TY  - JOUR
AU  - Veljković, Milica
AU  - Banjanac, Katarina
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Simović, Milica
AU  - Bezbradica, Dejan
PY  - 2024-08
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7425
AB  - Maple syrup, a popular natural sweetener has a high content of sucrose, whose consumption is linked to different health issues such as obesity and diabetes. Hence, within this paper, the conversion of sucrose to prebiotics (fructo-oligosaccharides, FOS) was proposed as a promising approach to obtaining a healthier, value-added product. Enzymatic conversion was optimized with respect to key experimental factors, and thereafter derived immobilized preparation of fructosyltransferase (FTase) from Pectinex® Ultra SP-L (FTase-epoxy Purolite, 255 IU/g support) was successfully utilized to produce novel functional product in ten consecutive reaction cycles. The product, obtained under optimal conditions (60 ◦C, 7.65 IU/mL, 12 h), resulted in 56.0% FOS, 16.7% sucrose, and 27.3% monosaccharides of total carbohydrates, leading to a 1.6-fold reduction in caloric content. The obtained products` prebiotic potential toward the probiotic strain Lactobacillus plantarum 299v was demonstrated. The changes in physico-chemical and sensorial characteristics were esteemed as negligible.
PB  - Elsevier Ltd.
T2  - Food Chemistry
T1  - Production of prebiotic enriched maple syrup through enzymatic conversion of sucrose into fructo-oligosaccharides
SP  - 139180
VL  - 449
DO  - 10.1016/j.foodchem.2024.139180
ER  - 

@article{
author = "Veljković, Milica and Banjanac, Katarina and Milivojević, Ana and Ćorović, Marija and Simović, Milica and Bezbradica, Dejan",
year = "2024-08",
abstract = "Maple syrup, a popular natural sweetener has a high content of sucrose, whose consumption is linked to different health issues such as obesity and diabetes. Hence, within this paper, the conversion of sucrose to prebiotics (fructo-oligosaccharides, FOS) was proposed as a promising approach to obtaining a healthier, value-added product. Enzymatic conversion was optimized with respect to key experimental factors, and thereafter derived immobilized preparation of fructosyltransferase (FTase) from Pectinex® Ultra SP-L (FTase-epoxy Purolite, 255 IU/g support) was successfully utilized to produce novel functional product in ten consecutive reaction cycles. The product, obtained under optimal conditions (60 ◦C, 7.65 IU/mL, 12 h), resulted in 56.0% FOS, 16.7% sucrose, and 27.3% monosaccharides of total carbohydrates, leading to a 1.6-fold reduction in caloric content. The obtained products` prebiotic potential toward the probiotic strain Lactobacillus plantarum 299v was demonstrated. The changes in physico-chemical and sensorial characteristics were esteemed as negligible.",
publisher = "Elsevier Ltd.",
journal = "Food Chemistry",
title = "Production of prebiotic enriched maple syrup through enzymatic conversion of sucrose into fructo-oligosaccharides",
pages = "139180",
volume = "449",
doi = "10.1016/j.foodchem.2024.139180"
}

Veljković, M., Banjanac, K., Milivojević, A., Ćorović, M., Simović, M.,& Bezbradica, D.. (2024-08). Production of prebiotic enriched maple syrup through enzymatic conversion of sucrose into fructo-oligosaccharides. in Food Chemistry
Elsevier Ltd.., 449, 139180.
https://doi.org/10.1016/j.foodchem.2024.139180

Veljković M, Banjanac K, Milivojević A, Ćorović M, Simović M, Bezbradica D. Production of prebiotic enriched maple syrup through enzymatic conversion of sucrose into fructo-oligosaccharides. in Food Chemistry. 2024;449:139180.
doi:10.1016/j.foodchem.2024.139180 .

Veljković, Milica, Banjanac, Katarina, Milivojević, Ana, Ćorović, Marija, Simović, Milica, Bezbradica, Dejan, "Production of prebiotic enriched maple syrup through enzymatic conversion of sucrose into fructo-oligosaccharides" in Food Chemistry, 449 (2024-08):139180,
https://doi.org/10.1016/j.foodchem.2024.139180 . .

TY  - JOUR
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Veljković, Milica
AU  - Bezbradica, Dejan
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7384
AB  - Fruit pomaces are agro-industrial by-products obtained in large quantities by the juice production industry, hence their reutilization is a suitable way to minimize environmental impact by transforming them into different value-added products. Considering the wide abundance and bioactive potential of berries pomaces, raspberry, strawberry, blackcurrant, and chokeberry pomace were chosen to examine and compare polyphenol content, antioxidant activity, and skin prebiotic capacity of their extracts. Extracts of 5.28 to 13.39 mg GAE ⋅ g−1 DM total polyphenol content were obtained, with significant differences in polyphenol abundance between classes. Relative antioxidant capacity index and global antioxidant score revealed that the blackcurrant pomace extract exhibited the highest antioxidant capacity. Staphylococcus epidermidis growth was promoted by lower extract concentrations, while higher concentrations showed inhibitory effects. On the other hand, Staphylococcus aureus was inhibited more strongly and no stimulation was detected in the tested concentration range (.025–.1 mg GAE ⋅ mL−1). In co-cultured experiments, a S. aureus/S. epidermidis rebalancing effect was proven for all tested extracts, whereas the best prebiotic capacity of 2.84 was achieved at.05 mg GAE⋅ mL−1 of raspberry pomace extract. In conclusion, berry pomace extracts could be valorized by extraction of their phytochemicals which are potent antioxidants and emerging prebiotics for topical application.
PB  - Taylor and Francis Ltd.
T2  - International Journal of Fruit Science
T1  - Berries Pomace Valorization: From Waste to Potent Antioxidants and Emerging Skin Prebiotics
EP  - 101
IS  - 1
SP  - 85
VL  - 24
DO  - 10.1080/15538362.2024.2322743
ER  - 

@article{
author = "Petrov Ivanković, Anja and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Banjanac, Katarina and Veljković, Milica and Bezbradica, Dejan",
year = "2024",
abstract = "Fruit pomaces are agro-industrial by-products obtained in large quantities by the juice production industry, hence their reutilization is a suitable way to minimize environmental impact by transforming them into different value-added products. Considering the wide abundance and bioactive potential of berries pomaces, raspberry, strawberry, blackcurrant, and chokeberry pomace were chosen to examine and compare polyphenol content, antioxidant activity, and skin prebiotic capacity of their extracts. Extracts of 5.28 to 13.39 mg GAE ⋅ g−1 DM total polyphenol content were obtained, with significant differences in polyphenol abundance between classes. Relative antioxidant capacity index and global antioxidant score revealed that the blackcurrant pomace extract exhibited the highest antioxidant capacity. Staphylococcus epidermidis growth was promoted by lower extract concentrations, while higher concentrations showed inhibitory effects. On the other hand, Staphylococcus aureus was inhibited more strongly and no stimulation was detected in the tested concentration range (.025–.1 mg GAE ⋅ mL−1). In co-cultured experiments, a S. aureus/S. epidermidis rebalancing effect was proven for all tested extracts, whereas the best prebiotic capacity of 2.84 was achieved at.05 mg GAE⋅ mL−1 of raspberry pomace extract. In conclusion, berry pomace extracts could be valorized by extraction of their phytochemicals which are potent antioxidants and emerging prebiotics for topical application.",
publisher = "Taylor and Francis Ltd.",
journal = "International Journal of Fruit Science",
title = "Berries Pomace Valorization: From Waste to Potent Antioxidants and Emerging Skin Prebiotics",
pages = "101-85",
number = "1",
volume = "24",
doi = "10.1080/15538362.2024.2322743"
}

Petrov Ivanković, A., Ćorović, M., Milivojević, A., Simović, M., Banjanac, K., Veljković, M.,& Bezbradica, D.. (2024). Berries Pomace Valorization: From Waste to Potent Antioxidants and Emerging Skin Prebiotics. in International Journal of Fruit Science
Taylor and Francis Ltd.., 24(1), 85-101.
https://doi.org/10.1080/15538362.2024.2322743

Petrov Ivanković A, Ćorović M, Milivojević A, Simović M, Banjanac K, Veljković M, Bezbradica D. Berries Pomace Valorization: From Waste to Potent Antioxidants and Emerging Skin Prebiotics. in International Journal of Fruit Science. 2024;24(1):85-101.
doi:10.1080/15538362.2024.2322743 .

Petrov Ivanković, Anja, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Banjanac, Katarina, Veljković, Milica, Bezbradica, Dejan, "Berries Pomace Valorization: From Waste to Potent Antioxidants and Emerging Skin Prebiotics" in International Journal of Fruit Science, 24, no. 1 (2024):85-101,
https://doi.org/10.1080/15538362.2024.2322743 . .

TY  - JOUR
AU  - Šekuljica, Nataša
AU  - Jakovetić Tanasković, Sonja
AU  - Mijalković, Jelena
AU  - Simović, Milica
AU  - Pavlović, Neda
AU  - Đorđević, Nikola
AU  - Culetu, Alina
AU  - Gazikalović, Ivana
AU  - Luković, Nevena
AU  - Bakrač, Jelena
AU  - Knežević-Jugović, Zorica
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7084
AB  - Research background. The development of a novel process for the production of xy-looligosaccharides (XOS) based on the 4R concept is made possible by the integration of numerous techniques, especially enzymatic modification together with the physical pre-treatment of renewable materials. This study aims to integrate the use of agricultural wastes for the production of xylanase by a new strain of Penicillium sp. and value-added products, XOS. Experimental approach. For the production of xylanase, a solid-state fermentation was performed using wheat bran as substrate. To obtain the most active crude extract of xy-lanase, the time frame of cultivation was first adjusted. Then, the downstream process for xylanase purification was developed by combining different membrane separation units with size exclusion chromatography. Further characterisation included determination of the optimal pH and temperature, determination of the molecular mass of the purified xy-lanase and analysis of kinetic parameters. Subsequently, the hydrolytic ability of the par-tially purified xylanase in the hydrolysis of alkali-extracted hemicellulose from soybean hulls was investigated. Results and conclusions. Our results show that Penicillium rubens produced extracellular xylanase at a yield of 21 U/g during solid-state fermentation. Using two ultrafiltration membranes of 10 and 3 kDa in combination with size exclusion chromatography, a yield of 49 % and 13-fold purification of xylanase was achieved. The purified xylanase (35 kDa) cleaved linear bonds β-(1→4) in beechwood xylan at a maximum rate of 0.64 μmol/ (min·mg) and a Michaelis constant of 44 mg/mL. At pH=6 and 45 °C, the purified xylanase showed its maximum activity. The xylanase produced showed a high ability to hydrolyse the hemicellulose fraction isolated from soybean hulls, as confirmed by thin-layer chro-matography. In the hydrothermally pretreated hemicellulose hydrolysate, the content of XOS with different degrees of polymerisation was detected, while in the non-pretreated hemicellulose hydrolysate, the content of xylotriose and glucose was confirmed. Novelty and scientific contribution. Future research focusing on the creation of new en-zymatic pathways for use in processes to convert renewable materials into value-added products can draw on our findings.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls
EP  - 450
IS  - 4
SP  - 439
VL  - 61
DO  - 10.17113/ftb.61.04.23.8073
ER  - 

@article{
author = "Šekuljica, Nataša and Jakovetić Tanasković, Sonja and Mijalković, Jelena and Simović, Milica and Pavlović, Neda and Đorđević, Nikola and Culetu, Alina and Gazikalović, Ivana and Luković, Nevena and Bakrač, Jelena and Knežević-Jugović, Zorica",
year = "2023",
abstract = "Research background. The development of a novel process for the production of xy-looligosaccharides (XOS) based on the 4R concept is made possible by the integration of numerous techniques, especially enzymatic modification together with the physical pre-treatment of renewable materials. This study aims to integrate the use of agricultural wastes for the production of xylanase by a new strain of Penicillium sp. and value-added products, XOS. Experimental approach. For the production of xylanase, a solid-state fermentation was performed using wheat bran as substrate. To obtain the most active crude extract of xy-lanase, the time frame of cultivation was first adjusted. Then, the downstream process for xylanase purification was developed by combining different membrane separation units with size exclusion chromatography. Further characterisation included determination of the optimal pH and temperature, determination of the molecular mass of the purified xy-lanase and analysis of kinetic parameters. Subsequently, the hydrolytic ability of the par-tially purified xylanase in the hydrolysis of alkali-extracted hemicellulose from soybean hulls was investigated. Results and conclusions. Our results show that Penicillium rubens produced extracellular xylanase at a yield of 21 U/g during solid-state fermentation. Using two ultrafiltration membranes of 10 and 3 kDa in combination with size exclusion chromatography, a yield of 49 % and 13-fold purification of xylanase was achieved. The purified xylanase (35 kDa) cleaved linear bonds β-(1→4) in beechwood xylan at a maximum rate of 0.64 μmol/ (min·mg) and a Michaelis constant of 44 mg/mL. At pH=6 and 45 °C, the purified xylanase showed its maximum activity. The xylanase produced showed a high ability to hydrolyse the hemicellulose fraction isolated from soybean hulls, as confirmed by thin-layer chro-matography. In the hydrothermally pretreated hemicellulose hydrolysate, the content of XOS with different degrees of polymerisation was detected, while in the non-pretreated hemicellulose hydrolysate, the content of xylotriose and glucose was confirmed. Novelty and scientific contribution. Future research focusing on the creation of new en-zymatic pathways for use in processes to convert renewable materials into value-added products can draw on our findings.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls",
pages = "450-439",
number = "4",
volume = "61",
doi = "10.17113/ftb.61.04.23.8073"
}

Šekuljica, N., Jakovetić Tanasković, S., Mijalković, J., Simović, M., Pavlović, N., Đorđević, N., Culetu, A., Gazikalović, I., Luković, N., Bakrač, J.,& Knežević-Jugović, Z.. (2023). Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls. in Food Technology and Biotechnology
University of Zagreb., 61(4), 439-450.
https://doi.org/10.17113/ftb.61.04.23.8073

Šekuljica N, Jakovetić Tanasković S, Mijalković J, Simović M, Pavlović N, Đorđević N, Culetu A, Gazikalović I, Luković N, Bakrač J, Knežević-Jugović Z. Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls. in Food Technology and Biotechnology. 2023;61(4):439-450.
doi:10.17113/ftb.61.04.23.8073 .

Šekuljica, Nataša, Jakovetić Tanasković, Sonja, Mijalković, Jelena, Simović, Milica, Pavlović, Neda, Đorđević, Nikola, Culetu, Alina, Gazikalović, Ivana, Luković, Nevena, Bakrač, Jelena, Knežević-Jugović, Zorica, "Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls" in Food Technology and Biotechnology, 61, no. 4 (2023):439-450,
https://doi.org/10.17113/ftb.61.04.23.8073 . .

