TY  - JOUR
AU  - Bogdanović, Mihajlo
AU  - Mladenović, Dragana
AU  - Mojović, Ljiljana
AU  - Đuriš, Jelena
AU  - Đukić-Vuković, Aleksandra
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7346
AB  - The last decade provided significant advances in the understanding of microbiota and its role in human health. Probiotics are live microorganisms with proven benefits for the host and were mostly studied in the context of gut health, but they can also confer significant benefits for oral health, mainly in the treatment of gingivitis. Postbiotics are cell-free extracts and metabolites of microorganisms which can provide additional preventive and therapeutic value for human health. This opens opportunities for new preventive or therapeutic formulations for oral administration. The microorganisms that colonize the oral cavity, their role in oral health and disease, as well as the probiotics and postbiotics which could have beneficial effects in this complex environment were discussed. The aim of this study was to review, analyse and discuss novel probiotic and postbiotic formulations intended for oral administration that could be of great preventive and therapeutic importance. A special attention has been put on the formulation of the pharmaceutical dosage forms that are expected to provide new benefits for the patients and technological advantages relevant for industry. An adequate dosage form could significantly enhance the efficiency of these products.
PB  - Faculty of Pharmaceutical Sciences of the University of São Paulo
T2  - Brazilian Journal of Pharmaceutical Sciences
T1  - Intraoral administration of probiotics and postbiotics: An overview of microorganisms and formulation strategies
SP  - e23272
VL  - 60
DO  - 10.1590/s2175-97902024e23272
ER  - 

@article{
author = "Bogdanović, Mihajlo and Mladenović, Dragana and Mojović, Ljiljana and Đuriš, Jelena and Đukić-Vuković, Aleksandra",
year = "2024",
abstract = "The last decade provided significant advances in the understanding of microbiota and its role in human health. Probiotics are live microorganisms with proven benefits for the host and were mostly studied in the context of gut health, but they can also confer significant benefits for oral health, mainly in the treatment of gingivitis. Postbiotics are cell-free extracts and metabolites of microorganisms which can provide additional preventive and therapeutic value for human health. This opens opportunities for new preventive or therapeutic formulations for oral administration. The microorganisms that colonize the oral cavity, their role in oral health and disease, as well as the probiotics and postbiotics which could have beneficial effects in this complex environment were discussed. The aim of this study was to review, analyse and discuss novel probiotic and postbiotic formulations intended for oral administration that could be of great preventive and therapeutic importance. A special attention has been put on the formulation of the pharmaceutical dosage forms that are expected to provide new benefits for the patients and technological advantages relevant for industry. An adequate dosage form could significantly enhance the efficiency of these products.",
publisher = "Faculty of Pharmaceutical Sciences of the University of São Paulo",
journal = "Brazilian Journal of Pharmaceutical Sciences",
title = "Intraoral administration of probiotics and postbiotics: An overview of microorganisms and formulation strategies",
pages = "e23272",
volume = "60",
doi = "10.1590/s2175-97902024e23272"
}

Bogdanović, M., Mladenović, D., Mojović, L., Đuriš, J.,& Đukić-Vuković, A.. (2024). Intraoral administration of probiotics and postbiotics: An overview of microorganisms and formulation strategies. in Brazilian Journal of Pharmaceutical Sciences
Faculty of Pharmaceutical Sciences of the University of São Paulo., 60, e23272.
https://doi.org/10.1590/s2175-97902024e23272

Bogdanović M, Mladenović D, Mojović L, Đuriš J, Đukić-Vuković A. Intraoral administration of probiotics and postbiotics: An overview of microorganisms and formulation strategies. in Brazilian Journal of Pharmaceutical Sciences. 2024;60:e23272.
doi:10.1590/s2175-97902024e23272 .

Bogdanović, Mihajlo, Mladenović, Dragana, Mojović, Ljiljana, Đuriš, Jelena, Đukić-Vuković, Aleksandra, "Intraoral administration of probiotics and postbiotics: An overview of microorganisms and formulation strategies" in Brazilian Journal of Pharmaceutical Sciences, 60 (2024):e23272,
https://doi.org/10.1590/s2175-97902024e23272 . .

TY  - CONF
AU  - Mladenović, Dragana
AU  - Grbić, Jovana
AU  - Petrović, Predrag
AU  - Đukić-Vuković, Aleksandra
AU  - Lazović, Saša
AU  - Mojović, Ljiljana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6738
AB  - Lactic acid bacteria have an important role in food production, as probiotics and producers of lactic acid. Due to the generation of oxygen and nitrogen-based reactive species and UV radiation, non-thermal plasma (NTP) was found to be effective in microbial inactivation and food processing. In this study, we investigated the effect of atmospheric NTP on Lacticaseibacillus paracasei NRRL B-4564 survival, membrane alternations, and the response of treated cells to acidic stress. 
Cell suspensions in water were subjected to different treatment time intervals using a custom-made plasma needle. Argon was used as a feed gas, with a flow of 0.5 slm, while the distance between the needle tip and suspension surface was 1.5 cm. Immediately after the treatment, the viable cell number was estimated by the pour plate method, while cell membrane alternations were studied by analyzing zeta potential and membrane permeability (Crystal Violet assay). To ascertain if sub-lethal NTP stress could influence L. paracasei survival in an acidic environment, NTP-treated cells were challenged by pH 2.5 for 3h.
The results demonstrate that the negative surface potential of the bacterial membrane (-29.83±1.49 mV for untreated) was gradually shifted towards neutrality (-7.59±0.54 mV after 180 s) with prolonged treatment time. Increasing cell exposure to NTP resulted in higher membrane permeability, which was correlated with viable cell reduction. The cells exposed to shorter treatment time (30 and 60 s) kept viability and showed better survival in low pH compared to untreated cells, suggesting the application of NTP in probiotic food processing.
PB  - Federation of European Microbiological Societies (FEMS)
C3  - Abstract Book / 10th Congress of European Microbiologists, FEMS2023, 9-13 July 2023, Hamburg, Germany
T1  - Sub-lethal exposure of Lacticaseibacillus paracasei to atmospheric nonthermal plasma alters its membrane and response to low pH
SP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6738
ER  - 

@conference{
author = "Mladenović, Dragana and Grbić, Jovana and Petrović, Predrag and Đukić-Vuković, Aleksandra and Lazović, Saša and Mojović, Ljiljana",
year = "2023",
abstract = "Lactic acid bacteria have an important role in food production, as probiotics and producers of lactic acid. Due to the generation of oxygen and nitrogen-based reactive species and UV radiation, non-thermal plasma (NTP) was found to be effective in microbial inactivation and food processing. In this study, we investigated the effect of atmospheric NTP on Lacticaseibacillus paracasei NRRL B-4564 survival, membrane alternations, and the response of treated cells to acidic stress. 
Cell suspensions in water were subjected to different treatment time intervals using a custom-made plasma needle. Argon was used as a feed gas, with a flow of 0.5 slm, while the distance between the needle tip and suspension surface was 1.5 cm. Immediately after the treatment, the viable cell number was estimated by the pour plate method, while cell membrane alternations were studied by analyzing zeta potential and membrane permeability (Crystal Violet assay). To ascertain if sub-lethal NTP stress could influence L. paracasei survival in an acidic environment, NTP-treated cells were challenged by pH 2.5 for 3h.
The results demonstrate that the negative surface potential of the bacterial membrane (-29.83±1.49 mV for untreated) was gradually shifted towards neutrality (-7.59±0.54 mV after 180 s) with prolonged treatment time. Increasing cell exposure to NTP resulted in higher membrane permeability, which was correlated with viable cell reduction. The cells exposed to shorter treatment time (30 and 60 s) kept viability and showed better survival in low pH compared to untreated cells, suggesting the application of NTP in probiotic food processing.",
publisher = "Federation of European Microbiological Societies (FEMS)",
journal = "Abstract Book / 10th Congress of European Microbiologists, FEMS2023, 9-13 July 2023, Hamburg, Germany",
title = "Sub-lethal exposure of Lacticaseibacillus paracasei to atmospheric nonthermal plasma alters its membrane and response to low pH",
pages = "40",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6738"
}

Mladenović, D., Grbić, J., Petrović, P., Đukić-Vuković, A., Lazović, S.,& Mojović, L.. (2023). Sub-lethal exposure of Lacticaseibacillus paracasei to atmospheric nonthermal plasma alters its membrane and response to low pH. in Abstract Book / 10th Congress of European Microbiologists, FEMS2023, 9-13 July 2023, Hamburg, Germany
Federation of European Microbiological Societies (FEMS)., 40.
https://hdl.handle.net/21.15107/rcub_technorep_6738

Mladenović D, Grbić J, Petrović P, Đukić-Vuković A, Lazović S, Mojović L. Sub-lethal exposure of Lacticaseibacillus paracasei to atmospheric nonthermal plasma alters its membrane and response to low pH. in Abstract Book / 10th Congress of European Microbiologists, FEMS2023, 9-13 July 2023, Hamburg, Germany. 2023;:40.
https://hdl.handle.net/21.15107/rcub_technorep_6738 .

Mladenović, Dragana, Grbić, Jovana, Petrović, Predrag, Đukić-Vuković, Aleksandra, Lazović, Saša, Mojović, Ljiljana, "Sub-lethal exposure of Lacticaseibacillus paracasei to atmospheric nonthermal plasma alters its membrane and response to low pH" in Abstract Book / 10th Congress of European Microbiologists, FEMS2023, 9-13 July 2023, Hamburg, Germany (2023):40,
https://hdl.handle.net/21.15107/rcub_technorep_6738 .

TY  - JOUR
AU  - Grbić, Jovana
AU  - Bogdanović, Mihajlo
AU  - Mladenović, Dragana
AU  - Lazović, Saša
AU  - Mojović, Ljiljana
AU  - Đukić-Vuković, Aleksandra
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7139
AB  - Traditional extraction methods involve the use of polar solvents and/or high temperatures. Both are often energy or time-consuming and require additional purification steps. This compromises the quality of the extracts obtained. Non-thermal plasma pre-treatment for extraction is a promising technique that could increase the sustainability of the process while following the principles of hurdle technology. In this work, we investigated the effects of plasma treatment on the aqueous extraction of antioxidant compounds from herbal tea by-products. Stinging nettle, hoary willowherb, and St. John’s Wort extracts were treated with plasma at different time intervals. The extracts obtained were analyzed for their antioxidant capacity and total phenolic content. Their potential use as growth media for Ligilactobacillus sp. was also investigated. Hoary willowherb extracts showed the highest total phenolic content and antiox-idant activity. While plasma treatment slightly decreased the antioxidant capacity of these extracts, it improved microbial growth. In contrast to hoary willowherb, the antioxidant capacity of stinging nettle extracts’ was not affected by plasma treatment. These extracts were also better for bacterial growth. When added to 25% v/v MRS broth, stinging nettle extracts achieved a growth rate similar to that of pure MRS broth (109 CFU/ml), demonstrating their potential for microbial biomass production and food and feed supplemen-tation.
AB  - Antioksidansi imaju važnu ulogu u sprečavanju oksidacije masnih kiselina prisutnih u prehrambenim proizvodima, čime značajno produžavaju njihov rok trajanja. Zbog nepovoljnog uticaja pojedinih sintetskih antioksidanasa na zdravlje ljudi, sve je češća upotreba antioksidanasa dobijenih iz prirodnih izvora. Antioksidansi se iz biljnih izvora izoluju ekstrakcijom. Tradicionalne metode ekstrakcije podrazumevaju upotrebu polarnih rastvarača, uz primenu visokih temperatura. Ovi procesi su često energetski nepovoljni, dugo traju i zahtevaju dodatne korake prečišćavanja, kako bi se uklonili toksični rastvarači. Izolovanje biološki aktivnih jedinjenja primenom gasne netermalne plazme kao pretretmana bi moglo da poveća održivost ovog procesa. Osim toga, kombinacijom zelenih rastvarača, poput vode, sa tretmanom plazmom, dobijanje prirodnih antioksidanasa bi bilo u skladu sa tzv. „hardl“ tehnologijom i principima cirkularne ekonomije, kojima se teži u prehrambenoj industriji. Predmet ovog rada je ispitivanje mogućnosti dobijanja prirodnih anti-oksidanasa kombinovanom ekstrakcijom nusproizvoda proizvodnje biljnih čajeva. Vodeni ekstrakti koprive, svilovine i kantariona tre-tirani su plazmom u različitim vremenskim intervalima. Analizirana je antioksidativna aktivnost dobijenih ekstrakata, kao i ukupni sadržaj fenola. Takođe, ispitivana je i mogućnost upotrebe ovih ekstrakata kao fermentacionog medijuma. Najveća antioksidativna aktivnost i najviši sadržaj ukupnih fenola ostvareni su u ekstraktima svilovine. Ove vrednosti su blago opadale sa povećanjem dužine tretmana plazmom, dok je rast bakterija bio poboljšan. Ekstrakti koprive su posedovali značajno manju antioksidativnu aktivnost i niži sadržaj ukupnih fenola od ekstrakata svilovine. Međutim, ovi ekstrakti su bili pogodniji za gajenje bakterija. Dodavanjem 25% MRS bujona u ekstrakte koprive, postignut je približno isti rast Ligilactobacillus salivarius kao u čistom MRS bujonu.
PB  - National Society of Processing and Energy in Agriculture
T2  - Journal on Processing and Energy in Agriculture
T1  - Non-thermal plasma as a pretreatment for extraction of herbal tea by-products
T1  - Ekstrakcija sporednih proizvoda prerade biljnih čajeva uz pretretman gasnom plazmom
EP  - 52
IS  - 1
SP  - 47
VL  - 27
DO  - 10.5937/jpea26-44994
ER  - 

@article{
author = "Grbić, Jovana and Bogdanović, Mihajlo and Mladenović, Dragana and Lazović, Saša and Mojović, Ljiljana and Đukić-Vuković, Aleksandra",
year = "2023",
abstract = "Traditional extraction methods involve the use of polar solvents and/or high temperatures. Both are often energy or time-consuming and require additional purification steps. This compromises the quality of the extracts obtained. Non-thermal plasma pre-treatment for extraction is a promising technique that could increase the sustainability of the process while following the principles of hurdle technology. In this work, we investigated the effects of plasma treatment on the aqueous extraction of antioxidant compounds from herbal tea by-products. Stinging nettle, hoary willowherb, and St. John’s Wort extracts were treated with plasma at different time intervals. The extracts obtained were analyzed for their antioxidant capacity and total phenolic content. Their potential use as growth media for Ligilactobacillus sp. was also investigated. Hoary willowherb extracts showed the highest total phenolic content and antiox-idant activity. While plasma treatment slightly decreased the antioxidant capacity of these extracts, it improved microbial growth. In contrast to hoary willowherb, the antioxidant capacity of stinging nettle extracts’ was not affected by plasma treatment. These extracts were also better for bacterial growth. When added to 25% v/v MRS broth, stinging nettle extracts achieved a growth rate similar to that of pure MRS broth (109 CFU/ml), demonstrating their potential for microbial biomass production and food and feed supplemen-tation., Antioksidansi imaju važnu ulogu u sprečavanju oksidacije masnih kiselina prisutnih u prehrambenim proizvodima, čime značajno produžavaju njihov rok trajanja. Zbog nepovoljnog uticaja pojedinih sintetskih antioksidanasa na zdravlje ljudi, sve je češća upotreba antioksidanasa dobijenih iz prirodnih izvora. Antioksidansi se iz biljnih izvora izoluju ekstrakcijom. Tradicionalne metode ekstrakcije podrazumevaju upotrebu polarnih rastvarača, uz primenu visokih temperatura. Ovi procesi su često energetski nepovoljni, dugo traju i zahtevaju dodatne korake prečišćavanja, kako bi se uklonili toksični rastvarači. Izolovanje biološki aktivnih jedinjenja primenom gasne netermalne plazme kao pretretmana bi moglo da poveća održivost ovog procesa. Osim toga, kombinacijom zelenih rastvarača, poput vode, sa tretmanom plazmom, dobijanje prirodnih antioksidanasa bi bilo u skladu sa tzv. „hardl“ tehnologijom i principima cirkularne ekonomije, kojima se teži u prehrambenoj industriji. Predmet ovog rada je ispitivanje mogućnosti dobijanja prirodnih anti-oksidanasa kombinovanom ekstrakcijom nusproizvoda proizvodnje biljnih čajeva. Vodeni ekstrakti koprive, svilovine i kantariona tre-tirani su plazmom u različitim vremenskim intervalima. Analizirana je antioksidativna aktivnost dobijenih ekstrakata, kao i ukupni sadržaj fenola. Takođe, ispitivana je i mogućnost upotrebe ovih ekstrakata kao fermentacionog medijuma. Najveća antioksidativna aktivnost i najviši sadržaj ukupnih fenola ostvareni su u ekstraktima svilovine. Ove vrednosti su blago opadale sa povećanjem dužine tretmana plazmom, dok je rast bakterija bio poboljšan. Ekstrakti koprive su posedovali značajno manju antioksidativnu aktivnost i niži sadržaj ukupnih fenola od ekstrakata svilovine. Međutim, ovi ekstrakti su bili pogodniji za gajenje bakterija. Dodavanjem 25% MRS bujona u ekstrakte koprive, postignut je približno isti rast Ligilactobacillus salivarius kao u čistom MRS bujonu.",
publisher = "National Society of Processing and Energy in Agriculture",
journal = "Journal on Processing and Energy in Agriculture",
title = "Non-thermal plasma as a pretreatment for extraction of herbal tea by-products, Ekstrakcija sporednih proizvoda prerade biljnih čajeva uz pretretman gasnom plazmom",
pages = "52-47",
number = "1",
volume = "27",
doi = "10.5937/jpea26-44994"
}

Grbić, J., Bogdanović, M., Mladenović, D., Lazović, S., Mojović, L.,& Đukić-Vuković, A.. (2023). Non-thermal plasma as a pretreatment for extraction of herbal tea by-products. in Journal on Processing and Energy in Agriculture
National Society of Processing and Energy in Agriculture., 27(1), 47-52.
https://doi.org/10.5937/jpea26-44994

Grbić J, Bogdanović M, Mladenović D, Lazović S, Mojović L, Đukić-Vuković A. Non-thermal plasma as a pretreatment for extraction of herbal tea by-products. in Journal on Processing and Energy in Agriculture. 2023;27(1):47-52.
doi:10.5937/jpea26-44994 .

