected to physical factors such as: the cell compressive residual stress accumulation and surface characteristics of migrating (unjamming) and resting (jamming) epithelial clusters which depend primarily on the strength of cell-cell adhesion contacts and cell contractility. The main goal of this theoretical consideration is to clarify these cause-consequence relations.
PB  - Elsevier Ltd.
T2  - BioSystems
T1  - Cell jamming-to-unjamming transitions and vice versa in development: Physical aspects
SP  - 105045
VL  - 234
DO  - 10.1016/j.biosystems.2023.105045
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2023",
abstract = "Collective cell migration is essential for a wide range of biological processes such as: morphogenesis, wound healing, and cancer spreading. However, it is well known that migrating epithelial collectives frequently undergo jamming, stay trapped some period of time, and then start migration again. Consequently, only a part of epithelial cells actively contributes to the tissue development. In contrast to epithelial cells, migrating mesenchymal collectives successfully avoid the jamming. It has been confirmed that the epithelial unjamming cannot be treated as the epithelial-to-mesenchymal transition. Some other mechanism is responsible for the epithelial jamming/unjamming. Despite extensive research devoted to study the cell jamming/unjamming, we still do not understand the origin of this phenomenon. The origin is connected to physical factors such as: the cell compressive residual stress accumulation and surface characteristics of migrating (unjamming) and resting (jamming) epithelial clusters which depend primarily on the strength of cell-cell adhesion contacts and cell contractility. The main goal of this theoretical consideration is to clarify these cause-consequence relations.",
publisher = "Elsevier Ltd.",
journal = "BioSystems",
title = "Cell jamming-to-unjamming transitions and vice versa in development: Physical aspects",
pages = "105045",
volume = "234",
doi = "10.1016/j.biosystems.2023.105045"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2023). Cell jamming-to-unjamming transitions and vice versa in development: Physical aspects. in BioSystems
Elsevier Ltd.., 234, 105045.
https://doi.org/10.1016/j.biosystems.2023.105045

Pajić-Lijaković I, Milivojević M. Cell jamming-to-unjamming transitions and vice versa in development: Physical aspects. in BioSystems. 2023;234:105045.
doi:10.1016/j.biosystems.2023.105045 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Cell jamming-to-unjamming transitions and vice versa in development: Physical aspects" in BioSystems, 234 (2023):105045,
https://doi.org/10.1016/j.biosystems.2023.105045 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7042
AB  - The biointerface dynamics influence any cancer spreading through the epithelium since it is documented in the early stages some malignancies (like epithelial cancer). The altered rearrangement of epithelial cells has an impact on the development of cancer. Therefore, it is necessary to comprehend the underlying biological and physical mechanisms of this biointerface dynamics for early suppression of cancer. While the biological mechanisms include cell signaling and gene expression, the physical mechanisms are several physical parameters such as the epithelial-cancer interfacial tension, epithelial surface tension, and compressive stress accumulated within the epithelium. Although the segregation of epithelia-cancer co-cultured systems was widely investigated, the role of these physical parameters in cell reorganization is still not fully recognized. Hence, this review is focused on clarifying the role that some physical parameters have during cell reorganization within the epithelial cell clusters and cancer spread within co-cultured spheroids. We have applied the developed biophysical model to point out the inter-relations among physical parameters that influence cell reorganization within epithelial-cancer co-cultured systems. The main results of this theoretical consideration have been assessed by integrating the biophysical model with biological and bio-mechanical experiments from the available literature. The epithelial-cancer interfacial tension leads to the reduction of the biointerface area, which leads to an increase in the compressive residual stress within the epithelial clusters depending on the viscoelasticity of the epithelial subpopulation. This stress impacts epithelial rearrangement and the dynamics along the biointerface by influencing the epithelial surface tension and epithelial-cancer interfacial tension. Further, the interrelation between the epithelial surface tension and epithelial-cancer interfacial tension influences the spread of cancer cells.
PB  - Tech Science Press
T2  - Biocell
T1  - Dynamics along the epithelial-cancer biointerface: Hidden system complexities
EP  - 2334
IS  - 11
SP  - 2321
VL  - 47
DO  - 10.32604/biocell.2023.043796
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2023",
abstract = "The biointerface dynamics influence any cancer spreading through the epithelium since it is documented in the early stages some malignancies (like epithelial cancer). The altered rearrangement of epithelial cells has an impact on the development of cancer. Therefore, it is necessary to comprehend the underlying biological and physical mechanisms of this biointerface dynamics for early suppression of cancer. While the biological mechanisms include cell signaling and gene expression, the physical mechanisms are several physical parameters such as the epithelial-cancer interfacial tension, epithelial surface tension, and compressive stress accumulated within the epithelium. Although the segregation of epithelia-cancer co-cultured systems was widely investigated, the role of these physical parameters in cell reorganization is still not fully recognized. Hence, this review is focused on clarifying the role that some physical parameters have during cell reorganization within the epithelial cell clusters and cancer spread within co-cultured spheroids. We have applied the developed biophysical model to point out the inter-relations among physical parameters that influence cell reorganization within epithelial-cancer co-cultured systems. The main results of this theoretical consideration have been assessed by integrating the biophysical model with biological and bio-mechanical experiments from the available literature. The epithelial-cancer interfacial tension leads to the reduction of the biointerface area, which leads to an increase in the compressive residual stress within the epithelial clusters depending on the viscoelasticity of the epithelial subpopulation. This stress impacts epithelial rearrangement and the dynamics along the biointerface by influencing the epithelial surface tension and epithelial-cancer interfacial tension. Further, the interrelation between the epithelial surface tension and epithelial-cancer interfacial tension influences the spread of cancer cells.",
publisher = "Tech Science Press",
journal = "Biocell",
title = "Dynamics along the epithelial-cancer biointerface: Hidden system complexities",
pages = "2334-2321",
number = "11",
volume = "47",
doi = "10.32604/biocell.2023.043796"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2023). Dynamics along the epithelial-cancer biointerface: Hidden system complexities. in Biocell
Tech Science Press., 47(11), 2321-2334.
https://doi.org/10.32604/biocell.2023.043796

