TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2024-03
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7319
AB  - A crucial aspect of tissue self-organization during morphogenesis, wound healing, and cancer invasion is directed migration of cell collectives. The majority of in vivo directed migration has been guided by chemotaxis, whereby cells follow a chemical gradient. In certain situations, migrating cell collectives can also self-generate the stiffness gradient in the surrounding tissue, which can have a feedback effect on the directionality of the migration. The phenomenon has been observed during collective durotaxis in vivo. Along the biointerface between neighbouring tissues, heterotypic cell-cell interactions are the main cause of this self-generated stiffness gradient. The physical processes in charge of tissue self-organization along the biointerface, which are related to the interplay between cell signalling and the formation of heterotypic cell-cell adhesion contacts, are less well-developed than the biological mechanisms of the cellular interactions. This complex phenomenon is discussed here in the model system, such as collective migration of neural crest cells between ectodermal placode and mesoderm subpopulations within Xenopus embryos by pointing to the role of the dynamics along the biointerface between adjacent cell subpopulations on the subpopulation stiffness.
PB  - Elsevier B.V.
T2  - Biosystems
T1  - Collective durotaxis along a self-generated mobile stiffness gradient in vivo
SP  - 105155
VL  - 237
DO  - 10.1016/j.biosystems.2024.105155
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2024-03",
abstract = "A crucial aspect of tissue self-organization during morphogenesis, wound healing, and cancer invasion is directed migration of cell collectives. The majority of in vivo directed migration has been guided by chemotaxis, whereby cells follow a chemical gradient. In certain situations, migrating cell collectives can also self-generate the stiffness gradient in the surrounding tissue, which can have a feedback effect on the directionality of the migration. The phenomenon has been observed during collective durotaxis in vivo. Along the biointerface between neighbouring tissues, heterotypic cell-cell interactions are the main cause of this self-generated stiffness gradient. The physical processes in charge of tissue self-organization along the biointerface, which are related to the interplay between cell signalling and the formation of heterotypic cell-cell adhesion contacts, are less well-developed than the biological mechanisms of the cellular interactions. This complex phenomenon is discussed here in the model system, such as collective migration of neural crest cells between ectodermal placode and mesoderm subpopulations within Xenopus embryos by pointing to the role of the dynamics along the biointerface between adjacent cell subpopulations on the subpopulation stiffness.",
publisher = "Elsevier B.V.",
journal = "Biosystems",
title = "Collective durotaxis along a self-generated mobile stiffness gradient in vivo",
pages = "105155",
volume = "237",
doi = "10.1016/j.biosystems.2024.105155"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2024-03). Collective durotaxis along a self-generated mobile stiffness gradient in vivo. in Biosystems
Elsevier B.V.., 237, 105155.
https://doi.org/10.1016/j.biosystems.2024.105155

Pajić-Lijaković I, Milivojević M. Collective durotaxis along a self-generated mobile stiffness gradient in vivo. in Biosystems. 2024;237:105155.
doi:10.1016/j.biosystems.2024.105155 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Collective durotaxis along a self-generated mobile stiffness gradient in vivo" in Biosystems, 237 (2024-03):105155,
https://doi.org/10.1016/j.biosystems.2024.105155 . .

TY  - CHAP
AU  - Milivojević, Milan
AU  - Pajić-Lijaković, Ivana
AU  - Dajić, Zora
AU  - Dhara, Amal Kumar
AU  - Nayak, Amit Kumar
AU  - Hasnain, Md Saquib
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7431
AB  - In many cases, conventional therapeutics are not able to cure diseases in a proper and safe manner. Conversely, plants and their derivatives have been successfully used for the treatment of numerous diseases and disorders for centuries and have proven themselves as efficient, inexpensive, environmentally friendly, faster, and less toxic. However, their use is sometimes connected to some issues, mainly due to inefficient systemic delivery and bioavailability, which prevent to translate their promising in vitro and in vivo effects into clinical use. During the past decade, nanotechnology has made big progress in developing different nanocarriers for drug delivery formulations. In the case of phytopharmaceuticals their encapsulation in nanoparticles can help in improving drug solubility, physical and chemical stability, pharmacological activity, reducing its toxicity and side effects, and providing targeted and sustained delivery which all leads to better bioavailability. The current review highlights the main problems connected to the delivery of phytopharmaceuticals, the main properties of different types of plant drugs, and gives a critical review of properties and limitations connected to the application of numerous lipid, polymer, and inorganic nanoparticles as phytodrug carriers. It also provides information about up-to-date investigated combinations of phytopharmaceuticals and nanocarriers.
PB  - Singapore : Springer
T2  - Role of Herbal Medicines
T1  - Nanotechnology in Delivery and Targeting of Phytochemicals for Lifestyle Diseases
EP  - 524
SP  - 497
DO  - 10.1007/978-981-99-7703-1_25
ER  - 

@inbook{
author = "Milivojević, Milan and Pajić-Lijaković, Ivana and Dajić, Zora and Dhara, Amal Kumar and Nayak, Amit Kumar and Hasnain, Md Saquib",
year = "2024",
abstract = "In many cases, conventional therapeutics are not able to cure diseases in a proper and safe manner. Conversely, plants and their derivatives have been successfully used for the treatment of numerous diseases and disorders for centuries and have proven themselves as efficient, inexpensive, environmentally friendly, faster, and less toxic. However, their use is sometimes connected to some issues, mainly due to inefficient systemic delivery and bioavailability, which prevent to translate their promising in vitro and in vivo effects into clinical use. During the past decade, nanotechnology has made big progress in developing different nanocarriers for drug delivery formulations. In the case of phytopharmaceuticals their encapsulation in nanoparticles can help in improving drug solubility, physical and chemical stability, pharmacological activity, reducing its toxicity and side effects, and providing targeted and sustained delivery which all leads to better bioavailability. The current review highlights the main problems connected to the delivery of phytopharmaceuticals, the main properties of different types of plant drugs, and gives a critical review of properties and limitations connected to the application of numerous lipid, polymer, and inorganic nanoparticles as phytodrug carriers. It also provides information about up-to-date investigated combinations of phytopharmaceuticals and nanocarriers.",
publisher = "Singapore : Springer",
journal = "Role of Herbal Medicines",
booktitle = "Nanotechnology in Delivery and Targeting of Phytochemicals for Lifestyle Diseases",
pages = "524-497",
doi = "10.1007/978-981-99-7703-1_25"
}

Milivojević, M., Pajić-Lijaković, I., Dajić, Z., Dhara, A. K., Nayak, A. K.,& Hasnain, M. S.. (2024). Nanotechnology in Delivery and Targeting of Phytochemicals for Lifestyle Diseases. in Role of Herbal Medicines
Singapore : Springer., 497-524.
https://doi.org/10.1007/978-981-99-7703-1_25

Milivojević M, Pajić-Lijaković I, Dajić Z, Dhara AK, Nayak AK, Hasnain MS. Nanotechnology in Delivery and Targeting of Phytochemicals for Lifestyle Diseases. in Role of Herbal Medicines. 2024;:497-524.
doi:10.1007/978-981-99-7703-1_25 .

Milivojević, Milan, Pajić-Lijaković, Ivana, Dajić, Zora, Dhara, Amal Kumar, Nayak, Amit Kumar, Hasnain, Md Saquib, "Nanotechnology in Delivery and Targeting of Phytochemicals for Lifestyle Diseases" in Role of Herbal Medicines (2024):497-524,
https://doi.org/10.1007/978-981-99-7703-1_25 . .

TY  - CHAP
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7191
AB  - Breast cancer is females’ most common cancer, with a high mortality rate primarily due to metastasis to secondary sites in the body. The migration of cancer cells from the primary tumor is influenced by physical interactions between cancer cells and surrounding epithelium and the extracellular matrix. Cumulative effects of these interactions arise in the form of physical factors such as solid stress accumulated within a tumor spheroid, tissue surface tension, and the viscoelasticity caused by collective cell migration. The cancer spreading is regulated by solid stress generated in spheroid core region during tumor growth and its interactions with external tissue. Tissue viscoelasticity and surface tension are influenced by strength of cell–cell and cell–extracellular matrix adhesion contacts, intracellular signaling cascades, viscoelasticity of extracellular matrix, and cell contractility in response to microenvironmental conditions. However, the interplay between those factors is still unclear. In order to clarify this issue, it is necessary to consider and compare the rearrangement of various mono-cultured breast cancer and epithelial model systems under in vitro conditions like rearrangement of cell spheroids and the fusion of two cell spheroids. In this chapter, focus is on the multi-scale modelling approaches aimed at reproducing and understanding these biological systems.
PB  - London : World Scientific Publishing
T2  - Modeling and Computational Approaches for Multiscale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment
T1  - Modeling and Computational Approaches for Multi-scale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment Viscoelastic Aspects of Solid Cancers
EP  - 119
SP  - 93
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7191
ER  - 

@inbook{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2024",
abstract = "Breast cancer is females’ most common cancer, with a high mortality rate primarily due to metastasis to secondary sites in the body. The migration of cancer cells from the primary tumor is influenced by physical interactions between cancer cells and surrounding epithelium and the extracellular matrix. Cumulative effects of these interactions arise in the form of physical factors such as solid stress accumulated within a tumor spheroid, tissue surface tension, and the viscoelasticity caused by collective cell migration. The cancer spreading is regulated by solid stress generated in spheroid core region during tumor growth and its interactions with external tissue. Tissue viscoelasticity and surface tension are influenced by strength of cell–cell and cell–extracellular matrix adhesion contacts, intracellular signaling cascades, viscoelasticity of extracellular matrix, and cell contractility in response to microenvironmental conditions. However, the interplay between those factors is still unclear. In order to clarify this issue, it is necessary to consider and compare the rearrangement of various mono-cultured breast cancer and epithelial model systems under in vitro conditions like rearrangement of cell spheroids and the fusion of two cell spheroids. In this chapter, focus is on the multi-scale modelling approaches aimed at reproducing and understanding these biological systems.",
publisher = "London : World Scientific Publishing",
journal = "Modeling and Computational Approaches for Multiscale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment",
booktitle = "Modeling and Computational Approaches for Multi-scale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment Viscoelastic Aspects of Solid Cancers",
pages = "119-93",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7191"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2024). Modeling and Computational Approaches for Multi-scale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment Viscoelastic Aspects of Solid Cancers. in Modeling and Computational Approaches for Multiscale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment
London : World Scientific Publishing., 93-119.
https://hdl.handle.net/21.15107/rcub_technorep_7191

