TY  - JOUR
AU  - Veljković, Milica
AU  - Banjanac, Katarina
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Simović, Milica
AU  - Bezbradica, Dejan
PY  - 2024-08
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7425
AB  - Maple syrup, a popular natural sweetener has a high content of sucrose, whose consumption is linked to different health issues such as obesity and diabetes. Hence, within this paper, the conversion of sucrose to prebiotics (fructo-oligosaccharides, FOS) was proposed as a promising approach to obtaining a healthier, value-added product. Enzymatic conversion was optimized with respect to key experimental factors, and thereafter derived immobilized preparation of fructosyltransferase (FTase) from Pectinex® Ultra SP-L (FTase-epoxy Purolite, 255 IU/g support) was successfully utilized to produce novel functional product in ten consecutive reaction cycles. The product, obtained under optimal conditions (60 ◦C, 7.65 IU/mL, 12 h), resulted in 56.0% FOS, 16.7% sucrose, and 27.3% monosaccharides of total carbohydrates, leading to a 1.6-fold reduction in caloric content. The obtained products` prebiotic potential toward the probiotic strain Lactobacillus plantarum 299v was demonstrated. The changes in physico-chemical and sensorial characteristics were esteemed as negligible.
PB  - Elsevier Ltd.
T2  - Food Chemistry
T1  - Production of prebiotic enriched maple syrup through enzymatic conversion of sucrose into fructo-oligosaccharides
SP  - 139180
VL  - 449
DO  - 10.1016/j.foodchem.2024.139180
ER  - 

@article{
author = "Veljković, Milica and Banjanac, Katarina and Milivojević, Ana and Ćorović, Marija and Simović, Milica and Bezbradica, Dejan",
year = "2024-08",
abstract = "Maple syrup, a popular natural sweetener has a high content of sucrose, whose consumption is linked to different health issues such as obesity and diabetes. Hence, within this paper, the conversion of sucrose to prebiotics (fructo-oligosaccharides, FOS) was proposed as a promising approach to obtaining a healthier, value-added product. Enzymatic conversion was optimized with respect to key experimental factors, and thereafter derived immobilized preparation of fructosyltransferase (FTase) from Pectinex® Ultra SP-L (FTase-epoxy Purolite, 255 IU/g support) was successfully utilized to produce novel functional product in ten consecutive reaction cycles. The product, obtained under optimal conditions (60 ◦C, 7.65 IU/mL, 12 h), resulted in 56.0% FOS, 16.7% sucrose, and 27.3% monosaccharides of total carbohydrates, leading to a 1.6-fold reduction in caloric content. The obtained products` prebiotic potential toward the probiotic strain Lactobacillus plantarum 299v was demonstrated. The changes in physico-chemical and sensorial characteristics were esteemed as negligible.",
publisher = "Elsevier Ltd.",
journal = "Food Chemistry",
title = "Production of prebiotic enriched maple syrup through enzymatic conversion of sucrose into fructo-oligosaccharides",
pages = "139180",
volume = "449",
doi = "10.1016/j.foodchem.2024.139180"
}

Veljković, M., Banjanac, K., Milivojević, A., Ćorović, M., Simović, M.,& Bezbradica, D.. (2024-08). Production of prebiotic enriched maple syrup through enzymatic conversion of sucrose into fructo-oligosaccharides. in Food Chemistry
Elsevier Ltd.., 449, 139180.
https://doi.org/10.1016/j.foodchem.2024.139180

Veljković M, Banjanac K, Milivojević A, Ćorović M, Simović M, Bezbradica D. Production of prebiotic enriched maple syrup through enzymatic conversion of sucrose into fructo-oligosaccharides. in Food Chemistry. 2024;449:139180.
doi:10.1016/j.foodchem.2024.139180 .

Veljković, Milica, Banjanac, Katarina, Milivojević, Ana, Ćorović, Marija, Simović, Milica, Bezbradica, Dejan, "Production of prebiotic enriched maple syrup through enzymatic conversion of sucrose into fructo-oligosaccharides" in Food Chemistry, 449 (2024-08):139180,
https://doi.org/10.1016/j.foodchem.2024.139180 . .

TY  - JOUR
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Veljković, Milica
AU  - Bezbradica, Dejan
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7384
AB  - Fruit pomaces are agro-industrial by-products obtained in large quantities by the juice production industry, hence their reutilization is a suitable way to minimize environmental impact by transforming them into different value-added products. Considering the wide abundance and bioactive potential of berries pomaces, raspberry, strawberry, blackcurrant, and chokeberry pomace were chosen to examine and compare polyphenol content, antioxidant activity, and skin prebiotic capacity of their extracts. Extracts of 5.28 to 13.39 mg GAE ⋅ g−1 DM total polyphenol content were obtained, with significant differences in polyphenol abundance between classes. Relative antioxidant capacity index and global antioxidant score revealed that the blackcurrant pomace extract exhibited the highest antioxidant capacity. Staphylococcus epidermidis growth was promoted by lower extract concentrations, while higher concentrations showed inhibitory effects. On the other hand, Staphylococcus aureus was inhibited more strongly and no stimulation was detected in the tested concentration range (.025–.1 mg GAE ⋅ mL−1). In co-cultured experiments, a S. aureus/S. epidermidis rebalancing effect was proven for all tested extracts, whereas the best prebiotic capacity of 2.84 was achieved at.05 mg GAE⋅ mL−1 of raspberry pomace extract. In conclusion, berry pomace extracts could be valorized by extraction of their phytochemicals which are potent antioxidants and emerging prebiotics for topical application.
PB  - Taylor and Francis Ltd.
T2  - International Journal of Fruit Science
T1  - Berries Pomace Valorization: From Waste to Potent Antioxidants and Emerging Skin Prebiotics
EP  - 101
IS  - 1
SP  - 85
VL  - 24
DO  - 10.1080/15538362.2024.2322743
ER  - 

@article{
author = "Petrov Ivanković, Anja and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Banjanac, Katarina and Veljković, Milica and Bezbradica, Dejan",
year = "2024",
abstract = "Fruit pomaces are agro-industrial by-products obtained in large quantities by the juice production industry, hence their reutilization is a suitable way to minimize environmental impact by transforming them into different value-added products. Considering the wide abundance and bioactive potential of berries pomaces, raspberry, strawberry, blackcurrant, and chokeberry pomace were chosen to examine and compare polyphenol content, antioxidant activity, and skin prebiotic capacity of their extracts. Extracts of 5.28 to 13.39 mg GAE ⋅ g−1 DM total polyphenol content were obtained, with significant differences in polyphenol abundance between classes. Relative antioxidant capacity index and global antioxidant score revealed that the blackcurrant pomace extract exhibited the highest antioxidant capacity. Staphylococcus epidermidis growth was promoted by lower extract concentrations, while higher concentrations showed inhibitory effects. On the other hand, Staphylococcus aureus was inhibited more strongly and no stimulation was detected in the tested concentration range (.025–.1 mg GAE ⋅ mL−1). In co-cultured experiments, a S. aureus/S. epidermidis rebalancing effect was proven for all tested extracts, whereas the best prebiotic capacity of 2.84 was achieved at.05 mg GAE⋅ mL−1 of raspberry pomace extract. In conclusion, berry pomace extracts could be valorized by extraction of their phytochemicals which are potent antioxidants and emerging prebiotics for topical application.",
publisher = "Taylor and Francis Ltd.",
journal = "International Journal of Fruit Science",
title = "Berries Pomace Valorization: From Waste to Potent Antioxidants and Emerging Skin Prebiotics",
pages = "101-85",
number = "1",
volume = "24",
doi = "10.1080/15538362.2024.2322743"
}

Petrov Ivanković, A., Ćorović, M., Milivojević, A., Simović, M., Banjanac, K., Veljković, M.,& Bezbradica, D.. (2024). Berries Pomace Valorization: From Waste to Potent Antioxidants and Emerging Skin Prebiotics. in International Journal of Fruit Science
Taylor and Francis Ltd.., 24(1), 85-101.
https://doi.org/10.1080/15538362.2024.2322743

Petrov Ivanković A, Ćorović M, Milivojević A, Simović M, Banjanac K, Veljković M, Bezbradica D. Berries Pomace Valorization: From Waste to Potent Antioxidants and Emerging Skin Prebiotics. in International Journal of Fruit Science. 2024;24(1):85-101.
doi:10.1080/15538362.2024.2322743 .

Petrov Ivanković, Anja, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Banjanac, Katarina, Veljković, Milica, Bezbradica, Dejan, "Berries Pomace Valorization: From Waste to Potent Antioxidants and Emerging Skin Prebiotics" in International Journal of Fruit Science, 24, no. 1 (2024):85-101,
https://doi.org/10.1080/15538362.2024.2322743 . .

TY  - JOUR
AU  - Prlainović, Nevena Ž.
AU  - Milovanović, Jelena S.
AU  - Milašinović, Nikola Z.
AU  - Bezbradica, Dejan I.
AU  - Mijin, Dušan Ž.
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7363
AB  - Lipase-catalyzed organic reactions have been widely practiced in the past three decades. Especially interesting are insoluble/immobilized forms due to providing a possibility of facile use and recyclability, thus reducing process costs, and making the procedure more environmentally friendly. Carbon-based supports have been extensively exploited for this purpose, because of neutral and biodegradable nature and thermal and chemical stability. Their high specific surface area, characteristic surface morphology and lower mass transfer resistances play a vital role in the performance of the attached enzyme. This review paper presents an overview of the main aspects of lipase immobilized on multi-walled carbon nanotubes (MWCNTs). Moreover, different immobilization strategies to achieve a biocatalyst with improved performances are discussed. Furthermore, as lipases are considered to have high commercial worth for synthesis of valuable organic molecules, the second part of the paper is dedicated to the overview of the most important industrial sectors in which these nanobiocatalysts have been used. In specific, applications in biodiesel production, flavour ester synthesis and racemization are summarized.
AB  - Lipaze su poslednjih decenija široko rasprostanjeni katalizatoriu raznovrsnim organskim reakcijama. Posebno su interesantne u imobilisanom/nerastvornom obliku jer je na ovaj način olakšana njihova upotreba uz mogućnost recikliranja i ponovne upotrebe čime se smanjuju troškovi samog procesa i postupak je ekološki prihvatljiviji. Kao nosači za vezivanje nanomaterijali na bazi ugljenika, posebno ugljenične nanocevi, su našli primenu zbog svojih izuzetnih fizičkih, mehaničkih i hemijskih svojstava. Njihova velika specifična površina, karakteristična površinska morfologija i smanjen otpor prenosu mase igraju vitalnu ulogu u performansama vezanog enzima. Ovaj pregledni rad predstavlja prikaz glavnih aspekata lipaze imobilisane na višeslojne ugljenične nanocevi i različitih strategija imobilizacije za dobijanje biokatalizatora sa poboljšanim svojstvima. Takođe, kako su lipaze enzimi od velikog komercijalnog značaja za organsku sintezu i primenu u biotehnologiji, drugi deo rada posvećen je pregledu najvažnijih industrijskih sektora u kojima su ovi nanobiokatalizatori našli primenu. Shodno tome, dat je pregled proizvodnje biodizela, mirisnih estara i racemizacije
PB  - Savez hemijskih inženjera Srbije
T2  - Hemijska industrija
T1  - Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update
T1  - Višeslojne ugljenične nanocevi kao nosač lipaze za organsku sintezu: pregled najnovijih trendova
EP  - 16
IS  - 1
SP  - 1
VL  - 78
DO  - 10.2298/HEMIND230614003P
ER  - 

@article{
author = "Prlainović, Nevena Ž. and Milovanović, Jelena S. and Milašinović, Nikola Z. and Bezbradica, Dejan I. and Mijin, Dušan Ž.",
year = "2024",
abstract = "Lipase-catalyzed organic reactions have been widely practiced in the past three decades. Especially interesting are insoluble/immobilized forms due to providing a possibility of facile use and recyclability, thus reducing process costs, and making the procedure more environmentally friendly. Carbon-based supports have been extensively exploited for this purpose, because of neutral and biodegradable nature and thermal and chemical stability. Their high specific surface area, characteristic surface morphology and lower mass transfer resistances play a vital role in the performance of the attached enzyme. This review paper presents an overview of the main aspects of lipase immobilized on multi-walled carbon nanotubes (MWCNTs). Moreover, different immobilization strategies to achieve a biocatalyst with improved performances are discussed. Furthermore, as lipases are considered to have high commercial worth for synthesis of valuable organic molecules, the second part of the paper is dedicated to the overview of the most important industrial sectors in which these nanobiocatalysts have been used. In specific, applications in biodiesel production, flavour ester synthesis and racemization are summarized., Lipaze su poslednjih decenija široko rasprostanjeni katalizatoriu raznovrsnim organskim reakcijama. Posebno su interesantne u imobilisanom/nerastvornom obliku jer je na ovaj način olakšana njihova upotreba uz mogućnost recikliranja i ponovne upotrebe čime se smanjuju troškovi samog procesa i postupak je ekološki prihvatljiviji. Kao nosači za vezivanje nanomaterijali na bazi ugljenika, posebno ugljenične nanocevi, su našli primenu zbog svojih izuzetnih fizičkih, mehaničkih i hemijskih svojstava. Njihova velika specifična površina, karakteristična površinska morfologija i smanjen otpor prenosu mase igraju vitalnu ulogu u performansama vezanog enzima. Ovaj pregledni rad predstavlja prikaz glavnih aspekata lipaze imobilisane na višeslojne ugljenične nanocevi i različitih strategija imobilizacije za dobijanje biokatalizatora sa poboljšanim svojstvima. Takođe, kako su lipaze enzimi od velikog komercijalnog značaja za organsku sintezu i primenu u biotehnologiji, drugi deo rada posvećen je pregledu najvažnijih industrijskih sektora u kojima su ovi nanobiokatalizatori našli primenu. Shodno tome, dat je pregled proizvodnje biodizela, mirisnih estara i racemizacije",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija",
title = "Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update, Višeslojne ugljenične nanocevi kao nosač lipaze za organsku sintezu: pregled najnovijih trendova",
pages = "16-1",
number = "1",
volume = "78",
doi = "10.2298/HEMIND230614003P"
}

Prlainović, N. Ž., Milovanović, J. S., Milašinović, N. Z., Bezbradica, D. I.,& Mijin, D. Ž.. (2024). Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update. in Hemijska industrija
Savez hemijskih inženjera Srbije., 78(1), 1-16.
https://doi.org/10.2298/HEMIND230614003P

Prlainović NŽ, Milovanović JS, Milašinović NZ, Bezbradica DI, Mijin DŽ. Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update. in Hemijska industrija. 2024;78(1):1-16.
doi:10.2298/HEMIND230614003P .

Prlainović, Nevena Ž., Milovanović, Jelena S., Milašinović, Nikola Z., Bezbradica, Dejan I., Mijin, Dušan Ž., "Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update" in Hemijska industrija, 78, no. 1 (2024):1-16,
https://doi.org/10.2298/HEMIND230614003P . .