Pajić-Lijaković I, Milivojević M. Dynamics along the epithelial-cancer biointerface: Hidden system complexities. in Biocell. 2023;47(11):2321-2334.
doi:10.32604/biocell.2023.043796 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Dynamics along the epithelial-cancer biointerface: Hidden system complexities" in Biocell, 47, no. 11 (2023):2321-2334,
https://doi.org/10.32604/biocell.2023.043796 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5164
AB  - Cells are very sensitive to the shear stress (SS). However, undesirable SS is generated during physiological process such as collective cell migration (CCM) and influences the biological processes such as morphogenesis, wound healing and cancer invasion. Despite extensive research devoted to study the SS generation caused by CCM, we still do not fully understand the main cause of SS appearance. An attempt is made here to offer some answers to these questions by considering the rearrangement of cell monolayers. The SS generation represents a consequence of natural and forced convection. While forced convection is dependent on cell speed, the natural convection is induced by the gradient of tissue surface tension. The phenomenon is known as the Marangoni effect. The gradient of tissue surface tension induces directed cell spreading from the regions of lower tissue surface tension to the regions of higher tissue surface tension and leads to the cell sorting. This directional cell migration is described by the Marangoni flux. The phenomenon has been recognized during the rearrangement of (1) epithelial cell monolayers and (2) mixed cell monolayers made by epithelial and mesenchymal cells. The consequence of the Marangoni effect is an intensive spreading of cancer cells through an epithelium. In this work, a review of existing literature about SS generation caused by CCM is given along with the assortment of published experimental findings, to invite experimentalists to test given theoretical considerations in multicellular systems.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - European Biophysics Journal
T1  - Marangoni effect and cell spreading
EP  - 429
IS  - 6
SP  - 419
VL  - 51
DO  - 10.1007/s00249-022-01612-1
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2022",
abstract = "Cells are very sensitive to the shear stress (SS). However, undesirable SS is generated during physiological process such as collective cell migration (CCM) and influences the biological processes such as morphogenesis, wound healing and cancer invasion. Despite extensive research devoted to study the SS generation caused by CCM, we still do not fully understand the main cause of SS appearance. An attempt is made here to offer some answers to these questions by considering the rearrangement of cell monolayers. The SS generation represents a consequence of natural and forced convection. While forced convection is dependent on cell speed, the natural convection is induced by the gradient of tissue surface tension. The phenomenon is known as the Marangoni effect. The gradient of tissue surface tension induces directed cell spreading from the regions of lower tissue surface tension to the regions of higher tissue surface tension and leads to the cell sorting. This directional cell migration is described by the Marangoni flux. The phenomenon has been recognized during the rearrangement of (1) epithelial cell monolayers and (2) mixed cell monolayers made by epithelial and mesenchymal cells. The consequence of the Marangoni effect is an intensive spreading of cancer cells through an epithelium. In this work, a review of existing literature about SS generation caused by CCM is given along with the assortment of published experimental findings, to invite experimentalists to test given theoretical considerations in multicellular systems.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "European Biophysics Journal",
title = "Marangoni effect and cell spreading",
pages = "429-419",
number = "6",
volume = "51",
doi = "10.1007/s00249-022-01612-1"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2022). Marangoni effect and cell spreading. in European Biophysics Journal
Springer Science and Business Media Deutschland GmbH., 51(6), 419-429.
https://doi.org/10.1007/s00249-022-01612-1

Pajić-Lijaković I, Milivojević M. Marangoni effect and cell spreading. in European Biophysics Journal. 2022;51(6):419-429.
doi:10.1007/s00249-022-01612-1 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Marangoni effect and cell spreading" in European Biophysics Journal, 51, no. 6 (2022):419-429,
https://doi.org/10.1007/s00249-022-01612-1 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7170
AB  - Although collective cell migration (CCM) is a highly coordinated and fine-tuned migratory mode, instabilities in the form of cell swirling motion (CSM) often occur. The CSM represents a product of the active turbulence obtained at low Reynolds number which has a feedback impact on various processes such as: morphogenesis, wound healing, and cancer invasion. The cause of this phenomenon is related to the viscoelasticity of multicellular systems in the context of cell residual stress accumulation. Particular interest of this work is to: (1) consider the tissue cohesiveness as the main parameter responsible for the CSM appearance, (2) discuss the viscoelasticity of multicellular systems caused by CCM by clarifying the roles of cell shear and normal residual stresses, and (3) describe the dynamics of CSM in the context of mechanical waves generation based on multiscale modeling consideration. While the cell normal residual stress induces an increase in cell packing density capable of reducing the tissue cohesiveness of healthy epithelium, the shear residual stress exerts work via shear stress torque against the tissue surface tension and can induce the CSM. Inhomogeneous distribution of the cell residual stress within the swirl leads to the generation of viscoelastic force capable of suppressing the CCM. This force together with the surface tension force acts against the centrifugal force and induces the swirl radial pulsations connected with successive stiffening and softening. In this work, a review of existing literature about viscoelasticity caused by CCM is given along with assortment of published experimental findings, in order to invite experimentalists to test given theoretical considerations in multicellular systems.
PB  - Academic Press Inc.
T2  - Advances in Applied Mechanics
T1  - Viscoelasticity and cell swirling motion
EP  - 425
SP  - 393
VL  - 55
DO  - 10.1016/bs.aams.2022.05.002
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2022",
abstract = "Although collective cell migration (CCM) is a highly coordinated and fine-tuned migratory mode, instabilities in the form of cell swirling motion (CSM) often occur. The CSM represents a product of the active turbulence obtained at low Reynolds number which has a feedback impact on various processes such as: morphogenesis, wound healing, and cancer invasion. The cause of this phenomenon is related to the viscoelasticity of multicellular systems in the context of cell residual stress accumulation. Particular interest of this work is to: (1) consider the tissue cohesiveness as the main parameter responsible for the CSM appearance, (2) discuss the viscoelasticity of multicellular systems caused by CCM by clarifying the roles of cell shear and normal residual stresses, and (3) describe the dynamics of CSM in the context of mechanical waves generation based on multiscale modeling consideration. While the cell normal residual stress induces an increase in cell packing density capable of reducing the tissue cohesiveness of healthy epithelium, the shear residual stress exerts work via shear stress torque against the tissue surface tension and can induce the CSM. Inhomogeneous distribution of the cell residual stress within the swirl leads to the generation of viscoelastic force capable of suppressing the CCM. This force together with the surface tension force acts against the centrifugal force and induces the swirl radial pulsations connected with successive stiffening and softening. In this work, a review of existing literature about viscoelasticity caused by CCM is given along with assortment of published experimental findings, in order to invite experimentalists to test given theoretical considerations in multicellular systems.",
publisher = "Academic Press Inc.",
journal = "Advances in Applied Mechanics",
title = "Viscoelasticity and cell swirling motion",
pages = "425-393",
volume = "55",
doi = "10.1016/bs.aams.2022.05.002"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2022). Viscoelasticity and cell swirling motion. in Advances in Applied Mechanics
Academic Press Inc.., 55, 393-425.
https://doi.org/10.1016/bs.aams.2022.05.002