Pajić-Lijaković I, Milivojević M. Modeling and Computational Approaches for Multi-scale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment Viscoelastic Aspects of Solid Cancers. in Modeling and Computational Approaches for Multiscale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment. 2024;:93-119.
https://hdl.handle.net/21.15107/rcub_technorep_7191 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Modeling and Computational Approaches for Multi-scale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment Viscoelastic Aspects of Solid Cancers" in Modeling and Computational Approaches for Multiscale Phenomena in Cancer Research: From Cancer Evolution to Cancer Treatment (2024):93-119,
https://hdl.handle.net/21.15107/rcub_technorep_7191 .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5287
AB  - A key feature that distinguishes cancer cells from all other cells is their capability to spread throughout the body. Although how cancer cells collectively migrate by following molecular rules which influence the state of cell-cell adhesion contacts has been comprehensively formulated, the impact of physical interactions on cell spreading remains less understood. Cumulative effects of physical interactions exist as the interplay between various physical parameters such as (1) tissue surface tension, (2) viscoelasticity caused by collective cell migration, and (3) solid stress accumulated in the cell aggregate core region. This review aims to point out the role of these physical parameters in cancer cell spreading by considering and comparing the rearrangement of various mono-cultured cancer and epithelial model systems such as the fusion of two cell aggregates. While epithelial cells undergo volumetric cell rearrangement driven by the tissue surface tension, which directs cell movement from the surface to the core region of two-aggregate systems, cancer cells rather perform surface cell rearrangement. Cancer cells migrate toward the surface of the two-aggregate system driven by the solid stress while the surface tension is significantly reduced. The solid stress, accumulated in the core region of the two-aggregate system, is capable of suppressing the movement of epithelial cells that can undergo the jamming state transition; however, this stress enhances the movement of cancer cells. We have focused here on the multi-scale rheological modeling approaches that aimed at reproducing and understanding these biological systems.
PB  - Tech Science Press
T2  - Biocell
T1  - Surface activity of cancer cells: The fusion of two cell aggregates
EP  - 25
IS  - 1
SP  - 15
VL  - 47
DO  - 10.32604/biocell.2023.023469
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2023",
abstract = "A key feature that distinguishes cancer cells from all other cells is their capability to spread throughout the body. Although how cancer cells collectively migrate by following molecular rules which influence the state of cell-cell adhesion contacts has been comprehensively formulated, the impact of physical interactions on cell spreading remains less understood. Cumulative effects of physical interactions exist as the interplay between various physical parameters such as (1) tissue surface tension, (2) viscoelasticity caused by collective cell migration, and (3) solid stress accumulated in the cell aggregate core region. This review aims to point out the role of these physical parameters in cancer cell spreading by considering and comparing the rearrangement of various mono-cultured cancer and epithelial model systems such as the fusion of two cell aggregates. While epithelial cells undergo volumetric cell rearrangement driven by the tissue surface tension, which directs cell movement from the surface to the core region of two-aggregate systems, cancer cells rather perform surface cell rearrangement. Cancer cells migrate toward the surface of the two-aggregate system driven by the solid stress while the surface tension is significantly reduced. The solid stress, accumulated in the core region of the two-aggregate system, is capable of suppressing the movement of epithelial cells that can undergo the jamming state transition; however, this stress enhances the movement of cancer cells. We have focused here on the multi-scale rheological modeling approaches that aimed at reproducing and understanding these biological systems.",
publisher = "Tech Science Press",
journal = "Biocell",
title = "Surface activity of cancer cells: The fusion of two cell aggregates",
pages = "25-15",
number = "1",
volume = "47",
doi = "10.32604/biocell.2023.023469"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2023). Surface activity of cancer cells: The fusion of two cell aggregates. in Biocell
Tech Science Press., 47(1), 15-25.
https://doi.org/10.32604/biocell.2023.023469

Pajić-Lijaković I, Milivojević M. Surface activity of cancer cells: The fusion of two cell aggregates. in Biocell. 2023;47(1):15-25.
doi:10.32604/biocell.2023.023469 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Surface activity of cancer cells: The fusion of two cell aggregates" in Biocell, 47, no. 1 (2023):15-25,
https://doi.org/10.32604/biocell.2023.023469 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5300
AB  - Morphogenesis, tissue regeneration, and cancer invasion involve transitions in tissue morphology. These transitions, caused by collective cell migration (CCM), have been interpreted as active wetting/de-wetting transitions. This phenomenon is considered based on a model system as wetting of a cell aggregate on a rigid substrate, which includes cell aggregate movement and isotropic/anisotropic spreading of a cell monolayer around the aggregate depending on the substrate rigidity and aggregate size. This model system accounts for the transition between 3D epithelial aggregate and 2D cell monolayer as a product of: (1) tissue surface tension, (2) surface tension of substrate matrix, (3) cell–matrix interfacial tension, (4) interfacial tension gradient, (5) viscoelasticity caused by CCM, and (6) viscoelasticity of substrate matrix. These physical parameters depend on the cell contractility and state of cell–cell and cell–matrix adhesion contacts, as well as the stretching/compression of cellular systems caused by CCM. Despite extensive research devoted to study cell wetting, we still do not understand the interplay among these physical parameters which induces an oscillatory trend of cell rearrangement. This review focuses on these physical parameters in governing the cell rearrangement in the context of epithelial aggregate wetting/de-wetting, and on modeling approaches aimed at reproducing and understanding these biological systems. In this context, we not only review previously published biophysical models for cell rearrangement caused by CCM, but also propose new extensions of those models to point out the interrelation between cell–matrix interfacial tension and epithelial viscoelasticity and the role of the interfacial tension gradient in cell spreading.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - European Biophysics Journal
T1  - Active wetting of epithelial tissues: modeling considerations
EP  - 15
IS  - 1-2
SP  - 1
VL  - 52
DO  - 10.1007/s00249-022-01625-w
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2023",
abstract = "Morphogenesis, tissue regeneration, and cancer invasion involve transitions in tissue morphology. These transitions, caused by collective cell migration (CCM), have been interpreted as active wetting/de-wetting transitions. This phenomenon is considered based on a model system as wetting of a cell aggregate on a rigid substrate, which includes cell aggregate movement and isotropic/anisotropic spreading of a cell monolayer around the aggregate depending on the substrate rigidity and aggregate size. This model system accounts for the transition between 3D epithelial aggregate and 2D cell monolayer as a product of: (1) tissue surface tension, (2) surface tension of substrate matrix, (3) cell–matrix interfacial tension, (4) interfacial tension gradient, (5) viscoelasticity caused by CCM, and (6) viscoelasticity of substrate matrix. These physical parameters depend on the cell contractility and state of cell–cell and cell–matrix adhesion contacts, as well as the stretching/compression of cellular systems caused by CCM. Despite extensive research devoted to study cell wetting, we still do not understand the interplay among these physical parameters which induces an oscillatory trend of cell rearrangement. This review focuses on these physical parameters in governing the cell rearrangement in the context of epithelial aggregate wetting/de-wetting, and on modeling approaches aimed at reproducing and understanding these biological systems. In this context, we not only review previously published biophysical models for cell rearrangement caused by CCM, but also propose new extensions of those models to point out the interrelation between cell–matrix interfacial tension and epithelial viscoelasticity and the role of the interfacial tension gradient in cell spreading.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "European Biophysics Journal",
title = "Active wetting of epithelial tissues: modeling considerations",
pages = "15-1",
number = "1-2",
volume = "52",
doi = "10.1007/s00249-022-01625-w"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2023). Active wetting of epithelial tissues: modeling considerations. in European Biophysics Journal
Springer Science and Business Media Deutschland GmbH., 52(1-2), 1-15.
https://doi.org/10.1007/s00249-022-01625-w

Pajić-Lijaković I, Milivojević M. Active wetting of epithelial tissues: modeling considerations. in European Biophysics Journal. 2023;52(1-2):1-15.
doi:10.1007/s00249-022-01625-w .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Active wetting of epithelial tissues: modeling considerations" in European Biophysics Journal, 52, no. 1-2 (2023):1-15,
https://doi.org/10.1007/s00249-022-01625-w . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6582
AB  - Epithelial cancers are the most common cancer diseases worldwide within last few years. Many of these diseases can be cured if treated effectively in an early stage. The early stage of diseases includes the spreading of cancer cells through healthy epithelium. This spreading of cancer (mesenchymal) cells influences the reorganization of epithelium itself via collective cell migration, which has a feedback to heterotypic interactions along the bio-interface between epithelial and cancer subpopulations and further cancer spreading. This cause–consequence inter-relation is guided by the interplay among biological and physical factors. While biological factors, such as cell signaling and gene expression, which influence cell contractility and remodeling of cell–cell and cell–matrix adhesion contacts and consequently, homotopic and heterotypic interactions are well elaborated, we still don’t understand properly the impact of physical factors on altered morphological changes of epithelial cells. The deeper insight into these physical interactions may help us to solve some long-standing questions in disease progression and can lead to advance in cancer diagnostics and therapies. This review focuses on the role of the physical factors, such as epithelial and cancer surface tensions, interfacial tension between the subpopulations, gradients of interfacial tension of the subpopulations, cell residual stress generation, and frictional effects along the bio-interface on the morphological changes of epithelial cells and the impact of these changes on further spreading of cancer.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Applied Physics A: Materials Science and Processing
T1  - Morphological changes of epithelial cells and spreading of cancer: theoretical consideration
IS  - 8
SP  - 553
VL  - 129
DO  - 10.1007/s00339-023-06814-8
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2023",
abstract = "Epithelial cancers are the most common cancer diseases worldwide within last few years. Many of these diseases can be cured if treated effectively in an early stage. The early stage of diseases includes the spreading of cancer cells through healthy epithelium. This spreading of cancer (mesenchymal) cells influences the reorganization of epithelium itself via collective cell migration, which has a feedback to heterotypic interactions along the bio-interface between epithelial and cancer subpopulations and further cancer spreading. This cause–consequence inter-relation is guided by the interplay among biological and physical factors. While biological factors, such as cell signaling and gene expression, which influence cell contractility and remodeling of cell–cell and cell–matrix adhesion contacts and consequently, homotopic and heterotypic interactions are well elaborated, we still don’t understand properly the impact of physical factors on altered morphological changes of epithelial cells. The deeper insight into these physical interactions may help us to solve some long-standing questions in disease progression and can lead to advance in cancer diagnostics and therapies. This review focuses on the role of the physical factors, such as epithelial and cancer surface tensions, interfacial tension between the subpopulations, gradients of interfacial tension of the subpopulations, cell residual stress generation, and frictional effects along the bio-interface on the morphological changes of epithelial cells and the impact of these changes on further spreading of cancer.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Applied Physics A: Materials Science and Processing",
title = "Morphological changes of epithelial cells and spreading of cancer: theoretical consideration",
number = "8",
pages = "553",
volume = "129",
doi = "10.1007/s00339-023-06814-8"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2023). Morphological changes of epithelial cells and spreading of cancer: theoretical consideration. in Applied Physics A: Materials Science and Processing
Springer Science and Business Media Deutschland GmbH., 129(8), 553.
https://doi.org/10.1007/s00339-023-06814-8

Pajić-Lijaković I, Milivojević M. Morphological changes of epithelial cells and spreading of cancer: theoretical consideration. in Applied Physics A: Materials Science and Processing. 2023;129(8):553.
doi:10.1007/s00339-023-06814-8 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Morphological changes of epithelial cells and spreading of cancer: theoretical consideration" in Applied Physics A: Materials Science and Processing, 129, no. 8 (2023):553,
https://doi.org/10.1007/s00339-023-06814-8 . .

