TY  - JOUR
AU  - Milovanović, Stoja
AU  - Marković, Darka
AU  - Janković-Častvan, Ivona
AU  - Lukić, Ivana
PY  - 2024-05
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7258
AB  - This study focuses on the development of bioactive materials using environmentally friendly techniques, renewable, biocompatible, and biodegradable polysaccharide, as well as natural bioactive compounds (NBCs) found in plant extracts. First, cornstarch aerogels with a porosity of 86 % and a specific surface area of 225 m2/g were produced via supercritical CO2- assisted drying. Further, thymol, citronellol, carvacrol, and eugenol were incorporated into the aerogels by supercritical CO2- assisted impregnation, which allowed variation in loadings of NBCs (12.8–17.6 %). Interaction between cornstarch aerogels and NBCs determined impregnation rate, pore wall thickness (in the range 18–95 nm), liquid absorption capacity (from 265 to 569 %), dehydration mass loss, and release in phosphate-buffered saline. Controlled release of NBCs was maintained over a 3-day period. Moreover, impregnated aerogels showed a significant antioxidant effect with the highest value for DPPH radical inhibition of 25.5 % determined for the aerogels impregnated with eugenol. Notable antimicrobial activity against tested Gram-negative bacteria, Gram-positive bacteria, and fungi was also observed, being the highest for thymol-loaded aerogel with the diameter of the inhibition zones of up to 37.5 mm. This work shows a promising green approach for the production of bioactive two-component starch-based materials for potential applications in skin infection treatment.
PB  - Elsevier Ltd.
T2  - Carbohydrate Polymers
T1  - Cornstarch aerogels with thymol, citronellol, carvacrol, and eugenol prepared by supercritical CO2- assisted techniques for potential biomedical applications
SP  - 121874
VL  - 331
DO  - 10.1016/j.carbpol.2024.121874
ER  - 

@article{
author = "Milovanović, Stoja and Marković, Darka and Janković-Častvan, Ivona and Lukić, Ivana",
year = "2024-05",
abstract = "This study focuses on the development of bioactive materials using environmentally friendly techniques, renewable, biocompatible, and biodegradable polysaccharide, as well as natural bioactive compounds (NBCs) found in plant extracts. First, cornstarch aerogels with a porosity of 86 % and a specific surface area of 225 m2/g were produced via supercritical CO2- assisted drying. Further, thymol, citronellol, carvacrol, and eugenol were incorporated into the aerogels by supercritical CO2- assisted impregnation, which allowed variation in loadings of NBCs (12.8–17.6 %). Interaction between cornstarch aerogels and NBCs determined impregnation rate, pore wall thickness (in the range 18–95 nm), liquid absorption capacity (from 265 to 569 %), dehydration mass loss, and release in phosphate-buffered saline. Controlled release of NBCs was maintained over a 3-day period. Moreover, impregnated aerogels showed a significant antioxidant effect with the highest value for DPPH radical inhibition of 25.5 % determined for the aerogels impregnated with eugenol. Notable antimicrobial activity against tested Gram-negative bacteria, Gram-positive bacteria, and fungi was also observed, being the highest for thymol-loaded aerogel with the diameter of the inhibition zones of up to 37.5 mm. This work shows a promising green approach for the production of bioactive two-component starch-based materials for potential applications in skin infection treatment.",
publisher = "Elsevier Ltd.",
journal = "Carbohydrate Polymers",
title = "Cornstarch aerogels with thymol, citronellol, carvacrol, and eugenol prepared by supercritical CO2- assisted techniques for potential biomedical applications",
pages = "121874",
volume = "331",
doi = "10.1016/j.carbpol.2024.121874"
}

Milovanović, S., Marković, D., Janković-Častvan, I.,& Lukić, I.. (2024-05). Cornstarch aerogels with thymol, citronellol, carvacrol, and eugenol prepared by supercritical CO2- assisted techniques for potential biomedical applications. in Carbohydrate Polymers
Elsevier Ltd.., 331, 121874.
https://doi.org/10.1016/j.carbpol.2024.121874

Milovanović S, Marković D, Janković-Častvan I, Lukić I. Cornstarch aerogels with thymol, citronellol, carvacrol, and eugenol prepared by supercritical CO2- assisted techniques for potential biomedical applications. in Carbohydrate Polymers. 2024;331:121874.
doi:10.1016/j.carbpol.2024.121874 .

Milovanović, Stoja, Marković, Darka, Janković-Častvan, Ivona, Lukić, Ivana, "Cornstarch aerogels with thymol, citronellol, carvacrol, and eugenol prepared by supercritical CO2- assisted techniques for potential biomedical applications" in Carbohydrate Polymers, 331 (2024-05):121874,
https://doi.org/10.1016/j.carbpol.2024.121874 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Lukić, Ivana
AU  - Krgović, Nemanja
AU  - Tadić, Vanja
AU  - Radovanović, Željko
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2024-04
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7140
AB  - The study aimed toward the development of biocompatible and biodegradable polymeric films (starch-chitosan and starch-chitosan-cyclodextrin) with biologically valuable green tea leaf extract using an environmentally friendly integrated process of supercritical fluid extraction (SFE) and supercritical solvent impregnation (SSI). The SFE-SSI process parameters were selected through the initial estimation of the SFE parameters effect (temperatures 35 and 70 °C and the addition of co-solvents ethanol, ethanol/water, and water). The present early-stage work showed that an increase in temperature increased extraction yield (from 0.9 to 4.2%) and caffeine content while it decreased polyphenolic and pigment content as well as antioxidant activity and extract loading onto films (from 7.1 to 0.1%). The addition of co-solvents to sc-CO2 had an adverse effect on extract yield, composition, and loading. The highest recorded total phenolic, flavonoid, chlorophyll A, and caffeine content in extracts were 136.5 mg GE/g, 22.7 mg RE/g, 8.1 mg/g, and 460.0 mg/g, respectively.
PB  - Elsevier B.V.
T2  - Journal of Supercritical Fluids
T1  - Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films
SP  - 106163
VL  - 206
DO  - 10.1016/j.supflu.2023.106163
ER  - 

@article{
author = "Milovanović, Stoja and Lukić, Ivana and Krgović, Nemanja and Tadić, Vanja and Radovanović, Željko and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2024-04",
abstract = "The study aimed toward the development of biocompatible and biodegradable polymeric films (starch-chitosan and starch-chitosan-cyclodextrin) with biologically valuable green tea leaf extract using an environmentally friendly integrated process of supercritical fluid extraction (SFE) and supercritical solvent impregnation (SSI). The SFE-SSI process parameters were selected through the initial estimation of the SFE parameters effect (temperatures 35 and 70 °C and the addition of co-solvents ethanol, ethanol/water, and water). The present early-stage work showed that an increase in temperature increased extraction yield (from 0.9 to 4.2%) and caffeine content while it decreased polyphenolic and pigment content as well as antioxidant activity and extract loading onto films (from 7.1 to 0.1%). The addition of co-solvents to sc-CO2 had an adverse effect on extract yield, composition, and loading. The highest recorded total phenolic, flavonoid, chlorophyll A, and caffeine content in extracts were 136.5 mg GE/g, 22.7 mg RE/g, 8.1 mg/g, and 460.0 mg/g, respectively.",
publisher = "Elsevier B.V.",
journal = "Journal of Supercritical Fluids",
title = "Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films",
pages = "106163",
volume = "206",
doi = "10.1016/j.supflu.2023.106163"
}

Milovanović, S., Lukić, I., Krgović, N., Tadić, V., Radovanović, Ž., Tyśkiewicz, K.,& Konkol, M.. (2024-04). Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films. in Journal of Supercritical Fluids
Elsevier B.V.., 206, 106163.
https://doi.org/10.1016/j.supflu.2023.106163

Milovanović S, Lukić I, Krgović N, Tadić V, Radovanović Ž, Tyśkiewicz K, Konkol M. Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films. in Journal of Supercritical Fluids. 2024;206:106163.
doi:10.1016/j.supflu.2023.106163 .

Milovanović, Stoja, Lukić, Ivana, Krgović, Nemanja, Tadić, Vanja, Radovanović, Željko, Tyśkiewicz, Katarzyna, Konkol, Marcin, "Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films" in Journal of Supercritical Fluids, 206 (2024-04):106163,
https://doi.org/10.1016/j.supflu.2023.106163 . .

TY  - CONF
AU  - Kalagasidis Krušić, Melina
AU  - Lučić Škorić, Marija
AU  - Milovanović, Stoja
AU  - Panić, Vesna
AU  - Marković, Maja
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7392
AB  - The Polymer group of the Faculty of Technology and Metallurgy University of Belgrade deals with environment-sensitive hydrogel as carriersfor biomedical applications, including drug delivery, wound healing, and regenerative medicine. The first hydrogel generation synthesized in our labs was discs made of polyacrylamide and its derivatives. The synthesis was at higher temperatures (50 °C), with traditional crosslinker, N, N’-methylenebisacrylamide, and persulphate/pyrosulphate as initiator. Due to the growing demands for environmental sustainability, the general approach changed to reduce petrochemical raw materials and prepare eco-friendly materials focusing on 1) renewable polymers, initiators, and crosslinkers and 2) the application of simple, cost-effective, and eco-friendly approaches in hydrogel synthesis. To obtain the second hydrogel generation, we use renewable polymers: polysaccharides, proteins, and polyhydroxyalkanoates [1]. Still, the mechanical strength was weak. Hence, the applicationof carboxylic acids, methacrylic and itaconic, and N-isopropyl acrylamide improves mechanical properties and enhances the environmental stimuli of the carriers. Instead of traditional crosslinkers -organic molecules, we gave the advantage to plant extracts like genipin, sodium tripolyphosphate, citric acid, and calcium chloride. We made beads, microgels, aerogels, discs, films, and cylinders sensitive to pH, temperature, magnetic field, or specific molecules such as glucose. Facing an everyday challenge, improving drug delivery routes, especially for poorly water-soluble drugs, and finding an alternative to traditional antibiotics, encapsulation, and controlled release remain a challenge. Therefore, we used a mild condition (e.g., deep coating) to encapsulate/release traditional water-soluble and poorly water-soluble drugs, proteins, phenolic compounds, or supercritical carbon dioxide (scCO2) for thymol, carvacrol, and eugenol, a promising alternative for traditional antibiotics [2]. Furthermore, we reduced the hydrogel production temperature to ambient conditions and made a simple and cost-effective production process that doesn't require special equipment. The promising results we got pushed us further. So, the current research focuses on the encapsulation/controlled release of antioxidant phenolic compounds extracted from orange peel waste by applying ultrasonic-assisted extraction and deep eutectic solvent(DES) based on glycerol: urea: water. We use a new initiator system based on vitamin C and H2O2to polymerizea methacrylic acid[3]that could be useful for other monomers.As a concluding remark, we achieved significant progress in hydrogels encouragingus to continue further.
PB  - Savez hemijskih inženjera Srbije
C3  - Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts
T1  - Environmentally friendly hydrogels for medical and pharmaceutical applications
IS  - 1S
SP  - 27
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7392
ER  - 

@conference{
author = "Kalagasidis Krušić, Melina and Lučić Škorić, Marija and Milovanović, Stoja and Panić, Vesna and Marković, Maja",
year = "2024",
abstract = "The Polymer group of the Faculty of Technology and Metallurgy University of Belgrade deals with environment-sensitive hydrogel as carriersfor biomedical applications, including drug delivery, wound healing, and regenerative medicine. The first hydrogel generation synthesized in our labs was discs made of polyacrylamide and its derivatives. The synthesis was at higher temperatures (50 °C), with traditional crosslinker, N, N’-methylenebisacrylamide, and persulphate/pyrosulphate as initiator. Due to the growing demands for environmental sustainability, the general approach changed to reduce petrochemical raw materials and prepare eco-friendly materials focusing on 1) renewable polymers, initiators, and crosslinkers and 2) the application of simple, cost-effective, and eco-friendly approaches in hydrogel synthesis. To obtain the second hydrogel generation, we use renewable polymers: polysaccharides, proteins, and polyhydroxyalkanoates [1]. Still, the mechanical strength was weak. Hence, the applicationof carboxylic acids, methacrylic and itaconic, and N-isopropyl acrylamide improves mechanical properties and enhances the environmental stimuli of the carriers. Instead of traditional crosslinkers -organic molecules, we gave the advantage to plant extracts like genipin, sodium tripolyphosphate, citric acid, and calcium chloride. We made beads, microgels, aerogels, discs, films, and cylinders sensitive to pH, temperature, magnetic field, or specific molecules such as glucose. Facing an everyday challenge, improving drug delivery routes, especially for poorly water-soluble drugs, and finding an alternative to traditional antibiotics, encapsulation, and controlled release remain a challenge. Therefore, we used a mild condition (e.g., deep coating) to encapsulate/release traditional water-soluble and poorly water-soluble drugs, proteins, phenolic compounds, or supercritical carbon dioxide (scCO2) for thymol, carvacrol, and eugenol, a promising alternative for traditional antibiotics [2]. Furthermore, we reduced the hydrogel production temperature to ambient conditions and made a simple and cost-effective production process that doesn't require special equipment. The promising results we got pushed us further. So, the current research focuses on the encapsulation/controlled release of antioxidant phenolic compounds extracted from orange peel waste by applying ultrasonic-assisted extraction and deep eutectic solvent(DES) based on glycerol: urea: water. We use a new initiator system based on vitamin C and H2O2to polymerizea methacrylic acid[3]that could be useful for other monomers.As a concluding remark, we achieved significant progress in hydrogels encouragingus to continue further.",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts",
title = "Environmentally friendly hydrogels for medical and pharmaceutical applications",
number = "1S",
pages = "27",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7392"
}

Kalagasidis Krušić, M., Lučić Škorić, M., Milovanović, S., Panić, V.,& Marković, M.. (2024). Environmentally friendly hydrogels for medical and pharmaceutical applications. in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts
Savez hemijskih inženjera Srbije., 78(1S), 27.
https://hdl.handle.net/21.15107/rcub_technorep_7392

Kalagasidis Krušić M, Lučić Škorić M, Milovanović S, Panić V, Marković M. Environmentally friendly hydrogels for medical and pharmaceutical applications. in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts. 2024;78(1S):27.
https://hdl.handle.net/21.15107/rcub_technorep_7392 .