TY  - JOUR
AU  - Jevtović, Đorđe
AU  - Živković, Predrag
AU  - Milivojević, Ana
AU  - Bezbradica, Dejan
AU  - Van Der Auwera, Luc
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6987
AB  - Due to the high wastepaper recyclability and water-loop system closure, packaging paper mills struggle with increased fines, causing runnability issues. Cellulase enzymes are a preferred treatment choice for the improvement of the pulp refining in stock preparation area but are not widely used or easy to introduce in the production process. Different cellulase enzymes were tested, and those with the highest activity were introduced to the white-water (WW) samples with the aim to reduce fines content as potentially new enzyme applications on the paper machine. The first portion of the study involved the development of an experiment model to find and confirm the optimal enzyme process parameters (40 °C, pH 5.7, reaction time 3 h, and 0.18% v/v enzyme addition) for laboratory made white-water. The second portion of the study included turbidity, colloidal charge, flow cytometry (FCM), and chemical oxygen demand (COD) analysis on industrial and laboratory made white-water samples at optimized process parameters. Obtained results corresponded to reduced fines content in white-water samples, which justified commercial usage of cellulase enzymes on recycled paper machine short loop and potentially increased machine runnability without negative influence on wastewater treatment plant.
PB  - North Carolina State University
T2  - BioResources
T1  - Reduction of fines in recycled paper white water via cellulase enzymes
EP  - 655
IS  - 1
SP  - 635
VL  - 19
DO  - 10.15376/biores.19.1.635-655
ER  - 

@article{
author = "Jevtović, Đorđe and Živković, Predrag and Milivojević, Ana and Bezbradica, Dejan and Van Der Auwera, Luc",
year = "2024",
abstract = "Due to the high wastepaper recyclability and water-loop system closure, packaging paper mills struggle with increased fines, causing runnability issues. Cellulase enzymes are a preferred treatment choice for the improvement of the pulp refining in stock preparation area but are not widely used or easy to introduce in the production process. Different cellulase enzymes were tested, and those with the highest activity were introduced to the white-water (WW) samples with the aim to reduce fines content as potentially new enzyme applications on the paper machine. The first portion of the study involved the development of an experiment model to find and confirm the optimal enzyme process parameters (40 °C, pH 5.7, reaction time 3 h, and 0.18% v/v enzyme addition) for laboratory made white-water. The second portion of the study included turbidity, colloidal charge, flow cytometry (FCM), and chemical oxygen demand (COD) analysis on industrial and laboratory made white-water samples at optimized process parameters. Obtained results corresponded to reduced fines content in white-water samples, which justified commercial usage of cellulase enzymes on recycled paper machine short loop and potentially increased machine runnability without negative influence on wastewater treatment plant.",
publisher = "North Carolina State University",
journal = "BioResources",
title = "Reduction of fines in recycled paper white water via cellulase enzymes",
pages = "655-635",
number = "1",
volume = "19",
doi = "10.15376/biores.19.1.635-655"
}

Jevtović, Đ., Živković, P., Milivojević, A., Bezbradica, D.,& Van Der Auwera, L.. (2024). Reduction of fines in recycled paper white water via cellulase enzymes. in BioResources
North Carolina State University., 19(1), 635-655.
https://doi.org/10.15376/biores.19.1.635-655

Jevtović Đ, Živković P, Milivojević A, Bezbradica D, Van Der Auwera L. Reduction of fines in recycled paper white water via cellulase enzymes. in BioResources. 2024;19(1):635-655.
doi:10.15376/biores.19.1.635-655 .

Jevtović, Đorđe, Živković, Predrag, Milivojević, Ana, Bezbradica, Dejan, Van Der Auwera, Luc, "Reduction of fines in recycled paper white water via cellulase enzymes" in BioResources, 19, no. 1 (2024):635-655,
https://doi.org/10.15376/biores.19.1.635-655 . .

TY  - CONF
AU  - Vukoičić, Ana
AU  - Milivojević, Ana
AU  - Banjanac, Katarina
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Marinković, Aleksandar
AU  - Palomo, Jose Miguel
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6844
AB  - Flavonoids are a big class of plants’ secondary metabolites which possess antimicrobial, antioxidative, anti-inflammatory, etc. activities. However, their wider application is limited due to their poor solubility and stability in different environments. In order to overcome this problem and improve their biological and physicochemical characteristics, enzymatic oligomerization of flavonoids can be performed by using laccases as biocatalyst. In this study, laccase from Trametes versicolor was successfully immobilized onto two nanocarriers and used as a catalyst for the oligomerization of two flavonoids, esculin and phloridzin. For immobilization, nanocelulose prepared by ultrasonic treatment of commercial microcellulose and nanosilica from rice were used. Both carriers were modified in order to introduce amino groups on their surface: nanocelulose with poly(ethyleneimine) (NC-PEI) and nanosilica with organosylane (NS-OS). Most active preparation for NC-PEIlaccase and NS-OS-laccase showed activities of 10.05 IU/g of support and 9.08 IU/g of support, respectively. The oligomerization of flavonoids was performed using immobilized laccase and obtained results were compared with those obtained with free laccase (same amount of activity units) as biocatalyst. Immobilized preparations showed promising activities in reaction of esculin oligomerization, since after 24h of reaction the conversions of 73.3% and 61.6% with NC-PEI-laccase and NS-OS-laccase, respectively was achieved, while the soluble enzyme gave the conversion of 92.4%. On the other hand, some lower activity of NC-PEI-laccase was observed in reaction of phloridzin oligomerization, since the achieved conversion was 43.3% compared to 80.7% of phloridzin conversion obtained with soluble laccase. The lower activity could be explained by different reaction medium, since, compared to the esculin oligomerization, the phloridzin oligomerization was performed in 30% methanol, due to its low water solubility. However, obtained results indicate that novel immobilized laccase preparations can be successfully applied in the production of various flavonoid oligomers.
PB  - Belgrade : University, Faculty of Geography
C3  - Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023
T1  - Immobilization of laccase from Trametes versicolor on different nanocarriers and its application in the synthesis of flavonoid oligomers
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6844
ER  - 

@conference{
author = "Vukoičić, Ana and Milivojević, Ana and Banjanac, Katarina and Petrov Ivanković, Anja and Ćorović, Marija and Marinković, Aleksandar and Palomo, Jose Miguel and Bezbradica, Dejan",
year = "2023",
abstract = "Flavonoids are a big class of plants’ secondary metabolites which possess antimicrobial, antioxidative, anti-inflammatory, etc. activities. However, their wider application is limited due to their poor solubility and stability in different environments. In order to overcome this problem and improve their biological and physicochemical characteristics, enzymatic oligomerization of flavonoids can be performed by using laccases as biocatalyst. In this study, laccase from Trametes versicolor was successfully immobilized onto two nanocarriers and used as a catalyst for the oligomerization of two flavonoids, esculin and phloridzin. For immobilization, nanocelulose prepared by ultrasonic treatment of commercial microcellulose and nanosilica from rice were used. Both carriers were modified in order to introduce amino groups on their surface: nanocelulose with poly(ethyleneimine) (NC-PEI) and nanosilica with organosylane (NS-OS). Most active preparation for NC-PEIlaccase and NS-OS-laccase showed activities of 10.05 IU/g of support and 9.08 IU/g of support, respectively. The oligomerization of flavonoids was performed using immobilized laccase and obtained results were compared with those obtained with free laccase (same amount of activity units) as biocatalyst. Immobilized preparations showed promising activities in reaction of esculin oligomerization, since after 24h of reaction the conversions of 73.3% and 61.6% with NC-PEI-laccase and NS-OS-laccase, respectively was achieved, while the soluble enzyme gave the conversion of 92.4%. On the other hand, some lower activity of NC-PEI-laccase was observed in reaction of phloridzin oligomerization, since the achieved conversion was 43.3% compared to 80.7% of phloridzin conversion obtained with soluble laccase. The lower activity could be explained by different reaction medium, since, compared to the esculin oligomerization, the phloridzin oligomerization was performed in 30% methanol, due to its low water solubility. However, obtained results indicate that novel immobilized laccase preparations can be successfully applied in the production of various flavonoid oligomers.",
publisher = "Belgrade : University, Faculty of Geography",
journal = "Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023",
title = "Immobilization of laccase from Trametes versicolor on different nanocarriers and its application in the synthesis of flavonoid oligomers",
pages = "27",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6844"
}

Vukoičić, A., Milivojević, A., Banjanac, K., Petrov Ivanković, A., Ćorović, M., Marinković, A., Palomo, J. M.,& Bezbradica, D.. (2023). Immobilization of laccase from Trametes versicolor on different nanocarriers and its application in the synthesis of flavonoid oligomers. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023
Belgrade : University, Faculty of Geography., 27.
https://hdl.handle.net/21.15107/rcub_technorep_6844

Vukoičić A, Milivojević A, Banjanac K, Petrov Ivanković A, Ćorović M, Marinković A, Palomo JM, Bezbradica D. Immobilization of laccase from Trametes versicolor on different nanocarriers and its application in the synthesis of flavonoid oligomers. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023. 2023;:27.
https://hdl.handle.net/21.15107/rcub_technorep_6844 .

Vukoičić, Ana, Milivojević, Ana, Banjanac, Katarina, Petrov Ivanković, Anja, Ćorović, Marija, Marinković, Aleksandar, Palomo, Jose Miguel, Bezbradica, Dejan, "Immobilization of laccase from Trametes versicolor on different nanocarriers and its application in the synthesis of flavonoid oligomers" in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023 (2023):27,
https://hdl.handle.net/21.15107/rcub_technorep_6844 .

TY  - JOUR
AU  - Salih, Rabab
AU  - Banjanac, Katarina
AU  - Vukoičić, Ana
AU  - Gržetić, Jelena
AU  - Popović, Ana
AU  - Veljković, Milica
AU  - Bezbradica, Dejan
AU  - Marinković, Aleksandar
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5278
AB  - The novel lignin-based microspheres (LMS-DEGDMA) were produced through a two-step modification procedure consisting of kraft lignin (KL) modification with acrylic acid synthesized from fructose; and suspension and copolymerization of acrylic acid modified KL (KL-A) with diethylene glycol dimethacrylate (DEGDMA). The applied procedure provided LMS-DEGDMA microspheres in the size range of 50-90 μm with specific surface area of 39 m2/g, porosity ϵp 62, pore diameters of 4-20 nm and total pore volume of 0.14 cm3/g. Immobilization of laccase from Myceliophthora thermophilia expressed in Aspergillus oryzae (Novozyme® 51003) on LMS-DEGDMA was explored. By optimization of the immobilization process, it was demonstrated that laccase immobilization on LMS-DEGDMA exhibited the best results (protein immobilization yield of 70 %, activity yield of 27 %, and catalytic activity of 262 IU/g of support) when the immobilization was carried out at pH 5.0 and 100 mg of offered protein per g support during 1 h. Additionally, the LMS-DEGDMA-laccase preparation showed efficiency (>80 %, 5-7 cycles) in decolorization of anthraquinone dyes (Lanaset® violet B, Lanaset® blue 2 R and C.I. Acid Green 40) and their mixture. In case of Lanaset® violet B, LMS-DEGDMA improved laccase's kinetics characteristics (90 % over 24 h) along with operational stability (> 63 % catalytic activity, 7 cycles).
PB  - Elsevier Ltd.
T2  - Journal of Environmental Chemical Engineering
T1  - Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes
IS  - 1
SP  - 109077
VL  - 11
DO  - 10.1016/j.jece.2022.109077
ER  - 

@article{
author = "Salih, Rabab and Banjanac, Katarina and Vukoičić, Ana and Gržetić, Jelena and Popović, Ana and Veljković, Milica and Bezbradica, Dejan and Marinković, Aleksandar",
year = "2023",
abstract = "The novel lignin-based microspheres (LMS-DEGDMA) were produced through a two-step modification procedure consisting of kraft lignin (KL) modification with acrylic acid synthesized from fructose; and suspension and copolymerization of acrylic acid modified KL (KL-A) with diethylene glycol dimethacrylate (DEGDMA). The applied procedure provided LMS-DEGDMA microspheres in the size range of 50-90 μm with specific surface area of 39 m2/g, porosity ϵp 62, pore diameters of 4-20 nm and total pore volume of 0.14 cm3/g. Immobilization of laccase from Myceliophthora thermophilia expressed in Aspergillus oryzae (Novozyme® 51003) on LMS-DEGDMA was explored. By optimization of the immobilization process, it was demonstrated that laccase immobilization on LMS-DEGDMA exhibited the best results (protein immobilization yield of 70 %, activity yield of 27 %, and catalytic activity of 262 IU/g of support) when the immobilization was carried out at pH 5.0 and 100 mg of offered protein per g support during 1 h. Additionally, the LMS-DEGDMA-laccase preparation showed efficiency (>80 %, 5-7 cycles) in decolorization of anthraquinone dyes (Lanaset® violet B, Lanaset® blue 2 R and C.I. Acid Green 40) and their mixture. In case of Lanaset® violet B, LMS-DEGDMA improved laccase's kinetics characteristics (90 % over 24 h) along with operational stability (> 63 % catalytic activity, 7 cycles).",
publisher = "Elsevier Ltd.",
journal = "Journal of Environmental Chemical Engineering",
title = "Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes",
number = "1",
pages = "109077",
volume = "11",
doi = "10.1016/j.jece.2022.109077"
}

Salih, R., Banjanac, K., Vukoičić, A., Gržetić, J., Popović, A., Veljković, M., Bezbradica, D.,& Marinković, A.. (2023). Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes. in Journal of Environmental Chemical Engineering
Elsevier Ltd.., 11(1), 109077.
https://doi.org/10.1016/j.jece.2022.109077

Salih R, Banjanac K, Vukoičić A, Gržetić J, Popović A, Veljković M, Bezbradica D, Marinković A. Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes. in Journal of Environmental Chemical Engineering. 2023;11(1):109077.
doi:10.1016/j.jece.2022.109077 .

Salih, Rabab, Banjanac, Katarina, Vukoičić, Ana, Gržetić, Jelena, Popović, Ana, Veljković, Milica, Bezbradica, Dejan, Marinković, Aleksandar, "Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes" in Journal of Environmental Chemical Engineering, 11, no. 1 (2023):109077,
https://doi.org/10.1016/j.jece.2022.109077 . .

