TY  - JOUR
AU  - Dimitrijević, Snežana
AU  - Milić, Marija
AU  - Tadić, Vanja
AU  - Maksimović, Svetolik
AU  - Filipović, Vladimir
AU  - Dimitrijević-Branković, Suzana
AU  - Miljković, Miona
AU  - Salamon, Ivan
PY  - 2024-02
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7036
AB  - Within this investigation, the effect of conventional and sophisticated extraction method was evaluated on the chemical composition of black cumin seeds (Nigella sativa L.) essential oils and their bioactive characteristics. Soxhlet extraction at 60 °C by hexane (SEH) and petroleum ether (SEP) was adopted as traditionally used technique, while the supercritical extraction with CO2 (SFE), under the pressure of 10 MPa and temperature of 40 °C, was performed as a modern method, expected to overcome the drawbacks of the conventional solvent extraction. The results showed that SFE enabled up to ten times higher extraction yields, compared to essential oils derived after Soxhlet extraction, however, the bioactive compounds content, such as polyphenols, flavonoids and carotenoids, were extracted in higher quantities in the oil from Soxhlet extraction performance. Thereby, the strongest antioxidant agent was found to be the essential oil obtained from SEP, followed by the oil from SFE and oil obtained from SEH, respectively. The GC-MS analysis revealed that the content of thymoquinone was the highest in the essential oil from SEP (69.58%), and the obtained values are among the highest reported within the available literature data. Also, this oil sample contained about eleven times higher thymoquinone quantities than the essential oil obtained by SFE, which was mostly constituted of a methyl linoleate, a fatty acid methyl ester of linoleic acid. In addition, the essential oil from SEP exerted a value added antimicrobial properties, where it was found to be particularly effective in suppression of the Gram-positive bacteria growth.
PB  - Elsevier B.V.
T2  - Sustainable Chemistry and Pharmacy
T1  - Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique
SP  - 101390
VL  - 37
DO  - 10.1016/j.scp.2023.101390
ER  - 

@article{
author = "Dimitrijević, Snežana and Milić, Marija and Tadić, Vanja and Maksimović, Svetolik and Filipović, Vladimir and Dimitrijević-Branković, Suzana and Miljković, Miona and Salamon, Ivan",
year = "2024-02",
abstract = "Within this investigation, the effect of conventional and sophisticated extraction method was evaluated on the chemical composition of black cumin seeds (Nigella sativa L.) essential oils and their bioactive characteristics. Soxhlet extraction at 60 °C by hexane (SEH) and petroleum ether (SEP) was adopted as traditionally used technique, while the supercritical extraction with CO2 (SFE), under the pressure of 10 MPa and temperature of 40 °C, was performed as a modern method, expected to overcome the drawbacks of the conventional solvent extraction. The results showed that SFE enabled up to ten times higher extraction yields, compared to essential oils derived after Soxhlet extraction, however, the bioactive compounds content, such as polyphenols, flavonoids and carotenoids, were extracted in higher quantities in the oil from Soxhlet extraction performance. Thereby, the strongest antioxidant agent was found to be the essential oil obtained from SEP, followed by the oil from SFE and oil obtained from SEH, respectively. The GC-MS analysis revealed that the content of thymoquinone was the highest in the essential oil from SEP (69.58%), and the obtained values are among the highest reported within the available literature data. Also, this oil sample contained about eleven times higher thymoquinone quantities than the essential oil obtained by SFE, which was mostly constituted of a methyl linoleate, a fatty acid methyl ester of linoleic acid. In addition, the essential oil from SEP exerted a value added antimicrobial properties, where it was found to be particularly effective in suppression of the Gram-positive bacteria growth.",
publisher = "Elsevier B.V.",
journal = "Sustainable Chemistry and Pharmacy",
title = "Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique",
pages = "101390",
volume = "37",
doi = "10.1016/j.scp.2023.101390"
}

Dimitrijević, S., Milić, M., Tadić, V., Maksimović, S., Filipović, V., Dimitrijević-Branković, S., Miljković, M.,& Salamon, I.. (2024-02). Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique. in Sustainable Chemistry and Pharmacy
Elsevier B.V.., 37, 101390.
https://doi.org/10.1016/j.scp.2023.101390

Dimitrijević S, Milić M, Tadić V, Maksimović S, Filipović V, Dimitrijević-Branković S, Miljković M, Salamon I. Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique. in Sustainable Chemistry and Pharmacy. 2024;37:101390.
doi:10.1016/j.scp.2023.101390 .

Dimitrijević, Snežana, Milić, Marija, Tadić, Vanja, Maksimović, Svetolik, Filipović, Vladimir, Dimitrijević-Branković, Suzana, Miljković, Miona, Salamon, Ivan, "Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique" in Sustainable Chemistry and Pharmacy, 37 (2024-02):101390,
https://doi.org/10.1016/j.scp.2023.101390 . .

TY  - JOUR
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Milić, Marija
AU  - Lađarević, Jelena
AU  - Onjia, Antonije
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7444
AB  - This study presents an innovative approach for the reuse and recycling of waste material, brewer’s spent grain (BSG) for
creating a novel green biocatalyst. The same BSG was utilized in several consecutive steps: initially, it served as a substrate
for the cultivation and production of laccase by a novel isolated fungal strain, Coriolopsis trogii 2SMKN, then, it was reused
as a carrier for laccase immobilization, aiding in the process of azo dye decolorization and fnally, reused as recycled BSG
for the second successful laccase immobilization for six guaiacol oxidation, contributing to a zero-waste strategy. The novel
fungal strain produced laccase with a maximum activity of 171.4 U/g after 6 days of solid-state fermentation using BSG
as a substrate. The obtained laccase exhibited excellent performance in the decolorization of azo dyes, both as a free and
immobilized, at high temperatures, without addition of harmful mediators, achieving maximum decolorization efciencies
of 99.0%, 71.2%, and 61.0% for Orange G (OG), Congo Red, and Eriochrome Black T (EBT), respectively. The immobilized
laccase on BSG was successfully reused across fve cycles of azo dye decolorization process. Notably, new green biocatalyst
outperformed commercial laccase from Aspergillus spp. in the decolorization of OG and EBT. GC-MS and LC-MS revealed
azo-dye degradation products and decomposition pathway. This analysis was complemented by antimicrobial and phytotoxicity tests, which confrmed the non-toxic nature of the degradation products, indicating the potential for safe environmental
disposal.
PB  - Springer
T2  - Environmental Science and Pollution Research
T1  - Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain
DO  - 10.1007/s11356-024-33367-x
ER  - 

@article{
author = "Ilić, Nevena and Davidović, Slađana and Milić, Marija and Lađarević, Jelena and Onjia, Antonije and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2024",
abstract = "This study presents an innovative approach for the reuse and recycling of waste material, brewer’s spent grain (BSG) for
creating a novel green biocatalyst. The same BSG was utilized in several consecutive steps: initially, it served as a substrate
for the cultivation and production of laccase by a novel isolated fungal strain, Coriolopsis trogii 2SMKN, then, it was reused
as a carrier for laccase immobilization, aiding in the process of azo dye decolorization and fnally, reused as recycled BSG
for the second successful laccase immobilization for six guaiacol oxidation, contributing to a zero-waste strategy. The novel
fungal strain produced laccase with a maximum activity of 171.4 U/g after 6 days of solid-state fermentation using BSG
as a substrate. The obtained laccase exhibited excellent performance in the decolorization of azo dyes, both as a free and
immobilized, at high temperatures, without addition of harmful mediators, achieving maximum decolorization efciencies
of 99.0%, 71.2%, and 61.0% for Orange G (OG), Congo Red, and Eriochrome Black T (EBT), respectively. The immobilized
laccase on BSG was successfully reused across fve cycles of azo dye decolorization process. Notably, new green biocatalyst
outperformed commercial laccase from Aspergillus spp. in the decolorization of OG and EBT. GC-MS and LC-MS revealed
azo-dye degradation products and decomposition pathway. This analysis was complemented by antimicrobial and phytotoxicity tests, which confrmed the non-toxic nature of the degradation products, indicating the potential for safe environmental
disposal.",
publisher = "Springer",
journal = "Environmental Science and Pollution Research",
title = "Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain",
doi = "10.1007/s11356-024-33367-x"
}

Ilić, N., Davidović, S., Milić, M., Lađarević, J., Onjia, A., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2024). Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain. in Environmental Science and Pollution Research
Springer..
https://doi.org/10.1007/s11356-024-33367-x

Ilić N, Davidović S, Milić M, Lađarević J, Onjia A, Dimitrijević-Branković S, Mihajlovski K. Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain. in Environmental Science and Pollution Research. 2024;.
doi:10.1007/s11356-024-33367-x .