Kalagasidis Krušić, Melina, Lučić Škorić, Marija, Milovanović, Stoja, Panić, Vesna, Marković, Maja, "Environmentally friendly hydrogels for medical and pharmaceutical applications" in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts, 78, no. 1S (2024):27,
https://hdl.handle.net/21.15107/rcub_technorep_7392 .

TY  - CONF
AU  - Lukić, Ivana
AU  - Milovanović, Stoja
AU  - Krgović, Nemanja
AU  - Tadić, Vanja
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6907
AB  - Green tea is considered to be a plant with a variety of health benefits due to its antioxidative, antimicrobial,
anti-inflammatory, and anticancer activity. Besides the most important bioactive constituents, including
polyphenols, especially catechins, and flavonoids, green tea is rich in caffeine, and such complex composition
makes its extract suitable as a component of active food packaging or skin care systems [1,2]. The
technologies commonly proposed to obtain such materials involve some limitations, mainly related to the use
of liquid organic solvents that lead to low penetration of the active compound into the polymeric platform and,
consequently, limited loading efficiencies, non-unique dispersion, and solvent residue in the final material. An
excellent technique for direct loading of natural bioactive compounds from plant materials into polymeric
carriers that can overcome the aforementioned challenges is an integrated process accomplished by merging
supercritical fluid extraction (SFE) and supercritical solvent impregnation (SSI). Therefore, in this study, the
preparation of two types of polymer films and their functionalization with green tea leaf extract using the SFESSI
process was tested.
Starch/chitosan (SC) films were prepared by mixing the 1% chitosan solution with the 1% starch solution in a
mass ratio of 1:1, while starch/chitosan/β-cyclodextrin (SCCD) films were prepared by addition of 1% β-
cyclodextrin to starch and chitosan to obtain the ratio of 1:1:1. After solvent casting and drying at room
temperature, the films were additionally dried in an oven overnight at 35 °C. Initially, supercritical CO2
(scCO2) extraction was investigated as a method for isolating the extract from green tea at temperatures of 35
and 70 °C and pressure of 30 MPa, with and without the ethanol as a co-solvent. The obtained extraction yields
ranged from 0.91 to 4.22%, being the highest at a higher temperature. The chemical composition of the
obtained extracts was determined, as well as the total phenolic content (TPC), total flavonoid content (TFC),
total chlorophyll, and total carotenoid content. HPLC analysis revealed that caffeine was the major constituent
of all extracts with the amount ranging from 24.1 to 202.9 mg/g. The IC50 values determined using the DPPH
assay showed that the extract obtained at 35 °C with the addition of ethanol exhibited the strongest antioxidant
activity. After determining the SFE conditions that gave the green tea extract with high content of bioactive
compounds, prepared films were impregnated using the integrated SFE-SSI process for 4 h at the same
conditions as scCO2 extraction. The loading of green tea extracts onto films was up to 7.7% showing that lower
temperature (35 °C) and the addition of ethanol had a favourable influence. The presence of the extract on the
surface of impregnated samples was confirmed by FTIR analysis.
PB  - Budapest : University of Technology and Economics
C3  - Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
T1  - Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films
SP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6907
ER  - 

@conference{
author = "Lukić, Ivana and Milovanović, Stoja and Krgović, Nemanja and Tadić, Vanja and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2023",
abstract = "Green tea is considered to be a plant with a variety of health benefits due to its antioxidative, antimicrobial,
anti-inflammatory, and anticancer activity. Besides the most important bioactive constituents, including
polyphenols, especially catechins, and flavonoids, green tea is rich in caffeine, and such complex composition
makes its extract suitable as a component of active food packaging or skin care systems [1,2]. The
technologies commonly proposed to obtain such materials involve some limitations, mainly related to the use
of liquid organic solvents that lead to low penetration of the active compound into the polymeric platform and,
consequently, limited loading efficiencies, non-unique dispersion, and solvent residue in the final material. An
excellent technique for direct loading of natural bioactive compounds from plant materials into polymeric
carriers that can overcome the aforementioned challenges is an integrated process accomplished by merging
supercritical fluid extraction (SFE) and supercritical solvent impregnation (SSI). Therefore, in this study, the
preparation of two types of polymer films and their functionalization with green tea leaf extract using the SFESSI
process was tested.
Starch/chitosan (SC) films were prepared by mixing the 1% chitosan solution with the 1% starch solution in a
mass ratio of 1:1, while starch/chitosan/β-cyclodextrin (SCCD) films were prepared by addition of 1% β-
cyclodextrin to starch and chitosan to obtain the ratio of 1:1:1. After solvent casting and drying at room
temperature, the films were additionally dried in an oven overnight at 35 °C. Initially, supercritical CO2
(scCO2) extraction was investigated as a method for isolating the extract from green tea at temperatures of 35
and 70 °C and pressure of 30 MPa, with and without the ethanol as a co-solvent. The obtained extraction yields
ranged from 0.91 to 4.22%, being the highest at a higher temperature. The chemical composition of the
obtained extracts was determined, as well as the total phenolic content (TPC), total flavonoid content (TFC),
total chlorophyll, and total carotenoid content. HPLC analysis revealed that caffeine was the major constituent
of all extracts with the amount ranging from 24.1 to 202.9 mg/g. The IC50 values determined using the DPPH
assay showed that the extract obtained at 35 °C with the addition of ethanol exhibited the strongest antioxidant
activity. After determining the SFE conditions that gave the green tea extract with high content of bioactive
compounds, prepared films were impregnated using the integrated SFE-SSI process for 4 h at the same
conditions as scCO2 extraction. The loading of green tea extracts onto films was up to 7.7% showing that lower
temperature (35 °C) and the addition of ethanol had a favourable influence. The presence of the extract on the
surface of impregnated samples was confirmed by FTIR analysis.",
publisher = "Budapest : University of Technology and Economics",
journal = "Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023",
title = "Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films",
pages = "124",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6907"
}

Lukić, I., Milovanović, S., Krgović, N., Tadić, V., Tyśkiewicz, K.,& Konkol, M.. (2023). Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
Budapest : University of Technology and Economics., 124.
https://hdl.handle.net/21.15107/rcub_technorep_6907

Lukić I, Milovanović S, Krgović N, Tadić V, Tyśkiewicz K, Konkol M. Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023. 2023;:124.
https://hdl.handle.net/21.15107/rcub_technorep_6907 .

Lukić, Ivana, Milovanović, Stoja, Krgović, Nemanja, Tadić, Vanja, Tyśkiewicz, Katarzyna, Konkol, Marcin, "Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films" in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 (2023):124,
https://hdl.handle.net/21.15107/rcub_technorep_6907 .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Grzegorczyk, Agnieszka
AU  - Świątek, Łukasz
AU  - Boguszewska, Anastazja
AU  - Kowalski, Rafał
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6713
AB  - Dandelion is a sustainable biomass that has occasional use in the food and pharmaceutical industry. However, commercial use of its extracts is restricted by the lack of systematic information on safety, composition, and biological activity. To broaden dandelion use, the present study brings new knowledge on extracts from dandelion seeds that could find application as safe and functional food ingredients. The employed two-step supercritical fluid extraction process, followed by the conventional extraction, enabled the separation of extracts with increased content of phenolics, tocopherols, and essential fatty acids. Those compounds were found to be the major components of dandelion extracts, reaching high values up to 799 mg GE/g, 249 mg α-TE/g, and 75%, respectively. The extracts also contained significant amounts of flavonoids (up to 20.5 mg QE/g) and pigments (chlorophyll A up to 1.0 g/kg, chlorophyll B up to 0.3 g/kg, and carotenoids up to 0.1 g/kg). This study revealed new bioproducts that express strong antioxidant (reaching IC50 values up to 132.5 μg/mL) and antimicrobial activity (with minimum inhibitory concentrations up to 80.0 μg/mL). Among the tested 23 microbial strains, the extracts exhibited the strongest activity against food-borne pathogens Micrococcus luteus and Bacillus subtilis. Furthermore, the antitumor and antiviral activity of separated dandelion biological products was evaluated.
PB  - Institution of Chemical Engineers
T2  - Food and Bioproducts Processing
T1  - Phenolic, tocopherol, and essential fatty acid-rich extracts from dandelion seeds: Chemical composition and biological activity
EP  - 81
SP  - 70
VL  - 142
DO  - 10.1016/j.fbp.2023.09.005
ER  - 

@article{
author = "Milovanović, Stoja and Grzegorczyk, Agnieszka and Świątek, Łukasz and Boguszewska, Anastazja and Kowalski, Rafał and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2023",
abstract = "Dandelion is a sustainable biomass that has occasional use in the food and pharmaceutical industry. However, commercial use of its extracts is restricted by the lack of systematic information on safety, composition, and biological activity. To broaden dandelion use, the present study brings new knowledge on extracts from dandelion seeds that could find application as safe and functional food ingredients. The employed two-step supercritical fluid extraction process, followed by the conventional extraction, enabled the separation of extracts with increased content of phenolics, tocopherols, and essential fatty acids. Those compounds were found to be the major components of dandelion extracts, reaching high values up to 799 mg GE/g, 249 mg α-TE/g, and 75%, respectively. The extracts also contained significant amounts of flavonoids (up to 20.5 mg QE/g) and pigments (chlorophyll A up to 1.0 g/kg, chlorophyll B up to 0.3 g/kg, and carotenoids up to 0.1 g/kg). This study revealed new bioproducts that express strong antioxidant (reaching IC50 values up to 132.5 μg/mL) and antimicrobial activity (with minimum inhibitory concentrations up to 80.0 μg/mL). Among the tested 23 microbial strains, the extracts exhibited the strongest activity against food-borne pathogens Micrococcus luteus and Bacillus subtilis. Furthermore, the antitumor and antiviral activity of separated dandelion biological products was evaluated.",
publisher = "Institution of Chemical Engineers",
journal = "Food and Bioproducts Processing",
title = "Phenolic, tocopherol, and essential fatty acid-rich extracts from dandelion seeds: Chemical composition and biological activity",
pages = "81-70",
volume = "142",
doi = "10.1016/j.fbp.2023.09.005"
}

Milovanović, S., Grzegorczyk, A., Świątek, Ł., Boguszewska, A., Kowalski, R., Tyśkiewicz, K.,& Konkol, M.. (2023). Phenolic, tocopherol, and essential fatty acid-rich extracts from dandelion seeds: Chemical composition and biological activity. in Food and Bioproducts Processing
Institution of Chemical Engineers., 142, 70-81.
https://doi.org/10.1016/j.fbp.2023.09.005

Milovanović S, Grzegorczyk A, Świątek Ł, Boguszewska A, Kowalski R, Tyśkiewicz K, Konkol M. Phenolic, tocopherol, and essential fatty acid-rich extracts from dandelion seeds: Chemical composition and biological activity. in Food and Bioproducts Processing. 2023;142:70-81.
doi:10.1016/j.fbp.2023.09.005 .

Milovanović, Stoja, Grzegorczyk, Agnieszka, Świątek, Łukasz, Boguszewska, Anastazja, Kowalski, Rafał, Tyśkiewicz, Katarzyna, Konkol, Marcin, "Phenolic, tocopherol, and essential fatty acid-rich extracts from dandelion seeds: Chemical composition and biological activity" in Food and Bioproducts Processing, 142 (2023):70-81,
https://doi.org/10.1016/j.fbp.2023.09.005 . .