Pajić-Lijaković I, Milivojević M. Viscoelasticity and cell swirling motion. in Advances in Applied Mechanics. 2022;55:393-425.
doi:10.1016/bs.aams.2022.05.002 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Viscoelasticity and cell swirling motion" in Advances in Applied Mechanics, 55 (2022):393-425,
https://doi.org/10.1016/bs.aams.2022.05.002 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5012
AB  - Long-timescale viscoelasticity caused by collective cell migration (CCM) significantly influences cell rearrangement and induces generation of mechanical waves. The phenomenon represents a product of the active turbulence occurring at low Reynolds number. The generation of mechanical waves has been a subject of intensive research primarily in 2D multicellular systems, while 3D systems have not been considered in this context. The aim of this contribution is to discuss the generation of mechanical waves during 3D CCM in two model systems: (1) the fusion of two-cell aggregates and (2) cell aggregate rounding after uni-axial compression, pointing out that mechanical waves represent a characteristic of CCM in general. Such perturbations are also involved in various biological processes, such as embryogenesis, wound healing and cancer invasion. The inter-relation between the viscoelasticity and the appearance of active turbulence remains poorly understood even in 2D. The phenomenon represents a consequence of the competition between the viscoelastic force and the surface tension force which induces successive stiffening and softening of parts of multicellular systems. The viscoelastic force is a product of the residual cell stress accumulation and its inhomogeneous distribution caused by CCM. This modeling consideration represents a powerful tool to address the generation of mechanical waves in CCM towards an understanding of this important but still controversial topic.
T2  - European Biophysics Journal With Biophysics Letters
T1  - Mechanical waves caused by collective cell migration: generation
EP  - 13
IS  - 1
SP  - 1
VL  - 51
DO  - 10.1007/s00249-021-01581-x
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2022",
abstract = "Long-timescale viscoelasticity caused by collective cell migration (CCM) significantly influences cell rearrangement and induces generation of mechanical waves. The phenomenon represents a product of the active turbulence occurring at low Reynolds number. The generation of mechanical waves has been a subject of intensive research primarily in 2D multicellular systems, while 3D systems have not been considered in this context. The aim of this contribution is to discuss the generation of mechanical waves during 3D CCM in two model systems: (1) the fusion of two-cell aggregates and (2) cell aggregate rounding after uni-axial compression, pointing out that mechanical waves represent a characteristic of CCM in general. Such perturbations are also involved in various biological processes, such as embryogenesis, wound healing and cancer invasion. The inter-relation between the viscoelasticity and the appearance of active turbulence remains poorly understood even in 2D. The phenomenon represents a consequence of the competition between the viscoelastic force and the surface tension force which induces successive stiffening and softening of parts of multicellular systems. The viscoelastic force is a product of the residual cell stress accumulation and its inhomogeneous distribution caused by CCM. This modeling consideration represents a powerful tool to address the generation of mechanical waves in CCM towards an understanding of this important but still controversial topic.",
journal = "European Biophysics Journal With Biophysics Letters",
title = "Mechanical waves caused by collective cell migration: generation",
pages = "13-1",
number = "1",
volume = "51",
doi = "10.1007/s00249-021-01581-x"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2022). Mechanical waves caused by collective cell migration: generation. in European Biophysics Journal With Biophysics Letters, 51(1), 1-13.
https://doi.org/10.1007/s00249-021-01581-x

Pajić-Lijaković I, Milivojević M. Mechanical waves caused by collective cell migration: generation. in European Biophysics Journal With Biophysics Letters. 2022;51(1):1-13.
doi:10.1007/s00249-021-01581-x .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Mechanical waves caused by collective cell migration: generation" in European Biophysics Journal With Biophysics Letters, 51, no. 1 (2022):1-13,
https://doi.org/10.1007/s00249-021-01581-x . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
AU  - Clark, Andrew G.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5185
AB  - Collective cell migration on extracellular matrix (ECM) networks is a key biological process involved in development, tissue homeostasis and diseases such as metastatic cancer. During invasion of epithelial cancers, cell clusters migrate through the surrounding stroma, which is comprised primarily of networks of collagen-I fibers. There is growing evidence that the rheological and topological properties of collagen networks can impact cell behavior and cell migration dynamics. During migration, cells exert mechanical forces on their substrate, resulting in an active remodeling of ECM networks that depends not only on the forces produced, but also on the molecular mechanisms that dictate network rheology. One aspect of collagen network rheology whose role is emerging as a crucial parameter in dictating cell behavior is network viscoelasticity. Dynamic reorganization of ECM networks can induce local changes in network organization and mechanics, which can further feed back on cell migration dynamics and cell-cell rearrangement. A number of studies, including many recent publications, have investigated the mechanisms underlying structural changes to collagen networks in response to mechanical force as well as the role of collagen rheology and topology in regulating cell behavior. In this mini-review, we explore the cause-consequence relationship between collagen network viscoelasticity and cell rearrangements at various spatiotemporal scales. We focus on structural alterations of collagen-I networks during collective cell migration and discuss the main rheological parameters, and in particular the role of viscoelasticity, which can contribute to local matrix stiffening during cell movement and can elicit changes in cell dynamics.
PB  - Frontiers Media S.A.
T2  - Frontiers in Cell and Developmental Biology
T1  - Collective Cell Migration on Collagen-I Networks: The Impact of Matrix Viscoelasticity
EP  - 901026
VL  - 10
DO  - 10.3389/fcell.2022.901026
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan and Clark, Andrew G.",
year = "2022",
abstract = "Collective cell migration on extracellular matrix (ECM) networks is a key biological process involved in development, tissue homeostasis and diseases such as metastatic cancer. During invasion of epithelial cancers, cell clusters migrate through the surrounding stroma, which is comprised primarily of networks of collagen-I fibers. There is growing evidence that the rheological and topological properties of collagen networks can impact cell behavior and cell migration dynamics. During migration, cells exert mechanical forces on their substrate, resulting in an active remodeling of ECM networks that depends not only on the forces produced, but also on the molecular mechanisms that dictate network rheology. One aspect of collagen network rheology whose role is emerging as a crucial parameter in dictating cell behavior is network viscoelasticity. Dynamic reorganization of ECM networks can induce local changes in network organization and mechanics, which can further feed back on cell migration dynamics and cell-cell rearrangement. A number of studies, including many recent publications, have investigated the mechanisms underlying structural changes to collagen networks in response to mechanical force as well as the role of collagen rheology and topology in regulating cell behavior. In this mini-review, we explore the cause-consequence relationship between collagen network viscoelasticity and cell rearrangements at various spatiotemporal scales. We focus on structural alterations of collagen-I networks during collective cell migration and discuss the main rheological parameters, and in particular the role of viscoelasticity, which can contribute to local matrix stiffening during cell movement and can elicit changes in cell dynamics.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Cell and Developmental Biology",
title = "Collective Cell Migration on Collagen-I Networks: The Impact of Matrix Viscoelasticity",
pages = "901026",
volume = "10",
doi = "10.3389/fcell.2022.901026"
}

Pajić-Lijaković, I., Milivojević, M.,& Clark, A. G.. (2022). Collective Cell Migration on Collagen-I Networks: The Impact of Matrix Viscoelasticity. in Frontiers in Cell and Developmental Biology
Frontiers Media S.A.., 10.
https://doi.org/10.3389/fcell.2022.901026

Pajić-Lijaković I, Milivojević M, Clark AG. Collective Cell Migration on Collagen-I Networks: The Impact of Matrix Viscoelasticity. in Frontiers in Cell and Developmental Biology. 2022;10:null-901026.
doi:10.3389/fcell.2022.901026 .