TY  - JOUR
AU  - Veljković, Milica
AU  - Stepanović, Relja
AU  - Banjanac, Katarina
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Milivojević, Milan
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5247
AB  - Within this study, the investigation of potential selective immobilization of fructosyltransferase (FTase) derived from Aspergillus aculeatus (from commercial enzyme preparation Pectinex® Ultra SP-L) onto the polystyrenic macroporous resin Purolite® A109 and its further utilization for the synthesis of a highly valuable group of prebiotic compounds named fructo-oligosaccharides (FOS), was performed. The obtained results suggest that partial purification of fructosyltransferase from complex enzyme mixture with predominant pectinase activity (ratio of activity immobilization yields around 20.0) occurred by performing a simple immobilization protocol, provided that immobilization lasted for 5 h using an enzyme concentration of 50 mg g−1 of support under slightly acidic conditions (pH 6.0). Immobilized preparation was thereafter successfully utilized for FOS synthesis (56–59 % FOS of total sugars) in both a batch and air-lift reactor system. Operational stability study proved the exceptionally high potential of immobilized preparation for reproducible and cost-effective application, since 90 % of initial activity was preserved after ten consecutive reaction cycles. Thereafter, a prospectus for continuous production of FOS in the air-lift reactor was assayed. Achieved yields, 53 % FOS of total sugars for at least 9 h, indicated the great suitability of the obtained immobilized preparation for application in large-scale systems.
PB  - Korean Society of Industrial Engineering Chemistry
T2  - Journal of Industrial and Engineering Chemistry
T1  - Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109
EP  - 156
SP  - 149
VL  - 117
DO  - 10.1016/j.jiec.2022.09.051
ER  - 

@article{
author = "Veljković, Milica and Stepanović, Relja and Banjanac, Katarina and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Milivojević, Milan and Bezbradica, Dejan",
year = "2023",
abstract = "Within this study, the investigation of potential selective immobilization of fructosyltransferase (FTase) derived from Aspergillus aculeatus (from commercial enzyme preparation Pectinex® Ultra SP-L) onto the polystyrenic macroporous resin Purolite® A109 and its further utilization for the synthesis of a highly valuable group of prebiotic compounds named fructo-oligosaccharides (FOS), was performed. The obtained results suggest that partial purification of fructosyltransferase from complex enzyme mixture with predominant pectinase activity (ratio of activity immobilization yields around 20.0) occurred by performing a simple immobilization protocol, provided that immobilization lasted for 5 h using an enzyme concentration of 50 mg g−1 of support under slightly acidic conditions (pH 6.0). Immobilized preparation was thereafter successfully utilized for FOS synthesis (56–59 % FOS of total sugars) in both a batch and air-lift reactor system. Operational stability study proved the exceptionally high potential of immobilized preparation for reproducible and cost-effective application, since 90 % of initial activity was preserved after ten consecutive reaction cycles. Thereafter, a prospectus for continuous production of FOS in the air-lift reactor was assayed. Achieved yields, 53 % FOS of total sugars for at least 9 h, indicated the great suitability of the obtained immobilized preparation for application in large-scale systems.",
publisher = "Korean Society of Industrial Engineering Chemistry",
journal = "Journal of Industrial and Engineering Chemistry",
title = "Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109",
pages = "156-149",
volume = "117",
doi = "10.1016/j.jiec.2022.09.051"
}

Veljković, M., Stepanović, R., Banjanac, K., Ćorović, M., Milivojević, A., Simović, M., Milivojević, M.,& Bezbradica, D.. (2023). Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109. in Journal of Industrial and Engineering Chemistry
Korean Society of Industrial Engineering Chemistry., 117, 149-156.
https://doi.org/10.1016/j.jiec.2022.09.051

Veljković M, Stepanović R, Banjanac K, Ćorović M, Milivojević A, Simović M, Milivojević M, Bezbradica D. Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109. in Journal of Industrial and Engineering Chemistry. 2023;117:149-156.
doi:10.1016/j.jiec.2022.09.051 .

Veljković, Milica, Stepanović, Relja, Banjanac, Katarina, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Milivojević, Milan, Bezbradica, Dejan, "Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109" in Journal of Industrial and Engineering Chemistry, 117 (2023):149-156,
https://doi.org/10.1016/j.jiec.2022.09.051 . .

TY  - JOUR
AU  - Petrov Ivanković, Anja
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Jansen, Patrick
AU  - Vukoičić, Ana
AU  - van den Bogaard, Ellen
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6870
AB  - Background Blackcurrant is a rich source of polyphenols with proven physiological activity, that have lately been classified as emerging prebiotics, making its extracts suitable for application in functional food and bioactive cosmetics. However, these biomolecules are trapped in cell wall structures and nowadays, enzymatic hydrolysis is recognised as powerful tool for extraction efficiency improvement in an environmentally sound manner. This study aimed to optimize enzyme-assisted extraction to maximize yields of blackcurrant polyphenols. In addition, their prebiotic potential was tested by examining their influence on typical skin microbiota representatives as model microorganisms—beneficial coagulase-negative Staphylococcus epidermidis and two opportunistic pathogens, Staphylococcus aureus and Cutibacterium acnes. Results Among four examined commercial enzyme preparations, Viscozyme® L and Rohapect® MC, demonstrated the highest potential as extraction enhancers by increasing antioxidant activity, total polyphenol, and flavonoid contents. Furthermore, their synergetic effect enabled 95.3% increase in polyphenol concentration compared to conventional extraction with a maximum of 27.6 mg gallic acid equivalents (GAE)/g dry matter (DM) at a total enzyme concentration of 0.05 mL/g DM (Viscozyme® L to Rohapect® MC ratio 2:1) after 60 min. HPLC profiles of two samples showed differences in polyphenol composition indicating simultaneous extraction–biotransformation. At a concentration of 0.025 mg GAE/mL, PAS (prebiotic activity score) values were 0.096 and 0.172 for conventionally and enzymatically obtained extract, respectively. Moderate inhibitory effect of optimal extract concentration on Cutibacterium acnes was shown, as well. Hydrogel prepared with optimal extract concentration showed improved hydration effect, decreased irritability and increased dermatological compatibility comparing to basic formulation. Conclusions Overall results demonstrated that blackcurrant extract obtained under optimized conditions using enzymes possesses an emerging prebiotic potential for selective skin microbiota stimulation.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Chemical and Biological Technologies in Agriculture
T1  - In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives
IS  - 1
SP  - 125
VL  - 10
DO  - 10.1186/s40538-023-00502-8
ER  - 

@article{
author = "Petrov Ivanković, Anja and Milivojević, Ana and Ćorović, Marija and Simović, Milica and Banjanac, Katarina and Jansen, Patrick and Vukoičić, Ana and van den Bogaard, Ellen and Bezbradica, Dejan",
year = "2023",
abstract = "Background Blackcurrant is a rich source of polyphenols with proven physiological activity, that have lately been classified as emerging prebiotics, making its extracts suitable for application in functional food and bioactive cosmetics. However, these biomolecules are trapped in cell wall structures and nowadays, enzymatic hydrolysis is recognised as powerful tool for extraction efficiency improvement in an environmentally sound manner. This study aimed to optimize enzyme-assisted extraction to maximize yields of blackcurrant polyphenols. In addition, their prebiotic potential was tested by examining their influence on typical skin microbiota representatives as model microorganisms—beneficial coagulase-negative Staphylococcus epidermidis and two opportunistic pathogens, Staphylococcus aureus and Cutibacterium acnes. Results Among four examined commercial enzyme preparations, Viscozyme® L and Rohapect® MC, demonstrated the highest potential as extraction enhancers by increasing antioxidant activity, total polyphenol, and flavonoid contents. Furthermore, their synergetic effect enabled 95.3% increase in polyphenol concentration compared to conventional extraction with a maximum of 27.6 mg gallic acid equivalents (GAE)/g dry matter (DM) at a total enzyme concentration of 0.05 mL/g DM (Viscozyme® L to Rohapect® MC ratio 2:1) after 60 min. HPLC profiles of two samples showed differences in polyphenol composition indicating simultaneous extraction–biotransformation. At a concentration of 0.025 mg GAE/mL, PAS (prebiotic activity score) values were 0.096 and 0.172 for conventionally and enzymatically obtained extract, respectively. Moderate inhibitory effect of optimal extract concentration on Cutibacterium acnes was shown, as well. Hydrogel prepared with optimal extract concentration showed improved hydration effect, decreased irritability and increased dermatological compatibility comparing to basic formulation. Conclusions Overall results demonstrated that blackcurrant extract obtained under optimized conditions using enzymes possesses an emerging prebiotic potential for selective skin microbiota stimulation.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Chemical and Biological Technologies in Agriculture",
title = "In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives",
number = "1",
pages = "125",
volume = "10",
doi = "10.1186/s40538-023-00502-8"
}

Petrov Ivanković, A., Milivojević, A., Ćorović, M., Simović, M., Banjanac, K., Jansen, P., Vukoičić, A., van den Bogaard, E.,& Bezbradica, D.. (2023). In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives. in Chemical and Biological Technologies in Agriculture
Springer Science and Business Media Deutschland GmbH., 10(1), 125.
https://doi.org/10.1186/s40538-023-00502-8

Petrov Ivanković A, Milivojević A, Ćorović M, Simović M, Banjanac K, Jansen P, Vukoičić A, van den Bogaard E, Bezbradica D. In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives. in Chemical and Biological Technologies in Agriculture. 2023;10(1):125.
doi:10.1186/s40538-023-00502-8 .

Petrov Ivanković, Anja, Milivojević, Ana, Ćorović, Marija, Simović, Milica, Banjanac, Katarina, Jansen, Patrick, Vukoičić, Ana, van den Bogaard, Ellen, Bezbradica, Dejan, "In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives" in Chemical and Biological Technologies in Agriculture, 10, no. 1 (2023):125,
https://doi.org/10.1186/s40538-023-00502-8 . .

TY  - CONF
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6962
AB  - Fructo-oligosaccharides (FOS) are linear bioactive molecules consisting of one
terminal glucose and other fructose units and belong to the group of prebiotics with
scientifically proven benefits for human health. Physiological active compounds like
trisaccharides (FOS3), tetrasaccharides (FOS4) and pentasaccharides (FOS5) can be
produced from sucrose using enzyme-fructosyltransferase. The reaction mixture obtained in
this way contains, in addition to the desired prebiotics, glucose, fructose and an unreacted
amount of sucrose. Direct incorporation in products of food and pharmaceutical origin is
limited due to the presence of unwanted carbohydrates (mono- and disaccharide), and to
increase the functional value of the mixture, the attention was focused on their removal. In
this regard, membrane separation techniques present a very suitable solution for obtaining a
product with a higher FOS proportion. This study aimed to determine the optimal conditions
for purification process of produced FOS mixture using nanofiltration membrane modules.
Therefore, the influence of membranes with different MWCO (300-500Da and 600-800Da),
temperature (20-45°C) and carbohydrate concentration in feed solution (1-5%) at a constant
flow rate of 22 mL/min was investigated. From the initial mixture containing 60.2% FOS,
29.7% monosaccharides and 10.1% sucrose, after purification process under determined
optimal conditions (membrane 300-500Da, temperature 35°C and carbohydrate concentration
3%), the product with 88.8% FOS, 10.0% sucrose and 1.2% monosaccharides in total
carbohydrates was obtained. Purification yield was greater than 95% and the rejection
coefficients for glucose, fructose, sucrose, FOS3, FOS4 and FOS5 were 0.17, 0.20, 0.95,
0.99, 1.00 and 1.00, respectively. Based on the calculated purification factor, it can be
concluded that the initial mixture is refined 1.47 times. This result suggests a significant
increase in the purity of the final mixture, which can be used as a sugar substitute or addition
to numerous products that would be suitable for all consumers, including those suffering from
diabetes.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES
SP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6962
ER  - 

@conference{
author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Petrov Ivanković, Anja and Ćorović, Marija and Bezbradica, Dejan",
year = "2023",
abstract = "Fructo-oligosaccharides (FOS) are linear bioactive molecules consisting of one
terminal glucose and other fructose units and belong to the group of prebiotics with
scientifically proven benefits for human health. Physiological active compounds like
trisaccharides (FOS3), tetrasaccharides (FOS4) and pentasaccharides (FOS5) can be
produced from sucrose using enzyme-fructosyltransferase. The reaction mixture obtained in
this way contains, in addition to the desired prebiotics, glucose, fructose and an unreacted
amount of sucrose. Direct incorporation in products of food and pharmaceutical origin is
limited due to the presence of unwanted carbohydrates (mono- and disaccharide), and to
increase the functional value of the mixture, the attention was focused on their removal. In
this regard, membrane separation techniques present a very suitable solution for obtaining a
product with a higher FOS proportion. This study aimed to determine the optimal conditions
for purification process of produced FOS mixture using nanofiltration membrane modules.
Therefore, the influence of membranes with different MWCO (300-500Da and 600-800Da),
temperature (20-45°C) and carbohydrate concentration in feed solution (1-5%) at a constant
flow rate of 22 mL/min was investigated. From the initial mixture containing 60.2% FOS,
29.7% monosaccharides and 10.1% sucrose, after purification process under determined
optimal conditions (membrane 300-500Da, temperature 35°C and carbohydrate concentration
3%), the product with 88.8% FOS, 10.0% sucrose and 1.2% monosaccharides in total
carbohydrates was obtained. Purification yield was greater than 95% and the rejection
coefficients for glucose, fructose, sucrose, FOS3, FOS4 and FOS5 were 0.17, 0.20, 0.95,
0.99, 1.00 and 1.00, respectively. Based on the calculated purification factor, it can be
concluded that the initial mixture is refined 1.47 times. This result suggests a significant
increase in the purity of the final mixture, which can be used as a sugar substitute or addition
to numerous products that would be suitable for all consumers, including those suffering from
diabetes.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES",
pages = "86",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6962"
}

Veljković, M., Simović, M., Banjanac, K., Petrov Ivanković, A., Ćorović, M.,& Bezbradica, D.. (2023). NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 86.
https://hdl.handle.net/21.15107/rcub_technorep_6962

Veljković M, Simović M, Banjanac K, Petrov Ivanković A, Ćorović M, Bezbradica D. NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:86.
https://hdl.handle.net/21.15107/rcub_technorep_6962 .

Veljković, Milica, Simović, Milica, Banjanac, Katarina, Petrov Ivanković, Anja, Ćorović, Marija, Bezbradica, Dejan, "NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):86,
https://hdl.handle.net/21.15107/rcub_technorep_6962 .