TY  - CONF
AU  - Milovanović, Stoja
AU  - Grzegorczyk, Agnieszka
AU  - Świątek, Łukasz
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6894
AB  - The main aim of this study was to evaluate chamomile seeds as a new source of valuable bioactive compounds. For this purpose, a green extraction technique was employed with supercritical carbon dioxide (sc-CO2) as a solvent and ethanol as a co-solvent. The extracts from chamomile seeds with an average particle size of 0.20 mm were extracted at pressures of 30 and 45 MPa and temperatures of 40 and 60 °C. The composition of extracts was investigated by a gas chromatography-mass spectrometry analytical method as well as Folin−Ciocalteu assay (for total phenolic content) and total flavonoid content test. The antioxidant activity of obtained chamomile extracts was evaluated using DPPH radical. The antimicrobial effect of extracts was tested on yeasts, Gram-positive, and Gram-negative bacteria while the cytotoxic effect was evaluated on normal and cancer cells. The extraction process parameters were evaluated and results showed a significant effect of operating pressure, temperature, and the addition of co-solvent on the amount of separated chamomile extract and its composition. The extraction yield ranged from 2.1 to 5.4% and extracts contained high amounts of unsaturated fatty acids. The addition of ethanol as a co-solvent (40 w/w%) had a slight influence on total phenolic and flavonoid contents increasing their values from 29.1 to 31.5 mg GAE/g and from 2.1 to 4.2 mg QE/g, respectively. The obtained supercritical chamomile extracts showed antioxidant activity achieving values of IC50 around 10 mg/mL. The antimicrobial activity of extracts was most pronounced against Bacillus subtilis. Extracts did not show cytotoxic activity against normal cells. Overall, results indicated a significant potential of extracts from chamomile seeds, produced in an environmentally friendly manner, to be used as a functional food or phytopharmaceuticals.
PB  - Valladolid : The lnstitute of Bioeconomy of University of Valladolid
C3  - Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain
T1  - The separation of biologically active extracts from chamomile seeds
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6894
ER  - 

@conference{
author = "Milovanović, Stoja and Grzegorczyk, Agnieszka and Świątek, Łukasz and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2023",
abstract = "The main aim of this study was to evaluate chamomile seeds as a new source of valuable bioactive compounds. For this purpose, a green extraction technique was employed with supercritical carbon dioxide (sc-CO2) as a solvent and ethanol as a co-solvent. The extracts from chamomile seeds with an average particle size of 0.20 mm were extracted at pressures of 30 and 45 MPa and temperatures of 40 and 60 °C. The composition of extracts was investigated by a gas chromatography-mass spectrometry analytical method as well as Folin−Ciocalteu assay (for total phenolic content) and total flavonoid content test. The antioxidant activity of obtained chamomile extracts was evaluated using DPPH radical. The antimicrobial effect of extracts was tested on yeasts, Gram-positive, and Gram-negative bacteria while the cytotoxic effect was evaluated on normal and cancer cells. The extraction process parameters were evaluated and results showed a significant effect of operating pressure, temperature, and the addition of co-solvent on the amount of separated chamomile extract and its composition. The extraction yield ranged from 2.1 to 5.4% and extracts contained high amounts of unsaturated fatty acids. The addition of ethanol as a co-solvent (40 w/w%) had a slight influence on total phenolic and flavonoid contents increasing their values from 29.1 to 31.5 mg GAE/g and from 2.1 to 4.2 mg QE/g, respectively. The obtained supercritical chamomile extracts showed antioxidant activity achieving values of IC50 around 10 mg/mL. The antimicrobial activity of extracts was most pronounced against Bacillus subtilis. Extracts did not show cytotoxic activity against normal cells. Overall, results indicated a significant potential of extracts from chamomile seeds, produced in an environmentally friendly manner, to be used as a functional food or phytopharmaceuticals.",
publisher = "Valladolid : The lnstitute of Bioeconomy of University of Valladolid",
journal = "Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain",
title = "The separation of biologically active extracts from chamomile seeds",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6894"
}

Milovanović, S., Grzegorczyk, A., Świątek, Ł., Tyśkiewicz, K.,& Konkol, M.. (2023). The separation of biologically active extracts from chamomile seeds. in Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain
Valladolid : The lnstitute of Bioeconomy of University of Valladolid..
https://hdl.handle.net/21.15107/rcub_technorep_6894

Milovanović S, Grzegorczyk A, Świątek Ł, Tyśkiewicz K, Konkol M. The separation of biologically active extracts from chamomile seeds. in Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6894 .

Milovanović, Stoja, Grzegorczyk, Agnieszka, Świątek, Łukasz, Tyśkiewicz, Katarzyna, Konkol, Marcin, "The separation of biologically active extracts from chamomile seeds" in Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6894 .

TY  - CONF
AU  - Stamenić, Marko
AU  - Milovanović, Stoja
AU  - Kowalski, Rafał
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6908
AB  - Investigations of the process of supercritical fluid extraction (SFE) from dandelion were so far mainly
focused on extractions from herb, leaves and root [1-3]. In the work presented here, the extraction process
from seeds was investigated on a laboratory and semi-industrial scale equipment. The milled plant material
with an average particle size of 0.41 ± 0.02 mm was used in all experiments. All SFEs were conducted with
carbon-dioxide in supercritical state (SCCO2) as a solvent on pressure of 450 bar and temperature of 60°C, as
these parameters were previously shown to be optimal for obtaining high-value extracts from dandelion
seeds [4]. Experiments were conducted on laboratory (dia.=7.7 cm, vol=0.56 l, CO2 flow=11 kg/h) and semiindustrial
(dia.=23.5 cm, vol.=397 l, CO2 flow=240 kg/h) scale equipment. On both scales several
experiments were conducted with initial mass of the plant material (Ms) being the only variable parameter:
laboratory scale Ms=26–104 g; semi-industrial scale Ms=2.2–8.8 kg. Fractions of extract were collected
during the extractions in order to obtain data on the kinetics of the SFE processes.
The results of the performed
experiments are depicted in Figure 1.
It can be seen that for both scales the
rate of SFE, as well as the final yield,
increases with increase of the mass
of plant material, indicating that with
the selected conditions, namely
flowrate of CO2, the residence time
was not long enough to allow
saturation of the CO2 in the first part
of the process, nor to enable
extraction of all available extract.
Similar trends on both scales can be
perceived as a good basis for further
investigations aiming at scale-up of the process of SFE from dandelion seeds. Experiments with larger
packed bed height, which would enable higher residence times, should be conducted in order to achieve
saturation at least in the first part of the SFE process. Mathematical models of the SFE process, supported by
models for estimation of the kinetic parameters, should be applied to gain full insight into the
phenomenology of the SFE process.
PB  - Budapest : University of Technology and Economics
C3  - Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
T1  - Supercritical fluid extraction from dandelion seeds
SP  - 136
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6908
ER  - 

@conference{
author = "Stamenić, Marko and Milovanović, Stoja and Kowalski, Rafał and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2023",
abstract = "Investigations of the process of supercritical fluid extraction (SFE) from dandelion were so far mainly
focused on extractions from herb, leaves and root [1-3]. In the work presented here, the extraction process
from seeds was investigated on a laboratory and semi-industrial scale equipment. The milled plant material
with an average particle size of 0.41 ± 0.02 mm was used in all experiments. All SFEs were conducted with
carbon-dioxide in supercritical state (SCCO2) as a solvent on pressure of 450 bar and temperature of 60°C, as
these parameters were previously shown to be optimal for obtaining high-value extracts from dandelion
seeds [4]. Experiments were conducted on laboratory (dia.=7.7 cm, vol=0.56 l, CO2 flow=11 kg/h) and semiindustrial
(dia.=23.5 cm, vol.=397 l, CO2 flow=240 kg/h) scale equipment. On both scales several
experiments were conducted with initial mass of the plant material (Ms) being the only variable parameter:
laboratory scale Ms=26–104 g; semi-industrial scale Ms=2.2–8.8 kg. Fractions of extract were collected
during the extractions in order to obtain data on the kinetics of the SFE processes.
The results of the performed
experiments are depicted in Figure 1.
It can be seen that for both scales the
rate of SFE, as well as the final yield,
increases with increase of the mass
of plant material, indicating that with
the selected conditions, namely
flowrate of CO2, the residence time
was not long enough to allow
saturation of the CO2 in the first part
of the process, nor to enable
extraction of all available extract.
Similar trends on both scales can be
perceived as a good basis for further
investigations aiming at scale-up of the process of SFE from dandelion seeds. Experiments with larger
packed bed height, which would enable higher residence times, should be conducted in order to achieve
saturation at least in the first part of the SFE process. Mathematical models of the SFE process, supported by
models for estimation of the kinetic parameters, should be applied to gain full insight into the
phenomenology of the SFE process.",
publisher = "Budapest : University of Technology and Economics",
journal = "Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023",
title = "Supercritical fluid extraction from dandelion seeds",
pages = "136",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6908"
}

Stamenić, M., Milovanović, S., Kowalski, R., Tyśkiewicz, K.,& Konkol, M.. (2023). Supercritical fluid extraction from dandelion seeds. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
Budapest : University of Technology and Economics., 136.
https://hdl.handle.net/21.15107/rcub_technorep_6908

Stamenić M, Milovanović S, Kowalski R, Tyśkiewicz K, Konkol M. Supercritical fluid extraction from dandelion seeds. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023. 2023;:136.
https://hdl.handle.net/21.15107/rcub_technorep_6908 .

Stamenić, Marko, Milovanović, Stoja, Kowalski, Rafał, Tyśkiewicz, Katarzyna, Konkol, Marcin, "Supercritical fluid extraction from dandelion seeds" in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 (2023):136,
https://hdl.handle.net/21.15107/rcub_technorep_6908 .

TY  - CONF
AU  - Milovanović, Stoja
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6906
AB  - Interest in the separation of green tea extracts continues to grow due to its significant bioactivity, wide
applicability as well as a large number of green tea spices, variety of extraction techniques, and lack of
systematic data. The present early stage work was intended to estimate the effect of temperature and co-solvent
addition on an amount of separated constituents from green tea (gunpowder tea variety, China) during the
supercritical carbon dioxide (sc-CO2) extraction process.
For this purpose, the temperature of the supercritical extraction process was increased up to 90 °C at a constant
pressure of 30 MPa. After the selection of appropriate conditions, co-solvents (ethanol, ethanol/water, and
water) were added to sc-CO2. Obtained extracts were analyzed regarding total phenolic content (TPC), total
flavonoid content (TFC), DPPH radical scavenging activity, total chlorophyll, and total carotenoid content.
The results of the supercritical extraction process were compared with the conventional extraction process
(extraction using Soxhlet apparatus).
The results showed that a linear decrease in the density of neat sc-CO2 led to a linear increase in extraction
yields up to 5.5%. The addition of co-solvents had a variable effect on the amount of separated extracts. While
it increased extraction yields at a lower temperature, it decreased extraction yields at a higher temperature.
An increase in temperature for the extraction process with neat sc-
CO2 also decreased TPC, TFC, chlorophylls, and carotenoids
content. The addition of co-solvents to sc-CO2 increased amounts
of tested compounds in obtained extracts. It was interesting to
notice a decrease in the amount of tested compounds with an
increase in water content during extractions. The highest values of
TPC and TFC detected were 136.5 mg GAE/g and 40.7 mg QE/g,
respectively. Extract solutions with a concentration of 20 mg/mL
inhibited up to 65% of DPPH radical. Chlorophyll A, chlorophyll
B, and carotenoids were detected in amounts up to 8.1 g/kg,
0.7 g/kg, and 0.5 g/kg of dry extract, respectively. Extracts
obtained by conventional extraction contained higher amounts of
TPC and TFC. On the other hand, those extracts contained lower
amounts of total chlorophylls and carotenoids.
Although supercritical extraction processes from green tea leaves have been performed for decades and its
industrial application is present, there are still many unknowns. To the best of our knowledge, this is the first
report in available literature on chlorophyll and carotenoid contents in supercritical extracts from green tea.
Also tested process parameters enabled the achievement of higher yields (for neat sc-CO2) as well as higher
TPC compared with previously reported ones for green tea supercritical extracts. Chemical analysis of obtained
extracts is pending.
PB  - Budapest : University of Technology and Economics
C3  - Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
T1  - Influence of supercritical carbon dioxide extraction process temperature and co-solvent addition on the quality of green tea leaves extracts
SP  - 123
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6906
ER  - 

@conference{
author = "Milovanović, Stoja and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2023",
abstract = "Interest in the separation of green tea extracts continues to grow due to its significant bioactivity, wide
applicability as well as a large number of green tea spices, variety of extraction techniques, and lack of
systematic data. The present early stage work was intended to estimate the effect of temperature and co-solvent
addition on an amount of separated constituents from green tea (gunpowder tea variety, China) during the
supercritical carbon dioxide (sc-CO2) extraction process.
For this purpose, the temperature of the supercritical extraction process was increased up to 90 °C at a constant
pressure of 30 MPa. After the selection of appropriate conditions, co-solvents (ethanol, ethanol/water, and
water) were added to sc-CO2. Obtained extracts were analyzed regarding total phenolic content (TPC), total
flavonoid content (TFC), DPPH radical scavenging activity, total chlorophyll, and total carotenoid content.
The results of the supercritical extraction process were compared with the conventional extraction process
(extraction using Soxhlet apparatus).
The results showed that a linear decrease in the density of neat sc-CO2 led to a linear increase in extraction
yields up to 5.5%. The addition of co-solvents had a variable effect on the amount of separated extracts. While
it increased extraction yields at a lower temperature, it decreased extraction yields at a higher temperature.
An increase in temperature for the extraction process with neat sc-
CO2 also decreased TPC, TFC, chlorophylls, and carotenoids
content. The addition of co-solvents to sc-CO2 increased amounts
of tested compounds in obtained extracts. It was interesting to
notice a decrease in the amount of tested compounds with an
increase in water content during extractions. The highest values of
TPC and TFC detected were 136.5 mg GAE/g and 40.7 mg QE/g,
respectively. Extract solutions with a concentration of 20 mg/mL
inhibited up to 65% of DPPH radical. Chlorophyll A, chlorophyll
B, and carotenoids were detected in amounts up to 8.1 g/kg,
0.7 g/kg, and 0.5 g/kg of dry extract, respectively. Extracts
obtained by conventional extraction contained higher amounts of
TPC and TFC. On the other hand, those extracts contained lower
amounts of total chlorophylls and carotenoids.
Although supercritical extraction processes from green tea leaves have been performed for decades and its
industrial application is present, there are still many unknowns. To the best of our knowledge, this is the first
report in available literature on chlorophyll and carotenoid contents in supercritical extracts from green tea.
Also tested process parameters enabled the achievement of higher yields (for neat sc-CO2) as well as higher
TPC compared with previously reported ones for green tea supercritical extracts. Chemical analysis of obtained
extracts is pending.",
publisher = "Budapest : University of Technology and Economics",
journal = "Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023",
title = "Influence of supercritical carbon dioxide extraction process temperature and co-solvent addition on the quality of green tea leaves extracts",
pages = "123",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6906"
}

Milovanović, S., Tyśkiewicz, K.,& Konkol, M.. (2023). Influence of supercritical carbon dioxide extraction process temperature and co-solvent addition on the quality of green tea leaves extracts. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
Budapest : University of Technology and Economics., 123.
https://hdl.handle.net/21.15107/rcub_technorep_6906

Milovanović S, Tyśkiewicz K, Konkol M. Influence of supercritical carbon dioxide extraction process temperature and co-solvent addition on the quality of green tea leaves extracts. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023. 2023;:123.
https://hdl.handle.net/21.15107/rcub_technorep_6906 .