TY  - JOUR
AU  - Milivojević, Milan
AU  - Popović, Aleksandra
AU  - Pajić-Lijaković, Ivana
AU  - Šoštarić, Ivan
AU  - Kolašinac, Stefan
AU  - Dajić Stevanović, Zora
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6616
AB  - Sodium alginate is one of the most interesting and the most investigated and applied biopolymers due to its advantageous properties. Among them, easy, simple, mild, rapid, non-toxic gelation by divalent cations is the most important. In addition, it is abundant, low-cost, eco-friendly, bio-compatible, bio-adhesive, biodegradable, stable, etc. All those properties were systematically considered within this review. Carotenoids are functional components in the human diet with plenty of health benefits. However, their sensitivity to environmental and process stresses, chemical instability, easy oxidation, low water solubility, and bioavailability limit their food and pharmaceutical applications. Encapsulation may help in overcoming these limitations and within this review, the role of alginate-based encapsulation systems in improving the stability and bioavailability of carotenoids is explored. It may be concluded that all alginate-based systems increase carotenoid stability, but only those of micro- and nano-size, as well as emulsion-based, may improve their low bioaccessibility. In addition, the incorporation of other biopolymers may further improve encapsulation system properties. Furthermore, the main techniques for evaluating the encapsulation are briefly considered. This review critically and profoundly explains the role of alginates in improving the encapsulation process of carotenoids, suggesting the best alternatives for those systems. Moreover, it provides a comprehensive cover of recent advances in this field.
PB  - MDPI
T2  - Gels
T1  - Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications
IS  - 8
SP  - 620
VL  - 9
DO  - 10.3390/gels9080620
ER  - 

@article{
author = "Milivojević, Milan and Popović, Aleksandra and Pajić-Lijaković, Ivana and Šoštarić, Ivan and Kolašinac, Stefan and Dajić Stevanović, Zora",
year = "2023",
abstract = "Sodium alginate is one of the most interesting and the most investigated and applied biopolymers due to its advantageous properties. Among them, easy, simple, mild, rapid, non-toxic gelation by divalent cations is the most important. In addition, it is abundant, low-cost, eco-friendly, bio-compatible, bio-adhesive, biodegradable, stable, etc. All those properties were systematically considered within this review. Carotenoids are functional components in the human diet with plenty of health benefits. However, their sensitivity to environmental and process stresses, chemical instability, easy oxidation, low water solubility, and bioavailability limit their food and pharmaceutical applications. Encapsulation may help in overcoming these limitations and within this review, the role of alginate-based encapsulation systems in improving the stability and bioavailability of carotenoids is explored. It may be concluded that all alginate-based systems increase carotenoid stability, but only those of micro- and nano-size, as well as emulsion-based, may improve their low bioaccessibility. In addition, the incorporation of other biopolymers may further improve encapsulation system properties. Furthermore, the main techniques for evaluating the encapsulation are briefly considered. This review critically and profoundly explains the role of alginates in improving the encapsulation process of carotenoids, suggesting the best alternatives for those systems. Moreover, it provides a comprehensive cover of recent advances in this field.",
publisher = "MDPI",
journal = "Gels",
title = "Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications",
number = "8",
pages = "620",
volume = "9",
doi = "10.3390/gels9080620"
}

Milivojević, M., Popović, A., Pajić-Lijaković, I., Šoštarić, I., Kolašinac, S.,& Dajić Stevanović, Z.. (2023). Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications. in Gels
MDPI., 9(8), 620.
https://doi.org/10.3390/gels9080620

Milivojević M, Popović A, Pajić-Lijaković I, Šoštarić I, Kolašinac S, Dajić Stevanović Z. Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications. in Gels. 2023;9(8):620.
doi:10.3390/gels9080620 .

Milivojević, Milan, Popović, Aleksandra, Pajić-Lijaković, Ivana, Šoštarić, Ivan, Kolašinac, Stefan, Dajić Stevanović, Zora, "Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications" in Gels, 9, no. 8 (2023):620,
https://doi.org/10.3390/gels9080620 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6637
AB  - Movement of cell clusters along extracellular matrices (ECM) during tissue development, wound healing, and early stage of cancer invasion involve various inter-connected migration modes such as: (1) cell movement within clusters, (2) cluster extension (wetting) and compression (de-wetting), and (3) directional cluster movement. It has become increasingly evident that dilational and volumetric viscoelasticity of cell clusters and their surrounding substrate significantly influence these migration modes through physical parameters such as: tissue and matrix surface tensions, interfacial tension between cells and substrate, gradients of surface and interfacial tensions, as well as, the accumulation of cell and matrix residual stresses. Inhomogeneous distribution of tissue surface tension along the cell–matrix biointerface can appear as a consequence of different contractility of various cluster regions. While the directional cell migration caused by the matrix stiffness gradient (i.e., durotaxis) has been widely elaborated, the structural changes of matrix surface caused by cell tractions which lead to the generation of the matrix surface tension gradient has not been considered yet. The main goal of this theoretical consideration is to clarify the roles of various physical parameters in collective cell migration based on the formulation of a biophysical model. This complex phenomenon is discussed with the help of model systems such as the movement of cell clusters on a collagen I gel matrix, simultaneously reviewing various experimental data with and without cells.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - European Biophysics Journal
T1  - Physics of collective cell migration
DO  - 10.1007/s00249-023-01681-w
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2023",
abstract = "Movement of cell clusters along extracellular matrices (ECM) during tissue development, wound healing, and early stage of cancer invasion involve various inter-connected migration modes such as: (1) cell movement within clusters, (2) cluster extension (wetting) and compression (de-wetting), and (3) directional cluster movement. It has become increasingly evident that dilational and volumetric viscoelasticity of cell clusters and their surrounding substrate significantly influence these migration modes through physical parameters such as: tissue and matrix surface tensions, interfacial tension between cells and substrate, gradients of surface and interfacial tensions, as well as, the accumulation of cell and matrix residual stresses. Inhomogeneous distribution of tissue surface tension along the cell–matrix biointerface can appear as a consequence of different contractility of various cluster regions. While the directional cell migration caused by the matrix stiffness gradient (i.e., durotaxis) has been widely elaborated, the structural changes of matrix surface caused by cell tractions which lead to the generation of the matrix surface tension gradient has not been considered yet. The main goal of this theoretical consideration is to clarify the roles of various physical parameters in collective cell migration based on the formulation of a biophysical model. This complex phenomenon is discussed with the help of model systems such as the movement of cell clusters on a collagen I gel matrix, simultaneously reviewing various experimental data with and without cells.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "European Biophysics Journal",
title = "Physics of collective cell migration",
doi = "10.1007/s00249-023-01681-w"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2023). Physics of collective cell migration. in European Biophysics Journal
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s00249-023-01681-w

Pajić-Lijaković I, Milivojević M. Physics of collective cell migration. in European Biophysics Journal. 2023;.
doi:10.1007/s00249-023-01681-w .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Physics of collective cell migration" in European Biophysics Journal (2023),
https://doi.org/10.1007/s00249-023-01681-w . .

TY  - JOUR
AU  - Espina, Jaime A.
AU  - Cordeiro, Marilia H.
AU  - Milivojević, Milan
AU  - Pajić-Lijaković, Ivana
AU  - Barriga, Elias H.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6662
AB  - Shear stress is essential for normal physiology and malignancy. Common physiological processes - such as blood flow, particle flow in the gut, or contact between migratory cell clusters and their substrate - produce shear stress that can have an impact on the behavior of different tissues. In addition, shear stress has roles in processes of biomedical interest, such as wound healing, cancer and fibrosis induced by soft implants. Thus, understanding how cells react and adapt to shear stress is important. In this Review, we discuss in vivo and in vitro data obtained from vascular and epithelial models; highlight the insights these have afforded regarding the general mechanisms through which cells sense, transduce and respond to shear stress at the cellular levels; and outline how the changes cells experience in response to shear stress impact tissue organization. Finally, we discuss the role of shear stress in collective cell migration, which is only starting to be appreciated. We review our current understanding of the effects of shear stress in the context of embryo development, cancer and fibrosis, and invite the scientific community to further investigate the role of shear stress in these scenarios.
PB  - Company of Biologists Ltd.
T2  - Journal of Cell Science
T1  - Response of cells and tissues to shear stress
IS  - 18
SP  - jcs260985
VL  - 136
DO  - 10.1242/jcs.260985
ER  - 

@article{
author = "Espina, Jaime A. and Cordeiro, Marilia H. and Milivojević, Milan and Pajić-Lijaković, Ivana and Barriga, Elias H.",
year = "2023",
abstract = "Shear stress is essential for normal physiology and malignancy. Common physiological processes - such as blood flow, particle flow in the gut, or contact between migratory cell clusters and their substrate - produce shear stress that can have an impact on the behavior of different tissues. In addition, shear stress has roles in processes of biomedical interest, such as wound healing, cancer and fibrosis induced by soft implants. Thus, understanding how cells react and adapt to shear stress is important. In this Review, we discuss in vivo and in vitro data obtained from vascular and epithelial models; highlight the insights these have afforded regarding the general mechanisms through which cells sense, transduce and respond to shear stress at the cellular levels; and outline how the changes cells experience in response to shear stress impact tissue organization. Finally, we discuss the role of shear stress in collective cell migration, which is only starting to be appreciated. We review our current understanding of the effects of shear stress in the context of embryo development, cancer and fibrosis, and invite the scientific community to further investigate the role of shear stress in these scenarios.",
publisher = "Company of Biologists Ltd.",
journal = "Journal of Cell Science",
title = "Response of cells and tissues to shear stress",
number = "18",
pages = "jcs260985",
volume = "136",
doi = "10.1242/jcs.260985"
}

Espina, J. A., Cordeiro, M. H., Milivojević, M., Pajić-Lijaković, I.,& Barriga, E. H.. (2023). Response of cells and tissues to shear stress. in Journal of Cell Science
Company of Biologists Ltd.., 136(18), jcs260985.
https://doi.org/10.1242/jcs.260985

Espina JA, Cordeiro MH, Milivojević M, Pajić-Lijaković I, Barriga EH. Response of cells and tissues to shear stress. in Journal of Cell Science. 2023;136(18):jcs260985.
doi:10.1242/jcs.260985 .

Espina, Jaime A., Cordeiro, Marilia H., Milivojević, Milan, Pajić-Lijaković, Ivana, Barriga, Elias H., "Response of cells and tissues to shear stress" in Journal of Cell Science, 136, no. 18 (2023):jcs260985,
https://doi.org/10.1242/jcs.260985 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6711
AB  - Collective cell migration is essential for a wide range of biological processes such as: morphogenesis, wound healing, and cancer spreading. However, it is well known that migrating epithelial collectives frequently undergo jamming, stay trapped some period of time, and then start migration again. Consequently, only a part of epithelial cells actively contributes to the tissue development. In contrast to epithelial cells, migrating mesenchymal collectives successfully avoid the jamming. It has been confirmed that the epithelial unjamming cannot be treated as the epithelial-to-mesenchymal transition. Some other mechanism is responsible for the epithelial jamming/unjamming. Despite extensive research devoted to study the cell jamming/unjamming, we still do not understand the origin of this phenomenon. The origin is connected to physical factors such as: the cell compressive residual stress accumulation and surface characteristics of migrating (unjamming) and resting (jamming) epithelial clusters which depend primarily on the strength of cell-cell adhesion contacts and cell contractility. The main goal of this theoretical consideration is to clarify these cause-consequence relations.
PB  - Elsevier Ltd.
T2  - BioSystems
T1  - Cell jamming-to-unjamming transitions and vice versa in development: Physical aspects
SP  - 105045
VL  - 234
DO  - 10.1016/j.biosystems.2023.105045
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2023",
abstract = "Collective cell migration is essential for a wide range of biological processes such as: morphogenesis, wound healing, and cancer spreading. However, it is well known that migrating epithelial collectives frequently undergo jamming, stay trapped some period of time, and then start migration again. Consequently, only a part of epithelial cells actively contributes to the tissue development. In contrast to epithelial cells, migrating mesenchymal collectives successfully avoid the jamming. It has been confirmed that the epithelial unjamming cannot be treated as the epithelial-to-mesenchymal transition. Some other mechanism is responsible for the epithelial jamming/unjamming. Despite extensive research devoted to study the cell jamming/unjamming, we still do not understand the origin of this phenomenon. The origin is connected to physical factors such as: the cell compressive residual stress accumulation and surface characteristics of migrating (unjamming) and resting (jamming) epithelial clusters which depend primarily on the strength of cell-cell adhesion contacts and cell contractility. The main goal of this theoretical consideration is to clarify these cause-consequence relations.",
publisher = "Elsevier Ltd.",
journal = "BioSystems",
title = "Cell jamming-to-unjamming transitions and vice versa in development: Physical aspects",
pages = "105045",
volume = "234",
doi = "10.1016/j.biosystems.2023.105045"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2023). Cell jamming-to-unjamming transitions and vice versa in development: Physical aspects. in BioSystems
Elsevier Ltd.., 234, 105045.
https://doi.org/10.1016/j.biosystems.2023.105045