TY  - CONF
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Vukoičić, Ana
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6843
AB  - With the increasing interest in functional products, prebiotics that selectively stimulate growth and activity of good bacteria in human gut, gained attention from both general and scientific community. The range of food products containing prebiotic oligosaccharides is rapidly expanding, causing their intensified production. Currently, there are a few recognized prebiotics, amongst which, fructo-oligosaccharides (FOS) proved to be particularly interesting owing to the confirmed positive effect on human wellbeing (restoring microbiota balance, improving immunity and lipid metabolism, enhancing vitamin production and calcium absorption) and exceptional physicochemical qualities. Namely, good taste quality, low sweetness, and low caloric value make them highly applicable supplement in food industry. Fructo-oligosaccharides are scarcely present in natural sources and nowadays are mainly produced by enzymatic conversion of sucrose and sucrose-rich substrates. Bearing in mind the complexity of synthesis process, resulting reaction mixture besides FOS (mixture of tri-, tetra- and pentasaccharides) contains significant portion of sucrose and monosaccharides. Within this study, efficient method for obtaining high-purity product upon sucrose conversion using fructosyltransferase (Pectinex® Ultra SP-L) was developed through detailed optimization of membrane separation processes. Under determined optimal conditions for nanofiltration (temperature of 35 °C, concentration of initial sugars of 3% and flow rate of 25 mL/min), undesired components (monosaccharides and partially sucrose) were removed from the initial mixture (58% FOS in total carbohydrates) and 1.48-fold increase the proportion of targeted bioactive components was achieved. Thus, product of high purity (86% FOS in total carbohydrates) was obtained with remarkable recovery yields of 90%. The prebiotic activity of the obtained product was confirmed in in vitro tests with several microbial cultures comprising representatives of beneficial (genera Lactobacillus and Saccharomyces) and pathogenic (Escherichia coli) gut microbiota. Having said that, it can be concluded that high-purity prebiotic product with great potential for application in different value-added food industry products was obtained.
PB  - Belgrade : University, Faculty of Geography
C3  - Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023
T1  - Effective approach for obtaining high-purity prebiotics using membrane separation technology
SP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6843
ER  - 

@conference{
author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Milivojević, Ana and Ćorović, Marija and Vukoičić, Ana and Bezbradica, Dejan",
year = "2023",
abstract = "With the increasing interest in functional products, prebiotics that selectively stimulate growth and activity of good bacteria in human gut, gained attention from both general and scientific community. The range of food products containing prebiotic oligosaccharides is rapidly expanding, causing their intensified production. Currently, there are a few recognized prebiotics, amongst which, fructo-oligosaccharides (FOS) proved to be particularly interesting owing to the confirmed positive effect on human wellbeing (restoring microbiota balance, improving immunity and lipid metabolism, enhancing vitamin production and calcium absorption) and exceptional physicochemical qualities. Namely, good taste quality, low sweetness, and low caloric value make them highly applicable supplement in food industry. Fructo-oligosaccharides are scarcely present in natural sources and nowadays are mainly produced by enzymatic conversion of sucrose and sucrose-rich substrates. Bearing in mind the complexity of synthesis process, resulting reaction mixture besides FOS (mixture of tri-, tetra- and pentasaccharides) contains significant portion of sucrose and monosaccharides. Within this study, efficient method for obtaining high-purity product upon sucrose conversion using fructosyltransferase (Pectinex® Ultra SP-L) was developed through detailed optimization of membrane separation processes. Under determined optimal conditions for nanofiltration (temperature of 35 °C, concentration of initial sugars of 3% and flow rate of 25 mL/min), undesired components (monosaccharides and partially sucrose) were removed from the initial mixture (58% FOS in total carbohydrates) and 1.48-fold increase the proportion of targeted bioactive components was achieved. Thus, product of high purity (86% FOS in total carbohydrates) was obtained with remarkable recovery yields of 90%. The prebiotic activity of the obtained product was confirmed in in vitro tests with several microbial cultures comprising representatives of beneficial (genera Lactobacillus and Saccharomyces) and pathogenic (Escherichia coli) gut microbiota. Having said that, it can be concluded that high-purity prebiotic product with great potential for application in different value-added food industry products was obtained.",
publisher = "Belgrade : University, Faculty of Geography",
journal = "Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023",
title = "Effective approach for obtaining high-purity prebiotics using membrane separation technology",
pages = "25",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6843"
}

Veljković, M., Simović, M., Banjanac, K., Milivojević, A., Ćorović, M., Vukoičić, A.,& Bezbradica, D.. (2023). Effective approach for obtaining high-purity prebiotics using membrane separation technology. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023
Belgrade : University, Faculty of Geography., 25.
https://hdl.handle.net/21.15107/rcub_technorep_6843

Veljković M, Simović M, Banjanac K, Milivojević A, Ćorović M, Vukoičić A, Bezbradica D. Effective approach for obtaining high-purity prebiotics using membrane separation technology. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023. 2023;:25.
https://hdl.handle.net/21.15107/rcub_technorep_6843 .

Veljković, Milica, Simović, Milica, Banjanac, Katarina, Milivojević, Ana, Ćorović, Marija, Vukoičić, Ana, Bezbradica, Dejan, "Effective approach for obtaining high-purity prebiotics using membrane separation technology" in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023 (2023):25,
https://hdl.handle.net/21.15107/rcub_technorep_6843 .

TY  - CONF
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Veljković, Milica
AU  - Vukoičić, Ana
AU  - Simović, Milica
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6946
AB  - Berries are known to be one of the richest sources of polyphenols which can offer
various health benefits. Dietary supplementation with berries has a positive influence on the
gut microbiota, which directly affects overall health, including skin health. However, topical
application of berry polyphenols has been used mainly for its antioxidant activity to prevent
premature aging and improve the skin's appearance. Therefore, this study examined the
content of different polyphenol classes of cranberry (Vaccinium macrocarpa), chokeberry
(Aronia melanocarpa) and blueberry (Vaccinium angustifolium) extracts, investigating their
antioxidant properties and potential impact on skin as topical prebiotics. The prebiotic
capacity of these extracts in applied concentrations range of 0.015-0.05 mg GAE/mL, was
determined against two cutaneous bacteria - beneficial Staphylococcus epidermidis and
opportunistic pathogen Staphylococcus aureus, since the disrupted balance between them
may contribute worsening of atopic dermatitis. The obtained results showed that the total
polyphenol content was highest in chokeberry extract (9.88 mg GAE/g DM), followed by
cranberry extract (8.78 mg GAE/g DM), and the lowest in blueberry extract (6.48 mg GAE/g
DM). Chokeberry extract was also richest in flavonoids, flavonols, anthocyanins and phenolic
acids. Notably, cranberry extract had the highest concentration of tannins, almost three times
higher compared to blueberry extract. According to DPPH and ABTS methods, the
antioxidant activity was significantly high in chokeberry extract, while FRAP method
revealed that cranberry extract is the most potent antioxidant. Regarding prebiotic capacity,
positive values (0.10-0.48) were observed at all concentrations of cranberry extract, with a
trend indicating a decrease in prebiotic capacity as polyphenol concentration increases.
Prebiotic capacities of blueberry and chokeberry extracts had either negative values or values
equal to zero, indicating that these extracts do not positively impact the microorganisms’
ratio. Obtained findings suggest cranberry extract potential for enhancing both antioxidant
defense of skin and rebalancing cutaneous microbiota.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS
SP  - 29
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6946
ER  - 

@conference{
author = "Petrov Ivanković, Anja and Ćorović, Marija and Milivojević, Ana and Veljković, Milica and Vukoičić, Ana and Simović, Milica and Bezbradica, Dejan",
year = "2023",
abstract = "Berries are known to be one of the richest sources of polyphenols which can offer
various health benefits. Dietary supplementation with berries has a positive influence on the
gut microbiota, which directly affects overall health, including skin health. However, topical
application of berry polyphenols has been used mainly for its antioxidant activity to prevent
premature aging and improve the skin's appearance. Therefore, this study examined the
content of different polyphenol classes of cranberry (Vaccinium macrocarpa), chokeberry
(Aronia melanocarpa) and blueberry (Vaccinium angustifolium) extracts, investigating their
antioxidant properties and potential impact on skin as topical prebiotics. The prebiotic
capacity of these extracts in applied concentrations range of 0.015-0.05 mg GAE/mL, was
determined against two cutaneous bacteria - beneficial Staphylococcus epidermidis and
opportunistic pathogen Staphylococcus aureus, since the disrupted balance between them
may contribute worsening of atopic dermatitis. The obtained results showed that the total
polyphenol content was highest in chokeberry extract (9.88 mg GAE/g DM), followed by
cranberry extract (8.78 mg GAE/g DM), and the lowest in blueberry extract (6.48 mg GAE/g
DM). Chokeberry extract was also richest in flavonoids, flavonols, anthocyanins and phenolic
acids. Notably, cranberry extract had the highest concentration of tannins, almost three times
higher compared to blueberry extract. According to DPPH and ABTS methods, the
antioxidant activity was significantly high in chokeberry extract, while FRAP method
revealed that cranberry extract is the most potent antioxidant. Regarding prebiotic capacity,
positive values (0.10-0.48) were observed at all concentrations of cranberry extract, with a
trend indicating a decrease in prebiotic capacity as polyphenol concentration increases.
Prebiotic capacities of blueberry and chokeberry extracts had either negative values or values
equal to zero, indicating that these extracts do not positively impact the microorganisms’
ratio. Obtained findings suggest cranberry extract potential for enhancing both antioxidant
defense of skin and rebalancing cutaneous microbiota.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS",
pages = "29",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6946"
}

Petrov Ivanković, A., Ćorović, M., Milivojević, A., Veljković, M., Vukoičić, A., Simović, M.,& Bezbradica, D.. (2023). DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 29.
https://hdl.handle.net/21.15107/rcub_technorep_6946

Petrov Ivanković A, Ćorović M, Milivojević A, Veljković M, Vukoičić A, Simović M, Bezbradica D. DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:29.
https://hdl.handle.net/21.15107/rcub_technorep_6946 .

Petrov Ivanković, Anja, Ćorović, Marija, Milivojević, Ana, Veljković, Milica, Vukoičić, Ana, Simović, Milica, Bezbradica, Dejan, "DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):29,
https://hdl.handle.net/21.15107/rcub_technorep_6946 .

TY  - CONF
AU  - dos Santos, Leandro Alves
AU  - Banjanac, Katarina
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Mateo, Cesar
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6948
AB  - A food-grade bacterial xylanase enzyme preparation, ROHALASE® SEP-VISCO,
was immobilized by adsorption onto polyethyleneimine (PEI) functionalized magnetic
nanoparticles (MNPs). This process resulted in nanobiocatalysts with optimal characteristics.
Subsequently, this nanobiocatalyst was employed to produce xylo-oligosaccharides (XOS). In
the past decade, magnetic nanoparticles (MNPs) have gained significant interest, finding
diverse applications in biomedicine, biosensor production, food processing, catalysis,
agriculture, and environmental processes. MNPs have proven highly effective for enzyme
immobilization because of their unique and distinguished properties such as high specific
surface area, biocompatibility, and magnetic characteristics. Magnetic characteristics
facilitate the easy removal of MNPs from reaction mixtures, allowing for their efficient reuse.
The functionalization of MNPs through with the cationic polymer PEI can further augment
their ability for enzyme attachment. Generally, xylanases hydrolyses β-1,4-glycosidic
linkages present in xylan which is a major component of the hemicellulosic fraction in the
plant cell walls. Furthermore, xylanases can produce XOS composed of xylose units linked
by β-1,4-xylosidic bonds. XOS have stimulatory effects on the selective growth of human
intestinal microbiota and are frequently defined as prebiotics.
In the present work, the xylanase immobilization efficiency on MNPs-PEI is between
100 and 43 % within the wide range of xylanase concentrations (400-2400 mg/g of support).
Free and immobilized xylanase showed maximal catalytic activity at pH 6.0 and 60°C in
reaction with commercial birchwood xylan (concentration of 1 % w/v). The maximum
activity of 1675 IU/g of support was achieved when immobilization was performed at initial
enzyme concentration of 1250 mg/g of support during 2 h. Since this immobilized
preparation exhibited the activity immobilization yield of 80 % and specific activity of 2.1
mg of proteins/g of support, it has been applied in reaction of XOS synthesis. The MNPsPEI-xylanase was found to produce high yield of XOS from birchwood xylan, indicating its
potential for utilization in feed and food formulations.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6948
ER  - 

@conference{
author = "dos Santos, Leandro Alves and Banjanac, Katarina and Veljković, Milica and Simović, Milica and Mateo, Cesar and Bezbradica, Dejan",
year = "2023",
abstract = "A food-grade bacterial xylanase enzyme preparation, ROHALASE® SEP-VISCO,
was immobilized by adsorption onto polyethyleneimine (PEI) functionalized magnetic
nanoparticles (MNPs). This process resulted in nanobiocatalysts with optimal characteristics.
Subsequently, this nanobiocatalyst was employed to produce xylo-oligosaccharides (XOS). In
the past decade, magnetic nanoparticles (MNPs) have gained significant interest, finding
diverse applications in biomedicine, biosensor production, food processing, catalysis,
agriculture, and environmental processes. MNPs have proven highly effective for enzyme
immobilization because of their unique and distinguished properties such as high specific
surface area, biocompatibility, and magnetic characteristics. Magnetic characteristics
facilitate the easy removal of MNPs from reaction mixtures, allowing for their efficient reuse.
The functionalization of MNPs through with the cationic polymer PEI can further augment
their ability for enzyme attachment. Generally, xylanases hydrolyses β-1,4-glycosidic
linkages present in xylan which is a major component of the hemicellulosic fraction in the
plant cell walls. Furthermore, xylanases can produce XOS composed of xylose units linked
by β-1,4-xylosidic bonds. XOS have stimulatory effects on the selective growth of human
intestinal microbiota and are frequently defined as prebiotics.
In the present work, the xylanase immobilization efficiency on MNPs-PEI is between
100 and 43 % within the wide range of xylanase concentrations (400-2400 mg/g of support).
Free and immobilized xylanase showed maximal catalytic activity at pH 6.0 and 60°C in
reaction with commercial birchwood xylan (concentration of 1 % w/v). The maximum
activity of 1675 IU/g of support was achieved when immobilization was performed at initial
enzyme concentration of 1250 mg/g of support during 2 h. Since this immobilized
preparation exhibited the activity immobilization yield of 80 % and specific activity of 2.1
mg of proteins/g of support, it has been applied in reaction of XOS synthesis. The MNPsPEI-xylanase was found to produce high yield of XOS from birchwood xylan, indicating its
potential for utilization in feed and food formulations.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS",
pages = "34",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6948"
}

dos Santos, L. A., Banjanac, K., Veljković, M., Simović, M., Mateo, C.,& Bezbradica, D.. (2023). IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 34.
https://hdl.handle.net/21.15107/rcub_technorep_6948

dos Santos LA, Banjanac K, Veljković M, Simović M, Mateo C, Bezbradica D. IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:34.
https://hdl.handle.net/21.15107/rcub_technorep_6948 .

dos Santos, Leandro Alves, Banjanac, Katarina, Veljković, Milica, Simović, Milica, Mateo, Cesar, Bezbradica, Dejan, "IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):34,
https://hdl.handle.net/21.15107/rcub_technorep_6948 .