Milovanović, Stoja, Tyśkiewicz, Katarzyna, Konkol, Marcin, "Influence of supercritical carbon dioxide extraction process temperature and co-solvent addition on the quality of green tea leaves extracts" in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 (2023):123,
https://hdl.handle.net/21.15107/rcub_technorep_6906 .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Lukić, Ivana
AU  - Horvat, Gabrijela
AU  - Novak, Zoran
AU  - Frerich, Sulamith
AU  - Petermann, Marcus
AU  - García-González, Carlos A.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6032
AB  - This review provides a concise overview of up-to-date developments in the processing of neat poly(lactic acid) (PLA), improvement in its properties, and preparation of advanced materials using a green medium (CO2 under elevated pressure). Pressurized CO2 in the dense and supercritical state is a superior alternative medium to organic solvents, as it is easily available, fully recyclable, has easily tunable properties, and can be completely removed from the final material without post-processing steps. This review summarizes the state of the art on PLA drying, impregnation, foaming, and particle generation by the employment of dense and supercritical CO2 for the development of new materials. An analysis of the effect of processing methods on the final material properties was focused on neat PLA and PLA with an addition of natural bioactive components. It was demonstrated that CO2-assisted processes enable the control of PLA properties, reduce operating times, and require less energy compared to conventional ones. The described environmentally friendly processing techniques and the versatility of PLA were employed for the preparation of foams, aerogels, scaffolds, microparticles, and nanoparticles, as well as bioactive materials. These PLA-based materials can find application in tissue engineering, drug delivery, active food packaging, compostable packaging, wastewater treatment, or thermal insulation, among others.
PB  - MDPI
T2  - Polymers
T1  - Green Processing of Neat Poly(lactic acid) Using Carbon Dioxide under Elevated Pressure for Preparation of Advanced Materials: A Review (2012–2022)
IS  - 4
SP  - 860
VL  - 15
DO  - 10.3390/polym15040860
ER  - 

@article{
author = "Milovanović, Stoja and Lukić, Ivana and Horvat, Gabrijela and Novak, Zoran and Frerich, Sulamith and Petermann, Marcus and García-González, Carlos A.",
year = "2023",
abstract = "This review provides a concise overview of up-to-date developments in the processing of neat poly(lactic acid) (PLA), improvement in its properties, and preparation of advanced materials using a green medium (CO2 under elevated pressure). Pressurized CO2 in the dense and supercritical state is a superior alternative medium to organic solvents, as it is easily available, fully recyclable, has easily tunable properties, and can be completely removed from the final material without post-processing steps. This review summarizes the state of the art on PLA drying, impregnation, foaming, and particle generation by the employment of dense and supercritical CO2 for the development of new materials. An analysis of the effect of processing methods on the final material properties was focused on neat PLA and PLA with an addition of natural bioactive components. It was demonstrated that CO2-assisted processes enable the control of PLA properties, reduce operating times, and require less energy compared to conventional ones. The described environmentally friendly processing techniques and the versatility of PLA were employed for the preparation of foams, aerogels, scaffolds, microparticles, and nanoparticles, as well as bioactive materials. These PLA-based materials can find application in tissue engineering, drug delivery, active food packaging, compostable packaging, wastewater treatment, or thermal insulation, among others.",
publisher = "MDPI",
journal = "Polymers",
title = "Green Processing of Neat Poly(lactic acid) Using Carbon Dioxide under Elevated Pressure for Preparation of Advanced Materials: A Review (2012–2022)",
number = "4",
pages = "860",
volume = "15",
doi = "10.3390/polym15040860"
}

Milovanović, S., Lukić, I., Horvat, G., Novak, Z., Frerich, S., Petermann, M.,& García-González, C. A.. (2023). Green Processing of Neat Poly(lactic acid) Using Carbon Dioxide under Elevated Pressure for Preparation of Advanced Materials: A Review (2012–2022). in Polymers
MDPI., 15(4), 860.
https://doi.org/10.3390/polym15040860

Milovanović S, Lukić I, Horvat G, Novak Z, Frerich S, Petermann M, García-González CA. Green Processing of Neat Poly(lactic acid) Using Carbon Dioxide under Elevated Pressure for Preparation of Advanced Materials: A Review (2012–2022). in Polymers. 2023;15(4):860.
doi:10.3390/polym15040860 .

Milovanović, Stoja, Lukić, Ivana, Horvat, Gabrijela, Novak, Zoran, Frerich, Sulamith, Petermann, Marcus, García-González, Carlos A., "Green Processing of Neat Poly(lactic acid) Using Carbon Dioxide under Elevated Pressure for Preparation of Advanced Materials: A Review (2012–2022)" in Polymers, 15, no. 4 (2023):860,
https://doi.org/10.3390/polym15040860 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Grzegorczyk, Agnieszka
AU  - Świątek, Łukasz
AU  - Grzęda, Anita
AU  - Dębczak, Agnieszka
AU  - Tyskiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6031
AB  - The main aim of this study was to evaluate the oils from chamomile seeds as a new source of bioactive compounds suitable for human consumption. A green extraction technique with supercritical carbon dioxide (sc-CO2) at pressures up to 450 bar and temperatures up to 60 °C was employed for the production of a high amount of biologically active oil. Additionally, exhausted waste material was re-extracted using sc-CO2 with the addition of ethanol. By optimization in operating pressure, temperature, production cost, fraction of milled seeds, and co-solvent addition, the amount of separated chamomile oil increased from 2.4 to 18.6% and the content of unsaturated fatty acids up to 88.7%. Oils contained α-bisabolol oxide A and B in amounts up to 1.4%. Linoleic acid was detected in an amount up to 711.1 mg/g and α-linolenic acid up to 27.5 mg/g. The total phenolic content in separated oil reached 80.4 mg GAE/g while the total flavonoid content reached 11.6 mg QE/g. The obtained chamomile oils showed antioxidant activity with an IC50 of up to 3.9 mg/mL. Among the 23 tested microorganisms, the antimicrobial activity of oils was the most pronounced against Gram-positive bacteria. The cytotoxic activity of oils was tested on normal and cancer-derived cell lines. Results indicated a significant potential for oil from chamomile seeds, produced in an eco-friendly manner, as a functional food.
PB  - Springer
T2  - Food and Bioprocess Technology
T1  - A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds
DO  - 10.1007/s11947-023-03038-9
ER  - 

@article{
author = "Milovanović, Stoja and Grzegorczyk, Agnieszka and Świątek, Łukasz and Grzęda, Anita and Dębczak, Agnieszka and Tyskiewicz, Katarzyna and Konkol, Marcin",
year = "2023",
abstract = "The main aim of this study was to evaluate the oils from chamomile seeds as a new source of bioactive compounds suitable for human consumption. A green extraction technique with supercritical carbon dioxide (sc-CO2) at pressures up to 450 bar and temperatures up to 60 °C was employed for the production of a high amount of biologically active oil. Additionally, exhausted waste material was re-extracted using sc-CO2 with the addition of ethanol. By optimization in operating pressure, temperature, production cost, fraction of milled seeds, and co-solvent addition, the amount of separated chamomile oil increased from 2.4 to 18.6% and the content of unsaturated fatty acids up to 88.7%. Oils contained α-bisabolol oxide A and B in amounts up to 1.4%. Linoleic acid was detected in an amount up to 711.1 mg/g and α-linolenic acid up to 27.5 mg/g. The total phenolic content in separated oil reached 80.4 mg GAE/g while the total flavonoid content reached 11.6 mg QE/g. The obtained chamomile oils showed antioxidant activity with an IC50 of up to 3.9 mg/mL. Among the 23 tested microorganisms, the antimicrobial activity of oils was the most pronounced against Gram-positive bacteria. The cytotoxic activity of oils was tested on normal and cancer-derived cell lines. Results indicated a significant potential for oil from chamomile seeds, produced in an eco-friendly manner, as a functional food.",
publisher = "Springer",
journal = "Food and Bioprocess Technology",
title = "A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds",
doi = "10.1007/s11947-023-03038-9"
}

Milovanović, S., Grzegorczyk, A., Świątek, Ł., Grzęda, A., Dębczak, A., Tyskiewicz, K.,& Konkol, M.. (2023). A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds. in Food and Bioprocess Technology
Springer..
https://doi.org/10.1007/s11947-023-03038-9

Milovanović S, Grzegorczyk A, Świątek Ł, Grzęda A, Dębczak A, Tyskiewicz K, Konkol M. A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds. in Food and Bioprocess Technology. 2023;.
doi:10.1007/s11947-023-03038-9 .

Milovanović, Stoja, Grzegorczyk, Agnieszka, Świątek, Łukasz, Grzęda, Anita, Dębczak, Agnieszka, Tyskiewicz, Katarzyna, Konkol, Marcin, "A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds" in Food and Bioprocess Technology (2023),
https://doi.org/10.1007/s11947-023-03038-9 . .

TY  - CONF
AU  - Lukić, Ivana
AU  - Milovanović, Stoja
AU  - Tadić, Vanja
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6791
AB  - Bilberries (Vaccinium myrtillus) have been used in traditional medicine to reduce
inflammation and protect against diseases associated with oxidative stress, such as
cardiovascular disease, diabetes, and age-related cognitive decline, as well as a
remedy for eye conditions. The biological activity of bilberries is ascribed mainly to
their high content of anthocyanins, but also to other valuable phytochemicals,
including flavonols, phenolic acids, and tannins [1,2]. The enhanced stability and
bioavailability of bioactive components from plant material could be accomplished
by their incorporation into a polymeric carrier, using the promising integrated
processes of supercritical fluid extraction (SFE) and supercritical solvent
impregnation (SSI). ...
PB  - Maribor : University of Maribor
PB  - Maribor : University Press
C3  - Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia
T1  - Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel
EP  - 109
SP  - 107
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6791
ER  - 

@conference{
author = "Lukić, Ivana and Milovanović, Stoja and Tadić, Vanja",
year = "2023",
abstract = "Bilberries (Vaccinium myrtillus) have been used in traditional medicine to reduce
inflammation and protect against diseases associated with oxidative stress, such as
cardiovascular disease, diabetes, and age-related cognitive decline, as well as a
remedy for eye conditions. The biological activity of bilberries is ascribed mainly to
their high content of anthocyanins, but also to other valuable phytochemicals,
including flavonols, phenolic acids, and tannins [1,2]. The enhanced stability and
bioavailability of bioactive components from plant material could be accomplished
by their incorporation into a polymeric carrier, using the promising integrated
processes of supercritical fluid extraction (SFE) and supercritical solvent
impregnation (SSI). ...",
publisher = "Maribor : University of Maribor, Maribor : University Press",
journal = "Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia",
title = "Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel",
pages = "109-107",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6791"
}

Lukić, I., Milovanović, S.,& Tadić, V.. (2023). Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel. in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia
Maribor : University of Maribor., 107-109.
https://hdl.handle.net/21.15107/rcub_technorep_6791

Lukić I, Milovanović S, Tadić V. Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel. in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia. 2023;:107-109.
https://hdl.handle.net/21.15107/rcub_technorep_6791 .

Lukić, Ivana, Milovanović, Stoja, Tadić, Vanja, "Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel" in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia (2023):107-109,
https://hdl.handle.net/21.15107/rcub_technorep_6791 .

