TY  - JOUR
AU  - Milovanović, Stoja
AU  - Marković, Darka
AU  - Janković-Častvan, Ivona
AU  - Lukić, Ivana
PY  - 2024-05
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7258
AB  - This study focuses on the development of bioactive materials using environmentally friendly techniques, renewable, biocompatible, and biodegradable polysaccharide, as well as natural bioactive compounds (NBCs) found in plant extracts. First, cornstarch aerogels with a porosity of 86 % and a specific surface area of 225 m2/g were produced via supercritical CO2- assisted drying. Further, thymol, citronellol, carvacrol, and eugenol were incorporated into the aerogels by supercritical CO2- assisted impregnation, which allowed variation in loadings of NBCs (12.8–17.6 %). Interaction between cornstarch aerogels and NBCs determined impregnation rate, pore wall thickness (in the range 18–95 nm), liquid absorption capacity (from 265 to 569 %), dehydration mass loss, and release in phosphate-buffered saline. Controlled release of NBCs was maintained over a 3-day period. Moreover, impregnated aerogels showed a significant antioxidant effect with the highest value for DPPH radical inhibition of 25.5 % determined for the aerogels impregnated with eugenol. Notable antimicrobial activity against tested Gram-negative bacteria, Gram-positive bacteria, and fungi was also observed, being the highest for thymol-loaded aerogel with the diameter of the inhibition zones of up to 37.5 mm. This work shows a promising green approach for the production of bioactive two-component starch-based materials for potential applications in skin infection treatment.
PB  - Elsevier Ltd.
T2  - Carbohydrate Polymers
T1  - Cornstarch aerogels with thymol, citronellol, carvacrol, and eugenol prepared by supercritical CO2- assisted techniques for potential biomedical applications
SP  - 121874
VL  - 331
DO  - 10.1016/j.carbpol.2024.121874
ER  - 

@article{
author = "Milovanović, Stoja and Marković, Darka and Janković-Častvan, Ivona and Lukić, Ivana",
year = "2024-05",
abstract = "This study focuses on the development of bioactive materials using environmentally friendly techniques, renewable, biocompatible, and biodegradable polysaccharide, as well as natural bioactive compounds (NBCs) found in plant extracts. First, cornstarch aerogels with a porosity of 86 % and a specific surface area of 225 m2/g were produced via supercritical CO2- assisted drying. Further, thymol, citronellol, carvacrol, and eugenol were incorporated into the aerogels by supercritical CO2- assisted impregnation, which allowed variation in loadings of NBCs (12.8–17.6 %). Interaction between cornstarch aerogels and NBCs determined impregnation rate, pore wall thickness (in the range 18–95 nm), liquid absorption capacity (from 265 to 569 %), dehydration mass loss, and release in phosphate-buffered saline. Controlled release of NBCs was maintained over a 3-day period. Moreover, impregnated aerogels showed a significant antioxidant effect with the highest value for DPPH radical inhibition of 25.5 % determined for the aerogels impregnated with eugenol. Notable antimicrobial activity against tested Gram-negative bacteria, Gram-positive bacteria, and fungi was also observed, being the highest for thymol-loaded aerogel with the diameter of the inhibition zones of up to 37.5 mm. This work shows a promising green approach for the production of bioactive two-component starch-based materials for potential applications in skin infection treatment.",
publisher = "Elsevier Ltd.",
journal = "Carbohydrate Polymers",
title = "Cornstarch aerogels with thymol, citronellol, carvacrol, and eugenol prepared by supercritical CO2- assisted techniques for potential biomedical applications",
pages = "121874",
volume = "331",
doi = "10.1016/j.carbpol.2024.121874"
}

Milovanović, S., Marković, D., Janković-Častvan, I.,& Lukić, I.. (2024-05). Cornstarch aerogels with thymol, citronellol, carvacrol, and eugenol prepared by supercritical CO2- assisted techniques for potential biomedical applications. in Carbohydrate Polymers
Elsevier Ltd.., 331, 121874.
https://doi.org/10.1016/j.carbpol.2024.121874

Milovanović S, Marković D, Janković-Častvan I, Lukić I. Cornstarch aerogels with thymol, citronellol, carvacrol, and eugenol prepared by supercritical CO2- assisted techniques for potential biomedical applications. in Carbohydrate Polymers. 2024;331:121874.
doi:10.1016/j.carbpol.2024.121874 .

Milovanović, Stoja, Marković, Darka, Janković-Častvan, Ivona, Lukić, Ivana, "Cornstarch aerogels with thymol, citronellol, carvacrol, and eugenol prepared by supercritical CO2- assisted techniques for potential biomedical applications" in Carbohydrate Polymers, 331 (2024-05):121874,
https://doi.org/10.1016/j.carbpol.2024.121874 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Lukić, Ivana
AU  - Krgović, Nemanja
AU  - Tadić, Vanja
AU  - Radovanović, Željko
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2024-04
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7140
AB  - The study aimed toward the development of biocompatible and biodegradable polymeric films (starch-chitosan and starch-chitosan-cyclodextrin) with biologically valuable green tea leaf extract using an environmentally friendly integrated process of supercritical fluid extraction (SFE) and supercritical solvent impregnation (SSI). The SFE-SSI process parameters were selected through the initial estimation of the SFE parameters effect (temperatures 35 and 70 °C and the addition of co-solvents ethanol, ethanol/water, and water). The present early-stage work showed that an increase in temperature increased extraction yield (from 0.9 to 4.2%) and caffeine content while it decreased polyphenolic and pigment content as well as antioxidant activity and extract loading onto films (from 7.1 to 0.1%). The addition of co-solvents to sc-CO2 had an adverse effect on extract yield, composition, and loading. The highest recorded total phenolic, flavonoid, chlorophyll A, and caffeine content in extracts were 136.5 mg GE/g, 22.7 mg RE/g, 8.1 mg/g, and 460.0 mg/g, respectively.
PB  - Elsevier B.V.
T2  - Journal of Supercritical Fluids
T1  - Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films
SP  - 106163
VL  - 206
DO  - 10.1016/j.supflu.2023.106163
ER  - 

@article{
author = "Milovanović, Stoja and Lukić, Ivana and Krgović, Nemanja and Tadić, Vanja and Radovanović, Željko and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2024-04",
abstract = "The study aimed toward the development of biocompatible and biodegradable polymeric films (starch-chitosan and starch-chitosan-cyclodextrin) with biologically valuable green tea leaf extract using an environmentally friendly integrated process of supercritical fluid extraction (SFE) and supercritical solvent impregnation (SSI). The SFE-SSI process parameters were selected through the initial estimation of the SFE parameters effect (temperatures 35 and 70 °C and the addition of co-solvents ethanol, ethanol/water, and water). The present early-stage work showed that an increase in temperature increased extraction yield (from 0.9 to 4.2%) and caffeine content while it decreased polyphenolic and pigment content as well as antioxidant activity and extract loading onto films (from 7.1 to 0.1%). The addition of co-solvents to sc-CO2 had an adverse effect on extract yield, composition, and loading. The highest recorded total phenolic, flavonoid, chlorophyll A, and caffeine content in extracts were 136.5 mg GE/g, 22.7 mg RE/g, 8.1 mg/g, and 460.0 mg/g, respectively.",
publisher = "Elsevier B.V.",
journal = "Journal of Supercritical Fluids",
title = "Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films",
pages = "106163",
volume = "206",
doi = "10.1016/j.supflu.2023.106163"
}

Milovanović, S., Lukić, I., Krgović, N., Tadić, V., Radovanović, Ž., Tyśkiewicz, K.,& Konkol, M.. (2024-04). Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films. in Journal of Supercritical Fluids
Elsevier B.V.., 206, 106163.
https://doi.org/10.1016/j.supflu.2023.106163

Milovanović S, Lukić I, Krgović N, Tadić V, Radovanović Ž, Tyśkiewicz K, Konkol M. Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films. in Journal of Supercritical Fluids. 2024;206:106163.
doi:10.1016/j.supflu.2023.106163 .

Milovanović, Stoja, Lukić, Ivana, Krgović, Nemanja, Tadić, Vanja, Radovanović, Željko, Tyśkiewicz, Katarzyna, Konkol, Marcin, "Selection of processing parameters for the integrated supercritical CO2 extraction from green tea leaves and extract impregnation onto starch-chitosan based films" in Journal of Supercritical Fluids, 206 (2024-04):106163,
https://doi.org/10.1016/j.supflu.2023.106163 . .

TY  - CONF
AU  - Lukić, Ivana
AU  - Milovanović, Stoja
AU  - Krgović, Nemanja
AU  - Tadić, Vanja
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6907
AB  - Green tea is considered to be a plant with a variety of health benefits due to its antioxidative, antimicrobial,
anti-inflammatory, and anticancer activity. Besides the most important bioactive constituents, including
polyphenols, especially catechins, and flavonoids, green tea is rich in caffeine, and such complex composition
makes its extract suitable as a component of active food packaging or skin care systems [1,2]. The
technologies commonly proposed to obtain such materials involve some limitations, mainly related to the use
of liquid organic solvents that lead to low penetration of the active compound into the polymeric platform and,
consequently, limited loading efficiencies, non-unique dispersion, and solvent residue in the final material. An
excellent technique for direct loading of natural bioactive compounds from plant materials into polymeric
carriers that can overcome the aforementioned challenges is an integrated process accomplished by merging
supercritical fluid extraction (SFE) and supercritical solvent impregnation (SSI). Therefore, in this study, the
preparation of two types of polymer films and their functionalization with green tea leaf extract using the SFESSI
process was tested.
Starch/chitosan (SC) films were prepared by mixing the 1% chitosan solution with the 1% starch solution in a
mass ratio of 1:1, while starch/chitosan/β-cyclodextrin (SCCD) films were prepared by addition of 1% β-
cyclodextrin to starch and chitosan to obtain the ratio of 1:1:1. After solvent casting and drying at room
temperature, the films were additionally dried in an oven overnight at 35 °C. Initially, supercritical CO2
(scCO2) extraction was investigated as a method for isolating the extract from green tea at temperatures of 35
and 70 °C and pressure of 30 MPa, with and without the ethanol as a co-solvent. The obtained extraction yields
ranged from 0.91 to 4.22%, being the highest at a higher temperature. The chemical composition of the
obtained extracts was determined, as well as the total phenolic content (TPC), total flavonoid content (TFC),
total chlorophyll, and total carotenoid content. HPLC analysis revealed that caffeine was the major constituent
of all extracts with the amount ranging from 24.1 to 202.9 mg/g. The IC50 values determined using the DPPH
assay showed that the extract obtained at 35 °C with the addition of ethanol exhibited the strongest antioxidant
activity. After determining the SFE conditions that gave the green tea extract with high content of bioactive
compounds, prepared films were impregnated using the integrated SFE-SSI process for 4 h at the same
conditions as scCO2 extraction. The loading of green tea extracts onto films was up to 7.7% showing that lower
temperature (35 °C) and the addition of ethanol had a favourable influence. The presence of the extract on the
surface of impregnated samples was confirmed by FTIR analysis.
PB  - Budapest : University of Technology and Economics
C3  - Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
T1  - Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films
SP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6907
ER  - 

@conference{
author = "Lukić, Ivana and Milovanović, Stoja and Krgović, Nemanja and Tadić, Vanja and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2023",
abstract = "Green tea is considered to be a plant with a variety of health benefits due to its antioxidative, antimicrobial,
anti-inflammatory, and anticancer activity. Besides the most important bioactive constituents, including
polyphenols, especially catechins, and flavonoids, green tea is rich in caffeine, and such complex composition
makes its extract suitable as a component of active food packaging or skin care systems [1,2]. The
technologies commonly proposed to obtain such materials involve some limitations, mainly related to the use
of liquid organic solvents that lead to low penetration of the active compound into the polymeric platform and,
consequently, limited loading efficiencies, non-unique dispersion, and solvent residue in the final material. An
excellent technique for direct loading of natural bioactive compounds from plant materials into polymeric
carriers that can overcome the aforementioned challenges is an integrated process accomplished by merging
supercritical fluid extraction (SFE) and supercritical solvent impregnation (SSI). Therefore, in this study, the
preparation of two types of polymer films and their functionalization with green tea leaf extract using the SFESSI
process was tested.
Starch/chitosan (SC) films were prepared by mixing the 1% chitosan solution with the 1% starch solution in a
mass ratio of 1:1, while starch/chitosan/β-cyclodextrin (SCCD) films were prepared by addition of 1% β-
cyclodextrin to starch and chitosan to obtain the ratio of 1:1:1. After solvent casting and drying at room
temperature, the films were additionally dried in an oven overnight at 35 °C. Initially, supercritical CO2
(scCO2) extraction was investigated as a method for isolating the extract from green tea at temperatures of 35
and 70 °C and pressure of 30 MPa, with and without the ethanol as a co-solvent. The obtained extraction yields
ranged from 0.91 to 4.22%, being the highest at a higher temperature. The chemical composition of the
obtained extracts was determined, as well as the total phenolic content (TPC), total flavonoid content (TFC),
total chlorophyll, and total carotenoid content. HPLC analysis revealed that caffeine was the major constituent
of all extracts with the amount ranging from 24.1 to 202.9 mg/g. The IC50 values determined using the DPPH
assay showed that the extract obtained at 35 °C with the addition of ethanol exhibited the strongest antioxidant
activity. After determining the SFE conditions that gave the green tea extract with high content of bioactive
compounds, prepared films were impregnated using the integrated SFE-SSI process for 4 h at the same
conditions as scCO2 extraction. The loading of green tea extracts onto films was up to 7.7% showing that lower
temperature (35 °C) and the addition of ethanol had a favourable influence. The presence of the extract on the
surface of impregnated samples was confirmed by FTIR analysis.",
publisher = "Budapest : University of Technology and Economics",
journal = "Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023",
title = "Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films",
pages = "124",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6907"
}

Lukić, I., Milovanović, S., Krgović, N., Tadić, V., Tyśkiewicz, K.,& Konkol, M.. (2023). Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
Budapest : University of Technology and Economics., 124.
https://hdl.handle.net/21.15107/rcub_technorep_6907

Lukić I, Milovanović S, Krgović N, Tadić V, Tyśkiewicz K, Konkol M. Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023. 2023;:124.
https://hdl.handle.net/21.15107/rcub_technorep_6907 .