TY  - CONF
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Jaćimovski, Darko
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6871
AB  - Scientific breakthroughs in research of human microbiota revealed that significant number of
diseases related to modern food intake and sedentary lifestyles are caused by dysbiosis of human
microbiota composition, hence substantial increase in interest for products helping restore this balance
(such as probiotics and prebiotics) within food, cosmetic and health industry was observed. In recent
times, prebiotics have been recognized as attractive products due to their higher stability to processing
and storage conditions, lower susceptibility to interaction with other compounds in more complex
formulation and higher reproducibility of their physiological effects [1]. Consequential rapid growth of
market demand brought challenges for biochemical industry, such as requirements to increase
production capacity and decrease costs by development of more efficient enzymatic processes and
product separation.
Majority of prebiotic compounds are classified as oligosaccharides, which can be obtained
either by enzymatic synthesis from compounds of lower degree of polymerization (DP) - for example:
galacto-oligosaccharides (GOS) from lactose and fructo-oligosaccharides (FOS) from sucrose - or by
enzymatic hydrolysis, for example pectic oligosaccharides (POS) from pectin or xylo-oligosaccharides
(XOS) from xylan [2]. In both cases, membrane separation is efficient tool for product purification,
because OS obtained by synthesis can be separated from lower DP compounds using nanofiltrations,
while those obtained by hydrolysis can be separated from compounds of higher DP using ultrafiltration.
Hence, it can be expected that selection of adequate membrane material, pore dimensions and
downstream processing conditions will have important role in development of novel prebiotic
technologies.
In this study nanofiltration membrane module was applied with a purpose of purification of
FOS obtained in enzymatic synthesis using sucrose as substrate. Two strategies were applied:
• purification of reaction mixture obtained after enzymatic synthesis,
• simultaneous enzymatic synthesis and purification in nanofiltration membrane module.
PB  - TECNALIA
C3  - Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain
T1  - Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module
EP  - 85
EP  - 
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6871
ER  - 

@conference{
author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Milivojević, Ana and Ćorović, Marija and Jaćimovski, Darko and Bezbradica, Dejan",
year = "2023",
abstract = "Scientific breakthroughs in research of human microbiota revealed that significant number of
diseases related to modern food intake and sedentary lifestyles are caused by dysbiosis of human
microbiota composition, hence substantial increase in interest for products helping restore this balance
(such as probiotics and prebiotics) within food, cosmetic and health industry was observed. In recent
times, prebiotics have been recognized as attractive products due to their higher stability to processing
and storage conditions, lower susceptibility to interaction with other compounds in more complex
formulation and higher reproducibility of their physiological effects [1]. Consequential rapid growth of
market demand brought challenges for biochemical industry, such as requirements to increase
production capacity and decrease costs by development of more efficient enzymatic processes and
product separation.
Majority of prebiotic compounds are classified as oligosaccharides, which can be obtained
either by enzymatic synthesis from compounds of lower degree of polymerization (DP) - for example:
galacto-oligosaccharides (GOS) from lactose and fructo-oligosaccharides (FOS) from sucrose - or by
enzymatic hydrolysis, for example pectic oligosaccharides (POS) from pectin or xylo-oligosaccharides
(XOS) from xylan [2]. In both cases, membrane separation is efficient tool for product purification,
because OS obtained by synthesis can be separated from lower DP compounds using nanofiltrations,
while those obtained by hydrolysis can be separated from compounds of higher DP using ultrafiltration.
Hence, it can be expected that selection of adequate membrane material, pore dimensions and
downstream processing conditions will have important role in development of novel prebiotic
technologies.
In this study nanofiltration membrane module was applied with a purpose of purification of
FOS obtained in enzymatic synthesis using sucrose as substrate. Two strategies were applied:
• purification of reaction mixture obtained after enzymatic synthesis,
• simultaneous enzymatic synthesis and purification in nanofiltration membrane module.",
publisher = "TECNALIA",
journal = "Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain",
title = "Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module",
pages = "85--84",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6871"
}

Veljković, M., Simović, M., Banjanac, K., Milivojević, A., Ćorović, M., Jaćimovski, D.,& Bezbradica, D.. (2023). Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module. in Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain
TECNALIA., 84-85.
https://hdl.handle.net/21.15107/rcub_technorep_6871

Veljković M, Simović M, Banjanac K, Milivojević A, Ćorović M, Jaćimovski D, Bezbradica D. Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module. in Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain. 2023;:84-85.
https://hdl.handle.net/21.15107/rcub_technorep_6871 .

Veljković, Milica, Simović, Milica, Banjanac, Katarina, Milivojević, Ana, Ćorović, Marija, Jaćimovski, Darko, Bezbradica, Dejan, "Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module" in Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain (2023):84-85,
https://hdl.handle.net/21.15107/rcub_technorep_6871 .

TY  - JOUR
AU  - Pravilović, Radoslava
AU  - Janković, Tamara
AU  - Veljković, Milica
AU  - Todić, Branislav
AU  - Simović, Milica
AU  - Bezbradica, Dejan
AU  - Nikačević, Nikola
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6385
AB  - A new micro-kinetic model of the enzyme-catalyzed synthesis of fructo-oligosaccharides (FOS) was developed. A commercial enzyme mixture Pectinex® Ultra SP-L derived from Aspergillus aculeatus was used. A variety of initial enzyme concentrations (1–5 vol%) and sucrose concentrations (400–600 g/L) were experimentally investigated and included in kinetic modeling. Several variations of kinetic mechanisms and corresponding models have been examined. A hybrid genetic algorithm was used to predict the kinetic parameters simultaneously for all experimental data. The best fitting model has been adopted, and with an average error of 13.34%, it describes the experimental data very well. The influence of initial concentrations on the conversion of sucrose and production of FOS is being carefully investigated. It was shown that the initial sucrose concentration significantly affects the highest level of FOS concentration, but the enzyme concentration controls the time at which maximum is reached as well as the rate of FOS decomposition.
PB  - John Wiley and Sons Inc.
T2  - AIChE Journal
T1  - Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products
IS  - 9
SP  - e18122
VL  - 69
DO  - 10.1002/aic.18122
ER  - 

@article{
author = "Pravilović, Radoslava and Janković, Tamara and Veljković, Milica and Todić, Branislav and Simović, Milica and Bezbradica, Dejan and Nikačević, Nikola",
year = "2023",
abstract = "A new micro-kinetic model of the enzyme-catalyzed synthesis of fructo-oligosaccharides (FOS) was developed. A commercial enzyme mixture Pectinex® Ultra SP-L derived from Aspergillus aculeatus was used. A variety of initial enzyme concentrations (1–5 vol%) and sucrose concentrations (400–600 g/L) were experimentally investigated and included in kinetic modeling. Several variations of kinetic mechanisms and corresponding models have been examined. A hybrid genetic algorithm was used to predict the kinetic parameters simultaneously for all experimental data. The best fitting model has been adopted, and with an average error of 13.34%, it describes the experimental data very well. The influence of initial concentrations on the conversion of sucrose and production of FOS is being carefully investigated. It was shown that the initial sucrose concentration significantly affects the highest level of FOS concentration, but the enzyme concentration controls the time at which maximum is reached as well as the rate of FOS decomposition.",
publisher = "John Wiley and Sons Inc.",
journal = "AIChE Journal",
title = "Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products",
number = "9",
pages = "e18122",
volume = "69",
doi = "10.1002/aic.18122"
}

Pravilović, R., Janković, T., Veljković, M., Todić, B., Simović, M., Bezbradica, D.,& Nikačević, N.. (2023). Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products. in AIChE Journal
John Wiley and Sons Inc.., 69(9), e18122.
https://doi.org/10.1002/aic.18122

Pravilović R, Janković T, Veljković M, Todić B, Simović M, Bezbradica D, Nikačević N. Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products. in AIChE Journal. 2023;69(9):e18122.
doi:10.1002/aic.18122 .

Pravilović, Radoslava, Janković, Tamara, Veljković, Milica, Todić, Branislav, Simović, Milica, Bezbradica, Dejan, Nikačević, Nikola, "Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products" in AIChE Journal, 69, no. 9 (2023):e18122,
https://doi.org/10.1002/aic.18122 . .

TY  - CONF
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Petrov Ivanković, Anja
AU  - Nikolić, Valentina
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6842
AB  - Recently, there is an increasing interest in utilizing biomass and waste potential for development of new products and materials. The example of this is sunflower meal, by-product of sunflower seed oil industry, that is currently used as a cheap component of animal feed owing to a poor protein content. The major drawback for their application is high content of complex carbohydrates (15.27% hemicellulose, 12.54% cellulose and 5.88% lignin). Therefore, extraction and enzymatic transformation of carbohydrates (primarily xylan) represent an attractive valorization approach. Since xylan extraction from the plant cell wall is highly restricted by lignin presence, different alkaline extraction methods have been proposed. This work is focused on xylan extraction using ethanol extraction and subsequent delignification approach. The ethanol extraction step yielded fraction rich in simple sugars, polyphenols (predominantly chlorogenic acid) and colorants that proved to have great prebiotic potential for application in skin products enabling proliferation of beneficial skin commensal and suppressing the pathogen one. Introduction of additional delignification step enabled high xylan yields (91.35%). This fraction was subjected to the enzymatic treatment using commercial xylanase (ROHALASE® SEP-VISCO) to obtain xylo-oligosaccharides (XOS), compounds that comprise of 2–10 xylose residues linked via β-1,4-glycosidic bonds with high prebiotic activity and excellent application properties (high temperature and pH stability, good sweetening power and low caloric value) for food industry. As result of the sunflower meal xylan treatment under optimum reaction conditions (60°C, pH6 and enzyme concentration of 0.5% per substrate), XOS with a polymerization degree of 2–6 were produced (with predominance of XOS2), without xylose generation. The prebiotic activity of the obtained XOS fraction was confirmed by fermentation with beneficial microbial cultures and pathogenic gut microbiota representative. Therefore, it was shown that significant portion of meal can be converted to added-value functional ingredients, while remaining product retains higher protein content.
PB  - Belgrade : University, Faculty of Geography
C3  - Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023
T1  - Valorization of sunflower meal toward the production of emerging prebiotics
SP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6842
ER  - 

@conference{
author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Petrov Ivanković, Anja and Nikolić, Valentina and Bezbradica, Dejan",
year = "2023",
abstract = "Recently, there is an increasing interest in utilizing biomass and waste potential for development of new products and materials. The example of this is sunflower meal, by-product of sunflower seed oil industry, that is currently used as a cheap component of animal feed owing to a poor protein content. The major drawback for their application is high content of complex carbohydrates (15.27% hemicellulose, 12.54% cellulose and 5.88% lignin). Therefore, extraction and enzymatic transformation of carbohydrates (primarily xylan) represent an attractive valorization approach. Since xylan extraction from the plant cell wall is highly restricted by lignin presence, different alkaline extraction methods have been proposed. This work is focused on xylan extraction using ethanol extraction and subsequent delignification approach. The ethanol extraction step yielded fraction rich in simple sugars, polyphenols (predominantly chlorogenic acid) and colorants that proved to have great prebiotic potential for application in skin products enabling proliferation of beneficial skin commensal and suppressing the pathogen one. Introduction of additional delignification step enabled high xylan yields (91.35%). This fraction was subjected to the enzymatic treatment using commercial xylanase (ROHALASE® SEP-VISCO) to obtain xylo-oligosaccharides (XOS), compounds that comprise of 2–10 xylose residues linked via β-1,4-glycosidic bonds with high prebiotic activity and excellent application properties (high temperature and pH stability, good sweetening power and low caloric value) for food industry. As result of the sunflower meal xylan treatment under optimum reaction conditions (60°C, pH6 and enzyme concentration of 0.5% per substrate), XOS with a polymerization degree of 2–6 were produced (with predominance of XOS2), without xylose generation. The prebiotic activity of the obtained XOS fraction was confirmed by fermentation with beneficial microbial cultures and pathogenic gut microbiota representative. Therefore, it was shown that significant portion of meal can be converted to added-value functional ingredients, while remaining product retains higher protein content.",
publisher = "Belgrade : University, Faculty of Geography",
journal = "Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023",
title = "Valorization of sunflower meal toward the production of emerging prebiotics",
pages = "36",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6842"
}

Veljković, M., Simović, M., Banjanac, K., Petrov Ivanković, A., Nikolić, V.,& Bezbradica, D.. (2023). Valorization of sunflower meal toward the production of emerging prebiotics. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023
Belgrade : University, Faculty of Geography., 36.
https://hdl.handle.net/21.15107/rcub_technorep_6842

Veljković M, Simović M, Banjanac K, Petrov Ivanković A, Nikolić V, Bezbradica D. Valorization of sunflower meal toward the production of emerging prebiotics. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023. 2023;:36.
https://hdl.handle.net/21.15107/rcub_technorep_6842 .

Veljković, Milica, Simović, Milica, Banjanac, Katarina, Petrov Ivanković, Anja, Nikolić, Valentina, Bezbradica, Dejan, "Valorization of sunflower meal toward the production of emerging prebiotics" in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023 (2023):36,
https://hdl.handle.net/21.15107/rcub_technorep_6842 .

TY  - PAT
AU  - Simović, Milica
AU  - Bezbradica, Dejan
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5990
AB  - Predmetni pronalazak se odnosi na "in situ" enzimski
postupak sinteze frukto-oligosaharida direktnom
transfruktozilacijom saharoze prisutne u prehrambenim
proizvodima korišćenjem enzima fruktozil-transferaze. Na taj
način sharoza prisutna u proizvodu se konvertuje u
prebiotike, frukto-oligosaharide, pri čemu se dobija proizvod
poboljšanih funkcionalnih svojstava, sa smanjenim sadržajem
saharoze, tj. manjim glikemijskim indeksom. Takođe,
predmetni postupak se odnosi i na proizvode sa prebiotskom
aktivnošću proizvedene prema metodama ovog pronalaska
PB  - Гласник интелектуалне својине
T2  - Glasnik intelektualne svojine
T1  - Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics
IS  - 3
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5990
ER  - 

@misc{
author = "Simović, Milica and Bezbradica, Dejan and Ćorović, Marija and Milivojević, Ana and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "Predmetni pronalazak se odnosi na "in situ" enzimski
postupak sinteze frukto-oligosaharida direktnom
transfruktozilacijom saharoze prisutne u prehrambenim
proizvodima korišćenjem enzima fruktozil-transferaze. Na taj
način sharoza prisutna u proizvodu se konvertuje u
prebiotike, frukto-oligosaharide, pri čemu se dobija proizvod
poboljšanih funkcionalnih svojstava, sa smanjenim sadržajem
saharoze, tj. manjim glikemijskim indeksom. Takođe,
predmetni postupak se odnosi i na proizvode sa prebiotskom
aktivnošću proizvedene prema metodama ovog pronalaska",
publisher = "Гласник интелектуалне својине",
journal = "Glasnik intelektualne svojine",
title = "Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics",
number = "3",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5990"
}

Simović, M., Bezbradica, D., Ćorović, M., Milivojević, A.,& Dimitrijević-Branković, S.. (2022). Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics. in Glasnik intelektualne svojine
Гласник интелектуалне својине.(3), 8.
https://hdl.handle.net/21.15107/rcub_technorep_5990

Simović M, Bezbradica D, Ćorović M, Milivojević A, Dimitrijević-Branković S. Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics. in Glasnik intelektualne svojine. 2022;(3):8.
https://hdl.handle.net/21.15107/rcub_technorep_5990 .

Simović, Milica, Bezbradica, Dejan, Ćorović, Marija, Milivojević, Ana, Dimitrijević-Branković, Suzana, "Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics" in Glasnik intelektualne svojine, no. 3 (2022):8,
https://hdl.handle.net/21.15107/rcub_technorep_5990 .