Grbić, Jovana, Bogdanović, Mihajlo, Mladenović, Dragana, Lazović, Saša, Mojović, Ljiljana, Đukić-Vuković, Aleksandra, "Non-thermal plasma as a pretreatment for extraction of herbal tea by-products" in Journal on Processing and Energy in Agriculture, 27, no. 1 (2023):47-52,
https://doi.org/10.5937/jpea26-44994 . .

TY  - CONF
AU  - Đukić-Vuković, Aleksandra
AU  - Grbić, Jovana
AU  - Bogdanović, Mihajlo
AU  - Mijin, Dušan
AU  - Lazović, Saša
AU  - Mladenović, Dragana
AU  - Mojović, Ljiljana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6749
AB  - Biomass captures CO2 from the atmosphere when growing. In the same time, approximately 1,4 Gt of available renewable biomass is annually wasted while cereal straw wastes contribute to that with 66%. Huge portion of residues is still burnt, while it is the source of bioactives, fermentable sugars and only natural aromatic polymer-lignin.
The main obstacle for valorization of biomass is the recalcitrant nature of dominantly present lignocellulose and variability in biomass composition which decreases already modest efficiency of acid/alkaline thermal treatment conventionally used in biorefineries. Conventional treatments have low selectivity, generate inhibitory compounds for enzymes or microorganisms used in biorefineries and have high environmental footprint.
Cold plasma treatment (CPT) can induce modifications of cellulosic and hemicellulosic fraction as well as oxidation and depolymerisation of lignin, but CPT lacks selectivity in complex substrates such as agri-food wastes. We combined CPT with Fenton reagent or hydrogen peroxide for treatment of corn stalks as significant lignocellulose agri-industrial waste in Serbia. Chemical properties of treated samples were analysed by FTIR, while carbohydrate fraction was subjected to enzymatic hydrolysis followed by spectrophotometric analysis. We showed that delignification with CPT can be efficiently combined with other oxidative treatments, including hydrogen peroxide and Fenton reagent, while preserving or even improving the enzymatic hydrolysis of carbohydrate fractions. Different chemical modifications were obtained depending on Fe/hydrogen peroxide ratio or hydrogen peroxide concentration, however, low energy CPT combined with other oxidative treatments significantly improves delignification and carbohydrate accessibility while decreasing overall processing time and energy consumption.
Further studies have to evaluate oxidative treatments and CPT sources with different parameters for recovery of all fractions present in lignocellulose. This is essential for sustainable biorefineries on lignocellulose. CPT here shows some additional benefits related to its sterilization and microbial decontamination effect. CPT is already used for surface “etching” and sterilization of grafts in medicine and slowly enters food industry in the similar application field. It has a prospect to improve biomass decomposition under carefully selected conditions as shown here, but it can also help in control of undesired microbiota in open fermentation processes. This could be very valuable for the number of biorefinery processes and contribute significantly to the bioeconomy.
AB  - Biljna biomasa vezuje CO2 iz atmosphere kada raste. U isto vreme, otprilike 1,4 Gt dostupne obnovljive biomase se
godišnje baca, dok slama žitarica koja se smatra otpadom učestvuje sa oko 66% u toj količini. Veliki deo agroindustrijskog
otpada se i dalje spaljuje, dok je istovremeno izvor bioaktivnih jedinjenja, fermentativnih šećera i jedinog prirodnog
aromatičnog polimera –lignina.
Osnovno ograničenje u valorizaciji biomase predstavlja rezistentnost lignoceluloze, dominantne frakcije u većini
sporednih sirovina agroindustrijskog porekla, kao i varijabilnost sastava. Time se dodatno smanjuje i onako ogranična
efikasnost kiselinskih/baznih termalnih tretmana koji se konvencionalno koriste u biorafinerijskim postupcima.
Konvencionalni tretmani imaju malu selektivnost, u njima nastaju inhibitorni proizvodi za enzime i mikroorganizme koje
se koriste u biorafinerijskim postupcima i imaju značajan uticaj na životnu sredinu.
Tretman hladnom plazmom (THP) može izazvati modifikacije na celuloznoj i hemiceluloznoj frakciji, kao i oksidaciju i
depolimerizaciju lignin, ali THP nedostaje selektivnost u kompleksnim supstratima kao što je agro-industrijski otpad. Mi
smo kombinovali THP sa Fentonovim reagensom ili vodonik peroksidom u tretmanu kukuruznih stabljika kao značajnog
izvora lignoceluloze agroindustrijskog porekla u Srbiji. Hemijska svojstva tretiranih uzoraka su analizirana FTIR-om,
dok je ugljenohidratna frakcija bila podvrgnuta enzimskoj hidrolizi i analizirana spektrofotometrijskim metodama.
Pokazali smo da delignifikacija THP može da se efikasno kombinuje sa vodonik peroksidom i Fentonovim reagensom
tako da se očuva ugljenohidratna frakcija pogodna za enzimsku hidrolizu. Dobijene su razlike u hemijskim
modifikacijama u zavisnosti od primenjenog odnosa Fe/vodonik peroksid ili koncentracije vodonik peroksida. Utvrđeno
je da THP može biti kombinovan sa drugim naprednim oksidativnim procesima i značajno unaprediti delignifikaciju i
dostupnost ugljenih hidrata, uz smanjenje ukupnog vremena tretmana i potrošnje energije.
Buduće studije će imati za cilj da utvrde uticaj parametara tremana hladnom plazmom na iskorišćenje svih frakcija
lignoceluloze. Ovo je esencijalno za održivost biorafinerijskih postupaka zasnovanih na lignoceluloznoj biomasi. THP
pokazuje neke dodatne benefite povezane sa sterilizacijom i inaktivacijom mikroorganizama što je značajan efekat za
biotehnološke procese. THP je već korišćen za sterilizaciju površina graftova u medicini i pronalazi svoje mesto i u
prehrambenoj industriji u sličnim oblastima primene. THP ima potencijal da unapredi razgradnju biomase pod pažljivo
odabranim uslovima kao što je pokazano u ovom radu, ali takođe može obezbediti kontrolu neželjenih mikroorganizama
u otvorenim fermentacijama. Ovo može biti veoma značajno za brojne biorafinerijske postupke i unapređenje
bioekonomije generalno.
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
PB  - Novi Sad : University of Novi Sad, Faculty of Agriculture
C3  - Book Of Abstracts / VIII International Conference Sustainable Postharvest And Food Technologies - INOPTEP2023 and XXXV Scientific - Professional Conference Processing And Energy In Agriculture - PTEP2023, Subotica, Palić, 23 – 28. April 2023
T1  - Cold plasma assisted resource recovery from corn stalks as agri-food industry wastes
T1  - Hladna plazma u tretmanu kukuruznih stabljika – mogućnosti za efikasnije iskorišćenje resursa
EP  - 34
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6749
ER  - 

@conference{
author = "Đukić-Vuković, Aleksandra and Grbić, Jovana and Bogdanović, Mihajlo and Mijin, Dušan and Lazović, Saša and Mladenović, Dragana and Mojović, Ljiljana",
year = "2023",
abstract = "Biomass captures CO2 from the atmosphere when growing. In the same time, approximately 1,4 Gt of available renewable biomass is annually wasted while cereal straw wastes contribute to that with 66%. Huge portion of residues is still burnt, while it is the source of bioactives, fermentable sugars and only natural aromatic polymer-lignin.
The main obstacle for valorization of biomass is the recalcitrant nature of dominantly present lignocellulose and variability in biomass composition which decreases already modest efficiency of acid/alkaline thermal treatment conventionally used in biorefineries. Conventional treatments have low selectivity, generate inhibitory compounds for enzymes or microorganisms used in biorefineries and have high environmental footprint.
Cold plasma treatment (CPT) can induce modifications of cellulosic and hemicellulosic fraction as well as oxidation and depolymerisation of lignin, but CPT lacks selectivity in complex substrates such as agri-food wastes. We combined CPT with Fenton reagent or hydrogen peroxide for treatment of corn stalks as significant lignocellulose agri-industrial waste in Serbia. Chemical properties of treated samples were analysed by FTIR, while carbohydrate fraction was subjected to enzymatic hydrolysis followed by spectrophotometric analysis. We showed that delignification with CPT can be efficiently combined with other oxidative treatments, including hydrogen peroxide and Fenton reagent, while preserving or even improving the enzymatic hydrolysis of carbohydrate fractions. Different chemical modifications were obtained depending on Fe/hydrogen peroxide ratio or hydrogen peroxide concentration, however, low energy CPT combined with other oxidative treatments significantly improves delignification and carbohydrate accessibility while decreasing overall processing time and energy consumption.
Further studies have to evaluate oxidative treatments and CPT sources with different parameters for recovery of all fractions present in lignocellulose. This is essential for sustainable biorefineries on lignocellulose. CPT here shows some additional benefits related to its sterilization and microbial decontamination effect. CPT is already used for surface “etching” and sterilization of grafts in medicine and slowly enters food industry in the similar application field. It has a prospect to improve biomass decomposition under carefully selected conditions as shown here, but it can also help in control of undesired microbiota in open fermentation processes. This could be very valuable for the number of biorefinery processes and contribute significantly to the bioeconomy., Biljna biomasa vezuje CO2 iz atmosphere kada raste. U isto vreme, otprilike 1,4 Gt dostupne obnovljive biomase se
godišnje baca, dok slama žitarica koja se smatra otpadom učestvuje sa oko 66% u toj količini. Veliki deo agroindustrijskog
otpada se i dalje spaljuje, dok je istovremeno izvor bioaktivnih jedinjenja, fermentativnih šećera i jedinog prirodnog
aromatičnog polimera –lignina.
Osnovno ograničenje u valorizaciji biomase predstavlja rezistentnost lignoceluloze, dominantne frakcije u većini
sporednih sirovina agroindustrijskog porekla, kao i varijabilnost sastava. Time se dodatno smanjuje i onako ogranična
efikasnost kiselinskih/baznih termalnih tretmana koji se konvencionalno koriste u biorafinerijskim postupcima.
Konvencionalni tretmani imaju malu selektivnost, u njima nastaju inhibitorni proizvodi za enzime i mikroorganizme koje
se koriste u biorafinerijskim postupcima i imaju značajan uticaj na životnu sredinu.
Tretman hladnom plazmom (THP) može izazvati modifikacije na celuloznoj i hemiceluloznoj frakciji, kao i oksidaciju i
depolimerizaciju lignin, ali THP nedostaje selektivnost u kompleksnim supstratima kao što je agro-industrijski otpad. Mi
smo kombinovali THP sa Fentonovim reagensom ili vodonik peroksidom u tretmanu kukuruznih stabljika kao značajnog
izvora lignoceluloze agroindustrijskog porekla u Srbiji. Hemijska svojstva tretiranih uzoraka su analizirana FTIR-om,
dok je ugljenohidratna frakcija bila podvrgnuta enzimskoj hidrolizi i analizirana spektrofotometrijskim metodama.
Pokazali smo da delignifikacija THP može da se efikasno kombinuje sa vodonik peroksidom i Fentonovim reagensom
tako da se očuva ugljenohidratna frakcija pogodna za enzimsku hidrolizu. Dobijene su razlike u hemijskim
modifikacijama u zavisnosti od primenjenog odnosa Fe/vodonik peroksid ili koncentracije vodonik peroksida. Utvrđeno
je da THP može biti kombinovan sa drugim naprednim oksidativnim procesima i značajno unaprediti delignifikaciju i
dostupnost ugljenih hidrata, uz smanjenje ukupnog vremena tretmana i potrošnje energije.
Buduće studije će imati za cilj da utvrde uticaj parametara tremana hladnom plazmom na iskorišćenje svih frakcija
lignoceluloze. Ovo je esencijalno za održivost biorafinerijskih postupaka zasnovanih na lignoceluloznoj biomasi. THP
pokazuje neke dodatne benefite povezane sa sterilizacijom i inaktivacijom mikroorganizama što je značajan efekat za
biotehnološke procese. THP je već korišćen za sterilizaciju površina graftova u medicini i pronalazi svoje mesto i u
prehrambenoj industriji u sličnim oblastima primene. THP ima potencijal da unapredi razgradnju biomase pod pažljivo
odabranim uslovima kao što je pokazano u ovom radu, ali takođe može obezbediti kontrolu neželjenih mikroorganizama
u otvorenim fermentacijama. Ovo može biti veoma značajno za brojne biorafinerijske postupke i unapređenje
bioekonomije generalno.",
publisher = "Novi Sad : National Society of Processing and Energy in Agriculture, Novi Sad : University of Novi Sad, Faculty of Agriculture",
journal = "Book Of Abstracts / VIII International Conference Sustainable Postharvest And Food Technologies - INOPTEP2023 and XXXV Scientific - Professional Conference Processing And Energy In Agriculture - PTEP2023, Subotica, Palić, 23 – 28. April 2023",
title = "Cold plasma assisted resource recovery from corn stalks as agri-food industry wastes, Hladna plazma u tretmanu kukuruznih stabljika – mogućnosti za efikasnije iskorišćenje resursa",
pages = "34-33",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6749"
}

Đukić-Vuković, A., Grbić, J., Bogdanović, M., Mijin, D., Lazović, S., Mladenović, D.,& Mojović, L.. (2023). Cold plasma assisted resource recovery from corn stalks as agri-food industry wastes. in Book Of Abstracts / VIII International Conference Sustainable Postharvest And Food Technologies - INOPTEP2023 and XXXV Scientific - Professional Conference Processing And Energy In Agriculture - PTEP2023, Subotica, Palić, 23 – 28. April 2023
Novi Sad : National Society of Processing and Energy in Agriculture., 33-34.
https://hdl.handle.net/21.15107/rcub_technorep_6749

Đukić-Vuković A, Grbić J, Bogdanović M, Mijin D, Lazović S, Mladenović D, Mojović L. Cold plasma assisted resource recovery from corn stalks as agri-food industry wastes. in Book Of Abstracts / VIII International Conference Sustainable Postharvest And Food Technologies - INOPTEP2023 and XXXV Scientific - Professional Conference Processing And Energy In Agriculture - PTEP2023, Subotica, Palić, 23 – 28. April 2023. 2023;:33-34.
https://hdl.handle.net/21.15107/rcub_technorep_6749 .

Đukić-Vuković, Aleksandra, Grbić, Jovana, Bogdanović, Mihajlo, Mijin, Dušan, Lazović, Saša, Mladenović, Dragana, Mojović, Ljiljana, "Cold plasma assisted resource recovery from corn stalks as agri-food industry wastes" in Book Of Abstracts / VIII International Conference Sustainable Postharvest And Food Technologies - INOPTEP2023 and XXXV Scientific - Professional Conference Processing And Energy In Agriculture - PTEP2023, Subotica, Palić, 23 – 28. April 2023 (2023):33-34,
https://hdl.handle.net/21.15107/rcub_technorep_6749 .

Mojović, L., Mladenović, D., Đukić-Vuković, A., Pejin, J., Kocić-Tanackov, S.,& Despotović, S.. (2023). Tehnološki postupak za poboljšanje kvaliteta pivskog tropa kao hraniva za preživare. .
https://hdl.handle.net/21.15107/rcub_technorep_6748

Mojović L, Mladenović D, Đukić-Vuković A, Pejin J, Kocić-Tanackov S, Despotović S. Tehnološki postupak za poboljšanje kvaliteta pivskog tropa kao hraniva za preživare. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6748 .

Mojović, Ljiljana, Mladenović, Dragana, Đukić-Vuković, Aleksandra, Pejin, Jelena, Kocić-Tanackov, Sunčica, Despotović, Saša, "Tehnološki postupak za poboljšanje kvaliteta pivskog tropa kao hraniva za preživare" (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6748 .

TY  - CONF
AU  - Mladenović, Dragana
AU  - Grbić, Jovana
AU  - Đukić-Vuković, Aleksandra
AU  - Mojović, Ljiljana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6747
PB  - National Technical University of Athens
C3  - 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece
T1  - Fractionation of corncob biomass towards sustainable valorization in biorefinery processes
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6747
ER  - 

@conference{
author = "Mladenović, Dragana and Grbić, Jovana and Đukić-Vuković, Aleksandra and Mojović, Ljiljana",
year = "2023",
publisher = "National Technical University of Athens",
journal = "10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece",
title = "Fractionation of corncob biomass towards sustainable valorization in biorefinery processes",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6747"
}

Mladenović, D., Grbić, J., Đukić-Vuković, A.,& Mojović, L.. (2023). Fractionation of corncob biomass towards sustainable valorization in biorefinery processes. in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece
National Technical University of Athens..
https://hdl.handle.net/21.15107/rcub_technorep_6747

Mladenović D, Grbić J, Đukić-Vuković A, Mojović L. Fractionation of corncob biomass towards sustainable valorization in biorefinery processes. in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6747 .

Mladenović, Dragana, Grbić, Jovana, Đukić-Vuković, Aleksandra, Mojović, Ljiljana, "Fractionation of corncob biomass towards sustainable valorization in biorefinery processes" in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6747 .