Lukić, Ivana, Milovanović, Stoja, Krgović, Nemanja, Tadić, Vanja, Tyśkiewicz, Katarzyna, Konkol, Marcin, "Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films" in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 (2023):124,
https://hdl.handle.net/21.15107/rcub_technorep_6907 .

TY  - CONF
AU  - Lukić, Ivana
AU  - Stamenić, Marko
AU  - Tadić, Vanja
AU  - Skala, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6984
AB  - The high potential of industrial hemp (Cannabis sativa), mainly due to the high-quality fibers and bioactive compounds, coupled with the decriminalization and/or legalization in some regions of the world, led to the steady growth of scientific research over the past couple of decades [1, 2]. From an industrial point of view, cannabinoids, primarily non-psychotropic cannabidiol (CBD), are considered the most valuable compounds, as they possess a broad range of therapeutic properties, such as anxiolytic, neuroprotective, antibiotic, antiinflammatory activity, and anti-oxidant. The hemp industry has already adopted supercritical carbon dioxide scCO2 extraction as a well-established process since it is a promising ‘green’ alternative to conventional extraction methods, selective, with short processing time, and low impact on the environment.
In the present study, the preliminary analysis was performed to determine the influence of different
scCO2 densities based on the different combinations of process pressure and temperature on the kinetics of supercritical extraction of Cannabis sativa, extract yield and the chemical composition of obtained extracts. Experiments were performed at temperatures ranging from 40 to 60 °C and pressures ranging from 10 to 30 MPa. Prior to extraction, the plant material was grounded and submitted to decarboxylation process at 120 °C for 1 h, in order to transform cannabidiolic acid, which is the precursor present in the plant material, to CBD.
Experimental design for optimization plays an important role both in science and industry, because it allows evaluation of the effects of multiple factors and their interactions on one or more response variables. The present work was focused on the further optimization of scCO2 extraction with the aim to maximize the yield of extracts with high recoveries of CBD, by means of response surface methodology (RSM), based on a central composite design (CCD) using Design of Experiment software (Stat-Ease, Design Expert). For this purpose, independent variables were temperature (40-60 °C), pressure (10-30 MPa), and scCO2 consumption (20-60 gCO2/gplant).
The results showed that supercritical CO2 extraction enabled the achievement of up to 5% of total extract yield and 266–435 mg/g of CBD depending on the conditions. The high pressure and temperature, 30 MPa 60 °C, respectively, conditions that correspond to the highest density of the scCO2, generated the highest extract yield. On the contrary, lower pressure, corresponding to the lower density of CO2 was favourable for achieving a high CBD content. In addition, a higher scCO2 consumption, which is related to longer processing time, was the most dominant factor affecting the extraction process exerting a significant effect, on both examined responses, yield and CBD content. A linear model equation was proposed to express both the yield and the CBD content as a function of independent variables.
PB  - Budapest : University of Technology and Economics
C3  - Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
T1  - Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach
SP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6984
ER  - 

@conference{
author = "Lukić, Ivana and Stamenić, Marko and Tadić, Vanja and Skala, Dejan",
year = "2023",
abstract = "The high potential of industrial hemp (Cannabis sativa), mainly due to the high-quality fibers and bioactive compounds, coupled with the decriminalization and/or legalization in some regions of the world, led to the steady growth of scientific research over the past couple of decades [1, 2]. From an industrial point of view, cannabinoids, primarily non-psychotropic cannabidiol (CBD), are considered the most valuable compounds, as they possess a broad range of therapeutic properties, such as anxiolytic, neuroprotective, antibiotic, antiinflammatory activity, and anti-oxidant. The hemp industry has already adopted supercritical carbon dioxide scCO2 extraction as a well-established process since it is a promising ‘green’ alternative to conventional extraction methods, selective, with short processing time, and low impact on the environment.
In the present study, the preliminary analysis was performed to determine the influence of different
scCO2 densities based on the different combinations of process pressure and temperature on the kinetics of supercritical extraction of Cannabis sativa, extract yield and the chemical composition of obtained extracts. Experiments were performed at temperatures ranging from 40 to 60 °C and pressures ranging from 10 to 30 MPa. Prior to extraction, the plant material was grounded and submitted to decarboxylation process at 120 °C for 1 h, in order to transform cannabidiolic acid, which is the precursor present in the plant material, to CBD.
Experimental design for optimization plays an important role both in science and industry, because it allows evaluation of the effects of multiple factors and their interactions on one or more response variables. The present work was focused on the further optimization of scCO2 extraction with the aim to maximize the yield of extracts with high recoveries of CBD, by means of response surface methodology (RSM), based on a central composite design (CCD) using Design of Experiment software (Stat-Ease, Design Expert). For this purpose, independent variables were temperature (40-60 °C), pressure (10-30 MPa), and scCO2 consumption (20-60 gCO2/gplant).
The results showed that supercritical CO2 extraction enabled the achievement of up to 5% of total extract yield and 266–435 mg/g of CBD depending on the conditions. The high pressure and temperature, 30 MPa 60 °C, respectively, conditions that correspond to the highest density of the scCO2, generated the highest extract yield. On the contrary, lower pressure, corresponding to the lower density of CO2 was favourable for achieving a high CBD content. In addition, a higher scCO2 consumption, which is related to longer processing time, was the most dominant factor affecting the extraction process exerting a significant effect, on both examined responses, yield and CBD content. A linear model equation was proposed to express both the yield and the CBD content as a function of independent variables.",
publisher = "Budapest : University of Technology and Economics",
journal = "Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023",
title = "Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach",
pages = "135",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6984"
}

Lukić, I., Stamenić, M., Tadić, V.,& Skala, D.. (2023). Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
Budapest : University of Technology and Economics., 135.
https://hdl.handle.net/21.15107/rcub_technorep_6984

Lukić I, Stamenić M, Tadić V, Skala D. Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023. 2023;:135.
https://hdl.handle.net/21.15107/rcub_technorep_6984 .

Lukić, Ivana, Stamenić, Marko, Tadić, Vanja, Skala, Dejan, "Optimization of Cannabis sativa supercritical CO2 extraction using Design of experiments approach" in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 (2023):135,
https://hdl.handle.net/21.15107/rcub_technorep_6984 .

TY  - CONF
AU  - Mladenović, Mihailo
AU  - Grbić, Jovana
AU  - Moskovljević, Mia
AU  - Bogdanović, Mihajlo
AU  - Mladenović, Dragana
AU  - Lukić, Ivana
AU  - Đukić-Vuković, Aleksandra
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6944
AB  - During the processing of herbs, a significant amount of chopped biomass by-products is left behind, often discarded and underutilized. Since they are important sources of different groups of bioactives like polyphenolics, essential oils or terpenes and structural compounds like lignocellulose, they could be exploited in fermentation by different microorganisms. However, fractionation and extractions could significantly increase the effectiveness and productivity of the valorization process. We examined the possibility of extracting antioxidants from by-products of hoary willowherb (Epilobium spp.), sage (Salvia spp.), and
basil (Ocimum spp.), as medicinal herbs with significant pharmaceutical and market value. Extracts of hoary willowherb, sage and basil residues were obtained by a conventional, Soxhlet extraction for polar compounds and supercritical carbon(IV)-oxide extraction as an unconventional extraction method for non-polar compounds. We measured the antioxidant content in the obtained extracts by the ABTS and DPPH spectrophotometric methods. In addition, we measured the total polyphenol content of the extracts by the Folin-Coicalteu
method. According to the ABTS method, the antioxidant activity in the sage extracts obtained from Soxhlet extraction is 2.2 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity in the basil extracts obtained from Soxhlet extraction is 9.1 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction, according to the DPPH method. The total polyphenol content in the hoary willowherb extracts obtained from Soxhlet extraction is 4.6 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity is greater in the extracts obtained by Soxhlet extraction than in the extracts obtained with supercritical carbon(IV)-oxide extraction. Because we examined residues from the tea processing industry, the content of easily volatile non-polar components is expected to be lower. The results confirm that valorization of these residues should be focused on the polar components and their microbial biotransformations.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - By-products from the processing of herbs as sources of antioxidants
SP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6944
ER  - 

@conference{
author = "Mladenović, Mihailo and Grbić, Jovana and Moskovljević, Mia and Bogdanović, Mihajlo and Mladenović, Dragana and Lukić, Ivana and Đukić-Vuković, Aleksandra",
year = "2023",
abstract = "During the processing of herbs, a significant amount of chopped biomass by-products is left behind, often discarded and underutilized. Since they are important sources of different groups of bioactives like polyphenolics, essential oils or terpenes and structural compounds like lignocellulose, they could be exploited in fermentation by different microorganisms. However, fractionation and extractions could significantly increase the effectiveness and productivity of the valorization process. We examined the possibility of extracting antioxidants from by-products of hoary willowherb (Epilobium spp.), sage (Salvia spp.), and
basil (Ocimum spp.), as medicinal herbs with significant pharmaceutical and market value. Extracts of hoary willowherb, sage and basil residues were obtained by a conventional, Soxhlet extraction for polar compounds and supercritical carbon(IV)-oxide extraction as an unconventional extraction method for non-polar compounds. We measured the antioxidant content in the obtained extracts by the ABTS and DPPH spectrophotometric methods. In addition, we measured the total polyphenol content of the extracts by the Folin-Coicalteu
method. According to the ABTS method, the antioxidant activity in the sage extracts obtained from Soxhlet extraction is 2.2 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity in the basil extracts obtained from Soxhlet extraction is 9.1 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction, according to the DPPH method. The total polyphenol content in the hoary willowherb extracts obtained from Soxhlet extraction is 4.6 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity is greater in the extracts obtained by Soxhlet extraction than in the extracts obtained with supercritical carbon(IV)-oxide extraction. Because we examined residues from the tea processing industry, the content of easily volatile non-polar components is expected to be lower. The results confirm that valorization of these residues should be focused on the polar components and their microbial biotransformations.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "By-products from the processing of herbs as sources of antioxidants",
pages = "20",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6944"
}

Mladenović, M., Grbić, J., Moskovljević, M., Bogdanović, M., Mladenović, D., Lukić, I.,& Đukić-Vuković, A.. (2023). By-products from the processing of herbs as sources of antioxidants. in Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 20.
https://hdl.handle.net/21.15107/rcub_technorep_6944

Mladenović M, Grbić J, Moskovljević M, Bogdanović M, Mladenović D, Lukić I, Đukić-Vuković A. By-products from the processing of herbs as sources of antioxidants. in Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:20.
https://hdl.handle.net/21.15107/rcub_technorep_6944 .

Mladenović, Mihailo, Grbić, Jovana, Moskovljević, Mia, Bogdanović, Mihajlo, Mladenović, Dragana, Lukić, Ivana, Đukić-Vuković, Aleksandra, "By-products from the processing of herbs as sources of antioxidants" in Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):20,
https://hdl.handle.net/21.15107/rcub_technorep_6944 .