TY  - CONF
AU  - Petrov Ivanković, Anja
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Veljković, Milica
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7091
AB  - Phloridzin is flavonoid known for its antioxidant and UV-protective effects on the skin. However, it has limited solubility in both hydrophilic and lipophilic media, therefore it should be derivatized prior to incorporation in final products. Hereby, we applied lipase-catalyzed acylation to produce different phloridzin ester mixtures using coconut and linseed oil as acyl donors and examined their transdermal delivery. The new derivatives were incorporated in oil in water emulsion to examine their diffusion through artificial skin-mimicking Strat-M® membrane using Franz diffusion cell. The transport of esters was successfully modelled by modified Kosrmeyer-Pepas equation. Lag phase, lasting 90-170 min, was detected for all esters. For coconut oil-derived esters process was entirely controlled by diffusion, while for linseed oil-based esters polymer relaxation was observed, as well. Effective diffusion coefficients of medium-chain esters obtained using coconut oil were one order of magnitude higher compared to long-chain ones. Zeta potential of both formulations was constant during six month storage period, indicating their high stability. Obtained results underscore suitability of natural triglycerides application in the production of phloridzin esters for topical products.
PB  - Sciendo
C3  - The EuroBiotech Journal
T1  - Transdermal diffusion of enzymatically synthesized oil-derived phloridzin esters from oil in water emulsion
IS  - S1
SP  - 18
VL  - 7
DO  - 10.2478/ebtj-2023-0018
ER  - 

@conference{
author = "Petrov Ivanković, Anja and Milivojević, Ana and Ćorović, Marija and Veljković, Milica and Bezbradica, Dejan",
year = "2023",
abstract = "Phloridzin is flavonoid known for its antioxidant and UV-protective effects on the skin. However, it has limited solubility in both hydrophilic and lipophilic media, therefore it should be derivatized prior to incorporation in final products. Hereby, we applied lipase-catalyzed acylation to produce different phloridzin ester mixtures using coconut and linseed oil as acyl donors and examined their transdermal delivery. The new derivatives were incorporated in oil in water emulsion to examine their diffusion through artificial skin-mimicking Strat-M® membrane using Franz diffusion cell. The transport of esters was successfully modelled by modified Kosrmeyer-Pepas equation. Lag phase, lasting 90-170 min, was detected for all esters. For coconut oil-derived esters process was entirely controlled by diffusion, while for linseed oil-based esters polymer relaxation was observed, as well. Effective diffusion coefficients of medium-chain esters obtained using coconut oil were one order of magnitude higher compared to long-chain ones. Zeta potential of both formulations was constant during six month storage period, indicating their high stability. Obtained results underscore suitability of natural triglycerides application in the production of phloridzin esters for topical products.",
publisher = "Sciendo",
journal = "The EuroBiotech Journal",
title = "Transdermal diffusion of enzymatically synthesized oil-derived phloridzin esters from oil in water emulsion",
number = "S1",
pages = "18",
volume = "7",
doi = "10.2478/ebtj-2023-0018"
}

Petrov Ivanković, A., Milivojević, A., Ćorović, M., Veljković, M.,& Bezbradica, D.. (2023). Transdermal diffusion of enzymatically synthesized oil-derived phloridzin esters from oil in water emulsion. in The EuroBiotech Journal
Sciendo., 7(S1), 18.
https://doi.org/10.2478/ebtj-2023-0018

Petrov Ivanković A, Milivojević A, Ćorović M, Veljković M, Bezbradica D. Transdermal diffusion of enzymatically synthesized oil-derived phloridzin esters from oil in water emulsion. in The EuroBiotech Journal. 2023;7(S1):18.
doi:10.2478/ebtj-2023-0018 .

Petrov Ivanković, Anja, Milivojević, Ana, Ćorović, Marija, Veljković, Milica, Bezbradica, Dejan, "Transdermal diffusion of enzymatically synthesized oil-derived phloridzin esters from oil in water emulsion" in The EuroBiotech Journal, 7, no. S1 (2023):18,
https://doi.org/10.2478/ebtj-2023-0018 . .

TY  - JOUR
AU  - Veljković, Milica
AU  - Stepanović, Relja
AU  - Banjanac, Katarina
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Milivojević, Milan
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5247
AB  - Within this study, the investigation of potential selective immobilization of fructosyltransferase (FTase) derived from Aspergillus aculeatus (from commercial enzyme preparation Pectinex® Ultra SP-L) onto the polystyrenic macroporous resin Purolite® A109 and its further utilization for the synthesis of a highly valuable group of prebiotic compounds named fructo-oligosaccharides (FOS), was performed. The obtained results suggest that partial purification of fructosyltransferase from complex enzyme mixture with predominant pectinase activity (ratio of activity immobilization yields around 20.0) occurred by performing a simple immobilization protocol, provided that immobilization lasted for 5 h using an enzyme concentration of 50 mg g−1 of support under slightly acidic conditions (pH 6.0). Immobilized preparation was thereafter successfully utilized for FOS synthesis (56–59 % FOS of total sugars) in both a batch and air-lift reactor system. Operational stability study proved the exceptionally high potential of immobilized preparation for reproducible and cost-effective application, since 90 % of initial activity was preserved after ten consecutive reaction cycles. Thereafter, a prospectus for continuous production of FOS in the air-lift reactor was assayed. Achieved yields, 53 % FOS of total sugars for at least 9 h, indicated the great suitability of the obtained immobilized preparation for application in large-scale systems.
PB  - Korean Society of Industrial Engineering Chemistry
T2  - Journal of Industrial and Engineering Chemistry
T1  - Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109
EP  - 156
SP  - 149
VL  - 117
DO  - 10.1016/j.jiec.2022.09.051
ER  - 

@article{
author = "Veljković, Milica and Stepanović, Relja and Banjanac, Katarina and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Milivojević, Milan and Bezbradica, Dejan",
year = "2023",
abstract = "Within this study, the investigation of potential selective immobilization of fructosyltransferase (FTase) derived from Aspergillus aculeatus (from commercial enzyme preparation Pectinex® Ultra SP-L) onto the polystyrenic macroporous resin Purolite® A109 and its further utilization for the synthesis of a highly valuable group of prebiotic compounds named fructo-oligosaccharides (FOS), was performed. The obtained results suggest that partial purification of fructosyltransferase from complex enzyme mixture with predominant pectinase activity (ratio of activity immobilization yields around 20.0) occurred by performing a simple immobilization protocol, provided that immobilization lasted for 5 h using an enzyme concentration of 50 mg g−1 of support under slightly acidic conditions (pH 6.0). Immobilized preparation was thereafter successfully utilized for FOS synthesis (56–59 % FOS of total sugars) in both a batch and air-lift reactor system. Operational stability study proved the exceptionally high potential of immobilized preparation for reproducible and cost-effective application, since 90 % of initial activity was preserved after ten consecutive reaction cycles. Thereafter, a prospectus for continuous production of FOS in the air-lift reactor was assayed. Achieved yields, 53 % FOS of total sugars for at least 9 h, indicated the great suitability of the obtained immobilized preparation for application in large-scale systems.",
publisher = "Korean Society of Industrial Engineering Chemistry",
journal = "Journal of Industrial and Engineering Chemistry",
title = "Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109",
pages = "156-149",
volume = "117",
doi = "10.1016/j.jiec.2022.09.051"
}

Veljković, M., Stepanović, R., Banjanac, K., Ćorović, M., Milivojević, A., Simović, M., Milivojević, M.,& Bezbradica, D.. (2023). Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109. in Journal of Industrial and Engineering Chemistry
Korean Society of Industrial Engineering Chemistry., 117, 149-156.
https://doi.org/10.1016/j.jiec.2022.09.051

Veljković M, Stepanović R, Banjanac K, Ćorović M, Milivojević A, Simović M, Milivojević M, Bezbradica D. Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109. in Journal of Industrial and Engineering Chemistry. 2023;117:149-156.
doi:10.1016/j.jiec.2022.09.051 .

Veljković, Milica, Stepanović, Relja, Banjanac, Katarina, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Milivojević, Milan, Bezbradica, Dejan, "Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109" in Journal of Industrial and Engineering Chemistry, 117 (2023):149-156,
https://doi.org/10.1016/j.jiec.2022.09.051 . .

TY  - JOUR
AU  - Andreu, Alicia
AU  - Ćorović, Marija
AU  - Garcia-Sanz, Carla
AU  - Santos, A. Sofia
AU  - Milivojević, Ana
AU  - Ortega-Nieto, Clara
AU  - Mateo, Cesar
AU  - Bezbradica, Dejan
AU  - Palomo, Jose M.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6804
AB  - Enzymatic glycosylation is a versatile and sustainable biotechnological approach that plays a pivotal role in the production of bioactive compounds. This process involves the enzymatic transfer of sugar moieties onto various acceptor molecules, such as small molecules, peptides, or proteins, resulting in the synthesis of glycosides. These glycosides often exhibit enhanced bioactivity, improved solubility, and enhanced stability, making them valuable in pharmaceuticals, nutraceuticals, and the food industry. This review explores the diverse enzymatic glycosylation strategies employed in the synthesis of bioactive compounds. It highlights the enzymatic catalysts involved, including glycosyltransferases, glycosidases, glycophosphorylases, and glycosynthases. It considers the advantages and disadvantages of these biocatalysts in the stereoselective and regioselective synthesis of different types of glycosylated molecules, phenolic and aliphatic alcohols, oligosaccharides, polysaccharides, glycoderivatives, glycopeptides, and glycoproteins with a clear focus on food and pharmaceutical chemistry. Furthermore, the review outlines various sources of sugar donors, activated glycosides, and sugar nucleotides, as well as the utilization of engineered enzymes and microorganisms for glycosylation reactions. The advantages of enzymatic glycosylation, including its high regioselectivity, stereoselectivity, and sustainability, are emphasized. Therefore, these approaches combining the use of different catalytic systems, the improvement of tools such as immobilization technology or chemical or genetic modification to improve the glycosylation process, could be useful tools in continuous biotechnological advancements.
PB  - MDPI
T2  - Catalysts
T1  - Enzymatic Glycosylation Strategies in the Production of Bioactive Compounds
IS  - 10
SP  - 1359
VL  - 13
DO  - 10.3390/catal13101359
ER  - 

@article{
author = "Andreu, Alicia and Ćorović, Marija and Garcia-Sanz, Carla and Santos, A. Sofia and Milivojević, Ana and Ortega-Nieto, Clara and Mateo, Cesar and Bezbradica, Dejan and Palomo, Jose M.",
year = "2023",
abstract = "Enzymatic glycosylation is a versatile and sustainable biotechnological approach that plays a pivotal role in the production of bioactive compounds. This process involves the enzymatic transfer of sugar moieties onto various acceptor molecules, such as small molecules, peptides, or proteins, resulting in the synthesis of glycosides. These glycosides often exhibit enhanced bioactivity, improved solubility, and enhanced stability, making them valuable in pharmaceuticals, nutraceuticals, and the food industry. This review explores the diverse enzymatic glycosylation strategies employed in the synthesis of bioactive compounds. It highlights the enzymatic catalysts involved, including glycosyltransferases, glycosidases, glycophosphorylases, and glycosynthases. It considers the advantages and disadvantages of these biocatalysts in the stereoselective and regioselective synthesis of different types of glycosylated molecules, phenolic and aliphatic alcohols, oligosaccharides, polysaccharides, glycoderivatives, glycopeptides, and glycoproteins with a clear focus on food and pharmaceutical chemistry. Furthermore, the review outlines various sources of sugar donors, activated glycosides, and sugar nucleotides, as well as the utilization of engineered enzymes and microorganisms for glycosylation reactions. The advantages of enzymatic glycosylation, including its high regioselectivity, stereoselectivity, and sustainability, are emphasized. Therefore, these approaches combining the use of different catalytic systems, the improvement of tools such as immobilization technology or chemical or genetic modification to improve the glycosylation process, could be useful tools in continuous biotechnological advancements.",
publisher = "MDPI",
journal = "Catalysts",
title = "Enzymatic Glycosylation Strategies in the Production of Bioactive Compounds",
number = "10",
pages = "1359",
volume = "13",
doi = "10.3390/catal13101359"
}

Andreu, A., Ćorović, M., Garcia-Sanz, C., Santos, A. S., Milivojević, A., Ortega-Nieto, C., Mateo, C., Bezbradica, D.,& Palomo, J. M.. (2023). Enzymatic Glycosylation Strategies in the Production of Bioactive Compounds. in Catalysts
MDPI., 13(10), 1359.
https://doi.org/10.3390/catal13101359

Andreu A, Ćorović M, Garcia-Sanz C, Santos AS, Milivojević A, Ortega-Nieto C, Mateo C, Bezbradica D, Palomo JM. Enzymatic Glycosylation Strategies in the Production of Bioactive Compounds. in Catalysts. 2023;13(10):1359.
doi:10.3390/catal13101359 .

Andreu, Alicia, Ćorović, Marija, Garcia-Sanz, Carla, Santos, A. Sofia, Milivojević, Ana, Ortega-Nieto, Clara, Mateo, Cesar, Bezbradica, Dejan, Palomo, Jose M., "Enzymatic Glycosylation Strategies in the Production of Bioactive Compounds" in Catalysts, 13, no. 10 (2023):1359,
https://doi.org/10.3390/catal13101359 . .

TY  - JOUR
AU  - Petrov Ivanković, Anja
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Jansen, Patrick
AU  - Vukoičić, Ana
AU  - van den Bogaard, Ellen
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6870
AB  - Background Blackcurrant is a rich source of polyphenols with proven physiological activity, that have lately been classified as emerging prebiotics, making its extracts suitable for application in functional food and bioactive cosmetics. However, these biomolecules are trapped in cell wall structures and nowadays, enzymatic hydrolysis is recognised as powerful tool for extraction efficiency improvement in an environmentally sound manner. This study aimed to optimize enzyme-assisted extraction to maximize yields of blackcurrant polyphenols. In addition, their prebiotic potential was tested by examining their influence on typical skin microbiota representatives as model microorganisms—beneficial coagulase-negative Staphylococcus epidermidis and two opportunistic pathogens, Staphylococcus aureus and Cutibacterium acnes. Results Among four examined commercial enzyme preparations, Viscozyme® L and Rohapect® MC, demonstrated the highest potential as extraction enhancers by increasing antioxidant activity, total polyphenol, and flavonoid contents. Furthermore, their synergetic effect enabled 95.3% increase in polyphenol concentration compared to conventional extraction with a maximum of 27.6 mg gallic acid equivalents (GAE)/g dry matter (DM) at a total enzyme concentration of 0.05 mL/g DM (Viscozyme® L to Rohapect® MC ratio 2:1) after 60 min. HPLC profiles of two samples showed differences in polyphenol composition indicating simultaneous extraction–biotransformation. At a concentration of 0.025 mg GAE/mL, PAS (prebiotic activity score) values were 0.096 and 0.172 for conventionally and enzymatically obtained extract, respectively. Moderate inhibitory effect of optimal extract concentration on Cutibacterium acnes was shown, as well. Hydrogel prepared with optimal extract concentration showed improved hydration effect, decreased irritability and increased dermatological compatibility comparing to basic formulation. Conclusions Overall results demonstrated that blackcurrant extract obtained under optimized conditions using enzymes possesses an emerging prebiotic potential for selective skin microbiota stimulation.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Chemical and Biological Technologies in Agriculture
T1  - In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives
IS  - 1
SP  - 125
VL  - 10
DO  - 10.1186/s40538-023-00502-8
ER  - 