TY  - CONF
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Petrov Ivanković, Anja
AU  - Vukoičić, Ana
AU  - Banjanac, Katarina
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5993
AB  - It is well known that maple syrup, a product obtained by processing maple tree sap, is widely
used in many households due to its sweetness and beneficial ingredients. It is most often used
as a topping for pancakes, waffles, donuts, and other desserts, but also as a sweetener for
numerous beverages such as coffee, tea, lemonade, and many others. This natural syrup, in
which, in addition to numerous minerals, antioxidants, carbohydrates and other components,
sucrose predominates, presents an excellent substrate for the synthesis of bioactive
molecules, i.e. fructo-oligosaccharides (FOS). These indigestible oligosaccharides which
belong to a group of established prebiotics are very suitable from the standpoint of human
health because they enable the normal functioning of the gastrointestinal tract, have a
positive effect on the immune system as well as many other benefits for the human organism.
With the approach which include the conversion of sucrose into FOS, it is possible to obtain a
product with improved characteristics, i.e. higher functional and lower caloric values.
Accordingly, in this study, a detailed optimization of the enzymatic synthesis of FOS was
performed using maple syrup as a source of sucrose and commercial enzyme mixture
Pectinex® Ultra SP-L as a source of fructosyltransferase. Namely, by individual varying of
enzymatic synthesis key factors such as temperature (30-80 °C), enzyme concentration (1, 3
and 5%) and reaction time (0-25 h), optimal conditions were selected. It was determined that
by performing the synthesis reaction at a temperature of 60 °C with an enzyme concentration
of 3% for 12h, a remarkable sucrose hydrolysis degree of 82% and a FOS yield of approximately 55% were achieved. In this case, the estimated caloric value of the obtained
product is about 2.2 kcal/mL, which is around 1.6-fold lower compared to the initial value of
3.6 kcal/mL. The product obtained in this manner could represent a low-calorie sweetener
with high fiber content and could potentially be included in the diet of diabetics and obese
people, as well as all those who aim to maintain better overall health
PB  - Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia
C3  - 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts
T1  - In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product
EP  - 153
SP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5993
ER  - 

@conference{
author = "Veljković, Milica and Simović, Milica and Petrov Ivanković, Anja and Vukoičić, Ana and Banjanac, Katarina and Bezbradica, Dejan",
year = "2022",
abstract = "It is well known that maple syrup, a product obtained by processing maple tree sap, is widely
used in many households due to its sweetness and beneficial ingredients. It is most often used
as a topping for pancakes, waffles, donuts, and other desserts, but also as a sweetener for
numerous beverages such as coffee, tea, lemonade, and many others. This natural syrup, in
which, in addition to numerous minerals, antioxidants, carbohydrates and other components,
sucrose predominates, presents an excellent substrate for the synthesis of bioactive
molecules, i.e. fructo-oligosaccharides (FOS). These indigestible oligosaccharides which
belong to a group of established prebiotics are very suitable from the standpoint of human
health because they enable the normal functioning of the gastrointestinal tract, have a
positive effect on the immune system as well as many other benefits for the human organism.
With the approach which include the conversion of sucrose into FOS, it is possible to obtain a
product with improved characteristics, i.e. higher functional and lower caloric values.
Accordingly, in this study, a detailed optimization of the enzymatic synthesis of FOS was
performed using maple syrup as a source of sucrose and commercial enzyme mixture
Pectinex® Ultra SP-L as a source of fructosyltransferase. Namely, by individual varying of
enzymatic synthesis key factors such as temperature (30-80 °C), enzyme concentration (1, 3
and 5%) and reaction time (0-25 h), optimal conditions were selected. It was determined that
by performing the synthesis reaction at a temperature of 60 °C with an enzyme concentration
of 3% for 12h, a remarkable sucrose hydrolysis degree of 82% and a FOS yield of approximately 55% were achieved. In this case, the estimated caloric value of the obtained
product is about 2.2 kcal/mL, which is around 1.6-fold lower compared to the initial value of
3.6 kcal/mL. The product obtained in this manner could represent a low-calorie sweetener
with high fiber content and could potentially be included in the diet of diabetics and obese
people, as well as all those who aim to maintain better overall health",
publisher = "Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia",
journal = "10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts",
title = "In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product",
pages = "153-152",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5993"
}

Veljković, M., Simović, M., Petrov Ivanković, A., Vukoičić, A., Banjanac, K.,& Bezbradica, D.. (2022). In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product. in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts
Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia., 152-153.
https://hdl.handle.net/21.15107/rcub_technorep_5993

Veljković M, Simović M, Petrov Ivanković A, Vukoičić A, Banjanac K, Bezbradica D. In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product. in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts. 2022;:152-153.
https://hdl.handle.net/21.15107/rcub_technorep_5993 .

Veljković, Milica, Simović, Milica, Petrov Ivanković, Anja, Vukoičić, Ana, Banjanac, Katarina, Bezbradica, Dejan, "In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product" in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts (2022):152-153,
https://hdl.handle.net/21.15107/rcub_technorep_5993 .

Simović, M., Veljković, M., Ćorović, M., Milivojević, A., Banjanac, K.,& Veljović, M.. (2022). Modifikacija nadeva bundeve direktnom enzimskom sintezom frukto-oligosaharida u cilju obogaćivanja proizvoda prebioticima. .
https://hdl.handle.net/21.15107/rcub_technorep_6014

Simović M, Veljković M, Ćorović M, Milivojević A, Banjanac K, Veljović M. Modifikacija nadeva bundeve direktnom enzimskom sintezom frukto-oligosaharida u cilju obogaćivanja proizvoda prebioticima. 2022;.
https://hdl.handle.net/21.15107/rcub_technorep_6014 .

Simović, Milica, Veljković, Milica, Ćorović, Marija, Milivojević, Ana, Banjanac, Katarina, Veljović, Mile, "Modifikacija nadeva bundeve direktnom enzimskom sintezom frukto-oligosaharida u cilju obogaćivanja proizvoda prebioticima" (2022),
https://hdl.handle.net/21.15107/rcub_technorep_6014 .

TY  - JOUR
AU  - Petrov, Anja
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5141
AB  - Recently, prebiotics are attracting plenty of attention in the field of skin care, since it is found that they are able to support the balance of beneficial and harmful microorganisms on the skin, and accordingly prevent several skin conditions associated with microbial imbalance. Topical application of prebiotics, although insufficiently investigated, holds great promise in improving skin health. The purpose of this research was to determine the prebiotic potential of galacto-oligosaccharides (GOS) for skin microbiota and suitability for incorporation in different topical formulations, and finally, provide insights into their diffusion properties. Methods: The prebiotic effect of GOS was evaluated through the influence on the growth of Staphylococcus epidermidis and Staphylococcus aureus, the most common resident and pathogenic bacterium of the skin microbiota, respectively. Also, with the future use of GOS in cosmetic products in mind, the diffusion of GOS molecules from two different topical formulations, hydrogel and oil-in-water (O/W) gel emulsion, was monitored employing Franz diffusion cell and two systems—with cellulose acetate membrane and transdermal diffusion test model, Strat-M® membrane. Course of fermentation and the amount of diffused GOS molecules were monitored using high-performance liquid chromatography (HPLC). Results: The in vitro results revealed that GOS at a concentration of 5% (w/v) has a pronounced stimulatory effect on S. epidermidis, while simultaneously showing an inhibitory effect on S. aureus, both in nutrient broth and cosmetic formulations. GOS trisaccharide and tetrasaccharide diffusion coefficients from O/W gel emulsion were calculated to be 5.61·10−6 cm2 s−1 and 1.41·10−8 cm2 s−1, respectively. The diffusion coefficient of GOS trisaccharides from hydrogel was 3.22·10−6 cm2 s−1, while it was not determined for tetrasaccharides due to low diffused concentration. Transdermal diffusion tests revealed that GOS incorporated in two formulations stays at the surface of the skin even after 24 h. Conclusion: When applied in adequate concentration, GOS has the potential to be used as a skin prebiotic. Novel GOS enriched formulations, Aristoflex® AVC-based hydrogel and Heliogel™-based O/W gel emulsion, provided efficient diffusion and delivery of prebiotic GOS molecules to the skin surface.
PB  - John Wiley and Sons Inc.
T2  - International Journal of Cosmetic Science
T1  - Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties
EP  - 319
IS  - 3
SP  - 309
VL  - 44
DO  - 10.1111/ics.12778
ER  - 

@article{
author = "Petrov, Anja and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Banjanac, Katarina and Pjanović, Rada and Bezbradica, Dejan",
year = "2022",
abstract = "Recently, prebiotics are attracting plenty of attention in the field of skin care, since it is found that they are able to support the balance of beneficial and harmful microorganisms on the skin, and accordingly prevent several skin conditions associated with microbial imbalance. Topical application of prebiotics, although insufficiently investigated, holds great promise in improving skin health. The purpose of this research was to determine the prebiotic potential of galacto-oligosaccharides (GOS) for skin microbiota and suitability for incorporation in different topical formulations, and finally, provide insights into their diffusion properties. Methods: The prebiotic effect of GOS was evaluated through the influence on the growth of Staphylococcus epidermidis and Staphylococcus aureus, the most common resident and pathogenic bacterium of the skin microbiota, respectively. Also, with the future use of GOS in cosmetic products in mind, the diffusion of GOS molecules from two different topical formulations, hydrogel and oil-in-water (O/W) gel emulsion, was monitored employing Franz diffusion cell and two systems—with cellulose acetate membrane and transdermal diffusion test model, Strat-M® membrane. Course of fermentation and the amount of diffused GOS molecules were monitored using high-performance liquid chromatography (HPLC). Results: The in vitro results revealed that GOS at a concentration of 5% (w/v) has a pronounced stimulatory effect on S. epidermidis, while simultaneously showing an inhibitory effect on S. aureus, both in nutrient broth and cosmetic formulations. GOS trisaccharide and tetrasaccharide diffusion coefficients from O/W gel emulsion were calculated to be 5.61·10−6 cm2 s−1 and 1.41·10−8 cm2 s−1, respectively. The diffusion coefficient of GOS trisaccharides from hydrogel was 3.22·10−6 cm2 s−1, while it was not determined for tetrasaccharides due to low diffused concentration. Transdermal diffusion tests revealed that GOS incorporated in two formulations stays at the surface of the skin even after 24 h. Conclusion: When applied in adequate concentration, GOS has the potential to be used as a skin prebiotic. Novel GOS enriched formulations, Aristoflex® AVC-based hydrogel and Heliogel™-based O/W gel emulsion, provided efficient diffusion and delivery of prebiotic GOS molecules to the skin surface.",
publisher = "John Wiley and Sons Inc.",
journal = "International Journal of Cosmetic Science",
title = "Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties",
pages = "319-309",
number = "3",
volume = "44",
doi = "10.1111/ics.12778"
}

Petrov, A., Ćorović, M., Milivojević, A., Simović, M., Banjanac, K., Pjanović, R.,& Bezbradica, D.. (2022). Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties. in International Journal of Cosmetic Science
John Wiley and Sons Inc.., 44(3), 309-319.
https://doi.org/10.1111/ics.12778

Petrov A, Ćorović M, Milivojević A, Simović M, Banjanac K, Pjanović R, Bezbradica D. Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties. in International Journal of Cosmetic Science. 2022;44(3):309-319.
doi:10.1111/ics.12778 .

Petrov, Anja, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Banjanac, Katarina, Pjanović, Rada, Bezbradica, Dejan, "Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties" in International Journal of Cosmetic Science, 44, no. 3 (2022):309-319,
https://doi.org/10.1111/ics.12778 . .

TY  - JOUR
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5203
AB  - This work aims to evaluate the effect of cosmetic formulations on the skin permeation of phloridzin acetates, therefore in vitro transdermal delivery of these bioactive compounds across Strat-M membrane is investigated. The formulations tested are gel emulsion, oil in water emulsion (O/W emulsion), gel in oil emulsion, liquid crystal emulsion, and liposomes dispersed in gel. The release of esters from the O/W emulsion is diffusion-controlled (n = 0.5), while release kinetics from gel-based formulations depends on coupled diffusion and polymer relaxations effect (0.5 < n < 1). The highest values of effective diffusion coefficient are obtained after release from O/W emulsion (24.64 × 10−10 m2 min−1 for phloridzin acetate and 6.55 × 10−10 m2 min−1 for phloridzin diacetate). The lag phase lasting from 1 h (gel emulsion) to 5 h (liquid crystal emulsion) indicates that esters permeation is significantly affected by the formulation used. Formulations in which the water is continuous phase are the most appropriate carrier system for transdermal delivery of phloridzin esters. Additionally, liposomes are successfully applied as carrier systems for the controlled release of esters, although it should be noted that liposomes without cholesterol show remarkable stability (ζ = −43 mV) and homogeneity (polydispersity index = 0.198). Practical application: Phloridzin is a flavonoid glycoside that possesses many beneficial activities such as antioxidant, UV-protecting, and anti-inflammatory activity, so the use of this flavonoid and its derivatives in numerous cosmetic and pharmaceutical formulations seems very attractive. Results obtained in this study give a comprehensive insight into the release mechanism of phloridzin acetates from emulsions and liposomes, enable the selection of optimum cosmetic formulations depending on the application, and provide significant scientific contribution in the field of topical administration of bioactive flavonoid derivatives.
PB  - John Wiley and Sons Inc.
T2  - European Journal of Lipid Science and Technology
T1  - Evaluation of In Vitro Skin Permeation of Enzymatically Synthesized Phloridzin Acetates from Emulsions and Liposomes Dispersed in Gel
IS  - 10
SP  - 2200073
VL  - 124
DO  - 10.1002/ejlt.202200073
ER  - 

@article{
author = "Milivojević, Ana and Ćorović, Marija and Simović, Milica and Banjanac, Katarina and Pjanović, Rada and Bezbradica, Dejan",
year = "2022",
abstract = "This work aims to evaluate the effect of cosmetic formulations on the skin permeation of phloridzin acetates, therefore in vitro transdermal delivery of these bioactive compounds across Strat-M membrane is investigated. The formulations tested are gel emulsion, oil in water emulsion (O/W emulsion), gel in oil emulsion, liquid crystal emulsion, and liposomes dispersed in gel. The release of esters from the O/W emulsion is diffusion-controlled (n = 0.5), while release kinetics from gel-based formulations depends on coupled diffusion and polymer relaxations effect (0.5 < n < 1). The highest values of effective diffusion coefficient are obtained after release from O/W emulsion (24.64 × 10−10 m2 min−1 for phloridzin acetate and 6.55 × 10−10 m2 min−1 for phloridzin diacetate). The lag phase lasting from 1 h (gel emulsion) to 5 h (liquid crystal emulsion) indicates that esters permeation is significantly affected by the formulation used. Formulations in which the water is continuous phase are the most appropriate carrier system for transdermal delivery of phloridzin esters. Additionally, liposomes are successfully applied as carrier systems for the controlled release of esters, although it should be noted that liposomes without cholesterol show remarkable stability (ζ = −43 mV) and homogeneity (polydispersity index = 0.198). Practical application: Phloridzin is a flavonoid glycoside that possesses many beneficial activities such as antioxidant, UV-protecting, and anti-inflammatory activity, so the use of this flavonoid and its derivatives in numerous cosmetic and pharmaceutical formulations seems very attractive. Results obtained in this study give a comprehensive insight into the release mechanism of phloridzin acetates from emulsions and liposomes, enable the selection of optimum cosmetic formulations depending on the application, and provide significant scientific contribution in the field of topical administration of bioactive flavonoid derivatives.",
publisher = "John Wiley and Sons Inc.",
journal = "European Journal of Lipid Science and Technology",
title = "Evaluation of In Vitro Skin Permeation of Enzymatically Synthesized Phloridzin Acetates from Emulsions and Liposomes Dispersed in Gel",
number = "10",
pages = "2200073",
volume = "124",
doi = "10.1002/ejlt.202200073"
}

Milivojević, A., Ćorović, M., Simović, M., Banjanac, K., Pjanović, R.,& Bezbradica, D.. (2022). Evaluation of In Vitro Skin Permeation of Enzymatically Synthesized Phloridzin Acetates from Emulsions and Liposomes Dispersed in Gel. in European Journal of Lipid Science and Technology
John Wiley and Sons Inc.., 124(10), 2200073.
https://doi.org/10.1002/ejlt.202200073

Milivojević A, Ćorović M, Simović M, Banjanac K, Pjanović R, Bezbradica D. Evaluation of In Vitro Skin Permeation of Enzymatically Synthesized Phloridzin Acetates from Emulsions and Liposomes Dispersed in Gel. in European Journal of Lipid Science and Technology. 2022;124(10):2200073.
doi:10.1002/ejlt.202200073 .