Pajić-Lijaković I, Milivojević M. Cell jamming-to-unjamming transitions and vice versa in development: Physical aspects. in BioSystems. 2023;234:105045.
doi:10.1016/j.biosystems.2023.105045 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Cell jamming-to-unjamming transitions and vice versa in development: Physical aspects" in BioSystems, 234 (2023):105045,
https://doi.org/10.1016/j.biosystems.2023.105045 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7042
AB  - The biointerface dynamics influence any cancer spreading through the epithelium since it is documented in the early stages some malignancies (like epithelial cancer). The altered rearrangement of epithelial cells has an impact on the development of cancer. Therefore, it is necessary to comprehend the underlying biological and physical mechanisms of this biointerface dynamics for early suppression of cancer. While the biological mechanisms include cell signaling and gene expression, the physical mechanisms are several physical parameters such as the epithelial-cancer interfacial tension, epithelial surface tension, and compressive stress accumulated within the epithelium. Although the segregation of epithelia-cancer co-cultured systems was widely investigated, the role of these physical parameters in cell reorganization is still not fully recognized. Hence, this review is focused on clarifying the role that some physical parameters have during cell reorganization within the epithelial cell clusters and cancer spread within co-cultured spheroids. We have applied the developed biophysical model to point out the inter-relations among physical parameters that influence cell reorganization within epithelial-cancer co-cultured systems. The main results of this theoretical consideration have been assessed by integrating the biophysical model with biological and bio-mechanical experiments from the available literature. The epithelial-cancer interfacial tension leads to the reduction of the biointerface area, which leads to an increase in the compressive residual stress within the epithelial clusters depending on the viscoelasticity of the epithelial subpopulation. This stress impacts epithelial rearrangement and the dynamics along the biointerface by influencing the epithelial surface tension and epithelial-cancer interfacial tension. Further, the interrelation between the epithelial surface tension and epithelial-cancer interfacial tension influences the spread of cancer cells.
PB  - Tech Science Press
T2  - Biocell
T1  - Dynamics along the epithelial-cancer biointerface: Hidden system complexities
EP  - 2334
IS  - 11
SP  - 2321
VL  - 47
DO  - 10.32604/biocell.2023.043796
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2023",
abstract = "The biointerface dynamics influence any cancer spreading through the epithelium since it is documented in the early stages some malignancies (like epithelial cancer). The altered rearrangement of epithelial cells has an impact on the development of cancer. Therefore, it is necessary to comprehend the underlying biological and physical mechanisms of this biointerface dynamics for early suppression of cancer. While the biological mechanisms include cell signaling and gene expression, the physical mechanisms are several physical parameters such as the epithelial-cancer interfacial tension, epithelial surface tension, and compressive stress accumulated within the epithelium. Although the segregation of epithelia-cancer co-cultured systems was widely investigated, the role of these physical parameters in cell reorganization is still not fully recognized. Hence, this review is focused on clarifying the role that some physical parameters have during cell reorganization within the epithelial cell clusters and cancer spread within co-cultured spheroids. We have applied the developed biophysical model to point out the inter-relations among physical parameters that influence cell reorganization within epithelial-cancer co-cultured systems. The main results of this theoretical consideration have been assessed by integrating the biophysical model with biological and bio-mechanical experiments from the available literature. The epithelial-cancer interfacial tension leads to the reduction of the biointerface area, which leads to an increase in the compressive residual stress within the epithelial clusters depending on the viscoelasticity of the epithelial subpopulation. This stress impacts epithelial rearrangement and the dynamics along the biointerface by influencing the epithelial surface tension and epithelial-cancer interfacial tension. Further, the interrelation between the epithelial surface tension and epithelial-cancer interfacial tension influences the spread of cancer cells.",
publisher = "Tech Science Press",
journal = "Biocell",
title = "Dynamics along the epithelial-cancer biointerface: Hidden system complexities",
pages = "2334-2321",
number = "11",
volume = "47",
doi = "10.32604/biocell.2023.043796"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2023). Dynamics along the epithelial-cancer biointerface: Hidden system complexities. in Biocell
Tech Science Press., 47(11), 2321-2334.
https://doi.org/10.32604/biocell.2023.043796

Pajić-Lijaković I, Milivojević M. Dynamics along the epithelial-cancer biointerface: Hidden system complexities. in Biocell. 2023;47(11):2321-2334.
doi:10.32604/biocell.2023.043796 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Dynamics along the epithelial-cancer biointerface: Hidden system complexities" in Biocell, 47, no. 11 (2023):2321-2334,
https://doi.org/10.32604/biocell.2023.043796 . .

TY  - JOUR
AU  - Veljković, Milica
AU  - Stepanović, Relja
AU  - Banjanac, Katarina
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Milivojević, Milan
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5247
AB  - Within this study, the investigation of potential selective immobilization of fructosyltransferase (FTase) derived from Aspergillus aculeatus (from commercial enzyme preparation Pectinex® Ultra SP-L) onto the polystyrenic macroporous resin Purolite® A109 and its further utilization for the synthesis of a highly valuable group of prebiotic compounds named fructo-oligosaccharides (FOS), was performed. The obtained results suggest that partial purification of fructosyltransferase from complex enzyme mixture with predominant pectinase activity (ratio of activity immobilization yields around 20.0) occurred by performing a simple immobilization protocol, provided that immobilization lasted for 5 h using an enzyme concentration of 50 mg g−1 of support under slightly acidic conditions (pH 6.0). Immobilized preparation was thereafter successfully utilized for FOS synthesis (56–59 % FOS of total sugars) in both a batch and air-lift reactor system. Operational stability study proved the exceptionally high potential of immobilized preparation for reproducible and cost-effective application, since 90 % of initial activity was preserved after ten consecutive reaction cycles. Thereafter, a prospectus for continuous production of FOS in the air-lift reactor was assayed. Achieved yields, 53 % FOS of total sugars for at least 9 h, indicated the great suitability of the obtained immobilized preparation for application in large-scale systems.
PB  - Korean Society of Industrial Engineering Chemistry
T2  - Journal of Industrial and Engineering Chemistry
T1  - Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109
EP  - 156
SP  - 149
VL  - 117
DO  - 10.1016/j.jiec.2022.09.051
ER  - 

@article{
author = "Veljković, Milica and Stepanović, Relja and Banjanac, Katarina and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Milivojević, Milan and Bezbradica, Dejan",
year = "2023",
abstract = "Within this study, the investigation of potential selective immobilization of fructosyltransferase (FTase) derived from Aspergillus aculeatus (from commercial enzyme preparation Pectinex® Ultra SP-L) onto the polystyrenic macroporous resin Purolite® A109 and its further utilization for the synthesis of a highly valuable group of prebiotic compounds named fructo-oligosaccharides (FOS), was performed. The obtained results suggest that partial purification of fructosyltransferase from complex enzyme mixture with predominant pectinase activity (ratio of activity immobilization yields around 20.0) occurred by performing a simple immobilization protocol, provided that immobilization lasted for 5 h using an enzyme concentration of 50 mg g−1 of support under slightly acidic conditions (pH 6.0). Immobilized preparation was thereafter successfully utilized for FOS synthesis (56–59 % FOS of total sugars) in both a batch and air-lift reactor system. Operational stability study proved the exceptionally high potential of immobilized preparation for reproducible and cost-effective application, since 90 % of initial activity was preserved after ten consecutive reaction cycles. Thereafter, a prospectus for continuous production of FOS in the air-lift reactor was assayed. Achieved yields, 53 % FOS of total sugars for at least 9 h, indicated the great suitability of the obtained immobilized preparation for application in large-scale systems.",
publisher = "Korean Society of Industrial Engineering Chemistry",
journal = "Journal of Industrial and Engineering Chemistry",
title = "Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109",
pages = "156-149",
volume = "117",
doi = "10.1016/j.jiec.2022.09.051"
}

Veljković, M., Stepanović, R., Banjanac, K., Ćorović, M., Milivojević, A., Simović, M., Milivojević, M.,& Bezbradica, D.. (2023). Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109. in Journal of Industrial and Engineering Chemistry
Korean Society of Industrial Engineering Chemistry., 117, 149-156.
https://doi.org/10.1016/j.jiec.2022.09.051

Veljković M, Stepanović R, Banjanac K, Ćorović M, Milivojević A, Simović M, Milivojević M, Bezbradica D. Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109. in Journal of Industrial and Engineering Chemistry. 2023;117:149-156.
doi:10.1016/j.jiec.2022.09.051 .

Veljković, Milica, Stepanović, Relja, Banjanac, Katarina, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Milivojević, Milan, Bezbradica, Dejan, "Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109" in Journal of Industrial and Engineering Chemistry, 117 (2023):149-156,
https://doi.org/10.1016/j.jiec.2022.09.051 . .

Avramov-Ivić, M., Gavrovska, A., Vuković, D., Mijin, D., Petrović, S., Reljin, B., Reljin, I., Milivojević, M., Stevanović, S.,& Lović, J.. (2023). Sistem i postupak automatske klasifikacije UV/VIS signala radi dijagnostike bilijarne ciroze i njihova primena. in Katalog / 37. Međunarodna izložba Pronalazaka, novih tehnologija i industrijskog dizajna „Pronalazaštvo-Beograd 2023.”, Beograd, 13-16.06.2023
Beograd : Savez pronalazača Beograda., 95-96.
https://hdl.handle.net/21.15107/rcub_technorep_6971

Avramov-Ivić M, Gavrovska A, Vuković D, Mijin D, Petrović S, Reljin B, Reljin I, Milivojević M, Stevanović S, Lović J. Sistem i postupak automatske klasifikacije UV/VIS signala radi dijagnostike bilijarne ciroze i njihova primena. in Katalog / 37. Međunarodna izložba Pronalazaka, novih tehnologija i industrijskog dizajna „Pronalazaštvo-Beograd 2023.”, Beograd, 13-16.06.2023. 2023;:95-96.
https://hdl.handle.net/21.15107/rcub_technorep_6971 .

Avramov-Ivić, Milka, Gavrovska, Ana, Vuković, Dragan, Mijin, Dušan, Petrović, Slobodan, Reljin, Branimir, Reljin, Irini, Milivojević, Milan, Stevanović, Sanja, Lović, Jelena, "Sistem i postupak automatske klasifikacije UV/VIS signala radi dijagnostike bilijarne ciroze i njihova primena" in Katalog / 37. Međunarodna izložba Pronalazaka, novih tehnologija i industrijskog dizajna „Pronalazaštvo-Beograd 2023.”, Beograd, 13-16.06.2023 (2023):95-96,
https://hdl.handle.net/21.15107/rcub_technorep_6971 .