Ilić, Nevena, Davidović, Slađana, Milić, Marija, Lađarević, Jelena, Onjia, Antonije, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain" in Environmental Science and Pollution Research (2024),
https://doi.org/10.1007/s11356-024-33367-x . .

TY  - JOUR
AU  - Dimitrijević, Snežana
AU  - Milić, Marija
AU  - Buntić, Aneta
AU  - Dimitrijević-Branković, Suzana
AU  - Filipović, Vladimir
AU  - Popović, Vera
AU  - Salamon, Ivan
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7344
AB  - Composting of mixed medicinal plant waste was performed with the addition of mixture of plant growth promoting bacteria (PGPB), including Streptomyces sp., Paenybacillus sp., Bacillus sp., and Hymenobacter sp., and portions of spent coffee grounds (SCG). To assess the maturity and quality parameters of the compost, chemical and biological parameters (C/N ratio, loss of organic matter, CO2 production, dehydrogenase activity (DHA), and phytotoxicity) were evaluated during the three months of the composting process. The results revealed that the control sample (without the addition of SCG and PGPB) had evidently lower values of DHA during the entire monitoring period in comparison to other samples, indicating lower microbial activity in the compost mixture. Also, according to the released CO2, the composting process was accelerated in all samples where the SCG and PGPB were added, enabling a reduction in time needed for mixed plant waste to decompose. The germination index (GI) of the tested seeds indicated that the produced compost was acceptable and safe, with regard to all of the evaluated samples. However, at the end of the process the samples contained SCG and PGPB, which caused an increase of more than 50% of the GI in comparison to the control sample, expressing a high phyto-stimulant effect and improving the biofertilization impact.
PB  - MDPI
T2  - Sustainability
T1  - Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost
IS  - 4
SP  - 1632
VL  - 16
DO  - 10.3390/su16041632
ER  - 

@article{
author = "Dimitrijević, Snežana and Milić, Marija and Buntić, Aneta and Dimitrijević-Branković, Suzana and Filipović, Vladimir and Popović, Vera and Salamon, Ivan",
year = "2024",
abstract = "Composting of mixed medicinal plant waste was performed with the addition of mixture of plant growth promoting bacteria (PGPB), including Streptomyces sp., Paenybacillus sp., Bacillus sp., and Hymenobacter sp., and portions of spent coffee grounds (SCG). To assess the maturity and quality parameters of the compost, chemical and biological parameters (C/N ratio, loss of organic matter, CO2 production, dehydrogenase activity (DHA), and phytotoxicity) were evaluated during the three months of the composting process. The results revealed that the control sample (without the addition of SCG and PGPB) had evidently lower values of DHA during the entire monitoring period in comparison to other samples, indicating lower microbial activity in the compost mixture. Also, according to the released CO2, the composting process was accelerated in all samples where the SCG and PGPB were added, enabling a reduction in time needed for mixed plant waste to decompose. The germination index (GI) of the tested seeds indicated that the produced compost was acceptable and safe, with regard to all of the evaluated samples. However, at the end of the process the samples contained SCG and PGPB, which caused an increase of more than 50% of the GI in comparison to the control sample, expressing a high phyto-stimulant effect and improving the biofertilization impact.",
publisher = "MDPI",
journal = "Sustainability",
title = "Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost",
number = "4",
pages = "1632",
volume = "16",
doi = "10.3390/su16041632"
}

Dimitrijević, S., Milić, M., Buntić, A., Dimitrijević-Branković, S., Filipović, V., Popović, V.,& Salamon, I.. (2024). Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. in Sustainability
MDPI., 16(4), 1632.
https://doi.org/10.3390/su16041632

Dimitrijević S, Milić M, Buntić A, Dimitrijević-Branković S, Filipović V, Popović V, Salamon I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. in Sustainability. 2024;16(4):1632.
doi:10.3390/su16041632 .

Dimitrijević, Snežana, Milić, Marija, Buntić, Aneta, Dimitrijević-Branković, Suzana, Filipović, Vladimir, Popović, Vera, Salamon, Ivan, "Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost" in Sustainability, 16, no. 4 (2024):1632,
https://doi.org/10.3390/su16041632 . .

TY  - DATA
AU  - Dimitrijević, Snežana
AU  - Milić, Marija
AU  - Buntić, Aneta
AU  - Dimitrijević-Branković, Suzana
AU  - Filipović, Vladimir
AU  - Popović, Vera
AU  - Salamon, Ivan
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7345
AB  - Composting of mixed medicinal plant waste was performed with the addition of mixture of plant growth promoting bacteria (PGPB), including Streptomyces sp., Paenybacillus sp., Bacillus sp., and Hymenobacter sp., and portions of spent coffee grounds (SCG). To assess the maturity and quality parameters of the compost, chemical and biological parameters (C/N ratio, loss of organic matter, CO2 production, dehydrogenase activity (DHA), and phytotoxicity) were evaluated during the three months of the composting process. The results revealed that the control sample (without the addition of SCG and PGPB) had evidently lower values of DHA during the entire monitoring period in comparison to other samples, indicating lower microbial activity in the compost mixture. Also, according to the released CO2, the composting process was accelerated in all samples where the SCG and PGPB were added, enabling a reduction in time needed for mixed plant waste to decompose. The germination index (GI) of the tested seeds indicated that the produced compost was acceptable and safe, with regard to all of the evaluated samples. However, at the end of the process the samples contained SCG and PGPB, which caused an increase of more than 50% of the GI in comparison to the control sample, expressing a high phyto-stimulant effect and improving the biofertilization impact.
PB  - MDPI
T2  - Sustainability
T1  - Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632
IS  - 4
SP  - 1632
VL  - 16
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7345
ER  - 

@misc{
author = "Dimitrijević, Snežana and Milić, Marija and Buntić, Aneta and Dimitrijević-Branković, Suzana and Filipović, Vladimir and Popović, Vera and Salamon, Ivan",
year = "2024",
abstract = "Composting of mixed medicinal plant waste was performed with the addition of mixture of plant growth promoting bacteria (PGPB), including Streptomyces sp., Paenybacillus sp., Bacillus sp., and Hymenobacter sp., and portions of spent coffee grounds (SCG). To assess the maturity and quality parameters of the compost, chemical and biological parameters (C/N ratio, loss of organic matter, CO2 production, dehydrogenase activity (DHA), and phytotoxicity) were evaluated during the three months of the composting process. The results revealed that the control sample (without the addition of SCG and PGPB) had evidently lower values of DHA during the entire monitoring period in comparison to other samples, indicating lower microbial activity in the compost mixture. Also, according to the released CO2, the composting process was accelerated in all samples where the SCG and PGPB were added, enabling a reduction in time needed for mixed plant waste to decompose. The germination index (GI) of the tested seeds indicated that the produced compost was acceptable and safe, with regard to all of the evaluated samples. However, at the end of the process the samples contained SCG and PGPB, which caused an increase of more than 50% of the GI in comparison to the control sample, expressing a high phyto-stimulant effect and improving the biofertilization impact.",
publisher = "MDPI",
journal = "Sustainability",
title = "Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632",
number = "4",
pages = "1632",
volume = "16",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7345"
}

Dimitrijević, S., Milić, M., Buntić, A., Dimitrijević-Branković, S., Filipović, V., Popović, V.,& Salamon, I.. (2024). Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632. in Sustainability
MDPI., 16(4), 1632.
https://hdl.handle.net/21.15107/rcub_technorep_7345

Dimitrijević S, Milić M, Buntić A, Dimitrijević-Branković S, Filipović V, Popović V, Salamon I. Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632. in Sustainability. 2024;16(4):1632.
https://hdl.handle.net/21.15107/rcub_technorep_7345 .

Dimitrijević, Snežana, Milić, Marija, Buntić, Aneta, Dimitrijević-Branković, Suzana, Filipović, Vladimir, Popović, Vera, Salamon, Ivan, "Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632" in Sustainability, 16, no. 4 (2024):1632,
https://hdl.handle.net/21.15107/rcub_technorep_7345 .