TY  - JOUR
AU  - Grbić, Jovana
AU  - Mladenović, Dragana
AU  - Pavlović, Stefan
AU  - Lazović, Saša
AU  - Mojović, Ljiljana
AU  - Đukić-Vuković, Aleksandra
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5651
AB  - The depletion of fossil fuel reserves and severe environmental damage, resulting in climate change and global warming lead to a necessary shift to alternative renewable resources. Lignocellulose is a valuable feedstock for biorefineries, but its conversion is hindered by the limitations of conventional pretreatments lacking selectivity. This step should also be cost-effective and sustainable. In this paper, combined advanced oxidation techniques were applied for the treatment of corn stalks as a pretreatment for enzymatic hydrolysis. Their effect on delignification and cellulose digestibility was monitored. The combined non-thermal plasma/Fenton treatment appears to be suitable for breaking the complex lignocellulose structure, with a lignin content decrease of 39%. This treatment enhanced carbohydrate hydrolysis, resulting in 2.25 times increase in hexose yield, compared to the untreated sample. Long-term plasma treatment positively affected the textural properties, total porosity, and pore size diameter, of lignocellulose biomass. It enables the creation of materials with a stable system of pores and channels, for the unhindered diffusion of large organic molecules such as enzymes. Additionally, a combination of plasma treatment with Fenton reaction increased selectivity towards lignin degradation in comparison to independently applied Fenton treatment. This could be a significant advantage for a bottleneck in the current valorization of biomass.
PB  - Elsevier B.V.
T2  - Sustainable Chemistry and Pharmacy
T1  - Advanced oxidation processes in the treatment of corn stalks
SP  - 100962
VL  - 32
DO  - 10.1016/j.scp.2022.100962
ER  - 

@article{
author = "Grbić, Jovana and Mladenović, Dragana and Pavlović, Stefan and Lazović, Saša and Mojović, Ljiljana and Đukić-Vuković, Aleksandra",
year = "2023",
abstract = "The depletion of fossil fuel reserves and severe environmental damage, resulting in climate change and global warming lead to a necessary shift to alternative renewable resources. Lignocellulose is a valuable feedstock for biorefineries, but its conversion is hindered by the limitations of conventional pretreatments lacking selectivity. This step should also be cost-effective and sustainable. In this paper, combined advanced oxidation techniques were applied for the treatment of corn stalks as a pretreatment for enzymatic hydrolysis. Their effect on delignification and cellulose digestibility was monitored. The combined non-thermal plasma/Fenton treatment appears to be suitable for breaking the complex lignocellulose structure, with a lignin content decrease of 39%. This treatment enhanced carbohydrate hydrolysis, resulting in 2.25 times increase in hexose yield, compared to the untreated sample. Long-term plasma treatment positively affected the textural properties, total porosity, and pore size diameter, of lignocellulose biomass. It enables the creation of materials with a stable system of pores and channels, for the unhindered diffusion of large organic molecules such as enzymes. Additionally, a combination of plasma treatment with Fenton reaction increased selectivity towards lignin degradation in comparison to independently applied Fenton treatment. This could be a significant advantage for a bottleneck in the current valorization of biomass.",
publisher = "Elsevier B.V.",
journal = "Sustainable Chemistry and Pharmacy",
title = "Advanced oxidation processes in the treatment of corn stalks",
pages = "100962",
volume = "32",
doi = "10.1016/j.scp.2022.100962"
}

Grbić, J., Mladenović, D., Pavlović, S., Lazović, S., Mojović, L.,& Đukić-Vuković, A.. (2023). Advanced oxidation processes in the treatment of corn stalks. in Sustainable Chemistry and Pharmacy
Elsevier B.V.., 32, 100962.
https://doi.org/10.1016/j.scp.2022.100962

Grbić J, Mladenović D, Pavlović S, Lazović S, Mojović L, Đukić-Vuković A. Advanced oxidation processes in the treatment of corn stalks. in Sustainable Chemistry and Pharmacy. 2023;32:100962.
doi:10.1016/j.scp.2022.100962 .

Grbić, Jovana, Mladenović, Dragana, Pavlović, Stefan, Lazović, Saša, Mojović, Ljiljana, Đukić-Vuković, Aleksandra, "Advanced oxidation processes in the treatment of corn stalks" in Sustainable Chemistry and Pharmacy, 32 (2023):100962,
https://doi.org/10.1016/j.scp.2022.100962 . .

TY  - CONF
AU  - Grbić, Jovana
AU  - Bogdanović, Mihajlo
AU  - Mladenović, Dragana
AU  - Lazović, Saša
AU  - Mojović, Ljiljana
AU  - Đukić-Vuković, Aleksandra
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6345
AB  - To provide food with a longer shelf life, synthetic antioxidants are frequently used in the food industry. Common synthetic antioxidants includebutylated hydroxyanisole(BHA), butylated hydroxytoluene (BHT), propyl gallate (PG), and tert-butylated hydroquinone (TBHQ). Since those compounds are suspected to cause some health problems, such as liver damage, there is a strong initiative to develop and utilizeantioxidantsfrom natural origins.Medicinal plants could be a good alternative source of these compounds. Besides antioxidative properties, natural compounds derived from medicinal plants often have various biological activities, like anti-inflammatory, antimicrobial, analgesic, etc.These compounds can contribute to food's functional and technological properties and interact with food microbiota. In this study, aqueous extracts of tea production residues of common nettle (Urtica dioica L.), St. John’s wort (Hypericum perforatum L.), and hoary willowherb(Epilobium parviflorum L.) were treated with non-thermal plasma. The effect of different durations of plasma treatment on the antioxidative capacity of these extracts was monitored. The fermentation of these extracts solely and extracts supplemented with MRS broth by Ligilactobacillus salivarius was examined. Antioxidative properties of plasma-treated extracts were analyzed using DPPH and ABTS methods. The total phenol content of these extracts was determined by the Folin-Ciocalteu reagent. Generally, hoary willowherb extracts were rich in total phenol content, two or three orders of magnitude richer than the other two plants. They also showed significantly higher antioxidative activity. This activity slightly decreased with prolonged plasma treatment. However, that didn’t affect bacterial growth in these extracts. It was shown that L. salivarius growth was enhanced in the pure plasma-activated extracts of hoary willowherbin comparison to extracts supplemented with MRS broth. Though, the observed effects are substrate-dependent and vary from plant to plant. The antioxidative capacity of common nettle extracts remained intact during the plasma treatment. With the addition of MRS broth (25% v/v), bacterial growth was completely unhindered, providing approximately 109 CFU/ml during the exponential phase. Moreover, the 10-minute-long plasma treatment was effective in the inactivation of microorganisms already present in herbal biomass, preventing the spoilage of treated extracts. This could be an important step up in extraction by hurdle technology, especially in the production of symbiotic preparations possessing both antioxidative and probiotic properties.
PB  - University of East Sarajevo Faculty of Technology
C3  - Book of abstracts VIII International congress Engineering, environment and materials in process industry
T1  - Effect of non-thermal plasma treatment on antioxidative and prebiotic properties of aqueous herbal extracts
SP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6345
ER  - 

@conference{
author = "Grbić, Jovana and Bogdanović, Mihajlo and Mladenović, Dragana and Lazović, Saša and Mojović, Ljiljana and Đukić-Vuković, Aleksandra",
year = "2023",
abstract = "To provide food with a longer shelf life, synthetic antioxidants are frequently used in the food industry. Common synthetic antioxidants includebutylated hydroxyanisole(BHA), butylated hydroxytoluene (BHT), propyl gallate (PG), and tert-butylated hydroquinone (TBHQ). Since those compounds are suspected to cause some health problems, such as liver damage, there is a strong initiative to develop and utilizeantioxidantsfrom natural origins.Medicinal plants could be a good alternative source of these compounds. Besides antioxidative properties, natural compounds derived from medicinal plants often have various biological activities, like anti-inflammatory, antimicrobial, analgesic, etc.These compounds can contribute to food's functional and technological properties and interact with food microbiota. In this study, aqueous extracts of tea production residues of common nettle (Urtica dioica L.), St. John’s wort (Hypericum perforatum L.), and hoary willowherb(Epilobium parviflorum L.) were treated with non-thermal plasma. The effect of different durations of plasma treatment on the antioxidative capacity of these extracts was monitored. The fermentation of these extracts solely and extracts supplemented with MRS broth by Ligilactobacillus salivarius was examined. Antioxidative properties of plasma-treated extracts were analyzed using DPPH and ABTS methods. The total phenol content of these extracts was determined by the Folin-Ciocalteu reagent. Generally, hoary willowherb extracts were rich in total phenol content, two or three orders of magnitude richer than the other two plants. They also showed significantly higher antioxidative activity. This activity slightly decreased with prolonged plasma treatment. However, that didn’t affect bacterial growth in these extracts. It was shown that L. salivarius growth was enhanced in the pure plasma-activated extracts of hoary willowherbin comparison to extracts supplemented with MRS broth. Though, the observed effects are substrate-dependent and vary from plant to plant. The antioxidative capacity of common nettle extracts remained intact during the plasma treatment. With the addition of MRS broth (25% v/v), bacterial growth was completely unhindered, providing approximately 109 CFU/ml during the exponential phase. Moreover, the 10-minute-long plasma treatment was effective in the inactivation of microorganisms already present in herbal biomass, preventing the spoilage of treated extracts. This could be an important step up in extraction by hurdle technology, especially in the production of symbiotic preparations possessing both antioxidative and probiotic properties.",
publisher = "University of East Sarajevo Faculty of Technology",
journal = "Book of abstracts VIII International congress Engineering, environment and materials in process industry",
title = "Effect of non-thermal plasma treatment on antioxidative and prebiotic properties of aqueous herbal extracts",
pages = "155",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6345"
}

Grbić, J., Bogdanović, M., Mladenović, D., Lazović, S., Mojović, L.,& Đukić-Vuković, A.. (2023). Effect of non-thermal plasma treatment on antioxidative and prebiotic properties of aqueous herbal extracts. in Book of abstracts VIII International congress Engineering, environment and materials in process industry
University of East Sarajevo Faculty of Technology., 155.
https://hdl.handle.net/21.15107/rcub_technorep_6345

Grbić J, Bogdanović M, Mladenović D, Lazović S, Mojović L, Đukić-Vuković A. Effect of non-thermal plasma treatment on antioxidative and prebiotic properties of aqueous herbal extracts. in Book of abstracts VIII International congress Engineering, environment and materials in process industry. 2023;:155.
https://hdl.handle.net/21.15107/rcub_technorep_6345 .

Grbić, Jovana, Bogdanović, Mihajlo, Mladenović, Dragana, Lazović, Saša, Mojović, Ljiljana, Đukić-Vuković, Aleksandra, "Effect of non-thermal plasma treatment on antioxidative and prebiotic properties of aqueous herbal extracts" in Book of abstracts VIII International congress Engineering, environment and materials in process industry (2023):155,
https://hdl.handle.net/21.15107/rcub_technorep_6345 .

TY  - CONF
AU  - Đukić-Vuković, Aleksandra
AU  - Grbić, Jovana
AU  - Mladenović, Dragana
AU  - Pavlović, Stefan
AU  - Lazović, Saša
AU  - Mojović, Ljiljana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6745
PB  - National Technical University of Athens
C3  - 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece
T1  - Corn stalks as a lignocellulose substrate for biorefinery applications
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6745
ER  - 

@conference{
author = "Đukić-Vuković, Aleksandra and Grbić, Jovana and Mladenović, Dragana and Pavlović, Stefan and Lazović, Saša and Mojović, Ljiljana",
year = "2023",
publisher = "National Technical University of Athens",
journal = "10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece",
title = "Corn stalks as a lignocellulose substrate for biorefinery applications",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6745"
}

Đukić-Vuković, A., Grbić, J., Mladenović, D., Pavlović, S., Lazović, S.,& Mojović, L.. (2023). Corn stalks as a lignocellulose substrate for biorefinery applications. in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece
National Technical University of Athens..
https://hdl.handle.net/21.15107/rcub_technorep_6745

Đukić-Vuković A, Grbić J, Mladenović D, Pavlović S, Lazović S, Mojović L. Corn stalks as a lignocellulose substrate for biorefinery applications. in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6745 .

Đukić-Vuković, Aleksandra, Grbić, Jovana, Mladenović, Dragana, Pavlović, Stefan, Lazović, Saša, Mojović, Ljiljana, "Corn stalks as a lignocellulose substrate for biorefinery applications" in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6745 .

TY  - CONF
AU  - Mladenović, Mihailo
AU  - Bogdanović, Mihajlo
AU  - Mladenović, Dragana
AU  - Đukić-Vuković, Aleksandra
AU  - Mojović, Ljiljana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6744
PB  - National Technical University of Athens
C3  - 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece
T1  - Common nettle processing residues as a valuable source of antioxidants
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6744
ER  - 

@conference{
author = "Mladenović, Mihailo and Bogdanović, Mihajlo and Mladenović, Dragana and Đukić-Vuković, Aleksandra and Mojović, Ljiljana",
year = "2023",
publisher = "National Technical University of Athens",
journal = "10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece",
title = "Common nettle processing residues as a valuable source of antioxidants",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6744"
}

Mladenović, M., Bogdanović, M., Mladenović, D., Đukić-Vuković, A.,& Mojović, L.. (2023). Common nettle processing residues as a valuable source of antioxidants. in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece
National Technical University of Athens..
https://hdl.handle.net/21.15107/rcub_technorep_6744

Mladenović M, Bogdanović M, Mladenović D, Đukić-Vuković A, Mojović L. Common nettle processing residues as a valuable source of antioxidants. in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6744 .

Mladenović, Mihailo, Bogdanović, Mihajlo, Mladenović, Dragana, Đukić-Vuković, Aleksandra, Mojović, Ljiljana, "Common nettle processing residues as a valuable source of antioxidants" in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6744 .

TY  - CONF
AU  - Mojović, Ljiljana
AU  - Jović, Jelena
AU  - Mladenović, Dragana
AU  - Đukić-Vuković, Aleksandra
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6245
AB  - Lignocellulosic biomass has become one of the most explored renewable substrates for the production of many valuable chemicals, biofuels, and food or feed ingredients or products. However, a complex structure blocs its direct utilization and directs to utilization of particular pretreatments in order to remove lignin and facilitate the biomass hydrolysis prior to its further processing. The pretreatments could be done by chemical, physical, physicochemical means, or using biological methods. Biological methods involving the use of fungi or their enzymes in the pretreatment and hydrolysis of the lignocellulosic substrate are ecologically friendly, rather time consuming, but with no formation of harmless components that can inhibit the production microorganism (Nair and Sivakumar, 2020). The limitations, such as process longevity and loss of valuable sugars, can be alleviated or overcome by utilization of novel efficient microorganisms, e.g. fungi which are selective biomass decomposers, as well as by optimizing conditions of the pretreatment. In this study, a potential use of novel isolated Serbian autochthonous fungi in the pretreatment of lignocellulosic biomass was studied. Isolates were collected in the southern Serbia near the City of Leskovac. The strains were isolated from stumps, fallen trees and branches, fallen leaves, or living trees found in the oak forest and orchards around the forest. Among 12 isolated fungi, the isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 stood out as ligninolytic enzyme producers and were selected for potential use in the pretreatment of waste lignocellulosic biomass (beechwood sawdust). Table 1. shows biomass and lignin reduction and selectivity coefficients relative to the total lignin reduction (selectivity coefficient 1), and relative to the Klason’s lignin reduction (selectivity coefficient 2) after 35 days of incubation under non optimized conditions. More lignin was degraded by T. hirsuta F13 than S. gausapatum F28: its selectivity coefficient, relative to the total lignin, was 1.7, while the selectivity coefficient obtained for S. gausapatum was 1.1. The values of the selectivity coefficient, relative to Klason's lignin, were 1.47 for the isolate F13 and 0.8 for the isolate F28, which confirmed that T. hirsuta F13 was the best candidate among the isolated fungi for use for the biomass pretreatment. An isolate identified as Myrmaecium fulvopruinatum F14 showed high hydrolytic activity, but negligible ligninolytic activity, and was selected as a potential producer of important industrial hydrolytic enzymes. The three novel fungal isolates with pronounced lignocellulolytic activities were identified using ITS sequences, and the sequences were deposited in the NCBI GenBank database. Their accession numbers are KY264754.1 (Trametes hirsuta F13), KY264753.1 (Stereum gausapatum F28), and MF521930.1 (Myrmaecium fulvopruinatum F28) (Jović et al., 2018). The isolate T. hirsuta F13 which was selected as the best candidate for the pretreatment of lignocelulosic biomass was further studied in order to enhance its biomass degrading efficiency by optimizing the pretreatment conditions. It was found that the supplementation with sugar beet molasses stillage (MS) could significantly improve the biological pretreatment. The initial results showed that the addition of MS could improve ligninolytic activity, but also that the type of a dominant enzyme activity differed depending on the stillage concentration. Besides, other pretreatment parameters such as substrate moisture and temperature of the treatment were found important. The results have shown that the use of MS at the concentration of 13% as a supplement at the temperature of 25 0C and substrate moisture of 63% can shorten the time of the biological Monitored parameter Fungal isolate T. hirshuta F13 S.gauspatum F28 Biomass reduction (%) 19 24 Total lignin reduction (%) 33.8 28 Selectivity coefficient 1 1.7 1.1 Klason's lignin reduction (%) 28 19 Selectivity coefficient 2 1.47 0.8 pretreatment from 35 to 18 days, while the selectivity of biomass degradation was also improved of (Jović et al., 2020). Finally, this study examined and selected three novel autochthones fungal isolates, T. hirsuta F13, M. fulvopruinatum F14, and S. gausapatum F28, which can be used in the production of industrially important lignocellulolytic enzymes and/or in biomass pretreatment under determined conditions.
PB  - NTUA
C3  - 9th International Conference on Sustainable Solid Waste Management
T1  - Novel autochthonous fungi for the treatment of lignocellulosic biomass
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6245
ER  - 