Milivojević, Ana, Ćorović, Marija, Simović, Milica, Banjanac, Katarina, Pjanović, Rada, Bezbradica, Dejan, "Evaluation of In Vitro Skin Permeation of Enzymatically Synthesized Phloridzin Acetates from Emulsions and Liposomes Dispersed in Gel" in European Journal of Lipid Science and Technology, 124, no. 10 (2022):2200073,
https://doi.org/10.1002/ejlt.202200073 . .

TY  - JOUR
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Milivojević, Milan
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5281
AB  - In this study, a heterofunctional carrier was obtained by modification of a macroporous polystyrene ion exchange resin with primary amino groups (Purolite® A109) with epichlorohydrin, in view of achieving covalent immobilization of fructosyltransferase (FTase) from a complex enzyme mixture, Pectinex® Ultra SP-L, that is responsible for the synthesis of functionally active fructo-oligosaccharides (FOS). A two-step immobilization protocol, comprising the physical adsorption of FTase performed at pH 4 and subsequent buffer exchange to promote the establishment of covalent bonds at pH 9, was proposed for obtaining a highly active immobilized preparation (243 IU g−1 of support). Additionally, this protocol provided development of a preparation with 20 times more prominent expressed activity of FTase compared to the commercial preparation with predominant pectinase activity. The obtained immobilized preparation was further tested in batch and air-lift reactor systems for FOS synthesis, yielding 52.8% and 54.7% of FOS in total carbohydrates, respectively. Finally, the continuous production of FOS in the air-lift reactor was established for 7 days, with an average FOS yield of 52.5%. Accordingly, it is demonstrated that the immobilization process enabled the development of preparations with exceptional potential for industrial implementation.
PB  - Royal Society of Chemistry
T2  - Reaction Chemistry and Engineering
T1  - Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides
EP  - 2526
IS  - 12
SP  - 2518
VL  - 7
DO  - 10.1039/d2re00182a
ER  - 

@article{
author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Ćorović, Marija and Milivojević, Ana and Milivojević, Milan and Bezbradica, Dejan",
year = "2022",
abstract = "In this study, a heterofunctional carrier was obtained by modification of a macroporous polystyrene ion exchange resin with primary amino groups (Purolite® A109) with epichlorohydrin, in view of achieving covalent immobilization of fructosyltransferase (FTase) from a complex enzyme mixture, Pectinex® Ultra SP-L, that is responsible for the synthesis of functionally active fructo-oligosaccharides (FOS). A two-step immobilization protocol, comprising the physical adsorption of FTase performed at pH 4 and subsequent buffer exchange to promote the establishment of covalent bonds at pH 9, was proposed for obtaining a highly active immobilized preparation (243 IU g−1 of support). Additionally, this protocol provided development of a preparation with 20 times more prominent expressed activity of FTase compared to the commercial preparation with predominant pectinase activity. The obtained immobilized preparation was further tested in batch and air-lift reactor systems for FOS synthesis, yielding 52.8% and 54.7% of FOS in total carbohydrates, respectively. Finally, the continuous production of FOS in the air-lift reactor was established for 7 days, with an average FOS yield of 52.5%. Accordingly, it is demonstrated that the immobilization process enabled the development of preparations with exceptional potential for industrial implementation.",
publisher = "Royal Society of Chemistry",
journal = "Reaction Chemistry and Engineering",
title = "Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides",
pages = "2526-2518",
number = "12",
volume = "7",
doi = "10.1039/d2re00182a"
}

Veljković, M., Simović, M., Banjanac, K., Ćorović, M., Milivojević, A., Milivojević, M.,& Bezbradica, D.. (2022). Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides. in Reaction Chemistry and Engineering
Royal Society of Chemistry., 7(12), 2518-2526.
https://doi.org/10.1039/d2re00182a

Veljković M, Simović M, Banjanac K, Ćorović M, Milivojević A, Milivojević M, Bezbradica D. Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides. in Reaction Chemistry and Engineering. 2022;7(12):2518-2526.
doi:10.1039/d2re00182a .

Veljković, Milica, Simović, Milica, Banjanac, Katarina, Ćorović, Marija, Milivojević, Ana, Milivojević, Milan, Bezbradica, Dejan, "Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides" in Reaction Chemistry and Engineering, 7, no. 12 (2022):2518-2526,
https://doi.org/10.1039/d2re00182a . .

TY  - CONF
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Petrov Ivanković, Anja
AU  - Banjanac, Katarina
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5995
AB  - In recent decades, the scientific community has recognized the growing importance of prebiotics, and therefore, numerous studies are focused on their economical production due to their low presence in natural resources. It has been confirmed that prebiotics are a source of energy for probiotics in the gastrointestinal tract (GIT) and enable their proliferation, consequently leading to the normal functioning of the intestinal microbiota. Also, products of their fermentation are short-chain fatty acids (SCFA), which play a key role in maintaining and improving the health not only of the GIT but also of the whole organism. Among several confirmed prebiotics, fructo-oligosaccharides (FOS) are considered interesting candidates for use in a wide range of products in the food industry. They are characterized as low-calorie and non-cariogenic substances that represent an adequate sugar substitute and can be considered suitable for use in products intended for diabetics. The subject of this research will be the production of FOS by transforming sucrose using a fructosyltransferase (FTase) present in commercial preparation Pectinex® Ultra SP-L, with special emphasis on the development of adequate FTase immobilization method that would enable selective isolation of the enzyme responsible for the synthesis of FOS from the complex enzymatic mixture. This would lead to considerable enzyme purification and allow its direct incorporation into different sucrosebased products, without the fear that action of the other hydrolytic enzymes may adversely affect the products functional characteristics. Accordingly, the possibility of selective immobilization of the enzyme using support with primary amino groups, Purolite® A109, which was previously activated and modified using glutaraldehyde (GA), was investigated. In the initial phase of the research, the effects of individual immobilization parameters such as pH, enzyme concentration and immobilization time were investigated to optimize the process using support chemically activated with 15% and 0.5% GA to form dimers and monomers, respectively. It was determined that highly active immobilized preparations (371.8 IU/g of support - dimer and 213.8 IU/g of support – monomer) were achieved under acidic conditions (pH 4) provided that an enzyme concentration was 50 mg/g of support after 7 h and 3 h, respectively. Bearing in mind the obtained results of the expressed activity, it is noticeable that the formation of dimers showed higher reactivity compared to the form of monomers. Also, in the case of support modification using 15% GA, the value of the ratio of FTase and pectinase (as dominant enzyme mixture component) activity immobilization yields was 16.45, indicating the high feasibility of selective immobilization of FTase on modified polystyrene resin. After obtaining immobilized preparations of satisfactory features, they were tested in a reaction of FOS synthesis under determined optimal conditions. The maximum FOS yields of approximately 50% of total carbohydrates in the reaction mixture were recorded after 21 h. Finally, it can be concluded that the examined immobilization method yielded highly active, stable and more importantly refined enzyme preparation that can be further utilized on a larger scale for development of continual processes for FOS synthesis, as well as for modification of different sucrose-based mediums.
PB  - The world academy of science, engineering and technology
C3  - XVI. international conference on advances in probiotics and prebiotics, Copenhagen Denmark, Proceedings
T1  - Selective Immobilization of Fructosyltransferase onto Glutaraldehyde Modified Support and Its Application in the Production of Fructo-oligosaccharides
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5995
ER  - 

@conference{
author = "Veljković, Milica and Simović, Milica and Ćorović, Marija and Milivojević, Ana and Petrov Ivanković, Anja and Banjanac, Katarina and Bezbradica, Dejan",
year = "2022",
abstract = "In recent decades, the scientific community has recognized the growing importance of prebiotics, and therefore, numerous studies are focused on their economical production due to their low presence in natural resources. It has been confirmed that prebiotics are a source of energy for probiotics in the gastrointestinal tract (GIT) and enable their proliferation, consequently leading to the normal functioning of the intestinal microbiota. Also, products of their fermentation are short-chain fatty acids (SCFA), which play a key role in maintaining and improving the health not only of the GIT but also of the whole organism. Among several confirmed prebiotics, fructo-oligosaccharides (FOS) are considered interesting candidates for use in a wide range of products in the food industry. They are characterized as low-calorie and non-cariogenic substances that represent an adequate sugar substitute and can be considered suitable for use in products intended for diabetics. The subject of this research will be the production of FOS by transforming sucrose using a fructosyltransferase (FTase) present in commercial preparation Pectinex® Ultra SP-L, with special emphasis on the development of adequate FTase immobilization method that would enable selective isolation of the enzyme responsible for the synthesis of FOS from the complex enzymatic mixture. This would lead to considerable enzyme purification and allow its direct incorporation into different sucrosebased products, without the fear that action of the other hydrolytic enzymes may adversely affect the products functional characteristics. Accordingly, the possibility of selective immobilization of the enzyme using support with primary amino groups, Purolite® A109, which was previously activated and modified using glutaraldehyde (GA), was investigated. In the initial phase of the research, the effects of individual immobilization parameters such as pH, enzyme concentration and immobilization time were investigated to optimize the process using support chemically activated with 15% and 0.5% GA to form dimers and monomers, respectively. It was determined that highly active immobilized preparations (371.8 IU/g of support - dimer and 213.8 IU/g of support – monomer) were achieved under acidic conditions (pH 4) provided that an enzyme concentration was 50 mg/g of support after 7 h and 3 h, respectively. Bearing in mind the obtained results of the expressed activity, it is noticeable that the formation of dimers showed higher reactivity compared to the form of monomers. Also, in the case of support modification using 15% GA, the value of the ratio of FTase and pectinase (as dominant enzyme mixture component) activity immobilization yields was 16.45, indicating the high feasibility of selective immobilization of FTase on modified polystyrene resin. After obtaining immobilized preparations of satisfactory features, they were tested in a reaction of FOS synthesis under determined optimal conditions. The maximum FOS yields of approximately 50% of total carbohydrates in the reaction mixture were recorded after 21 h. Finally, it can be concluded that the examined immobilization method yielded highly active, stable and more importantly refined enzyme preparation that can be further utilized on a larger scale for development of continual processes for FOS synthesis, as well as for modification of different sucrose-based mediums.",
publisher = "The world academy of science, engineering and technology",
journal = "XVI. international conference on advances in probiotics and prebiotics, Copenhagen Denmark, Proceedings",
title = "Selective Immobilization of Fructosyltransferase onto Glutaraldehyde Modified Support and Its Application in the Production of Fructo-oligosaccharides",
pages = "83",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5995"
}

Veljković, M., Simović, M., Ćorović, M., Milivojević, A., Petrov Ivanković, A., Banjanac, K.,& Bezbradica, D.. (2022). Selective Immobilization of Fructosyltransferase onto Glutaraldehyde Modified Support and Its Application in the Production of Fructo-oligosaccharides. in XVI. international conference on advances in probiotics and prebiotics, Copenhagen Denmark, Proceedings
The world academy of science, engineering and technology., 83.
https://hdl.handle.net/21.15107/rcub_technorep_5995

Veljković M, Simović M, Ćorović M, Milivojević A, Petrov Ivanković A, Banjanac K, Bezbradica D. Selective Immobilization of Fructosyltransferase onto Glutaraldehyde Modified Support and Its Application in the Production of Fructo-oligosaccharides. in XVI. international conference on advances in probiotics and prebiotics, Copenhagen Denmark, Proceedings. 2022;:83.
https://hdl.handle.net/21.15107/rcub_technorep_5995 .

Veljković, Milica, Simović, Milica, Ćorović, Marija, Milivojević, Ana, Petrov Ivanković, Anja, Banjanac, Katarina, Bezbradica, Dejan, "Selective Immobilization of Fructosyltransferase onto Glutaraldehyde Modified Support and Its Application in the Production of Fructo-oligosaccharides" in XVI. international conference on advances in probiotics and prebiotics, Copenhagen Denmark, Proceedings (2022):83,
https://hdl.handle.net/21.15107/rcub_technorep_5995 .

TY  - CONF
AU  - Petrov Ivanković, Anja
AU  - Veljković, Milica
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5996
AB  - One of the fundamental requirements for overall human well-being is a stable and balanced microbiome. Aside from the microorganisms that reside within the body, a large number of microorganisms, especially bacteria, swarming the human skin are in homeostasis with the host and represent a skin microbiota. Even though the immune system of the skin is capable of distinguishing between commensal and potentially harmful transient bacteria, the cutaneous microbial balance can be disrupted under certain circumstances. In that case, reduction in the skin microbiota diversity, as well as changes in metabolic activity, result in dermal infections and inflammation. Probiotics and prebiotics have the potential to play a significant role in the treatment of these skin disorders. The most common resident bacteria found on the skin, Staphylococcus epidermidis, can act as a potential skin probiotic, contributing to the protection of healthy skin from pathogen colonization, such as Staphylococcus aureus, which is related to atopic dermatitis exacerbation. However, as it is difficult to meet regulations in cosmetic products, another therapy approach could be topical prebiotic supplementation of the skin microbiota. In recent research, polyphenols are attracting scientists' interest as biomolecules with possible prebiotic effects on the skin microbiota. This research aimed to determine how herbal extracts rich in different polyphenolic compounds (lemon balm, St. John's wort, coltsfoot, pine needle and yarrow) affected the growth of S. epidermidis and S. aureus. The first part of the study involved screening of plants to determine if they could be regarded as probable candidates to be skin prebiotics. The effect of each plant on bacterial growth was examined by supplementing the nutrient medium with their extracts and comparing it with control samples (without extract). The results obtained after 24 h of incubation showed that all tested extracts influenced the growth of the examined bacteria to some extent. Since lemon balm and St. John's wort extracts displayed bactericidal activity against S. epidermidis, whereas coltsfoot inhibited both bacteria equally, they were not explored further. On the other hand, pine needle and yarrow extract led to an increase in S. epidermidis/S. aureus ratio, making them prospective candidates to be used as skin prebiotics. Byexamining the prebiotic effect of two extracts at different concentrations, it was revealed that, in the case of yarrow, 0.1% of extract dry matter in the fermentation medium was optimal, while for the pine needle extract, a concentration of 0.05% was preferred, since it selectively stimulated S. epidermidis growth and inhibited S. aureus proliferation. Additionally, total polyphenols and flavonoids content of two extracts were determined, revealing different concentrations and polyphenol profiles. Since yarrow and pine extracts affected the growth of skin bacteria in a dose-dependent manner, by carefully selecting the quantities of these extracts, and thus polyphenols content, it is possible to achieve desirable alterations of skin microbiota composition, which may be suitable for the treatment of atopic dermatitis.
PB  - The world academy of science, engineering and technology
C3  - XVI. international conference on advances in probiotics and prebiotics, Copenhagen Denmark, Proceedings
T1  - Evaluation of Herbal Extracts for Their Potential Application as Skin Prebiotics
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5996
ER  - 