TY  - JOUR
AU  - Tošić, Miloš
AU  - Rajić, Vladimir
AU  - Pjević, Dejan
AU  - Stojadinović, Stevan
AU  - Krstulović, Nikša
AU  - Dimitrijević-Branković, Suzana
AU  - Momčilović, Miloš
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7412
AB  - This study proposes a simple and controlled method for producing TiO2 with phase junction, oxygen vacancies, and Ti3+ by combining picosecond pulsed laser irradiation and electrochemical anodization. Ti mesh was pretreated by irradiating with a picosecond pulsed laser technique using an Nd:YAG laser (1064 nm) at two fluencies, 15 J/cm2 and 30 J/cm2 . The samples were then subjected to electrochemical anodization to form TiO2 nanotube arrays on the previously laser-treated surface. This study will investigate the possibility of forming TiO2 nanotube arrays on a pre-laser-treated Ti substrate and determine their physicochemical and photocatalytic properties. The samples were characterized by FESEM, XRD, Raman, XPS, and UV-Vis DRS. UV-Vis spectroscopy was used to observe the progress of photocatalytic degradation for all samples, and degradation products were determined using GC-MS. With the synergistic effects of phase junction, oxygen vacancies, and Ti3+, the laser-treated TiO2 with 30 J/cm2 showed a higher photocatalytic degradation rate (85.1%) of the pesticide carbofuran compared to non-laser-treated TiO2 (54.8%), remaining stable during successive degradation cycles, which has promising practical applications.
T2  - Photonics
T1  - Synergy of Nd:YAG Picosecond Pulsed Laser Irradiation and Electrochemical Anodization in the Formation of TiO2 Nanostructures for the Photocatalytic Degradation of Pesticide Carbofuran
IS  - 3
SP  - 284
VL  - 11
DO  - 10.3390/photonics11030284
ER  - 

@article{
author = "Tošić, Miloš and Rajić, Vladimir and Pjević, Dejan and Stojadinović, Stevan and Krstulović, Nikša and Dimitrijević-Branković, Suzana and Momčilović, Miloš",
year = "2024",
abstract = "This study proposes a simple and controlled method for producing TiO2 with phase junction, oxygen vacancies, and Ti3+ by combining picosecond pulsed laser irradiation and electrochemical anodization. Ti mesh was pretreated by irradiating with a picosecond pulsed laser technique using an Nd:YAG laser (1064 nm) at two fluencies, 15 J/cm2 and 30 J/cm2 . The samples were then subjected to electrochemical anodization to form TiO2 nanotube arrays on the previously laser-treated surface. This study will investigate the possibility of forming TiO2 nanotube arrays on a pre-laser-treated Ti substrate and determine their physicochemical and photocatalytic properties. The samples were characterized by FESEM, XRD, Raman, XPS, and UV-Vis DRS. UV-Vis spectroscopy was used to observe the progress of photocatalytic degradation for all samples, and degradation products were determined using GC-MS. With the synergistic effects of phase junction, oxygen vacancies, and Ti3+, the laser-treated TiO2 with 30 J/cm2 showed a higher photocatalytic degradation rate (85.1%) of the pesticide carbofuran compared to non-laser-treated TiO2 (54.8%), remaining stable during successive degradation cycles, which has promising practical applications.",
journal = "Photonics",
title = "Synergy of Nd:YAG Picosecond Pulsed Laser Irradiation and Electrochemical Anodization in the Formation of TiO2 Nanostructures for the Photocatalytic Degradation of Pesticide Carbofuran",
number = "3",
pages = "284",
volume = "11",
doi = "10.3390/photonics11030284"
}

Tošić, M., Rajić, V., Pjević, D., Stojadinović, S., Krstulović, N., Dimitrijević-Branković, S.,& Momčilović, M.. (2024). Synergy of Nd:YAG Picosecond Pulsed Laser Irradiation and Electrochemical Anodization in the Formation of TiO2 Nanostructures for the Photocatalytic Degradation of Pesticide Carbofuran. in Photonics, 11(3), 284.
https://doi.org/10.3390/photonics11030284

Tošić M, Rajić V, Pjević D, Stojadinović S, Krstulović N, Dimitrijević-Branković S, Momčilović M. Synergy of Nd:YAG Picosecond Pulsed Laser Irradiation and Electrochemical Anodization in the Formation of TiO2 Nanostructures for the Photocatalytic Degradation of Pesticide Carbofuran. in Photonics. 2024;11(3):284.
doi:10.3390/photonics11030284 .

Tošić, Miloš, Rajić, Vladimir, Pjević, Dejan, Stojadinović, Stevan, Krstulović, Nikša, Dimitrijević-Branković, Suzana, Momčilović, Miloš, "Synergy of Nd:YAG Picosecond Pulsed Laser Irradiation and Electrochemical Anodization in the Formation of TiO2 Nanostructures for the Photocatalytic Degradation of Pesticide Carbofuran" in Photonics, 11, no. 3 (2024):284,
https://doi.org/10.3390/photonics11030284 . .

TY  - JOUR
AU  - Milić, Marija
AU  - Buntić, Aneta V.
AU  - Mihajlovski, Katarina
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4981
AB  - This study demonstrated the innovative biotransformation aspect of spent coffee grounds (SCG) by application of the specific enzymatic cocktail with addition of lactic acid bacteria strain Lactobacillus rhamnosus (ATCC (R) 7469 (TM)). This bioprocess induced the improvement of the most of the quality parameters in SCG, causing a considerable increase in the bioactive contents (total polyphenols, chlorogenic acid, reducing sugars, free amino acids) and antioxidant activity, in comparison to the non-fermented SCG. Under the optimal conditions, at 37 degrees C, for 24 h, with 5% v/w of the inoculum employed, the polyphenols were increased for 67% (227.3 +/- 3.3 mg GAE/g dm extract), reducing sugars 57% (277.9 +/- 7.6 mg glucose/g dm extract), and alpha-amino nitrogen 80% (161.2 +/- 9.8 mg/100 g dm extract), and the chlorogenic acid content was doubled, while the caffeine was reduced for 38%. Furthermore, the resulting product evinced a strong antioxidant activity detected via inhibition of DPPH radicals and reducing capacity assay (FRAP). The proposed technology is viable for a large-scale commercialization for utilization of easily accessible industrial residues, deriving a product with a nutritive formulation very desirable to be incorporated as an additive in food and feed industry.
T2  - Biomass Conversion and Biorefinery
T1  - The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization
EP  - 6759
IS  - 8
SP  - 6747
VL  - 13
DO  - 10.1007/s13399-021-01605-8
ER  - 

@article{
author = "Milić, Marija and Buntić, Aneta V. and Mihajlovski, Katarina and Ilić, Nevena and Davidović, Slađana and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "This study demonstrated the innovative biotransformation aspect of spent coffee grounds (SCG) by application of the specific enzymatic cocktail with addition of lactic acid bacteria strain Lactobacillus rhamnosus (ATCC (R) 7469 (TM)). This bioprocess induced the improvement of the most of the quality parameters in SCG, causing a considerable increase in the bioactive contents (total polyphenols, chlorogenic acid, reducing sugars, free amino acids) and antioxidant activity, in comparison to the non-fermented SCG. Under the optimal conditions, at 37 degrees C, for 24 h, with 5% v/w of the inoculum employed, the polyphenols were increased for 67% (227.3 +/- 3.3 mg GAE/g dm extract), reducing sugars 57% (277.9 +/- 7.6 mg glucose/g dm extract), and alpha-amino nitrogen 80% (161.2 +/- 9.8 mg/100 g dm extract), and the chlorogenic acid content was doubled, while the caffeine was reduced for 38%. Furthermore, the resulting product evinced a strong antioxidant activity detected via inhibition of DPPH radicals and reducing capacity assay (FRAP). The proposed technology is viable for a large-scale commercialization for utilization of easily accessible industrial residues, deriving a product with a nutritive formulation very desirable to be incorporated as an additive in food and feed industry.",
journal = "Biomass Conversion and Biorefinery",
title = "The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization",
pages = "6759-6747",
number = "8",
volume = "13",
doi = "10.1007/s13399-021-01605-8"
}

Milić, M., Buntić, A. V., Mihajlovski, K., Ilić, N., Davidović, S.,& Dimitrijević-Branković, S.. (2023). The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization. in Biomass Conversion and Biorefinery, 13(8), 6747-6759.
https://doi.org/10.1007/s13399-021-01605-8

Milić M, Buntić AV, Mihajlovski K, Ilić N, Davidović S, Dimitrijević-Branković S. The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization. in Biomass Conversion and Biorefinery. 2023;13(8):6747-6759.
doi:10.1007/s13399-021-01605-8 .

Milić, Marija, Buntić, Aneta V., Mihajlovski, Katarina, Ilić, Nevena, Davidović, Slađana, Dimitrijević-Branković, Suzana, "The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization" in Biomass Conversion and Biorefinery, 13, no. 8 (2023):6747-6759,
https://doi.org/10.1007/s13399-021-01605-8 . .