TY  - CONF
AU  - Lukić, Ivana
AU  - Kesić, Željka
AU  - Zdujić, Miodrag
AU  - Skala, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6934
AB  - Biodiesel, a mixture of fatty acid methyl esters (FAME), is an excellent non-toxic, and biodegradable substitute for mineral diesel fuels produced from crude oil. It is made from renewable sources by transesterification of triglycerides with methanol, or by a reaction usually defined as methanolysis. Usage of low-quality feedstock, such as waste cooking oil (WCO), is challenging due to the undesirable side reactions as a result of the presence of free fatty acids (FFA), and water, thus a pretreatment stage before subjecting it to the transesterification process is usually required. In the present study, a two-step approach based on an adsorptive pretreatment with quicklime in order to remove FFA from WCO, followed by methanolysis using CaO∙ZnO as a heterogeneous catalyst was proposed. The first step was analyzed with the goal to define the optimal temperature of adsorption, the adsorbent particle size, calcination procedure, as well as the necessary amount of quicklime used. The analysis of adsorption kinetics was performed using pseudo-first and pseudo-second order kinetic models, and the efficiency of quicklime in the FFA removal from the WCO was also determined. The results showed that FFA from WCO could be successfully removed using quicklime as adsorbent at 30 °C for 1 h, with a removal efficiency of 72% and an adsorption capacity of 910 mg/g. The amount of Ca2+ ion present in the oil after the pretreatment was determined to be 12.64 mg/kg, showing that a very small amount of calcium from CaO was dissolved in treated WCO. The FTIR analysis of quicklime after the adsorption of FFA confirmed the interaction of the carboxyl group in FFA with the active site of the adsorbent. Adsorptive pretreatment had a positive effect on the rate of transesterification reaction with CaO∙ZnO as a catalyst, enabling the achievement of over 96% of FAME yield in only 15 min at 60 °C. The present study showed that quicklime, being cheap, available, and efficient, has considerable potential for the removal of FFA from the WCO. Besides, both the adsorption and the heterogeneously catalyzed methanolysis are environmentally and economically acceptable processes.
PB  - Valladolid : The lnstitute of Bioeconomy of University of Valladolid
C3  - Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain
T1  - Adsorptive pretreatment of waste cooking oil using quicklime for two-step biodiesel production
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6934
ER  - 

@conference{
author = "Lukić, Ivana and Kesić, Željka and Zdujić, Miodrag and Skala, Dejan",
year = "2023",
abstract = "Biodiesel, a mixture of fatty acid methyl esters (FAME), is an excellent non-toxic, and biodegradable substitute for mineral diesel fuels produced from crude oil. It is made from renewable sources by transesterification of triglycerides with methanol, or by a reaction usually defined as methanolysis. Usage of low-quality feedstock, such as waste cooking oil (WCO), is challenging due to the undesirable side reactions as a result of the presence of free fatty acids (FFA), and water, thus a pretreatment stage before subjecting it to the transesterification process is usually required. In the present study, a two-step approach based on an adsorptive pretreatment with quicklime in order to remove FFA from WCO, followed by methanolysis using CaO∙ZnO as a heterogeneous catalyst was proposed. The first step was analyzed with the goal to define the optimal temperature of adsorption, the adsorbent particle size, calcination procedure, as well as the necessary amount of quicklime used. The analysis of adsorption kinetics was performed using pseudo-first and pseudo-second order kinetic models, and the efficiency of quicklime in the FFA removal from the WCO was also determined. The results showed that FFA from WCO could be successfully removed using quicklime as adsorbent at 30 °C for 1 h, with a removal efficiency of 72% and an adsorption capacity of 910 mg/g. The amount of Ca2+ ion present in the oil after the pretreatment was determined to be 12.64 mg/kg, showing that a very small amount of calcium from CaO was dissolved in treated WCO. The FTIR analysis of quicklime after the adsorption of FFA confirmed the interaction of the carboxyl group in FFA with the active site of the adsorbent. Adsorptive pretreatment had a positive effect on the rate of transesterification reaction with CaO∙ZnO as a catalyst, enabling the achievement of over 96% of FAME yield in only 15 min at 60 °C. The present study showed that quicklime, being cheap, available, and efficient, has considerable potential for the removal of FFA from the WCO. Besides, both the adsorption and the heterogeneously catalyzed methanolysis are environmentally and economically acceptable processes.",
publisher = "Valladolid : The lnstitute of Bioeconomy of University of Valladolid",
journal = "Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain",
title = "Adsorptive pretreatment of waste cooking oil using quicklime for two-step biodiesel production",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6934"
}

Lukić, I., Kesić, Ž., Zdujić, M.,& Skala, D.. (2023). Adsorptive pretreatment of waste cooking oil using quicklime for two-step biodiesel production. in Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain
Valladolid : The lnstitute of Bioeconomy of University of Valladolid..
https://hdl.handle.net/21.15107/rcub_technorep_6934

Lukić I, Kesić Ž, Zdujić M, Skala D. Adsorptive pretreatment of waste cooking oil using quicklime for two-step biodiesel production. in Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6934 .

Lukić, Ivana, Kesić, Željka, Zdujić, Miodrag, Skala, Dejan, "Adsorptive pretreatment of waste cooking oil using quicklime for two-step biodiesel production" in Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6934 .

TY  - PAT
AU  - Tadić, Vanja
AU  - Žugić, Ana
AU  - Nešić, Ivana
AU  - Krgović, Nemanja
AU  - Mudrić, Jelena
AU  - Martinović, Milica
AU  - Stojanović, Sanja
AU  - Maksimović, Svetolik
AU  - Lukić, Ivana
AU  - Petrović, Slobodan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6924
T2  - Zavod za intelektualnu svojinu republike Srbije
T1  - Gastroretentivni fitopreparat sa sistemskim delovanjem za sniženje nivoa šećera u krvi i postupak dobijanja
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6924
ER  - 

@misc{
author = "Tadić, Vanja and Žugić, Ana and Nešić, Ivana and Krgović, Nemanja and Mudrić, Jelena and Martinović, Milica and Stojanović, Sanja and Maksimović, Svetolik and Lukić, Ivana and Petrović, Slobodan",
year = "2023",
journal = "Zavod za intelektualnu svojinu republike Srbije",
title = "Gastroretentivni fitopreparat sa sistemskim delovanjem za sniženje nivoa šećera u krvi i postupak dobijanja",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6924"
}

Tadić, V., Žugić, A., Nešić, I., Krgović, N., Mudrić, J., Martinović, M., Stojanović, S., Maksimović, S., Lukić, I.,& Petrović, S.. (2023). Gastroretentivni fitopreparat sa sistemskim delovanjem za sniženje nivoa šećera u krvi i postupak dobijanja. in Zavod za intelektualnu svojinu republike Srbije.
https://hdl.handle.net/21.15107/rcub_technorep_6924

Tadić V, Žugić A, Nešić I, Krgović N, Mudrić J, Martinović M, Stojanović S, Maksimović S, Lukić I, Petrović S. Gastroretentivni fitopreparat sa sistemskim delovanjem za sniženje nivoa šećera u krvi i postupak dobijanja. in Zavod za intelektualnu svojinu republike Srbije. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6924 .

Tadić, Vanja, Žugić, Ana, Nešić, Ivana, Krgović, Nemanja, Mudrić, Jelena, Martinović, Milica, Stojanović, Sanja, Maksimović, Svetolik, Lukić, Ivana, Petrović, Slobodan, "Gastroretentivni fitopreparat sa sistemskim delovanjem za sniženje nivoa šećera u krvi i postupak dobijanja" in Zavod za intelektualnu svojinu republike Srbije (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6924 .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Lukić, Ivana
AU  - Horvat, Gabrijela
AU  - Novak, Zoran
AU  - Frerich, Sulamith
AU  - Petermann, Marcus
AU  - García-González, Carlos A.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6032
AB  - This review provides a concise overview of up-to-date developments in the processing of neat poly(lactic acid) (PLA), improvement in its properties, and preparation of advanced materials using a green medium (CO2 under elevated pressure). Pressurized CO2 in the dense and supercritical state is a superior alternative medium to organic solvents, as it is easily available, fully recyclable, has easily tunable properties, and can be completely removed from the final material without post-processing steps. This review summarizes the state of the art on PLA drying, impregnation, foaming, and particle generation by the employment of dense and supercritical CO2 for the development of new materials. An analysis of the effect of processing methods on the final material properties was focused on neat PLA and PLA with an addition of natural bioactive components. It was demonstrated that CO2-assisted processes enable the control of PLA properties, reduce operating times, and require less energy compared to conventional ones. The described environmentally friendly processing techniques and the versatility of PLA were employed for the preparation of foams, aerogels, scaffolds, microparticles, and nanoparticles, as well as bioactive materials. These PLA-based materials can find application in tissue engineering, drug delivery, active food packaging, compostable packaging, wastewater treatment, or thermal insulation, among others.
PB  - MDPI
T2  - Polymers
T1  - Green Processing of Neat Poly(lactic acid) Using Carbon Dioxide under Elevated Pressure for Preparation of Advanced Materials: A Review (2012–2022)
IS  - 4
SP  - 860
VL  - 15
DO  - 10.3390/polym15040860
ER  - 

@article{
author = "Milovanović, Stoja and Lukić, Ivana and Horvat, Gabrijela and Novak, Zoran and Frerich, Sulamith and Petermann, Marcus and García-González, Carlos A.",
year = "2023",
abstract = "This review provides a concise overview of up-to-date developments in the processing of neat poly(lactic acid) (PLA), improvement in its properties, and preparation of advanced materials using a green medium (CO2 under elevated pressure). Pressurized CO2 in the dense and supercritical state is a superior alternative medium to organic solvents, as it is easily available, fully recyclable, has easily tunable properties, and can be completely removed from the final material without post-processing steps. This review summarizes the state of the art on PLA drying, impregnation, foaming, and particle generation by the employment of dense and supercritical CO2 for the development of new materials. An analysis of the effect of processing methods on the final material properties was focused on neat PLA and PLA with an addition of natural bioactive components. It was demonstrated that CO2-assisted processes enable the control of PLA properties, reduce operating times, and require less energy compared to conventional ones. The described environmentally friendly processing techniques and the versatility of PLA were employed for the preparation of foams, aerogels, scaffolds, microparticles, and nanoparticles, as well as bioactive materials. These PLA-based materials can find application in tissue engineering, drug delivery, active food packaging, compostable packaging, wastewater treatment, or thermal insulation, among others.",
publisher = "MDPI",
journal = "Polymers",
title = "Green Processing of Neat Poly(lactic acid) Using Carbon Dioxide under Elevated Pressure for Preparation of Advanced Materials: A Review (2012–2022)",
number = "4",
pages = "860",
volume = "15",
doi = "10.3390/polym15040860"
}

Milovanović, S., Lukić, I., Horvat, G., Novak, Z., Frerich, S., Petermann, M.,& García-González, C. A.. (2023). Green Processing of Neat Poly(lactic acid) Using Carbon Dioxide under Elevated Pressure for Preparation of Advanced Materials: A Review (2012–2022). in Polymers
MDPI., 15(4), 860.
https://doi.org/10.3390/polym15040860

Milovanović S, Lukić I, Horvat G, Novak Z, Frerich S, Petermann M, García-González CA. Green Processing of Neat Poly(lactic acid) Using Carbon Dioxide under Elevated Pressure for Preparation of Advanced Materials: A Review (2012–2022). in Polymers. 2023;15(4):860.
doi:10.3390/polym15040860 .

Milovanović, Stoja, Lukić, Ivana, Horvat, Gabrijela, Novak, Zoran, Frerich, Sulamith, Petermann, Marcus, García-González, Carlos A., "Green Processing of Neat Poly(lactic acid) Using Carbon Dioxide under Elevated Pressure for Preparation of Advanced Materials: A Review (2012–2022)" in Polymers, 15, no. 4 (2023):860,
https://doi.org/10.3390/polym15040860 . .

TY  - CONF
AU  - Lukić, Ivana
AU  - Milovanović, Stoja
AU  - Tadić, Vanja
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6791
AB  - Bilberries (Vaccinium myrtillus) have been used in traditional medicine to reduce
inflammation and protect against diseases associated with oxidative stress, such as
cardiovascular disease, diabetes, and age-related cognitive decline, as well as a
remedy for eye conditions. The biological activity of bilberries is ascribed mainly to
their high content of anthocyanins, but also to other valuable phytochemicals,
including flavonols, phenolic acids, and tannins [1,2]. The enhanced stability and
bioavailability of bioactive components from plant material could be accomplished
by their incorporation into a polymeric carrier, using the promising integrated
processes of supercritical fluid extraction (SFE) and supercritical solvent
impregnation (SSI). ...
PB  - Maribor : University of Maribor
PB  - Maribor : University Press
C3  - Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia
T1  - Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel
EP  - 109
SP  - 107
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6791
ER  - 

@conference{
author = "Lukić, Ivana and Milovanović, Stoja and Tadić, Vanja",
year = "2023",
abstract = "Bilberries (Vaccinium myrtillus) have been used in traditional medicine to reduce
inflammation and protect against diseases associated with oxidative stress, such as
cardiovascular disease, diabetes, and age-related cognitive decline, as well as a
remedy for eye conditions. The biological activity of bilberries is ascribed mainly to
their high content of anthocyanins, but also to other valuable phytochemicals,
including flavonols, phenolic acids, and tannins [1,2]. The enhanced stability and
bioavailability of bioactive components from plant material could be accomplished
by their incorporation into a polymeric carrier, using the promising integrated
processes of supercritical fluid extraction (SFE) and supercritical solvent
impregnation (SSI). ...",
publisher = "Maribor : University of Maribor, Maribor : University Press",
journal = "Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia",
title = "Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel",
pages = "109-107",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6791"
}

Lukić, I., Milovanović, S.,& Tadić, V.. (2023). Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel. in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia
Maribor : University of Maribor., 107-109.
https://hdl.handle.net/21.15107/rcub_technorep_6791

Lukić I, Milovanović S, Tadić V. Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel. in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia. 2023;:107-109.
https://hdl.handle.net/21.15107/rcub_technorep_6791 .