@conference{
author = "Mojović, Ljiljana and Jović, Jelena and Mladenović, Dragana and Đukić-Vuković, Aleksandra",
year = "2022",
abstract = "Lignocellulosic biomass has become one of the most explored renewable substrates for the production of many valuable chemicals, biofuels, and food or feed ingredients or products. However, a complex structure blocs its direct utilization and directs to utilization of particular pretreatments in order to remove lignin and facilitate the biomass hydrolysis prior to its further processing. The pretreatments could be done by chemical, physical, physicochemical means, or using biological methods. Biological methods involving the use of fungi or their enzymes in the pretreatment and hydrolysis of the lignocellulosic substrate are ecologically friendly, rather time consuming, but with no formation of harmless components that can inhibit the production microorganism (Nair and Sivakumar, 2020). The limitations, such as process longevity and loss of valuable sugars, can be alleviated or overcome by utilization of novel efficient microorganisms, e.g. fungi which are selective biomass decomposers, as well as by optimizing conditions of the pretreatment. In this study, a potential use of novel isolated Serbian autochthonous fungi in the pretreatment of lignocellulosic biomass was studied. Isolates were collected in the southern Serbia near the City of Leskovac. The strains were isolated from stumps, fallen trees and branches, fallen leaves, or living trees found in the oak forest and orchards around the forest. Among 12 isolated fungi, the isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 stood out as ligninolytic enzyme producers and were selected for potential use in the pretreatment of waste lignocellulosic biomass (beechwood sawdust). Table 1. shows biomass and lignin reduction and selectivity coefficients relative to the total lignin reduction (selectivity coefficient 1), and relative to the Klason’s lignin reduction (selectivity coefficient 2) after 35 days of incubation under non optimized conditions. More lignin was degraded by T. hirsuta F13 than S. gausapatum F28: its selectivity coefficient, relative to the total lignin, was 1.7, while the selectivity coefficient obtained for S. gausapatum was 1.1. The values of the selectivity coefficient, relative to Klason's lignin, were 1.47 for the isolate F13 and 0.8 for the isolate F28, which confirmed that T. hirsuta F13 was the best candidate among the isolated fungi for use for the biomass pretreatment. An isolate identified as Myrmaecium fulvopruinatum F14 showed high hydrolytic activity, but negligible ligninolytic activity, and was selected as a potential producer of important industrial hydrolytic enzymes. The three novel fungal isolates with pronounced lignocellulolytic activities were identified using ITS sequences, and the sequences were deposited in the NCBI GenBank database. Their accession numbers are KY264754.1 (Trametes hirsuta F13), KY264753.1 (Stereum gausapatum F28), and MF521930.1 (Myrmaecium fulvopruinatum F28) (Jović et al., 2018). The isolate T. hirsuta F13 which was selected as the best candidate for the pretreatment of lignocelulosic biomass was further studied in order to enhance its biomass degrading efficiency by optimizing the pretreatment conditions. It was found that the supplementation with sugar beet molasses stillage (MS) could significantly improve the biological pretreatment. The initial results showed that the addition of MS could improve ligninolytic activity, but also that the type of a dominant enzyme activity differed depending on the stillage concentration. Besides, other pretreatment parameters such as substrate moisture and temperature of the treatment were found important. The results have shown that the use of MS at the concentration of 13% as a supplement at the temperature of 25 0C and substrate moisture of 63% can shorten the time of the biological Monitored parameter Fungal isolate T. hirshuta F13 S.gauspatum F28 Biomass reduction (%) 19 24 Total lignin reduction (%) 33.8 28 Selectivity coefficient 1 1.7 1.1 Klason's lignin reduction (%) 28 19 Selectivity coefficient 2 1.47 0.8 pretreatment from 35 to 18 days, while the selectivity of biomass degradation was also improved of (Jović et al., 2020). Finally, this study examined and selected three novel autochthones fungal isolates, T. hirsuta F13, M. fulvopruinatum F14, and S. gausapatum F28, which can be used in the production of industrially important lignocellulolytic enzymes and/or in biomass pretreatment under determined conditions.",
publisher = "NTUA",
journal = "9th International Conference on Sustainable Solid Waste Management",
title = "Novel autochthonous fungi for the treatment of lignocellulosic biomass",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6245"
}

Mojović, L., Jović, J., Mladenović, D.,& Đukić-Vuković, A.. (2022). Novel autochthonous fungi for the treatment of lignocellulosic biomass. in 9th International Conference on Sustainable Solid Waste Management
NTUA..
https://hdl.handle.net/21.15107/rcub_technorep_6245

Mojović L, Jović J, Mladenović D, Đukić-Vuković A. Novel autochthonous fungi for the treatment of lignocellulosic biomass. in 9th International Conference on Sustainable Solid Waste Management. 2022;.
https://hdl.handle.net/21.15107/rcub_technorep_6245 .

Mojović, Ljiljana, Jović, Jelena, Mladenović, Dragana, Đukić-Vuković, Aleksandra, "Novel autochthonous fungi for the treatment of lignocellulosic biomass" in 9th International Conference on Sustainable Solid Waste Management (2022),
https://hdl.handle.net/21.15107/rcub_technorep_6245 .

TY  - JOUR
AU  - Grbić, Jovana
AU  - Đukić-Vuković, Aleksandra
AU  - Mladenović, Dragana
AU  - Lazović, Saša
AU  - Mojović, Ljiljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6104
AB  - Lignocellulosic biomass is a cheap raw material that, thanks to its high carbohydrate content, can be used in fermentation to
produce biofuels, biogas and other compounds. Its complex structure, including cellulose, hemicellulose and lignin, requires prior
treatment of the biomass to facilitate hydrolysis to simple sugars. Today, biomass is only partially utilized and generates about 14%
of the world´s energy. This is because the most commonly used physical, chemical and physicochemical treatments are not
sustainable. They are energy-consuming but still low in productivity and toxic inhibitors formed during these treatments could hinder
later steps of fermentation.Biomass treatment with advanced oxidation techniques has great potential as an environmentally friendly,
so-called "green" treatment. These processes generate reactive species (radicals, electrons, ions and peroxides) that attack cellulose,
hemicellulose, and lignin components. In this work, the effects of non-thermal plasma, the Fenton process, and the combined
treatment of corn stalks with non-thermal plasma/Fenton were compared. Grounded biomass of corn stalks was mixed with Fenton
reagent and hydrogen peroxide at different ratios and subjected to non-thermal plasma treatment. Carbohydrate content was
decreased in non-thermal plasma treated samples both with and without Fe2+. However, a specific biomass: Fe2+:H2O2 ratio was
required to achieve the highest rate of lignocellulose decomposition. The cellulose and hemicellulose fractions were affected and
reduced by the treatments studied but resulted in almost no changes in the cellulose crystallinity index. The lower lignin content and
cellulose crystallinity allow for more efficient enzyme hydrolysis of the treated lignocellulose and new options for valorization in
fermentations.
AB  - Lignocelulozna biomasa predstavlja jeftinu sirovinu koja se može koristiti u fermentacionim procesima za dobijanje biogoriva,
biogasa i drugih jedinjenja, zahvaljujući visokom sadržaju ugljenih hidrata. Složena struktura, koja uključuje celulozu, hemicelulozu
i lignin, zahteva prethodni tretman biomase kojim se olakšava hidroliza do prostih šećera. Danas se biomasa samo delimično
eksploatiše i generiše oko 14% energije na svetskom nivou. To je prevashodno zbog male održivosti najčešće korišćenih fizičkih,
hemijskih i fizičko-hemijskih tretmana. Ovi procesi troše veliku količinu energije, imaju malu produktivnost, a toksični sporedni
proizvodi koji nastaju tokom tretmana mogu ometati kasnije korake fermentacije. Tretman biomase naprednim oksidacionim
procesima ima veliki potencijal kao ekološki prihvatljiv, tzv. „zeleni“ tretman. Tokom ovog procesa dolazi do stvaranja reaktivnih
vrsta (radikala, elektrona, jona i peroksida), koje napadaju celulozu, hemicelulozu i lignin. U ovom radu upoređeni su efekti
tretmana kukuruzne stabljike netermalnom plazmom, Fentonovim reagensom i kombinovanog tretmana netermalnom
plazmom/Fenton reagensom. Samlevena biomasa kukuruzne stabljike pomešana je sa Fentonovim reagensom i vodonik peroksidom u
različitim odnosima, a zatim je podvrgnuta tretmanu netermalnom plazmom. Sadržaj celuloze i hemiceluloze je značajno smanjen u
uzorcima tretiranim netermalnom plazmom i u prisustvu i u odsustvu Fe2+. Ipak, najveći stepen redukcije lignoceluloze je postignut
pri određenom odnosu biomasa:Fe2+:vodonik peroksid. Primenjeni tretmani su uticali i na hemiceluloznu frakciju, ostavljajući indeks
kristaliničnosti celuloze skoro nepromenjenim. Niži sadržaj lignina i manji indeks kristaliničnosti celuloze omogućavaju efikasniju
enzimsku hidrolizu tretirane lignoceluloze i nove načine za valorizaciju u fermentacionim procesima.
T2  - Journal on Processing and Energy in Agriculture
T1  - EFFECT OF NON-THERMAL PLASMA ON CELLULOSE CRYSTALLINITY AND LIGNIN CONTENT IN CORN STALKS
T1  - UTICAJ TRETMANA NETERMALNOM PLAZMOM NA KRISTALINIČNOST CELULOZE I SADRŽAJ LIGNINA U KUKURUZNOJ STABLJICI
EP  - 56
IS  - 2
SP  - 52
VL  - 26
DO  - 10.5937/jpea26-36871
ER  - 

@article{
author = "Grbić, Jovana and Đukić-Vuković, Aleksandra and Mladenović, Dragana and Lazović, Saša and Mojović, Ljiljana",
year = "2022",
abstract = "Lignocellulosic biomass is a cheap raw material that, thanks to its high carbohydrate content, can be used in fermentation to
produce biofuels, biogas and other compounds. Its complex structure, including cellulose, hemicellulose and lignin, requires prior
treatment of the biomass to facilitate hydrolysis to simple sugars. Today, biomass is only partially utilized and generates about 14%
of the world´s energy. This is because the most commonly used physical, chemical and physicochemical treatments are not
sustainable. They are energy-consuming but still low in productivity and toxic inhibitors formed during these treatments could hinder
later steps of fermentation.Biomass treatment with advanced oxidation techniques has great potential as an environmentally friendly,
so-called "green" treatment. These processes generate reactive species (radicals, electrons, ions and peroxides) that attack cellulose,
hemicellulose, and lignin components. In this work, the effects of non-thermal plasma, the Fenton process, and the combined
treatment of corn stalks with non-thermal plasma/Fenton were compared. Grounded biomass of corn stalks was mixed with Fenton
reagent and hydrogen peroxide at different ratios and subjected to non-thermal plasma treatment. Carbohydrate content was
decreased in non-thermal plasma treated samples both with and without Fe2+. However, a specific biomass: Fe2+:H2O2 ratio was
required to achieve the highest rate of lignocellulose decomposition. The cellulose and hemicellulose fractions were affected and
reduced by the treatments studied but resulted in almost no changes in the cellulose crystallinity index. The lower lignin content and
cellulose crystallinity allow for more efficient enzyme hydrolysis of the treated lignocellulose and new options for valorization in
fermentations., Lignocelulozna biomasa predstavlja jeftinu sirovinu koja se može koristiti u fermentacionim procesima za dobijanje biogoriva,
biogasa i drugih jedinjenja, zahvaljujući visokom sadržaju ugljenih hidrata. Složena struktura, koja uključuje celulozu, hemicelulozu
i lignin, zahteva prethodni tretman biomase kojim se olakšava hidroliza do prostih šećera. Danas se biomasa samo delimično
eksploatiše i generiše oko 14% energije na svetskom nivou. To je prevashodno zbog male održivosti najčešće korišćenih fizičkih,
hemijskih i fizičko-hemijskih tretmana. Ovi procesi troše veliku količinu energije, imaju malu produktivnost, a toksični sporedni
proizvodi koji nastaju tokom tretmana mogu ometati kasnije korake fermentacije. Tretman biomase naprednim oksidacionim
procesima ima veliki potencijal kao ekološki prihvatljiv, tzv. „zeleni“ tretman. Tokom ovog procesa dolazi do stvaranja reaktivnih
vrsta (radikala, elektrona, jona i peroksida), koje napadaju celulozu, hemicelulozu i lignin. U ovom radu upoređeni su efekti
tretmana kukuruzne stabljike netermalnom plazmom, Fentonovim reagensom i kombinovanog tretmana netermalnom
plazmom/Fenton reagensom. Samlevena biomasa kukuruzne stabljike pomešana je sa Fentonovim reagensom i vodonik peroksidom u
različitim odnosima, a zatim je podvrgnuta tretmanu netermalnom plazmom. Sadržaj celuloze i hemiceluloze je značajno smanjen u
uzorcima tretiranim netermalnom plazmom i u prisustvu i u odsustvu Fe2+. Ipak, najveći stepen redukcije lignoceluloze je postignut
pri određenom odnosu biomasa:Fe2+:vodonik peroksid. Primenjeni tretmani su uticali i na hemiceluloznu frakciju, ostavljajući indeks
kristaliničnosti celuloze skoro nepromenjenim. Niži sadržaj lignina i manji indeks kristaliničnosti celuloze omogućavaju efikasniju
enzimsku hidrolizu tretirane lignoceluloze i nove načine za valorizaciju u fermentacionim procesima.",
journal = "Journal on Processing and Energy in Agriculture",
title = "EFFECT OF NON-THERMAL PLASMA ON CELLULOSE CRYSTALLINITY AND LIGNIN CONTENT IN CORN STALKS, UTICAJ TRETMANA NETERMALNOM PLAZMOM NA KRISTALINIČNOST CELULOZE I SADRŽAJ LIGNINA U KUKURUZNOJ STABLJICI",
pages = "56-52",
number = "2",
volume = "26",
doi = "10.5937/jpea26-36871"
}

Grbić, J., Đukić-Vuković, A., Mladenović, D., Lazović, S.,& Mojović, L.. (2022). EFFECT OF NON-THERMAL PLASMA ON CELLULOSE CRYSTALLINITY AND LIGNIN CONTENT IN CORN STALKS. in Journal on Processing and Energy in Agriculture, 26(2), 52-56.
https://doi.org/10.5937/jpea26-36871

Grbić J, Đukić-Vuković A, Mladenović D, Lazović S, Mojović L. EFFECT OF NON-THERMAL PLASMA ON CELLULOSE CRYSTALLINITY AND LIGNIN CONTENT IN CORN STALKS. in Journal on Processing and Energy in Agriculture. 2022;26(2):52-56.
doi:10.5937/jpea26-36871 .

Grbić, Jovana, Đukić-Vuković, Aleksandra, Mladenović, Dragana, Lazović, Saša, Mojović, Ljiljana, "EFFECT OF NON-THERMAL PLASMA ON CELLULOSE CRYSTALLINITY AND LIGNIN CONTENT IN CORN STALKS" in Journal on Processing and Energy in Agriculture, 26, no. 2 (2022):52-56,
https://doi.org/10.5937/jpea26-36871 . .