TY  - JOUR
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Beluhan, Sunčica
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6374
AB  - Cellulases are enzymes that are attracting worldwide attention because of their ability to degrade cellulose in the lignocellulosic biomass and transform it into highly demanded bioethanol. The enzymatic hydrolysis of cellulose by cellulases into fermentable sugars is a crucial step in biofuel production, given the complex structure of lignocellulose. Due to cellulases’ unique ability to hydrolyze the very recaltricant nature of lignocellulosic biomass, the cellulase market demand is rapidly growing. Although cellulases have been used in industrial applications for decades, constant effort is being made in the field of enzyme innovation to develop cellulase mixtures/cocktails with improved performance. Given that the main producers of cellulases are of microbial origin, there is a constant need to isolate new microorganisms as potential producers of enzymes important for biofuel production. This review provides insight into current research on improving microbial cellulase production as well as the outlook for the cellulase market with commercial cellulase preparation involved in industrial bioethanol production.
PB  - MDPI
T2  - Energies
T1  - Cellulases: From Lignocellulosic Biomass to Improved Production
IS  - 8
SP  - 3598
VL  - 16
DO  - 10.3390/en16083598
ER  - 

@article{
author = "Ilić, Nevena and Milić, Marija and Beluhan, Sunčica and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "Cellulases are enzymes that are attracting worldwide attention because of their ability to degrade cellulose in the lignocellulosic biomass and transform it into highly demanded bioethanol. The enzymatic hydrolysis of cellulose by cellulases into fermentable sugars is a crucial step in biofuel production, given the complex structure of lignocellulose. Due to cellulases’ unique ability to hydrolyze the very recaltricant nature of lignocellulosic biomass, the cellulase market demand is rapidly growing. Although cellulases have been used in industrial applications for decades, constant effort is being made in the field of enzyme innovation to develop cellulase mixtures/cocktails with improved performance. Given that the main producers of cellulases are of microbial origin, there is a constant need to isolate new microorganisms as potential producers of enzymes important for biofuel production. This review provides insight into current research on improving microbial cellulase production as well as the outlook for the cellulase market with commercial cellulase preparation involved in industrial bioethanol production.",
publisher = "MDPI",
journal = "Energies",
title = "Cellulases: From Lignocellulosic Biomass to Improved Production",
number = "8",
pages = "3598",
volume = "16",
doi = "10.3390/en16083598"
}

Ilić, N., Milić, M., Beluhan, S.,& Dimitrijević-Branković, S.. (2023). Cellulases: From Lignocellulosic Biomass to Improved Production. in Energies
MDPI., 16(8), 3598.
https://doi.org/10.3390/en16083598

Ilić N, Milić M, Beluhan S, Dimitrijević-Branković S. Cellulases: From Lignocellulosic Biomass to Improved Production. in Energies. 2023;16(8):3598.
doi:10.3390/en16083598 .

Ilić, Nevena, Milić, Marija, Beluhan, Sunčica, Dimitrijević-Branković, Suzana, "Cellulases: From Lignocellulosic Biomass to Improved Production" in Energies, 16, no. 8 (2023):3598,
https://doi.org/10.3390/en16083598 . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6605
AB  - Industrial dye effluents that contain triphenylmethane dyes are environment-threatening problem. The triphenylmethane dyes are one of the largest dye’s group that is discharged in large quantities to water bodies. Removing of them with fungal enzymes is big scientific challenge. The fungal laccases are promising tool for removing of these dyes from water bodies. In this study, the potential of crude fungal laccase from genus Ganoderma in decolorization of triphenylmethane dye, crystal violet was investigated. The crude fungal enzyme was produced using agroindustrial lignocellulosic waste, cereal mix. The effect of different substrate masses (15g, 25g and 50 g) and Cu2+ ions concentrations (0.25 mM, 0.75 mM, 1.0 mM, 2.5 mM и 5.0 mM) on lccase production were investigated. The obtained crude fungal laccase with the highest activity was used for decolorization of crystal violet at different concentrations from 20 mg/L to 50 mg/L. The crude fungal laccase from genus Ganoderma had the highest activity of 47.43 U/g, when the substrate mass of 50 g was used for enyzme production. The lower masses of substrate (15g and 25g) induced lower laccase activities of 45.07 U/g and 46.69 U/g, respectively. The initial addition of Cu2+ ions in concentration of 0.25 mM to agroindustrial waste led to decrease of laccase activity compared to the laccase activity of laccase obtained from agroindustrial waste that didn’t contain Cu2+ ions. On the other hand, the increase of Cu2+ concentration to 1.0 mM in the substrate led to increase the laccase activity. The highest laccase activity of 47.53 U/g was obtained when  the 1.0 mM Cu2+ was added to substrate, while the decrease in laccase activity was observed when 5.0 mM Cu2+ was added to the agroindustrial waste. Decolorization of crystal violet at different concentrations (20 mg/L, 30 mg/L, 40 mg/L, 50 mg/L) was carred out at pH 5 and temperature of 50 ºC for 120 min. The highest decolorization efficiency of 14.42 % was obtained in the case of the lowest dye concentration (20 mg/L), while the lowest decolorization efficiency of 3.76 % was obtained when the highest dye concentration of 50 mg/L was decolorized with crude fungal laccase for 120 min. The obtained results show that fungal crude laccases can be used for decolorization of triphenylmethane dyes, but the detailed optimization is very important for obtaining relatively high decolorization efficiencies for short time.
PB  - Zvornik : University of East Sarajevo Faculty of Technology
C3  - Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23
T1  - APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET
EP  - 186
SP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6605
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2023",
abstract = "Industrial dye effluents that contain triphenylmethane dyes are environment-threatening problem. The triphenylmethane dyes are one of the largest dye’s group that is discharged in large quantities to water bodies. Removing of them with fungal enzymes is big scientific challenge. The fungal laccases are promising tool for removing of these dyes from water bodies. In this study, the potential of crude fungal laccase from genus Ganoderma in decolorization of triphenylmethane dye, crystal violet was investigated. The crude fungal enzyme was produced using agroindustrial lignocellulosic waste, cereal mix. The effect of different substrate masses (15g, 25g and 50 g) and Cu2+ ions concentrations (0.25 mM, 0.75 mM, 1.0 mM, 2.5 mM и 5.0 mM) on lccase production were investigated. The obtained crude fungal laccase with the highest activity was used for decolorization of crystal violet at different concentrations from 20 mg/L to 50 mg/L. The crude fungal laccase from genus Ganoderma had the highest activity of 47.43 U/g, when the substrate mass of 50 g was used for enyzme production. The lower masses of substrate (15g and 25g) induced lower laccase activities of 45.07 U/g and 46.69 U/g, respectively. The initial addition of Cu2+ ions in concentration of 0.25 mM to agroindustrial waste led to decrease of laccase activity compared to the laccase activity of laccase obtained from agroindustrial waste that didn’t contain Cu2+ ions. On the other hand, the increase of Cu2+ concentration to 1.0 mM in the substrate led to increase the laccase activity. The highest laccase activity of 47.53 U/g was obtained when  the 1.0 mM Cu2+ was added to substrate, while the decrease in laccase activity was observed when 5.0 mM Cu2+ was added to the agroindustrial waste. Decolorization of crystal violet at different concentrations (20 mg/L, 30 mg/L, 40 mg/L, 50 mg/L) was carred out at pH 5 and temperature of 50 ºC for 120 min. The highest decolorization efficiency of 14.42 % was obtained in the case of the lowest dye concentration (20 mg/L), while the lowest decolorization efficiency of 3.76 % was obtained when the highest dye concentration of 50 mg/L was decolorized with crude fungal laccase for 120 min. The obtained results show that fungal crude laccases can be used for decolorization of triphenylmethane dyes, but the detailed optimization is very important for obtaining relatively high decolorization efficiencies for short time.",
publisher = "Zvornik : University of East Sarajevo Faculty of Technology",
journal = "Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23",
title = "APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET",
pages = "186-179",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6605"
}

Ilić, N., Milić, M., Davidović, S., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2023). APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23
Zvornik : University of East Sarajevo Faculty of Technology., 179-186.
https://hdl.handle.net/21.15107/rcub_technorep_6605

Ilić N, Milić M, Davidović S, Dimitrijević-Branković S, Mihajlovski K. APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23. 2023;:179-186.
https://hdl.handle.net/21.15107/rcub_technorep_6605 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET" in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23 (2023):179-186,
https://hdl.handle.net/21.15107/rcub_technorep_6605 .