Pajić-Lijaković, Ivana, Milivojević, Milan, Clark, Andrew G., "Collective Cell Migration on Collagen-I Networks: The Impact of Matrix Viscoelasticity" in Frontiers in Cell and Developmental Biology, 10 (2022),
https://doi.org/10.3389/fcell.2022.901026 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5383
AB  - Cell rearrangement caused by collective cell migration (CCM) during free expansion of epithelial monolayers has become a landmark in our current understanding of fundamental biological processes such as tissue development, regeneration, wound healing or cancer invasion. Cell spreading causes formation of mechanical waves which has a feedback effect on cell rearrangement and can lead to the cell jamming state. The mechanical waves describe oscillatory changes in cell velocity, as well as, the rheological parameters that affect them. The velocity oscillations, obtained at a time scale of hours, are in the form of forward and backward flows. Collision of forward and backward flows can induce an increase in the cell compressive stress accompanied with cell packing density which have a feedback impact on cell mobility, tissue viscoelasticity and alters the tissue stiffness. The tissue stiffness depends on the cell packing density and the active/passive (i.e. migrating/resting) state of single cells and can be used as an indicator of cell jamming state transition. Since cell stiffness can be measured it may directly show in which state the multicellular system is. In this work a review of existing modeling approaches is given along with assortment of published experimental findings, in order to invite experimentalists to test given theoretical considerations in multicellular systems.
PB  - Elsevier Ltd.
T2  - Progress in Biophysics and Molecular Biology
T1  - The role of viscoelasticity in long time cell rearrangement
EP  - 71
SP  - 60
VL  - 173
DO  - 10.1016/j.pbiomolbio.2022.05.005
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2022",
abstract = "Cell rearrangement caused by collective cell migration (CCM) during free expansion of epithelial monolayers has become a landmark in our current understanding of fundamental biological processes such as tissue development, regeneration, wound healing or cancer invasion. Cell spreading causes formation of mechanical waves which has a feedback effect on cell rearrangement and can lead to the cell jamming state. The mechanical waves describe oscillatory changes in cell velocity, as well as, the rheological parameters that affect them. The velocity oscillations, obtained at a time scale of hours, are in the form of forward and backward flows. Collision of forward and backward flows can induce an increase in the cell compressive stress accompanied with cell packing density which have a feedback impact on cell mobility, tissue viscoelasticity and alters the tissue stiffness. The tissue stiffness depends on the cell packing density and the active/passive (i.e. migrating/resting) state of single cells and can be used as an indicator of cell jamming state transition. Since cell stiffness can be measured it may directly show in which state the multicellular system is. In this work a review of existing modeling approaches is given along with assortment of published experimental findings, in order to invite experimentalists to test given theoretical considerations in multicellular systems.",
publisher = "Elsevier Ltd.",
journal = "Progress in Biophysics and Molecular Biology",
title = "The role of viscoelasticity in long time cell rearrangement",
pages = "71-60",
volume = "173",
doi = "10.1016/j.pbiomolbio.2022.05.005"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2022). The role of viscoelasticity in long time cell rearrangement. in Progress in Biophysics and Molecular Biology
Elsevier Ltd.., 173, 60-71.
https://doi.org/10.1016/j.pbiomolbio.2022.05.005

Pajić-Lijaković I, Milivojević M. The role of viscoelasticity in long time cell rearrangement. in Progress in Biophysics and Molecular Biology. 2022;173:60-71.
doi:10.1016/j.pbiomolbio.2022.05.005 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "The role of viscoelasticity in long time cell rearrangement" in Progress in Biophysics and Molecular Biology, 173 (2022):60-71,
https://doi.org/10.1016/j.pbiomolbio.2022.05.005 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Guevorkian, Karine
AU  - Barriga, Elias H.
AU  - Munoz, Jose J.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4829
AB  - This issue gathers exciting multi-disciplinary work relating viscoelasticity and collective cell remodeling within various biological processes such as morphogenesis, tumorigenesis, and wound healing. Viscoelasticity is influenced by energy transfer and dissipation during cell rearrangement at various time and space scales. Cumulative structural changes at a subcellular level have effects on viscoelasticity at a supracellular level. Established configurations of migrating cells and the rate of their change, which significantly regulate viscoelasticity at a supracellular level, have the impact on the cohesiveness inhomogeneity and various mechanical and biochemical processes at a subcellular level. This Research Topic aims to connect the macroscopic viscoelastic parameters with the individual and collective cell response. Consideration of biochemical, biophysical and bio-mechanical aspects responsible for tissue remodeling, intercalation, and migration were discussed on various multicellular systems under in vivo and in vitro conditions. Thus in this Research Topic we aim to provide a state-of-the-art view about the current knowledge related to viscoelasticity caused by collective cell remodeling and adhesive contractile properties, covering a plethora of phenomena such as: 1) single cell response under stretched monolayers modeled with an improved Vertex model, 2) adhesion percolation within a tissue as an important factor which influences its viscoelasticity, 3) the active turbulence caused by collective cell migration accompanied with the generation of mechanical waves, 4) cell jamming state transitions, and 5) viscoelastic response characterization in liver diseases. Alternative techniques to measure and control cell rearrangement under various experimental conditions are also considered, including atomic force microscopy measurements and various elastography techniques. This Research Topic provides an overview of the current understanding of various: biological, biochemical, biophysical and mechanical aspects of cell remodeling. The inter-relation between cell remodeling and tissue viscoelasticity was discussed by emphasizing the relevant rheological parameters, the way of their measurement under in vivo/ in vitro conditions, and the strategy of multi-scale constitutive modeling.
T2  - Frontiers in Physics
T1  - Editorial: Viscoelasticity: From Individual Cell Behavior to Collective Tissue Remodeling
VL  - 9
DO  - 10.3389/fphy.2021.773096
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Guevorkian, Karine and Barriga, Elias H. and Munoz, Jose J.",
year = "2021",
abstract = "This issue gathers exciting multi-disciplinary work relating viscoelasticity and collective cell remodeling within various biological processes such as morphogenesis, tumorigenesis, and wound healing. Viscoelasticity is influenced by energy transfer and dissipation during cell rearrangement at various time and space scales. Cumulative structural changes at a subcellular level have effects on viscoelasticity at a supracellular level. Established configurations of migrating cells and the rate of their change, which significantly regulate viscoelasticity at a supracellular level, have the impact on the cohesiveness inhomogeneity and various mechanical and biochemical processes at a subcellular level. This Research Topic aims to connect the macroscopic viscoelastic parameters with the individual and collective cell response. Consideration of biochemical, biophysical and bio-mechanical aspects responsible for tissue remodeling, intercalation, and migration were discussed on various multicellular systems under in vivo and in vitro conditions. Thus in this Research Topic we aim to provide a state-of-the-art view about the current knowledge related to viscoelasticity caused by collective cell remodeling and adhesive contractile properties, covering a plethora of phenomena such as: 1) single cell response under stretched monolayers modeled with an improved Vertex model, 2) adhesion percolation within a tissue as an important factor which influences its viscoelasticity, 3) the active turbulence caused by collective cell migration accompanied with the generation of mechanical waves, 4) cell jamming state transitions, and 5) viscoelastic response characterization in liver diseases. Alternative techniques to measure and control cell rearrangement under various experimental conditions are also considered, including atomic force microscopy measurements and various elastography techniques. This Research Topic provides an overview of the current understanding of various: biological, biochemical, biophysical and mechanical aspects of cell remodeling. The inter-relation between cell remodeling and tissue viscoelasticity was discussed by emphasizing the relevant rheological parameters, the way of their measurement under in vivo/ in vitro conditions, and the strategy of multi-scale constitutive modeling.",
journal = "Frontiers in Physics",
title = "Editorial: Viscoelasticity: From Individual Cell Behavior to Collective Tissue Remodeling",
volume = "9",
doi = "10.3389/fphy.2021.773096"
}