TY  - JOUR
AU  - Grbić, Jovana
AU  - Đukić-Vuković, Aleksandra
AU  - Mladenović, Dragana
AU  - Lazović, Saša
AU  - Mojović, Ljiljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6151
AB  - Lignocellulosic  biomass  is  a  cheap  raw  material  that,  thanks  to  its  high  carbohydrate  content,  can  be  used  in  fermentation  to  produce  biofuels,  biogas  and  other  compounds.  Its  complex  structure,  including  cellulose,  hemicellulose  and  lignin,  requires  prior treatment of the biomass to facilitate hydrolysis to simple sugars. Today, biomass is only partially utilized and generates about 14% of  the  world ́s  energy.  This  is  because  the  most  commonly  used  physical,  chemical  and  physicochemical  treatments  are  not  sustainable. They are energy-consuming but still low in productivity and toxic inhibitors formed during these treatments could hinder later steps of fermentation. Biomass treatment with advanced oxidation techniques has great potential as an environmentally friendly, so-called "green" treatment. These processes generate reactive species (radicals, electrons, ions and peroxides) that attack cellulose, hemicellulose,  and  lignin  components.  In  this  work,  the  effects  of  non-thermal  plasma,  the  Fenton  process,  and  the  combined  treatment of corn stalks with non-thermal plasma/Fenton were compared. Grounded biomass of corn stalks was mixed with Fenton reagent  and  hydrogen  peroxide  at  different  ratios  and  subjected  to  non-thermal  plasma  treatment.  Carbohydrate  content  was  decreased  in  non-thermal  plasma  treated  samples  both  with  and  without  Fe2+.  However, a specific  biomass:  Fe2+:H2O2  ratio  was  required  to  achieve  the  highest  rate  of  lignocellulose  decomposition.  The  cellulose and  hemicellulose  fractions  were  affected  and  reduced by the treatments studied but resulted in almost no changes in the cellulose crystallinity index. The lower lignin content and cellulose  crystallinity  allow  for  more  efficient  enzyme  hydrolysis  of  the  treated  lignocellulose  and  new  options  for  valorization  in  fermentations.
AB  - Lignocelulozna  biomasa  predstavlja  jeftinu  sirovinu  koja  se  može  koristiti  u  fermentacionim  procesima  za  dobijanje  biogoriva,  biogasa i drugih jedinjenja, zahvaljujući visokom sadržaju ugljenih hidrata. Složena struktura, koja uključuje celulozu, hemicelulozu i  lignin,  zahteva  prethodni  tretman  biomase  kojim  se  olakšava  hidroliza  do  prostih  šećera.  Danas  se  biomasa  samo  delimično eksploatiše  i  generiše  oko  14%  energije  na  svetskom  nivou.  To  je  prevashodno  zbog  male  održivosti  najčešće  korišćenih  fizičkih, hemijskih  i  fizičko-hemijskih  tretmana.  Ovi  procesi  troše  veliku  količinu  energije,  imaju  malu  produktivnost,  a  toksični  sporedni  proizvodi  koji  nastaju  tokom  tretmana  mogu  ometati  kasnije  korake  fermentacije.  Tretman  biomase  naprednim  oksidacionim  procesima  ima  veliki  potencijal  kao  ekološki  prihvatljiv,  tzv.  „zeleni“  tretman.  Tokom  ovog  procesa  dolazi  do  stvaranja  reaktivnih vrsta  (radikala,  elektrona,  jona  i  peroksida),  koje  napadaju  celulozu,  hemicelulozu  i  lignin.  U  ovom  radu  upoređeni  su  efekti  tretmana    kukuruzne    stabljike    netermalnom    plazmom,    Fentonovim    reagensom    i    kombinovanog    tretmana    netermalnom    plazmom/Fentonreagensom.Samlevena biomasa kukuruzne stabljike pomešana je sa Fentonovim reagensom i vodonik peroksidom u različitim odnosima, a zatim je podvrgnuta tretmanu netermalnom plazmom. Sadržaj celuloze i hemiceluloze je značajno smanjen u uzorcima tretiranim netermalnom plazmom i u prisustvu i u odsustvu Fe2+. Ipak, najveći stepen redukcije lignoceluloze je postignut pri određenom odnosu biomasa:Fe2+:vodonik peroksid. Primenjeni tretmani su uticali i na hemiceluloznu frakciju, ostavljajući indeks kristaliničnosti  celuloze  skoro  nepromenjenim.  Niži  sadržaj  lignina  i  manji  indeks  kristaliničnosti  celuloze  omogućavaju  efikasniju  enzimsku hidrolizu tretirane lignoceluloze i nove načine za valorizaciju u fermentacionim procesima.
PB  - National Society of Processing and Energy in Agriculture, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - Effect of Non-thermal Plasma on Cellulose Crystallinity and Lignin Content in Corn Stalks
T1  - Uticaj tretmana netermalnom plazmom na kristaliničnost celuloze i sadržaj lignina u kukuruznoj stabljici
EP  - 56
IS  - 2
SP  - 52
VL  - 26
DO  - 10.5937/jpea26-36871
ER  - 

@article{
author = "Grbić, Jovana and Đukić-Vuković, Aleksandra and Mladenović, Dragana and Lazović, Saša and Mojović, Ljiljana",
year = "2022",
abstract = "Lignocellulosic  biomass  is  a  cheap  raw  material  that,  thanks  to  its  high  carbohydrate  content,  can  be  used  in  fermentation  to  produce  biofuels,  biogas  and  other  compounds.  Its  complex  structure,  including  cellulose,  hemicellulose  and  lignin,  requires  prior treatment of the biomass to facilitate hydrolysis to simple sugars. Today, biomass is only partially utilized and generates about 14% of  the  world ́s  energy.  This  is  because  the  most  commonly  used  physical,  chemical  and  physicochemical  treatments  are  not  sustainable. They are energy-consuming but still low in productivity and toxic inhibitors formed during these treatments could hinder later steps of fermentation. Biomass treatment with advanced oxidation techniques has great potential as an environmentally friendly, so-called "green" treatment. These processes generate reactive species (radicals, electrons, ions and peroxides) that attack cellulose, hemicellulose,  and  lignin  components.  In  this  work,  the  effects  of  non-thermal  plasma,  the  Fenton  process,  and  the  combined  treatment of corn stalks with non-thermal plasma/Fenton were compared. Grounded biomass of corn stalks was mixed with Fenton reagent  and  hydrogen  peroxide  at  different  ratios  and  subjected  to  non-thermal  plasma  treatment.  Carbohydrate  content  was  decreased  in  non-thermal  plasma  treated  samples  both  with  and  without  Fe2+.  However, a specific  biomass:  Fe2+:H2O2  ratio  was  required  to  achieve  the  highest  rate  of  lignocellulose  decomposition.  The  cellulose and  hemicellulose  fractions  were  affected  and  reduced by the treatments studied but resulted in almost no changes in the cellulose crystallinity index. The lower lignin content and cellulose  crystallinity  allow  for  more  efficient  enzyme  hydrolysis  of  the  treated  lignocellulose  and  new  options  for  valorization  in  fermentations., Lignocelulozna  biomasa  predstavlja  jeftinu  sirovinu  koja  se  može  koristiti  u  fermentacionim  procesima  za  dobijanje  biogoriva,  biogasa i drugih jedinjenja, zahvaljujući visokom sadržaju ugljenih hidrata. Složena struktura, koja uključuje celulozu, hemicelulozu i  lignin,  zahteva  prethodni  tretman  biomase  kojim  se  olakšava  hidroliza  do  prostih  šećera.  Danas  se  biomasa  samo  delimično eksploatiše  i  generiše  oko  14%  energije  na  svetskom  nivou.  To  je  prevashodno  zbog  male  održivosti  najčešće  korišćenih  fizičkih, hemijskih  i  fizičko-hemijskih  tretmana.  Ovi  procesi  troše  veliku  količinu  energije,  imaju  malu  produktivnost,  a  toksični  sporedni  proizvodi  koji  nastaju  tokom  tretmana  mogu  ometati  kasnije  korake  fermentacije.  Tretman  biomase  naprednim  oksidacionim  procesima  ima  veliki  potencijal  kao  ekološki  prihvatljiv,  tzv.  „zeleni“  tretman.  Tokom  ovog  procesa  dolazi  do  stvaranja  reaktivnih vrsta  (radikala,  elektrona,  jona  i  peroksida),  koje  napadaju  celulozu,  hemicelulozu  i  lignin.  U  ovom  radu  upoređeni  su  efekti  tretmana    kukuruzne    stabljike    netermalnom    plazmom,    Fentonovim    reagensom    i    kombinovanog    tretmana    netermalnom    plazmom/Fentonreagensom.Samlevena biomasa kukuruzne stabljike pomešana je sa Fentonovim reagensom i vodonik peroksidom u različitim odnosima, a zatim je podvrgnuta tretmanu netermalnom plazmom. Sadržaj celuloze i hemiceluloze je značajno smanjen u uzorcima tretiranim netermalnom plazmom i u prisustvu i u odsustvu Fe2+. Ipak, najveći stepen redukcije lignoceluloze je postignut pri određenom odnosu biomasa:Fe2+:vodonik peroksid. Primenjeni tretmani su uticali i na hemiceluloznu frakciju, ostavljajući indeks kristaliničnosti  celuloze  skoro  nepromenjenim.  Niži  sadržaj  lignina  i  manji  indeks  kristaliničnosti  celuloze  omogućavaju  efikasniju  enzimsku hidrolizu tretirane lignoceluloze i nove načine za valorizaciju u fermentacionim procesima.",
publisher = "National Society of Processing and Energy in Agriculture, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "Effect of Non-thermal Plasma on Cellulose Crystallinity and Lignin Content in Corn Stalks, Uticaj tretmana netermalnom plazmom na kristaliničnost celuloze i sadržaj lignina u kukuruznoj stabljici",
pages = "56-52",
number = "2",
volume = "26",
doi = "10.5937/jpea26-36871"
}

Grbić, J., Đukić-Vuković, A., Mladenović, D., Lazović, S.,& Mojović, L.. (2022). Effect of Non-thermal Plasma on Cellulose Crystallinity and Lignin Content in Corn Stalks. in Journal on Processing and Energy in Agriculture
National Society of Processing and Energy in Agriculture, Novi Sad., 26(2), 52-56.
https://doi.org/10.5937/jpea26-36871

Grbić J, Đukić-Vuković A, Mladenović D, Lazović S, Mojović L. Effect of Non-thermal Plasma on Cellulose Crystallinity and Lignin Content in Corn Stalks. in Journal on Processing and Energy in Agriculture. 2022;26(2):52-56.
doi:10.5937/jpea26-36871 .

Grbić, Jovana, Đukić-Vuković, Aleksandra, Mladenović, Dragana, Lazović, Saša, Mojović, Ljiljana, "Effect of Non-thermal Plasma on Cellulose Crystallinity and Lignin Content in Corn Stalks" in Journal on Processing and Energy in Agriculture, 26, no. 2 (2022):52-56,
https://doi.org/10.5937/jpea26-36871 . .

TY  - CHAP
AU  - Đukić-Vuković, Aleksandra P.
AU  - Mladenović, Dragana D.
AU  - Pejin, Jelena D.
AU  - Mojović, Ljiljana V.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6152
AB  - Food waste and by-products are generated in all stages of food supply chain and it is estimated that third of all produced food is wasted. The goal is to minimise avoidable waste, to recover resources in sustainable way and to recycle them back into the food chain. A bibliometric mapping is used to analyse state of the art in non-thermal processing of food waste and by-products. Maximizing product yield, lowering energy consumption, economical, and environmental aspects are essential criteria for designing a new sustainable biorefinery process on food wastes and by-products. Many compounds important for food industry could be recovered or produced by non-thermal processing and bioprocessing: organic acids, antioxidants, aromas, sugars, fibres, proteins etc. These are the emerging technologies to increase the conversion efficiency, to decrease generation of toxic compounds, and to reduce microbial load of substrates. The selected cases of cascade and integrated processes were discussed, with examples focused on substrates, roles of non-thermal processing and bioprocessing in lactic acid production.
PB  - Springer Nature Switzerland AG
T2  - Nonthermal Processing in Agri-Food-Bio Sciences. Food Engineering Series.
T1  - Emerging Non-thermal Processing of Food Waste and by-Products for Sustainable Food Systems - Selected Cases
EP  - 709
SP  - 687
DO  - 10.1007/978-3-030-92415-7_24
ER  - 

@inbook{
author = "Đukić-Vuković, Aleksandra P. and Mladenović, Dragana D. and Pejin, Jelena D. and Mojović, Ljiljana V.",
year = "2022",
abstract = "Food waste and by-products are generated in all stages of food supply chain and it is estimated that third of all produced food is wasted. The goal is to minimise avoidable waste, to recover resources in sustainable way and to recycle them back into the food chain. A bibliometric mapping is used to analyse state of the art in non-thermal processing of food waste and by-products. Maximizing product yield, lowering energy consumption, economical, and environmental aspects are essential criteria for designing a new sustainable biorefinery process on food wastes and by-products. Many compounds important for food industry could be recovered or produced by non-thermal processing and bioprocessing: organic acids, antioxidants, aromas, sugars, fibres, proteins etc. These are the emerging technologies to increase the conversion efficiency, to decrease generation of toxic compounds, and to reduce microbial load of substrates. The selected cases of cascade and integrated processes were discussed, with examples focused on substrates, roles of non-thermal processing and bioprocessing in lactic acid production.",
publisher = "Springer Nature Switzerland AG",
journal = "Nonthermal Processing in Agri-Food-Bio Sciences. Food Engineering Series.",
booktitle = "Emerging Non-thermal Processing of Food Waste and by-Products for Sustainable Food Systems - Selected Cases",
pages = "709-687",
doi = "10.1007/978-3-030-92415-7_24"
}

Đukić-Vuković, A. P., Mladenović, D. D., Pejin, J. D.,& Mojović, L. V.. (2022). Emerging Non-thermal Processing of Food Waste and by-Products for Sustainable Food Systems - Selected Cases. in Nonthermal Processing in Agri-Food-Bio Sciences. Food Engineering Series.
Springer Nature Switzerland AG., 687-709.
https://doi.org/10.1007/978-3-030-92415-7_24

Đukić-Vuković AP, Mladenović DD, Pejin JD, Mojović LV. Emerging Non-thermal Processing of Food Waste and by-Products for Sustainable Food Systems - Selected Cases. in Nonthermal Processing in Agri-Food-Bio Sciences. Food Engineering Series.. 2022;:687-709.
doi:10.1007/978-3-030-92415-7_24 .

Đukić-Vuković, Aleksandra P., Mladenović, Dragana D., Pejin, Jelena D., Mojović, Ljiljana V., "Emerging Non-thermal Processing of Food Waste and by-Products for Sustainable Food Systems - Selected Cases" in Nonthermal Processing in Agri-Food-Bio Sciences. Food Engineering Series. (2022):687-709,
https://doi.org/10.1007/978-3-030-92415-7_24 . .

TY  - CONF
AU  - Đukić-Vuković, Aleksandra
AU  - Bogdanović, Mihajlo
AU  - Grbić, Jovana
AU  - Mladenović, Dragana
AU  - Mojović, Ljiljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6218
AB  - Common nettle (Urtica dioica) is widely spread medicinal plant growing in Europe. It is used as food,
herbal tea or extract in dietary supplements. Significant amount of common nettle herb residues
remains during standard herb processing in herbal tea production – due to grounding and fragility of
dried common nettle’s leaf. These residues are currently underexploited and are considered waste, but
could be used for extraction. Dried or liquid extracts of pharmacopoeial-level quality common nettle
are used in iron supplementation, in treatments of rheumatoid diseases, hyperthensia or hyperglycemic
disorders. Green solvents, water and ethanol are preferable for sustainable extractions and food and
health related applications. Additionally, these solvents favour extraction of polar compounds like
glycosides which could be metabolized by microorganisms. Water extracts do not harm
microorganisms and are used as such in fermentation while ethanol have to be removed before
fermentations.
In our work, we studied water extraction of common nettle herb and its residues and tested these
extracts as substrates for the growth of Ligilactobacillus salivarius and Lacticaseibacillus rhamnosus,
bacteria with probiotic potential. Antioxidant activity (DPPH and ABTS methods) and growth of L.
salivarius and L. rhamnosus were common nettle’s extracts obtained under different conditions. The
extracts obtained from residues showed comparable or even superior antioxidant activity to extracts
obtained from standard herb. Common nettle extracts without carbon or nitrogen supplementation were
poor substrates for the growth of L. salivarius and L. rhamnosus, while supplementation with broth in
low percentages resulted in growth stimulation above control. Findings suggest that common nettle
herb residues could be valorised in food chain avoiding landfill disposal and wasting of this valuable
resource.
AB  - Kopriva (Urtica dioica) je široko rasprostranjena biljka koja raste širom Evrope. Koristi se za ishranu,
kao čaj ili u formi ekstrakta u dijetetskim suplementima. Tokom prerade herbe koprive i pakovanja za
primenu u formi čajeva nastaje značajna količina otpada – izdrobljene herbe koprive. Ovaj otpad se za
sada ne koristi i smatra se otpadom, ali bi se mogao koristiti za ekstrakcije. Suvi i tečni ekstrakti
koprive farmakopejskog kvaliteta se koriste za tretmanu anemija, reumatoidnih bolesti, hipertenzije i
hiperglikemijskih poremećaja. Zeleni rastvarači, voda i etanol su najpogodniji za dobijanje ekstrakata
na održiv način za prehrambene i farmaceutske primene. Takođe, ovi rastvarači pogoduju ekstrakciji
polarnih jedinjenja kao što su glikozidi koje mogu da fermentišu mikroorganizmi. Vodeni ekstrakti se
mogu koristiti bez tretmana, jer ne utiču negativno na rast mikroorganizama, dok je iz etanolnih
ekstrakata neophodno ukloniti etanol pre fermentacije.
U radu smo proučavali vodenu ekstrakciju herbe koprive i otpada dobijenog preradom herbe. Testirali
smo ekstrakte kao supstrate za rast Ligilactobacillus salivarius i Lacticaseibacillus rhamnosus,
bakterija sa probiotskim potencijalom i antioksidantnu aktivnost ekstrakata (DPPH i ABTS metode).
Antioksidantna aktivnost i rast L. salivarius i L. rhamnosus su ispitivani u ektraktima koprive
dobijenim pod različitim uslovima. Ekstrakti dobijeni iz otpadna herbe koprive su pokazali sličnu ili
čak bolju antioksidativnu aktivnost u odnosu na ekstrakte standardno korišćene herbe. Ekstrakti
koprive bez dopune izvorima ugljenika ili azota su bili loš supstrat za rast L. salivarius i L. rhamnosus,
dok su ekstrakti obogaćeni bujonom u niskom procentu pokazali rast bolji od kontrolnog uzorka.
Rezultati rada ukazuju na potencijal otpada iz prerade koprive za primenu u prehrambenoj industriji
čime bi se izbeglo odlaganje na deponije i bacanje ovog vrednog resursa.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
C3  - 34th Scientific-Professional conference with international participation Process technology and energy in Agriculture – PTEP 2022
T1  - Common nettle as substrate for the growth of Lacticaseibacillus sp. and Ligilactobacillus sp.
T1  - Kopriva kao supstrat za rast Lacticaseibacillus sp. i Ligilactobacillus sp.
EP  - 18
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6218
ER  - 