Lukić, Ivana, Milovanović, Stoja, Tadić, Vanja, "Supercritical CO2 Extraction from Bilberry (Vaccinium myrtillus) Fruit and Impregnation of the Obtained Extract Onto Starch Aerogel" in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia (2023):107-109,
https://hdl.handle.net/21.15107/rcub_technorep_6791 .

TY  - CONF
AU  - Milovanović, Stoja
AU  - Lukić, Ivana
AU  - Marković, Darka
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6792
AB  - Natural compounds found in different plant extracts can show numerous biological
activities and be used as health promoters. For instance, thymol, carvacrol,
citronellol, and eugenol (found in extracts of thyme, oregano, lemongrass, and clove,
respectively) can be used as anti-inflammatory, antiviral, and antimicrobial agents [1-
4]. The high volatility of the mentioned natural bioactive compounds (NBCs), which
restricts their broader application, can be managed by the incorporation of NBCs
into polymer matrices. Therefore, intending to develop devices that release
antimicrobial substances in a controlled manner, this study tested the applicability
of the supercritical solvent impregnation (SSI) technique for the incorporation of
NBCs into starch aerogels. First, the hydrogels were prepared from corn starch. The
water in the hydrogels was replaced by ethanol, and the obtained alcogels were dried
using the supercritical drying (SCD) technique. The parameters of the aerogels`
preparation were optimized previously [5].Later, SSI was performed at 150 bar and
35 °C for 2 h. The obtained materials were analyzed using FTIR, to determine the
potential interactions between the NBCs and polymer that could determine the rate
of controlled release and antimicrobial activity. Controlled release of NBCs from the
aerogels was tested in phosphate-buffered saline solution at 37 °C. ...
PB  - Maribor : University of Maribor
PB  - Maribor : University Press
C3  - Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia
T1  - Supercritical Solvent Impregnation Technique for the Development of Antimicrobial Starch-based Aerogels
EP  - 165
SP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6792
ER  - 

@conference{
author = "Milovanović, Stoja and Lukić, Ivana and Marković, Darka",
year = "2023",
abstract = "Natural compounds found in different plant extracts can show numerous biological
activities and be used as health promoters. For instance, thymol, carvacrol,
citronellol, and eugenol (found in extracts of thyme, oregano, lemongrass, and clove,
respectively) can be used as anti-inflammatory, antiviral, and antimicrobial agents [1-
4]. The high volatility of the mentioned natural bioactive compounds (NBCs), which
restricts their broader application, can be managed by the incorporation of NBCs
into polymer matrices. Therefore, intending to develop devices that release
antimicrobial substances in a controlled manner, this study tested the applicability
of the supercritical solvent impregnation (SSI) technique for the incorporation of
NBCs into starch aerogels. First, the hydrogels were prepared from corn starch. The
water in the hydrogels was replaced by ethanol, and the obtained alcogels were dried
using the supercritical drying (SCD) technique. The parameters of the aerogels`
preparation were optimized previously [5].Later, SSI was performed at 150 bar and
35 °C for 2 h. The obtained materials were analyzed using FTIR, to determine the
potential interactions between the NBCs and polymer that could determine the rate
of controlled release and antimicrobial activity. Controlled release of NBCs from the
aerogels was tested in phosphate-buffered saline solution at 37 °C. ...",
publisher = "Maribor : University of Maribor, Maribor : University Press",
journal = "Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia",
title = "Supercritical Solvent Impregnation Technique for the Development of Antimicrobial Starch-based Aerogels",
pages = "165-163",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6792"
}

Milovanović, S., Lukić, I.,& Marković, D.. (2023). Supercritical Solvent Impregnation Technique for the Development of Antimicrobial Starch-based Aerogels. in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia
Maribor : University of Maribor., 163-165.
https://hdl.handle.net/21.15107/rcub_technorep_6792

Milovanović S, Lukić I, Marković D. Supercritical Solvent Impregnation Technique for the Development of Antimicrobial Starch-based Aerogels. in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia. 2023;:163-165.
https://hdl.handle.net/21.15107/rcub_technorep_6792 .

Milovanović, Stoja, Lukić, Ivana, Marković, Darka, "Supercritical Solvent Impregnation Technique for the Development of Antimicrobial Starch-based Aerogels" in Book of Abstracts / 3rd International Conference on Aerogels for Biomedical and Environmental Applications, 5–7 July 2023, Maribor, Slovenia (2023):163-165,
https://hdl.handle.net/21.15107/rcub_technorep_6792 .

TY  - JOUR
AU  - Lukić, Ivana
AU  - Kesić, Željka
AU  - Zdujić, Miodrag
AU  - Skala, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6569
AB  - Synthesis of biodiesel from various plant oils is realized by the transesterification of triglycerides with methanol or by a reaction usually defined as methanolysis. The usage of low-quality oils, such as waste cooking oil (WCO), is followed by undesirable side reactions as a result of the increased content of free fatty acids (FFA), and water. The presence of FFA in WCO usually requires a pretreatment stage before subjecting it to methanolysis. In the present work, heterogeneously catalyzed methanolysis of WCO with and without pretreatment was investigated. Removal of FFA from WCO was conducted by using only quicklime or with the addition of a small amount of methanol (FFA to methanol = 1:3 molar ratio). The obtained results showed that pretreatment of WCO with quicklime at 30 °C after 1 h reduces the FFA content by 72 %, while the adsorption capacity was determined to be 910 mg g-1. The adsorptive pretreatment, as a simple operation, using low-cost quicklime under mild conditions, had a positive effect on the transesterification rate with CaO∙ZnO as a catalyst, enabling the achievement of over 96 % of biodiesel yield in only 15 min, compared to 1 h without the pretreatment. Furthermore, pretreated WCO allows an increase in repeated catalyst use and overall savings in the necessary amount of catalyst. The present study showed that quicklime is an economic, environmental-friendly, and sustainable material for FFA removal from WCO.
PB  - Association of the Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Adsorptive pretreatment of waste cooking oil using quicklime for fatty acid methyl esters synthesis
EP  - 84
IS  - 1
SP  - 69
VL  - 77
DO  - 10.2298/HEMIND220628005L
ER  - 

@article{
author = "Lukić, Ivana and Kesić, Željka and Zdujić, Miodrag and Skala, Dejan",
year = "2023",
abstract = "Synthesis of biodiesel from various plant oils is realized by the transesterification of triglycerides with methanol or by a reaction usually defined as methanolysis. The usage of low-quality oils, such as waste cooking oil (WCO), is followed by undesirable side reactions as a result of the increased content of free fatty acids (FFA), and water. The presence of FFA in WCO usually requires a pretreatment stage before subjecting it to methanolysis. In the present work, heterogeneously catalyzed methanolysis of WCO with and without pretreatment was investigated. Removal of FFA from WCO was conducted by using only quicklime or with the addition of a small amount of methanol (FFA to methanol = 1:3 molar ratio). The obtained results showed that pretreatment of WCO with quicklime at 30 °C after 1 h reduces the FFA content by 72 %, while the adsorption capacity was determined to be 910 mg g-1. The adsorptive pretreatment, as a simple operation, using low-cost quicklime under mild conditions, had a positive effect on the transesterification rate with CaO∙ZnO as a catalyst, enabling the achievement of over 96 % of biodiesel yield in only 15 min, compared to 1 h without the pretreatment. Furthermore, pretreated WCO allows an increase in repeated catalyst use and overall savings in the necessary amount of catalyst. The present study showed that quicklime is an economic, environmental-friendly, and sustainable material for FFA removal from WCO.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Adsorptive pretreatment of waste cooking oil using quicklime for fatty acid methyl esters synthesis",
pages = "84-69",
number = "1",
volume = "77",
doi = "10.2298/HEMIND220628005L"
}

Lukić, I., Kesić, Ž., Zdujić, M.,& Skala, D.. (2023). Adsorptive pretreatment of waste cooking oil using quicklime for fatty acid methyl esters synthesis. in Hemijska industrija
Association of the Chemical Engineers of Serbia., 77(1), 69-84.
https://doi.org/10.2298/HEMIND220628005L

Lukić I, Kesić Ž, Zdujić M, Skala D. Adsorptive pretreatment of waste cooking oil using quicklime for fatty acid methyl esters synthesis. in Hemijska industrija. 2023;77(1):69-84.
doi:10.2298/HEMIND220628005L .

Lukić, Ivana, Kesić, Željka, Zdujić, Miodrag, Skala, Dejan, "Adsorptive pretreatment of waste cooking oil using quicklime for fatty acid methyl esters synthesis" in Hemijska industrija, 77, no. 1 (2023):69-84,
https://doi.org/10.2298/HEMIND220628005L . .

TY  - JOUR
AU  - Pajnik, Jelena
AU  - Dikić, Jelena
AU  - Milovanović, Stoja
AU  - Milošević, Milena
AU  - Jevtić, Sanja
AU  - Lukić, Ivana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5085
AB  - Chitosan/gelatin and chitosan/gelatin/zeolite films prepared by solvent casting method were
impregnated with a mixture of thymol and carvacrol using a green solvent, supercritical carbon 
dioxide at 35 °C and 30 MPa, during 18 h. Proposed method enabled preparation of biocompatible 
and biodegradable blends with strong antioxidant and antibacterial activity, whereby amounts of 
loaded thymol/carvacrol mixture were in the range from 3.3-6%. After initial burst release, both 
types of films exhibited gradual release of bioactive compounds, with around 72 and 96% of 
impregnated thymol/carvacrol mixture released in water and PBS (pH 7.4) during tested period of 10 days, respectively. Results of water vapor transmission rate (> 76 gm-2
day-1) confirmed that prepared composites are suitable for wound dressing application. Thermal analysis showed superior properties of prepared thymol/carvacrol loaded films compared to control samples. In addition, mechanical and structural properties, as well as solubility and swelling behavior of the obtained films were investigated in detail.
PB  - Wiley
T2  - Macromolecular Materials and Engineering
T1  - Zeolite/chitosan/gelatin films: preparation, supercritical CO2 processing, characterization and bioactivity
EP  - 13
IS  - 4
SP  - 1
VL  - 307
DO  - 10.1002/mame.202200009
ER  - 

@article{
author = "Pajnik, Jelena and Dikić, Jelena and Milovanović, Stoja and Milošević, Milena and Jevtić, Sanja and Lukić, Ivana",
year = "2022",
abstract = "Chitosan/gelatin and chitosan/gelatin/zeolite films prepared by solvent casting method were
impregnated with a mixture of thymol and carvacrol using a green solvent, supercritical carbon 
dioxide at 35 °C and 30 MPa, during 18 h. Proposed method enabled preparation of biocompatible 
and biodegradable blends with strong antioxidant and antibacterial activity, whereby amounts of 
loaded thymol/carvacrol mixture were in the range from 3.3-6%. After initial burst release, both 
types of films exhibited gradual release of bioactive compounds, with around 72 and 96% of 
impregnated thymol/carvacrol mixture released in water and PBS (pH 7.4) during tested period of 10 days, respectively. Results of water vapor transmission rate (> 76 gm-2
day-1) confirmed that prepared composites are suitable for wound dressing application. Thermal analysis showed superior properties of prepared thymol/carvacrol loaded films compared to control samples. In addition, mechanical and structural properties, as well as solubility and swelling behavior of the obtained films were investigated in detail.",
publisher = "Wiley",
journal = "Macromolecular Materials and Engineering",
title = "Zeolite/chitosan/gelatin films: preparation, supercritical CO2 processing, characterization and bioactivity",
pages = "13-1",
number = "4",
volume = "307",
doi = "10.1002/mame.202200009"
}

Pajnik, J., Dikić, J., Milovanović, S., Milošević, M., Jevtić, S.,& Lukić, I.. (2022). Zeolite/chitosan/gelatin films: preparation, supercritical CO2 processing, characterization and bioactivity. in Macromolecular Materials and Engineering
Wiley., 307(4), 1-13.
https://doi.org/10.1002/mame.202200009

Pajnik J, Dikić J, Milovanović S, Milošević M, Jevtić S, Lukić I. Zeolite/chitosan/gelatin films: preparation, supercritical CO2 processing, characterization and bioactivity. in Macromolecular Materials and Engineering. 2022;307(4):1-13.
doi:10.1002/mame.202200009 .