TY  - CONF
AU  - Milovanović, Stoja
AU  - Lukić, Ivana
AU  - Marković, Darka
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6792
AB  - Natural compounds found in different plant extracts can show numerous biological
activities and be used as health promoters. For instance, thymol, carvacrol,
citronellol, and eugenol (found in extracts of thyme, oregano, lemongrass, and clove,
respectively) can be used as anti-inflammatory, antiviral, and antimicrobial agents [1-
4]. The high volatility of the mentioned natural bioactive compounds (NBCs), which
restricts their broader application, can be managed by the incorporation of NBCs
into polymer matrices. Therefore, intending to develop devices that release
antimicrobial substances in a controlled manner, this study tested the applicability
of the supercritical solvent impregnation (SSI) technique for the incorporation of
NBCs into starch aerogels. First, the hydrogels were prepared from corn starch. The
water in the hydrogels was replaced by ethanol, and the obtained alcogels were dried
using the supercritical drying (SCD) technique. The parameters of the aerogels`
preparation were optimized previously [5].Later, SSI was performed at 150 bar and
35 °C for 2 h. The obtained materials were analyzed using FTIR, to determine the
potential interactions between the NBCs and polymer that could determine the rate
of controlled release and antimicrobial activity. Controlled release of NBCs from the
aerogels was tested in phosphate-buffered saline solution at 37 °C. ...
PB  - Maribor : University of Maribor
PB  - Maribor : University Press
C3  - Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia
T1  - Supercritical Solvent Impregnation Technique for the Development of Antimicrobial Starch-based Aerogels
EP  - 165
SP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6792
ER  - 

@conference{
author = "Milovanović, Stoja and Lukić, Ivana and Marković, Darka",
year = "2023",
abstract = "Natural compounds found in different plant extracts can show numerous biological
activities and be used as health promoters. For instance, thymol, carvacrol,
citronellol, and eugenol (found in extracts of thyme, oregano, lemongrass, and clove,
respectively) can be used as anti-inflammatory, antiviral, and antimicrobial agents [1-
4]. The high volatility of the mentioned natural bioactive compounds (NBCs), which
restricts their broader application, can be managed by the incorporation of NBCs
into polymer matrices. Therefore, intending to develop devices that release
antimicrobial substances in a controlled manner, this study tested the applicability
of the supercritical solvent impregnation (SSI) technique for the incorporation of
NBCs into starch aerogels. First, the hydrogels were prepared from corn starch. The
water in the hydrogels was replaced by ethanol, and the obtained alcogels were dried
using the supercritical drying (SCD) technique. The parameters of the aerogels`
preparation were optimized previously [5].Later, SSI was performed at 150 bar and
35 °C for 2 h. The obtained materials were analyzed using FTIR, to determine the
potential interactions between the NBCs and polymer that could determine the rate
of controlled release and antimicrobial activity. Controlled release of NBCs from the
aerogels was tested in phosphate-buffered saline solution at 37 °C. ...",
publisher = "Maribor : University of Maribor, Maribor : University Press",
journal = "Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia",
title = "Supercritical Solvent Impregnation Technique for the Development of Antimicrobial Starch-based Aerogels",
pages = "165-163",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6792"
}

Milovanović, S., Lukić, I.,& Marković, D.. (2023). Supercritical Solvent Impregnation Technique for the Development of Antimicrobial Starch-based Aerogels. in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia
Maribor : University of Maribor., 163-165.
https://hdl.handle.net/21.15107/rcub_technorep_6792

Milovanović S, Lukić I, Marković D. Supercritical Solvent Impregnation Technique for the Development of Antimicrobial Starch-based Aerogels. in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia. 2023;:163-165.
https://hdl.handle.net/21.15107/rcub_technorep_6792 .

Milovanović, Stoja, Lukić, Ivana, Marković, Darka, "Supercritical Solvent Impregnation Technique for the Development of Antimicrobial Starch-based Aerogels" in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia (2023):163-165,
https://hdl.handle.net/21.15107/rcub_technorep_6792 .

TY  - GEN
AU  - Milovanović, Stoja
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6892
AB  - Green technologies refer to a type of technology that is environmentally friendly based on its production process or its supply chain (i.e resources). 
Green tech also refers to clean energy production, use of alternative fuels, and processes that are less harmful to the environment compared to ones with fossil fuels.
The focus of this presentation is:
1) Processing of polymers obtained from renewable resources that are recyclable and/or biodegradable, 
2) Use of environmentally friendly techniques for the preparation of advanced materials (dry porous forms).
PB  - Graz : University of Technology
T2  - The Training Course “GRETEC 2023 - Green Techno School”, 6.2.-10.2.2023, Graz University of Technology, Austria
T1  - From Polymeric Wet-Gels to Functional Materials: Drying and Post-Processing
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6892
ER  - 

@misc{
author = "Milovanović, Stoja",
year = "2023",
abstract = "Green technologies refer to a type of technology that is environmentally friendly based on its production process or its supply chain (i.e resources). 
Green tech also refers to clean energy production, use of alternative fuels, and processes that are less harmful to the environment compared to ones with fossil fuels.
The focus of this presentation is:
1) Processing of polymers obtained from renewable resources that are recyclable and/or biodegradable, 
2) Use of environmentally friendly techniques for the preparation of advanced materials (dry porous forms).",
publisher = "Graz : University of Technology",
journal = "The Training Course “GRETEC 2023 - Green Techno School”, 6.2.-10.2.2023, Graz University of Technology, Austria",
title = "From Polymeric Wet-Gels to Functional Materials: Drying and Post-Processing",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6892"
}

Milovanović, S.. (2023). From Polymeric Wet-Gels to Functional Materials: Drying and Post-Processing. in The Training Course “GRETEC 2023 - Green Techno School”, 6.2.-10.2.2023, Graz University of Technology, Austria
Graz : University of Technology..
https://hdl.handle.net/21.15107/rcub_technorep_6892

Milovanović S. From Polymeric Wet-Gels to Functional Materials: Drying and Post-Processing. in The Training Course “GRETEC 2023 - Green Techno School”, 6.2.-10.2.2023, Graz University of Technology, Austria. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6892 .

Milovanović, Stoja, "From Polymeric Wet-Gels to Functional Materials: Drying and Post-Processing" in The Training Course “GRETEC 2023 - Green Techno School”, 6.2.-10.2.2023, Graz University of Technology, Austria (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6892 .

TY  - GEN
AU  - Milovanović, Stoja
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6897
AB  - Supercritical carbon dioxide (sc-CO2) has remarkable attributes as a processing medium such as high diffusivity, low viscosity, non-flammability, and inertness. In addition, it can be easily and completely separated from the final material, as well as fully recycled. This sets up sc-CO2-assisted technologies apart from conventional methodologies reliant on organic solvents. Some of the most prominent techniques that employ sc-CO2 are supercritical drying and supercritical impregnation for the functionalization of polymeric nanofibers, textiles, films, foams, and aerogels. Supercritical drying can be employed for the separation of liquids from wet polymer gels and the production of highly porous dry aerogels, while supercritical impregnation can be employed for the incorporation of bioactive components into polymer matrices. Overall, sc-CO2 technologies pioneer the development of next-generation materials while meticulous physical and chemical characterizations underpin the scientific journey.
PB  - Krakow : Faculty of Metals Engineering and Industrial Computer Science, AGH University of Science and Technology
T2  - Faculty of Metals Engineering and Industrial Computer Science, AGH University of Science and Technology, 3rd October 2023, Krakow, Poland
T1  - Application of supercritical carbon dioxide for the processing of polymers in the form of nanofibers, textiles, films, foams, and aerogels
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6897
ER  - 

@misc{
author = "Milovanović, Stoja",
year = "2023",
abstract = "Supercritical carbon dioxide (sc-CO2) has remarkable attributes as a processing medium such as high diffusivity, low viscosity, non-flammability, and inertness. In addition, it can be easily and completely separated from the final material, as well as fully recycled. This sets up sc-CO2-assisted technologies apart from conventional methodologies reliant on organic solvents. Some of the most prominent techniques that employ sc-CO2 are supercritical drying and supercritical impregnation for the functionalization of polymeric nanofibers, textiles, films, foams, and aerogels. Supercritical drying can be employed for the separation of liquids from wet polymer gels and the production of highly porous dry aerogels, while supercritical impregnation can be employed for the incorporation of bioactive components into polymer matrices. Overall, sc-CO2 technologies pioneer the development of next-generation materials while meticulous physical and chemical characterizations underpin the scientific journey.",
publisher = "Krakow : Faculty of Metals Engineering and Industrial Computer Science, AGH University of Science and Technology",
journal = "Faculty of Metals Engineering and Industrial Computer Science, AGH University of Science and Technology, 3rd October 2023, Krakow, Poland",
title = "Application of supercritical carbon dioxide for the processing of polymers in the form of nanofibers, textiles, films, foams, and aerogels",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6897"
}

Milovanović, S.. (2023). Application of supercritical carbon dioxide for the processing of polymers in the form of nanofibers, textiles, films, foams, and aerogels. in Faculty of Metals Engineering and Industrial Computer Science, AGH University of Science and Technology, 3rd October 2023, Krakow, Poland
Krakow : Faculty of Metals Engineering and Industrial Computer Science, AGH University of Science and Technology..
https://hdl.handle.net/21.15107/rcub_technorep_6897

Milovanović S. Application of supercritical carbon dioxide for the processing of polymers in the form of nanofibers, textiles, films, foams, and aerogels. in Faculty of Metals Engineering and Industrial Computer Science, AGH University of Science and Technology, 3rd October 2023, Krakow, Poland. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6897 .

Milovanović, Stoja, "Application of supercritical carbon dioxide for the processing of polymers in the form of nanofibers, textiles, films, foams, and aerogels" in Faculty of Metals Engineering and Industrial Computer Science, AGH University of Science and Technology, 3rd October 2023, Krakow, Poland (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6897 .

TY  - PAT
AU  - Tadić, Vanja
AU  - Nešić, Ivana
AU  - Milenković, Marina
AU  - Martinović, Milica
AU  - Žugić, Ana
AU  - Maksimović, Svetolik
AU  - Stojanović, Sanja
AU  - Milovanović, Stoja
AU  - Krgović, Nemanja
AU  - Petrović, Slobodan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6900
T2  - Zavod za intelektualnu svojinu republike Srbije
T1  - Liposomski preparati za topikalnu primenu kod osoba sa hiperglikemijom
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6900
ER  - 

@misc{
author = "Tadić, Vanja and Nešić, Ivana and Milenković, Marina and Martinović, Milica and Žugić, Ana and Maksimović, Svetolik and Stojanović, Sanja and Milovanović, Stoja and Krgović, Nemanja and Petrović, Slobodan",
year = "2023",
journal = "Zavod za intelektualnu svojinu republike Srbije",
title = "Liposomski preparati za topikalnu primenu kod osoba sa hiperglikemijom",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6900"
}

Tadić, V., Nešić, I., Milenković, M., Martinović, M., Žugić, A., Maksimović, S., Stojanović, S., Milovanović, S., Krgović, N.,& Petrović, S.. (2023). Liposomski preparati za topikalnu primenu kod osoba sa hiperglikemijom. in Zavod za intelektualnu svojinu republike Srbije.
https://hdl.handle.net/21.15107/rcub_technorep_6900

Tadić V, Nešić I, Milenković M, Martinović M, Žugić A, Maksimović S, Stojanović S, Milovanović S, Krgović N, Petrović S. Liposomski preparati za topikalnu primenu kod osoba sa hiperglikemijom. in Zavod za intelektualnu svojinu republike Srbije. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6900 .

Tadić, Vanja, Nešić, Ivana, Milenković, Marina, Martinović, Milica, Žugić, Ana, Maksimović, Svetolik, Stojanović, Sanja, Milovanović, Stoja, Krgović, Nemanja, Petrović, Slobodan, "Liposomski preparati za topikalnu primenu kod osoba sa hiperglikemijom" in Zavod za intelektualnu svojinu republike Srbije (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6900 .

TY  - JOUR
AU  - Pajnik, Jelena
AU  - Dikić, Jelena
AU  - Milovanović, Stoja
AU  - Milošević, Milena
AU  - Jevtić, Sanja
AU  - Lukić, Ivana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5085
AB  - Chitosan/gelatin and chitosan/gelatin/zeolite films prepared by solvent casting method were
impregnated with a mixture of thymol and carvacrol using a green solvent, supercritical carbon 
dioxide at 35 °C and 30 MPa, during 18 h. Proposed method enabled preparation of biocompatible 
and biodegradable blends with strong antioxidant and antibacterial activity, whereby amounts of 
loaded thymol/carvacrol mixture were in the range from 3.3-6%. After initial burst release, both 
types of films exhibited gradual release of bioactive compounds, with around 72 and 96% of 
impregnated thymol/carvacrol mixture released in water and PBS (pH 7.4) during tested period of 10 days, respectively. Results of water vapor transmission rate (> 76 gm-2
day-1) confirmed that prepared composites are suitable for wound dressing application. Thermal analysis showed superior properties of prepared thymol/carvacrol loaded films compared to control samples. In addition, mechanical and structural properties, as well as solubility and swelling behavior of the obtained films were investigated in detail.
PB  - Wiley
T2  - Macromolecular Materials and Engineering
T1  - Zeolite/chitosan/gelatin films: preparation, supercritical CO2 processing, characterization and bioactivity
EP  - 13
IS  - 4
SP  - 1
VL  - 307
DO  - 10.1002/mame.202200009
ER  - 

@article{
author = "Pajnik, Jelena and Dikić, Jelena and Milovanović, Stoja and Milošević, Milena and Jevtić, Sanja and Lukić, Ivana",
year = "2022",
abstract = "Chitosan/gelatin and chitosan/gelatin/zeolite films prepared by solvent casting method were
impregnated with a mixture of thymol and carvacrol using a green solvent, supercritical carbon 
dioxide at 35 °C and 30 MPa, during 18 h. Proposed method enabled preparation of biocompatible 
and biodegradable blends with strong antioxidant and antibacterial activity, whereby amounts of 
loaded thymol/carvacrol mixture were in the range from 3.3-6%. After initial burst release, both 
types of films exhibited gradual release of bioactive compounds, with around 72 and 96% of 
impregnated thymol/carvacrol mixture released in water and PBS (pH 7.4) during tested period of 10 days, respectively. Results of water vapor transmission rate (> 76 gm-2
day-1) confirmed that prepared composites are suitable for wound dressing application. Thermal analysis showed superior properties of prepared thymol/carvacrol loaded films compared to control samples. In addition, mechanical and structural properties, as well as solubility and swelling behavior of the obtained films were investigated in detail.",
publisher = "Wiley",
journal = "Macromolecular Materials and Engineering",
title = "Zeolite/chitosan/gelatin films: preparation, supercritical CO2 processing, characterization and bioactivity",
pages = "13-1",
number = "4",
volume = "307",
doi = "10.1002/mame.202200009"
}

Pajnik, J., Dikić, J., Milovanović, S., Milošević, M., Jevtić, S.,& Lukić, I.. (2022). Zeolite/chitosan/gelatin films: preparation, supercritical CO2 processing, characterization and bioactivity. in Macromolecular Materials and Engineering
Wiley., 307(4), 1-13.
https://doi.org/10.1002/mame.202200009

Pajnik J, Dikić J, Milovanović S, Milošević M, Jevtić S, Lukić I. Zeolite/chitosan/gelatin films: preparation, supercritical CO2 processing, characterization and bioactivity. in Macromolecular Materials and Engineering. 2022;307(4):1-13.
doi:10.1002/mame.202200009 .