@article{
author = "Petrov Ivanković, Anja and Milivojević, Ana and Ćorović, Marija and Simović, Milica and Banjanac, Katarina and Jansen, Patrick and Vukoičić, Ana and van den Bogaard, Ellen and Bezbradica, Dejan",
year = "2023",
abstract = "Background Blackcurrant is a rich source of polyphenols with proven physiological activity, that have lately been classified as emerging prebiotics, making its extracts suitable for application in functional food and bioactive cosmetics. However, these biomolecules are trapped in cell wall structures and nowadays, enzymatic hydrolysis is recognised as powerful tool for extraction efficiency improvement in an environmentally sound manner. This study aimed to optimize enzyme-assisted extraction to maximize yields of blackcurrant polyphenols. In addition, their prebiotic potential was tested by examining their influence on typical skin microbiota representatives as model microorganisms—beneficial coagulase-negative Staphylococcus epidermidis and two opportunistic pathogens, Staphylococcus aureus and Cutibacterium acnes. Results Among four examined commercial enzyme preparations, Viscozyme® L and Rohapect® MC, demonstrated the highest potential as extraction enhancers by increasing antioxidant activity, total polyphenol, and flavonoid contents. Furthermore, their synergetic effect enabled 95.3% increase in polyphenol concentration compared to conventional extraction with a maximum of 27.6 mg gallic acid equivalents (GAE)/g dry matter (DM) at a total enzyme concentration of 0.05 mL/g DM (Viscozyme® L to Rohapect® MC ratio 2:1) after 60 min. HPLC profiles of two samples showed differences in polyphenol composition indicating simultaneous extraction–biotransformation. At a concentration of 0.025 mg GAE/mL, PAS (prebiotic activity score) values were 0.096 and 0.172 for conventionally and enzymatically obtained extract, respectively. Moderate inhibitory effect of optimal extract concentration on Cutibacterium acnes was shown, as well. Hydrogel prepared with optimal extract concentration showed improved hydration effect, decreased irritability and increased dermatological compatibility comparing to basic formulation. Conclusions Overall results demonstrated that blackcurrant extract obtained under optimized conditions using enzymes possesses an emerging prebiotic potential for selective skin microbiota stimulation.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Chemical and Biological Technologies in Agriculture",
title = "In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives",
number = "1",
pages = "125",
volume = "10",
doi = "10.1186/s40538-023-00502-8"
}

Petrov Ivanković, A., Milivojević, A., Ćorović, M., Simović, M., Banjanac, K., Jansen, P., Vukoičić, A., van den Bogaard, E.,& Bezbradica, D.. (2023). In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives. in Chemical and Biological Technologies in Agriculture
Springer Science and Business Media Deutschland GmbH., 10(1), 125.
https://doi.org/10.1186/s40538-023-00502-8

Petrov Ivanković A, Milivojević A, Ćorović M, Simović M, Banjanac K, Jansen P, Vukoičić A, van den Bogaard E, Bezbradica D. In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives. in Chemical and Biological Technologies in Agriculture. 2023;10(1):125.
doi:10.1186/s40538-023-00502-8 .

Petrov Ivanković, Anja, Milivojević, Ana, Ćorović, Marija, Simović, Milica, Banjanac, Katarina, Jansen, Patrick, Vukoičić, Ana, van den Bogaard, Ellen, Bezbradica, Dejan, "In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives" in Chemical and Biological Technologies in Agriculture, 10, no. 1 (2023):125,
https://doi.org/10.1186/s40538-023-00502-8 . .

TY  - CONF
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6962
AB  - Fructo-oligosaccharides (FOS) are linear bioactive molecules consisting of one
terminal glucose and other fructose units and belong to the group of prebiotics with
scientifically proven benefits for human health. Physiological active compounds like
trisaccharides (FOS3), tetrasaccharides (FOS4) and pentasaccharides (FOS5) can be
produced from sucrose using enzyme-fructosyltransferase. The reaction mixture obtained in
this way contains, in addition to the desired prebiotics, glucose, fructose and an unreacted
amount of sucrose. Direct incorporation in products of food and pharmaceutical origin is
limited due to the presence of unwanted carbohydrates (mono- and disaccharide), and to
increase the functional value of the mixture, the attention was focused on their removal. In
this regard, membrane separation techniques present a very suitable solution for obtaining a
product with a higher FOS proportion. This study aimed to determine the optimal conditions
for purification process of produced FOS mixture using nanofiltration membrane modules.
Therefore, the influence of membranes with different MWCO (300-500Da and 600-800Da),
temperature (20-45°C) and carbohydrate concentration in feed solution (1-5%) at a constant
flow rate of 22 mL/min was investigated. From the initial mixture containing 60.2% FOS,
29.7% monosaccharides and 10.1% sucrose, after purification process under determined
optimal conditions (membrane 300-500Da, temperature 35°C and carbohydrate concentration
3%), the product with 88.8% FOS, 10.0% sucrose and 1.2% monosaccharides in total
carbohydrates was obtained. Purification yield was greater than 95% and the rejection
coefficients for glucose, fructose, sucrose, FOS3, FOS4 and FOS5 were 0.17, 0.20, 0.95,
0.99, 1.00 and 1.00, respectively. Based on the calculated purification factor, it can be
concluded that the initial mixture is refined 1.47 times. This result suggests a significant
increase in the purity of the final mixture, which can be used as a sugar substitute or addition
to numerous products that would be suitable for all consumers, including those suffering from
diabetes.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES
SP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6962
ER  - 

@conference{
author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Petrov Ivanković, Anja and Ćorović, Marija and Bezbradica, Dejan",
year = "2023",
abstract = "Fructo-oligosaccharides (FOS) are linear bioactive molecules consisting of one
terminal glucose and other fructose units and belong to the group of prebiotics with
scientifically proven benefits for human health. Physiological active compounds like
trisaccharides (FOS3), tetrasaccharides (FOS4) and pentasaccharides (FOS5) can be
produced from sucrose using enzyme-fructosyltransferase. The reaction mixture obtained in
this way contains, in addition to the desired prebiotics, glucose, fructose and an unreacted
amount of sucrose. Direct incorporation in products of food and pharmaceutical origin is
limited due to the presence of unwanted carbohydrates (mono- and disaccharide), and to
increase the functional value of the mixture, the attention was focused on their removal. In
this regard, membrane separation techniques present a very suitable solution for obtaining a
product with a higher FOS proportion. This study aimed to determine the optimal conditions
for purification process of produced FOS mixture using nanofiltration membrane modules.
Therefore, the influence of membranes with different MWCO (300-500Da and 600-800Da),
temperature (20-45°C) and carbohydrate concentration in feed solution (1-5%) at a constant
flow rate of 22 mL/min was investigated. From the initial mixture containing 60.2% FOS,
29.7% monosaccharides and 10.1% sucrose, after purification process under determined
optimal conditions (membrane 300-500Da, temperature 35°C and carbohydrate concentration
3%), the product with 88.8% FOS, 10.0% sucrose and 1.2% monosaccharides in total
carbohydrates was obtained. Purification yield was greater than 95% and the rejection
coefficients for glucose, fructose, sucrose, FOS3, FOS4 and FOS5 were 0.17, 0.20, 0.95,
0.99, 1.00 and 1.00, respectively. Based on the calculated purification factor, it can be
concluded that the initial mixture is refined 1.47 times. This result suggests a significant
increase in the purity of the final mixture, which can be used as a sugar substitute or addition
to numerous products that would be suitable for all consumers, including those suffering from
diabetes.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES",
pages = "86",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6962"
}

Veljković, M., Simović, M., Banjanac, K., Petrov Ivanković, A., Ćorović, M.,& Bezbradica, D.. (2023). NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 86.
https://hdl.handle.net/21.15107/rcub_technorep_6962

Veljković M, Simović M, Banjanac K, Petrov Ivanković A, Ćorović M, Bezbradica D. NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:86.
https://hdl.handle.net/21.15107/rcub_technorep_6962 .

Veljković, Milica, Simović, Milica, Banjanac, Katarina, Petrov Ivanković, Anja, Ćorović, Marija, Bezbradica, Dejan, "NANOFILTRATION AS A TOOL FOR HIGH-YIELD PURIFICATION OF DIETARY OLIGOSACCHARIDES" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):86,
https://hdl.handle.net/21.15107/rcub_technorep_6962 .

TY  - CONF
AU  - Ortega-Nieto, Clara
AU  - Losada-Gracia, Noelia
AU  - Vukoičić, Ana
AU  - Milivojević, Ana
AU  - Bezbradica, Dejan
AU  - Palomo, Jose M.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6959
AB  - In recent years, the synthesis of novel artificial metalloenzymes by the combination of
metal or complex organometallic systems and enzymes is rapidly growing field of research in
catalytic applications. In this study, new artificial metalloenzymes have been designed and
developed based on the combination of an enzyme and different metal salts. For this purpose,
novel synthesis method has been used, where the enzyme, Lipase B from Candida antarctica
(CALB), acts as an inducer of metal nanoparticles of copper and silver. Obtained biohybrids
show improved stability properties compared to natural enzymes. Moreover, the synergetic
effect between copper and silver has been evaluated, looking for an improvement in the
catalytic efficiency of an only copper-metalloenzyme previously developed.
The copper-silver-CALB metalloenzymes have been characterized by X-Ray
diffraction analysis, mass spectrometry, and SEM and TEM as microscopies techniques. After
that, their catalytic efficiency was tested in different reactions, in particular in the phloridzin
oligomerization as laccase-like activity.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6959
ER  - 

@conference{
author = "Ortega-Nieto, Clara and Losada-Gracia, Noelia and Vukoičić, Ana and Milivojević, Ana and Bezbradica, Dejan and Palomo, Jose M.",
year = "2023",
abstract = "In recent years, the synthesis of novel artificial metalloenzymes by the combination of
metal or complex organometallic systems and enzymes is rapidly growing field of research in
catalytic applications. In this study, new artificial metalloenzymes have been designed and
developed based on the combination of an enzyme and different metal salts. For this purpose,
novel synthesis method has been used, where the enzyme, Lipase B from Candida antarctica
(CALB), acts as an inducer of metal nanoparticles of copper and silver. Obtained biohybrids
show improved stability properties compared to natural enzymes. Moreover, the synergetic
effect between copper and silver has been evaluated, looking for an improvement in the
catalytic efficiency of an only copper-metalloenzyme previously developed.
The copper-silver-CALB metalloenzymes have been characterized by X-Ray
diffraction analysis, mass spectrometry, and SEM and TEM as microscopies techniques. After
that, their catalytic efficiency was tested in different reactions, in particular in the phloridzin
oligomerization as laccase-like activity.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6959"
}

Ortega-Nieto, C., Losada-Gracia, N., Vukoičić, A., Milivojević, A., Bezbradica, D.,& Palomo, J. M.. (2023). NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 39.
https://hdl.handle.net/21.15107/rcub_technorep_6959

Ortega-Nieto C, Losada-Gracia N, Vukoičić A, Milivojević A, Bezbradica D, Palomo JM. NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:39.
https://hdl.handle.net/21.15107/rcub_technorep_6959 .

Ortega-Nieto, Clara, Losada-Gracia, Noelia, Vukoičić, Ana, Milivojević, Ana, Bezbradica, Dejan, Palomo, Jose M., "NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):39,
https://hdl.handle.net/21.15107/rcub_technorep_6959 .

TY  - CONF
AU  - García-Sanz, Carla
AU  - Andreu, Alicia
AU  - de las Rivas, Blanca
AU  - Muñoz, Rosario
AU  - Vukoičić, Ana
AU  - Milivojević, Ana
AU  - Bezbradica, Dejan
AU  - Palomo, Jose Miguel
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6949
AB  - Laccase (EC 1.10.3.2, p-diphenol: dioxygen oxidoreductase) is a blue copper oxidase
of great industrial interest due to its ability to catalyseoxidation processes of phenols and
persistent organic pollutants. However, it is susceptible to denaturation by high temperatures,
and sensitive to pH and the presence of high concentrations of solvents, which is a problem
for industrial use. To solve this problem, this project develops the synthesis in aqueous
medium of a new Mn metalloenzyme with laccase oxidase mimetic catalytic activity. To do
this, Geobacillus thermocatenulatus lipase (GTL) is used as a "scaffold" enzyme, which is
mixed with a manganese salt at 50 ºC in an aqueous medium. In this way, the in situ
formation of manganese (IV) oxide nanowires is generated, interacting with the enzyme and
obtaining the GTL-Mn bioconjugate. On the other hand, its oxidative activity was evaluated
using the ABTS assay, obtaining a specificity 300 times greater than the laccase from
Trametes versicolor and 2 times more than the laccase from Myceliophthora thermophila
expressed in Aspergillus oryzae (Novozym 51003®). In addition, the new metalloenzyme
turned out to be 2 times more stable at 40 ºC, 3 times more stable in thepresence of 10% can,
and 10 times more stable at 20% and 30% AcN than laccase (Novozym 51003®) by
evaluating it at 2 hour incubation. Moreover, it was shown that the use of immobilization
strategies improves the stabilization of this artificial metalloenzyme two times more. Finally,
the novel Mn metalloenzymes were effective in the reaction of phlorizin oligomerization.
Future research will aim to extend this study for the use of flavonoids as prebiotic molecules
in the cosmetics industry.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - STUDY AND PREPARATION OF ARTIFICIAL MANGANESE METALLOENZYMES WITH LACCASE LIKE ACTIVITY
SP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6949
ER  - 

@conference{
author = "García-Sanz, Carla and Andreu, Alicia and de las Rivas, Blanca and Muñoz, Rosario and Vukoičić, Ana and Milivojević, Ana and Bezbradica, Dejan and Palomo, Jose Miguel",
year = "2023",
abstract = "Laccase (EC 1.10.3.2, p-diphenol: dioxygen oxidoreductase) is a blue copper oxidase
of great industrial interest due to its ability to catalyseoxidation processes of phenols and
persistent organic pollutants. However, it is susceptible to denaturation by high temperatures,
and sensitive to pH and the presence of high concentrations of solvents, which is a problem
for industrial use. To solve this problem, this project develops the synthesis in aqueous
medium of a new Mn metalloenzyme with laccase oxidase mimetic catalytic activity. To do
this, Geobacillus thermocatenulatus lipase (GTL) is used as a "scaffold" enzyme, which is
mixed with a manganese salt at 50 ºC in an aqueous medium. In this way, the in situ
formation of manganese (IV) oxide nanowires is generated, interacting with the enzyme and
obtaining the GTL-Mn bioconjugate. On the other hand, its oxidative activity was evaluated
using the ABTS assay, obtaining a specificity 300 times greater than the laccase from
Trametes versicolor and 2 times more than the laccase from Myceliophthora thermophila
expressed in Aspergillus oryzae (Novozym 51003®). In addition, the new metalloenzyme
turned out to be 2 times more stable at 40 ºC, 3 times more stable in thepresence of 10% can,
and 10 times more stable at 20% and 30% AcN than laccase (Novozym 51003®) by
evaluating it at 2 hour incubation. Moreover, it was shown that the use of immobilization
strategies improves the stabilization of this artificial metalloenzyme two times more. Finally,
the novel Mn metalloenzymes were effective in the reaction of phlorizin oligomerization.
Future research will aim to extend this study for the use of flavonoids as prebiotic molecules
in the cosmetics industry.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "STUDY AND PREPARATION OF ARTIFICIAL MANGANESE METALLOENZYMES WITH LACCASE LIKE ACTIVITY",
pages = "35",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6949"
}

García-Sanz, C., Andreu, A., de las Rivas, B., Muñoz, R., Vukoičić, A., Milivojević, A., Bezbradica, D.,& Palomo, J. M.. (2023). STUDY AND PREPARATION OF ARTIFICIAL MANGANESE METALLOENZYMES WITH LACCASE LIKE ACTIVITY. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 35.
https://hdl.handle.net/21.15107/rcub_technorep_6949

García-Sanz C, Andreu A, de las Rivas B, Muñoz R, Vukoičić A, Milivojević A, Bezbradica D, Palomo JM. STUDY AND PREPARATION OF ARTIFICIAL MANGANESE METALLOENZYMES WITH LACCASE LIKE ACTIVITY. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:35.
https://hdl.handle.net/21.15107/rcub_technorep_6949 .