Pajnik, Jelena, Dikić, Jelena, Milovanović, Stoja, Milošević, Milena, Jevtić, Sanja, Lukić, Ivana, "Zeolite/chitosan/gelatin films: preparation, supercritical CO2 processing, characterization and bioactivity" in Macromolecular Materials and Engineering, 307, no. 4 (2022):1-13,
https://doi.org/10.1002/mame.202200009 . .

TY  - JOUR
AU  - Pajnik, Jelena
AU  - Dikić, Jelena
AU  - Milovanović, Stoja
AU  - Milošević, Milena
AU  - Jevtić, Sanja
AU  - Lukić, Ivana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5086
AB  - Chitosan/gelatin and chitosan/gelatin/zeolite films prepared by solvent casting method were impregnated with a mixture of thymol and carvacrol using a green solvent, supercritical carbon dioxide at 35 °C and 30 MPa, during 18 h. Proposed method enabled preparation of biocompatible and biodegradable blends with strong antioxidant and antibacterial activity, whereby amounts of loaded thymol/carvacrol mixture were in the range from 3.3-6%. After initial burst release, both types of films exhibited gradual release of bioactive compounds, with around 72 and 96% of impregnated thymol/carvacrol mixture released in water and PBS (pH 7.4) during tested period of 10 days, respectively. Results of water vapor transmission rate (> 76 gm-2 day-1) confirmed that prepared composites are suitable for wound dressing application. Thermal analysis showed superior properties of prepared thymol/carvacrol loaded films compared to control samples. In addition, mechanical and structural properties, as well as solubility and swelling behavior of the obtained films were investigated in detail.
PB  - Wiley
T2  - Macromolecular Materials and Engineering
T1  - Zeolite/Chitosan/Gelatin Films: Preparation, SupercriticalCO2Processing, Characterization, and Bioactivity
EP  - 13
IS  - 4
SP  - 1
VL  - 307
DO  - 10.1002/mame.202200009
ER  - 

@article{
author = "Pajnik, Jelena and Dikić, Jelena and Milovanović, Stoja and Milošević, Milena and Jevtić, Sanja and Lukić, Ivana",
year = "2022",
abstract = "Chitosan/gelatin and chitosan/gelatin/zeolite films prepared by solvent casting method were impregnated with a mixture of thymol and carvacrol using a green solvent, supercritical carbon dioxide at 35 °C and 30 MPa, during 18 h. Proposed method enabled preparation of biocompatible and biodegradable blends with strong antioxidant and antibacterial activity, whereby amounts of loaded thymol/carvacrol mixture were in the range from 3.3-6%. After initial burst release, both types of films exhibited gradual release of bioactive compounds, with around 72 and 96% of impregnated thymol/carvacrol mixture released in water and PBS (pH 7.4) during tested period of 10 days, respectively. Results of water vapor transmission rate (> 76 gm-2 day-1) confirmed that prepared composites are suitable for wound dressing application. Thermal analysis showed superior properties of prepared thymol/carvacrol loaded films compared to control samples. In addition, mechanical and structural properties, as well as solubility and swelling behavior of the obtained films were investigated in detail.",
publisher = "Wiley",
journal = "Macromolecular Materials and Engineering",
title = "Zeolite/Chitosan/Gelatin Films: Preparation, SupercriticalCO2Processing, Characterization, and Bioactivity",
pages = "13-1",
number = "4",
volume = "307",
doi = "10.1002/mame.202200009"
}

Pajnik, J., Dikić, J., Milovanović, S., Milošević, M., Jevtić, S.,& Lukić, I.. (2022). Zeolite/Chitosan/Gelatin Films: Preparation, SupercriticalCO2Processing, Characterization, and Bioactivity. in Macromolecular Materials and Engineering
Wiley., 307(4), 1-13.
https://doi.org/10.1002/mame.202200009

Pajnik J, Dikić J, Milovanović S, Milošević M, Jevtić S, Lukić I. Zeolite/Chitosan/Gelatin Films: Preparation, SupercriticalCO2Processing, Characterization, and Bioactivity. in Macromolecular Materials and Engineering. 2022;307(4):1-13.
doi:10.1002/mame.202200009 .

Pajnik, Jelena, Dikić, Jelena, Milovanović, Stoja, Milošević, Milena, Jevtić, Sanja, Lukić, Ivana, "Zeolite/Chitosan/Gelatin Films: Preparation, SupercriticalCO2Processing, Characterization, and Bioactivity" in Macromolecular Materials and Engineering, 307, no. 4 (2022):1-13,
https://doi.org/10.1002/mame.202200009 . .

TY  - CONF
AU  - Stefanović, Milica
AU  - Lukić, Ivana
AU  - Vujančević, Jelena
AU  - Petrović, Rada
AU  - Janaćković, Đorđe
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6170
AB  - Supercritical carbon dioxide (sCO2) is an ideal low-temperature cosolvent for perovskite
deposition due to its relatively low critical point (31.2 °C, 73.8 bar), no surface tension, liquidlike density, gas-like viscosity, and diffusivity. It enables faster mass transfer which allows
penetration of crystals in nanoporous structure. The study investigates the influence of time of
deposition of perovskite assisted with supercritical carbon dioxide on the filling of nanotubes.
Perovskite solar cell technology has been developed so fast due to several factors including a
tunable band gap, high absorption coefficient, and low-cost fabrication. The quality of the
perovskite film is important for the high efficiency of perovskite solar cells. Perovskite precursors
are usually deposited from the solution onto a substrate using spin-coating followed by postdeposition treatments, but often it results in low-quality films that cannot provide good
photovoltaic performances. Deposition of perovskite in the presence of sCO2 is a promising
method for the formation of high-quality perovskite layers. In this work, methylammonium lead
bromide perovskite (MAPbBr3) was deposited on TiO2 nanotubes from the solution in
dimethylformamide (DMF) by application of sCO2 at 35 °C and 200 bar for 1 h, and 3 h. FESEM
results show that TiO2 nanotubes were filled with perovskite material in both cases. The diffuse
reflectance spectroscopy measurement of samples proved that the absorption edge of prepared
TiO2 nanotubes/MAPbBr3 was extended to the visible range. Measurement of I-V characteristics
showed that the sample made for 3 h had a higher value of current than the sample prepared for 1
h. The application of sCO2 during the deposition of perovskite has enabled the preparation of a
photodiode with a better contact between TiO2 nanotubes and perovskite which is important for
the future development of solar cells.
PB  - Materials Research Society of Serbia
C3  - 23th Annual Conference YUCOMAT 2022 and 12th WRTCS
T1  - Supercritical CO2 assisted deposition of MAPbBr3 perovskite onto TiO2 nanotubes
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6170
ER  - 

@conference{
author = "Stefanović, Milica and Lukić, Ivana and Vujančević, Jelena and Petrović, Rada and Janaćković, Đorđe",
year = "2022",
abstract = "Supercritical carbon dioxide (sCO2) is an ideal low-temperature cosolvent for perovskite
deposition due to its relatively low critical point (31.2 °C, 73.8 bar), no surface tension, liquidlike density, gas-like viscosity, and diffusivity. It enables faster mass transfer which allows
penetration of crystals in nanoporous structure. The study investigates the influence of time of
deposition of perovskite assisted with supercritical carbon dioxide on the filling of nanotubes.
Perovskite solar cell technology has been developed so fast due to several factors including a
tunable band gap, high absorption coefficient, and low-cost fabrication. The quality of the
perovskite film is important for the high efficiency of perovskite solar cells. Perovskite precursors
are usually deposited from the solution onto a substrate using spin-coating followed by postdeposition treatments, but often it results in low-quality films that cannot provide good
photovoltaic performances. Deposition of perovskite in the presence of sCO2 is a promising
method for the formation of high-quality perovskite layers. In this work, methylammonium lead
bromide perovskite (MAPbBr3) was deposited on TiO2 nanotubes from the solution in
dimethylformamide (DMF) by application of sCO2 at 35 °C and 200 bar for 1 h, and 3 h. FESEM
results show that TiO2 nanotubes were filled with perovskite material in both cases. The diffuse
reflectance spectroscopy measurement of samples proved that the absorption edge of prepared
TiO2 nanotubes/MAPbBr3 was extended to the visible range. Measurement of I-V characteristics
showed that the sample made for 3 h had a higher value of current than the sample prepared for 1
h. The application of sCO2 during the deposition of perovskite has enabled the preparation of a
photodiode with a better contact between TiO2 nanotubes and perovskite which is important for
the future development of solar cells.",
publisher = "Materials Research Society of Serbia",
journal = "23th Annual Conference YUCOMAT 2022 and 12th WRTCS",
title = "Supercritical CO2 assisted deposition of MAPbBr3 perovskite onto TiO2 nanotubes",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6170"
}

Stefanović, M., Lukić, I., Vujančević, J., Petrović, R.,& Janaćković, Đ.. (2022). Supercritical CO2 assisted deposition of MAPbBr3 perovskite onto TiO2 nanotubes. in 23th Annual Conference YUCOMAT 2022 and 12th WRTCS
Materials Research Society of Serbia., 73.
https://hdl.handle.net/21.15107/rcub_technorep_6170

Stefanović M, Lukić I, Vujančević J, Petrović R, Janaćković Đ. Supercritical CO2 assisted deposition of MAPbBr3 perovskite onto TiO2 nanotubes. in 23th Annual Conference YUCOMAT 2022 and 12th WRTCS. 2022;:73.
https://hdl.handle.net/21.15107/rcub_technorep_6170 .

Stefanović, Milica, Lukić, Ivana, Vujančević, Jelena, Petrović, Rada, Janaćković, Đorđe, "Supercritical CO2 assisted deposition of MAPbBr3 perovskite onto TiO2 nanotubes" in 23th Annual Conference YUCOMAT 2022 and 12th WRTCS (2022):73,
https://hdl.handle.net/21.15107/rcub_technorep_6170 .

TY  - CONF
AU  - Stefanović, Milica
AU  - Lukić, Ivana
AU  - Vujančević, Jelena
AU  - Petrović, Rada
AU  - Janaćković, Đorđe
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6172
PB  - Serbian Academy of Sciences and Arts
C3  - Second International conference ELMINA2022 Electron Microscopy of Nanostructures
T1  - Improving the contact surface between TiO2 nanotubes and MAPbBr3 to make perovskite solar cells
EP  - 190
SP  - 189
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6172
ER  - 

@conference{
author = "Stefanović, Milica and Lukić, Ivana and Vujančević, Jelena and Petrović, Rada and Janaćković, Đorđe",
year = "2022",
publisher = "Serbian Academy of Sciences and Arts",
journal = "Second International conference ELMINA2022 Electron Microscopy of Nanostructures",
title = "Improving the contact surface between TiO2 nanotubes and MAPbBr3 to make perovskite solar cells",
pages = "190-189",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6172"
}

Stefanović, M., Lukić, I., Vujančević, J., Petrović, R.,& Janaćković, Đ.. (2022). Improving the contact surface between TiO2 nanotubes and MAPbBr3 to make perovskite solar cells. in Second International conference ELMINA2022 Electron Microscopy of Nanostructures
Serbian Academy of Sciences and Arts., 189-190.
https://hdl.handle.net/21.15107/rcub_technorep_6172

Stefanović M, Lukić I, Vujančević J, Petrović R, Janaćković Đ. Improving the contact surface between TiO2 nanotubes and MAPbBr3 to make perovskite solar cells. in Second International conference ELMINA2022 Electron Microscopy of Nanostructures. 2022;:189-190.
https://hdl.handle.net/21.15107/rcub_technorep_6172 .

Stefanović, Milica, Lukić, Ivana, Vujančević, Jelena, Petrović, Rada, Janaćković, Đorđe, "Improving the contact surface between TiO2 nanotubes and MAPbBr3 to make perovskite solar cells" in Second International conference ELMINA2022 Electron Microscopy of Nanostructures (2022):189-190,
https://hdl.handle.net/21.15107/rcub_technorep_6172 .