@conference{
author = "Petrov Ivanković, Anja and Veljković, Milica and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Banjanac, Katarina and Bezbradica, Dejan",
year = "2022",
abstract = "One of the fundamental requirements for overall human well-being is a stable and balanced microbiome. Aside from the microorganisms that reside within the body, a large number of microorganisms, especially bacteria, swarming the human skin are in homeostasis with the host and represent a skin microbiota. Even though the immune system of the skin is capable of distinguishing between commensal and potentially harmful transient bacteria, the cutaneous microbial balance can be disrupted under certain circumstances. In that case, reduction in the skin microbiota diversity, as well as changes in metabolic activity, result in dermal infections and inflammation. Probiotics and prebiotics have the potential to play a significant role in the treatment of these skin disorders. The most common resident bacteria found on the skin, Staphylococcus epidermidis, can act as a potential skin probiotic, contributing to the protection of healthy skin from pathogen colonization, such as Staphylococcus aureus, which is related to atopic dermatitis exacerbation. However, as it is difficult to meet regulations in cosmetic products, another therapy approach could be topical prebiotic supplementation of the skin microbiota. In recent research, polyphenols are attracting scientists' interest as biomolecules with possible prebiotic effects on the skin microbiota. This research aimed to determine how herbal extracts rich in different polyphenolic compounds (lemon balm, St. John's wort, coltsfoot, pine needle and yarrow) affected the growth of S. epidermidis and S. aureus. The first part of the study involved screening of plants to determine if they could be regarded as probable candidates to be skin prebiotics. The effect of each plant on bacterial growth was examined by supplementing the nutrient medium with their extracts and comparing it with control samples (without extract). The results obtained after 24 h of incubation showed that all tested extracts influenced the growth of the examined bacteria to some extent. Since lemon balm and St. John's wort extracts displayed bactericidal activity against S. epidermidis, whereas coltsfoot inhibited both bacteria equally, they were not explored further. On the other hand, pine needle and yarrow extract led to an increase in S. epidermidis/S. aureus ratio, making them prospective candidates to be used as skin prebiotics. Byexamining the prebiotic effect of two extracts at different concentrations, it was revealed that, in the case of yarrow, 0.1% of extract dry matter in the fermentation medium was optimal, while for the pine needle extract, a concentration of 0.05% was preferred, since it selectively stimulated S. epidermidis growth and inhibited S. aureus proliferation. Additionally, total polyphenols and flavonoids content of two extracts were determined, revealing different concentrations and polyphenol profiles. Since yarrow and pine extracts affected the growth of skin bacteria in a dose-dependent manner, by carefully selecting the quantities of these extracts, and thus polyphenols content, it is possible to achieve desirable alterations of skin microbiota composition, which may be suitable for the treatment of atopic dermatitis.",
publisher = "The world academy of science, engineering and technology",
journal = "XVI. international conference on advances in probiotics and prebiotics, Copenhagen Denmark, Proceedings",
title = "Evaluation of Herbal Extracts for Their Potential Application as Skin Prebiotics",
pages = "84",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5996"
}

Petrov Ivanković, A., Veljković, M., Ćorović, M., Milivojević, A., Simović, M., Banjanac, K.,& Bezbradica, D.. (2022). Evaluation of Herbal Extracts for Their Potential Application as Skin Prebiotics. in XVI. international conference on advances in probiotics and prebiotics, Copenhagen Denmark, Proceedings
The world academy of science, engineering and technology., 84.
https://hdl.handle.net/21.15107/rcub_technorep_5996

Petrov Ivanković A, Veljković M, Ćorović M, Milivojević A, Simović M, Banjanac K, Bezbradica D. Evaluation of Herbal Extracts for Their Potential Application as Skin Prebiotics. in XVI. international conference on advances in probiotics and prebiotics, Copenhagen Denmark, Proceedings. 2022;:84.
https://hdl.handle.net/21.15107/rcub_technorep_5996 .

Petrov Ivanković, Anja, Veljković, Milica, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Banjanac, Katarina, Bezbradica, Dejan, "Evaluation of Herbal Extracts for Their Potential Application as Skin Prebiotics" in XVI. international conference on advances in probiotics and prebiotics, Copenhagen Denmark, Proceedings (2022):84,
https://hdl.handle.net/21.15107/rcub_technorep_5996 .

TY  - JOUR
AU  - Pravilović, Radoslava
AU  - Todić, Branislav
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Bezbradica, Dejan
AU  - Nikačević, Nikola
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5226
AB  - A new micro-kinetic model is proposed for galacto-oligosaccharide (GOS) synthesis with β-galactosidase from Aspergillus oryzae. Several kinetic models with similar reaction mechanisms, i.e., steps and kinetic constants, have been investigated covering a range of initial lactose (20-40 wt %) and enzyme concentrations (0.5-2 mg/mL). The kinetic parameters were estimated simultaneously for all experimental results using a hybrid genetic algorithm. A detailed analysis of the effect of process parameters on the concentrations of lactose and GOS is performed. The proposed model has a very good quantitative fit of the experimental data, with an average error of 9.34%. More importantly, qualitative trends for the change in the concentration of lactose and GOS are predicted excellently, especially if one takes into account the variation of both concentrations of enzyme and lactose in a range wider than reported in the literature to date. Also, the analysis between the two comparative reactions of transglycosylation and lactose hydrolysis confirmed the positive effect of increasing lactose concentration on the production of GOS. On the other hand, a higher enzyme concentration resulted in a faster production of GOS but with more intensive decomposition at longer reaction times.
PB  - American Chemical Society
T2  - Industrial and Engineering Chemistry Research
T1  - Kinetic Model for Galacto-Oligosaccharide Synthesis
EP  - 14198
IS  - 38
SP  - 14189
VL  - 61
DO  - 10.1021/acs.iecr.2c02053
ER  - 

@article{
author = "Pravilović, Radoslava and Todić, Branislav and Simović, Milica and Banjanac, Katarina and Bezbradica, Dejan and Nikačević, Nikola",
year = "2022",
abstract = "A new micro-kinetic model is proposed for galacto-oligosaccharide (GOS) synthesis with β-galactosidase from Aspergillus oryzae. Several kinetic models with similar reaction mechanisms, i.e., steps and kinetic constants, have been investigated covering a range of initial lactose (20-40 wt %) and enzyme concentrations (0.5-2 mg/mL). The kinetic parameters were estimated simultaneously for all experimental results using a hybrid genetic algorithm. A detailed analysis of the effect of process parameters on the concentrations of lactose and GOS is performed. The proposed model has a very good quantitative fit of the experimental data, with an average error of 9.34%. More importantly, qualitative trends for the change in the concentration of lactose and GOS are predicted excellently, especially if one takes into account the variation of both concentrations of enzyme and lactose in a range wider than reported in the literature to date. Also, the analysis between the two comparative reactions of transglycosylation and lactose hydrolysis confirmed the positive effect of increasing lactose concentration on the production of GOS. On the other hand, a higher enzyme concentration resulted in a faster production of GOS but with more intensive decomposition at longer reaction times.",
publisher = "American Chemical Society",
journal = "Industrial and Engineering Chemistry Research",
title = "Kinetic Model for Galacto-Oligosaccharide Synthesis",
pages = "14198-14189",
number = "38",
volume = "61",
doi = "10.1021/acs.iecr.2c02053"
}

Pravilović, R., Todić, B., Simović, M., Banjanac, K., Bezbradica, D.,& Nikačević, N.. (2022). Kinetic Model for Galacto-Oligosaccharide Synthesis. in Industrial and Engineering Chemistry Research
American Chemical Society., 61(38), 14189-14198.
https://doi.org/10.1021/acs.iecr.2c02053

Pravilović R, Todić B, Simović M, Banjanac K, Bezbradica D, Nikačević N. Kinetic Model for Galacto-Oligosaccharide Synthesis. in Industrial and Engineering Chemistry Research. 2022;61(38):14189-14198.
doi:10.1021/acs.iecr.2c02053 .

Pravilović, Radoslava, Todić, Branislav, Simović, Milica, Banjanac, Katarina, Bezbradica, Dejan, Nikačević, Nikola, "Kinetic Model for Galacto-Oligosaccharide Synthesis" in Industrial and Engineering Chemistry Research, 61, no. 38 (2022):14189-14198,
https://doi.org/10.1021/acs.iecr.2c02053 . .

TY  - CONF
AU  - Veljković, Milica
AU  - Petrov Ivanković, Anja
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Mitrušić, Ana
AU  - Katić, Katarina
AU  - Marinković, Aleksandar
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5998
AB  - Fructo-oligosaccharides (FOS) are recognized as prebiotic compounds which have the ability to stimulate the growth of gut microbiota, microorganisms that positively affect human health. Additionally, they have excellent nutrition- and health-relevant properties such as low caloric, non-cariogenicity, the ability to reduce total serum cholesterol levels, and many others. FOS can be found in various vegetables and fruits, but in low concentrations, which represents the main reason for their production on an industrial level. Production of FOS is predominantly based on the enzymatic transformation of sucrose using enzymes with transfructosylation activity such as fructosyltransferases or β-fructofuranosidases at the higher sucrose concentrations. In recent years, FOS growing market demand mainly as ingredients for food applications (bakery products, sweets, different types of beverages) has been increased, so there is a necessity for the development of new enzymatic systems for production of FOS with high yields and productivities. Enzyme immobilization techniques have been proposed as one of the potential solutions. Thus, this work aimed to evaluate the potential of amino-modified and cyanuric chloride functionalized amino-modified nonporous fumed silica nanoparticles (AFNS and CCAFNS, respectively) for the development of efficient nanobiocatalysts for application in the biosynthesis of FOS. Selected modified nanocarriers were applied for the immobilization of fructosyltransferase (FTase) from commercial enzyme preparation Pectinex® Ultra SP-L whereby the effects of immobilization parameters like initial enzyme concentration, immobilization time and pH were analysed. Among both used nanocarriers, the one with chloride groups (CCAFNS) exhibited the highest FTase binding capacity of 89 mg/g of support with the efficiency of 35 % at an initial enzyme concentration of 250 mg/g of support, pH 6.0, and immobilization time of 2.5 h. By examining the influence of immobilization time, it was found that the highest activity of 1576 IU/g of support was demonstrated by FTase immobilized on AFNS after 5 h, while FTase covalently immobilized on CCAFNS, exhibited activity of 1122 IU/g of support. According to the achieved activity, both obtained nanobiocatalysts were further applied in FOS production which was performed at 50 ⁰C and sucrose concentration of 500 g/l during 48 h. Apparently, with CCAFNS preparation FOS production of 14 g/l/h was achieved as compared to 5.9 g/l/h by AFNS preparation. Since in both cases, high sucrose conversion of 89 % was accomplished, it can be concluded that covalent immobilization of FTase on CCAFNS has the better catalytic capability for FOS production than FTase immobilized on AFNS via electrostatic interactions.
PB  - Faculty of Technology, University of East Sarajevo
C3  - VII International Congress “Engineering, Environment and Materials in Process Industry“
T1  - DEVELOPMENT OF FRUCTOSYLTRANSFERASE NANOBIOCATALYST FOR APPLICATION IN SYNTHESIS OF BIOACTIVE FRUCTO-OLIGOSACCHARIDES
SP  - ENG-32
DO  - 10.7251/EEMEN2101314V
ER  - 

@conference{
author = "Veljković, Milica and Petrov Ivanković, Anja and Simović, Milica and Banjanac, Katarina and Mitrušić, Ana and Katić, Katarina and Marinković, Aleksandar",
year = "2021",
abstract = "Fructo-oligosaccharides (FOS) are recognized as prebiotic compounds which have the ability to stimulate the growth of gut microbiota, microorganisms that positively affect human health. Additionally, they have excellent nutrition- and health-relevant properties such as low caloric, non-cariogenicity, the ability to reduce total serum cholesterol levels, and many others. FOS can be found in various vegetables and fruits, but in low concentrations, which represents the main reason for their production on an industrial level. Production of FOS is predominantly based on the enzymatic transformation of sucrose using enzymes with transfructosylation activity such as fructosyltransferases or β-fructofuranosidases at the higher sucrose concentrations. In recent years, FOS growing market demand mainly as ingredients for food applications (bakery products, sweets, different types of beverages) has been increased, so there is a necessity for the development of new enzymatic systems for production of FOS with high yields and productivities. Enzyme immobilization techniques have been proposed as one of the potential solutions. Thus, this work aimed to evaluate the potential of amino-modified and cyanuric chloride functionalized amino-modified nonporous fumed silica nanoparticles (AFNS and CCAFNS, respectively) for the development of efficient nanobiocatalysts for application in the biosynthesis of FOS. Selected modified nanocarriers were applied for the immobilization of fructosyltransferase (FTase) from commercial enzyme preparation Pectinex® Ultra SP-L whereby the effects of immobilization parameters like initial enzyme concentration, immobilization time and pH were analysed. Among both used nanocarriers, the one with chloride groups (CCAFNS) exhibited the highest FTase binding capacity of 89 mg/g of support with the efficiency of 35 % at an initial enzyme concentration of 250 mg/g of support, pH 6.0, and immobilization time of 2.5 h. By examining the influence of immobilization time, it was found that the highest activity of 1576 IU/g of support was demonstrated by FTase immobilized on AFNS after 5 h, while FTase covalently immobilized on CCAFNS, exhibited activity of 1122 IU/g of support. According to the achieved activity, both obtained nanobiocatalysts were further applied in FOS production which was performed at 50 ⁰C and sucrose concentration of 500 g/l during 48 h. Apparently, with CCAFNS preparation FOS production of 14 g/l/h was achieved as compared to 5.9 g/l/h by AFNS preparation. Since in both cases, high sucrose conversion of 89 % was accomplished, it can be concluded that covalent immobilization of FTase on CCAFNS has the better catalytic capability for FOS production than FTase immobilized on AFNS via electrostatic interactions.",
publisher = "Faculty of Technology, University of East Sarajevo",
journal = "VII International Congress “Engineering, Environment and Materials in Process Industry“",
title = "DEVELOPMENT OF FRUCTOSYLTRANSFERASE NANOBIOCATALYST FOR APPLICATION IN SYNTHESIS OF BIOACTIVE FRUCTO-OLIGOSACCHARIDES",
pages = "ENG-32",
doi = "10.7251/EEMEN2101314V"
}

Veljković, M., Petrov Ivanković, A., Simović, M., Banjanac, K., Mitrušić, A., Katić, K.,& Marinković, A.. (2021). DEVELOPMENT OF FRUCTOSYLTRANSFERASE NANOBIOCATALYST FOR APPLICATION IN SYNTHESIS OF BIOACTIVE FRUCTO-OLIGOSACCHARIDES. in VII International Congress “Engineering, Environment and Materials in Process Industry“
Faculty of Technology, University of East Sarajevo., ENG-32.
https://doi.org/10.7251/EEMEN2101314V

Veljković M, Petrov Ivanković A, Simović M, Banjanac K, Mitrušić A, Katić K, Marinković A. DEVELOPMENT OF FRUCTOSYLTRANSFERASE NANOBIOCATALYST FOR APPLICATION IN SYNTHESIS OF BIOACTIVE FRUCTO-OLIGOSACCHARIDES. in VII International Congress “Engineering, Environment and Materials in Process Industry“. 2021;:ENG-32.
doi:10.7251/EEMEN2101314V .