Pajnik, Jelena, Dikić, Jelena, Milovanović, Stoja, Milošević, Milena, Jevtić, Sanja, Lukić, Ivana, "Zeolite/chitosan/gelatin films: preparation, supercritical CO2 processing, characterization and bioactivity" in Macromolecular Materials and Engineering, 307, no. 4 (2022):1-13,
https://doi.org/10.1002/mame.202200009 . .

TY  - JOUR
AU  - Pajnik, Jelena
AU  - Dikić, Jelena
AU  - Milovanović, Stoja
AU  - Milošević, Milena
AU  - Jevtić, Sanja
AU  - Lukić, Ivana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5086
AB  - Chitosan/gelatin and chitosan/gelatin/zeolite films prepared by solvent casting method were impregnated with a mixture of thymol and carvacrol using a green solvent, supercritical carbon dioxide at 35 °C and 30 MPa, during 18 h. Proposed method enabled preparation of biocompatible and biodegradable blends with strong antioxidant and antibacterial activity, whereby amounts of loaded thymol/carvacrol mixture were in the range from 3.3-6%. After initial burst release, both types of films exhibited gradual release of bioactive compounds, with around 72 and 96% of impregnated thymol/carvacrol mixture released in water and PBS (pH 7.4) during tested period of 10 days, respectively. Results of water vapor transmission rate (> 76 gm-2 day-1) confirmed that prepared composites are suitable for wound dressing application. Thermal analysis showed superior properties of prepared thymol/carvacrol loaded films compared to control samples. In addition, mechanical and structural properties, as well as solubility and swelling behavior of the obtained films were investigated in detail.
PB  - Wiley
T2  - Macromolecular Materials and Engineering
T1  - Zeolite/Chitosan/Gelatin Films: Preparation, SupercriticalCO2Processing, Characterization, and Bioactivity
EP  - 13
IS  - 4
SP  - 1
VL  - 307
DO  - 10.1002/mame.202200009
ER  - 

@article{
author = "Pajnik, Jelena and Dikić, Jelena and Milovanović, Stoja and Milošević, Milena and Jevtić, Sanja and Lukić, Ivana",
year = "2022",
abstract = "Chitosan/gelatin and chitosan/gelatin/zeolite films prepared by solvent casting method were impregnated with a mixture of thymol and carvacrol using a green solvent, supercritical carbon dioxide at 35 °C and 30 MPa, during 18 h. Proposed method enabled preparation of biocompatible and biodegradable blends with strong antioxidant and antibacterial activity, whereby amounts of loaded thymol/carvacrol mixture were in the range from 3.3-6%. After initial burst release, both types of films exhibited gradual release of bioactive compounds, with around 72 and 96% of impregnated thymol/carvacrol mixture released in water and PBS (pH 7.4) during tested period of 10 days, respectively. Results of water vapor transmission rate (> 76 gm-2 day-1) confirmed that prepared composites are suitable for wound dressing application. Thermal analysis showed superior properties of prepared thymol/carvacrol loaded films compared to control samples. In addition, mechanical and structural properties, as well as solubility and swelling behavior of the obtained films were investigated in detail.",
publisher = "Wiley",
journal = "Macromolecular Materials and Engineering",
title = "Zeolite/Chitosan/Gelatin Films: Preparation, SupercriticalCO2Processing, Characterization, and Bioactivity",
pages = "13-1",
number = "4",
volume = "307",
doi = "10.1002/mame.202200009"
}

Pajnik, J., Dikić, J., Milovanović, S., Milošević, M., Jevtić, S.,& Lukić, I.. (2022). Zeolite/Chitosan/Gelatin Films: Preparation, SupercriticalCO2Processing, Characterization, and Bioactivity. in Macromolecular Materials and Engineering
Wiley., 307(4), 1-13.
https://doi.org/10.1002/mame.202200009

Pajnik J, Dikić J, Milovanović S, Milošević M, Jevtić S, Lukić I. Zeolite/Chitosan/Gelatin Films: Preparation, SupercriticalCO2Processing, Characterization, and Bioactivity. in Macromolecular Materials and Engineering. 2022;307(4):1-13.
doi:10.1002/mame.202200009 .

Pajnik, Jelena, Dikić, Jelena, Milovanović, Stoja, Milošević, Milena, Jevtić, Sanja, Lukić, Ivana, "Zeolite/Chitosan/Gelatin Films: Preparation, SupercriticalCO2Processing, Characterization, and Bioactivity" in Macromolecular Materials and Engineering, 307, no. 4 (2022):1-13,
https://doi.org/10.1002/mame.202200009 . .

TY  - JOUR
AU  - Lučić Škorić, Marija
AU  - Milovanović, Stoja
AU  - Žižović, Irena
AU  - Ortega-Toro, Rodrigo
AU  - Santagata, Gabriella
AU  - Malinconico, Mario
AU  - Kalagasidis Krušić, Melina
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5243
AB  - The aim of the present study was to investigate starch-based materials, prepared in an environmentally friendly way and from renewable resources, suitable for the development of biodegradable active food packaging. For this purpose, a bioactive compound (thymol) was incorporated into thermoplastic starch (TPS) and a TPS blend with poly (ε-caprolactone) (TPS–PCL) by the supercritical CO2 (scCO2) impregnation process. Impregnation experiments with scCO2 were carried out at a pressure of 30 MPa and temperatures in the range of 40–100 °C during 1 to 20 h. The structural, morphological, and thermal properties of the obtained materials were comprehensively evaluated. Bioactive component release kinetic studies were performed in water at 6 °C and 25 °C. It was shown that the scCO2 impregnation process could be successfully employed for thymol loading into TPS and TPS–PCL. The process was significantly influenced by the operating temperature and time as well as content of PCL. The samples showed a controlled release of thymol within seven days with a higher amount of released thymol from the TPS–PCL blend. The obtained materials are solvent-free and release the bioactive component in a controlled manner.
PB  - MDPI
T2  - Polymers
T1  - Supercritical CO2 Impregnation of Thymol in Thermoplastic Starch-Based Blends: Chemico-Physical Properties and Release Kinetics
IS  - 20
SP  - 4360
VL  - 14
DO  - 10.3390/polym14204360
ER  - 

@article{
author = "Lučić Škorić, Marija and Milovanović, Stoja and Žižović, Irena and Ortega-Toro, Rodrigo and Santagata, Gabriella and Malinconico, Mario and Kalagasidis Krušić, Melina",
year = "2022",
abstract = "The aim of the present study was to investigate starch-based materials, prepared in an environmentally friendly way and from renewable resources, suitable for the development of biodegradable active food packaging. For this purpose, a bioactive compound (thymol) was incorporated into thermoplastic starch (TPS) and a TPS blend with poly (ε-caprolactone) (TPS–PCL) by the supercritical CO2 (scCO2) impregnation process. Impregnation experiments with scCO2 were carried out at a pressure of 30 MPa and temperatures in the range of 40–100 °C during 1 to 20 h. The structural, morphological, and thermal properties of the obtained materials were comprehensively evaluated. Bioactive component release kinetic studies were performed in water at 6 °C and 25 °C. It was shown that the scCO2 impregnation process could be successfully employed for thymol loading into TPS and TPS–PCL. The process was significantly influenced by the operating temperature and time as well as content of PCL. The samples showed a controlled release of thymol within seven days with a higher amount of released thymol from the TPS–PCL blend. The obtained materials are solvent-free and release the bioactive component in a controlled manner.",
publisher = "MDPI",
journal = "Polymers",
title = "Supercritical CO2 Impregnation of Thymol in Thermoplastic Starch-Based Blends: Chemico-Physical Properties and Release Kinetics",
number = "20",
pages = "4360",
volume = "14",
doi = "10.3390/polym14204360"
}

Lučić Škorić, M., Milovanović, S., Žižović, I., Ortega-Toro, R., Santagata, G., Malinconico, M.,& Kalagasidis Krušić, M.. (2022). Supercritical CO2 Impregnation of Thymol in Thermoplastic Starch-Based Blends: Chemico-Physical Properties and Release Kinetics. in Polymers
MDPI., 14(20), 4360.
https://doi.org/10.3390/polym14204360

Lučić Škorić M, Milovanović S, Žižović I, Ortega-Toro R, Santagata G, Malinconico M, Kalagasidis Krušić M. Supercritical CO2 Impregnation of Thymol in Thermoplastic Starch-Based Blends: Chemico-Physical Properties and Release Kinetics. in Polymers. 2022;14(20):4360.
doi:10.3390/polym14204360 .

Lučić Škorić, Marija, Milovanović, Stoja, Žižović, Irena, Ortega-Toro, Rodrigo, Santagata, Gabriella, Malinconico, Mario, Kalagasidis Krušić, Melina, "Supercritical CO2 Impregnation of Thymol in Thermoplastic Starch-Based Blends: Chemico-Physical Properties and Release Kinetics" in Polymers, 14, no. 20 (2022):4360,
https://doi.org/10.3390/polym14204360 . .

TY  - JOUR
AU  - Pajnik, Jelena
AU  - Milovanović, Stoja
AU  - Stojanović, Dušica
AU  - Dimitrijević-Branković, Suzana
AU  - Janković-Častvan, Ivona
AU  - Uskoković, Petar
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4979
AB  - Outbreak of surgical infections and bacterial resistance against synthetic antibiotic has prompted research towards development of antibacterial medical devices. For that purpose, absorbable surgical sutures were impregnated with natural compound thymol using an environmentally friendly medium. The supercritical solvent impregnation process was performed at 35 degrees C and 10 MPa during 1, 2, 3 and 6 h, resulting in thymol loadings of 3.9-5.6%. All tested samples expressed antibacterial activity against E. coli and S. aureus. Release tests in PBS at 37 degrees C showed that impregnated material released loaded thymol in a gradual manner. While sutures impregnated during 6 h released complete amount of loaded thymol during 6 days, shorter impregnation process resulted in sutures that retained significant thymol amounts (similar to 20-40%) after 10 days of release test. The effect of proposed process on final material properties and its application were explained in detail using characterization techniques SEM, FTIR, DSC and DMA.
PB  - Elsevier B.V.
T2  - Journal of Supercritical Fluids
T1  - Utilization of supercritical carbon dioxide for development of antibacterial surgical sutures
SP  - 105490
VL  - 181
DO  - 10.1016/j.supflu.2021.105490
ER  - 

@article{
author = "Pajnik, Jelena and Milovanović, Stoja and Stojanović, Dušica and Dimitrijević-Branković, Suzana and Janković-Častvan, Ivona and Uskoković, Petar",
year = "2022",
abstract = "Outbreak of surgical infections and bacterial resistance against synthetic antibiotic has prompted research towards development of antibacterial medical devices. For that purpose, absorbable surgical sutures were impregnated with natural compound thymol using an environmentally friendly medium. The supercritical solvent impregnation process was performed at 35 degrees C and 10 MPa during 1, 2, 3 and 6 h, resulting in thymol loadings of 3.9-5.6%. All tested samples expressed antibacterial activity against E. coli and S. aureus. Release tests in PBS at 37 degrees C showed that impregnated material released loaded thymol in a gradual manner. While sutures impregnated during 6 h released complete amount of loaded thymol during 6 days, shorter impregnation process resulted in sutures that retained significant thymol amounts (similar to 20-40%) after 10 days of release test. The effect of proposed process on final material properties and its application were explained in detail using characterization techniques SEM, FTIR, DSC and DMA.",
publisher = "Elsevier B.V.",
journal = "Journal of Supercritical Fluids",
title = "Utilization of supercritical carbon dioxide for development of antibacterial surgical sutures",
pages = "105490",
volume = "181",
doi = "10.1016/j.supflu.2021.105490"
}

Pajnik, J., Milovanović, S., Stojanović, D., Dimitrijević-Branković, S., Janković-Častvan, I.,& Uskoković, P.. (2022). Utilization of supercritical carbon dioxide for development of antibacterial surgical sutures. in Journal of Supercritical Fluids
Elsevier B.V.., 181, 105490.
https://doi.org/10.1016/j.supflu.2021.105490

Pajnik J, Milovanović S, Stojanović D, Dimitrijević-Branković S, Janković-Častvan I, Uskoković P. Utilization of supercritical carbon dioxide for development of antibacterial surgical sutures. in Journal of Supercritical Fluids. 2022;181:105490.
doi:10.1016/j.supflu.2021.105490 .

Pajnik, Jelena, Milovanović, Stoja, Stojanović, Dušica, Dimitrijević-Branković, Suzana, Janković-Častvan, Ivona, Uskoković, Petar, "Utilization of supercritical carbon dioxide for development of antibacterial surgical sutures" in Journal of Supercritical Fluids, 181 (2022):105490,
https://doi.org/10.1016/j.supflu.2021.105490 . .