García-Sanz, Carla, Andreu, Alicia, de las Rivas, Blanca, Muñoz, Rosario, Vukoičić, Ana, Milivojević, Ana, Bezbradica, Dejan, Palomo, Jose Miguel, "STUDY AND PREPARATION OF ARTIFICIAL MANGANESE METALLOENZYMES WITH LACCASE LIKE ACTIVITY" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):35,
https://hdl.handle.net/21.15107/rcub_technorep_6949 .

TY  - CONF
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Vukoičić, Ana
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6843
AB  - With the increasing interest in functional products, prebiotics that selectively stimulate growth and activity of good bacteria in human gut, gained attention from both general and scientific community. The range of food products containing prebiotic oligosaccharides is rapidly expanding, causing their intensified production. Currently, there are a few recognized prebiotics, amongst which, fructo-oligosaccharides (FOS) proved to be particularly interesting owing to the confirmed positive effect on human wellbeing (restoring microbiota balance, improving immunity and lipid metabolism, enhancing vitamin production and calcium absorption) and exceptional physicochemical qualities. Namely, good taste quality, low sweetness, and low caloric value make them highly applicable supplement in food industry. Fructo-oligosaccharides are scarcely present in natural sources and nowadays are mainly produced by enzymatic conversion of sucrose and sucrose-rich substrates. Bearing in mind the complexity of synthesis process, resulting reaction mixture besides FOS (mixture of tri-, tetra- and pentasaccharides) contains significant portion of sucrose and monosaccharides. Within this study, efficient method for obtaining high-purity product upon sucrose conversion using fructosyltransferase (Pectinex® Ultra SP-L) was developed through detailed optimization of membrane separation processes. Under determined optimal conditions for nanofiltration (temperature of 35 °C, concentration of initial sugars of 3% and flow rate of 25 mL/min), undesired components (monosaccharides and partially sucrose) were removed from the initial mixture (58% FOS in total carbohydrates) and 1.48-fold increase the proportion of targeted bioactive components was achieved. Thus, product of high purity (86% FOS in total carbohydrates) was obtained with remarkable recovery yields of 90%. The prebiotic activity of the obtained product was confirmed in in vitro tests with several microbial cultures comprising representatives of beneficial (genera Lactobacillus and Saccharomyces) and pathogenic (Escherichia coli) gut microbiota. Having said that, it can be concluded that high-purity prebiotic product with great potential for application in different value-added food industry products was obtained.
PB  - Belgrade : University, Faculty of Geography
C3  - Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023
T1  - Effective approach for obtaining high-purity prebiotics using membrane separation technology
SP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6843
ER  - 

@conference{
author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Milivojević, Ana and Ćorović, Marija and Vukoičić, Ana and Bezbradica, Dejan",
year = "2023",
abstract = "With the increasing interest in functional products, prebiotics that selectively stimulate growth and activity of good bacteria in human gut, gained attention from both general and scientific community. The range of food products containing prebiotic oligosaccharides is rapidly expanding, causing their intensified production. Currently, there are a few recognized prebiotics, amongst which, fructo-oligosaccharides (FOS) proved to be particularly interesting owing to the confirmed positive effect on human wellbeing (restoring microbiota balance, improving immunity and lipid metabolism, enhancing vitamin production and calcium absorption) and exceptional physicochemical qualities. Namely, good taste quality, low sweetness, and low caloric value make them highly applicable supplement in food industry. Fructo-oligosaccharides are scarcely present in natural sources and nowadays are mainly produced by enzymatic conversion of sucrose and sucrose-rich substrates. Bearing in mind the complexity of synthesis process, resulting reaction mixture besides FOS (mixture of tri-, tetra- and pentasaccharides) contains significant portion of sucrose and monosaccharides. Within this study, efficient method for obtaining high-purity product upon sucrose conversion using fructosyltransferase (Pectinex® Ultra SP-L) was developed through detailed optimization of membrane separation processes. Under determined optimal conditions for nanofiltration (temperature of 35 °C, concentration of initial sugars of 3% and flow rate of 25 mL/min), undesired components (monosaccharides and partially sucrose) were removed from the initial mixture (58% FOS in total carbohydrates) and 1.48-fold increase the proportion of targeted bioactive components was achieved. Thus, product of high purity (86% FOS in total carbohydrates) was obtained with remarkable recovery yields of 90%. The prebiotic activity of the obtained product was confirmed in in vitro tests with several microbial cultures comprising representatives of beneficial (genera Lactobacillus and Saccharomyces) and pathogenic (Escherichia coli) gut microbiota. Having said that, it can be concluded that high-purity prebiotic product with great potential for application in different value-added food industry products was obtained.",
publisher = "Belgrade : University, Faculty of Geography",
journal = "Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023",
title = "Effective approach for obtaining high-purity prebiotics using membrane separation technology",
pages = "25",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6843"
}

Veljković, M., Simović, M., Banjanac, K., Milivojević, A., Ćorović, M., Vukoičić, A.,& Bezbradica, D.. (2023). Effective approach for obtaining high-purity prebiotics using membrane separation technology. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023
Belgrade : University, Faculty of Geography., 25.
https://hdl.handle.net/21.15107/rcub_technorep_6843

Veljković M, Simović M, Banjanac K, Milivojević A, Ćorović M, Vukoičić A, Bezbradica D. Effective approach for obtaining high-purity prebiotics using membrane separation technology. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023. 2023;:25.
https://hdl.handle.net/21.15107/rcub_technorep_6843 .

Veljković, Milica, Simović, Milica, Banjanac, Katarina, Milivojević, Ana, Ćorović, Marija, Vukoičić, Ana, Bezbradica, Dejan, "Effective approach for obtaining high-purity prebiotics using membrane separation technology" in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023 (2023):25,
https://hdl.handle.net/21.15107/rcub_technorep_6843 .

TY  - CONF
AU  - Vukoičić, Ana
AU  - Milivojević, Ana
AU  - Baščarević, Jana
AU  - Petrov Ivanković, Anja
AU  - Banjanac, Katarina
AU  - Ćorović, Marija
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6945
AB  - Dihydrochalcones are a class of flavonoids found in apple trees (Malus sp) generally
present in high levels in young leaves and immature fruits. Phloridzin is a member of the
dihydrochalcones which possess good antioxidant activity, as well as a protective role against
UV radiation, hence it is a constituent of various commercial cosmetic preparations. These
natural low-molecular weight phenolic compounds with diverse physicochemical
characteristics are substrates for laccases, copper-containing polyphenol oxidases whose
activity can yield compounds that vary in structure and biological activities. The positive
effect of laccase-catalyzed polymerization of flavonoids on the functionality of produced
oligomers or polymers has been already shown on some flavonoids’ representatives.
In this work, fungal laccase from Trametes versicolor was applied as a biocatalyst in
the reaction of phloridzin oligomerization. Synthesized phloridzin oligomers were separated
from the reaction mixture and lyophilized in order to test their antioxidant and antimicrobial
activity. Different antioxidant assays, such as ABTS (2,2’-azino-bis(3-
ethylbenzenothiazoline-6-sulfonic acid) diammonium salt) and DPPH (2,2-diphenyl-1-
picrylhydrazyl) showed that in comparison with the monomer, synthesized oligomers had the
weaker ability to scavenge the free ABTS•+ and DPPH•
radicals, since for both assays,
examined IC50 values of phloridzin oligomers (0.46 mg/ml and 1.52 mg/ml, respectively)
was higher than the values for parent molecule (0.13 mg/ml and 1.07 mg/ml, respectively).
This could be ascribed to the fact that some of the functional groups responsible for the
antioxidant activity probably participate in the formation of linkage between phloridzin units
in the structure of oligomers. On the other hand, synthesized phloridzin oligomers
demonstrated a significantly higher antimicrobial effect on the Gram-positive
bacteria Staphylococcus aureus compared to the parent molecule phloridzin. Moreover, the
greater inhibition of bacterial growth was achieved by increasing the oligomers’
concentration. Given the obtained results, synthesized compounds show potential to be
further examined as dermal prebiotics.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - EVALUATION OF BIOLOGICAL ACTIVITY OF ENZYMATICALLY SYNTHESIZED PHLORIDZIN OLIGOMERS
SP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6945
ER  - 

@conference{
author = "Vukoičić, Ana and Milivojević, Ana and Baščarević, Jana and Petrov Ivanković, Anja and Banjanac, Katarina and Ćorović, Marija and Bezbradica, Dejan",
year = "2023",
abstract = "Dihydrochalcones are a class of flavonoids found in apple trees (Malus sp) generally
present in high levels in young leaves and immature fruits. Phloridzin is a member of the
dihydrochalcones which possess good antioxidant activity, as well as a protective role against
UV radiation, hence it is a constituent of various commercial cosmetic preparations. These
natural low-molecular weight phenolic compounds with diverse physicochemical
characteristics are substrates for laccases, copper-containing polyphenol oxidases whose
activity can yield compounds that vary in structure and biological activities. The positive
effect of laccase-catalyzed polymerization of flavonoids on the functionality of produced
oligomers or polymers has been already shown on some flavonoids’ representatives.
In this work, fungal laccase from Trametes versicolor was applied as a biocatalyst in
the reaction of phloridzin oligomerization. Synthesized phloridzin oligomers were separated
from the reaction mixture and lyophilized in order to test their antioxidant and antimicrobial
activity. Different antioxidant assays, such as ABTS (2,2’-azino-bis(3-
ethylbenzenothiazoline-6-sulfonic acid) diammonium salt) and DPPH (2,2-diphenyl-1-
picrylhydrazyl) showed that in comparison with the monomer, synthesized oligomers had the
weaker ability to scavenge the free ABTS•+ and DPPH•
radicals, since for both assays,
examined IC50 values of phloridzin oligomers (0.46 mg/ml and 1.52 mg/ml, respectively)
was higher than the values for parent molecule (0.13 mg/ml and 1.07 mg/ml, respectively).
This could be ascribed to the fact that some of the functional groups responsible for the
antioxidant activity probably participate in the formation of linkage between phloridzin units
in the structure of oligomers. On the other hand, synthesized phloridzin oligomers
demonstrated a significantly higher antimicrobial effect on the Gram-positive
bacteria Staphylococcus aureus compared to the parent molecule phloridzin. Moreover, the
greater inhibition of bacterial growth was achieved by increasing the oligomers’
concentration. Given the obtained results, synthesized compounds show potential to be
further examined as dermal prebiotics.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "EVALUATION OF BIOLOGICAL ACTIVITY OF ENZYMATICALLY SYNTHESIZED PHLORIDZIN OLIGOMERS",
pages = "26",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6945"
}

Vukoičić, A., Milivojević, A., Baščarević, J., Petrov Ivanković, A., Banjanac, K., Ćorović, M.,& Bezbradica, D.. (2023). EVALUATION OF BIOLOGICAL ACTIVITY OF ENZYMATICALLY SYNTHESIZED PHLORIDZIN OLIGOMERS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 26.
https://hdl.handle.net/21.15107/rcub_technorep_6945

Vukoičić A, Milivojević A, Baščarević J, Petrov Ivanković A, Banjanac K, Ćorović M, Bezbradica D. EVALUATION OF BIOLOGICAL ACTIVITY OF ENZYMATICALLY SYNTHESIZED PHLORIDZIN OLIGOMERS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:26.
https://hdl.handle.net/21.15107/rcub_technorep_6945 .

Vukoičić, Ana, Milivojević, Ana, Baščarević, Jana, Petrov Ivanković, Anja, Banjanac, Katarina, Ćorović, Marija, Bezbradica, Dejan, "EVALUATION OF BIOLOGICAL ACTIVITY OF ENZYMATICALLY SYNTHESIZED PHLORIDZIN OLIGOMERS" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):26,
https://hdl.handle.net/21.15107/rcub_technorep_6945 .