TY  - JOUR
AU  - Lopičić, Zorica
AU  - Antanasković, Anja
AU  - Šoštarić, Tatjana
AU  - Adamović, Vladimir
AU  - Orlić, Marina
AU  - Milojković, Jelena
AU  - Milivojević, Milan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6606
AB  - Peach stones, a valuable agro-industrial by-product available in many countries worldwide, comprise a renewable resource, which can be widely applied for multifunctional purposes. Its important advantages such as high-energy value, low ash content, low price and wide abundance, make peach stones an ideal fuel for energy production, but also for new materials synthesis. Although peach stones exhibit adequate combustion properties, allowing their direct use with minimal physical/chemical treatment, they often need further modification in order to improve their thermal properties, where slow pyrolysis is frequently used. This study aims to provide a practical and effective solution to the revalorization of waste biomass originating from the fruit processing industry, through slow pyrolysis in order to convert this waste into carbonaceous material - biochar. The thermo-chemical conversion of raw biomass resulted in a stable material with excellent fuel properties, with higher mass energy density and grinding ability, providing biochar with properties, in energy sense, similar or even better than a coal. Biochar has a higher fixed carbon content and a higher energy potential than biomass itself, and its application as a biofuel might reduce emissions of greenhouse gases, as it reduces the amount of waste landed and increases the share of energy generated from renewable sources.
AB  - Koštice breskve, otpadna biomasa poreklom iz industrije prerade voća, dostupna u mnogim zemljama širom sveta, predstavlja obnovljivi resurs koji može imati različitu primenu. Prednosti koje se ogledaju u velikom energetskom potencijalu, malom sadržaju pepela, niskoj ceni i širokoj rasprostranjenosti, čine koštice breskve idealnim resursom za proizvodnju energije, ali i za sintezu novih materijala. Iako koštice breskve imaju svojstva koja im omogućavaju da se koriste uz minimalni fizičkio-hemijski tretman, često im je potrebna dodatna modifikacija kako bi se poboljšala njihova svojstva. Jedan on načina poboljšanja energetskih karakteristika otpadne biomase predstavlja termohemijska konverzija materijala u vidu spore pirolize. Ispitivanja prikazana u ovom radu obavljena su sa ciljem obezbeđivanja praktičnog i efikasnog rešenje za revalorizaciju otpadne biomase koja potiče iz industrije prerade voća, putem spore pirolize kojom se vrši konverzija ovog otpada u ugljenični materijal – biočađ. Prikazani rezultati ukazuju na glavna svojstva dobijene biočađi u funkciji njene primene kao čvrstog goriva, istovremeno ih upoređujući sa svojstvima sirove biomase. Termohemijska konverzija polazne lignocelulozne biomase daje stabilni ugljenični material odličnih gorivih karakteristika, veće energetske gustine i boljih mehaničkih svojstava, što rezultuje energetskim svojstvima koja su bolja od uglja. Biočađ poseduje znatno veći energetski potencijal od same biomase, i njegova primena kao biogoriva može redukovati emisiju gasova sa efektom staklene baste, pri čemu se istovremeno smanjuje količina deponovanog otpada ali i povećava udeo energije generisane iz obnovljivih izvora.
PB  - Association of the Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Improvement of energy properties of lignocellulosic waste by thermochemical conversion into biochar
T1  - Poboljšanje energetskih svojstava lignoceluloznog otpada termohemijskom konverzijom u biočađ
EP  - 153
IS  - 2
SP  - 147
VL  - 77
DO  - 10.2298/HEMIND221222013L
ER  - 

@article{
author = "Lopičić, Zorica and Antanasković, Anja and Šoštarić, Tatjana and Adamović, Vladimir and Orlić, Marina and Milojković, Jelena and Milivojević, Milan",
year = "2023",
abstract = "Peach stones, a valuable agro-industrial by-product available in many countries worldwide, comprise a renewable resource, which can be widely applied for multifunctional purposes. Its important advantages such as high-energy value, low ash content, low price and wide abundance, make peach stones an ideal fuel for energy production, but also for new materials synthesis. Although peach stones exhibit adequate combustion properties, allowing their direct use with minimal physical/chemical treatment, they often need further modification in order to improve their thermal properties, where slow pyrolysis is frequently used. This study aims to provide a practical and effective solution to the revalorization of waste biomass originating from the fruit processing industry, through slow pyrolysis in order to convert this waste into carbonaceous material - biochar. The thermo-chemical conversion of raw biomass resulted in a stable material with excellent fuel properties, with higher mass energy density and grinding ability, providing biochar with properties, in energy sense, similar or even better than a coal. Biochar has a higher fixed carbon content and a higher energy potential than biomass itself, and its application as a biofuel might reduce emissions of greenhouse gases, as it reduces the amount of waste landed and increases the share of energy generated from renewable sources., Koštice breskve, otpadna biomasa poreklom iz industrije prerade voća, dostupna u mnogim zemljama širom sveta, predstavlja obnovljivi resurs koji može imati različitu primenu. Prednosti koje se ogledaju u velikom energetskom potencijalu, malom sadržaju pepela, niskoj ceni i širokoj rasprostranjenosti, čine koštice breskve idealnim resursom za proizvodnju energije, ali i za sintezu novih materijala. Iako koštice breskve imaju svojstva koja im omogućavaju da se koriste uz minimalni fizičkio-hemijski tretman, često im je potrebna dodatna modifikacija kako bi se poboljšala njihova svojstva. Jedan on načina poboljšanja energetskih karakteristika otpadne biomase predstavlja termohemijska konverzija materijala u vidu spore pirolize. Ispitivanja prikazana u ovom radu obavljena su sa ciljem obezbeđivanja praktičnog i efikasnog rešenje za revalorizaciju otpadne biomase koja potiče iz industrije prerade voća, putem spore pirolize kojom se vrši konverzija ovog otpada u ugljenični materijal – biočađ. Prikazani rezultati ukazuju na glavna svojstva dobijene biočađi u funkciji njene primene kao čvrstog goriva, istovremeno ih upoređujući sa svojstvima sirove biomase. Termohemijska konverzija polazne lignocelulozne biomase daje stabilni ugljenični material odličnih gorivih karakteristika, veće energetske gustine i boljih mehaničkih svojstava, što rezultuje energetskim svojstvima koja su bolja od uglja. Biočađ poseduje znatno veći energetski potencijal od same biomase, i njegova primena kao biogoriva može redukovati emisiju gasova sa efektom staklene baste, pri čemu se istovremeno smanjuje količina deponovanog otpada ali i povećava udeo energije generisane iz obnovljivih izvora.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Improvement of energy properties of lignocellulosic waste by thermochemical conversion into biochar, Poboljšanje energetskih svojstava lignoceluloznog otpada termohemijskom konverzijom u biočađ",
pages = "153-147",
number = "2",
volume = "77",
doi = "10.2298/HEMIND221222013L"
}

Lopičić, Z., Antanasković, A., Šoštarić, T., Adamović, V., Orlić, M., Milojković, J.,& Milivojević, M.. (2023). Improvement of energy properties of lignocellulosic waste by thermochemical conversion into biochar. in Hemijska industrija
Association of the Chemical Engineers of Serbia., 77(2), 147-153.
https://doi.org/10.2298/HEMIND221222013L

Lopičić Z, Antanasković A, Šoštarić T, Adamović V, Orlić M, Milojković J, Milivojević M. Improvement of energy properties of lignocellulosic waste by thermochemical conversion into biochar. in Hemijska industrija. 2023;77(2):147-153.
doi:10.2298/HEMIND221222013L .

Lopičić, Zorica, Antanasković, Anja, Šoštarić, Tatjana, Adamović, Vladimir, Orlić, Marina, Milojković, Jelena, Milivojević, Milan, "Improvement of energy properties of lignocellulosic waste by thermochemical conversion into biochar" in Hemijska industrija, 77, no. 2 (2023):147-153,
https://doi.org/10.2298/HEMIND221222013L . .

TY  - JOUR
AU  - Antanasković, Anja
AU  - Lopičić, Zorica
AU  - Pehlivan, Erol
AU  - Adamović, Vladimir
AU  - Šoštarić, Tatjana
AU  - Milojković, Jelena
AU  - Milivojević, Milan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6333
AB  - In this study, slow pyrolysis was employed for the thermochemical conversion of peach stones (PS), lignocellulosic waste (LCW) from food industry, to prepare biochar (PS-B), a new and efficient sorbent for the removal of toxic dye, brilliant green (BG), from aqueous solution. Detailed characteristics of PS and PS-B were analysed using pH suspension, pHPZC, zeta potential, particle size distribution, thermogravimetric method (TG-DTG), scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM–EDX), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The results revealed that material loss during slow pyrolysis not only altered the chemical structure of the samples, but also induced the volume shrinking phenomenon, resulting in production of a rougher, porous structure with decreased crystallinity. Also, five times less total organic carbon (TOC) release was demonstrated by biochar compared to native material. Comparison of experimental results for BG sorption by PS and PS-B showed that sorption capacity increased after pyrolysis and that sorption kinetic was significantly improved. The BG sorption by both sorbents followed the pseudo-second-order reaction kinetic model, with the intraparticle diffusion model within pores controlling the adsorption rate in PS-B. Isotherm equilibrium modelling revealed that the Sips isotherm provided the best model fit with maximum sorption capacity of 82.53 and 101.11 mg/g for PS and PS-B, respectively. A possible sorption mechanism of BG was achieved through pore filling (porous diffusion), hydrogen bonding, electrostatic attraction and π-π interactions. The results demonstrated that biochar derived from peach stones can be used as a promising green material for removing BG from contaminated waters.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Biomass Conversion and Biorefinery
T1  - Thermochemical conversion of non-edible fruit waste for dye removal from wastewater
DO  - 10.1007/s13399-023-04083-2
ER  - 

@article{
author = "Antanasković, Anja and Lopičić, Zorica and Pehlivan, Erol and Adamović, Vladimir and Šoštarić, Tatjana and Milojković, Jelena and Milivojević, Milan",
year = "2023",
abstract = "In this study, slow pyrolysis was employed for the thermochemical conversion of peach stones (PS), lignocellulosic waste (LCW) from food industry, to prepare biochar (PS-B), a new and efficient sorbent for the removal of toxic dye, brilliant green (BG), from aqueous solution. Detailed characteristics of PS and PS-B were analysed using pH suspension, pHPZC, zeta potential, particle size distribution, thermogravimetric method (TG-DTG), scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM–EDX), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The results revealed that material loss during slow pyrolysis not only altered the chemical structure of the samples, but also induced the volume shrinking phenomenon, resulting in production of a rougher, porous structure with decreased crystallinity. Also, five times less total organic carbon (TOC) release was demonstrated by biochar compared to native material. Comparison of experimental results for BG sorption by PS and PS-B showed that sorption capacity increased after pyrolysis and that sorption kinetic was significantly improved. The BG sorption by both sorbents followed the pseudo-second-order reaction kinetic model, with the intraparticle diffusion model within pores controlling the adsorption rate in PS-B. Isotherm equilibrium modelling revealed that the Sips isotherm provided the best model fit with maximum sorption capacity of 82.53 and 101.11 mg/g for PS and PS-B, respectively. A possible sorption mechanism of BG was achieved through pore filling (porous diffusion), hydrogen bonding, electrostatic attraction and π-π interactions. The results demonstrated that biochar derived from peach stones can be used as a promising green material for removing BG from contaminated waters.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Biomass Conversion and Biorefinery",
title = "Thermochemical conversion of non-edible fruit waste for dye removal from wastewater",
doi = "10.1007/s13399-023-04083-2"
}

Antanasković, A., Lopičić, Z., Pehlivan, E., Adamović, V., Šoštarić, T., Milojković, J.,& Milivojević, M.. (2023). Thermochemical conversion of non-edible fruit waste for dye removal from wastewater. in Biomass Conversion and Biorefinery
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s13399-023-04083-2

Antanasković A, Lopičić Z, Pehlivan E, Adamović V, Šoštarić T, Milojković J, Milivojević M. Thermochemical conversion of non-edible fruit waste for dye removal from wastewater. in Biomass Conversion and Biorefinery. 2023;.
doi:10.1007/s13399-023-04083-2 .