Pajić-Lijaković, I., Guevorkian, K., Barriga, E. H.,& Munoz, J. J.. (2021). Editorial: Viscoelasticity: From Individual Cell Behavior to Collective Tissue Remodeling. in Frontiers in Physics, 9.
https://doi.org/10.3389/fphy.2021.773096

Pajić-Lijaković I, Guevorkian K, Barriga EH, Munoz JJ. Editorial: Viscoelasticity: From Individual Cell Behavior to Collective Tissue Remodeling. in Frontiers in Physics. 2021;9.
doi:10.3389/fphy.2021.773096 .

Pajić-Lijaković, Ivana, Guevorkian, Karine, Barriga, Elias H., Munoz, Jose J., "Editorial: Viscoelasticity: From Individual Cell Behavior to Collective Tissue Remodeling" in Frontiers in Physics, 9 (2021),
https://doi.org/10.3389/fphy.2021.773096 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4868
AB  - Although collective cell migration (CCM) is a highly coordinated migratory mode, perturbations in the form of jamming state transitions and vice versa often occur even in 2D. These perturbations are involved in various biological processes, such as embryogenesis, wound healing, and cancer invasion. CCM induces accumulation of cell residual stress, which has a feedback impact to cell packing density. Density-mediated change of cell mobility influences the state of viscoelasticity of multicellular systems and on that base the jamming state transition. Although a good comprehension of how cells collectively migrate by following molecular rules has been generated, the impact of cellular rearrangements on cell viscoelasticity remains less understood, thus considering that the density-driven evolution of viscoelasticity caused by reduction of cell mobility could result in a powerful tool in order to address the contribution of cell jamming state transition in CCM and help to understand this important but still a controversial topic. In this work a review of existing literature in CCM modeling is given along with an assortment of published experimental findings, in order to invite experimentalists to test the given theoretical considerations in multicellular systems. In addition, five viscoelastic states gained within three regimes: (1) convective regime, (2) conductive regime, and (3) damped-conductive regime, which were discussed with special emphasis of jamming and unjamming states.
T2  - European Physical Journal Plus
T1  - Viscoelasticity and cell jamming state transition
IS  - 7
VL  - 136
DO  - 10.1140/epjp/s13360-021-01730-3
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2021",
abstract = "Although collective cell migration (CCM) is a highly coordinated migratory mode, perturbations in the form of jamming state transitions and vice versa often occur even in 2D. These perturbations are involved in various biological processes, such as embryogenesis, wound healing, and cancer invasion. CCM induces accumulation of cell residual stress, which has a feedback impact to cell packing density. Density-mediated change of cell mobility influences the state of viscoelasticity of multicellular systems and on that base the jamming state transition. Although a good comprehension of how cells collectively migrate by following molecular rules has been generated, the impact of cellular rearrangements on cell viscoelasticity remains less understood, thus considering that the density-driven evolution of viscoelasticity caused by reduction of cell mobility could result in a powerful tool in order to address the contribution of cell jamming state transition in CCM and help to understand this important but still a controversial topic. In this work a review of existing literature in CCM modeling is given along with an assortment of published experimental findings, in order to invite experimentalists to test the given theoretical considerations in multicellular systems. In addition, five viscoelastic states gained within three regimes: (1) convective regime, (2) conductive regime, and (3) damped-conductive regime, which were discussed with special emphasis of jamming and unjamming states.",
journal = "European Physical Journal Plus",
title = "Viscoelasticity and cell jamming state transition",
number = "7",
volume = "136",
doi = "10.1140/epjp/s13360-021-01730-3"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2021). Viscoelasticity and cell jamming state transition. in European Physical Journal Plus, 136(7).
https://doi.org/10.1140/epjp/s13360-021-01730-3

Pajić-Lijaković I, Milivojević M. Viscoelasticity and cell jamming state transition. in European Physical Journal Plus. 2021;136(7).
doi:10.1140/epjp/s13360-021-01730-3 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Viscoelasticity and cell jamming state transition" in European Physical Journal Plus, 136, no. 7 (2021),
https://doi.org/10.1140/epjp/s13360-021-01730-3 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4965
AB  - Collective cell migration (CCM), a highly coordinated and fine-tuned migratory mode, is involved in a plethora of biological processes, such as embryogenesis, tissue repair and cancer invasion. Although a good comprehension of how cells collectively migrate by following molecular rules has been generated, the impact of cellular rearrangements on collective migration remains less understood. Thus, considering CCM from a multi-scale quantitative approach could result in a powerful tool to address the contribution of cellular rearrangements in CCM and help to understand this important but still controversial topic. In this work, a review of existing literature in CCM modeling at different scales is given along with assortment of published experimental findings, to invite experimentalists to test given theoretical considerations in multicellular systems. In addition, three different time and space scales (free or weakly connected cells, cluster of cells and collision fronts of different cells clusters) are considered and the multi-scale nature of those processes was discussed with special emphasis of jamming and unjamming states.
T2  - European Biophysics Journal With Biophysics Letters
T1  - Multiscale nature of cell rearrangement caused by collective cell migration
EP  - 14
IS  - 1
SP  - 1
VL  - 50
DO  - 10.1007/s00249-021-01496-7
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2021",
abstract = "Collective cell migration (CCM), a highly coordinated and fine-tuned migratory mode, is involved in a plethora of biological processes, such as embryogenesis, tissue repair and cancer invasion. Although a good comprehension of how cells collectively migrate by following molecular rules has been generated, the impact of cellular rearrangements on collective migration remains less understood. Thus, considering CCM from a multi-scale quantitative approach could result in a powerful tool to address the contribution of cellular rearrangements in CCM and help to understand this important but still controversial topic. In this work, a review of existing literature in CCM modeling at different scales is given along with assortment of published experimental findings, to invite experimentalists to test given theoretical considerations in multicellular systems. In addition, three different time and space scales (free or weakly connected cells, cluster of cells and collision fronts of different cells clusters) are considered and the multi-scale nature of those processes was discussed with special emphasis of jamming and unjamming states.",
journal = "European Biophysics Journal With Biophysics Letters",
title = "Multiscale nature of cell rearrangement caused by collective cell migration",
pages = "14-1",
number = "1",
volume = "50",
doi = "10.1007/s00249-021-01496-7"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2021). Multiscale nature of cell rearrangement caused by collective cell migration. in European Biophysics Journal With Biophysics Letters, 50(1), 1-14.
https://doi.org/10.1007/s00249-021-01496-7

Pajić-Lijaković I, Milivojević M. Multiscale nature of cell rearrangement caused by collective cell migration. in European Biophysics Journal With Biophysics Letters. 2021;50(1):1-14.
doi:10.1007/s00249-021-01496-7 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Multiscale nature of cell rearrangement caused by collective cell migration" in European Biophysics Journal With Biophysics Letters, 50, no. 1 (2021):1-14,
https://doi.org/10.1007/s00249-021-01496-7 . .