TY  - CONF
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Veljković, Milica
AU  - Vukoičić, Ana
AU  - Simović, Milica
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6946
AB  - Berries are known to be one of the richest sources of polyphenols which can offer
various health benefits. Dietary supplementation with berries has a positive influence on the
gut microbiota, which directly affects overall health, including skin health. However, topical
application of berry polyphenols has been used mainly for its antioxidant activity to prevent
premature aging and improve the skin's appearance. Therefore, this study examined the
content of different polyphenol classes of cranberry (Vaccinium macrocarpa), chokeberry
(Aronia melanocarpa) and blueberry (Vaccinium angustifolium) extracts, investigating their
antioxidant properties and potential impact on skin as topical prebiotics. The prebiotic
capacity of these extracts in applied concentrations range of 0.015-0.05 mg GAE/mL, was
determined against two cutaneous bacteria - beneficial Staphylococcus epidermidis and
opportunistic pathogen Staphylococcus aureus, since the disrupted balance between them
may contribute worsening of atopic dermatitis. The obtained results showed that the total
polyphenol content was highest in chokeberry extract (9.88 mg GAE/g DM), followed by
cranberry extract (8.78 mg GAE/g DM), and the lowest in blueberry extract (6.48 mg GAE/g
DM). Chokeberry extract was also richest in flavonoids, flavonols, anthocyanins and phenolic
acids. Notably, cranberry extract had the highest concentration of tannins, almost three times
higher compared to blueberry extract. According to DPPH and ABTS methods, the
antioxidant activity was significantly high in chokeberry extract, while FRAP method
revealed that cranberry extract is the most potent antioxidant. Regarding prebiotic capacity,
positive values (0.10-0.48) were observed at all concentrations of cranberry extract, with a
trend indicating a decrease in prebiotic capacity as polyphenol concentration increases.
Prebiotic capacities of blueberry and chokeberry extracts had either negative values or values
equal to zero, indicating that these extracts do not positively impact the microorganisms’
ratio. Obtained findings suggest cranberry extract potential for enhancing both antioxidant
defense of skin and rebalancing cutaneous microbiota.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS
SP  - 29
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6946
ER  - 

@conference{
author = "Petrov Ivanković, Anja and Ćorović, Marija and Milivojević, Ana and Veljković, Milica and Vukoičić, Ana and Simović, Milica and Bezbradica, Dejan",
year = "2023",
abstract = "Berries are known to be one of the richest sources of polyphenols which can offer
various health benefits. Dietary supplementation with berries has a positive influence on the
gut microbiota, which directly affects overall health, including skin health. However, topical
application of berry polyphenols has been used mainly for its antioxidant activity to prevent
premature aging and improve the skin's appearance. Therefore, this study examined the
content of different polyphenol classes of cranberry (Vaccinium macrocarpa), chokeberry
(Aronia melanocarpa) and blueberry (Vaccinium angustifolium) extracts, investigating their
antioxidant properties and potential impact on skin as topical prebiotics. The prebiotic
capacity of these extracts in applied concentrations range of 0.015-0.05 mg GAE/mL, was
determined against two cutaneous bacteria - beneficial Staphylococcus epidermidis and
opportunistic pathogen Staphylococcus aureus, since the disrupted balance between them
may contribute worsening of atopic dermatitis. The obtained results showed that the total
polyphenol content was highest in chokeberry extract (9.88 mg GAE/g DM), followed by
cranberry extract (8.78 mg GAE/g DM), and the lowest in blueberry extract (6.48 mg GAE/g
DM). Chokeberry extract was also richest in flavonoids, flavonols, anthocyanins and phenolic
acids. Notably, cranberry extract had the highest concentration of tannins, almost three times
higher compared to blueberry extract. According to DPPH and ABTS methods, the
antioxidant activity was significantly high in chokeberry extract, while FRAP method
revealed that cranberry extract is the most potent antioxidant. Regarding prebiotic capacity,
positive values (0.10-0.48) were observed at all concentrations of cranberry extract, with a
trend indicating a decrease in prebiotic capacity as polyphenol concentration increases.
Prebiotic capacities of blueberry and chokeberry extracts had either negative values or values
equal to zero, indicating that these extracts do not positively impact the microorganisms’
ratio. Obtained findings suggest cranberry extract potential for enhancing both antioxidant
defense of skin and rebalancing cutaneous microbiota.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS",
pages = "29",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6946"
}

Petrov Ivanković, A., Ćorović, M., Milivojević, A., Veljković, M., Vukoičić, A., Simović, M.,& Bezbradica, D.. (2023). DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 29.
https://hdl.handle.net/21.15107/rcub_technorep_6946

Petrov Ivanković A, Ćorović M, Milivojević A, Veljković M, Vukoičić A, Simović M, Bezbradica D. DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:29.
https://hdl.handle.net/21.15107/rcub_technorep_6946 .

Petrov Ivanković, Anja, Ćorović, Marija, Milivojević, Ana, Veljković, Milica, Vukoičić, Ana, Simović, Milica, Bezbradica, Dejan, "DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):29,
https://hdl.handle.net/21.15107/rcub_technorep_6946 .

TY  - CONF
AU  - dos Santos, Leandro Alves
AU  - Banjanac, Katarina
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Mateo, Cesar
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6948
AB  - A food-grade bacterial xylanase enzyme preparation, ROHALASE® SEP-VISCO,
was immobilized by adsorption onto polyethyleneimine (PEI) functionalized magnetic
nanoparticles (MNPs). This process resulted in nanobiocatalysts with optimal characteristics.
Subsequently, this nanobiocatalyst was employed to produce xylo-oligosaccharides (XOS). In
the past decade, magnetic nanoparticles (MNPs) have gained significant interest, finding
diverse applications in biomedicine, biosensor production, food processing, catalysis,
agriculture, and environmental processes. MNPs have proven highly effective for enzyme
immobilization because of their unique and distinguished properties such as high specific
surface area, biocompatibility, and magnetic characteristics. Magnetic characteristics
facilitate the easy removal of MNPs from reaction mixtures, allowing for their efficient reuse.
The functionalization of MNPs through with the cationic polymer PEI can further augment
their ability for enzyme attachment. Generally, xylanases hydrolyses β-1,4-glycosidic
linkages present in xylan which is a major component of the hemicellulosic fraction in the
plant cell walls. Furthermore, xylanases can produce XOS composed of xylose units linked
by β-1,4-xylosidic bonds. XOS have stimulatory effects on the selective growth of human
intestinal microbiota and are frequently defined as prebiotics.
In the present work, the xylanase immobilization efficiency on MNPs-PEI is between
100 and 43 % within the wide range of xylanase concentrations (400-2400 mg/g of support).
Free and immobilized xylanase showed maximal catalytic activity at pH 6.0 and 60°C in
reaction with commercial birchwood xylan (concentration of 1 % w/v). The maximum
activity of 1675 IU/g of support was achieved when immobilization was performed at initial
enzyme concentration of 1250 mg/g of support during 2 h. Since this immobilized
preparation exhibited the activity immobilization yield of 80 % and specific activity of 2.1
mg of proteins/g of support, it has been applied in reaction of XOS synthesis. The MNPsPEI-xylanase was found to produce high yield of XOS from birchwood xylan, indicating its
potential for utilization in feed and food formulations.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6948
ER  - 

@conference{
author = "dos Santos, Leandro Alves and Banjanac, Katarina and Veljković, Milica and Simović, Milica and Mateo, Cesar and Bezbradica, Dejan",
year = "2023",
abstract = "A food-grade bacterial xylanase enzyme preparation, ROHALASE® SEP-VISCO,
was immobilized by adsorption onto polyethyleneimine (PEI) functionalized magnetic
nanoparticles (MNPs). This process resulted in nanobiocatalysts with optimal characteristics.
Subsequently, this nanobiocatalyst was employed to produce xylo-oligosaccharides (XOS). In
the past decade, magnetic nanoparticles (MNPs) have gained significant interest, finding
diverse applications in biomedicine, biosensor production, food processing, catalysis,
agriculture, and environmental processes. MNPs have proven highly effective for enzyme
immobilization because of their unique and distinguished properties such as high specific
surface area, biocompatibility, and magnetic characteristics. Magnetic characteristics
facilitate the easy removal of MNPs from reaction mixtures, allowing for their efficient reuse.
The functionalization of MNPs through with the cationic polymer PEI can further augment
their ability for enzyme attachment. Generally, xylanases hydrolyses β-1,4-glycosidic
linkages present in xylan which is a major component of the hemicellulosic fraction in the
plant cell walls. Furthermore, xylanases can produce XOS composed of xylose units linked
by β-1,4-xylosidic bonds. XOS have stimulatory effects on the selective growth of human
intestinal microbiota and are frequently defined as prebiotics.
In the present work, the xylanase immobilization efficiency on MNPs-PEI is between
100 and 43 % within the wide range of xylanase concentrations (400-2400 mg/g of support).
Free and immobilized xylanase showed maximal catalytic activity at pH 6.0 and 60°C in
reaction with commercial birchwood xylan (concentration of 1 % w/v). The maximum
activity of 1675 IU/g of support was achieved when immobilization was performed at initial
enzyme concentration of 1250 mg/g of support during 2 h. Since this immobilized
preparation exhibited the activity immobilization yield of 80 % and specific activity of 2.1
mg of proteins/g of support, it has been applied in reaction of XOS synthesis. The MNPsPEI-xylanase was found to produce high yield of XOS from birchwood xylan, indicating its
potential for utilization in feed and food formulations.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS",
pages = "34",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6948"
}

dos Santos, L. A., Banjanac, K., Veljković, M., Simović, M., Mateo, C.,& Bezbradica, D.. (2023). IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 34.
https://hdl.handle.net/21.15107/rcub_technorep_6948

dos Santos LA, Banjanac K, Veljković M, Simović M, Mateo C, Bezbradica D. IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:34.
https://hdl.handle.net/21.15107/rcub_technorep_6948 .

dos Santos, Leandro Alves, Banjanac, Katarina, Veljković, Milica, Simović, Milica, Mateo, Cesar, Bezbradica, Dejan, "IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):34,
https://hdl.handle.net/21.15107/rcub_technorep_6948 .

TY  - CONF
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Blagojević, Stevan
AU  - Radulović, Aleksandra
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6947
AB  - Blackcurrant is anthocyanin-rich berry with proven antioxidant and photoprotective
activity and emerging prebiotic potential, widely applied in cosmetic products. Although
beneficial effects of its extract are well known, release from skin care products and
(trans)dermal permeation were not previously investigated.
In this study, enzyme-assisted extraction of polyphenols from lyophilized
blackcurrant was performed and obtained extract was incorporated into an Aristoflex® AVC
based hydrogel. Furthermore, Franz diffusion cell experiments were conducted in order to
examine the release of these molecules from the prepared formulation using cellulose acetate
membrane and transdermal and dermal delivery using human skin mimicking membrane
(Strat® M).
Obtained results revealed that all four dominant anthocyanins readily permeated from
hydrogel since 17.5%, 32.8%, 33.8% and 39.2% of delphinidin 3-glucoside, delphinidin 3-
rutinoside, cyanidin 3-glucoside and cyanidin 3-rutinoside, respectively, were detected in
receptor fluid after 24 h. Experimental values were successfully fitted with the Peppas and
Sahlin diffusion model. On the other hand, after 72 h long experiment with transdermal skin
diffusion model (Strat® M membrane), no detectable amounts of anthocyanins were present
in receptor fluid and only 0.5% of the initial quantity from donor compartment was extracted
from the membrane itself, indicating weak dermal delivery.
Present study revealed that hydrogel is suitable carrier system for the topical delivery
of bioactive anthocyanins from blackcurrant, while dermal and transdermal delivery of these
molecules is very limited. This implies applicability of blackcurrant extract for treatments
targeting skin surface (i.e. antioxidant, prebiotic, photoprotective).
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - IN VITRO RELEASE AND (TRANS)DERMAL DELIVERY STUDY OF BLACKCURRANT ANTHOCYANINS INCORPORATED IN COSMETIC FORMULATION
SP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6947
ER  - 

@conference{
author = "Petrov Ivanković, Anja and Ćorović, Marija and Milivojević, Ana and Blagojević, Stevan and Radulović, Aleksandra and Pjanović, Rada and Bezbradica, Dejan",
year = "2023",
abstract = "Blackcurrant is anthocyanin-rich berry with proven antioxidant and photoprotective
activity and emerging prebiotic potential, widely applied in cosmetic products. Although
beneficial effects of its extract are well known, release from skin care products and
(trans)dermal permeation were not previously investigated.
In this study, enzyme-assisted extraction of polyphenols from lyophilized
blackcurrant was performed and obtained extract was incorporated into an Aristoflex® AVC
based hydrogel. Furthermore, Franz diffusion cell experiments were conducted in order to
examine the release of these molecules from the prepared formulation using cellulose acetate
membrane and transdermal and dermal delivery using human skin mimicking membrane
(Strat® M).
Obtained results revealed that all four dominant anthocyanins readily permeated from
hydrogel since 17.5%, 32.8%, 33.8% and 39.2% of delphinidin 3-glucoside, delphinidin 3-
rutinoside, cyanidin 3-glucoside and cyanidin 3-rutinoside, respectively, were detected in
receptor fluid after 24 h. Experimental values were successfully fitted with the Peppas and
Sahlin diffusion model. On the other hand, after 72 h long experiment with transdermal skin
diffusion model (Strat® M membrane), no detectable amounts of anthocyanins were present
in receptor fluid and only 0.5% of the initial quantity from donor compartment was extracted
from the membrane itself, indicating weak dermal delivery.
Present study revealed that hydrogel is suitable carrier system for the topical delivery
of bioactive anthocyanins from blackcurrant, while dermal and transdermal delivery of these
molecules is very limited. This implies applicability of blackcurrant extract for treatments
targeting skin surface (i.e. antioxidant, prebiotic, photoprotective).",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "IN VITRO RELEASE AND (TRANS)DERMAL DELIVERY STUDY OF BLACKCURRANT ANTHOCYANINS INCORPORATED IN COSMETIC FORMULATION",
pages = "30",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6947"
}

Petrov Ivanković, A., Ćorović, M., Milivojević, A., Blagojević, S., Radulović, A., Pjanović, R.,& Bezbradica, D.. (2023). IN VITRO RELEASE AND (TRANS)DERMAL DELIVERY STUDY OF BLACKCURRANT ANTHOCYANINS INCORPORATED IN COSMETIC FORMULATION. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 30.
https://hdl.handle.net/21.15107/rcub_technorep_6947

Petrov Ivanković A, Ćorović M, Milivojević A, Blagojević S, Radulović A, Pjanović R, Bezbradica D. IN VITRO RELEASE AND (TRANS)DERMAL DELIVERY STUDY OF BLACKCURRANT ANTHOCYANINS INCORPORATED IN COSMETIC FORMULATION. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:30.
https://hdl.handle.net/21.15107/rcub_technorep_6947 .

Petrov Ivanković, Anja, Ćorović, Marija, Milivojević, Ana, Blagojević, Stevan, Radulović, Aleksandra, Pjanović, Rada, Bezbradica, Dejan, "IN VITRO RELEASE AND (TRANS)DERMAL DELIVERY STUDY OF BLACKCURRANT ANTHOCYANINS INCORPORATED IN COSMETIC FORMULATION" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):30,
https://hdl.handle.net/21.15107/rcub_technorep_6947 .