Antanasković, Anja, Lopičić, Zorica, Pehlivan, Erol, Adamović, Vladimir, Šoštarić, Tatjana, Milojković, Jelena, Milivojević, Milan, "Thermochemical conversion of non-edible fruit waste for dye removal from wastewater" in Biomass Conversion and Biorefinery (2023),
https://doi.org/10.1007/s13399-023-04083-2 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5164
AB  - Cells are very sensitive to the shear stress (SS). However, undesirable SS is generated during physiological process such as collective cell migration (CCM) and influences the biological processes such as morphogenesis, wound healing and cancer invasion. Despite extensive research devoted to study the SS generation caused by CCM, we still do not fully understand the main cause of SS appearance. An attempt is made here to offer some answers to these questions by considering the rearrangement of cell monolayers. The SS generation represents a consequence of natural and forced convection. While forced convection is dependent on cell speed, the natural convection is induced by the gradient of tissue surface tension. The phenomenon is known as the Marangoni effect. The gradient of tissue surface tension induces directed cell spreading from the regions of lower tissue surface tension to the regions of higher tissue surface tension and leads to the cell sorting. This directional cell migration is described by the Marangoni flux. The phenomenon has been recognized during the rearrangement of (1) epithelial cell monolayers and (2) mixed cell monolayers made by epithelial and mesenchymal cells. The consequence of the Marangoni effect is an intensive spreading of cancer cells through an epithelium. In this work, a review of existing literature about SS generation caused by CCM is given along with the assortment of published experimental findings, to invite experimentalists to test given theoretical considerations in multicellular systems.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - European Biophysics Journal
T1  - Marangoni effect and cell spreading
EP  - 429
IS  - 6
SP  - 419
VL  - 51
DO  - 10.1007/s00249-022-01612-1
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2022",
abstract = "Cells are very sensitive to the shear stress (SS). However, undesirable SS is generated during physiological process such as collective cell migration (CCM) and influences the biological processes such as morphogenesis, wound healing and cancer invasion. Despite extensive research devoted to study the SS generation caused by CCM, we still do not fully understand the main cause of SS appearance. An attempt is made here to offer some answers to these questions by considering the rearrangement of cell monolayers. The SS generation represents a consequence of natural and forced convection. While forced convection is dependent on cell speed, the natural convection is induced by the gradient of tissue surface tension. The phenomenon is known as the Marangoni effect. The gradient of tissue surface tension induces directed cell spreading from the regions of lower tissue surface tension to the regions of higher tissue surface tension and leads to the cell sorting. This directional cell migration is described by the Marangoni flux. The phenomenon has been recognized during the rearrangement of (1) epithelial cell monolayers and (2) mixed cell monolayers made by epithelial and mesenchymal cells. The consequence of the Marangoni effect is an intensive spreading of cancer cells through an epithelium. In this work, a review of existing literature about SS generation caused by CCM is given along with the assortment of published experimental findings, to invite experimentalists to test given theoretical considerations in multicellular systems.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "European Biophysics Journal",
title = "Marangoni effect and cell spreading",
pages = "429-419",
number = "6",
volume = "51",
doi = "10.1007/s00249-022-01612-1"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2022). Marangoni effect and cell spreading. in European Biophysics Journal
Springer Science and Business Media Deutschland GmbH., 51(6), 419-429.
https://doi.org/10.1007/s00249-022-01612-1

Pajić-Lijaković I, Milivojević M. Marangoni effect and cell spreading. in European Biophysics Journal. 2022;51(6):419-429.
doi:10.1007/s00249-022-01612-1 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Marangoni effect and cell spreading" in European Biophysics Journal, 51, no. 6 (2022):419-429,
https://doi.org/10.1007/s00249-022-01612-1 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7170
AB  - Although collective cell migration (CCM) is a highly coordinated and fine-tuned migratory mode, instabilities in the form of cell swirling motion (CSM) often occur. The CSM represents a product of the active turbulence obtained at low Reynolds number which has a feedback impact on various processes such as: morphogenesis, wound healing, and cancer invasion. The cause of this phenomenon is related to the viscoelasticity of multicellular systems in the context of cell residual stress accumulation. Particular interest of this work is to: (1) consider the tissue cohesiveness as the main parameter responsible for the CSM appearance, (2) discuss the viscoelasticity of multicellular systems caused by CCM by clarifying the roles of cell shear and normal residual stresses, and (3) describe the dynamics of CSM in the context of mechanical waves generation based on multiscale modeling consideration. While the cell normal residual stress induces an increase in cell packing density capable of reducing the tissue cohesiveness of healthy epithelium, the shear residual stress exerts work via shear stress torque against the tissue surface tension and can induce the CSM. Inhomogeneous distribution of the cell residual stress within the swirl leads to the generation of viscoelastic force capable of suppressing the CCM. This force together with the surface tension force acts against the centrifugal force and induces the swirl radial pulsations connected with successive stiffening and softening. In this work, a review of existing literature about viscoelasticity caused by CCM is given along with assortment of published experimental findings, in order to invite experimentalists to test given theoretical considerations in multicellular systems.
PB  - Academic Press Inc.
T2  - Advances in Applied Mechanics
T1  - Viscoelasticity and cell swirling motion
EP  - 425
SP  - 393
VL  - 55
DO  - 10.1016/bs.aams.2022.05.002
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2022",
abstract = "Although collective cell migration (CCM) is a highly coordinated and fine-tuned migratory mode, instabilities in the form of cell swirling motion (CSM) often occur. The CSM represents a product of the active turbulence obtained at low Reynolds number which has a feedback impact on various processes such as: morphogenesis, wound healing, and cancer invasion. The cause of this phenomenon is related to the viscoelasticity of multicellular systems in the context of cell residual stress accumulation. Particular interest of this work is to: (1) consider the tissue cohesiveness as the main parameter responsible for the CSM appearance, (2) discuss the viscoelasticity of multicellular systems caused by CCM by clarifying the roles of cell shear and normal residual stresses, and (3) describe the dynamics of CSM in the context of mechanical waves generation based on multiscale modeling consideration. While the cell normal residual stress induces an increase in cell packing density capable of reducing the tissue cohesiveness of healthy epithelium, the shear residual stress exerts work via shear stress torque against the tissue surface tension and can induce the CSM. Inhomogeneous distribution of the cell residual stress within the swirl leads to the generation of viscoelastic force capable of suppressing the CCM. This force together with the surface tension force acts against the centrifugal force and induces the swirl radial pulsations connected with successive stiffening and softening. In this work, a review of existing literature about viscoelasticity caused by CCM is given along with assortment of published experimental findings, in order to invite experimentalists to test given theoretical considerations in multicellular systems.",
publisher = "Academic Press Inc.",
journal = "Advances in Applied Mechanics",
title = "Viscoelasticity and cell swirling motion",
pages = "425-393",
volume = "55",
doi = "10.1016/bs.aams.2022.05.002"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2022). Viscoelasticity and cell swirling motion. in Advances in Applied Mechanics
Academic Press Inc.., 55, 393-425.
https://doi.org/10.1016/bs.aams.2022.05.002

Pajić-Lijaković I, Milivojević M. Viscoelasticity and cell swirling motion. in Advances in Applied Mechanics. 2022;55:393-425.
doi:10.1016/bs.aams.2022.05.002 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Viscoelasticity and cell swirling motion" in Advances in Applied Mechanics, 55 (2022):393-425,
https://doi.org/10.1016/bs.aams.2022.05.002 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5012
AB  - Long-timescale viscoelasticity caused by collective cell migration (CCM) significantly influences cell rearrangement and induces generation of mechanical waves. The phenomenon represents a product of the active turbulence occurring at low Reynolds number. The generation of mechanical waves has been a subject of intensive research primarily in 2D multicellular systems, while 3D systems have not been considered in this context. The aim of this contribution is to discuss the generation of mechanical waves during 3D CCM in two model systems: (1) the fusion of two-cell aggregates and (2) cell aggregate rounding after uni-axial compression, pointing out that mechanical waves represent a characteristic of CCM in general. Such perturbations are also involved in various biological processes, such as embryogenesis, wound healing and cancer invasion. The inter-relation between the viscoelasticity and the appearance of active turbulence remains poorly understood even in 2D. The phenomenon represents a consequence of the competition between the viscoelastic force and the surface tension force which induces successive stiffening and softening of parts of multicellular systems. The viscoelastic force is a product of the residual cell stress accumulation and its inhomogeneous distribution caused by CCM. This modeling consideration represents a powerful tool to address the generation of mechanical waves in CCM towards an understanding of this important but still controversial topic.
T2  - European Biophysics Journal With Biophysics Letters
T1  - Mechanical waves caused by collective cell migration: generation
EP  - 13
IS  - 1
SP  - 1
VL  - 51
DO  - 10.1007/s00249-021-01581-x
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2022",
abstract = "Long-timescale viscoelasticity caused by collective cell migration (CCM) significantly influences cell rearrangement and induces generation of mechanical waves. The phenomenon represents a product of the active turbulence occurring at low Reynolds number. The generation of mechanical waves has been a subject of intensive research primarily in 2D multicellular systems, while 3D systems have not been considered in this context. The aim of this contribution is to discuss the generation of mechanical waves during 3D CCM in two model systems: (1) the fusion of two-cell aggregates and (2) cell aggregate rounding after uni-axial compression, pointing out that mechanical waves represent a characteristic of CCM in general. Such perturbations are also involved in various biological processes, such as embryogenesis, wound healing and cancer invasion. The inter-relation between the viscoelasticity and the appearance of active turbulence remains poorly understood even in 2D. The phenomenon represents a consequence of the competition between the viscoelastic force and the surface tension force which induces successive stiffening and softening of parts of multicellular systems. The viscoelastic force is a product of the residual cell stress accumulation and its inhomogeneous distribution caused by CCM. This modeling consideration represents a powerful tool to address the generation of mechanical waves in CCM towards an understanding of this important but still controversial topic.",
journal = "European Biophysics Journal With Biophysics Letters",
title = "Mechanical waves caused by collective cell migration: generation",
pages = "13-1",
number = "1",
volume = "51",
doi = "10.1007/s00249-021-01581-x"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2022). Mechanical waves caused by collective cell migration: generation. in European Biophysics Journal With Biophysics Letters, 51(1), 1-13.
https://doi.org/10.1007/s00249-021-01581-x

Pajić-Lijaković I, Milivojević M. Mechanical waves caused by collective cell migration: generation. in European Biophysics Journal With Biophysics Letters. 2022;51(1):1-13.
doi:10.1007/s00249-021-01581-x .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Mechanical waves caused by collective cell migration: generation" in European Biophysics Journal With Biophysics Letters, 51, no. 1 (2022):1-13,
https://doi.org/10.1007/s00249-021-01581-x . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
AU  - Clark, Andrew G.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5185
AB  - Collective cell migration on extracellular matrix (ECM) networks is a key biological process involved in development, tissue homeostasis and diseases such as metastatic cancer. During invasion of epithelial cancers, cell clusters migrate through the surrounding stroma, which is comprised primarily of networks of collagen-I fibers. There is growing evidence that the rheological and topological properties of collagen networks can impact cell behavior and cell migration dynamics. During migration, cells exert mechanical forces on their substrate, resulting in an active remodeling of ECM networks that depends not only on the forces produced, but also on the molecular mechanisms that dictate network rheology. One aspect of collagen network rheology whose role is emerging as a crucial parameter in dictating cell behavior is network viscoelasticity. Dynamic reorganization of ECM networks can induce local changes in network organization and mechanics, which can further feed back on cell migration dynamics and cell-cell rearrangement. A number of studies, including many recent publications, have investigated the mechanisms underlying structural changes to collagen networks in response to mechanical force as well as the role of collagen rheology and topology in regulating cell behavior. In this mini-review, we explore the cause-consequence relationship between collagen network viscoelasticity and cell rearrangements at various spatiotemporal scales. We focus on structural alterations of collagen-I networks during collective cell migration and discuss the main rheological parameters, and in particular the role of viscoelasticity, which can contribute to local matrix stiffening during cell movement and can elicit changes in cell dynamics.
PB  - Frontiers Media S.A.
T2  - Frontiers in Cell and Developmental Biology
T1  - Collective Cell Migration on Collagen-I Networks: The Impact of Matrix Viscoelasticity
EP  - 901026
VL  - 10
DO  - 10.3389/fcell.2022.901026
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan and Clark, Andrew G.",
year = "2022",
abstract = "Collective cell migration on extracellular matrix (ECM) networks is a key biological process involved in development, tissue homeostasis and diseases such as metastatic cancer. During invasion of epithelial cancers, cell clusters migrate through the surrounding stroma, which is comprised primarily of networks of collagen-I fibers. There is growing evidence that the rheological and topological properties of collagen networks can impact cell behavior and cell migration dynamics. During migration, cells exert mechanical forces on their substrate, resulting in an active remodeling of ECM networks that depends not only on the forces produced, but also on the molecular mechanisms that dictate network rheology. One aspect of collagen network rheology whose role is emerging as a crucial parameter in dictating cell behavior is network viscoelasticity. Dynamic reorganization of ECM networks can induce local changes in network organization and mechanics, which can further feed back on cell migration dynamics and cell-cell rearrangement. A number of studies, including many recent publications, have investigated the mechanisms underlying structural changes to collagen networks in response to mechanical force as well as the role of collagen rheology and topology in regulating cell behavior. In this mini-review, we explore the cause-consequence relationship between collagen network viscoelasticity and cell rearrangements at various spatiotemporal scales. We focus on structural alterations of collagen-I networks during collective cell migration and discuss the main rheological parameters, and in particular the role of viscoelasticity, which can contribute to local matrix stiffening during cell movement and can elicit changes in cell dynamics.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Cell and Developmental Biology",
title = "Collective Cell Migration on Collagen-I Networks: The Impact of Matrix Viscoelasticity",
pages = "901026",
volume = "10",
doi = "10.3389/fcell.2022.901026"
}

Pajić-Lijaković, I., Milivojević, M.,& Clark, A. G.. (2022). Collective Cell Migration on Collagen-I Networks: The Impact of Matrix Viscoelasticity. in Frontiers in Cell and Developmental Biology
Frontiers Media S.A.., 10.
https://doi.org/10.3389/fcell.2022.901026

Pajić-Lijaković I, Milivojević M, Clark AG. Collective Cell Migration on Collagen-I Networks: The Impact of Matrix Viscoelasticity. in Frontiers in Cell and Developmental Biology. 2022;10:null-901026.
doi:10.3389/fcell.2022.901026 .