@conference{
author = "Đukić-Vuković, Aleksandra and Bogdanović, Mihajlo and Grbić, Jovana and Mladenović, Dragana and Mojović, Ljiljana",
year = "2022",
abstract = "Common nettle (Urtica dioica) is widely spread medicinal plant growing in Europe. It is used as food,
herbal tea or extract in dietary supplements. Significant amount of common nettle herb residues
remains during standard herb processing in herbal tea production – due to grounding and fragility of
dried common nettle’s leaf. These residues are currently underexploited and are considered waste, but
could be used for extraction. Dried or liquid extracts of pharmacopoeial-level quality common nettle
are used in iron supplementation, in treatments of rheumatoid diseases, hyperthensia or hyperglycemic
disorders. Green solvents, water and ethanol are preferable for sustainable extractions and food and
health related applications. Additionally, these solvents favour extraction of polar compounds like
glycosides which could be metabolized by microorganisms. Water extracts do not harm
microorganisms and are used as such in fermentation while ethanol have to be removed before
fermentations.
In our work, we studied water extraction of common nettle herb and its residues and tested these
extracts as substrates for the growth of Ligilactobacillus salivarius and Lacticaseibacillus rhamnosus,
bacteria with probiotic potential. Antioxidant activity (DPPH and ABTS methods) and growth of L.
salivarius and L. rhamnosus were common nettle’s extracts obtained under different conditions. The
extracts obtained from residues showed comparable or even superior antioxidant activity to extracts
obtained from standard herb. Common nettle extracts without carbon or nitrogen supplementation were
poor substrates for the growth of L. salivarius and L. rhamnosus, while supplementation with broth in
low percentages resulted in growth stimulation above control. Findings suggest that common nettle
herb residues could be valorised in food chain avoiding landfill disposal and wasting of this valuable
resource., Kopriva (Urtica dioica) je široko rasprostranjena biljka koja raste širom Evrope. Koristi se za ishranu,
kao čaj ili u formi ekstrakta u dijetetskim suplementima. Tokom prerade herbe koprive i pakovanja za
primenu u formi čajeva nastaje značajna količina otpada – izdrobljene herbe koprive. Ovaj otpad se za
sada ne koristi i smatra se otpadom, ali bi se mogao koristiti za ekstrakcije. Suvi i tečni ekstrakti
koprive farmakopejskog kvaliteta se koriste za tretmanu anemija, reumatoidnih bolesti, hipertenzije i
hiperglikemijskih poremećaja. Zeleni rastvarači, voda i etanol su najpogodniji za dobijanje ekstrakata
na održiv način za prehrambene i farmaceutske primene. Takođe, ovi rastvarači pogoduju ekstrakciji
polarnih jedinjenja kao što su glikozidi koje mogu da fermentišu mikroorganizmi. Vodeni ekstrakti se
mogu koristiti bez tretmana, jer ne utiču negativno na rast mikroorganizama, dok je iz etanolnih
ekstrakata neophodno ukloniti etanol pre fermentacije.
U radu smo proučavali vodenu ekstrakciju herbe koprive i otpada dobijenog preradom herbe. Testirali
smo ekstrakte kao supstrate za rast Ligilactobacillus salivarius i Lacticaseibacillus rhamnosus,
bakterija sa probiotskim potencijalom i antioksidantnu aktivnost ekstrakata (DPPH i ABTS metode).
Antioksidantna aktivnost i rast L. salivarius i L. rhamnosus su ispitivani u ektraktima koprive
dobijenim pod različitim uslovima. Ekstrakti dobijeni iz otpadna herbe koprive su pokazali sličnu ili
čak bolju antioksidativnu aktivnost u odnosu na ekstrakte standardno korišćene herbe. Ekstrakti
koprive bez dopune izvorima ugljenika ili azota su bili loš supstrat za rast L. salivarius i L. rhamnosus,
dok su ekstrakti obogaćeni bujonom u niskom procentu pokazali rast bolji od kontrolnog uzorka.
Rezultati rada ukazuju na potencijal otpada iz prerade koprive za primenu u prehrambenoj industriji
čime bi se izbeglo odlaganje na deponije i bacanje ovog vrednog resursa.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "34th Scientific-Professional conference with international participation Process technology and energy in Agriculture – PTEP 2022",
title = "Common nettle as substrate for the growth of Lacticaseibacillus sp. and Ligilactobacillus sp., Kopriva kao supstrat za rast Lacticaseibacillus sp. i Ligilactobacillus sp.",
pages = "18-17",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6218"
}

Đukić-Vuković, A., Bogdanović, M., Grbić, J., Mladenović, D.,& Mojović, L.. (2022). Common nettle as substrate for the growth of Lacticaseibacillus sp. and Ligilactobacillus sp.. in 34th Scientific-Professional conference with international participation Process technology and energy in Agriculture – PTEP 2022
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi., 17-18.
https://hdl.handle.net/21.15107/rcub_technorep_6218

Đukić-Vuković A, Bogdanović M, Grbić J, Mladenović D, Mojović L. Common nettle as substrate for the growth of Lacticaseibacillus sp. and Ligilactobacillus sp.. in 34th Scientific-Professional conference with international participation Process technology and energy in Agriculture – PTEP 2022. 2022;:17-18.
https://hdl.handle.net/21.15107/rcub_technorep_6218 .

Đukić-Vuković, Aleksandra, Bogdanović, Mihajlo, Grbić, Jovana, Mladenović, Dragana, Mojović, Ljiljana, "Common nettle as substrate for the growth of Lacticaseibacillus sp. and Ligilactobacillus sp." in 34th Scientific-Professional conference with international participation Process technology and energy in Agriculture – PTEP 2022 (2022):17-18,
https://hdl.handle.net/21.15107/rcub_technorep_6218 .

TY  - CONF
AU  - Đukić-Vuković, Aleksandra
AU  - Grbić, Jovana
AU  - Mladenović, Dragana
AU  - Lazović, Saša
AU  - Mojović, Ljiljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6219
AB  - Introduction
Agricultural residues are abundant and cheap sources of lignocellulose widely available but with varying
composition which strongly affects valorization routes. Valorization of agricultural biomass into bio-based
chemicals is usually oriented towards treatments to release fermentable sugars from complex and strongly bonded
molecules of cellulose, hemicellulose and lignin within lignocellulose. Treatments of lignocellulose were mostly
studied to make it accessible to cellulases for hydrolisis of carbohydrates which could be then fermented into
organic acid by lactic acid bacteria or bioetahnol by Saccharomyces sp., for example. In these processes
valorization of lignin fraction was often neglected. However, lignin is among the most abundant aromatic polymers
on planet with estimated amounts of over 300 billion tons and with annual increments estimated by around 20
billion tons (Smolarski, 2012). Lignin could be a raw material for numerous currently petrol-based chemicals like
additives, thermoplastics and compounds used in pharmaceutical industry (Ponnusamy et al., 2019).
Material and methods
We studied the effects of advanced oxidation techniques, namely Fenton process, non-thermal plasma
treatment and combination of these treatments on degradation of corn stalks as agroindustry based lignocellulosic
substrate. Chemical composition was studied using FTIR, bioaccessibility and acetyl bromide soluble lignin
content were determined by spectrophotometric methods while morphology of samples was examined by SEM.
Different concentrations of Fe2+/Fe3+ and H2O2 were added to the grounded biomass of dried corn stalks and
subjected to the plasma treatment.
Results and Discussion
The results showed that Fe/H2O2 ratio strongly affects degradation outcomes, where 1:5 Fe/H2O2 affects
more carbohydrate fraction of substrate, while higher ratios are needed to affect significantly lignin fraction. The
effect is potentiated with additional plasma treatment. These results are in agreement with recently reported study
of Zhou et al (2020). Iodine sorption assey did not reveal significant changes in the bioaccesibility but FTIR
spectra showed changes in the number of H-bonds in treated substrate. Increase in the relative content of lignin in
plasma treated samples was probably a result of more prominent degradation of carbohydrate fraction, as
confirmed by an increase in glucose content in liquid fraction of treatment media.
Conclusion
Shorter non-thermal plasma treatments (10 min) do not affect significantly lignin fraction of corn stalks per se,
while addition of iron causes more significant changes in treated lignocellulose substrate under the milder
conditions. Obtained liquid fraction could be potentially valorized in fermentations after removal of excess iron,
while remaining lignin fraction could be modified or degraded by other means in biorefineries.
C3  - 9th International Conference on Sustainable Solid Waste Management
T1  - Advanced oxidation processes in treatment of agricultural biomass residues
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6219
ER  - 

@conference{
author = "Đukić-Vuković, Aleksandra and Grbić, Jovana and Mladenović, Dragana and Lazović, Saša and Mojović, Ljiljana",
year = "2022",
abstract = "Introduction
Agricultural residues are abundant and cheap sources of lignocellulose widely available but with varying
composition which strongly affects valorization routes. Valorization of agricultural biomass into bio-based
chemicals is usually oriented towards treatments to release fermentable sugars from complex and strongly bonded
molecules of cellulose, hemicellulose and lignin within lignocellulose. Treatments of lignocellulose were mostly
studied to make it accessible to cellulases for hydrolisis of carbohydrates which could be then fermented into
organic acid by lactic acid bacteria or bioetahnol by Saccharomyces sp., for example. In these processes
valorization of lignin fraction was often neglected. However, lignin is among the most abundant aromatic polymers
on planet with estimated amounts of over 300 billion tons and with annual increments estimated by around 20
billion tons (Smolarski, 2012). Lignin could be a raw material for numerous currently petrol-based chemicals like
additives, thermoplastics and compounds used in pharmaceutical industry (Ponnusamy et al., 2019).
Material and methods
We studied the effects of advanced oxidation techniques, namely Fenton process, non-thermal plasma
treatment and combination of these treatments on degradation of corn stalks as agroindustry based lignocellulosic
substrate. Chemical composition was studied using FTIR, bioaccessibility and acetyl bromide soluble lignin
content were determined by spectrophotometric methods while morphology of samples was examined by SEM.
Different concentrations of Fe2+/Fe3+ and H2O2 were added to the grounded biomass of dried corn stalks and
subjected to the plasma treatment.
Results and Discussion
The results showed that Fe/H2O2 ratio strongly affects degradation outcomes, where 1:5 Fe/H2O2 affects
more carbohydrate fraction of substrate, while higher ratios are needed to affect significantly lignin fraction. The
effect is potentiated with additional plasma treatment. These results are in agreement with recently reported study
of Zhou et al (2020). Iodine sorption assey did not reveal significant changes in the bioaccesibility but FTIR
spectra showed changes in the number of H-bonds in treated substrate. Increase in the relative content of lignin in
plasma treated samples was probably a result of more prominent degradation of carbohydrate fraction, as
confirmed by an increase in glucose content in liquid fraction of treatment media.
Conclusion
Shorter non-thermal plasma treatments (10 min) do not affect significantly lignin fraction of corn stalks per se,
while addition of iron causes more significant changes in treated lignocellulose substrate under the milder
conditions. Obtained liquid fraction could be potentially valorized in fermentations after removal of excess iron,
while remaining lignin fraction could be modified or degraded by other means in biorefineries.",
journal = "9th International Conference on Sustainable Solid Waste Management",
title = "Advanced oxidation processes in treatment of agricultural biomass residues",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6219"
}

Đukić-Vuković, A., Grbić, J., Mladenović, D., Lazović, S.,& Mojović, L.. (2022). Advanced oxidation processes in treatment of agricultural biomass residues. in 9th International Conference on Sustainable Solid Waste Management.
https://hdl.handle.net/21.15107/rcub_technorep_6219

Đukić-Vuković A, Grbić J, Mladenović D, Lazović S, Mojović L. Advanced oxidation processes in treatment of agricultural biomass residues. in 9th International Conference on Sustainable Solid Waste Management. 2022;.
https://hdl.handle.net/21.15107/rcub_technorep_6219 .

Đukić-Vuković, Aleksandra, Grbić, Jovana, Mladenović, Dragana, Lazović, Saša, Mojović, Ljiljana, "Advanced oxidation processes in treatment of agricultural biomass residues" in 9th International Conference on Sustainable Solid Waste Management (2022),
https://hdl.handle.net/21.15107/rcub_technorep_6219 .

TY  - CONF
AU  - Mladenović, Dragana
AU  - Grbić, Jovana
AU  - Đukić-Vuković, Aleksandra
AU  - Mojović, Ljiljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6282
PB  - National Technical University of Athens
C3  - 9th International Conference on Sustainable Solid Waste Management
T1  - Improvement of enzymatic saccharification of corn cob by microwave-assisted peroxide treatment
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6282
ER  - 

@conference{
author = "Mladenović, Dragana and Grbić, Jovana and Đukić-Vuković, Aleksandra and Mojović, Ljiljana",
year = "2022",
publisher = "National Technical University of Athens",
journal = "9th International Conference on Sustainable Solid Waste Management",
title = "Improvement of enzymatic saccharification of corn cob by microwave-assisted peroxide treatment",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6282"
}

Mladenović, D., Grbić, J., Đukić-Vuković, A.,& Mojović, L.. (2022). Improvement of enzymatic saccharification of corn cob by microwave-assisted peroxide treatment. in 9th International Conference on Sustainable Solid Waste Management
National Technical University of Athens..
https://hdl.handle.net/21.15107/rcub_technorep_6282

Mladenović D, Grbić J, Đukić-Vuković A, Mojović L. Improvement of enzymatic saccharification of corn cob by microwave-assisted peroxide treatment. in 9th International Conference on Sustainable Solid Waste Management. 2022;.
https://hdl.handle.net/21.15107/rcub_technorep_6282 .

Mladenović, Dragana, Grbić, Jovana, Đukić-Vuković, Aleksandra, Mojović, Ljiljana, "Improvement of enzymatic saccharification of corn cob by microwave-assisted peroxide treatment" in 9th International Conference on Sustainable Solid Waste Management (2022),
https://hdl.handle.net/21.15107/rcub_technorep_6282 .

TY  - CONF
AU  - Mladenović, Dragana
AU  - Grbić, Jovana
AU  - Đukić-Vuković, Aleksandra
AU  - Pejin, Jelena
AU  - Mojović, Ljiljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6284
AB  - Najveći deo ćelijskog zida viših biljaka se sastoji od tri polimerna molekula, celuloze, hemiceluloze i lignina. Imajući u vidu zastupljenost ova tri polimera u prirodi, lignocelulozna biomasa se danas smatra važnom sirovinom za proizvodnju biogoriva i biohemikalija. U navedenim procesima prvi i najvažniji korak je narušavanje složene strukture lignocelulozne biomase i oslobađanje polisaharidnih frakcija, čime se biomasa priprema za hidrolizu do prostih šećera. Cilj ovog rada bio je ispitati na koji način oksidativni tretman primenom vodonik peroksida u alkalnim uslovima utiče na karakteristike ćelijskog zida kukuruzne biomase i da li nastale promene u sastavu i strukturi tretirane biomase dovode do efikasnije enzimske hidrolize.
Kukuruzna biomasa je tretirana alkalnim rastvorom vodonik peroksida u mikrotalasnoj pećnici u
trajanju od 60 s i pri snazi od 360 W. Nakon ispiranja i sušenja, uzorci su podvrgnuti enzimskoj
hidrolizi pomoću enzima Cellic® CTec2, kao i odgovarajućim analitičkim metodama za određivanje
dostupnosti celuloze, kapaciteta adsorpcije enzima, indeksa kristaliničnosti i stepena delignifikacije. Efikasnost hidrolize tretirane i netretirane biomase je utvrđena merenjem koncentracije redukujućih šećera u dobijenim hidrolizatima. Dostupnost celuloze je ispitivana na osnovu adsorpcije boje Kongo crveno i izračunavanjem površine celuloze fitovanjem eksperimentalno dobijenih podataka sa jednačinom Lengmirove adsorpcione izoterme. Indeks kristaliničnosti je određen na osnovu vrednosti jodnog broja, a stepen delignifikacije acetil-bromid metodom. Takođe, ispitivanjem adsorpcije enzima Cellic® CTec2 i na osnovu konstanti Lengmirove adsorpcione izoterme utvrđen je maksimalni kapacitet adsorpcije enzima.
Dobijeni rezultati su pokazali da vodonik peroksid pri ispitivanim uslovima prvenstveno reaguje sa
ligninom. Udeo lignina u tretiranoj biomasi je bio za 70% manji u odnosu na netretirani uzorak. Usled smanjenja sadržaja lignina površina celuloze tretirane biomase (186,97 m2/g) je bila značajno veća u odnosu na netretiranu (57,36 m2/g), što znači da se ispitivanim tretmanom povećava površina celuloze koja je dostupna za vezivanje celulolitičkih enzima tokom hidrolize. Takođe, tretman je doveo do povećanja indeksa kristaliničnosti za 17,5%. Ispitivanjem adsorpcije enzima pokazano je da tretirana biomasa ima veći maksimalni kapacitet adsorpcije enzima u odnosu na netretirani uzorak. Konačno, hidrolizom tretirane biomase je postignuta značajno veća koncentracija redukujućih šećera (33,04 g/L) u odnosu na netretiranu biomasu (4,39 g/L). Rezultati ovog rada doprinose boljem razumevanju efekata ispitivanog tretmana i razvoju efikasnijeg procesa frakcionisanja biomase, a time i bolje hidrolize dobijenih frakcija do prostih šećera, koji se dalje mogu koristiti za fermentacionu proizvodnju biogoriva i/ili biohemikalija.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
C3  - XXXIV Nacionalna konferencija sa međunarodnim učešćem „Procesna tehnika i energetika u poljoprivredi - PTEP2022“
T1  - Karakteristike ćelijskog zida kukuruzne biomase nakon oksidativnog tretmana
SP  - 55
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6284
ER  - 