TY  - CONF
AU  - Lukić, Ivana
AU  - Milovanović, Stoja
AU  - Stamenić, Marko
AU  - Tadić, Vanja
AU  - Skala, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6985
AB  - Cannabis sativa is one of the oldest plant cultures, which has been used for centuries as a source of textile fibres, for paper production, as well as a source of bioactive compounds used in medicine. Although being restrained for a long time, its high potential, coupled with the progress in legislation and/or legalization in some regions of the world, led to the significant increase of scientific research towards isolation of cannabinoids, primarily a non-psychotropic cannabidiol (CBD), over the past couple decades. 
Beside cannabinoids, found only in cannabis plants, Cannabis sativa is characterized by very complex chemical composition with almost 500 constituents, including terpenes, flavonoids, phenolic compounds and fatty acids [2]. The co-presence of these numerous molecules in preparations obtained from Cannabis sativa may lead to a greater biological activity due to  the “entourage” or synergistic effect [3]. CBD has attracted considerable interest due to its numerous therapeutic properties and pharmacological activities [1]. However, cannabinoids are characterized by a low solubility in aqueous media and, as a consequence, a relatively poor bioavailability. Therefore, improvement of their stability, shelf-life and bioavailability, by incorporation into biocompatible carriers is still a challenge. 
As an answer to this issue, integrated process of supercritical fluid extraction (SFE) from Cannabis sativa and supercritical solvent impregnation (SSI) of obtained extract into starch aerogel was tested. Starch gels were prepared starting from hydrogel formed from an aqueous solution of cornstarch (1:10 w/v). During replacement of water with acetone, by successive increase of its concentration until 100%, acetogel was formed and further subjected to drying using supercritical CO2 (scCO2) at 45 °C and 8-10 MPa to obtain aerogel. For comparison, a part of acetogel was dried in the oven at 60 °C to obtain xerogel. Textural properties and morphology of prepared materials were determined by N2 adsorption–desorption isotherms and scanning electron microscopy (SEM) analysis. Aerogel with highest specific surface area of 208.6 m2/g, obtained at 45 °C and 10 MPa, was further used for extract impregnation. Integrated SFE-SSI process was performed at 40 °C and 10 MPa, conditions determined as optimal in the process of SFE of Cannabis sativa taking into account the yield of the obtained extract based on the composition, primarily regarding the CBD content. The amounts of impregnated extracts were 2.6 and 15.3% for xerogel and aerogel, respectively. The presence of the extract on the surface of impregnated samples was confirmed by FTIR analysis. Release study showed controlled release of extracts in PBS solution.
C3  - 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022
T1  - Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation
SP  - P-28
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6985
ER  - 

@conference{
author = "Lukić, Ivana and Milovanović, Stoja and Stamenić, Marko and Tadić, Vanja and Skala, Dejan",
year = "2022",
abstract = "Cannabis sativa is one of the oldest plant cultures, which has been used for centuries as a source of textile fibres, for paper production, as well as a source of bioactive compounds used in medicine. Although being restrained for a long time, its high potential, coupled with the progress in legislation and/or legalization in some regions of the world, led to the significant increase of scientific research towards isolation of cannabinoids, primarily a non-psychotropic cannabidiol (CBD), over the past couple decades. 
Beside cannabinoids, found only in cannabis plants, Cannabis sativa is characterized by very complex chemical composition with almost 500 constituents, including terpenes, flavonoids, phenolic compounds and fatty acids [2]. The co-presence of these numerous molecules in preparations obtained from Cannabis sativa may lead to a greater biological activity due to  the “entourage” or synergistic effect [3]. CBD has attracted considerable interest due to its numerous therapeutic properties and pharmacological activities [1]. However, cannabinoids are characterized by a low solubility in aqueous media and, as a consequence, a relatively poor bioavailability. Therefore, improvement of their stability, shelf-life and bioavailability, by incorporation into biocompatible carriers is still a challenge. 
As an answer to this issue, integrated process of supercritical fluid extraction (SFE) from Cannabis sativa and supercritical solvent impregnation (SSI) of obtained extract into starch aerogel was tested. Starch gels were prepared starting from hydrogel formed from an aqueous solution of cornstarch (1:10 w/v). During replacement of water with acetone, by successive increase of its concentration until 100%, acetogel was formed and further subjected to drying using supercritical CO2 (scCO2) at 45 °C and 8-10 MPa to obtain aerogel. For comparison, a part of acetogel was dried in the oven at 60 °C to obtain xerogel. Textural properties and morphology of prepared materials were determined by N2 adsorption–desorption isotherms and scanning electron microscopy (SEM) analysis. Aerogel with highest specific surface area of 208.6 m2/g, obtained at 45 °C and 10 MPa, was further used for extract impregnation. Integrated SFE-SSI process was performed at 40 °C and 10 MPa, conditions determined as optimal in the process of SFE of Cannabis sativa taking into account the yield of the obtained extract based on the composition, primarily regarding the CBD content. The amounts of impregnated extracts were 2.6 and 15.3% for xerogel and aerogel, respectively. The presence of the extract on the surface of impregnated samples was confirmed by FTIR analysis. Release study showed controlled release of extracts in PBS solution.",
journal = "2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022",
title = "Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation",
pages = "P-28",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6985"
}

Lukić, I., Milovanović, S., Stamenić, M., Tadić, V.,& Skala, D.. (2022). Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation. in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022, P-28.
https://hdl.handle.net/21.15107/rcub_technorep_6985

Lukić I, Milovanović S, Stamenić M, Tadić V, Skala D. Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation. in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022. 2022;:P-28.
https://hdl.handle.net/21.15107/rcub_technorep_6985 .

Lukić, Ivana, Milovanović, Stoja, Stamenić, Marko, Tadić, Vanja, Skala, Dejan, "Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation" in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022 (2022):P-28,
https://hdl.handle.net/21.15107/rcub_technorep_6985 .

TY  - CONF
AU  - Lukić, Ivana
AU  - Pajnik, Jelena
AU  - Milovanović, Stoja
AU  - Tadić, Vanja
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6905
AB  - Bilberries (Vaccinium myrtillus) are believed to have one of the highest antioxidant levels of all
common fruits and vegetables. As a source of antioxidants, they have been used in traditional
medicine to reduce inflammation and protect against diseases associated with oxidative stress, such
as cardiovascular disease, diabetes, and age-related cognitive decline, as well as a remedy for eye
conditions. The biological activity of bilberries is associated with the high content of anthocyanins,
a large group of water-soluble flavonoids. Besides the complex anthocyanin profile, bilberries also
contain other phenolic compounds including flavonols, tannins, and phenolic acids. Supercritical fluid
extraction (SFE) isa a green and environmentally friendly process based on the use of supercritical
fluids, most commonly CO2, as an alternative to organic solvents, to obtain solvent-free and highly
valuable plant extracts. Utilization of supercritical CO2 (scCO2) extraction also enables the preservation
of thermally labile compounds working at low temperatures, and good selectivity accomplished by
the appropriate choice of operating pressure and temperature. In this study, scCO2 extraction was
investigated as a method for isolating the extract from bilberry dried fruit. Extractions were performed
at temperature of 70 °C and pressures of 15 and 30 MPa, with and without the ethanol as co-solvent,
with the aim to optimize the yield of the obtained extract based on the composition. The obtained
extraction yields ranged from 1.2 to 7% and the highest yield was obtained, as expected, at a higher
pressure of 30 MPa. The effect of ethanol as co-solvent in the scCO2 extraction was studied with the
goal to modify scCO2 selectivity and enhance the solubility of polar substances. The results showed a
positive effect on the extraction yield, while total phenolic content (TPC) analysis revealed that extracts
obtained using co-solvent have higher TPC values. Chemical analysis of the obtained extracts was
performed in order to qualitatively evaluate their composition.
PB  - Zagreb : University, Faculty of Food Technology and Biotechnology
C3  - Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022
T1  - SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT
SP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6905
ER  - 

@conference{
author = "Lukić, Ivana and Pajnik, Jelena and Milovanović, Stoja and Tadić, Vanja",
year = "2022",
abstract = "Bilberries (Vaccinium myrtillus) are believed to have one of the highest antioxidant levels of all
common fruits and vegetables. As a source of antioxidants, they have been used in traditional
medicine to reduce inflammation and protect against diseases associated with oxidative stress, such
as cardiovascular disease, diabetes, and age-related cognitive decline, as well as a remedy for eye
conditions. The biological activity of bilberries is associated with the high content of anthocyanins,
a large group of water-soluble flavonoids. Besides the complex anthocyanin profile, bilberries also
contain other phenolic compounds including flavonols, tannins, and phenolic acids. Supercritical fluid
extraction (SFE) isa a green and environmentally friendly process based on the use of supercritical
fluids, most commonly CO2, as an alternative to organic solvents, to obtain solvent-free and highly
valuable plant extracts. Utilization of supercritical CO2 (scCO2) extraction also enables the preservation
of thermally labile compounds working at low temperatures, and good selectivity accomplished by
the appropriate choice of operating pressure and temperature. In this study, scCO2 extraction was
investigated as a method for isolating the extract from bilberry dried fruit. Extractions were performed
at temperature of 70 °C and pressures of 15 and 30 MPa, with and without the ethanol as co-solvent,
with the aim to optimize the yield of the obtained extract based on the composition. The obtained
extraction yields ranged from 1.2 to 7% and the highest yield was obtained, as expected, at a higher
pressure of 30 MPa. The effect of ethanol as co-solvent in the scCO2 extraction was studied with the
goal to modify scCO2 selectivity and enhance the solubility of polar substances. The results showed a
positive effect on the extraction yield, while total phenolic content (TPC) analysis revealed that extracts
obtained using co-solvent have higher TPC values. Chemical analysis of the obtained extracts was
performed in order to qualitatively evaluate their composition.",
publisher = "Zagreb : University, Faculty of Food Technology and Biotechnology",
journal = "Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022",
title = "SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT",
pages = "100",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6905"
}

Lukić, I., Pajnik, J., Milovanović, S.,& Tadić, V.. (2022). SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT. in Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022
Zagreb : University, Faculty of Food Technology and Biotechnology., 100.
https://hdl.handle.net/21.15107/rcub_technorep_6905

Lukić I, Pajnik J, Milovanović S, Tadić V. SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT. in Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022. 2022;:100.
https://hdl.handle.net/21.15107/rcub_technorep_6905 .

Lukić, Ivana, Pajnik, Jelena, Milovanović, Stoja, Tadić, Vanja, "SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT" in Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022 (2022):100,
https://hdl.handle.net/21.15107/rcub_technorep_6905 .

TY  - CONF
AU  - Milovanović, Stoja
AU  - Dębczak, Agnieszka
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6904
AB  - Dandelion (Taraxacum officinale) is becoming an industry valuable crop due to the increase in its
utilization in the food and phytopharmaceutical industry. Nonetheless, its application is still limited
especially considering its extracts. Therefore, this study was aimed at exploring dandelion seeds as
a source of bioactive compounds. For this purpose, an environmentally friendly supercritical CO2
extraction technique was employed and extracts were separated at pressures of 10-45 MPa and a
temperature of 313 K. Obtained extracts were characterized using a gas chromatograph equipped
with mass spectroscopy (GC-MS), a gas chromatograph equipped with flame ionization detector (CGFID),
as well as by Folin–Ciocalteu and DPPH assays using a UV/VIS spectrophotometer. It was shown
that the selection of process pressure determined both extract yield and extract composition. An
increase in pressure increased extraction yield from 7.4 to 25.2% and the content of dominant linoleic
and oleic fatty acids (from 536.3 to 658.3 mg/g and 125.8 to 161.7 mg/g, respectively). Total phenolic
and flavonoid content in obtained extracts ranged from 5.5 to 9.0 mg GAE/g and from 208.6 to 497.5
mg QE/g, respectively. The strongest DPPH radical scavenging activity with inhibition of 64.4% was
recorded for extract obtained at 10 MPa for extract solution concentration of 20 mg/mL. Obtained
results confirmed that extracts obtained from dandelion seeds are a valuable source of bioactive
compounds.
PB  - Zagreb : University, Faculty of Food Technology and Biotechnology
C3  - Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022
T1  - SUPERCRITICAL CO2 EXTRACTION FROM DANDELION: THE EFFECT OF PRESSURE ON EXTRACTS YIELD AND COMPOSITION
SP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6904
ER  - 

@conference{
author = "Milovanović, Stoja and Dębczak, Agnieszka and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2022",
abstract = "Dandelion (Taraxacum officinale) is becoming an industry valuable crop due to the increase in its
utilization in the food and phytopharmaceutical industry. Nonetheless, its application is still limited
especially considering its extracts. Therefore, this study was aimed at exploring dandelion seeds as
a source of bioactive compounds. For this purpose, an environmentally friendly supercritical CO2
extraction technique was employed and extracts were separated at pressures of 10-45 MPa and a
temperature of 313 K. Obtained extracts were characterized using a gas chromatograph equipped
with mass spectroscopy (GC-MS), a gas chromatograph equipped with flame ionization detector (CGFID),
as well as by Folin–Ciocalteu and DPPH assays using a UV/VIS spectrophotometer. It was shown
that the selection of process pressure determined both extract yield and extract composition. An
increase in pressure increased extraction yield from 7.4 to 25.2% and the content of dominant linoleic
and oleic fatty acids (from 536.3 to 658.3 mg/g and 125.8 to 161.7 mg/g, respectively). Total phenolic
and flavonoid content in obtained extracts ranged from 5.5 to 9.0 mg GAE/g and from 208.6 to 497.5
mg QE/g, respectively. The strongest DPPH radical scavenging activity with inhibition of 64.4% was
recorded for extract obtained at 10 MPa for extract solution concentration of 20 mg/mL. Obtained
results confirmed that extracts obtained from dandelion seeds are a valuable source of bioactive
compounds.",
publisher = "Zagreb : University, Faculty of Food Technology and Biotechnology",
journal = "Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022",
title = "SUPERCRITICAL CO2 EXTRACTION FROM DANDELION: THE EFFECT OF PRESSURE ON EXTRACTS YIELD AND COMPOSITION",
pages = "101",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6904"
}

Milovanović, S., Dębczak, A., Tyśkiewicz, K.,& Konkol, M.. (2022). SUPERCRITICAL CO2 EXTRACTION FROM DANDELION: THE EFFECT OF PRESSURE ON EXTRACTS YIELD AND COMPOSITION. in Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022
Zagreb : University, Faculty of Food Technology and Biotechnology., 101.
https://hdl.handle.net/21.15107/rcub_technorep_6904

Milovanović S, Dębczak A, Tyśkiewicz K, Konkol M. SUPERCRITICAL CO2 EXTRACTION FROM DANDELION: THE EFFECT OF PRESSURE ON EXTRACTS YIELD AND COMPOSITION. in Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022. 2022;:101.
https://hdl.handle.net/21.15107/rcub_technorep_6904 .