TY  - CONF
AU  - Lukić, Ivana
AU  - Milovanović, Stoja
AU  - Stamenić, Marko
AU  - Tadić, Vanja
AU  - Skala, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6985
AB  - Cannabis sativa is one of the oldest plant cultures, which has been used for centuries as a source of textile fibres, for paper production, as well as a source of bioactive compounds used in medicine. Although being restrained for a long time, its high potential, coupled with the progress in legislation and/or legalization in some regions of the world, led to the significant increase of scientific research towards isolation of cannabinoids, primarily a non-psychotropic cannabidiol (CBD), over the past couple decades. 
Beside cannabinoids, found only in cannabis plants, Cannabis sativa is characterized by very complex chemical composition with almost 500 constituents, including terpenes, flavonoids, phenolic compounds and fatty acids [2]. The co-presence of these numerous molecules in preparations obtained from Cannabis sativa may lead to a greater biological activity due to  the “entourage” or synergistic effect [3]. CBD has attracted considerable interest due to its numerous therapeutic properties and pharmacological activities [1]. However, cannabinoids are characterized by a low solubility in aqueous media and, as a consequence, a relatively poor bioavailability. Therefore, improvement of their stability, shelf-life and bioavailability, by incorporation into biocompatible carriers is still a challenge. 
As an answer to this issue, integrated process of supercritical fluid extraction (SFE) from Cannabis sativa and supercritical solvent impregnation (SSI) of obtained extract into starch aerogel was tested. Starch gels were prepared starting from hydrogel formed from an aqueous solution of cornstarch (1:10 w/v). During replacement of water with acetone, by successive increase of its concentration until 100%, acetogel was formed and further subjected to drying using supercritical CO2 (scCO2) at 45 °C and 8-10 MPa to obtain aerogel. For comparison, a part of acetogel was dried in the oven at 60 °C to obtain xerogel. Textural properties and morphology of prepared materials were determined by N2 adsorption–desorption isotherms and scanning electron microscopy (SEM) analysis. Aerogel with highest specific surface area of 208.6 m2/g, obtained at 45 °C and 10 MPa, was further used for extract impregnation. Integrated SFE-SSI process was performed at 40 °C and 10 MPa, conditions determined as optimal in the process of SFE of Cannabis sativa taking into account the yield of the obtained extract based on the composition, primarily regarding the CBD content. The amounts of impregnated extracts were 2.6 and 15.3% for xerogel and aerogel, respectively. The presence of the extract on the surface of impregnated samples was confirmed by FTIR analysis. Release study showed controlled release of extracts in PBS solution.
C3  - 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022
T1  - Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation
SP  - P-28
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6985
ER  - 

@conference{
author = "Lukić, Ivana and Milovanović, Stoja and Stamenić, Marko and Tadić, Vanja and Skala, Dejan",
year = "2022",
abstract = "Cannabis sativa is one of the oldest plant cultures, which has been used for centuries as a source of textile fibres, for paper production, as well as a source of bioactive compounds used in medicine. Although being restrained for a long time, its high potential, coupled with the progress in legislation and/or legalization in some regions of the world, led to the significant increase of scientific research towards isolation of cannabinoids, primarily a non-psychotropic cannabidiol (CBD), over the past couple decades. 
Beside cannabinoids, found only in cannabis plants, Cannabis sativa is characterized by very complex chemical composition with almost 500 constituents, including terpenes, flavonoids, phenolic compounds and fatty acids [2]. The co-presence of these numerous molecules in preparations obtained from Cannabis sativa may lead to a greater biological activity due to  the “entourage” or synergistic effect [3]. CBD has attracted considerable interest due to its numerous therapeutic properties and pharmacological activities [1]. However, cannabinoids are characterized by a low solubility in aqueous media and, as a consequence, a relatively poor bioavailability. Therefore, improvement of their stability, shelf-life and bioavailability, by incorporation into biocompatible carriers is still a challenge. 
As an answer to this issue, integrated process of supercritical fluid extraction (SFE) from Cannabis sativa and supercritical solvent impregnation (SSI) of obtained extract into starch aerogel was tested. Starch gels were prepared starting from hydrogel formed from an aqueous solution of cornstarch (1:10 w/v). During replacement of water with acetone, by successive increase of its concentration until 100%, acetogel was formed and further subjected to drying using supercritical CO2 (scCO2) at 45 °C and 8-10 MPa to obtain aerogel. For comparison, a part of acetogel was dried in the oven at 60 °C to obtain xerogel. Textural properties and morphology of prepared materials were determined by N2 adsorption–desorption isotherms and scanning electron microscopy (SEM) analysis. Aerogel with highest specific surface area of 208.6 m2/g, obtained at 45 °C and 10 MPa, was further used for extract impregnation. Integrated SFE-SSI process was performed at 40 °C and 10 MPa, conditions determined as optimal in the process of SFE of Cannabis sativa taking into account the yield of the obtained extract based on the composition, primarily regarding the CBD content. The amounts of impregnated extracts were 2.6 and 15.3% for xerogel and aerogel, respectively. The presence of the extract on the surface of impregnated samples was confirmed by FTIR analysis. Release study showed controlled release of extracts in PBS solution.",
journal = "2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022",
title = "Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation",
pages = "P-28",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6985"
}

Lukić, I., Milovanović, S., Stamenić, M., Tadić, V.,& Skala, D.. (2022). Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation. in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022, P-28.
https://hdl.handle.net/21.15107/rcub_technorep_6985

Lukić I, Milovanović S, Stamenić M, Tadić V, Skala D. Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation. in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022. 2022;:P-28.
https://hdl.handle.net/21.15107/rcub_technorep_6985 .

Lukić, Ivana, Milovanović, Stoja, Stamenić, Marko, Tadić, Vanja, Skala, Dejan, "Starch aerogels loaded with Cannabis sativa extract using integrated process of supercritical CO2 extraction and impregnation" in 2nd International Conference on Aerogels for Biomedical and Environmental Applications (AERoGELS2022), 29 June–1 July 2022, Athens, Greece, 2022 (2022):P-28,
https://hdl.handle.net/21.15107/rcub_technorep_6985 .

TY  - CONF
AU  - Lukić, Ivana
AU  - Pajnik, Jelena
AU  - Milovanović, Stoja
AU  - Tadić, Vanja
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6905
AB  - Bilberries (Vaccinium myrtillus) are believed to have one of the highest antioxidant levels of all
common fruits and vegetables. As a source of antioxidants, they have been used in traditional
medicine to reduce inflammation and protect against diseases associated with oxidative stress, such
as cardiovascular disease, diabetes, and age-related cognitive decline, as well as a remedy for eye
conditions. The biological activity of bilberries is associated with the high content of anthocyanins,
a large group of water-soluble flavonoids. Besides the complex anthocyanin profile, bilberries also
contain other phenolic compounds including flavonols, tannins, and phenolic acids. Supercritical fluid
extraction (SFE) isa a green and environmentally friendly process based on the use of supercritical
fluids, most commonly CO2, as an alternative to organic solvents, to obtain solvent-free and highly
valuable plant extracts. Utilization of supercritical CO2 (scCO2) extraction also enables the preservation
of thermally labile compounds working at low temperatures, and good selectivity accomplished by
the appropriate choice of operating pressure and temperature. In this study, scCO2 extraction was
investigated as a method for isolating the extract from bilberry dried fruit. Extractions were performed
at temperature of 70 °C and pressures of 15 and 30 MPa, with and without the ethanol as co-solvent,
with the aim to optimize the yield of the obtained extract based on the composition. The obtained
extraction yields ranged from 1.2 to 7% and the highest yield was obtained, as expected, at a higher
pressure of 30 MPa. The effect of ethanol as co-solvent in the scCO2 extraction was studied with the
goal to modify scCO2 selectivity and enhance the solubility of polar substances. The results showed a
positive effect on the extraction yield, while total phenolic content (TPC) analysis revealed that extracts
obtained using co-solvent have higher TPC values. Chemical analysis of the obtained extracts was
performed in order to qualitatively evaluate their composition.
PB  - Zagreb : University, Faculty of Food Technology and Biotechnology
C3  - Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022
T1  - SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT
SP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6905
ER  - 

@conference{
author = "Lukić, Ivana and Pajnik, Jelena and Milovanović, Stoja and Tadić, Vanja",
year = "2022",
abstract = "Bilberries (Vaccinium myrtillus) are believed to have one of the highest antioxidant levels of all
common fruits and vegetables. As a source of antioxidants, they have been used in traditional
medicine to reduce inflammation and protect against diseases associated with oxidative stress, such
as cardiovascular disease, diabetes, and age-related cognitive decline, as well as a remedy for eye
conditions. The biological activity of bilberries is associated with the high content of anthocyanins,
a large group of water-soluble flavonoids. Besides the complex anthocyanin profile, bilberries also
contain other phenolic compounds including flavonols, tannins, and phenolic acids. Supercritical fluid
extraction (SFE) isa a green and environmentally friendly process based on the use of supercritical
fluids, most commonly CO2, as an alternative to organic solvents, to obtain solvent-free and highly
valuable plant extracts. Utilization of supercritical CO2 (scCO2) extraction also enables the preservation
of thermally labile compounds working at low temperatures, and good selectivity accomplished by
the appropriate choice of operating pressure and temperature. In this study, scCO2 extraction was
investigated as a method for isolating the extract from bilberry dried fruit. Extractions were performed
at temperature of 70 °C and pressures of 15 and 30 MPa, with and without the ethanol as co-solvent,
with the aim to optimize the yield of the obtained extract based on the composition. The obtained
extraction yields ranged from 1.2 to 7% and the highest yield was obtained, as expected, at a higher
pressure of 30 MPa. The effect of ethanol as co-solvent in the scCO2 extraction was studied with the
goal to modify scCO2 selectivity and enhance the solubility of polar substances. The results showed a
positive effect on the extraction yield, while total phenolic content (TPC) analysis revealed that extracts
obtained using co-solvent have higher TPC values. Chemical analysis of the obtained extracts was
performed in order to qualitatively evaluate their composition.",
publisher = "Zagreb : University, Faculty of Food Technology and Biotechnology",
journal = "Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022",
title = "SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT",
pages = "100",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6905"
}

Lukić, I., Pajnik, J., Milovanović, S.,& Tadić, V.. (2022). SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT. in Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022
Zagreb : University, Faculty of Food Technology and Biotechnology., 100.
https://hdl.handle.net/21.15107/rcub_technorep_6905

Lukić I, Pajnik J, Milovanović S, Tadić V. SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT. in Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022. 2022;:100.
https://hdl.handle.net/21.15107/rcub_technorep_6905 .

Lukić, Ivana, Pajnik, Jelena, Milovanović, Stoja, Tadić, Vanja, "SUPERCRITICAL CO2 EXTRACTION OF BILBERRY (Vaccinium myrtillus) FRUIT" in Book of Abstracts / 4th Natural resources, green technology and sustainable development/ 4- GREEN2022, Zagreb, 14th - 16th September 2022 (2022):100,
https://hdl.handle.net/21.15107/rcub_technorep_6905 .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Pajnik, Jelena
AU  - Lukić, Ivana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4982
AB  - Poly(lactic acid) (PLA) stands out as the most promising biodegradable alternative to conventional petrochemical-based polymers for manufacturing of high-performance materials applied in medicine, pharmacy, food, textile, and electronic industry. This review was aimed to present the conventional and up-to-date technologies for PLA processing including melt blending and molding, hot melt extrusion, 3D printing, foaming, impregnation, thermally induced phase separation, nano- and microparticles preparation, wet and dry spinning processes. In addition, the effect of the processing parameters and polymer characteristics on the properties of the final material was elaborated. Diverse possibilities to tailor properties of PLA-based materials by variation in polymer characteristics and concentration, solvent selection, drying method, processing pressure and temperature, incorporation of bioactive components, and so on were highlighted. The examples of the relations between processing methods, parameters, and end-product properties are given for a better understanding of all aspects that need to be perceived for fabrication of PLA-based materials with required performances.
T2  - Journal of Applied Polymer Science
T1  - Tailoring of advanced poly(lactic acid)-based materials: A review
IS  - 12
VL  - 139
DO  - 10.1002/app.51839
ER  - 

@article{
author = "Milovanović, Stoja and Pajnik, Jelena and Lukić, Ivana",
year = "2022",
abstract = "Poly(lactic acid) (PLA) stands out as the most promising biodegradable alternative to conventional petrochemical-based polymers for manufacturing of high-performance materials applied in medicine, pharmacy, food, textile, and electronic industry. This review was aimed to present the conventional and up-to-date technologies for PLA processing including melt blending and molding, hot melt extrusion, 3D printing, foaming, impregnation, thermally induced phase separation, nano- and microparticles preparation, wet and dry spinning processes. In addition, the effect of the processing parameters and polymer characteristics on the properties of the final material was elaborated. Diverse possibilities to tailor properties of PLA-based materials by variation in polymer characteristics and concentration, solvent selection, drying method, processing pressure and temperature, incorporation of bioactive components, and so on were highlighted. The examples of the relations between processing methods, parameters, and end-product properties are given for a better understanding of all aspects that need to be perceived for fabrication of PLA-based materials with required performances.",
journal = "Journal of Applied Polymer Science",
title = "Tailoring of advanced poly(lactic acid)-based materials: A review",
number = "12",
volume = "139",
doi = "10.1002/app.51839"
}

Milovanović, S., Pajnik, J.,& Lukić, I.. (2022). Tailoring of advanced poly(lactic acid)-based materials: A review. in Journal of Applied Polymer Science, 139(12).
https://doi.org/10.1002/app.51839

Milovanović S, Pajnik J, Lukić I. Tailoring of advanced poly(lactic acid)-based materials: A review. in Journal of Applied Polymer Science. 2022;139(12).
doi:10.1002/app.51839 .