Pajić-Lijaković, Ivana, Milivojević, Milan, Clark, Andrew G., "Collective Cell Migration on Collagen-I Networks: The Impact of Matrix Viscoelasticity" in Frontiers in Cell and Developmental Biology, 10 (2022),
https://doi.org/10.3389/fcell.2022.901026 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5383
AB  - Cell rearrangement caused by collective cell migration (CCM) during free expansion of epithelial monolayers has become a landmark in our current understanding of fundamental biological processes such as tissue development, regeneration, wound healing or cancer invasion. Cell spreading causes formation of mechanical waves which has a feedback effect on cell rearrangement and can lead to the cell jamming state. The mechanical waves describe oscillatory changes in cell velocity, as well as, the rheological parameters that affect them. The velocity oscillations, obtained at a time scale of hours, are in the form of forward and backward flows. Collision of forward and backward flows can induce an increase in the cell compressive stress accompanied with cell packing density which have a feedback impact on cell mobility, tissue viscoelasticity and alters the tissue stiffness. The tissue stiffness depends on the cell packing density and the active/passive (i.e. migrating/resting) state of single cells and can be used as an indicator of cell jamming state transition. Since cell stiffness can be measured it may directly show in which state the multicellular system is. In this work a review of existing modeling approaches is given along with assortment of published experimental findings, in order to invite experimentalists to test given theoretical considerations in multicellular systems.
PB  - Elsevier Ltd.
T2  - Progress in Biophysics and Molecular Biology
T1  - The role of viscoelasticity in long time cell rearrangement
EP  - 71
SP  - 60
VL  - 173
DO  - 10.1016/j.pbiomolbio.2022.05.005
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2022",
abstract = "Cell rearrangement caused by collective cell migration (CCM) during free expansion of epithelial monolayers has become a landmark in our current understanding of fundamental biological processes such as tissue development, regeneration, wound healing or cancer invasion. Cell spreading causes formation of mechanical waves which has a feedback effect on cell rearrangement and can lead to the cell jamming state. The mechanical waves describe oscillatory changes in cell velocity, as well as, the rheological parameters that affect them. The velocity oscillations, obtained at a time scale of hours, are in the form of forward and backward flows. Collision of forward and backward flows can induce an increase in the cell compressive stress accompanied with cell packing density which have a feedback impact on cell mobility, tissue viscoelasticity and alters the tissue stiffness. The tissue stiffness depends on the cell packing density and the active/passive (i.e. migrating/resting) state of single cells and can be used as an indicator of cell jamming state transition. Since cell stiffness can be measured it may directly show in which state the multicellular system is. In this work a review of existing modeling approaches is given along with assortment of published experimental findings, in order to invite experimentalists to test given theoretical considerations in multicellular systems.",
publisher = "Elsevier Ltd.",
journal = "Progress in Biophysics and Molecular Biology",
title = "The role of viscoelasticity in long time cell rearrangement",
pages = "71-60",
volume = "173",
doi = "10.1016/j.pbiomolbio.2022.05.005"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2022). The role of viscoelasticity in long time cell rearrangement. in Progress in Biophysics and Molecular Biology
Elsevier Ltd.., 173, 60-71.
https://doi.org/10.1016/j.pbiomolbio.2022.05.005

Pajić-Lijaković I, Milivojević M. The role of viscoelasticity in long time cell rearrangement. in Progress in Biophysics and Molecular Biology. 2022;173:60-71.
doi:10.1016/j.pbiomolbio.2022.05.005 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "The role of viscoelasticity in long time cell rearrangement" in Progress in Biophysics and Molecular Biology, 173 (2022):60-71,
https://doi.org/10.1016/j.pbiomolbio.2022.05.005 . .

TY  - JOUR
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Milivojević, Milan
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5281
AB  - In this study, a heterofunctional carrier was obtained by modification of a macroporous polystyrene ion exchange resin with primary amino groups (Purolite® A109) with epichlorohydrin, in view of achieving covalent immobilization of fructosyltransferase (FTase) from a complex enzyme mixture, Pectinex® Ultra SP-L, that is responsible for the synthesis of functionally active fructo-oligosaccharides (FOS). A two-step immobilization protocol, comprising the physical adsorption of FTase performed at pH 4 and subsequent buffer exchange to promote the establishment of covalent bonds at pH 9, was proposed for obtaining a highly active immobilized preparation (243 IU g−1 of support). Additionally, this protocol provided development of a preparation with 20 times more prominent expressed activity of FTase compared to the commercial preparation with predominant pectinase activity. The obtained immobilized preparation was further tested in batch and air-lift reactor systems for FOS synthesis, yielding 52.8% and 54.7% of FOS in total carbohydrates, respectively. Finally, the continuous production of FOS in the air-lift reactor was established for 7 days, with an average FOS yield of 52.5%. Accordingly, it is demonstrated that the immobilization process enabled the development of preparations with exceptional potential for industrial implementation.
PB  - Royal Society of Chemistry
T2  - Reaction Chemistry and Engineering
T1  - Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides
EP  - 2526
IS  - 12
SP  - 2518
VL  - 7
DO  - 10.1039/d2re00182a
ER  - 

@article{
author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Ćorović, Marija and Milivojević, Ana and Milivojević, Milan and Bezbradica, Dejan",
year = "2022",
abstract = "In this study, a heterofunctional carrier was obtained by modification of a macroporous polystyrene ion exchange resin with primary amino groups (Purolite® A109) with epichlorohydrin, in view of achieving covalent immobilization of fructosyltransferase (FTase) from a complex enzyme mixture, Pectinex® Ultra SP-L, that is responsible for the synthesis of functionally active fructo-oligosaccharides (FOS). A two-step immobilization protocol, comprising the physical adsorption of FTase performed at pH 4 and subsequent buffer exchange to promote the establishment of covalent bonds at pH 9, was proposed for obtaining a highly active immobilized preparation (243 IU g−1 of support). Additionally, this protocol provided development of a preparation with 20 times more prominent expressed activity of FTase compared to the commercial preparation with predominant pectinase activity. The obtained immobilized preparation was further tested in batch and air-lift reactor systems for FOS synthesis, yielding 52.8% and 54.7% of FOS in total carbohydrates, respectively. Finally, the continuous production of FOS in the air-lift reactor was established for 7 days, with an average FOS yield of 52.5%. Accordingly, it is demonstrated that the immobilization process enabled the development of preparations with exceptional potential for industrial implementation.",
publisher = "Royal Society of Chemistry",
journal = "Reaction Chemistry and Engineering",
title = "Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides",
pages = "2526-2518",
number = "12",
volume = "7",
doi = "10.1039/d2re00182a"
}

Veljković, M., Simović, M., Banjanac, K., Ćorović, M., Milivojević, A., Milivojević, M.,& Bezbradica, D.. (2022). Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides. in Reaction Chemistry and Engineering
Royal Society of Chemistry., 7(12), 2518-2526.
https://doi.org/10.1039/d2re00182a

Veljković M, Simović M, Banjanac K, Ćorović M, Milivojević A, Milivojević M, Bezbradica D. Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides. in Reaction Chemistry and Engineering. 2022;7(12):2518-2526.
doi:10.1039/d2re00182a .

Veljković, Milica, Simović, Milica, Banjanac, Katarina, Ćorović, Marija, Milivojević, Ana, Milivojević, Milan, Bezbradica, Dejan, "Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides" in Reaction Chemistry and Engineering, 7, no. 12 (2022):2518-2526,
https://doi.org/10.1039/d2re00182a . .

TY  - JOUR
AU  - Jonovic, Marko
AU  - Zuza, Milena
AU  - Đorđević, Verica
AU  - Šekuljica, Nataša
AU  - Milivojević, Milan
AU  - Jugovic, Branimir
AU  - Bugarski, Branko
AU  - Knežević-Jugović, Zorica
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4879
AB  - Enzymatic hydrolysis of food proteins is convenient method to improve their functional properties and physiological activity. Herein, the successful covalent attachment of alcalase on alginate micron and submicron beads using the carbodiimide based chemistry reaction and the subsequent application of the beads for egg white and soy proteins hydrolysis were studied. In addition to the electrostatic extrusion technique (EE) previously used by others, the potential utilization of a novel ultrasonic spray atomization technique without drying (UA) and with drying (UAD) for alginate submicron beads production has been attempted. The immobilization parameters were optimized on microbeads obtained by EE technique (803 +/- 23 mu m) with respect to enzyme loading and alcalase activity. UA and UAD techniques resulted in much smaller particles (607 +/- 103 nm and 394 +/- 51 nm in diameter, respectively), enabling even higher enzyme loading of 671.6 +/- 4 mg g(-1) on the carrier and the highest immobilized alcalase activity of 2716.1 IU g(-1) in the standard reaction. The UAD biocatalyst exhibited also better performances in the real food system based on egg white or soy proteins. It has been shown that the immobilized alcalase can be reused in seven successive soy protein hydrolysis cycles with a little decrease in the activity.
T2  - Catalysts
T1  - Immobilized Alcalase on Micron- and Submicron-Sized Alginate Beads as a Potential Biocatalyst for Hydrolysis of Food Proteins
IS  - 3
VL  - 11
DO  - 10.3390/catal11030305
ER  - 

@article{
author = "Jonovic, Marko and Zuza, Milena and Đorđević, Verica and Šekuljica, Nataša and Milivojević, Milan and Jugovic, Branimir and Bugarski, Branko and Knežević-Jugović, Zorica",
year = "2021",
abstract = "Enzymatic hydrolysis of food proteins is convenient method to improve their functional properties and physiological activity. Herein, the successful covalent attachment of alcalase on alginate micron and submicron beads using the carbodiimide based chemistry reaction and the subsequent application of the beads for egg white and soy proteins hydrolysis were studied. In addition to the electrostatic extrusion technique (EE) previously used by others, the potential utilization of a novel ultrasonic spray atomization technique without drying (UA) and with drying (UAD) for alginate submicron beads production has been attempted. The immobilization parameters were optimized on microbeads obtained by EE technique (803 +/- 23 mu m) with respect to enzyme loading and alcalase activity. UA and UAD techniques resulted in much smaller particles (607 +/- 103 nm and 394 +/- 51 nm in diameter, respectively), enabling even higher enzyme loading of 671.6 +/- 4 mg g(-1) on the carrier and the highest immobilized alcalase activity of 2716.1 IU g(-1) in the standard reaction. The UAD biocatalyst exhibited also better performances in the real food system based on egg white or soy proteins. It has been shown that the immobilized alcalase can be reused in seven successive soy protein hydrolysis cycles with a little decrease in the activity.",
journal = "Catalysts",
title = "Immobilized Alcalase on Micron- and Submicron-Sized Alginate Beads as a Potential Biocatalyst for Hydrolysis of Food Proteins",
number = "3",
volume = "11",
doi = "10.3390/catal11030305"
}

Jonovic, M., Zuza, M., Đorđević, V., Šekuljica, N., Milivojević, M., Jugovic, B., Bugarski, B.,& Knežević-Jugović, Z.. (2021). Immobilized Alcalase on Micron- and Submicron-Sized Alginate Beads as a Potential Biocatalyst for Hydrolysis of Food Proteins. in Catalysts, 11(3).
https://doi.org/10.3390/catal11030305

Jonovic M, Zuza M, Đorđević V, Šekuljica N, Milivojević M, Jugovic B, Bugarski B, Knežević-Jugović Z. Immobilized Alcalase on Micron- and Submicron-Sized Alginate Beads as a Potential Biocatalyst for Hydrolysis of Food Proteins. in Catalysts. 2021;11(3).
doi:10.3390/catal11030305 .