@conference{
author = "Mladenović, Dragana and Grbić, Jovana and Đukić-Vuković, Aleksandra and Pejin, Jelena and Mojović, Ljiljana",
year = "2022",
abstract = "Najveći deo ćelijskog zida viših biljaka se sastoji od tri polimerna molekula, celuloze, hemiceluloze i lignina. Imajući u vidu zastupljenost ova tri polimera u prirodi, lignocelulozna biomasa se danas smatra važnom sirovinom za proizvodnju biogoriva i biohemikalija. U navedenim procesima prvi i najvažniji korak je narušavanje složene strukture lignocelulozne biomase i oslobađanje polisaharidnih frakcija, čime se biomasa priprema za hidrolizu do prostih šećera. Cilj ovog rada bio je ispitati na koji način oksidativni tretman primenom vodonik peroksida u alkalnim uslovima utiče na karakteristike ćelijskog zida kukuruzne biomase i da li nastale promene u sastavu i strukturi tretirane biomase dovode do efikasnije enzimske hidrolize.
Kukuruzna biomasa je tretirana alkalnim rastvorom vodonik peroksida u mikrotalasnoj pećnici u
trajanju od 60 s i pri snazi od 360 W. Nakon ispiranja i sušenja, uzorci su podvrgnuti enzimskoj
hidrolizi pomoću enzima Cellic® CTec2, kao i odgovarajućim analitičkim metodama za određivanje
dostupnosti celuloze, kapaciteta adsorpcije enzima, indeksa kristaliničnosti i stepena delignifikacije. Efikasnost hidrolize tretirane i netretirane biomase je utvrđena merenjem koncentracije redukujućih šećera u dobijenim hidrolizatima. Dostupnost celuloze je ispitivana na osnovu adsorpcije boje Kongo crveno i izračunavanjem površine celuloze fitovanjem eksperimentalno dobijenih podataka sa jednačinom Lengmirove adsorpcione izoterme. Indeks kristaliničnosti je određen na osnovu vrednosti jodnog broja, a stepen delignifikacije acetil-bromid metodom. Takođe, ispitivanjem adsorpcije enzima Cellic® CTec2 i na osnovu konstanti Lengmirove adsorpcione izoterme utvrđen je maksimalni kapacitet adsorpcije enzima.
Dobijeni rezultati su pokazali da vodonik peroksid pri ispitivanim uslovima prvenstveno reaguje sa
ligninom. Udeo lignina u tretiranoj biomasi je bio za 70% manji u odnosu na netretirani uzorak. Usled smanjenja sadržaja lignina površina celuloze tretirane biomase (186,97 m2/g) je bila značajno veća u odnosu na netretiranu (57,36 m2/g), što znači da se ispitivanim tretmanom povećava površina celuloze koja je dostupna za vezivanje celulolitičkih enzima tokom hidrolize. Takođe, tretman je doveo do povećanja indeksa kristaliničnosti za 17,5%. Ispitivanjem adsorpcije enzima pokazano je da tretirana biomasa ima veći maksimalni kapacitet adsorpcije enzima u odnosu na netretirani uzorak. Konačno, hidrolizom tretirane biomase je postignuta značajno veća koncentracija redukujućih šećera (33,04 g/L) u odnosu na netretiranu biomasu (4,39 g/L). Rezultati ovog rada doprinose boljem razumevanju efekata ispitivanog tretmana i razvoju efikasnijeg procesa frakcionisanja biomase, a time i bolje hidrolize dobijenih frakcija do prostih šećera, koji se dalje mogu koristiti za fermentacionu proizvodnju biogoriva i/ili biohemikalija.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "XXXIV Nacionalna konferencija sa međunarodnim učešćem „Procesna tehnika i energetika u poljoprivredi - PTEP2022“",
title = "Karakteristike ćelijskog zida kukuruzne biomase nakon oksidativnog tretmana",
pages = "55",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6284"
}

Mladenović, D., Grbić, J., Đukić-Vuković, A., Pejin, J.,& Mojović, L.. (2022). Karakteristike ćelijskog zida kukuruzne biomase nakon oksidativnog tretmana. in XXXIV Nacionalna konferencija sa međunarodnim učešćem „Procesna tehnika i energetika u poljoprivredi - PTEP2022“
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 55.
https://hdl.handle.net/21.15107/rcub_technorep_6284

Mladenović D, Grbić J, Đukić-Vuković A, Pejin J, Mojović L. Karakteristike ćelijskog zida kukuruzne biomase nakon oksidativnog tretmana. in XXXIV Nacionalna konferencija sa međunarodnim učešćem „Procesna tehnika i energetika u poljoprivredi - PTEP2022“. 2022;:55.
https://hdl.handle.net/21.15107/rcub_technorep_6284 .

Mladenović, Dragana, Grbić, Jovana, Đukić-Vuković, Aleksandra, Pejin, Jelena, Mojović, Ljiljana, "Karakteristike ćelijskog zida kukuruzne biomase nakon oksidativnog tretmana" in XXXIV Nacionalna konferencija sa međunarodnim učešćem „Procesna tehnika i energetika u poljoprivredi - PTEP2022“ (2022):55,
https://hdl.handle.net/21.15107/rcub_technorep_6284 .

TY  - JOUR
AU  - Jović, Jelena
AU  - Hao, Jian
AU  - Kocić-Tanackov, Sunčica
AU  - Mojović, Ljiljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/12
AB  - Fungal treatment of lignocellulose is an ecologically and economically acceptable method. However, it needs improvement to increase the hydrolysis rate. A novel combination of multiple response optimization of ligninolytic activity ofTrametes hirsutaF13 and supplementation of the lignocellulosic substrate (beechwood sawdust) with sugar beet molasses stillage was employed to improve and manage the desired type of fungal ligninolytic activity leading to a significant enhancement of biomass saccharification. Obtained optimal cultivation conditions (molasses stillage concentration, 13%; substrate moisture, 63%; and temperature, 25 degrees C) provided the desired combination of laccase and manganese-dependent peroxidase activity, and maintained the activities longer and at higher values (51.5 +/- 3.5 U/L and 91 +/- 4.24 U/L, respectively) than the conditions without molasses stillage (21.5 +/- 2.12 U/L and 31.5 +/- 4.9 U/L, respectively). Enzyme hydrolysis of the substrate treated for 7 days in the presence of molasses stillage released 20.54 +/- 0.80 mg/mL fermentable sugars, and 63.58 +/- 1.47 mg/mL from 18-days treated substrate, while sugar concentrations released from the substrates pretreated without the supplementation were 16.84 +/- 0.30 mg/mL and 53.63 +/- 2.16 mg/mL, respectively. The proposed new approach of the lignocellulose supplementation with sugar beet molasses stillage and manipulation and improvement of fungal ligninolytic activity proved to be a promising solution for the enhancement of lignocellulose bioconversion.
PB  - Springer Heidelberg, Heidelberg
T2  - Biomass Conversion and Biorefinery
T1  - Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation
EP  - 2765
IS  - 7
SP  - 2749
VL  - 12
DO  - 10.1007/s13399-020-00929-1
ER  - 

@article{
author = "Jović, Jelena and Hao, Jian and Kocić-Tanackov, Sunčica and Mojović, Ljiljana",
year = "2022",
abstract = "Fungal treatment of lignocellulose is an ecologically and economically acceptable method. However, it needs improvement to increase the hydrolysis rate. A novel combination of multiple response optimization of ligninolytic activity ofTrametes hirsutaF13 and supplementation of the lignocellulosic substrate (beechwood sawdust) with sugar beet molasses stillage was employed to improve and manage the desired type of fungal ligninolytic activity leading to a significant enhancement of biomass saccharification. Obtained optimal cultivation conditions (molasses stillage concentration, 13%; substrate moisture, 63%; and temperature, 25 degrees C) provided the desired combination of laccase and manganese-dependent peroxidase activity, and maintained the activities longer and at higher values (51.5 +/- 3.5 U/L and 91 +/- 4.24 U/L, respectively) than the conditions without molasses stillage (21.5 +/- 2.12 U/L and 31.5 +/- 4.9 U/L, respectively). Enzyme hydrolysis of the substrate treated for 7 days in the presence of molasses stillage released 20.54 +/- 0.80 mg/mL fermentable sugars, and 63.58 +/- 1.47 mg/mL from 18-days treated substrate, while sugar concentrations released from the substrates pretreated without the supplementation were 16.84 +/- 0.30 mg/mL and 53.63 +/- 2.16 mg/mL, respectively. The proposed new approach of the lignocellulose supplementation with sugar beet molasses stillage and manipulation and improvement of fungal ligninolytic activity proved to be a promising solution for the enhancement of lignocellulose bioconversion.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Biomass Conversion and Biorefinery",
title = "Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation",
pages = "2765-2749",
number = "7",
volume = "12",
doi = "10.1007/s13399-020-00929-1"
}

Jović, J., Hao, J., Kocić-Tanackov, S.,& Mojović, L.. (2022). Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation. in Biomass Conversion and Biorefinery
Springer Heidelberg, Heidelberg., 12(7), 2749-2765.
https://doi.org/10.1007/s13399-020-00929-1

Jović J, Hao J, Kocić-Tanackov S, Mojović L. Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation. in Biomass Conversion and Biorefinery. 2022;12(7):2749-2765.
doi:10.1007/s13399-020-00929-1 .

Jović, Jelena, Hao, Jian, Kocić-Tanackov, Sunčica, Mojović, Ljiljana, "Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation" in Biomass Conversion and Biorefinery, 12, no. 7 (2022):2749-2765,
https://doi.org/10.1007/s13399-020-00929-1 . .

TY  - JOUR
AU  - Jovic, Jelena
AU  - Hao, Jian
AU  - Mojović, Ljiljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4975
AB  - This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses still age. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g(-1) substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g(-1) substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 degrees C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentration differed depending on the enzyme type (for laccase it was   lt 5 %, manganese peroxidase approximate to 12 %, cellulase approximate to 21 % and xylanase approximate to 16 %), which should be taken into consideration when optimizing the desired process..
T2  - Journal of the Serbian Chemical Society
T1  - Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage
DO  - 10.2298/JSC200601075J
ER  - 

@article{
author = "Jovic, Jelena and Hao, Jian and Mojović, Ljiljana",
year = "2022",
abstract = "This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses still age. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g(-1) substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g(-1) substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 degrees C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentration differed depending on the enzyme type (for laccase it was   lt 5 %, manganese peroxidase approximate to 12 %, cellulase approximate to 21 % and xylanase approximate to 16 %), which should be taken into consideration when optimizing the desired process..",
journal = "Journal of the Serbian Chemical Society",
title = "Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage",
doi = "10.2298/JSC200601075J"
}

Jovic, J., Hao, J.,& Mojović, L.. (2022). Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage. in Journal of the Serbian Chemical Society.
https://doi.org/10.2298/JSC200601075J

Jovic J, Hao J, Mojović L. Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage. in Journal of the Serbian Chemical Society. 2022;.
doi:10.2298/JSC200601075J .

Jovic, Jelena, Hao, Jian, Mojović, Ljiljana, "Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage" in Journal of the Serbian Chemical Society (2022),
https://doi.org/10.2298/JSC200601075J . .

TY  - JOUR
AU  - Wang, Qinghui
AU  - Gu, Jinjie
AU  - Shu, Lin
AU  - Jiang, Weiyan
AU  - Mojović, Ljiljana
AU  - Knežević-Jugović, Zorica
AU  - Shi, Jiping
AU  - Baganz, Frank
AU  - Lye, Gary J.
AU  - Xiang, Wensheng
AU  - Hao, Jian
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5109
AB  - Klebsiella pneumoniae is a 2,3-butanediol producing bacterium. Nevertheless, a design and construction of l-valine production strain was studied in this paper. The first step of 2,3-butanediol synthesis and branched-chain amino acid synthesis pathways share the same step of α-acetolactate synthesis from pyruvate. However, the two pathways are existing in parallel and do not interfere with each other in the wild-type strain. A knockout of budA blocked the 2,3-butanediol synthesis pathway and resulted in the l-valine production. The budA coded an α-acetolactate decarboxylase and catalyzed the acetoin formation from α-acetolactate. Furthermore, blocking the lactic acid synthesis by knocking out of ldhA, which is encoding a lactate dehydrogenase, improved the l-valine synthesis. 2-Ketoisovalerate is the precursor of l-valine, it is also an intermediate of the isobutanol synthesis pathway, while indole-3-pyruvate decarboxylase (ipdC) is responsible for isobutyraldehyde formation from 2-ketoisovalerate. Production of l-valine has been improved by knocking out of ipdC. On the other side, the ilvE, encoding a transaminase B, reversibly transfers one amino group from glutamate to α-ketoisovalerate. Overexpression of ilvE exhibited a distinct improvement of l-valine production. The brnQ encodes a branched-chain amino acid transporter, and l-valine production was further improved by disrupting brnQ. It is also revealed that weak acidic and aerobic conditions favor l-valine production. Based on these findings, l-valine production by metabolically engineered K. pneumonia was examined. In fed-batch fermentation, 22.4 g/L of l-valine was produced by the engineered K. pneumoniae ΔbudA-ΔldhA-ΔipdC-ΔbrnQ-ilvE after 55 h of cultivation, with a substrate conversion ratio of 0.27 mol/mol glucose.
PB  - Springer Science and Business Media B.V.
T2  - World Journal of Microbiology and Biotechnology
T1  - Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production
IS  - 5
SP  - 81
VL  - 38
DO  - 10.1007/s11274-022-03266-9
ER  - 

@article{
author = "Wang, Qinghui and Gu, Jinjie and Shu, Lin and Jiang, Weiyan and Mojović, Ljiljana and Knežević-Jugović, Zorica and Shi, Jiping and Baganz, Frank and Lye, Gary J. and Xiang, Wensheng and Hao, Jian",
year = "2022",
abstract = "Klebsiella pneumoniae is a 2,3-butanediol producing bacterium. Nevertheless, a design and construction of l-valine production strain was studied in this paper. The first step of 2,3-butanediol synthesis and branched-chain amino acid synthesis pathways share the same step of α-acetolactate synthesis from pyruvate. However, the two pathways are existing in parallel and do not interfere with each other in the wild-type strain. A knockout of budA blocked the 2,3-butanediol synthesis pathway and resulted in the l-valine production. The budA coded an α-acetolactate decarboxylase and catalyzed the acetoin formation from α-acetolactate. Furthermore, blocking the lactic acid synthesis by knocking out of ldhA, which is encoding a lactate dehydrogenase, improved the l-valine synthesis. 2-Ketoisovalerate is the precursor of l-valine, it is also an intermediate of the isobutanol synthesis pathway, while indole-3-pyruvate decarboxylase (ipdC) is responsible for isobutyraldehyde formation from 2-ketoisovalerate. Production of l-valine has been improved by knocking out of ipdC. On the other side, the ilvE, encoding a transaminase B, reversibly transfers one amino group from glutamate to α-ketoisovalerate. Overexpression of ilvE exhibited a distinct improvement of l-valine production. The brnQ encodes a branched-chain amino acid transporter, and l-valine production was further improved by disrupting brnQ. It is also revealed that weak acidic and aerobic conditions favor l-valine production. Based on these findings, l-valine production by metabolically engineered K. pneumonia was examined. In fed-batch fermentation, 22.4 g/L of l-valine was produced by the engineered K. pneumoniae ΔbudA-ΔldhA-ΔipdC-ΔbrnQ-ilvE after 55 h of cultivation, with a substrate conversion ratio of 0.27 mol/mol glucose.",
publisher = "Springer Science and Business Media B.V.",
journal = "World Journal of Microbiology and Biotechnology",
title = "Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production",
number = "5",
pages = "81",
volume = "38",
doi = "10.1007/s11274-022-03266-9"
}

Wang, Q., Gu, J., Shu, L., Jiang, W., Mojović, L., Knežević-Jugović, Z., Shi, J., Baganz, F., Lye, G. J., Xiang, W.,& Hao, J.. (2022). Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production. in World Journal of Microbiology and Biotechnology
Springer Science and Business Media B.V.., 38(5), 81.
https://doi.org/10.1007/s11274-022-03266-9

Wang Q, Gu J, Shu L, Jiang W, Mojović L, Knežević-Jugović Z, Shi J, Baganz F, Lye GJ, Xiang W, Hao J. Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production. in World Journal of Microbiology and Biotechnology. 2022;38(5):81.
doi:10.1007/s11274-022-03266-9 .

Wang, Qinghui, Gu, Jinjie, Shu, Lin, Jiang, Weiyan, Mojović, Ljiljana, Knežević-Jugović, Zorica, Shi, Jiping, Baganz, Frank, Lye, Gary J., Xiang, Wensheng, Hao, Jian, "Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production" in World Journal of Microbiology and Biotechnology, 38, no. 5 (2022):81,
https://doi.org/10.1007/s11274-022-03266-9 . .

TY  - JOUR
AU  - Jović, Jelena
AU  - Hao, Jian
AU  - Mojović, Ljiljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5182
AB  - This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses stillage. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g-1 substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g-1 substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 °C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentration differed depending on the enzyme type (for laccase it was <5 %, manganese peroxidase ≈12 %, cellulase ≈21 % and xylanase ≈16 %), which should be taken into consideration when optimizing the desired process.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage
EP  - 450
IS  - 4
SP  - 437
VL  - 87
DO  - 10.2298/JSC200601075J
ER  - 

@article{
author = "Jović, Jelena and Hao, Jian and Mojović, Ljiljana",
year = "2022",
abstract = "This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses stillage. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g-1 substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g-1 substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 °C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentration differed depending on the enzyme type (for laccase it was <5 %, manganese peroxidase ≈12 %, cellulase ≈21 % and xylanase ≈16 %), which should be taken into consideration when optimizing the desired process.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage",
pages = "450-437",
number = "4",
volume = "87",
doi = "10.2298/JSC200601075J"
}

Jović, J., Hao, J.,& Mojović, L.. (2022). Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 87(4), 437-450.
https://doi.org/10.2298/JSC200601075J

Jović J, Hao J, Mojović L. Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage. in Journal of the Serbian Chemical Society. 2022;87(4):437-450.
doi:10.2298/JSC200601075J .