TY  - CONF
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Radovanović, Neda
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6943
AB  - In recent decades, enzyme immobilization on different supports occupies an important place in the modern biotechnology, given that it allows the design of green and sustainable production processes. Nanoparticles are very efficient supports for enzyme immobilization. The enzyme immobilized on nanoparticles is more stable than its soluble form and can be used in several operative cycles. In this study, the potential of modified titanium dioxide nanoparticles (Degussa P25, TiO2) for crude fungal laccase (47.43 U/g) immobilization was investigated. The TiO2 nanoparticles were modified with (3-glycidyloxypropyl) trimetoxylane under different conditions in order to obtain support with an optimal concentration of epoxy groups for immobilization. The obtained TiO2 nanoparticles had different concentrations of epoxy groups on their surface, exactly 950 μmol/g (TiO2M1) and 500 μmol/g (TiO2M2), respectively. The immobilization was carried out at pH 5 and at room temperature for 4 h. The residual activity of immobilized laccase on TiO2M2 was 33.40 %, while the residual activity of immobilized laccase on TiO2M1 was 17.39 %. The immobilization efficiency was 26.75 % and 20.18 % for laccase immobilized on TiO2M2 and immobilized laccase on TiO2M1, respectively. The immobilization of crude fungal laccase on TiO2M2 was further optimized by testing the influence of different contact time between laccase and TiO2M2 (2 h ,3 h, 3,5 h and 4 h) as well as the effect of various pH values (pH 4, pH 5 and pH 6) on immobilization. The highest residual activity of 35.04 % and immobilization efficiency of 28.95 % were obtained for optimal contact time of 3.5 h between crude fungal laccase and TiO2M2. The lowest residual activity (30.38 %) and immobilization efficiency (20.18 %) were obtained when immobilization was carried out at room temperature and at pH 5 for 2 h. The pH values had significant effect on immobilization. The optimal pH value was pH 5 with highest residual activity of 35.04 %, while the lowest residual activity of 14. 83 % was at pH 6. The residual activity of 21.21 % and immobilization efficency of 19.52 % were obtained when crude fungal laccase immobilized at pH 4. The immobilized laccase on TiO2M2 was successfully used in 5 cycles of guaiacol oxidation. In the 2nd cycle, the immobilized laccase had residual activity of about 60%, while after 3rd cycle, immobilized laccase had residual activity of 40 %. The lowest residual activity of 14.83 %, immobilized laccase had after 5th cycle.
PB  - Zvornik : University of East Sarajevo Faculty of Technology
C3  - Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023
T1  - IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES
EP  - 196
SP  - 187
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6943
ER  - 

@conference{
author = "Ilić, Nevena and Davidović, Slađana and Miljković, Miona and Radovanović, Neda and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2023",
abstract = "In recent decades, enzyme immobilization on different supports occupies an important place in the modern biotechnology, given that it allows the design of green and sustainable production processes. Nanoparticles are very efficient supports for enzyme immobilization. The enzyme immobilized on nanoparticles is more stable than its soluble form and can be used in several operative cycles. In this study, the potential of modified titanium dioxide nanoparticles (Degussa P25, TiO2) for crude fungal laccase (47.43 U/g) immobilization was investigated. The TiO2 nanoparticles were modified with (3-glycidyloxypropyl) trimetoxylane under different conditions in order to obtain support with an optimal concentration of epoxy groups for immobilization. The obtained TiO2 nanoparticles had different concentrations of epoxy groups on their surface, exactly 950 μmol/g (TiO2M1) and 500 μmol/g (TiO2M2), respectively. The immobilization was carried out at pH 5 and at room temperature for 4 h. The residual activity of immobilized laccase on TiO2M2 was 33.40 %, while the residual activity of immobilized laccase on TiO2M1 was 17.39 %. The immobilization efficiency was 26.75 % and 20.18 % for laccase immobilized on TiO2M2 and immobilized laccase on TiO2M1, respectively. The immobilization of crude fungal laccase on TiO2M2 was further optimized by testing the influence of different contact time between laccase and TiO2M2 (2 h ,3 h, 3,5 h and 4 h) as well as the effect of various pH values (pH 4, pH 5 and pH 6) on immobilization. The highest residual activity of 35.04 % and immobilization efficiency of 28.95 % were obtained for optimal contact time of 3.5 h between crude fungal laccase and TiO2M2. The lowest residual activity (30.38 %) and immobilization efficiency (20.18 %) were obtained when immobilization was carried out at room temperature and at pH 5 for 2 h. The pH values had significant effect on immobilization. The optimal pH value was pH 5 with highest residual activity of 35.04 %, while the lowest residual activity of 14. 83 % was at pH 6. The residual activity of 21.21 % and immobilization efficency of 19.52 % were obtained when crude fungal laccase immobilized at pH 4. The immobilized laccase on TiO2M2 was successfully used in 5 cycles of guaiacol oxidation. In the 2nd cycle, the immobilized laccase had residual activity of about 60%, while after 3rd cycle, immobilized laccase had residual activity of 40 %. The lowest residual activity of 14.83 %, immobilized laccase had after 5th cycle.",
publisher = "Zvornik : University of East Sarajevo Faculty of Technology",
journal = "Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023",
title = "IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES",
pages = "196-187",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6943"
}

Ilić, N., Davidović, S., Miljković, M., Radovanović, N., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2023). IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023
Zvornik : University of East Sarajevo Faculty of Technology., 187-196.
https://hdl.handle.net/21.15107/rcub_technorep_6943

Ilić N, Davidović S, Miljković M, Radovanović N, Dimitrijević-Branković S, Mihajlovski K. IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023. 2023;:187-196.
https://hdl.handle.net/21.15107/rcub_technorep_6943 .

Ilić, Nevena, Davidović, Slađana, Miljković, Miona, Radovanović, Neda, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES" in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023 (2023):187-196,
https://hdl.handle.net/21.15107/rcub_technorep_6943 .

TY  - JOUR
AU  - Milivojević, Marija
AU  - Chen, Ke
AU  - Radovanović, Željko
AU  - Petrović, Rada
AU  - Dimitrijević-Branković, Suzana
AU  - Kojić, Vesna
AU  - Marković, Danica
AU  - Janaćković, Đorđe
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6803
AB  - The restoration of large bone defects caused by trauma, tumor resection, or infection is a major clinical problem in orthopedics and dentistry because postoperative infections, corrosion, and limited osteointegration of metal implants can lead to loosening of the implant. The aim of this study was to improve the surface properties of a 3D-printed (electron beam melting) Ti6Al4V-based macroporous scaffold by multilayer coating with bioactive silicate glasses (BAGs) and hydroxyapatite doped with a silver (AgHAP) or AgHAP additionally sonochemically modified with ZnO (ZnO-AgHAP). The coated scaffolds AgHAP_BAGs_Ti and ZnO-AgHAP_BAGs_Ti enhanced cytocompatibility in L929 and MRC5 cell lines and expressed bioactivity in simulated body fluid. A lower release of vanadium ions in coated samples compared to bare Ti scaffold indicates decreased dissolution of Ti alloy in coated samples. The coated samples reduced growth ofEscherichia coliandStaphylococcus aureusfor 4-6 orders of magnitude. Therefore, the 3D-printed Ti-based scaffolds coated with BAGs and (ZnO-)AgHAP have great potential for application as a multifunctional implant with antibacterial properties for the restoration of defects in load-bearing bones.
PB  - IOP Publishing
T2  - Biomedical Materials
T1  - Enhanced antimicrobial properties and bioactivity of 3D-printed titanium scaffolds by multilayer bioceramic coating for large bone defects
IS  - 6
SP  - 065020
VL  - 18
DO  - 10.1088/1748-605X/ad02d2
ER  - 

@article{
author = "Milivojević, Marija and Chen, Ke and Radovanović, Željko and Petrović, Rada and Dimitrijević-Branković, Suzana and Kojić, Vesna and Marković, Danica and Janaćković, Đorđe",
year = "2023",
abstract = "The restoration of large bone defects caused by trauma, tumor resection, or infection is a major clinical problem in orthopedics and dentistry because postoperative infections, corrosion, and limited osteointegration of metal implants can lead to loosening of the implant. The aim of this study was to improve the surface properties of a 3D-printed (electron beam melting) Ti6Al4V-based macroporous scaffold by multilayer coating with bioactive silicate glasses (BAGs) and hydroxyapatite doped with a silver (AgHAP) or AgHAP additionally sonochemically modified with ZnO (ZnO-AgHAP). The coated scaffolds AgHAP_BAGs_Ti and ZnO-AgHAP_BAGs_Ti enhanced cytocompatibility in L929 and MRC5 cell lines and expressed bioactivity in simulated body fluid. A lower release of vanadium ions in coated samples compared to bare Ti scaffold indicates decreased dissolution of Ti alloy in coated samples. The coated samples reduced growth ofEscherichia coliandStaphylococcus aureusfor 4-6 orders of magnitude. Therefore, the 3D-printed Ti-based scaffolds coated with BAGs and (ZnO-)AgHAP have great potential for application as a multifunctional implant with antibacterial properties for the restoration of defects in load-bearing bones.",
publisher = "IOP Publishing",
journal = "Biomedical Materials",
title = "Enhanced antimicrobial properties and bioactivity of 3D-printed titanium scaffolds by multilayer bioceramic coating for large bone defects",
number = "6",
pages = "065020",
volume = "18",
doi = "10.1088/1748-605X/ad02d2"
}

Milivojević, M., Chen, K., Radovanović, Ž., Petrović, R., Dimitrijević-Branković, S., Kojić, V., Marković, D.,& Janaćković, Đ.. (2023). Enhanced antimicrobial properties and bioactivity of 3D-printed titanium scaffolds by multilayer bioceramic coating for large bone defects. in Biomedical Materials
IOP Publishing., 18(6), 065020.
https://doi.org/10.1088/1748-605X/ad02d2

Milivojević M, Chen K, Radovanović Ž, Petrović R, Dimitrijević-Branković S, Kojić V, Marković D, Janaćković Đ. Enhanced antimicrobial properties and bioactivity of 3D-printed titanium scaffolds by multilayer bioceramic coating for large bone defects. in Biomedical Materials. 2023;18(6):065020.
doi:10.1088/1748-605X/ad02d2 .