TY  - JOUR
AU  - Obradović, Nataša
AU  - Pajić-Lijaković, Ivana
AU  - Krunić, Tanja
AU  - Belović, Miona
AU  - Rakin, Marica
AU  - Bugarski, Branko
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4463
AB  - The suitability of natural hydrogel carriers with probiotic starter culture as whey beverages supplements was examined by assessing their rheological and structural changes during the fermentation and gastrointestinal conditions. Effect of encapsulated cells on the carrier structure is of great importance for the selection of proper material for the preparation of functional supplements. The structural changes of the chitosan-coated alginate/whey carriers were considered based on (1) cell viability and the carrier average volume vs. time (2) the storage and loss modulus vs. time obtained under low oscillator strain conditions, (3) FTIR analysis and (4) SEM cross-sectional observation of the hydrogel carriers. The presence of chitosan coating and fermentation conditions increased cell viability up to 9.01 +/- 0.18 (log CFU/g). According to our results, the encapsulated cells induce weakening of carriers under the gastric conditions but improve their mechanical stability under the intestinal condition. The mechanical behaviour of carriers was also considered in order to formulate the rheological constitutive model equation for describing the irreversible structural changes under the gastric and intestinal conditions. The cell leakage under the gastric condition after the 2 h was less than 5%. Carriers are rapidly degraded under the intestinal condition which ensures the release of cells and provides their beneficial effects on the host health. Our results indicate that this type of coated carrier is suitable to be used for encapsulation of probiotic starter culture in the production of fermented whey-based products.
PB  - Springer, New York
T2  - Food Biophysics
T1  - Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions
EP  - 31
IS  - 1
SP  - 18
VL  - 15
DO  - 10.1007/s11483-019-09598-8
ER  - 

@article{
author = "Obradović, Nataša and Pajić-Lijaković, Ivana and Krunić, Tanja and Belović, Miona and Rakin, Marica and Bugarski, Branko",
year = "2020",
abstract = "The suitability of natural hydrogel carriers with probiotic starter culture as whey beverages supplements was examined by assessing their rheological and structural changes during the fermentation and gastrointestinal conditions. Effect of encapsulated cells on the carrier structure is of great importance for the selection of proper material for the preparation of functional supplements. The structural changes of the chitosan-coated alginate/whey carriers were considered based on (1) cell viability and the carrier average volume vs. time (2) the storage and loss modulus vs. time obtained under low oscillator strain conditions, (3) FTIR analysis and (4) SEM cross-sectional observation of the hydrogel carriers. The presence of chitosan coating and fermentation conditions increased cell viability up to 9.01 +/- 0.18 (log CFU/g). According to our results, the encapsulated cells induce weakening of carriers under the gastric conditions but improve their mechanical stability under the intestinal condition. The mechanical behaviour of carriers was also considered in order to formulate the rheological constitutive model equation for describing the irreversible structural changes under the gastric and intestinal conditions. The cell leakage under the gastric condition after the 2 h was less than 5%. Carriers are rapidly degraded under the intestinal condition which ensures the release of cells and provides their beneficial effects on the host health. Our results indicate that this type of coated carrier is suitable to be used for encapsulation of probiotic starter culture in the production of fermented whey-based products.",
publisher = "Springer, New York",
journal = "Food Biophysics",
title = "Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions",
pages = "31-18",
number = "1",
volume = "15",
doi = "10.1007/s11483-019-09598-8"
}

Obradović, N., Pajić-Lijaković, I., Krunić, T., Belović, M., Rakin, M.,& Bugarski, B.. (2020). Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions. in Food Biophysics
Springer, New York., 15(1), 18-31.
https://doi.org/10.1007/s11483-019-09598-8

Obradović N, Pajić-Lijaković I, Krunić T, Belović M, Rakin M, Bugarski B. Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions. in Food Biophysics. 2020;15(1):18-31.
doi:10.1007/s11483-019-09598-8 .

Obradović, Nataša, Pajić-Lijaković, Ivana, Krunić, Tanja, Belović, Miona, Rakin, Marica, Bugarski, Branko, "Effect of Encapsulated Probiotic Starter Culture on Rheological and Structural Properties of Natural Hydrogel Carriers Affected by Fermentation and Gastrointestinal Conditions" in Food Biophysics, 15, no. 1 (2020):18-31,
https://doi.org/10.1007/s11483-019-09598-8 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4448
AB  - Various types of mechanical waves, such as propagative waves and standing waves, are observed during 2D collective cell migration. Propagative waves are generated during monolayer free expansion, whereas standing waves are generated during swirling motion of a confluent monolayer. Significant attempts have been made to describe the main characteristics of mechanical waves obtained within various experimental systems. However, much less attention is paid to correlate the viscoelasticity with the generated oscillatory instabilities. Mechanical waves have recognized during flow of various viscoelastic systems under low Reynolds number and called "the elastic turbulence." In addition to Reynolds number, Weissenberg number is needed for characterizing the elastic turbulence. The viscoelastic resistive force generated during collective cell migration caused by a residual stress accumulation is capable of inducing apparent inertial effects by balancing with other forces such as the surface tension force, the traction force, and the resultant force responsible for cell migration. The resultant force represents a product of various biochemical processes such as cell signaling and gene expression. The force balance induces (1) forward flow and backward flow in the direction of cell migration as characteristics of the propagative waves and (2) inflow and outflow perpendicular to the direction of migration as characteristics of the standing waves. The apparent inertial effects are essential for appearing the elastic turbulence and represent the characteristic of (1) the backward flow during the monolayer free expansion and (2) the inflow during the cell swirling motion within a confluent monolayer.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Physics
T1  - Mechanical Oscillations in 2D Collective Cell Migration: The Elastic Turbulence
VL  - 8
DO  - 10.3389/fphy.2020.585681
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2020",
abstract = "Various types of mechanical waves, such as propagative waves and standing waves, are observed during 2D collective cell migration. Propagative waves are generated during monolayer free expansion, whereas standing waves are generated during swirling motion of a confluent monolayer. Significant attempts have been made to describe the main characteristics of mechanical waves obtained within various experimental systems. However, much less attention is paid to correlate the viscoelasticity with the generated oscillatory instabilities. Mechanical waves have recognized during flow of various viscoelastic systems under low Reynolds number and called "the elastic turbulence." In addition to Reynolds number, Weissenberg number is needed for characterizing the elastic turbulence. The viscoelastic resistive force generated during collective cell migration caused by a residual stress accumulation is capable of inducing apparent inertial effects by balancing with other forces such as the surface tension force, the traction force, and the resultant force responsible for cell migration. The resultant force represents a product of various biochemical processes such as cell signaling and gene expression. The force balance induces (1) forward flow and backward flow in the direction of cell migration as characteristics of the propagative waves and (2) inflow and outflow perpendicular to the direction of migration as characteristics of the standing waves. The apparent inertial effects are essential for appearing the elastic turbulence and represent the characteristic of (1) the backward flow during the monolayer free expansion and (2) the inflow during the cell swirling motion within a confluent monolayer.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Physics",
title = "Mechanical Oscillations in 2D Collective Cell Migration: The Elastic Turbulence",
volume = "8",
doi = "10.3389/fphy.2020.585681"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2020). Mechanical Oscillations in 2D Collective Cell Migration: The Elastic Turbulence. in Frontiers in Physics
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fphy.2020.585681