Jović, Jelena, Hao, Jian, Mojović, Ljiljana, "Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage" in Journal of the Serbian Chemical Society, 87, no. 4 (2022):437-450,
https://doi.org/10.2298/JSC200601075J . .

TY  - JOUR
AU  - Gazikalović, Ivana
AU  - Mijalković, Jelena
AU  - Šekuljica, Nataša
AU  - Jakovetić Tanasković, Sonja
AU  - Đukić-Vuković, Aleksandra
AU  - Mojović, Ljiljana
AU  - Knežević-Jugović, Zorica
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4811
AB  - In this study, we assessed the effects of microwave irradiation of wheat gluten proteins as a pretreatment performed in a microwave reactor that could accurately control process parameters as a function of power and temperature, as well as comparing it with conventional heat treatment. The aim was to identify suitable combinations of partial enzymatic hydrolysis and microwave pretreatment parameters to produce gluten hydrolysates with reduced allergenicity and conserved techno-functional features for food application. FTIR analysis, and total and reactive SH group contents confirmed that the microwave-controlled heating can significantly change the secondary structure and conformation of gluten protein. The microwave treatment had the largest effect at 200 W and 100 degrees C, at which the content of gluten has been reduced by about 2.5-fold. The microwave pretreatment also accelerated the enzymatic hydrolysis of gluten, changing the kinetic profile. The apparent hydrolysis rate constants (k(2)) were 1.00, 3.68, 3.48, 4.64 and 4.17 min(-1) for untreated gluten, and those pretreated with microwave power of 200, 400, 600 and 800 W, respectively. Compared to the heat treatment, it appeared that microwave specific non-thermal effects had a significant influence on the gluten structure and allergenicity and, in combination with the enzymatic hydrolysis, ultimately yielded protein hydrolysates with enhanced antioxidant and functional properties.
T2  - Foods
T1  - Synergistic Effect of Enzyme Hydrolysis and Microwave Reactor Pretreatment as an Efficient Procedure for Gluten Content Reduction
IS  - 9
VL  - 10
DO  - 10.3390/foods10092214
ER  - 

@article{
author = "Gazikalović, Ivana and Mijalković, Jelena and Šekuljica, Nataša and Jakovetić Tanasković, Sonja and Đukić-Vuković, Aleksandra and Mojović, Ljiljana and Knežević-Jugović, Zorica",
year = "2021",
abstract = "In this study, we assessed the effects of microwave irradiation of wheat gluten proteins as a pretreatment performed in a microwave reactor that could accurately control process parameters as a function of power and temperature, as well as comparing it with conventional heat treatment. The aim was to identify suitable combinations of partial enzymatic hydrolysis and microwave pretreatment parameters to produce gluten hydrolysates with reduced allergenicity and conserved techno-functional features for food application. FTIR analysis, and total and reactive SH group contents confirmed that the microwave-controlled heating can significantly change the secondary structure and conformation of gluten protein. The microwave treatment had the largest effect at 200 W and 100 degrees C, at which the content of gluten has been reduced by about 2.5-fold. The microwave pretreatment also accelerated the enzymatic hydrolysis of gluten, changing the kinetic profile. The apparent hydrolysis rate constants (k(2)) were 1.00, 3.68, 3.48, 4.64 and 4.17 min(-1) for untreated gluten, and those pretreated with microwave power of 200, 400, 600 and 800 W, respectively. Compared to the heat treatment, it appeared that microwave specific non-thermal effects had a significant influence on the gluten structure and allergenicity and, in combination with the enzymatic hydrolysis, ultimately yielded protein hydrolysates with enhanced antioxidant and functional properties.",
journal = "Foods",
title = "Synergistic Effect of Enzyme Hydrolysis and Microwave Reactor Pretreatment as an Efficient Procedure for Gluten Content Reduction",
number = "9",
volume = "10",
doi = "10.3390/foods10092214"
}

Gazikalović, I., Mijalković, J., Šekuljica, N., Jakovetić Tanasković, S., Đukić-Vuković, A., Mojović, L.,& Knežević-Jugović, Z.. (2021). Synergistic Effect of Enzyme Hydrolysis and Microwave Reactor Pretreatment as an Efficient Procedure for Gluten Content Reduction. in Foods, 10(9).
https://doi.org/10.3390/foods10092214

Gazikalović I, Mijalković J, Šekuljica N, Jakovetić Tanasković S, Đukić-Vuković A, Mojović L, Knežević-Jugović Z. Synergistic Effect of Enzyme Hydrolysis and Microwave Reactor Pretreatment as an Efficient Procedure for Gluten Content Reduction. in Foods. 2021;10(9).
doi:10.3390/foods10092214 .

Gazikalović, Ivana, Mijalković, Jelena, Šekuljica, Nataša, Jakovetić Tanasković, Sonja, Đukić-Vuković, Aleksandra, Mojović, Ljiljana, Knežević-Jugović, Zorica, "Synergistic Effect of Enzyme Hydrolysis and Microwave Reactor Pretreatment as an Efficient Procedure for Gluten Content Reduction" in Foods, 10, no. 9 (2021),
https://doi.org/10.3390/foods10092214 . .

TY  - CONF
AU  - Đukić-Vuković, Aleksandra
AU  - Mladenović, Dragana
AU  - Lakićević, B.
AU  - Mojović, Ljiljana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5932
AB  - Lactic acid bacteria (LAB) have acted in food fermentations through the ages due to their safety and resilience to specific harsh conditions of high salinity or low pH present in food and gut where they live. Their interaction with human technological development started in food but goes beyond, as some LAB contribute to the health of humans and animals as probiotics. The stress tolerance of LAB also makes them excellent, robust industrial microorganisms for production of lactic acid and other chemicals. The lactic acid market has had a high growth rate in the last decade mainly due to expansion of poly-lactide production. Poly-lactides are biocompatible, thermostable and biodegradable polymers of lactic acid, suitable for use in food packaging or in medicine, as scaffolds, implants or delivery systems. The ability of LAB to grow on complex waste substrates but efficiently produce selected isomers of lactic acid has positioned them at the core of bio-based packaging production, and this field is expected to grow in the future. Therefore, LAB are important for food - for preservation, flavour and packaging, but also beyond food - as probiotics, paraprobiotics and postbiotics. Recent trends in these fields of LAB application are analysed in this work.
PB  - IOP Publishing Ltd
C3  - IOP Conference Series: Earth and Environmental Science
T1  - Lactic acid bacteria: From food preservation to active packaging
IS  - 1
SP  - 012025
VL  - 854
DO  - 10.1088/1755-1315/854/1/012025
ER  - 

@conference{
author = "Đukić-Vuković, Aleksandra and Mladenović, Dragana and Lakićević, B. and Mojović, Ljiljana",
year = "2021",
abstract = "Lactic acid bacteria (LAB) have acted in food fermentations through the ages due to their safety and resilience to specific harsh conditions of high salinity or low pH present in food and gut where they live. Their interaction with human technological development started in food but goes beyond, as some LAB contribute to the health of humans and animals as probiotics. The stress tolerance of LAB also makes them excellent, robust industrial microorganisms for production of lactic acid and other chemicals. The lactic acid market has had a high growth rate in the last decade mainly due to expansion of poly-lactide production. Poly-lactides are biocompatible, thermostable and biodegradable polymers of lactic acid, suitable for use in food packaging or in medicine, as scaffolds, implants or delivery systems. The ability of LAB to grow on complex waste substrates but efficiently produce selected isomers of lactic acid has positioned them at the core of bio-based packaging production, and this field is expected to grow in the future. Therefore, LAB are important for food - for preservation, flavour and packaging, but also beyond food - as probiotics, paraprobiotics and postbiotics. Recent trends in these fields of LAB application are analysed in this work.",
publisher = "IOP Publishing Ltd",
journal = "IOP Conference Series: Earth and Environmental Science",
title = "Lactic acid bacteria: From food preservation to active packaging",
number = "1",
pages = "012025",
volume = "854",
doi = "10.1088/1755-1315/854/1/012025"
}

Đukić-Vuković, A., Mladenović, D., Lakićević, B.,& Mojović, L.. (2021). Lactic acid bacteria: From food preservation to active packaging. in IOP Conference Series: Earth and Environmental Science
IOP Publishing Ltd., 854(1), 012025.
https://doi.org/10.1088/1755-1315/854/1/012025

Đukić-Vuković A, Mladenović D, Lakićević B, Mojović L. Lactic acid bacteria: From food preservation to active packaging. in IOP Conference Series: Earth and Environmental Science. 2021;854(1):012025.
doi:10.1088/1755-1315/854/1/012025 .

Đukić-Vuković, Aleksandra, Mladenović, Dragana, Lakićević, B., Mojović, Ljiljana, "Lactic acid bacteria: From food preservation to active packaging" in IOP Conference Series: Earth and Environmental Science, 854, no. 1 (2021):012025,
https://doi.org/10.1088/1755-1315/854/1/012025 . .

TY  - CONF
AU  - Đukić-Vuković, Aleksandra
AU  - Mladenović, Dragana
AU  - Grbić, Jovana
AU  - Lazović, Saša
AU  - Pejin, Jelena
AU  - Kocić-Tanackov, Sunčica
AU  - Mojović, Ljiljana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6228
AB  - Lignocellulosic substrates are abundant, and considered cheap sources of cellulose for biorefinery
processes. However, their use is limited because of complex and interlinked structures of cellulose
and lignin not easily accessible to enzymes for decomposition. Different chemical (acid, base, and
enzymatic hydrolysis) and physical pretreatments (e.g. steam explosion, high pressure, non-thermal
plasma) are used with limited success for degradation of lignin and better utilization of cellulose by
microorganisms during fermentation. Poor selectivity, low efficiency, and environmental hazards
are common problems in treatment of lignocellulose. Degradation of both cellulose and lignin, and
formation of compounds which inhibit microorganisms in subsequent fermentation often occurs
reducing the efficiency of bioprocessing.
The use of a low-power atmospheric pressure plasma source (plasma needle) was investigated for
treatment of corn stalks. Slices of corn stalks were subjected to non-thermal plasma treatment under
different conditions. After the treatment, they are dyed and studied using light microscopy.
AstraBlue® and safranin are used as dyes for staining of lignin in the absence and presence of
cellulose, respectively. Plasma can degrade both cellulose and lignin but at different rates and
treatment conditions can be tailored to achieve selectivity. This differential staining method is fast
and simple. It enables us to examine how selective plasma treatments are and has a potential for
screening of lignocellulose substrates
AB  - Lignocelulozni supstrati su široko rasprostranjeni i predstavljaju jeftine izvore celuloze za
biorafinerijske postupke. Pa ipak, njihova upotreba je ograničena zbog kompleksnog sastava i
izprepletanosti celuloznih i ligninskih komponenata što ih čini teško dostupnim za enzimsku
razgradnju. Različiti hemijski (kisela, bazna i enzimska hidroliza) i fizički pretretmani (npr. tretman
vodenom parom, visokim pritiskom, netermalnom plazmom) se koriste sa ograničenim uspehom za
degradaciju lignina i bolje iskorišćenje celuloze pomoću mikroorganizama u toku fermentacije.
Loša selektivnost, niska efikasnost i rizici vezani za uticaj na životnu sredinu su česti problemi
prilikom tretmana lignoceluloznih supstrata. U toku ovih tretmana, često dolazi do istovremene
razgradnje i celuloze i lignina i stvaranja inhibitornih jedinjenja koja negativno utiču na
mikroorganizame u toku fermentacije, utičući značajno na efikasnost celokupnog procesa.
U radu je ispitivana upotreba plazma izvora niske snage na atmosferskom pritisku (plazma igle) za
tretman stabljika kukuruza. Preseci stabljika kukuruza su bili izloženi netermalnoj plazmi pri
različitim uslovima. Nakon tretmana, preseci su obojeni i posmatrani svetlosnim mikroskopom.
AstraBlue® i safranin su korišćeni kao boje za bojenje lignina u odsustvu i prisustvu celuloze,
redom. Pokazano je da plazma može da razgradi i lignin i celulozu, ali u različitoj meri i da uslovi
tretmana mogu biti kontrolisani tako da utiču na selektivnost razgradnje. Ovaj metod
diferencijalnog bojenja je brz i jednostavan. On omogućava da se ispita selektivnost tretmana
plazmom i ima potencijal za primenu u brzom skriningu lignoceluloznih supstrata
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
C3  - 7th International conference Sustainable postharvest and food technologies - INOPTEP 2021
T1  - Non-thermal plasma treatment of corn stalks examined by light microscopy
T1  - Tretman preseka stabljika kukuruza netermalnom plazmom ispitivan svetlosnom mikroskopijom
EP  - 30
SP  - 29
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6228
ER  - 

@conference{
author = "Đukić-Vuković, Aleksandra and Mladenović, Dragana and Grbić, Jovana and Lazović, Saša and Pejin, Jelena and Kocić-Tanackov, Sunčica and Mojović, Ljiljana",
year = "2021",
abstract = "Lignocellulosic substrates are abundant, and considered cheap sources of cellulose for biorefinery
processes. However, their use is limited because of complex and interlinked structures of cellulose
and lignin not easily accessible to enzymes for decomposition. Different chemical (acid, base, and
enzymatic hydrolysis) and physical pretreatments (e.g. steam explosion, high pressure, non-thermal
plasma) are used with limited success for degradation of lignin and better utilization of cellulose by
microorganisms during fermentation. Poor selectivity, low efficiency, and environmental hazards
are common problems in treatment of lignocellulose. Degradation of both cellulose and lignin, and
formation of compounds which inhibit microorganisms in subsequent fermentation often occurs
reducing the efficiency of bioprocessing.
The use of a low-power atmospheric pressure plasma source (plasma needle) was investigated for
treatment of corn stalks. Slices of corn stalks were subjected to non-thermal plasma treatment under
different conditions. After the treatment, they are dyed and studied using light microscopy.
AstraBlue® and safranin are used as dyes for staining of lignin in the absence and presence of
cellulose, respectively. Plasma can degrade both cellulose and lignin but at different rates and
treatment conditions can be tailored to achieve selectivity. This differential staining method is fast
and simple. It enables us to examine how selective plasma treatments are and has a potential for
screening of lignocellulose substrates, Lignocelulozni supstrati su široko rasprostranjeni i predstavljaju jeftine izvore celuloze za
biorafinerijske postupke. Pa ipak, njihova upotreba je ograničena zbog kompleksnog sastava i
izprepletanosti celuloznih i ligninskih komponenata što ih čini teško dostupnim za enzimsku
razgradnju. Različiti hemijski (kisela, bazna i enzimska hidroliza) i fizički pretretmani (npr. tretman
vodenom parom, visokim pritiskom, netermalnom plazmom) se koriste sa ograničenim uspehom za
degradaciju lignina i bolje iskorišćenje celuloze pomoću mikroorganizama u toku fermentacije.
Loša selektivnost, niska efikasnost i rizici vezani za uticaj na životnu sredinu su česti problemi
prilikom tretmana lignoceluloznih supstrata. U toku ovih tretmana, često dolazi do istovremene
razgradnje i celuloze i lignina i stvaranja inhibitornih jedinjenja koja negativno utiču na
mikroorganizame u toku fermentacije, utičući značajno na efikasnost celokupnog procesa.
U radu je ispitivana upotreba plazma izvora niske snage na atmosferskom pritisku (plazma igle) za
tretman stabljika kukuruza. Preseci stabljika kukuruza su bili izloženi netermalnoj plazmi pri
različitim uslovima. Nakon tretmana, preseci su obojeni i posmatrani svetlosnim mikroskopom.
AstraBlue® i safranin su korišćeni kao boje za bojenje lignina u odsustvu i prisustvu celuloze,
redom. Pokazano je da plazma može da razgradi i lignin i celulozu, ali u različitoj meri i da uslovi
tretmana mogu biti kontrolisani tako da utiču na selektivnost razgradnje. Ovaj metod
diferencijalnog bojenja je brz i jednostavan. On omogućava da se ispita selektivnost tretmana
plazmom i ima potencijal za primenu u brzom skriningu lignoceluloznih supstrata",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "7th International conference Sustainable postharvest and food technologies - INOPTEP 2021",
title = "Non-thermal plasma treatment of corn stalks examined by light microscopy, Tretman preseka stabljika kukuruza netermalnom plazmom ispitivan svetlosnom mikroskopijom",
pages = "30-29",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6228"
}

Đukić-Vuković, A., Mladenović, D., Grbić, J., Lazović, S., Pejin, J., Kocić-Tanackov, S.,& Mojović, L.. (2021). Non-thermal plasma treatment of corn stalks examined by light microscopy. in 7th International conference Sustainable postharvest and food technologies - INOPTEP 2021
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi., 29-30.
https://hdl.handle.net/21.15107/rcub_technorep_6228

Đukić-Vuković A, Mladenović D, Grbić J, Lazović S, Pejin J, Kocić-Tanackov S, Mojović L. Non-thermal plasma treatment of corn stalks examined by light microscopy. in 7th International conference Sustainable postharvest and food technologies - INOPTEP 2021. 2021;:29-30.
https://hdl.handle.net/21.15107/rcub_technorep_6228 .

Đukić-Vuković, Aleksandra, Mladenović, Dragana, Grbić, Jovana, Lazović, Saša, Pejin, Jelena, Kocić-Tanackov, Sunčica, Mojović, Ljiljana, "Non-thermal plasma treatment of corn stalks examined by light microscopy" in 7th International conference Sustainable postharvest and food technologies - INOPTEP 2021 (2021):29-30,
https://hdl.handle.net/21.15107/rcub_technorep_6228 .