TY  - CONF
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Jaćimovski, Darko
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6871
AB  - Scientific breakthroughs in research of human microbiota revealed that significant number of
diseases related to modern food intake and sedentary lifestyles are caused by dysbiosis of human
microbiota composition, hence substantial increase in interest for products helping restore this balance
(such as probiotics and prebiotics) within food, cosmetic and health industry was observed. In recent
times, prebiotics have been recognized as attractive products due to their higher stability to processing
and storage conditions, lower susceptibility to interaction with other compounds in more complex
formulation and higher reproducibility of their physiological effects [1]. Consequential rapid growth of
market demand brought challenges for biochemical industry, such as requirements to increase
production capacity and decrease costs by development of more efficient enzymatic processes and
product separation.
Majority of prebiotic compounds are classified as oligosaccharides, which can be obtained
either by enzymatic synthesis from compounds of lower degree of polymerization (DP) - for example:
galacto-oligosaccharides (GOS) from lactose and fructo-oligosaccharides (FOS) from sucrose - or by
enzymatic hydrolysis, for example pectic oligosaccharides (POS) from pectin or xylo-oligosaccharides
(XOS) from xylan [2]. In both cases, membrane separation is efficient tool for product purification,
because OS obtained by synthesis can be separated from lower DP compounds using nanofiltrations,
while those obtained by hydrolysis can be separated from compounds of higher DP using ultrafiltration.
Hence, it can be expected that selection of adequate membrane material, pore dimensions and
downstream processing conditions will have important role in development of novel prebiotic
technologies.
In this study nanofiltration membrane module was applied with a purpose of purification of
FOS obtained in enzymatic synthesis using sucrose as substrate. Two strategies were applied:
• purification of reaction mixture obtained after enzymatic synthesis,
• simultaneous enzymatic synthesis and purification in nanofiltration membrane module.
PB  - TECNALIA
C3  - Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain
T1  - Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module
EP  - 85
EP  - 
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6871
ER  - 

@conference{
author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Milivojević, Ana and Ćorović, Marija and Jaćimovski, Darko and Bezbradica, Dejan",
year = "2023",
abstract = "Scientific breakthroughs in research of human microbiota revealed that significant number of
diseases related to modern food intake and sedentary lifestyles are caused by dysbiosis of human
microbiota composition, hence substantial increase in interest for products helping restore this balance
(such as probiotics and prebiotics) within food, cosmetic and health industry was observed. In recent
times, prebiotics have been recognized as attractive products due to their higher stability to processing
and storage conditions, lower susceptibility to interaction with other compounds in more complex
formulation and higher reproducibility of their physiological effects [1]. Consequential rapid growth of
market demand brought challenges for biochemical industry, such as requirements to increase
production capacity and decrease costs by development of more efficient enzymatic processes and
product separation.
Majority of prebiotic compounds are classified as oligosaccharides, which can be obtained
either by enzymatic synthesis from compounds of lower degree of polymerization (DP) - for example:
galacto-oligosaccharides (GOS) from lactose and fructo-oligosaccharides (FOS) from sucrose - or by
enzymatic hydrolysis, for example pectic oligosaccharides (POS) from pectin or xylo-oligosaccharides
(XOS) from xylan [2]. In both cases, membrane separation is efficient tool for product purification,
because OS obtained by synthesis can be separated from lower DP compounds using nanofiltrations,
while those obtained by hydrolysis can be separated from compounds of higher DP using ultrafiltration.
Hence, it can be expected that selection of adequate membrane material, pore dimensions and
downstream processing conditions will have important role in development of novel prebiotic
technologies.
In this study nanofiltration membrane module was applied with a purpose of purification of
FOS obtained in enzymatic synthesis using sucrose as substrate. Two strategies were applied:
• purification of reaction mixture obtained after enzymatic synthesis,
• simultaneous enzymatic synthesis and purification in nanofiltration membrane module.",
publisher = "TECNALIA",
journal = "Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain",
title = "Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module",
pages = "85--84",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6871"
}

Veljković, M., Simović, M., Banjanac, K., Milivojević, A., Ćorović, M., Jaćimovski, D.,& Bezbradica, D.. (2023). Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module. in Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain
TECNALIA., 84-85.
https://hdl.handle.net/21.15107/rcub_technorep_6871

Veljković M, Simović M, Banjanac K, Milivojević A, Ćorović M, Jaćimovski D, Bezbradica D. Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module. in Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain. 2023;:84-85.
https://hdl.handle.net/21.15107/rcub_technorep_6871 .

Veljković, Milica, Simović, Milica, Banjanac, Katarina, Milivojević, Ana, Ćorović, Marija, Jaćimovski, Darko, Bezbradica, Dejan, "Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module" in Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain (2023):84-85,
https://hdl.handle.net/21.15107/rcub_technorep_6871 .

TY  - JOUR
AU  - Pravilović, Radoslava
AU  - Janković, Tamara
AU  - Veljković, Milica
AU  - Todić, Branislav
AU  - Simović, Milica
AU  - Bezbradica, Dejan
AU  - Nikačević, Nikola
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6385
AB  - A new micro-kinetic model of the enzyme-catalyzed synthesis of fructo-oligosaccharides (FOS) was developed. A commercial enzyme mixture Pectinex® Ultra SP-L derived from Aspergillus aculeatus was used. A variety of initial enzyme concentrations (1–5 vol%) and sucrose concentrations (400–600 g/L) were experimentally investigated and included in kinetic modeling. Several variations of kinetic mechanisms and corresponding models have been examined. A hybrid genetic algorithm was used to predict the kinetic parameters simultaneously for all experimental data. The best fitting model has been adopted, and with an average error of 13.34%, it describes the experimental data very well. The influence of initial concentrations on the conversion of sucrose and production of FOS is being carefully investigated. It was shown that the initial sucrose concentration significantly affects the highest level of FOS concentration, but the enzyme concentration controls the time at which maximum is reached as well as the rate of FOS decomposition.
PB  - John Wiley and Sons Inc.
T2  - AIChE Journal
T1  - Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products
IS  - 9
SP  - e18122
VL  - 69
DO  - 10.1002/aic.18122
ER  - 

@article{
author = "Pravilović, Radoslava and Janković, Tamara and Veljković, Milica and Todić, Branislav and Simović, Milica and Bezbradica, Dejan and Nikačević, Nikola",
year = "2023",
abstract = "A new micro-kinetic model of the enzyme-catalyzed synthesis of fructo-oligosaccharides (FOS) was developed. A commercial enzyme mixture Pectinex® Ultra SP-L derived from Aspergillus aculeatus was used. A variety of initial enzyme concentrations (1–5 vol%) and sucrose concentrations (400–600 g/L) were experimentally investigated and included in kinetic modeling. Several variations of kinetic mechanisms and corresponding models have been examined. A hybrid genetic algorithm was used to predict the kinetic parameters simultaneously for all experimental data. The best fitting model has been adopted, and with an average error of 13.34%, it describes the experimental data very well. The influence of initial concentrations on the conversion of sucrose and production of FOS is being carefully investigated. It was shown that the initial sucrose concentration significantly affects the highest level of FOS concentration, but the enzyme concentration controls the time at which maximum is reached as well as the rate of FOS decomposition.",
publisher = "John Wiley and Sons Inc.",
journal = "AIChE Journal",
title = "Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products",
number = "9",
pages = "e18122",
volume = "69",
doi = "10.1002/aic.18122"
}

Pravilović, R., Janković, T., Veljković, M., Todić, B., Simović, M., Bezbradica, D.,& Nikačević, N.. (2023). Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products. in AIChE Journal
John Wiley and Sons Inc.., 69(9), e18122.
https://doi.org/10.1002/aic.18122

Pravilović R, Janković T, Veljković M, Todić B, Simović M, Bezbradica D, Nikačević N. Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products. in AIChE Journal. 2023;69(9):e18122.
doi:10.1002/aic.18122 .

Pravilović, Radoslava, Janković, Tamara, Veljković, Milica, Todić, Branislav, Simović, Milica, Bezbradica, Dejan, Nikačević, Nikola, "Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products" in AIChE Journal, 69, no. 9 (2023):e18122,
https://doi.org/10.1002/aic.18122 . .

TY  - CONF
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Petrov Ivanković, Anja
AU  - Nikolić, Valentina
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6842
AB  - Recently, there is an increasing interest in utilizing biomass and waste potential for development of new products and materials. The example of this is sunflower meal, by-product of sunflower seed oil industry, that is currently used as a cheap component of animal feed owing to a poor protein content. The major drawback for their application is high content of complex carbohydrates (15.27% hemicellulose, 12.54% cellulose and 5.88% lignin). Therefore, extraction and enzymatic transformation of carbohydrates (primarily xylan) represent an attractive valorization approach. Since xylan extraction from the plant cell wall is highly restricted by lignin presence, different alkaline extraction methods have been proposed. This work is focused on xylan extraction using ethanol extraction and subsequent delignification approach. The ethanol extraction step yielded fraction rich in simple sugars, polyphenols (predominantly chlorogenic acid) and colorants that proved to have great prebiotic potential for application in skin products enabling proliferation of beneficial skin commensal and suppressing the pathogen one. Introduction of additional delignification step enabled high xylan yields (91.35%). This fraction was subjected to the enzymatic treatment using commercial xylanase (ROHALASE® SEP-VISCO) to obtain xylo-oligosaccharides (XOS), compounds that comprise of 2–10 xylose residues linked via β-1,4-glycosidic bonds with high prebiotic activity and excellent application properties (high temperature and pH stability, good sweetening power and low caloric value) for food industry. As result of the sunflower meal xylan treatment under optimum reaction conditions (60°C, pH6 and enzyme concentration of 0.5% per substrate), XOS with a polymerization degree of 2–6 were produced (with predominance of XOS2), without xylose generation. The prebiotic activity of the obtained XOS fraction was confirmed by fermentation with beneficial microbial cultures and pathogenic gut microbiota representative. Therefore, it was shown that significant portion of meal can be converted to added-value functional ingredients, while remaining product retains higher protein content.
PB  - Belgrade : University, Faculty of Geography
C3  - Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023
T1  - Valorization of sunflower meal toward the production of emerging prebiotics
SP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6842
ER  - 

@conference{
author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Petrov Ivanković, Anja and Nikolić, Valentina and Bezbradica, Dejan",
year = "2023",
abstract = "Recently, there is an increasing interest in utilizing biomass and waste potential for development of new products and materials. The example of this is sunflower meal, by-product of sunflower seed oil industry, that is currently used as a cheap component of animal feed owing to a poor protein content. The major drawback for their application is high content of complex carbohydrates (15.27% hemicellulose, 12.54% cellulose and 5.88% lignin). Therefore, extraction and enzymatic transformation of carbohydrates (primarily xylan) represent an attractive valorization approach. Since xylan extraction from the plant cell wall is highly restricted by lignin presence, different alkaline extraction methods have been proposed. This work is focused on xylan extraction using ethanol extraction and subsequent delignification approach. The ethanol extraction step yielded fraction rich in simple sugars, polyphenols (predominantly chlorogenic acid) and colorants that proved to have great prebiotic potential for application in skin products enabling proliferation of beneficial skin commensal and suppressing the pathogen one. Introduction of additional delignification step enabled high xylan yields (91.35%). This fraction was subjected to the enzymatic treatment using commercial xylanase (ROHALASE® SEP-VISCO) to obtain xylo-oligosaccharides (XOS), compounds that comprise of 2–10 xylose residues linked via β-1,4-glycosidic bonds with high prebiotic activity and excellent application properties (high temperature and pH stability, good sweetening power and low caloric value) for food industry. As result of the sunflower meal xylan treatment under optimum reaction conditions (60°C, pH6 and enzyme concentration of 0.5% per substrate), XOS with a polymerization degree of 2–6 were produced (with predominance of XOS2), without xylose generation. The prebiotic activity of the obtained XOS fraction was confirmed by fermentation with beneficial microbial cultures and pathogenic gut microbiota representative. Therefore, it was shown that significant portion of meal can be converted to added-value functional ingredients, while remaining product retains higher protein content.",
publisher = "Belgrade : University, Faculty of Geography",
journal = "Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023",
title = "Valorization of sunflower meal toward the production of emerging prebiotics",
pages = "36",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6842"
}

Veljković, M., Simović, M., Banjanac, K., Petrov Ivanković, A., Nikolić, V.,& Bezbradica, D.. (2023). Valorization of sunflower meal toward the production of emerging prebiotics. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023
Belgrade : University, Faculty of Geography., 36.
https://hdl.handle.net/21.15107/rcub_technorep_6842

Veljković M, Simović M, Banjanac K, Petrov Ivanković A, Nikolić V, Bezbradica D. Valorization of sunflower meal toward the production of emerging prebiotics. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023. 2023;:36.
https://hdl.handle.net/21.15107/rcub_technorep_6842 .

Veljković, Milica, Simović, Milica, Banjanac, Katarina, Petrov Ivanković, Anja, Nikolić, Valentina, Bezbradica, Dejan, "Valorization of sunflower meal toward the production of emerging prebiotics" in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023 (2023):36,
https://hdl.handle.net/21.15107/rcub_technorep_6842 .

TY  - PAT
AU  - Simović, Milica
AU  - Bezbradica, Dejan
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5990
AB  - Predmetni pronalazak se odnosi na "in situ" enzimski
postupak sinteze frukto-oligosaharida direktnom
transfruktozilacijom saharoze prisutne u prehrambenim
proizvodima korišćenjem enzima fruktozil-transferaze. Na taj
način sharoza prisutna u proizvodu se konvertuje u
prebiotike, frukto-oligosaharide, pri čemu se dobija proizvod
poboljšanih funkcionalnih svojstava, sa smanjenim sadržajem
saharoze, tj. manjim glikemijskim indeksom. Takođe,
predmetni postupak se odnosi i na proizvode sa prebiotskom
aktivnošću proizvedene prema metodama ovog pronalaska
PB  - Гласник интелектуалне својине
T2  - Glasnik intelektualne svojine
T1  - Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics
IS  - 3
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5990
ER  - 

@misc{
author = "Simović, Milica and Bezbradica, Dejan and Ćorović, Marija and Milivojević, Ana and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "Predmetni pronalazak se odnosi na "in situ" enzimski
postupak sinteze frukto-oligosaharida direktnom
transfruktozilacijom saharoze prisutne u prehrambenim
proizvodima korišćenjem enzima fruktozil-transferaze. Na taj
način sharoza prisutna u proizvodu se konvertuje u
prebiotike, frukto-oligosaharide, pri čemu se dobija proizvod
poboljšanih funkcionalnih svojstava, sa smanjenim sadržajem
saharoze, tj. manjim glikemijskim indeksom. Takođe,
predmetni postupak se odnosi i na proizvode sa prebiotskom
aktivnošću proizvedene prema metodama ovog pronalaska",
publisher = "Гласник интелектуалне својине",
journal = "Glasnik intelektualne svojine",
title = "Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics",
number = "3",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5990"
}

Simović, M., Bezbradica, D., Ćorović, M., Milivojević, A.,& Dimitrijević-Branković, S.. (2022). Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics. in Glasnik intelektualne svojine
Гласник интелектуалне својине.(3), 8.
https://hdl.handle.net/21.15107/rcub_technorep_5990

Simović M, Bezbradica D, Ćorović M, Milivojević A, Dimitrijević-Branković S. Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics. in Glasnik intelektualne svojine. 2022;(3):8.
https://hdl.handle.net/21.15107/rcub_technorep_5990 .

Simović, Milica, Bezbradica, Dejan, Ćorović, Marija, Milivojević, Ana, Dimitrijević-Branković, Suzana, "Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics" in Glasnik intelektualne svojine, no. 3 (2022):8,
https://hdl.handle.net/21.15107/rcub_technorep_5990 .