Pajić-Lijaković I, Milivojević M. Mechanical Oscillations in 2D Collective Cell Migration: The Elastic Turbulence. in Frontiers in Physics. 2020;8.
doi:10.3389/fphy.2020.585681 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Mechanical Oscillations in 2D Collective Cell Migration: The Elastic Turbulence" in Frontiers in Physics, 8 (2020),
https://doi.org/10.3389/fphy.2020.585681 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4500
AB  - Viscoelasticity of multicellular systems caused by collective cell migration depends on (1) viscoelasticity of migrating clusters, (2) viscoelasticity of surrounding resting cells and (3) the size, slip effects and thickness of the biointerface. A previously developed model for a sharp biointerface is expanded for the case of a finite biointerface based on thermodynamic and rheological considerations to estimate the influence of the biointerface properties on viscoelasticity. These properties of the interface layer are one of the key factors which influence the overall properties of the mixture, such as its viscoelasticity. Sliding of cell clusters through dense surroundings induces generation of significant shear stress, within the biointerface, which influences (1) the active (contractile) or passive state of single cells and (2) the state of cell-cell adhesion contacts. Cells retain collectivity in migration patterns even upon a reduction of cell-cell adhesion caused by stress generation. A greater size to the biointerface leads to the weakening of multicellular systems for the same volume fraction of migrating cells due to energy dissipation. Various factors such as (1) increase of the interface size, (2) reduction in slip effects under the constant thickness of the biointerface and (3) decrease in the biointerface thickness under constant slip effects induce an increase of the shear rate as well as the overall energy dissipation and can lead to circular cell movement within the biointerface layer. Additional experiments at subcellular and cellular levels are needed to determine the influence of mechanical factors on collective cell migration.
PB  - Springer, New York
T2  - European Biophysics Journal with Biophysics Letters
T1  - Viscoelasticity of multicellular systems caused by collective cell migration: dynamics at the biointerface
EP  - 265
IS  - 3-4
SP  - 253
VL  - 49
DO  - 10.1007/s00249-020-01431-2
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2020",
abstract = "Viscoelasticity of multicellular systems caused by collective cell migration depends on (1) viscoelasticity of migrating clusters, (2) viscoelasticity of surrounding resting cells and (3) the size, slip effects and thickness of the biointerface. A previously developed model for a sharp biointerface is expanded for the case of a finite biointerface based on thermodynamic and rheological considerations to estimate the influence of the biointerface properties on viscoelasticity. These properties of the interface layer are one of the key factors which influence the overall properties of the mixture, such as its viscoelasticity. Sliding of cell clusters through dense surroundings induces generation of significant shear stress, within the biointerface, which influences (1) the active (contractile) or passive state of single cells and (2) the state of cell-cell adhesion contacts. Cells retain collectivity in migration patterns even upon a reduction of cell-cell adhesion caused by stress generation. A greater size to the biointerface leads to the weakening of multicellular systems for the same volume fraction of migrating cells due to energy dissipation. Various factors such as (1) increase of the interface size, (2) reduction in slip effects under the constant thickness of the biointerface and (3) decrease in the biointerface thickness under constant slip effects induce an increase of the shear rate as well as the overall energy dissipation and can lead to circular cell movement within the biointerface layer. Additional experiments at subcellular and cellular levels are needed to determine the influence of mechanical factors on collective cell migration.",
publisher = "Springer, New York",
journal = "European Biophysics Journal with Biophysics Letters",
title = "Viscoelasticity of multicellular systems caused by collective cell migration: dynamics at the biointerface",
pages = "265-253",
number = "3-4",
volume = "49",
doi = "10.1007/s00249-020-01431-2"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2020). Viscoelasticity of multicellular systems caused by collective cell migration: dynamics at the biointerface. in European Biophysics Journal with Biophysics Letters
Springer, New York., 49(3-4), 253-265.
https://doi.org/10.1007/s00249-020-01431-2

Pajić-Lijaković I, Milivojević M. Viscoelasticity of multicellular systems caused by collective cell migration: dynamics at the biointerface. in European Biophysics Journal with Biophysics Letters. 2020;49(3-4):253-265.
doi:10.1007/s00249-020-01431-2 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Viscoelasticity of multicellular systems caused by collective cell migration: dynamics at the biointerface" in European Biophysics Journal with Biophysics Letters, 49, no. 3-4 (2020):253-265,
https://doi.org/10.1007/s00249-020-01431-2 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4511
AB  - Stress generation during collective cell migration represents one of the key factors which influence the configuration of migrating cells, viscoelasticity of multicellular systems and their inter-relation. Local generation of stress (normal and shear) is significant even in 2D. Normal stress is primarily accumulated within a core region of migrating cell clusters during their movement through the dense environment and during the collisions of migrating cell clusters caused by uncorrelated motility. Shear stress can be significant within perturbed boundary layers around migrating clusters. Cells are more sensitive to the action of shear stress compared with normal stress. Shear stress of a few Pa significantly influences cell state. Prior studies have shown that collectively migrating cells move in such a way to minimize this stress, but it has not yet been determined how cells effectively minimize it. Deeper insight into possible cell mechanisms for minimizing undesirable shear stress would be of great importance because it may help to direct morphogenesis, accelerate wound healing or prevent cancer invasion. In the proposed model three primary mechanisms in which cells may reduce shear are given: decreasing speed, tissue thickening, and/or reducing slip effects. Suggestions obtained from the proposed model indicate a need for further experimental studies that will reveal whether the heterogeneity in the cell-cell adhesion types correlates well with the stiffness inhomogeneity, or changes in the adhesion clustering, cytoskeletal linkage or some other modification to the adhesion complex (adherens junctions or tight junctions) are occurring to influence overall adhesive strength.
PB  - Elsevier Sci Ltd, Oxford
T2  - Journal of Biomechanics
T1  - Collective cell migration and residual stress accumulation: Rheological consideration
VL  - 108
DO  - 10.1016/j.jbiomech.2020.109898
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2020",
abstract = "Stress generation during collective cell migration represents one of the key factors which influence the configuration of migrating cells, viscoelasticity of multicellular systems and their inter-relation. Local generation of stress (normal and shear) is significant even in 2D. Normal stress is primarily accumulated within a core region of migrating cell clusters during their movement through the dense environment and during the collisions of migrating cell clusters caused by uncorrelated motility. Shear stress can be significant within perturbed boundary layers around migrating clusters. Cells are more sensitive to the action of shear stress compared with normal stress. Shear stress of a few Pa significantly influences cell state. Prior studies have shown that collectively migrating cells move in such a way to minimize this stress, but it has not yet been determined how cells effectively minimize it. Deeper insight into possible cell mechanisms for minimizing undesirable shear stress would be of great importance because it may help to direct morphogenesis, accelerate wound healing or prevent cancer invasion. In the proposed model three primary mechanisms in which cells may reduce shear are given: decreasing speed, tissue thickening, and/or reducing slip effects. Suggestions obtained from the proposed model indicate a need for further experimental studies that will reveal whether the heterogeneity in the cell-cell adhesion types correlates well with the stiffness inhomogeneity, or changes in the adhesion clustering, cytoskeletal linkage or some other modification to the adhesion complex (adherens junctions or tight junctions) are occurring to influence overall adhesive strength.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Biomechanics",
title = "Collective cell migration and residual stress accumulation: Rheological consideration",
volume = "108",
doi = "10.1016/j.jbiomech.2020.109898"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2020). Collective cell migration and residual stress accumulation: Rheological consideration. in Journal of Biomechanics
Elsevier Sci Ltd, Oxford., 108.
https://doi.org/10.1016/j.jbiomech.2020.109898