Milivojević, Marija, Chen, Ke, Radovanović, Željko, Petrović, Rada, Dimitrijević-Branković, Suzana, Kojić, Vesna, Marković, Danica, Janaćković, Đorđe, "Enhanced antimicrobial properties and bioactivity of 3D-printed titanium scaffolds by multilayer bioceramic coating for large bone defects" in Biomedical Materials, 18, no. 6 (2023):065020,
https://doi.org/10.1088/1748-605X/ad02d2 . .

TY  - CONF
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7021
AB  - The use of edible films and coatings made of natural materials such as
polysaccharides provides a sustainable technological alternative to traditional plastic food
packaging materials as one of the contemporary methods for preserving food items and
guaranteeing their quality and freshness throughout their shelf lives. This is especially
important for fresh fruits as highly perishable.
The aim of the present study was to investigate the effect of dextran-based coating on
strawberry (Fragaria × ananassa) quality attributes during refrigerated storage. Dextran was
synthesized using dextransucrase from Leuconostoc mesenteroides T3. Coating solution was
made by dissolving 12g of dextran in 300 mL distilled water. Glycerol (25% w/w) and Tween
80 (5% w/w) were added as plasticizer and surfactant, respectively. Strawberries were coated
by dipping in coating solution or water (control) and after drying were placed in plastic trays
and stored at 8 °C. The influence of coating on strawberry quality was assessed through
measuring the percentage of weight loss (WL), total soluble solids (TSS) and titratable
acidity (TA) of the coated fruits and compared with the control uncoated samples.
The percentage of WL increased during storage in both coated and uncoated
strawberries and although it was less for the uncoated strawberries, the difference was not
significant. The TSS content was lower in dextran-coated fruit during the entire storage
period. Since TSS is an indicator of the ripeness of the fruit it could be concluded that dextran
coating slows down the ripening, thus increasing the shelf life. Unexpectedly, coated
strawberries exhibited lower TA values. However, the relative changes were significantly
smaller among the coated samples group, implying that the coating slows down the
respiration process in which the acids are consumed.
Overall, the results of this work show that the dextran coating has the potential to
preserve quality of strawberries during cold storage.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7021
ER  - 

@conference{
author = "Davidović, Slađana and Miljković, Miona and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "The use of edible films and coatings made of natural materials such as
polysaccharides provides a sustainable technological alternative to traditional plastic food
packaging materials as one of the contemporary methods for preserving food items and
guaranteeing their quality and freshness throughout their shelf lives. This is especially
important for fresh fruits as highly perishable.
The aim of the present study was to investigate the effect of dextran-based coating on
strawberry (Fragaria × ananassa) quality attributes during refrigerated storage. Dextran was
synthesized using dextransucrase from Leuconostoc mesenteroides T3. Coating solution was
made by dissolving 12g of dextran in 300 mL distilled water. Glycerol (25% w/w) and Tween
80 (5% w/w) were added as plasticizer and surfactant, respectively. Strawberries were coated
by dipping in coating solution or water (control) and after drying were placed in plastic trays
and stored at 8 °C. The influence of coating on strawberry quality was assessed through
measuring the percentage of weight loss (WL), total soluble solids (TSS) and titratable
acidity (TA) of the coated fruits and compared with the control uncoated samples.
The percentage of WL increased during storage in both coated and uncoated
strawberries and although it was less for the uncoated strawberries, the difference was not
significant. The TSS content was lower in dextran-coated fruit during the entire storage
period. Since TSS is an indicator of the ripeness of the fruit it could be concluded that dextran
coating slows down the ripening, thus increasing the shelf life. Unexpectedly, coated
strawberries exhibited lower TA values. However, the relative changes were significantly
smaller among the coated samples group, implying that the coating slows down the
respiration process in which the acids are consumed.
Overall, the results of this work show that the dextran coating has the potential to
preserve quality of strawberries during cold storage.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE",
pages = "83",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7021"
}

Davidović, S., Miljković, M.,& Dimitrijević-Branković, S.. (2023). EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 83.
https://hdl.handle.net/21.15107/rcub_technorep_7021

Davidović S, Miljković M, Dimitrijević-Branković S. EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:83.
https://hdl.handle.net/21.15107/rcub_technorep_7021 .

Davidović, Slađana, Miljković, Miona, Dimitrijević-Branković, Suzana, "EFFECT OF DEXTRAN COATING ON STRAWBERRY QUALITY DURING REFRIGERATED STORAGE" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):83,
https://hdl.handle.net/21.15107/rcub_technorep_7021 .

TY  - CONF
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Ilić, Nevena
AU  - Milošević, Jelena
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7030
AB  - By-products of soybean oil production are high-quality protein supplements for
animal feed. However, they are rich in anti-nutritive factors and indigestible components,
among which the special attention is focused on galactooligosaccharides, due to lack of αgalactosidase in monogastric animals. Enzymatic treatment of soy-based products and
microbial fermentation are employed for overcoming these issues.
The main goal of this study was to apply fermentation with a selected strain of black
yeast-like fungus (Aureobasidium spp.) of the soybean products in order to obtain highprotein soy-based animal feed. First, screening for an appropriate strain of microorganism
among ten natural isolates from grapes has been performed. Keeping in mind complex
structure of soybean polysaccharides the first selection criterion was the ability of growth in
the presence of arabinose, xylose, galactose, mannose, raffinose, or soybean oil as sole
carbon source. More important, enzymatic activity of α-galactosidase was detected in new
isolates. The highest α-galactosidase producer was selected for cultivation on soybean meal.
The selected strain was characterized in terms of physiological and technological properties
and identified as Aureobasidium pullulans P8. In order to obtain high protein soybean meal, it
has been utilized in two types of fermentation, i.e. solid state (SSF) and submerged (SMF)
under varying conditions. Maximal protein content (61.11% based on dry weight) was
obtained after 5 days of SMF at 30 °C and 10% of dry matter, while SSF produced 57.78%
protein after 7 days of incubation at 30 °C with substrate contained 30% of dry matter.
Extracellular enzymatic activities of cellulase, pectinase, amylase, xylanase and αgalactosidase were detected in supernatant after SMF, indicating its potential reusability for
hydrolysis of new batch of soybean substrate.
This investigation revealed the versatile extracellular enzymatic potential of newly
isolated black yeast-like fungus Aureobasidium pullulans P8 and its potential for production
of high protein soybean meal.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023
T1  - VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7030
ER  - 

@conference{
author = "Davidović, Slađana and Miljković, Miona and Ilić, Nevena and Milošević, Jelena and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "By-products of soybean oil production are high-quality protein supplements for
animal feed. However, they are rich in anti-nutritive factors and indigestible components,
among which the special attention is focused on galactooligosaccharides, due to lack of αgalactosidase in monogastric animals. Enzymatic treatment of soy-based products and
microbial fermentation are employed for overcoming these issues.
The main goal of this study was to apply fermentation with a selected strain of black
yeast-like fungus (Aureobasidium spp.) of the soybean products in order to obtain highprotein soy-based animal feed. First, screening for an appropriate strain of microorganism
among ten natural isolates from grapes has been performed. Keeping in mind complex
structure of soybean polysaccharides the first selection criterion was the ability of growth in
the presence of arabinose, xylose, galactose, mannose, raffinose, or soybean oil as sole
carbon source. More important, enzymatic activity of α-galactosidase was detected in new
isolates. The highest α-galactosidase producer was selected for cultivation on soybean meal.
The selected strain was characterized in terms of physiological and technological properties
and identified as Aureobasidium pullulans P8. In order to obtain high protein soybean meal, it
has been utilized in two types of fermentation, i.e. solid state (SSF) and submerged (SMF)
under varying conditions. Maximal protein content (61.11% based on dry weight) was
obtained after 5 days of SMF at 30 °C and 10% of dry matter, while SSF produced 57.78%
protein after 7 days of incubation at 30 °C with substrate contained 30% of dry matter.
Extracellular enzymatic activities of cellulase, pectinase, amylase, xylanase and αgalactosidase were detected in supernatant after SMF, indicating its potential reusability for
hydrolysis of new batch of soybean substrate.
This investigation revealed the versatile extracellular enzymatic potential of newly
isolated black yeast-like fungus Aureobasidium pullulans P8 and its potential for production
of high protein soybean meal.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023",
title = "VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS",
pages = "84",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7030"
}

Davidović, S., Miljković, M., Ilić, N., Milošević, J.,& Dimitrijević-Branković, S.. (2023). VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023
Belgrade : University, Faculty of Technology and Metallurgy., 84.
https://hdl.handle.net/21.15107/rcub_technorep_7030

Davidović S, Miljković M, Ilić N, Milošević J, Dimitrijević-Branković S. VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023. 2023;:84.
https://hdl.handle.net/21.15107/rcub_technorep_7030 .