Milovanović, Stoja, Pajnik, Jelena, Lukić, Ivana, "Tailoring of advanced poly(lactic acid)-based materials: A review" in Journal of Applied Polymer Science, 139, no. 12 (2022),
https://doi.org/10.1002/app.51839 . .

TY  - JOUR
AU  - Lukić, Ivana
AU  - Milovanović, Stoja
AU  - Pantić, Milica
AU  - Srbljak, Ivana
AU  - Djurić, Ana
AU  - Tadić, Vanja
AU  - Tyśkiewicz, Katarzyna
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5093
AB  - Extracts from Silybum marianum seeds have high industrial potential due to their application as phytopharmaceuticals and food. In order to optimize its production, conventional extraction (in Soxhlet apparatus using solvents ethanol and n-hexane) and supercritical fluid extraction (using CO2 at the pressure of 30 MPa and temperature of 40 °C with and without co-solvent ethanol, from oil-rich and defatted seeds) were compared. In addition, the effect of plant cultivation (years 2011 and 2019) and the effect of seeds and extract storage on the yield and quality of extracts were assessed. It was shown that unsaturated fatty acids (linoleic and oleic acids being most dominant) constituted 64–87% of obtained extracts. Content of α-tocopherol varied from 0.01 to 13 g/100 g, while total phenolic content was in the range from 13.2 to 104.2 gGAE/100 gextract. In vitro cytotoxic activity analysis confirmed that extracts obtained from defatted seeds showed activity against cancer cells. This study pointed out the important role of the cultivation year, selection of extraction technique and solvent, as well as storage on the extraction yield, chemical profile, and cytotoxic activity of extracts.
PB  - Academic Press
T2  - LWT
T1  - Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity
SP  - 113319
VL  - 160
DO  - 10.1016/j.lwt.2022.113319
ER  - 

@article{
author = "Lukić, Ivana and Milovanović, Stoja and Pantić, Milica and Srbljak, Ivana and Djurić, Ana and Tadić, Vanja and Tyśkiewicz, Katarzyna",
year = "2022",
abstract = "Extracts from Silybum marianum seeds have high industrial potential due to their application as phytopharmaceuticals and food. In order to optimize its production, conventional extraction (in Soxhlet apparatus using solvents ethanol and n-hexane) and supercritical fluid extraction (using CO2 at the pressure of 30 MPa and temperature of 40 °C with and without co-solvent ethanol, from oil-rich and defatted seeds) were compared. In addition, the effect of plant cultivation (years 2011 and 2019) and the effect of seeds and extract storage on the yield and quality of extracts were assessed. It was shown that unsaturated fatty acids (linoleic and oleic acids being most dominant) constituted 64–87% of obtained extracts. Content of α-tocopherol varied from 0.01 to 13 g/100 g, while total phenolic content was in the range from 13.2 to 104.2 gGAE/100 gextract. In vitro cytotoxic activity analysis confirmed that extracts obtained from defatted seeds showed activity against cancer cells. This study pointed out the important role of the cultivation year, selection of extraction technique and solvent, as well as storage on the extraction yield, chemical profile, and cytotoxic activity of extracts.",
publisher = "Academic Press",
journal = "LWT",
title = "Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity",
pages = "113319",
volume = "160",
doi = "10.1016/j.lwt.2022.113319"
}

Lukić, I., Milovanović, S., Pantić, M., Srbljak, I., Djurić, A., Tadić, V.,& Tyśkiewicz, K.. (2022). Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity. in LWT
Academic Press., 160, 113319.
https://doi.org/10.1016/j.lwt.2022.113319

Lukić I, Milovanović S, Pantić M, Srbljak I, Djurić A, Tadić V, Tyśkiewicz K. Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity. in LWT. 2022;160:113319.
doi:10.1016/j.lwt.2022.113319 .

Lukić, Ivana, Milovanović, Stoja, Pantić, Milica, Srbljak, Ivana, Djurić, Ana, Tadić, Vanja, Tyśkiewicz, Katarzyna, "Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity" in LWT, 160 (2022):113319,
https://doi.org/10.1016/j.lwt.2022.113319 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Lukić, Ivana
AU  - Stamenić, Marko
AU  - Kamiński, Piotr
AU  - Florkowski, Grzegorz
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5175
AB  - The present study was focused on improving the supercritical fluid extraction process dedicated to the recovery of highly valuable extract from Silybum marianum seeds. At first, the process was tested using supercritical CO2 at temperatures of 40 and 80 °C and pressures ranging from 100 to 300 bar. Obtained values were described using two literature models with acceptable agreement of experimental and calculated data. Further, the effect of the equipment design and process scale-up was investigated in four high-pressure units having volumes in the range from 280 mL to 40 L resulting in extraction yields up to 26 %. Based on the values of the parameters determined at the laboratory scale, the extraction process was successfully transferred to a semi-industrial scale. Finally, estimated costs for supercritical extract production in Poland for the year 2021 using a 1000 L high-pressure unit amounted to the price of 35.9 EUR per kg of extract.
PB  - Elsevier B.V.
T2  - Journal of Supercritical Fluids
T1  - The effect of equipment design and process scale-up on supercritical CO2 extraction: Case study for Silybum marianum seeds
SP  - 105676
VL  - 188
DO  - 10.1016/j.supflu.2022.105676
ER  - 

@article{
author = "Milovanović, Stoja and Lukić, Ivana and Stamenić, Marko and Kamiński, Piotr and Florkowski, Grzegorz and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2022",
abstract = "The present study was focused on improving the supercritical fluid extraction process dedicated to the recovery of highly valuable extract from Silybum marianum seeds. At first, the process was tested using supercritical CO2 at temperatures of 40 and 80 °C and pressures ranging from 100 to 300 bar. Obtained values were described using two literature models with acceptable agreement of experimental and calculated data. Further, the effect of the equipment design and process scale-up was investigated in four high-pressure units having volumes in the range from 280 mL to 40 L resulting in extraction yields up to 26 %. Based on the values of the parameters determined at the laboratory scale, the extraction process was successfully transferred to a semi-industrial scale. Finally, estimated costs for supercritical extract production in Poland for the year 2021 using a 1000 L high-pressure unit amounted to the price of 35.9 EUR per kg of extract.",
publisher = "Elsevier B.V.",
journal = "Journal of Supercritical Fluids",
title = "The effect of equipment design and process scale-up on supercritical CO2 extraction: Case study for Silybum marianum seeds",
pages = "105676",
volume = "188",
doi = "10.1016/j.supflu.2022.105676"
}

Milovanović, S., Lukić, I., Stamenić, M., Kamiński, P., Florkowski, G., Tyśkiewicz, K.,& Konkol, M.. (2022). The effect of equipment design and process scale-up on supercritical CO2 extraction: Case study for Silybum marianum seeds. in Journal of Supercritical Fluids
Elsevier B.V.., 188, 105676.
https://doi.org/10.1016/j.supflu.2022.105676

Milovanović S, Lukić I, Stamenić M, Kamiński P, Florkowski G, Tyśkiewicz K, Konkol M. The effect of equipment design and process scale-up on supercritical CO2 extraction: Case study for Silybum marianum seeds. in Journal of Supercritical Fluids. 2022;188:105676.
doi:10.1016/j.supflu.2022.105676 .

Milovanović, Stoja, Lukić, Ivana, Stamenić, Marko, Kamiński, Piotr, Florkowski, Grzegorz, Tyśkiewicz, Katarzyna, Konkol, Marcin, "The effect of equipment design and process scale-up on supercritical CO2 extraction: Case study for Silybum marianum seeds" in Journal of Supercritical Fluids, 188 (2022):105676,
https://doi.org/10.1016/j.supflu.2022.105676 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Lukić, Ivana
AU  - Kaminski, Piotr
AU  - Debczak, Agnieszka
AU  - Klimkowska, Kamila
AU  - Tyskiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5190
AB  - The aim of this study was to provide insights into parameters that affect the oil extraction from an industrially valuable raw material (milk thistle) using green solvent supercritical CO2. The effect of plant origin, cultivation year, and seeds storage on extraction yield has been explored. Furthermore, the effects of high-pressure unit design and initial plant material amount have been examined. Pressure (200-450 bar) and temperature (40-80 °C) have been tested to improve the recovery of essential fatty acids and phenolic compounds. Moreover, solid residues after the material pretreatment and supercritical CO2 extraction served as the substrates for waste valorization. The characterization of obtained high-value extracts has been carried out by chromatographic (using gas chromatographs combined with mass spectrometer and flame ionization detection) and spectrophotometric assays (for total phenolic and flavonoid compounds as well as a radical scavenging ability). It was shown that the tested supercritical CO2 extraction process parameters determined extraction kinetics and allowed the extract separation from raw milk thistle seeds and waste material (3-32 mass %) rich in linoleic acid (up to 515 mg/g) and total phenolic compounds (up to 30 mg GAE/g).
PB  - Elsevier Ltd
T2  - Journal of CO2 Utilization
T1  - Green manufacturing of high-value extracts from milk thistle seeds: Parameters that affect the supercritical CO2extraction process
SP  - 102134
VL  - 63
DO  - 10.1016/j.jcou.2022.102134
ER  - 

@article{
author = "Milovanović, Stoja and Lukić, Ivana and Kaminski, Piotr and Debczak, Agnieszka and Klimkowska, Kamila and Tyskiewicz, Katarzyna and Konkol, Marcin",
year = "2022",
abstract = "The aim of this study was to provide insights into parameters that affect the oil extraction from an industrially valuable raw material (milk thistle) using green solvent supercritical CO2. The effect of plant origin, cultivation year, and seeds storage on extraction yield has been explored. Furthermore, the effects of high-pressure unit design and initial plant material amount have been examined. Pressure (200-450 bar) and temperature (40-80 °C) have been tested to improve the recovery of essential fatty acids and phenolic compounds. Moreover, solid residues after the material pretreatment and supercritical CO2 extraction served as the substrates for waste valorization. The characterization of obtained high-value extracts has been carried out by chromatographic (using gas chromatographs combined with mass spectrometer and flame ionization detection) and spectrophotometric assays (for total phenolic and flavonoid compounds as well as a radical scavenging ability). It was shown that the tested supercritical CO2 extraction process parameters determined extraction kinetics and allowed the extract separation from raw milk thistle seeds and waste material (3-32 mass %) rich in linoleic acid (up to 515 mg/g) and total phenolic compounds (up to 30 mg GAE/g).",
publisher = "Elsevier Ltd",
journal = "Journal of CO2 Utilization",
title = "Green manufacturing of high-value extracts from milk thistle seeds: Parameters that affect the supercritical CO2extraction process",
pages = "102134",
volume = "63",
doi = "10.1016/j.jcou.2022.102134"
}

Milovanović, S., Lukić, I., Kaminski, P., Debczak, A., Klimkowska, K., Tyskiewicz, K.,& Konkol, M.. (2022). Green manufacturing of high-value extracts from milk thistle seeds: Parameters that affect the supercritical CO2extraction process. in Journal of CO2 Utilization
Elsevier Ltd., 63, 102134.
https://doi.org/10.1016/j.jcou.2022.102134

Milovanović S, Lukić I, Kaminski P, Debczak A, Klimkowska K, Tyskiewicz K, Konkol M. Green manufacturing of high-value extracts from milk thistle seeds: Parameters that affect the supercritical CO2extraction process. in Journal of CO2 Utilization. 2022;63:102134.
doi:10.1016/j.jcou.2022.102134 .