TY  - CONF
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Petrov Ivanković, Anja
AU  - Vukoičić, Ana
AU  - Banjanac, Katarina
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5993
AB  - It is well known that maple syrup, a product obtained by processing maple tree sap, is widely
used in many households due to its sweetness and beneficial ingredients. It is most often used
as a topping for pancakes, waffles, donuts, and other desserts, but also as a sweetener for
numerous beverages such as coffee, tea, lemonade, and many others. This natural syrup, in
which, in addition to numerous minerals, antioxidants, carbohydrates and other components,
sucrose predominates, presents an excellent substrate for the synthesis of bioactive
molecules, i.e. fructo-oligosaccharides (FOS). These indigestible oligosaccharides which
belong to a group of established prebiotics are very suitable from the standpoint of human
health because they enable the normal functioning of the gastrointestinal tract, have a
positive effect on the immune system as well as many other benefits for the human organism.
With the approach which include the conversion of sucrose into FOS, it is possible to obtain a
product with improved characteristics, i.e. higher functional and lower caloric values.
Accordingly, in this study, a detailed optimization of the enzymatic synthesis of FOS was
performed using maple syrup as a source of sucrose and commercial enzyme mixture
Pectinex® Ultra SP-L as a source of fructosyltransferase. Namely, by individual varying of
enzymatic synthesis key factors such as temperature (30-80 °C), enzyme concentration (1, 3
and 5%) and reaction time (0-25 h), optimal conditions were selected. It was determined that
by performing the synthesis reaction at a temperature of 60 °C with an enzyme concentration
of 3% for 12h, a remarkable sucrose hydrolysis degree of 82% and a FOS yield of approximately 55% were achieved. In this case, the estimated caloric value of the obtained
product is about 2.2 kcal/mL, which is around 1.6-fold lower compared to the initial value of
3.6 kcal/mL. The product obtained in this manner could represent a low-calorie sweetener
with high fiber content and could potentially be included in the diet of diabetics and obese
people, as well as all those who aim to maintain better overall health
PB  - Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia
C3  - 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts
T1  - In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product
EP  - 153
SP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5993
ER  - 

@conference{
author = "Veljković, Milica and Simović, Milica and Petrov Ivanković, Anja and Vukoičić, Ana and Banjanac, Katarina and Bezbradica, Dejan",
year = "2022",
abstract = "It is well known that maple syrup, a product obtained by processing maple tree sap, is widely
used in many households due to its sweetness and beneficial ingredients. It is most often used
as a topping for pancakes, waffles, donuts, and other desserts, but also as a sweetener for
numerous beverages such as coffee, tea, lemonade, and many others. This natural syrup, in
which, in addition to numerous minerals, antioxidants, carbohydrates and other components,
sucrose predominates, presents an excellent substrate for the synthesis of bioactive
molecules, i.e. fructo-oligosaccharides (FOS). These indigestible oligosaccharides which
belong to a group of established prebiotics are very suitable from the standpoint of human
health because they enable the normal functioning of the gastrointestinal tract, have a
positive effect on the immune system as well as many other benefits for the human organism.
With the approach which include the conversion of sucrose into FOS, it is possible to obtain a
product with improved characteristics, i.e. higher functional and lower caloric values.
Accordingly, in this study, a detailed optimization of the enzymatic synthesis of FOS was
performed using maple syrup as a source of sucrose and commercial enzyme mixture
Pectinex® Ultra SP-L as a source of fructosyltransferase. Namely, by individual varying of
enzymatic synthesis key factors such as temperature (30-80 °C), enzyme concentration (1, 3
and 5%) and reaction time (0-25 h), optimal conditions were selected. It was determined that
by performing the synthesis reaction at a temperature of 60 °C with an enzyme concentration
of 3% for 12h, a remarkable sucrose hydrolysis degree of 82% and a FOS yield of approximately 55% were achieved. In this case, the estimated caloric value of the obtained
product is about 2.2 kcal/mL, which is around 1.6-fold lower compared to the initial value of
3.6 kcal/mL. The product obtained in this manner could represent a low-calorie sweetener
with high fiber content and could potentially be included in the diet of diabetics and obese
people, as well as all those who aim to maintain better overall health",
publisher = "Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia",
journal = "10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts",
title = "In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product",
pages = "153-152",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5993"
}

Veljković, M., Simović, M., Petrov Ivanković, A., Vukoičić, A., Banjanac, K.,& Bezbradica, D.. (2022). In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product. in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts
Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia., 152-153.
https://hdl.handle.net/21.15107/rcub_technorep_5993

Veljković M, Simović M, Petrov Ivanković A, Vukoičić A, Banjanac K, Bezbradica D. In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product. in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts. 2022;:152-153.
https://hdl.handle.net/21.15107/rcub_technorep_5993 .

Veljković, Milica, Simović, Milica, Petrov Ivanković, Anja, Vukoičić, Ana, Banjanac, Katarina, Bezbradica, Dejan, "In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product" in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts (2022):152-153,
https://hdl.handle.net/21.15107/rcub_technorep_5993 .

TY  - CONF
AU  - Petrov Ivanković, Anja
AU  - Veljković, Milica
AU  - Vukoičić, Ana
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5994
AB  - According to experts, berries are a major source of phytochemicals, disease-fighting
components, which can help to boost the immune system and lower the risk of many agingrelated conditions. The simplest way to isolate these compounds is through solvent
extraction. However, this method is not entirely successful since, in addition to free
phytochemicals, there are also significant amounts of bound components that are trapped in
the cell wall. For these reasons, the addition of enzymes such as cellulases and pectinases
provides the hydrolysis of the cell wall, facilitate the release of molecules and increase the
extraction yield of phytochemical. Therefore, in this work, enzyme-assisted extraction of
lyophilized blackcurrant (LCR), a fruit that is increasingly used for pharmaceutical and
cosmetic purposes, was examined. Extraction was performed in acetate buffer pH 4.5, at 50 ̊
C using four enzymes: Pectinex® Ultra SP-L, Viscozyme® L, Cellic® CTec3, Rohapect® MC. In
the first step of the experiment, the extraction was optimized by different-time varying, from
10 to 120 min, and after choosing the optimal time, the influence of the solid-liquid ratio (1:4,
1:10, 1:40) on the extraction efficiency was examined. The progress of extraction was
monitored spectrophotometrically, through antioxidant properties, the total content of
polyphenols, flavonoids, and phenolic acids from all blackcurrant extracts and compared to
the control (extract without enzyme). Regarding the total content of polyphenols, it was
revealed that 60 min was the optimal extraction time. On the other hand, it was found that for a solid-liquid ratio of 1:4, all results were about 1.5 times higher than the control values.
In terms of enzymes, Viscozyme® and Pectinex® Ultra SP-L enabled the recovery of the highest
total phenolic content (~28 mg GAE/g LCR) and phenolic acids (~3 mg CA/g LCR), while, on the
other hand, Rohapect® MC enhanced the extraction of antioxidants (FRAP 380 µmol Fe2+/g
LCR), as well as total flavonoids (2.85 mg QE/g LCR). Obtained results open new possibilities
for blackcurrant extracts treated with different enzymes as value-added ingredients for the
cosmetic and food industry
PB  - Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia
C3  - 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts
T1  - Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum)
EP  - 146
SP  - 145
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5994
ER  - 

@conference{
author = "Petrov Ivanković, Anja and Veljković, Milica and Vukoičić, Ana and Milivojević, Ana and Ćorović, Marija and Pjanović, Rada and Bezbradica, Dejan",
year = "2022",
abstract = "According to experts, berries are a major source of phytochemicals, disease-fighting
components, which can help to boost the immune system and lower the risk of many agingrelated conditions. The simplest way to isolate these compounds is through solvent
extraction. However, this method is not entirely successful since, in addition to free
phytochemicals, there are also significant amounts of bound components that are trapped in
the cell wall. For these reasons, the addition of enzymes such as cellulases and pectinases
provides the hydrolysis of the cell wall, facilitate the release of molecules and increase the
extraction yield of phytochemical. Therefore, in this work, enzyme-assisted extraction of
lyophilized blackcurrant (LCR), a fruit that is increasingly used for pharmaceutical and
cosmetic purposes, was examined. Extraction was performed in acetate buffer pH 4.5, at 50 ̊
C using four enzymes: Pectinex® Ultra SP-L, Viscozyme® L, Cellic® CTec3, Rohapect® MC. In
the first step of the experiment, the extraction was optimized by different-time varying, from
10 to 120 min, and after choosing the optimal time, the influence of the solid-liquid ratio (1:4,
1:10, 1:40) on the extraction efficiency was examined. The progress of extraction was
monitored spectrophotometrically, through antioxidant properties, the total content of
polyphenols, flavonoids, and phenolic acids from all blackcurrant extracts and compared to
the control (extract without enzyme). Regarding the total content of polyphenols, it was
revealed that 60 min was the optimal extraction time. On the other hand, it was found that for a solid-liquid ratio of 1:4, all results were about 1.5 times higher than the control values.
In terms of enzymes, Viscozyme® and Pectinex® Ultra SP-L enabled the recovery of the highest
total phenolic content (~28 mg GAE/g LCR) and phenolic acids (~3 mg CA/g LCR), while, on the
other hand, Rohapect® MC enhanced the extraction of antioxidants (FRAP 380 µmol Fe2+/g
LCR), as well as total flavonoids (2.85 mg QE/g LCR). Obtained results open new possibilities
for blackcurrant extracts treated with different enzymes as value-added ingredients for the
cosmetic and food industry",
publisher = "Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia",
journal = "10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts",
title = "Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum)",
pages = "146-145",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5994"
}

Petrov Ivanković, A., Veljković, M., Vukoičić, A., Milivojević, A., Ćorović, M., Pjanović, R.,& Bezbradica, D.. (2022). Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum). in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts
Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia., 145-146.
https://hdl.handle.net/21.15107/rcub_technorep_5994

Petrov Ivanković A, Veljković M, Vukoičić A, Milivojević A, Ćorović M, Pjanović R, Bezbradica D. Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum). in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts. 2022;:145-146.
https://hdl.handle.net/21.15107/rcub_technorep_5994 .

Petrov Ivanković, Anja, Veljković, Milica, Vukoičić, Ana, Milivojević, Ana, Ćorović, Marija, Pjanović, Rada, Bezbradica, Dejan, "Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum)" in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts (2022):145-146,
https://hdl.handle.net/21.15107/rcub_technorep_5994 .

TY  - JOUR
AU  - Petrov, Anja
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5141
AB  - Recently, prebiotics are attracting plenty of attention in the field of skin care, since it is found that they are able to support the balance of beneficial and harmful microorganisms on the skin, and accordingly prevent several skin conditions associated with microbial imbalance. Topical application of prebiotics, although insufficiently investigated, holds great promise in improving skin health. The purpose of this research was to determine the prebiotic potential of galacto-oligosaccharides (GOS) for skin microbiota and suitability for incorporation in different topical formulations, and finally, provide insights into their diffusion properties. Methods: The prebiotic effect of GOS was evaluated through the influence on the growth of Staphylococcus epidermidis and Staphylococcus aureus, the most common resident and pathogenic bacterium of the skin microbiota, respectively. Also, with the future use of GOS in cosmetic products in mind, the diffusion of GOS molecules from two different topical formulations, hydrogel and oil-in-water (O/W) gel emulsion, was monitored employing Franz diffusion cell and two systems—with cellulose acetate membrane and transdermal diffusion test model, Strat-M® membrane. Course of fermentation and the amount of diffused GOS molecules were monitored using high-performance liquid chromatography (HPLC). Results: The in vitro results revealed that GOS at a concentration of 5% (w/v) has a pronounced stimulatory effect on S. epidermidis, while simultaneously showing an inhibitory effect on S. aureus, both in nutrient broth and cosmetic formulations. GOS trisaccharide and tetrasaccharide diffusion coefficients from O/W gel emulsion were calculated to be 5.61·10−6 cm2 s−1 and 1.41·10−8 cm2 s−1, respectively. The diffusion coefficient of GOS trisaccharides from hydrogel was 3.22·10−6 cm2 s−1, while it was not determined for tetrasaccharides due to low diffused concentration. Transdermal diffusion tests revealed that GOS incorporated in two formulations stays at the surface of the skin even after 24 h. Conclusion: When applied in adequate concentration, GOS has the potential to be used as a skin prebiotic. Novel GOS enriched formulations, Aristoflex® AVC-based hydrogel and Heliogel™-based O/W gel emulsion, provided efficient diffusion and delivery of prebiotic GOS molecules to the skin surface.
PB  - John Wiley and Sons Inc.
T2  - International Journal of Cosmetic Science
T1  - Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties
EP  - 319
IS  - 3
SP  - 309
VL  - 44
DO  - 10.1111/ics.12778
ER  - 

@article{
author = "Petrov, Anja and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Banjanac, Katarina and Pjanović, Rada and Bezbradica, Dejan",
year = "2022",
abstract = "Recently, prebiotics are attracting plenty of attention in the field of skin care, since it is found that they are able to support the balance of beneficial and harmful microorganisms on the skin, and accordingly prevent several skin conditions associated with microbial imbalance. Topical application of prebiotics, although insufficiently investigated, holds great promise in improving skin health. The purpose of this research was to determine the prebiotic potential of galacto-oligosaccharides (GOS) for skin microbiota and suitability for incorporation in different topical formulations, and finally, provide insights into their diffusion properties. Methods: The prebiotic effect of GOS was evaluated through the influence on the growth of Staphylococcus epidermidis and Staphylococcus aureus, the most common resident and pathogenic bacterium of the skin microbiota, respectively. Also, with the future use of GOS in cosmetic products in mind, the diffusion of GOS molecules from two different topical formulations, hydrogel and oil-in-water (O/W) gel emulsion, was monitored employing Franz diffusion cell and two systems—with cellulose acetate membrane and transdermal diffusion test model, Strat-M® membrane. Course of fermentation and the amount of diffused GOS molecules were monitored using high-performance liquid chromatography (HPLC). Results: The in vitro results revealed that GOS at a concentration of 5% (w/v) has a pronounced stimulatory effect on S. epidermidis, while simultaneously showing an inhibitory effect on S. aureus, both in nutrient broth and cosmetic formulations. GOS trisaccharide and tetrasaccharide diffusion coefficients from O/W gel emulsion were calculated to be 5.61·10−6 cm2 s−1 and 1.41·10−8 cm2 s−1, respectively. The diffusion coefficient of GOS trisaccharides from hydrogel was 3.22·10−6 cm2 s−1, while it was not determined for tetrasaccharides due to low diffused concentration. Transdermal diffusion tests revealed that GOS incorporated in two formulations stays at the surface of the skin even after 24 h. Conclusion: When applied in adequate concentration, GOS has the potential to be used as a skin prebiotic. Novel GOS enriched formulations, Aristoflex® AVC-based hydrogel and Heliogel™-based O/W gel emulsion, provided efficient diffusion and delivery of prebiotic GOS molecules to the skin surface.",
publisher = "John Wiley and Sons Inc.",
journal = "International Journal of Cosmetic Science",
title = "Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties",
pages = "319-309",
number = "3",
volume = "44",
doi = "10.1111/ics.12778"
}

Petrov, A., Ćorović, M., Milivojević, A., Simović, M., Banjanac, K., Pjanović, R.,& Bezbradica, D.. (2022). Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties. in International Journal of Cosmetic Science
John Wiley and Sons Inc.., 44(3), 309-319.
https://doi.org/10.1111/ics.12778

Petrov A, Ćorović M, Milivojević A, Simović M, Banjanac K, Pjanović R, Bezbradica D. Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties. in International Journal of Cosmetic Science. 2022;44(3):309-319.
doi:10.1111/ics.12778 .

Petrov, Anja, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Banjanac, Katarina, Pjanović, Rada, Bezbradica, Dejan, "Prebiotic effect of galacto-oligosaccharides on the skin microbiota and determination of their diffusion properties" in International Journal of Cosmetic Science, 44, no. 3 (2022):309-319,
https://doi.org/10.1111/ics.12778 . .