Veljković, Milica, Petrov Ivanković, Anja, Simović, Milica, Banjanac, Katarina, Mitrušić, Ana, Katić, Katarina, Marinković, Aleksandar, "DEVELOPMENT OF FRUCTOSYLTRANSFERASE NANOBIOCATALYST FOR APPLICATION IN SYNTHESIS OF BIOACTIVE FRUCTO-OLIGOSACCHARIDES" in VII International Congress “Engineering, Environment and Materials in Process Industry“ (2021):ENG-32,
https://doi.org/10.7251/EEMEN2101314V . .

TY  - JOUR
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Đukić-Vuković, Aleksandra
AU  - Ilic, Mila, V
AU  - Simović, Milica
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4791
AB  - Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391 +/- 0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.
T2  - Hemijska industrija
T1  - Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology
EP  - 146
IS  - 3
SP  - 135
VL  - 75
DO  - 10.2298/HEMIND200710015M
ER  - 

@article{
author = "Miljković, Miona and Davidović, Slađana and Đukić-Vuković, Aleksandra and Ilic, Mila, V and Simović, Milica and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391 +/- 0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.",
journal = "Hemijska industrija",
title = "Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology",
pages = "146-135",
number = "3",
volume = "75",
doi = "10.2298/HEMIND200710015M"
}

Miljković, M., Davidović, S., Đukić-Vuković, A., Ilic, M. V., Simović, M., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology. in Hemijska industrija, 75(3), 135-146.
https://doi.org/10.2298/HEMIND200710015M

Miljković M, Davidović S, Đukić-Vuković A, Ilic MV, Simović M, Rajilić-Stojanović M, Dimitrijević-Branković S. Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology. in Hemijska industrija. 2021;75(3):135-146.
doi:10.2298/HEMIND200710015M .

Miljković, Miona, Davidović, Slađana, Đukić-Vuković, Aleksandra, Ilic, Mila, V, Simović, Milica, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology" in Hemijska industrija, 75, no. 3 (2021):135-146,
https://doi.org/10.2298/HEMIND200710015M . .

TY  - JOUR
AU  - Katić, Katarina
AU  - Banjanac, Katarina
AU  - Simović, Milica
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Marinković, Aleksandar
AU  - Bezbradica, Dejan
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4864
AB  - In this study, the suitability of fumed silica nanoparticles (FNS) and its derivatives (amino-modified FNS (AFNS), cyanuric chloride-activated AFNS (CCAFNS) and epoxy-modified FNS (GFNS)), for covalent immobilisation of two commercial protease preparations Alcalase(R) and Flavourzyme(R) was investigated. The highest hydrolytic activities of immobilised preparations were 25 IU g(-1) support (Alcalase-GFNS) and 2.95 IU g(-1) support (Flavourzyme-CCAFNS). Furthermore, the immobilised preparations showed 43% and 20% of initial specific activities of commercial protease preparations, respectively. Flavourzyme-CCAFNS also exhibited the highest exopeptidase activity of 22.83 L-pNAU g(-1) support. Finally, these two nanobiocatalysts were successfully applied for hydrolysis of sunflower meal protein isolate (SMPI), providing two times higher hydrolysis yields in comparison to free enzymes, justifying the applied immobilisation process. Namely, the highest hydrolysis yield (30%) was gained by the sequential hydrolysis with Alcalase-GFNS and Flavourzyme-CCAFNS, which resulted in the formation of small hydrophobic and hydrophilic peptides,   lt = 5 kDa, confirmed by HPLC analysis and electrophoretic separation.
PB  - John Wiley and Sons Inc.
T2  - International Journal of Food Science and Technology
T1  - Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate
EP  - 4297
IS  - 9
SP  - 4287
VL  - 56
DO  - 10.1111/ijfs.15189
ER  - 

@article{
author = "Katić, Katarina and Banjanac, Katarina and Simović, Milica and Ćorović, Marija and Milivojević, Ana and Marinković, Aleksandar and Bezbradica, Dejan",
year = "2021",
abstract = "In this study, the suitability of fumed silica nanoparticles (FNS) and its derivatives (amino-modified FNS (AFNS), cyanuric chloride-activated AFNS (CCAFNS) and epoxy-modified FNS (GFNS)), for covalent immobilisation of two commercial protease preparations Alcalase(R) and Flavourzyme(R) was investigated. The highest hydrolytic activities of immobilised preparations were 25 IU g(-1) support (Alcalase-GFNS) and 2.95 IU g(-1) support (Flavourzyme-CCAFNS). Furthermore, the immobilised preparations showed 43% and 20% of initial specific activities of commercial protease preparations, respectively. Flavourzyme-CCAFNS also exhibited the highest exopeptidase activity of 22.83 L-pNAU g(-1) support. Finally, these two nanobiocatalysts were successfully applied for hydrolysis of sunflower meal protein isolate (SMPI), providing two times higher hydrolysis yields in comparison to free enzymes, justifying the applied immobilisation process. Namely, the highest hydrolysis yield (30%) was gained by the sequential hydrolysis with Alcalase-GFNS and Flavourzyme-CCAFNS, which resulted in the formation of small hydrophobic and hydrophilic peptides,   lt = 5 kDa, confirmed by HPLC analysis and electrophoretic separation.",
publisher = "John Wiley and Sons Inc.",
journal = "International Journal of Food Science and Technology",
title = "Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate",
pages = "4297-4287",
number = "9",
volume = "56",
doi = "10.1111/ijfs.15189"
}

Katić, K., Banjanac, K., Simović, M., Ćorović, M., Milivojević, A., Marinković, A.,& Bezbradica, D.. (2021). Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate. in International Journal of Food Science and Technology
John Wiley and Sons Inc.., 56(9), 4287-4297.
https://doi.org/10.1111/ijfs.15189

Katić K, Banjanac K, Simović M, Ćorović M, Milivojević A, Marinković A, Bezbradica D. Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate. in International Journal of Food Science and Technology. 2021;56(9):4287-4297.
doi:10.1111/ijfs.15189 .

Katić, Katarina, Banjanac, Katarina, Simović, Milica, Ćorović, Marija, Milivojević, Ana, Marinković, Aleksandar, Bezbradica, Dejan, "Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate" in International Journal of Food Science and Technology, 56, no. 9 (2021):4287-4297,
https://doi.org/10.1111/ijfs.15189 . .

TY  - JOUR
AU  - Veljković, Milica B.
AU  - Modi, Ankita
AU  - Petrov, Anja I.
AU  - Ćorović, Marija M.
AU  - Milivojević, Ana D.
AU  - Banjanac, Katarina M.
AU  - Simović, Milica B.
AU  - Bezbradica, Dejan I.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5999
AB  - The obvious benefits of employing prebiotics as functional components in many foods
and feed products have resulted in higher demand for their industrial production, necessitating the
development of more efficient and cost-effective manufacturing procedures. As a result, the goal of
this study was to synthesize confirmed prebiotics, namely fructo-oligosaccharides (FOS), using
sucrose as a substrate, since it allows the synthesis of oligosaccharides with lower polymerization
degree, and consequently, a more pronounced prebiotic effect. Due to its availability, low market
price, and high stability under industrial conditions, a commercial enzymatic mixture, Pectinex®
Ultra SP-L, is used as a source of enzyme – fructosyltransferase (FTase). By varying key
experimental conditions such as pH, temperature, enzyme and substrate concentrations, as well as
the duration of the process, the composition of the FOS mixture can be adjusted to fit the potential
applications. It was found that by performing the reaction in an aqueous medium (pH 7), at a
temperature of 50 °C using an enzyme concentration of 1% (v/v) and any sucrose concentration in
the range of 200-700 g/L, it was possible to achieve maximum FOS yield of 60% of total
carbohydrates within a 24 h. The produced syrup with a high content of FOS can be further used as an adequate food additive, or else, developed processes should be used for the transformation of various food products (such as juices, jams, fillings, candies, cakes, etc.) in which sucrose
dominates, creating products with lower caloric and higher functional value
AB  - Razvijanje svesti o brojnim prednostima upotrebe prebiotika kao funkcionalnih
sastojaka prehrambenih proizvoda i hrane za životinje rezultira povećanjem potražnje, a
samim tim i potrebom za povećanjem proizvodnje prebiotika na industrijskom novou.
Ovo dodatno zahteva i razvijanje efikasnijih i ekonomičnijih procesa proizvodnje
prebiotika. Cilj ovog rada bila je sinteza potvrđenih prebiotika - frukto-oligosaharida
(FOS), transformacijom saharoze. Ova reakcija je odabrana prvenstveno sa aspekta
mogućnosti sinteze oligosaharida manjih molarnih masa sa izraženijim prebiotskim
efektom koji se ovim putem dobijaju. Kao izvor enzima korišćena je komercijalna
enzimska smeša Pectinex® Ultra SP-L, čijom se upotrebom zbog niske cene na tržištu,
dodatno utiče na ekonomičnost procesa. Ispitivanjem uticaja reakcionih uslova poput pH,
temperature, koncentracije enzima i supstrata utvrđeno je da se variranjem uslova, kao i
dužine trajanja procesa može značajno prilagoditi sastav dobijene smeše FOS. Stoga,
utvrđeno je da se izvođenjem reakcije u vodenoj sredini (pH 7), na temperaturi od 50 °C
upotrebom konentracije enzima od 1% (v/v) i koncentracije saharoze od 500 g/L može
ostvariti maksimalni prinos FOS od 60% u ukupnim šećerima. Ovako dobijen sirup sa
visokim udelom FOS se može koristiti kao aditiv proizvodima, dok se sa druge strane
razvijeni proces može iskoristiti za direktnu transformaciju prehrambenih proizvoda
(poput sokova, džema, nadeva, bombona, kolača i dr.) u kojima dominira saharoza kao
osnovni izvor šećera, stvarajući na taj način proizvode sa manjom kalorijskom, a većom
funkcionalnom vrednošću.
PB  - Institute for Food Technology
T2  - Food and Feed Research
T1  - Enzymatic synthesis of fructo-oligosaccharides using Pectinex® Ultra SP-L: a study of experimental conditions
T1  - Enzimska sinteza frukto-oligosaharida upotrebom Pectinex® Ultra SP-L: ispitivanje uticaja eksperimentalnih uslova
EP  - 211
IS  - 2
SP  - 201
VL  - 48
DO  - 10.5937/ffr0-34517
ER  - 

@article{
author = "Veljković, Milica B. and Modi, Ankita and Petrov, Anja I. and Ćorović, Marija M. and Milivojević, Ana D. and Banjanac, Katarina M. and Simović, Milica B. and Bezbradica, Dejan I.",
year = "2021",
abstract = "The obvious benefits of employing prebiotics as functional components in many foods
and feed products have resulted in higher demand for their industrial production, necessitating the
development of more efficient and cost-effective manufacturing procedures. As a result, the goal of
this study was to synthesize confirmed prebiotics, namely fructo-oligosaccharides (FOS), using
sucrose as a substrate, since it allows the synthesis of oligosaccharides with lower polymerization
degree, and consequently, a more pronounced prebiotic effect. Due to its availability, low market
price, and high stability under industrial conditions, a commercial enzymatic mixture, Pectinex®
Ultra SP-L, is used as a source of enzyme – fructosyltransferase (FTase). By varying key
experimental conditions such as pH, temperature, enzyme and substrate concentrations, as well as
the duration of the process, the composition of the FOS mixture can be adjusted to fit the potential
applications. It was found that by performing the reaction in an aqueous medium (pH 7), at a
temperature of 50 °C using an enzyme concentration of 1% (v/v) and any sucrose concentration in
the range of 200-700 g/L, it was possible to achieve maximum FOS yield of 60% of total
carbohydrates within a 24 h. The produced syrup with a high content of FOS can be further used as an adequate food additive, or else, developed processes should be used for the transformation of various food products (such as juices, jams, fillings, candies, cakes, etc.) in which sucrose
dominates, creating products with lower caloric and higher functional value, Razvijanje svesti o brojnim prednostima upotrebe prebiotika kao funkcionalnih
sastojaka prehrambenih proizvoda i hrane za životinje rezultira povećanjem potražnje, a
samim tim i potrebom za povećanjem proizvodnje prebiotika na industrijskom novou.
Ovo dodatno zahteva i razvijanje efikasnijih i ekonomičnijih procesa proizvodnje
prebiotika. Cilj ovog rada bila je sinteza potvrđenih prebiotika - frukto-oligosaharida
(FOS), transformacijom saharoze. Ova reakcija je odabrana prvenstveno sa aspekta
mogućnosti sinteze oligosaharida manjih molarnih masa sa izraženijim prebiotskim
efektom koji se ovim putem dobijaju. Kao izvor enzima korišćena je komercijalna
enzimska smeša Pectinex® Ultra SP-L, čijom se upotrebom zbog niske cene na tržištu,
dodatno utiče na ekonomičnost procesa. Ispitivanjem uticaja reakcionih uslova poput pH,
temperature, koncentracije enzima i supstrata utvrđeno je da se variranjem uslova, kao i
dužine trajanja procesa može značajno prilagoditi sastav dobijene smeše FOS. Stoga,
utvrđeno je da se izvođenjem reakcije u vodenoj sredini (pH 7), na temperaturi od 50 °C
upotrebom konentracije enzima od 1% (v/v) i koncentracije saharoze od 500 g/L može
ostvariti maksimalni prinos FOS od 60% u ukupnim šećerima. Ovako dobijen sirup sa
visokim udelom FOS se može koristiti kao aditiv proizvodima, dok se sa druge strane
razvijeni proces može iskoristiti za direktnu transformaciju prehrambenih proizvoda
(poput sokova, džema, nadeva, bombona, kolača i dr.) u kojima dominira saharoza kao
osnovni izvor šećera, stvarajući na taj način proizvode sa manjom kalorijskom, a većom
funkcionalnom vrednošću.",
publisher = "Institute for Food Technology",
journal = "Food and Feed Research",
title = "Enzymatic synthesis of fructo-oligosaccharides using Pectinex® Ultra SP-L: a study of experimental conditions, Enzimska sinteza frukto-oligosaharida upotrebom Pectinex® Ultra SP-L: ispitivanje uticaja eksperimentalnih uslova",
pages = "211-201",
number = "2",
volume = "48",
doi = "10.5937/ffr0-34517"
}

Veljković, M. B., Modi, A., Petrov, A. I., Ćorović, M. M., Milivojević, A. D., Banjanac, K. M., Simović, M. B.,& Bezbradica, D. I.. (2021). Enzymatic synthesis of fructo-oligosaccharides using Pectinex® Ultra SP-L: a study of experimental conditions. in Food and Feed Research
Institute for Food Technology., 48(2), 201-211.
https://doi.org/10.5937/ffr0-34517

Veljković MB, Modi A, Petrov AI, Ćorović MM, Milivojević AD, Banjanac KM, Simović MB, Bezbradica DI. Enzymatic synthesis of fructo-oligosaccharides using Pectinex® Ultra SP-L: a study of experimental conditions. in Food and Feed Research. 2021;48(2):201-211.
doi:10.5937/ffr0-34517 .

Veljković, Milica B., Modi, Ankita, Petrov, Anja I., Ćorović, Marija M., Milivojević, Ana D., Banjanac, Katarina M., Simović, Milica B., Bezbradica, Dejan I., "Enzymatic synthesis of fructo-oligosaccharides using Pectinex® Ultra SP-L: a study of experimental conditions" in Food and Feed Research, 48, no. 2 (2021):201-211,
https://doi.org/10.5937/ffr0-34517 . .