Jonovic, Marko, Zuza, Milena, Đorđević, Verica, Šekuljica, Nataša, Milivojević, Milan, Jugovic, Branimir, Bugarski, Branko, Knežević-Jugović, Zorica, "Immobilized Alcalase on Micron- and Submicron-Sized Alginate Beads as a Potential Biocatalyst for Hydrolysis of Food Proteins" in Catalysts, 11, no. 3 (2021),
https://doi.org/10.3390/catal11030305 . .

TY  - JOUR
AU  - Zdujic, Aleksandar
AU  - Trivunac, Katarina
AU  - Pejic, Biljana
AU  - Vukčević, Marija
AU  - Kostić, Mirjana
AU  - Milivojević, Milan
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4969
AB  - Ca-alginate particles (CA) and alginate-impregnated hemp fibers (AH) (both, as prepared, dried, and rehydrated) were used as adsorbents for removal of Ni (II) ions from water. Adsorption was examined in the batch system and experimentally obtained data were analyzed by both linear and nonlinear curve fitting using pseudo-first and pseudo-second-order rate, as well as Langmuir, Freundlich, and Sips equations. The concentration of Ni (II) ions was measured by atomic absorption spectrophotometry, while the Fourier transform infrared spectroscopy was used for characterization of adsorbent surface, before and after the adsorption. Although all tested samples showed similar adsorption capacities of 12 mg/g, it was found that rehydration improves adsorption characteristics of AH and increases the removal efficiency above 90 %. Adsorption of Ni (II) ions can be sufficiently described by both kinetic models and Sips isotherm equation, and this relatively fast process presumably occurs through the mechanism of physisorption and ion-exchange. The obtained results proved that Ca-alginate particles and alginate-impregnated hemp fibers have good potential to reduce nickel concentrations in a cost-effective and efficient manner.
T2  - Fibers and Polymers
T1  - A Comparative Study of Ni (II) Removal from Aqueous Solutions on Ca-Alginate Beads and Alginate-Impregnated Hemp Fibers
EP  - 18
IS  - 1
SP  - 9
VL  - 22
DO  - 10.1007/s12221-021-9814-6
ER  - 

@article{
author = "Zdujic, Aleksandar and Trivunac, Katarina and Pejic, Biljana and Vukčević, Marija and Kostić, Mirjana and Milivojević, Milan",
year = "2021",
abstract = "Ca-alginate particles (CA) and alginate-impregnated hemp fibers (AH) (both, as prepared, dried, and rehydrated) were used as adsorbents for removal of Ni (II) ions from water. Adsorption was examined in the batch system and experimentally obtained data were analyzed by both linear and nonlinear curve fitting using pseudo-first and pseudo-second-order rate, as well as Langmuir, Freundlich, and Sips equations. The concentration of Ni (II) ions was measured by atomic absorption spectrophotometry, while the Fourier transform infrared spectroscopy was used for characterization of adsorbent surface, before and after the adsorption. Although all tested samples showed similar adsorption capacities of 12 mg/g, it was found that rehydration improves adsorption characteristics of AH and increases the removal efficiency above 90 %. Adsorption of Ni (II) ions can be sufficiently described by both kinetic models and Sips isotherm equation, and this relatively fast process presumably occurs through the mechanism of physisorption and ion-exchange. The obtained results proved that Ca-alginate particles and alginate-impregnated hemp fibers have good potential to reduce nickel concentrations in a cost-effective and efficient manner.",
journal = "Fibers and Polymers",
title = "A Comparative Study of Ni (II) Removal from Aqueous Solutions on Ca-Alginate Beads and Alginate-Impregnated Hemp Fibers",
pages = "18-9",
number = "1",
volume = "22",
doi = "10.1007/s12221-021-9814-6"
}

Zdujic, A., Trivunac, K., Pejic, B., Vukčević, M., Kostić, M.,& Milivojević, M.. (2021). A Comparative Study of Ni (II) Removal from Aqueous Solutions on Ca-Alginate Beads and Alginate-Impregnated Hemp Fibers. in Fibers and Polymers, 22(1), 9-18.
https://doi.org/10.1007/s12221-021-9814-6

Zdujic A, Trivunac K, Pejic B, Vukčević M, Kostić M, Milivojević M. A Comparative Study of Ni (II) Removal from Aqueous Solutions on Ca-Alginate Beads and Alginate-Impregnated Hemp Fibers. in Fibers and Polymers. 2021;22(1):9-18.
doi:10.1007/s12221-021-9814-6 .

Zdujic, Aleksandar, Trivunac, Katarina, Pejic, Biljana, Vukčević, Marija, Kostić, Mirjana, Milivojević, Milan, "A Comparative Study of Ni (II) Removal from Aqueous Solutions on Ca-Alginate Beads and Alginate-Impregnated Hemp Fibers" in Fibers and Polymers, 22, no. 1 (2021):9-18,
https://doi.org/10.1007/s12221-021-9814-6 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4868
AB  - Although collective cell migration (CCM) is a highly coordinated migratory mode, perturbations in the form of jamming state transitions and vice versa often occur even in 2D. These perturbations are involved in various biological processes, such as embryogenesis, wound healing, and cancer invasion. CCM induces accumulation of cell residual stress, which has a feedback impact to cell packing density. Density-mediated change of cell mobility influences the state of viscoelasticity of multicellular systems and on that base the jamming state transition. Although a good comprehension of how cells collectively migrate by following molecular rules has been generated, the impact of cellular rearrangements on cell viscoelasticity remains less understood, thus considering that the density-driven evolution of viscoelasticity caused by reduction of cell mobility could result in a powerful tool in order to address the contribution of cell jamming state transition in CCM and help to understand this important but still a controversial topic. In this work a review of existing literature in CCM modeling is given along with an assortment of published experimental findings, in order to invite experimentalists to test the given theoretical considerations in multicellular systems. In addition, five viscoelastic states gained within three regimes: (1) convective regime, (2) conductive regime, and (3) damped-conductive regime, which were discussed with special emphasis of jamming and unjamming states.
T2  - European Physical Journal Plus
T1  - Viscoelasticity and cell jamming state transition
IS  - 7
VL  - 136
DO  - 10.1140/epjp/s13360-021-01730-3
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2021",
abstract = "Although collective cell migration (CCM) is a highly coordinated migratory mode, perturbations in the form of jamming state transitions and vice versa often occur even in 2D. These perturbations are involved in various biological processes, such as embryogenesis, wound healing, and cancer invasion. CCM induces accumulation of cell residual stress, which has a feedback impact to cell packing density. Density-mediated change of cell mobility influences the state of viscoelasticity of multicellular systems and on that base the jamming state transition. Although a good comprehension of how cells collectively migrate by following molecular rules has been generated, the impact of cellular rearrangements on cell viscoelasticity remains less understood, thus considering that the density-driven evolution of viscoelasticity caused by reduction of cell mobility could result in a powerful tool in order to address the contribution of cell jamming state transition in CCM and help to understand this important but still a controversial topic. In this work a review of existing literature in CCM modeling is given along with an assortment of published experimental findings, in order to invite experimentalists to test the given theoretical considerations in multicellular systems. In addition, five viscoelastic states gained within three regimes: (1) convective regime, (2) conductive regime, and (3) damped-conductive regime, which were discussed with special emphasis of jamming and unjamming states.",
journal = "European Physical Journal Plus",
title = "Viscoelasticity and cell jamming state transition",
number = "7",
volume = "136",
doi = "10.1140/epjp/s13360-021-01730-3"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2021). Viscoelasticity and cell jamming state transition. in European Physical Journal Plus, 136(7).
https://doi.org/10.1140/epjp/s13360-021-01730-3

Pajić-Lijaković I, Milivojević M. Viscoelasticity and cell jamming state transition. in European Physical Journal Plus. 2021;136(7).
doi:10.1140/epjp/s13360-021-01730-3 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Viscoelasticity and cell jamming state transition" in European Physical Journal Plus, 136, no. 7 (2021),
https://doi.org/10.1140/epjp/s13360-021-01730-3 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4965
AB  - Collective cell migration (CCM), a highly coordinated and fine-tuned migratory mode, is involved in a plethora of biological processes, such as embryogenesis, tissue repair and cancer invasion. Although a good comprehension of how cells collectively migrate by following molecular rules has been generated, the impact of cellular rearrangements on collective migration remains less understood. Thus, considering CCM from a multi-scale quantitative approach could result in a powerful tool to address the contribution of cellular rearrangements in CCM and help to understand this important but still controversial topic. In this work, a review of existing literature in CCM modeling at different scales is given along with assortment of published experimental findings, to invite experimentalists to test given theoretical considerations in multicellular systems. In addition, three different time and space scales (free or weakly connected cells, cluster of cells and collision fronts of different cells clusters) are considered and the multi-scale nature of those processes was discussed with special emphasis of jamming and unjamming states.
T2  - European Biophysics Journal With Biophysics Letters
T1  - Multiscale nature of cell rearrangement caused by collective cell migration
EP  - 14
IS  - 1
SP  - 1
VL  - 50
DO  - 10.1007/s00249-021-01496-7
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan",
year = "2021",
abstract = "Collective cell migration (CCM), a highly coordinated and fine-tuned migratory mode, is involved in a plethora of biological processes, such as embryogenesis, tissue repair and cancer invasion. Although a good comprehension of how cells collectively migrate by following molecular rules has been generated, the impact of cellular rearrangements on collective migration remains less understood. Thus, considering CCM from a multi-scale quantitative approach could result in a powerful tool to address the contribution of cellular rearrangements in CCM and help to understand this important but still controversial topic. In this work, a review of existing literature in CCM modeling at different scales is given along with assortment of published experimental findings, to invite experimentalists to test given theoretical considerations in multicellular systems. In addition, three different time and space scales (free or weakly connected cells, cluster of cells and collision fronts of different cells clusters) are considered and the multi-scale nature of those processes was discussed with special emphasis of jamming and unjamming states.",
journal = "European Biophysics Journal With Biophysics Letters",
title = "Multiscale nature of cell rearrangement caused by collective cell migration",
pages = "14-1",
number = "1",
volume = "50",
doi = "10.1007/s00249-021-01496-7"
}

Pajić-Lijaković, I.,& Milivojević, M.. (2021). Multiscale nature of cell rearrangement caused by collective cell migration. in European Biophysics Journal With Biophysics Letters, 50(1), 1-14.
https://doi.org/10.1007/s00249-021-01496-7

Pajić-Lijaković I, Milivojević M. Multiscale nature of cell rearrangement caused by collective cell migration. in European Biophysics Journal With Biophysics Letters. 2021;50(1):1-14.
doi:10.1007/s00249-021-01496-7 .

Pajić-Lijaković, Ivana, Milivojević, Milan, "Multiscale nature of cell rearrangement caused by collective cell migration" in European Biophysics Journal With Biophysics Letters, 50, no. 1 (2021):1-14,
https://doi.org/10.1007/s00249-021-01496-7 . .