Davidović, Slađana, Miljković, Miona, Ilić, Nevena, Milošević, Jelena, Dimitrijević-Branković, Suzana, "VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023 (2023):84,
https://hdl.handle.net/21.15107/rcub_technorep_7030 .

TY  - JOUR
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Milić, Marija
AU  - Rajilić-Stojanović, Mirjana
AU  - Pecarski, Danijela
AU  - Ivancic-Santek, Mirela
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5001
AB  - This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewer's spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewer 's spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewer's spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.
T2  - Biomass Conversion and Biorefinery
T1  - Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production
EP  - 17186
SP  - 17175
VL  - 13
DO  - 10.1007/s13399-021-02145-x
ER  - 

@article{
author = "Ilić, Nevena and Davidović, Slađana and Milić, Marija and Rajilić-Stojanović, Mirjana and Pecarski, Danijela and Ivancic-Santek, Mirela and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewer's spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewer 's spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewer's spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.",
journal = "Biomass Conversion and Biorefinery",
title = "Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production",
pages = "17186-17175",
volume = "13",
doi = "10.1007/s13399-021-02145-x"
}

Ilić, N., Davidović, S., Milić, M., Rajilić-Stojanović, M., Pecarski, D., Ivancic-Santek, M., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2022). Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. in Biomass Conversion and Biorefinery, 13, 17175-17186.
https://doi.org/10.1007/s13399-021-02145-x

Ilić N, Davidović S, Milić M, Rajilić-Stojanović M, Pecarski D, Ivancic-Santek M, Mihajlovski K, Dimitrijević-Branković S. Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. in Biomass Conversion and Biorefinery. 2022;13:17175-17186.
doi:10.1007/s13399-021-02145-x .

Ilić, Nevena, Davidović, Slađana, Milić, Marija, Rajilić-Stojanović, Mirjana, Pecarski, Danijela, Ivancic-Santek, Mirela, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production" in Biomass Conversion and Biorefinery, 13 (2022):17175-17186,
https://doi.org/10.1007/s13399-021-02145-x . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Kostić, Anđela
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5940
AB  - In recent years, one of the biggest environmental problems is the pollution of water with colored wastewater which has negative effect on the environment and human health. Wastewaters contain complex structural compounds, such as azo dyes that used today in many industrial fields. Removing of azo dyes from wastewater using traditional methods is an extremely complex and in many cases ineffective process. In recent decades, there is a tendency towards the application of environmentally acceptable methods of removing synthetic dyes from wastewater. Method which has proven to be very effective, is the degradation of synthetic dyes using various fungal enzymes. In this study, the crude fungal laccase (31,42 UmL-1) obtained by growing fungal mycelium Ganoderma spp. on cereal mix was used for decolorization of Methyl Orange. Decolorization procedure was carried out at different temperatures (30-70 ºC) and pH (3-8) in order to determine the optimal conditions for dye decolorization. The incubation time was 180 min and every 15 min during the incubation time, the change in color intensity was monitored spectrophotometrically at 472 nm and decolorization efficiency (DE) was calculated. The optimal pH was 5 with DE of 57 % at 30 ºC, while in the case of other pH values, DE was lower. The lowest DE (1,2 %) was in the case of pH 8, which indicates that laccase activity decreases in the alkaline medium. The optimal temperature of decolorization was 50 ºC with DE of 62 % at pH 5, while the DE was lower at higher and lower temperatures, which is in accordance with the literature data on the laccase activity optimal temperature of the Ganoderma spp. The lowest DE was 35 % at 70 ºC and pH 5. The obtained results show that laccase with good decolorization properties can be obtained using cheap agro-industrial wastes, such as cereal mix. The low cost of laccase production as well as the relatively high DE in a short time may further broaden its application in wastewater treatment.
C3  - Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,
T1  - Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5940
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Kostić, Anđela and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "In recent years, one of the biggest environmental problems is the pollution of water with colored wastewater which has negative effect on the environment and human health. Wastewaters contain complex structural compounds, such as azo dyes that used today in many industrial fields. Removing of azo dyes from wastewater using traditional methods is an extremely complex and in many cases ineffective process. In recent decades, there is a tendency towards the application of environmentally acceptable methods of removing synthetic dyes from wastewater. Method which has proven to be very effective, is the degradation of synthetic dyes using various fungal enzymes. In this study, the crude fungal laccase (31,42 UmL-1) obtained by growing fungal mycelium Ganoderma spp. on cereal mix was used for decolorization of Methyl Orange. Decolorization procedure was carried out at different temperatures (30-70 ºC) and pH (3-8) in order to determine the optimal conditions for dye decolorization. The incubation time was 180 min and every 15 min during the incubation time, the change in color intensity was monitored spectrophotometrically at 472 nm and decolorization efficiency (DE) was calculated. The optimal pH was 5 with DE of 57 % at 30 ºC, while in the case of other pH values, DE was lower. The lowest DE (1,2 %) was in the case of pH 8, which indicates that laccase activity decreases in the alkaline medium. The optimal temperature of decolorization was 50 ºC with DE of 62 % at pH 5, while the DE was lower at higher and lower temperatures, which is in accordance with the literature data on the laccase activity optimal temperature of the Ganoderma spp. The lowest DE was 35 % at 70 ºC and pH 5. The obtained results show that laccase with good decolorization properties can be obtained using cheap agro-industrial wastes, such as cereal mix. The low cost of laccase production as well as the relatively high DE in a short time may further broaden its application in wastewater treatment.",
journal = "Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,",
title = "Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5940"
}

Ilić, N., Milić, M., Davidović, S., Kostić, A., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2022). Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix. in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,, 80.
https://hdl.handle.net/21.15107/rcub_technorep_5940

Ilić N, Milić M, Davidović S, Kostić A, Mihajlovski K, Dimitrijević-Branković S. Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix. in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,. 2022;:80.
https://hdl.handle.net/21.15107/rcub_technorep_5940 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Kostić, Anđela, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix" in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering, (2022):80,
https://hdl.handle.net/21.15107/rcub_technorep_5940 .

TY  - JOUR
AU  - Dimitrijević, Snežana
AU  - Filipović, Vladimir
AU  - Milić, Marija
AU  - Dimitrijević-Branković, Suzana
AU  - Ugrenović, Vladan
AU  - Popović, Vera
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6412
AB  - U cilju proučavanja uticaja bakterija koje stimulišu rast biljaka, a koje pripadaju rodu Strep-
tomices sp , Paenibacillus sp i Hymenibacter sp , na prinos i kvalitet semena uljanog lana i crnog
kima, kao i na morfološke karakteristike biljaka, sprovedeni su dvogodišnji terenski eksperimen-
ti Tretman zemljišta mešanom kulturom bakterija različitih koncentracija je primenjen tokom
setve biljaka Pre početka setve ispitan je uticaj pojedinačnih sojeva na indeks klijavosti seme-
na uljanog lana i crnog kima Na osnovu rezultata prikazanih u radu, može se uočiti da sojevi,
CKS1 – Paenybacillus chitinolyticus, CKS3 – Hymenobacter sp , CKS7 – Streptomyces fulvissimus,
u svim ispitivanim koncentracijama, daju vrednosti indeksa klijavosti GI preko 100% kod uljanog
lana, dok je kod crnog kima takav efekat pokazao samo soj CKS7 Pozitivno dejstvo bakterijskog
preparata kod obe ispitane biljne vrste zabeleženo je merenjem prinosa semena po hektaru tre-
tiranom manjom koncentracijom bakterijskog preparata, u odnosu na odgovarajuće netretirane
uzorke (kontrolu) Pozitivna razlika zabeležena je merenjem broja čaura u uzorcima tretiranog
uljanog lana i crnog kima u odnosu na netretirani, kao i mase korena obe tretirane biljne vrste
Takođe, najveću ukupnu klijavost u odnosu na kontrolu je ostvarila varijanta uljanog lana L2 u
tretmanu manjom koncentracijom bakterija od 98,3±1,4%.
PB  - Serbian Association of Plant Breedings and Seed Producers, Belgrade
T2  - Selekcija i semenarstvo
T1  - Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima
EP  - 38
IS  - 2
SP  - 30
VL  - 28
DO  - 10.5937/SelSem2202030D
ER  - 