Pajić-Lijaković I, Milivojević M. Collective cell migration and residual stress accumulation: Rheological consideration. in Journal of Biomechanics. 2020;108.
doi:10.1016/j.jbiomech.2020.109898 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Collective cell migration and residual stress accumulation: Rheological consideration" in Journal of Biomechanics, 108 (2020),
https://doi.org/10.1016/j.jbiomech.2020.109898 . .

TY  - JOUR
AU  - Dajić-Stevanović, Zora
AU  - Sieniawska, Elwira
AU  - Glowniak, Kazimierz
AU  - Obradović, Nataša
AU  - Pajić-Lijaković, Ivana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4517
AB  - Essential oils (EOs) and their main constituents, the terpenes, are widely studied, mostly relating to their antioxidant ability and bioactivity, such as antimicrobial, anticancer, anti-inflammatory, and range of other actions in the living systems. However, there is limited information on their bioavailability, especially upon clinical studies. Having in mind both strong biological effects and health benefits of EOs and their specific physicochemical properties (volatility, lipophilic character, low water solubility or insolubility, viscosity, expressed odor, concentration-dependent toxicity, etc.), there is a need for their encapsulation for target delivery. Encapsulation of EOs and their constituents is the prerequisite for enhancing their oxidative stability, thermostability, photostability, shelf life, and biological activity. We considered various carrier types such a (1) monophase and polyphase polysaccharide hydrogel carriers, (2) polysaccharide-protein carriers, and (3) lipid carriers in the context of physicochemical and engineering factors. Physicochemical factors are encapsulation efficiency, chemical stability under gastric conditions, mechanical stability, and thermal stability of carrier matrices. Choice of carrier material also determines the encapsulation technique. Consequently, the engineering factors are related to the advantage and disadvantage of various encapsulation techniques frequently used in the literature. In addition, it was intended to address the interactions between (1) main carrier components, such as polysaccharides, proteins, and lipids themselves (in order to form chemically and mechanically stable structure); (2) main carrier components with pepsin under gastric conditions (in order to form resistant material under gastric conditions); and (3) main carrier components with EOs (in order to enhance encapsulation efficiency), as a necessary precondition for whole process optimization. Finally, different sources for obtaining natural carrier macromolecules are surveyed, especially the agro-waste materials and agricultural and food by-products. This review article highlights the bioavailability aspects of encapsulated EOs and physicochemical and engineering factors concerning natural macromolecule carriers for their target delivery and application.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application
VL  - 8
DO  - 10.3389/fbioe.2020.00563
ER  - 

@article{
author = "Dajić-Stevanović, Zora and Sieniawska, Elwira and Glowniak, Kazimierz and Obradović, Nataša and Pajić-Lijaković, Ivana",
year = "2020",
abstract = "Essential oils (EOs) and their main constituents, the terpenes, are widely studied, mostly relating to their antioxidant ability and bioactivity, such as antimicrobial, anticancer, anti-inflammatory, and range of other actions in the living systems. However, there is limited information on their bioavailability, especially upon clinical studies. Having in mind both strong biological effects and health benefits of EOs and their specific physicochemical properties (volatility, lipophilic character, low water solubility or insolubility, viscosity, expressed odor, concentration-dependent toxicity, etc.), there is a need for their encapsulation for target delivery. Encapsulation of EOs and their constituents is the prerequisite for enhancing their oxidative stability, thermostability, photostability, shelf life, and biological activity. We considered various carrier types such a (1) monophase and polyphase polysaccharide hydrogel carriers, (2) polysaccharide-protein carriers, and (3) lipid carriers in the context of physicochemical and engineering factors. Physicochemical factors are encapsulation efficiency, chemical stability under gastric conditions, mechanical stability, and thermal stability of carrier matrices. Choice of carrier material also determines the encapsulation technique. Consequently, the engineering factors are related to the advantage and disadvantage of various encapsulation techniques frequently used in the literature. In addition, it was intended to address the interactions between (1) main carrier components, such as polysaccharides, proteins, and lipids themselves (in order to form chemically and mechanically stable structure); (2) main carrier components with pepsin under gastric conditions (in order to form resistant material under gastric conditions); and (3) main carrier components with EOs (in order to enhance encapsulation efficiency), as a necessary precondition for whole process optimization. Finally, different sources for obtaining natural carrier macromolecules are surveyed, especially the agro-waste materials and agricultural and food by-products. This review article highlights the bioavailability aspects of encapsulated EOs and physicochemical and engineering factors concerning natural macromolecule carriers for their target delivery and application.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application",
volume = "8",
doi = "10.3389/fbioe.2020.00563"
}

Dajić-Stevanović, Z., Sieniawska, E., Glowniak, K., Obradović, N.,& Pajić-Lijaković, I.. (2020). Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application. in Frontiers in Bioengineering and Biotechnology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fbioe.2020.00563

Dajić-Stevanović Z, Sieniawska E, Glowniak K, Obradović N, Pajić-Lijaković I. Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application. in Frontiers in Bioengineering and Biotechnology. 2020;8.
doi:10.3389/fbioe.2020.00563 .

Dajić-Stevanović, Zora, Sieniawska, Elwira, Glowniak, Kazimierz, Obradović, Nataša, Pajić-Lijaković, Ivana, "Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application" in Frontiers in Bioengineering and Biotechnology, 8 (2020),
https://doi.org/10.3389/fbioe.2020.00563 . .