Milovanović, Stoja, Dębczak, Agnieszka, Tyśkiewicz, Katarzyna, Konkol, Marcin, "SUPERCRITICAL CO2 EXTRACTION FROM DANDELION: THE EFFECT OF PRESSURE ON EXTRACTS YIELD AND COMPOSITION" in Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022 (2022):101,
https://hdl.handle.net/21.15107/rcub_technorep_6904 .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Pajnik, Jelena
AU  - Lukić, Ivana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4982
AB  - Poly(lactic acid) (PLA) stands out as the most promising biodegradable alternative to conventional petrochemical-based polymers for manufacturing of high-performance materials applied in medicine, pharmacy, food, textile, and electronic industry. This review was aimed to present the conventional and up-to-date technologies for PLA processing including melt blending and molding, hot melt extrusion, 3D printing, foaming, impregnation, thermally induced phase separation, nano- and microparticles preparation, wet and dry spinning processes. In addition, the effect of the processing parameters and polymer characteristics on the properties of the final material was elaborated. Diverse possibilities to tailor properties of PLA-based materials by variation in polymer characteristics and concentration, solvent selection, drying method, processing pressure and temperature, incorporation of bioactive components, and so on were highlighted. The examples of the relations between processing methods, parameters, and end-product properties are given for a better understanding of all aspects that need to be perceived for fabrication of PLA-based materials with required performances.
T2  - Journal of Applied Polymer Science
T1  - Tailoring of advanced poly(lactic acid)-based materials: A review
IS  - 12
VL  - 139
DO  - 10.1002/app.51839
ER  - 

@article{
author = "Milovanović, Stoja and Pajnik, Jelena and Lukić, Ivana",
year = "2022",
abstract = "Poly(lactic acid) (PLA) stands out as the most promising biodegradable alternative to conventional petrochemical-based polymers for manufacturing of high-performance materials applied in medicine, pharmacy, food, textile, and electronic industry. This review was aimed to present the conventional and up-to-date technologies for PLA processing including melt blending and molding, hot melt extrusion, 3D printing, foaming, impregnation, thermally induced phase separation, nano- and microparticles preparation, wet and dry spinning processes. In addition, the effect of the processing parameters and polymer characteristics on the properties of the final material was elaborated. Diverse possibilities to tailor properties of PLA-based materials by variation in polymer characteristics and concentration, solvent selection, drying method, processing pressure and temperature, incorporation of bioactive components, and so on were highlighted. The examples of the relations between processing methods, parameters, and end-product properties are given for a better understanding of all aspects that need to be perceived for fabrication of PLA-based materials with required performances.",
journal = "Journal of Applied Polymer Science",
title = "Tailoring of advanced poly(lactic acid)-based materials: A review",
number = "12",
volume = "139",
doi = "10.1002/app.51839"
}

Milovanović, S., Pajnik, J.,& Lukić, I.. (2022). Tailoring of advanced poly(lactic acid)-based materials: A review. in Journal of Applied Polymer Science, 139(12).
https://doi.org/10.1002/app.51839

Milovanović S, Pajnik J, Lukić I. Tailoring of advanced poly(lactic acid)-based materials: A review. in Journal of Applied Polymer Science. 2022;139(12).
doi:10.1002/app.51839 .

Milovanović, Stoja, Pajnik, Jelena, Lukić, Ivana, "Tailoring of advanced poly(lactic acid)-based materials: A review" in Journal of Applied Polymer Science, 139, no. 12 (2022),
https://doi.org/10.1002/app.51839 . .

TY  - JOUR
AU  - Barać, Nemanja
AU  - Barcelo, Ernest
AU  - Stojanović, Dušica
AU  - Milovanović, Stoja
AU  - Uskoković, Petar
AU  - Gane, Patrick
AU  - Dimic-Misic, Katarina
AU  - Imani, Monireh
AU  - Janaćković, Đorđe
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5007
AB  - In this work, we examine two modifications of fine-ground calcium carbonate material (GCC) in order to enhanced sorption of NO2 and subsequent reaction properties toward NO2-/NO3- formation by firstly exposing the GCC to supercritical (sc) CO2 in order to increase particle surface area, a choice specifically made to avoid altering the surface chemistry, and secondly considering the potential advantage of using a surface coupling agent toward NO2. The modification by the coupling agent amino silane (AMEO silane) was applied in a supercritical CO2-ethanol mixture. The samples were characterised before and after modification by field emission scanning electron microscopy (FESEM), specific surface area determination (BET nitrogen adsorption), ATR-FTIR spectroscopy and ion chromatography to reveal the effects of the surface modification(s) on the morphology, surface textural properties and sorption versus reaction properties with NO2. The performance of the treated sorbents for NO2 capture was evaluated at room temperature. Results show that reactivity of NO2 with GCC was observed to increase as a function of increased surface area resulting from scCO(2) exposure, but that the presence of AMEO silane on the surface, while enhancing initial adsorption of NO2 was seen subsequently to act to block reactivity. Thus, judicious use of coupling agent can provide desired rapid initial adsorption of the gas, but the goal of long-term CaCO3-consuming reactivity, so as to prolong the uptake of NO2 beyond surface saturation alone, is achieved by increasing surface area while retaining chemical-free exposed CaCO3 surface.
T2  - Environmental Science and Pollution Research
T1  - Modification of CaCO3 and CaCO3 pin-coated cellulose paper under supercritical carbon dioxide-ethanol mixture for enhanced NO2 capture
EP  - 11717
IS  - 8
SP  - 11707
VL  - 29
DO  - 10.1007/s11356-021-16503-9
ER  - 

@article{
author = "Barać, Nemanja and Barcelo, Ernest and Stojanović, Dušica and Milovanović, Stoja and Uskoković, Petar and Gane, Patrick and Dimic-Misic, Katarina and Imani, Monireh and Janaćković, Đorđe",
year = "2022",
abstract = "In this work, we examine two modifications of fine-ground calcium carbonate material (GCC) in order to enhanced sorption of NO2 and subsequent reaction properties toward NO2-/NO3- formation by firstly exposing the GCC to supercritical (sc) CO2 in order to increase particle surface area, a choice specifically made to avoid altering the surface chemistry, and secondly considering the potential advantage of using a surface coupling agent toward NO2. The modification by the coupling agent amino silane (AMEO silane) was applied in a supercritical CO2-ethanol mixture. The samples were characterised before and after modification by field emission scanning electron microscopy (FESEM), specific surface area determination (BET nitrogen adsorption), ATR-FTIR spectroscopy and ion chromatography to reveal the effects of the surface modification(s) on the morphology, surface textural properties and sorption versus reaction properties with NO2. The performance of the treated sorbents for NO2 capture was evaluated at room temperature. Results show that reactivity of NO2 with GCC was observed to increase as a function of increased surface area resulting from scCO(2) exposure, but that the presence of AMEO silane on the surface, while enhancing initial adsorption of NO2 was seen subsequently to act to block reactivity. Thus, judicious use of coupling agent can provide desired rapid initial adsorption of the gas, but the goal of long-term CaCO3-consuming reactivity, so as to prolong the uptake of NO2 beyond surface saturation alone, is achieved by increasing surface area while retaining chemical-free exposed CaCO3 surface.",
journal = "Environmental Science and Pollution Research",
title = "Modification of CaCO3 and CaCO3 pin-coated cellulose paper under supercritical carbon dioxide-ethanol mixture for enhanced NO2 capture",
pages = "11717-11707",
number = "8",
volume = "29",
doi = "10.1007/s11356-021-16503-9"
}

Barać, N., Barcelo, E., Stojanović, D., Milovanović, S., Uskoković, P., Gane, P., Dimic-Misic, K., Imani, M.,& Janaćković, Đ.. (2022). Modification of CaCO3 and CaCO3 pin-coated cellulose paper under supercritical carbon dioxide-ethanol mixture for enhanced NO2 capture. in Environmental Science and Pollution Research, 29(8), 11707-11717.
https://doi.org/10.1007/s11356-021-16503-9

Barać N, Barcelo E, Stojanović D, Milovanović S, Uskoković P, Gane P, Dimic-Misic K, Imani M, Janaćković Đ. Modification of CaCO3 and CaCO3 pin-coated cellulose paper under supercritical carbon dioxide-ethanol mixture for enhanced NO2 capture. in Environmental Science and Pollution Research. 2022;29(8):11707-11717.
doi:10.1007/s11356-021-16503-9 .

Barać, Nemanja, Barcelo, Ernest, Stojanović, Dušica, Milovanović, Stoja, Uskoković, Petar, Gane, Patrick, Dimic-Misic, Katarina, Imani, Monireh, Janaćković, Đorđe, "Modification of CaCO3 and CaCO3 pin-coated cellulose paper under supercritical carbon dioxide-ethanol mixture for enhanced NO2 capture" in Environmental Science and Pollution Research, 29, no. 8 (2022):11707-11717,
https://doi.org/10.1007/s11356-021-16503-9 . .

TY  - JOUR
AU  - Lukić, Ivana
AU  - Milovanović, Stoja
AU  - Pantić, Milica
AU  - Srbljak, Ivana
AU  - Djurić, Ana
AU  - Tadić, Vanja
AU  - Tyśkiewicz, Katarzyna
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5093
AB  - Extracts from Silybum marianum seeds have high industrial potential due to their application as phytopharmaceuticals and food. In order to optimize its production, conventional extraction (in Soxhlet apparatus using solvents ethanol and n-hexane) and supercritical fluid extraction (using CO2 at the pressure of 30 MPa and temperature of 40 °C with and without co-solvent ethanol, from oil-rich and defatted seeds) were compared. In addition, the effect of plant cultivation (years 2011 and 2019) and the effect of seeds and extract storage on the yield and quality of extracts were assessed. It was shown that unsaturated fatty acids (linoleic and oleic acids being most dominant) constituted 64–87% of obtained extracts. Content of α-tocopherol varied from 0.01 to 13 g/100 g, while total phenolic content was in the range from 13.2 to 104.2 gGAE/100 gextract. In vitro cytotoxic activity analysis confirmed that extracts obtained from defatted seeds showed activity against cancer cells. This study pointed out the important role of the cultivation year, selection of extraction technique and solvent, as well as storage on the extraction yield, chemical profile, and cytotoxic activity of extracts.
PB  - Academic Press
T2  - LWT
T1  - Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity
SP  - 113319
VL  - 160
DO  - 10.1016/j.lwt.2022.113319
ER  - 

@article{
author = "Lukić, Ivana and Milovanović, Stoja and Pantić, Milica and Srbljak, Ivana and Djurić, Ana and Tadić, Vanja and Tyśkiewicz, Katarzyna",
year = "2022",
abstract = "Extracts from Silybum marianum seeds have high industrial potential due to their application as phytopharmaceuticals and food. In order to optimize its production, conventional extraction (in Soxhlet apparatus using solvents ethanol and n-hexane) and supercritical fluid extraction (using CO2 at the pressure of 30 MPa and temperature of 40 °C with and without co-solvent ethanol, from oil-rich and defatted seeds) were compared. In addition, the effect of plant cultivation (years 2011 and 2019) and the effect of seeds and extract storage on the yield and quality of extracts were assessed. It was shown that unsaturated fatty acids (linoleic and oleic acids being most dominant) constituted 64–87% of obtained extracts. Content of α-tocopherol varied from 0.01 to 13 g/100 g, while total phenolic content was in the range from 13.2 to 104.2 gGAE/100 gextract. In vitro cytotoxic activity analysis confirmed that extracts obtained from defatted seeds showed activity against cancer cells. This study pointed out the important role of the cultivation year, selection of extraction technique and solvent, as well as storage on the extraction yield, chemical profile, and cytotoxic activity of extracts.",
publisher = "Academic Press",
journal = "LWT",
title = "Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity",
pages = "113319",
volume = "160",
doi = "10.1016/j.lwt.2022.113319"
}

Lukić, I., Milovanović, S., Pantić, M., Srbljak, I., Djurić, A., Tadić, V.,& Tyśkiewicz, K.. (2022). Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity. in LWT
Academic Press., 160, 113319.
https://doi.org/10.1016/j.lwt.2022.113319

Lukić I, Milovanović S, Pantić M, Srbljak I, Djurić A, Tadić V, Tyśkiewicz K. Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity. in LWT. 2022;160:113319.
doi:10.1016/j.lwt.2022.113319 .

Lukić, Ivana, Milovanović, Stoja, Pantić, Milica, Srbljak, Ivana, Djurić, Ana, Tadić, Vanja, Tyśkiewicz, Katarzyna, "Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity" in LWT, 160 (2022):113319,
https://doi.org/10.1016/j.lwt.2022.113319 . .