Milovanović, Stoja, Lukić, Ivana, Kaminski, Piotr, Debczak, Agnieszka, Klimkowska, Kamila, Tyskiewicz, Katarzyna, Konkol, Marcin, "Green manufacturing of high-value extracts from milk thistle seeds: Parameters that affect the supercritical CO2extraction process" in Journal of CO2 Utilization, 63 (2022):102134,
https://doi.org/10.1016/j.jcou.2022.102134 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Lukić, Ivana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5962
AB  - Supercritical carbon dioxide (scCO2) application in the pharmaceutical industry is still undeveloped regardless of significant research interests in this processing medium shown in the last decades. ScCO2 technologies can improve drug solubility, bioavailability, and therapeutic effect. These technologies can lead to the development of new formulations that will contribute to a decrease in drug dose, medication frequency, and increase patients’ well-being. Considering the significant decrease in the price of high-pressure equipment and society's growing need for cleaner production and safer products, it is expected that symbiosis between supercritical fluid and pharmaceutical technologies will happen soon. Therefore, this review was focused on the latest contributions of scCO2 technologies to the pharmaceutical field. The main aim was to bring these technologies closer to pharmaceutical specialists. For this purpose, the most commonly used technologies were explained and discussed: the preparation of solid dispersions, polymer impregnation with drugs, and drug micro/nanoparticle production using scCO2.
PB  - Pharmaceutical Association of Serbia
T2  - Arhiv za Farmaciju
T1  - An overview on the application of supercritical carbon dioxide for the processing of pharmaceuticals
T1  - Pregled mogućnosti primene natkritičnog ugljenik(IV)-oksida u proizvodnji lekova
EP  - 590
IS  - 6
SP  - 566
VL  - 72
DO  - 10.5937/ARHFARM72-39999
ER  - 

@article{
author = "Milovanović, Stoja and Lukić, Ivana",
year = "2022",
abstract = "Supercritical carbon dioxide (scCO2) application in the pharmaceutical industry is still undeveloped regardless of significant research interests in this processing medium shown in the last decades. ScCO2 technologies can improve drug solubility, bioavailability, and therapeutic effect. These technologies can lead to the development of new formulations that will contribute to a decrease in drug dose, medication frequency, and increase patients’ well-being. Considering the significant decrease in the price of high-pressure equipment and society's growing need for cleaner production and safer products, it is expected that symbiosis between supercritical fluid and pharmaceutical technologies will happen soon. Therefore, this review was focused on the latest contributions of scCO2 technologies to the pharmaceutical field. The main aim was to bring these technologies closer to pharmaceutical specialists. For this purpose, the most commonly used technologies were explained and discussed: the preparation of solid dispersions, polymer impregnation with drugs, and drug micro/nanoparticle production using scCO2.",
publisher = "Pharmaceutical Association of Serbia",
journal = "Arhiv za Farmaciju",
title = "An overview on the application of supercritical carbon dioxide for the processing of pharmaceuticals, Pregled mogućnosti primene natkritičnog ugljenik(IV)-oksida u proizvodnji lekova",
pages = "590-566",
number = "6",
volume = "72",
doi = "10.5937/ARHFARM72-39999"
}

Milovanović, S.,& Lukić, I.. (2022). An overview on the application of supercritical carbon dioxide for the processing of pharmaceuticals. in Arhiv za Farmaciju
Pharmaceutical Association of Serbia., 72(6), 566-590.
https://doi.org/10.5937/ARHFARM72-39999

Milovanović S, Lukić I. An overview on the application of supercritical carbon dioxide for the processing of pharmaceuticals. in Arhiv za Farmaciju. 2022;72(6):566-590.
doi:10.5937/ARHFARM72-39999 .

Milovanović, Stoja, Lukić, Ivana, "An overview on the application of supercritical carbon dioxide for the processing of pharmaceuticals" in Arhiv za Farmaciju, 72, no. 6 (2022):566-590,
https://doi.org/10.5937/ARHFARM72-39999 . .

TY  - JOUR
AU  - Lukić, Ivana
AU  - Pajnik, Jelena
AU  - Nišavić, Jakov
AU  - Tadić, Vanja
AU  - Vági, Erika
AU  - Szekely, Edit
AU  - Žižović, Irena
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5113
AB  - The integrated supercritical fluid extraction–impregnation process (SFE-SSI) was performed to fabricate material with antiviral properties against the herpes simplex virus (HSV). Cotton gauze and starch/chitosan polymer films (SCF) were impregnated with components extracted from Melissa officinalis at 10 MPa and 40 °C using a green medium, supercritical carbon dioxide (scCO2). The influences of the processing mode regarding the flow of the supercritical fluid through the system, and the mass ratio of the plant material and the solid carrier, on the impregnation yield of M. officinalis extract were studied. The results revealed that the introduction of a fresh amount of CO2 into the system enabled the highest impregnation yield of 2.24% for cotton gauze and 8.71% for SCF. The presence of M. officinalis extract on the surface of both impregnated cotton gaze and SCF was confirmed by FTIR and GC analyses after the re-extraction of the impregnated samples. The M. officinalis impregnated materials showed a strong inhibitory effect against Bovine herpesvirus type 1 (BHV-1).
PB  - MDPI
T2  - Processes
T1  - Application of the Integrated Supercritical Fluid Extraction–Impregnation Process (SFE-SSI) for Development of Materials with Antiviral Properties
IS  - 4
SP  - 680
VL  - 10
DO  - 10.3390/pr10040680
ER  - 

@article{
author = "Lukić, Ivana and Pajnik, Jelena and Nišavić, Jakov and Tadić, Vanja and Vági, Erika and Szekely, Edit and Žižović, Irena",
year = "2022",
abstract = "The integrated supercritical fluid extraction–impregnation process (SFE-SSI) was performed to fabricate material with antiviral properties against the herpes simplex virus (HSV). Cotton gauze and starch/chitosan polymer films (SCF) were impregnated with components extracted from Melissa officinalis at 10 MPa and 40 °C using a green medium, supercritical carbon dioxide (scCO2). The influences of the processing mode regarding the flow of the supercritical fluid through the system, and the mass ratio of the plant material and the solid carrier, on the impregnation yield of M. officinalis extract were studied. The results revealed that the introduction of a fresh amount of CO2 into the system enabled the highest impregnation yield of 2.24% for cotton gauze and 8.71% for SCF. The presence of M. officinalis extract on the surface of both impregnated cotton gaze and SCF was confirmed by FTIR and GC analyses after the re-extraction of the impregnated samples. The M. officinalis impregnated materials showed a strong inhibitory effect against Bovine herpesvirus type 1 (BHV-1).",
publisher = "MDPI",
journal = "Processes",
title = "Application of the Integrated Supercritical Fluid Extraction–Impregnation Process (SFE-SSI) for Development of Materials with Antiviral Properties",
number = "4",
pages = "680",
volume = "10",
doi = "10.3390/pr10040680"
}

Lukić, I., Pajnik, J., Nišavić, J., Tadić, V., Vági, E., Szekely, E.,& Žižović, I.. (2022). Application of the Integrated Supercritical Fluid Extraction–Impregnation Process (SFE-SSI) for Development of Materials with Antiviral Properties. in Processes
MDPI., 10(4), 680.
https://doi.org/10.3390/pr10040680

Lukić I, Pajnik J, Nišavić J, Tadić V, Vági E, Szekely E, Žižović I. Application of the Integrated Supercritical Fluid Extraction–Impregnation Process (SFE-SSI) for Development of Materials with Antiviral Properties. in Processes. 2022;10(4):680.
doi:10.3390/pr10040680 .

Lukić, Ivana, Pajnik, Jelena, Nišavić, Jakov, Tadić, Vanja, Vági, Erika, Szekely, Edit, Žižović, Irena, "Application of the Integrated Supercritical Fluid Extraction–Impregnation Process (SFE-SSI) for Development of Materials with Antiviral Properties" in Processes, 10, no. 4 (2022):680,
https://doi.org/10.3390/pr10040680 . .

TY  - CONF
AU  - Stefanović, Milica
AU  - Petrović, Rada
AU  - Lukić, Ivana
AU  - Janaćković, Đorđe
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6163
AB  - Organo-inorganic perovskites have attracted much attention of researchers due to their low-cost fabrication and high optical absorption. When perovskite materials were applied for the first time in solar cells (2006), power conversion efficiency (PCE) was 2.2 %. Up to now, PCE is over 25.5 %. Despite the conveniences that perovskites have, there are problems regarding the stability of the material and the recombination of charge carriers. To decrease charge carriers recombination, perovskite methylammonium lead bromide (MAPbBr3) was coupled with TiO2 nanotube arrays which were synthesized by anodisation of Ti foil and annealed at 450 °C for 1 h. The inner diameter of the nanotubes was ~ 103 ± 17 nm while the length was ~ 350 nm. The solution of MAPbBr3 in dimethylformamide (DMF) was deposited on TiO2 nanotubes by supercritical carbon dioxide at different pressures and temperatures. The supercritical CO2 has the role of a cosolvent that would help to fill the nanotubes with perovskite material. It has been observed that supercritical CO2 improves the filling of nanotubes by the perovskite. Morphological and chemical characterization of samples was carried out by FESEM and XRD analysis. The diffuse reflectance spectroscopy measurement of samples proved that the absorption edge of prepared TiO2 nanotubes/MAPbBr3 was extended to the visible range. By measuring the I-V characteristics of these samples in the dark and under visible light, a hysteresis curve was obtained with values of current in the range of 40 to 600 μA.
PB  - Faculty of Technology, University of Novi Sad
C3  - 14th ECERS Conference for Young Scientists in Ceramics 2021
T1  - Deposition of methylammonium lead bromide perovskite on titania nanotube arrays assisted by supercritical carbon dioxide
SP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6163
ER  - 

@conference{
author = "Stefanović, Milica and Petrović, Rada and Lukić, Ivana and Janaćković, Đorđe",
year = "2021",
abstract = "Organo-inorganic perovskites have attracted much attention of researchers due to their low-cost fabrication and high optical absorption. When perovskite materials were applied for the first time in solar cells (2006), power conversion efficiency (PCE) was 2.2 %. Up to now, PCE is over 25.5 %. Despite the conveniences that perovskites have, there are problems regarding the stability of the material and the recombination of charge carriers. To decrease charge carriers recombination, perovskite methylammonium lead bromide (MAPbBr3) was coupled with TiO2 nanotube arrays which were synthesized by anodisation of Ti foil and annealed at 450 °C for 1 h. The inner diameter of the nanotubes was ~ 103 ± 17 nm while the length was ~ 350 nm. The solution of MAPbBr3 in dimethylformamide (DMF) was deposited on TiO2 nanotubes by supercritical carbon dioxide at different pressures and temperatures. The supercritical CO2 has the role of a cosolvent that would help to fill the nanotubes with perovskite material. It has been observed that supercritical CO2 improves the filling of nanotubes by the perovskite. Morphological and chemical characterization of samples was carried out by FESEM and XRD analysis. The diffuse reflectance spectroscopy measurement of samples proved that the absorption edge of prepared TiO2 nanotubes/MAPbBr3 was extended to the visible range. By measuring the I-V characteristics of these samples in the dark and under visible light, a hysteresis curve was obtained with values of current in the range of 40 to 600 μA.",
publisher = "Faculty of Technology, University of Novi Sad",
journal = "14th ECERS Conference for Young Scientists in Ceramics 2021",
title = "Deposition of methylammonium lead bromide perovskite on titania nanotube arrays assisted by supercritical carbon dioxide",
pages = "75",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6163"
}

Stefanović, M., Petrović, R., Lukić, I.,& Janaćković, Đ.. (2021). Deposition of methylammonium lead bromide perovskite on titania nanotube arrays assisted by supercritical carbon dioxide. in 14th ECERS Conference for Young Scientists in Ceramics 2021
Faculty of Technology, University of Novi Sad., 75.
https://hdl.handle.net/21.15107/rcub_technorep_6163

Stefanović M, Petrović R, Lukić I, Janaćković Đ. Deposition of methylammonium lead bromide perovskite on titania nanotube arrays assisted by supercritical carbon dioxide. in 14th ECERS Conference for Young Scientists in Ceramics 2021. 2021;:75.
https://hdl.handle.net/21.15107/rcub_technorep_6163 .

Stefanović, Milica, Petrović, Rada, Lukić, Ivana, Janaćković, Đorđe, "Deposition of methylammonium lead bromide perovskite on titania nanotube arrays assisted by supercritical carbon dioxide" in 14th ECERS Conference for Young Scientists in Ceramics 2021 (2021):75,
https://hdl.handle.net/21.15107/rcub_technorep_6163 .

TY  - CONF
AU  - Stefanović, Milica
AU  - Petrović, Rada
AU  - Lukić, Ivana
AU  - Vujančević, Jelena
AU  - Janaćković, Đorđe
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6166
PB  - Institute of Technical Sciences of SASA
C3  - 19th Young Researchers’ Conference Materials Science And Engineering 2021
T1  - Application of supercritical carbon dioxide for making perovskite photodiode
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6166
ER  - 

@conference{
author = "Stefanović, Milica and Petrović, Rada and Lukić, Ivana and Vujančević, Jelena and Janaćković, Đorđe",
year = "2021",
publisher = "Institute of Technical Sciences of SASA",
journal = "19th Young Researchers’ Conference Materials Science And Engineering 2021",
title = "Application of supercritical carbon dioxide for making perovskite photodiode",
pages = "70",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6166"
}

Stefanović, M., Petrović, R., Lukić, I., Vujančević, J.,& Janaćković, Đ.. (2021). Application of supercritical carbon dioxide for making perovskite photodiode. in 19th Young Researchers’ Conference Materials Science And Engineering 2021
Institute of Technical Sciences of SASA., 70.
https://hdl.handle.net/21.15107/rcub_technorep_6166

Stefanović M, Petrović R, Lukić I, Vujančević J, Janaćković Đ. Application of supercritical carbon dioxide for making perovskite photodiode. in 19th Young Researchers’ Conference Materials Science And Engineering 2021. 2021;:70.
https://hdl.handle.net/21.15107/rcub_technorep_6166 .

Stefanović, Milica, Petrović, Rada, Lukić, Ivana, Vujančević, Jelena, Janaćković, Đorđe, "Application of supercritical carbon dioxide for making perovskite photodiode" in 19th Young Researchers’ Conference Materials Science And Engineering 2021 (2021):70,
https://hdl.handle.net/21.15107/rcub_technorep_6166 .