@article{
author = "Dimitrijević, Snežana and Filipović, Vladimir and Milić, Marija and Dimitrijević-Branković, Suzana and Ugrenović, Vladan and Popović, Vera",
year = "2022",
abstract = "U cilju proučavanja uticaja bakterija koje stimulišu rast biljaka, a koje pripadaju rodu Strep-
tomices sp , Paenibacillus sp i Hymenibacter sp , na prinos i kvalitet semena uljanog lana i crnog
kima, kao i na morfološke karakteristike biljaka, sprovedeni su dvogodišnji terenski eksperimen-
ti Tretman zemljišta mešanom kulturom bakterija različitih koncentracija je primenjen tokom
setve biljaka Pre početka setve ispitan je uticaj pojedinačnih sojeva na indeks klijavosti seme-
na uljanog lana i crnog kima Na osnovu rezultata prikazanih u radu, može se uočiti da sojevi,
CKS1 – Paenybacillus chitinolyticus, CKS3 – Hymenobacter sp , CKS7 – Streptomyces fulvissimus,
u svim ispitivanim koncentracijama, daju vrednosti indeksa klijavosti GI preko 100% kod uljanog
lana, dok je kod crnog kima takav efekat pokazao samo soj CKS7 Pozitivno dejstvo bakterijskog
preparata kod obe ispitane biljne vrste zabeleženo je merenjem prinosa semena po hektaru tre-
tiranom manjom koncentracijom bakterijskog preparata, u odnosu na odgovarajuće netretirane
uzorke (kontrolu) Pozitivna razlika zabeležena je merenjem broja čaura u uzorcima tretiranog
uljanog lana i crnog kima u odnosu na netretirani, kao i mase korena obe tretirane biljne vrste
Takođe, najveću ukupnu klijavost u odnosu na kontrolu je ostvarila varijanta uljanog lana L2 u
tretmanu manjom koncentracijom bakterija od 98,3±1,4%.",
publisher = "Serbian Association of Plant Breedings and Seed Producers, Belgrade",
journal = "Selekcija i semenarstvo",
title = "Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima",
pages = "38-30",
number = "2",
volume = "28",
doi = "10.5937/SelSem2202030D"
}

Dimitrijević, S., Filipović, V., Milić, M., Dimitrijević-Branković, S., Ugrenović, V.,& Popović, V.. (2022). Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima. in Selekcija i semenarstvo
Serbian Association of Plant Breedings and Seed Producers, Belgrade., 28(2), 30-38.
https://doi.org/10.5937/SelSem2202030D

Dimitrijević S, Filipović V, Milić M, Dimitrijević-Branković S, Ugrenović V, Popović V. Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima. in Selekcija i semenarstvo. 2022;28(2):30-38.
doi:10.5937/SelSem2202030D .

Dimitrijević, Snežana, Filipović, Vladimir, Milić, Marija, Dimitrijević-Branković, Suzana, Ugrenović, Vladan, Popović, Vera, "Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima" in Selekcija i semenarstvo, 28, no. 2 (2022):30-38,
https://doi.org/10.5937/SelSem2202030D . .

TY  - CONF
AU  - Vasić, Katja
AU  - Ilić, Nevena
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
AU  - Knez, Željko
AU  - Leitgeb, Maja
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5941
AB  - The principles of green chemistry encourage simple and eco-friendly approaches,
using various enzymes for different industries, such as fine chemicals productions
and water treatments. To increase operational stability of enzymes, different immobilization techniques must be employed in preparation of highly active
biocatalysts. Entrapment of enzymes within a polymer matrix is the simplest immobilization method. Properties, such as size
of beads, porosity, enzyme leakage degree,
and stability are optimized through alginate and calcium concentrations and the
use of cross-linking agents.
Alginate beads were prepared in calcium chloride and later on activated using
cross-linking agent glutaraldehyde (GA),
where GA concentration and time of activation were optimized. Such GA-activated
alginate beads were further immobilized
with laccase, where the time of immobilization was optimized. Immobilization efficiency and residual activity were determined after optimization protocol,
resulting in 97 % and 88 %, respectively.
Results indicate that laccase can be immobilized onto alginate beads and their residual activity can be improved by varying
the GA concentration for beads’ activation. Such GA-activated alginate beads can
be successfully used for laccase immobilization for further applications.
PB  - Wiley
C3  - Book of Abstracts- Chemie Ingenieur Technik
T1  - Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde
IS  - 9
SP  - 1266
VL  - 94
DO  - 10.1002/cite.202255288
ER  - 

@conference{
author = "Vasić, Katja and Ilić, Nevena and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana and Knez, Željko and Leitgeb, Maja",
year = "2022",
abstract = "The principles of green chemistry encourage simple and eco-friendly approaches,
using various enzymes for different industries, such as fine chemicals productions
and water treatments. To increase operational stability of enzymes, different immobilization techniques must be employed in preparation of highly active
biocatalysts. Entrapment of enzymes within a polymer matrix is the simplest immobilization method. Properties, such as size
of beads, porosity, enzyme leakage degree,
and stability are optimized through alginate and calcium concentrations and the
use of cross-linking agents.
Alginate beads were prepared in calcium chloride and later on activated using
cross-linking agent glutaraldehyde (GA),
where GA concentration and time of activation were optimized. Such GA-activated
alginate beads were further immobilized
with laccase, where the time of immobilization was optimized. Immobilization efficiency and residual activity were determined after optimization protocol,
resulting in 97 % and 88 %, respectively.
Results indicate that laccase can be immobilized onto alginate beads and their residual activity can be improved by varying
the GA concentration for beads’ activation. Such GA-activated alginate beads can
be successfully used for laccase immobilization for further applications.",
publisher = "Wiley",
journal = "Book of Abstracts- Chemie Ingenieur Technik",
title = "Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde",
number = "9",
pages = "1266",
volume = "94",
doi = "10.1002/cite.202255288"
}

Vasić, K., Ilić, N., Mihajlovski, K., Dimitrijević-Branković, S., Knez, Ž.,& Leitgeb, M.. (2022). Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde. in Book of Abstracts- Chemie Ingenieur Technik
Wiley., 94(9), 1266.
https://doi.org/10.1002/cite.202255288

Vasić K, Ilić N, Mihajlovski K, Dimitrijević-Branković S, Knez Ž, Leitgeb M. Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde. in Book of Abstracts- Chemie Ingenieur Technik. 2022;94(9):1266.
doi:10.1002/cite.202255288 .

Vasić, Katja, Ilić, Nevena, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, Knez, Željko, Leitgeb, Maja, "Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde" in Book of Abstracts- Chemie Ingenieur Technik, 94, no. 9 (2022):1266,
https://doi.org/10.1002/cite.202255288 . .

TY  - JOUR
AU  - Jovanović, Aleksandra A.
AU  - Vajić, Una-Jovana V.
AU  - Mijin, Dušan Ž.
AU  - Zdunić, Gordana M.
AU  - Šavikin, Katarina P.
AU  - Branković, Suzana
AU  - Kitić, Dušanka
AU  - Bugarski, Branko M.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5194
AB  - In the present study, polyphenol extraction from Thymus serpyllum by-product (herbal dust) in microwave reactor was optimized through varying extraction time, ethanol concentration and solid-to-solvent ratiovia determination of total polyphenol and flavonoid contents, and antioxidant activity, using response surface methodology and a central composite design. Total polyphenol and flavonoids contents, as well as antioxidant activity of the extracts were statistically significantly affected by ethanol concentration and solid-to-solvent ratio. However, extraction time had statistically significant influence only on polyphenol yield. The extraction conditions that maximized polyphenol recovery were 48% ethanol and 0.0402 g/mL solid-to-solvent ratio during 86 s of microwave-assisted extraction, and under these conditions total polyphenol content was estimated to be 57.2 mg of gallic acid equivalents (GAE)/L, while measured value was 58.1 ± 3.2 mg GAE/L. Polyphenol content (HPLC analysis), antioxidant, antimicrobial and antispasmodic potential of the extract prepared under these optimal extraction conditions were evaluated. Rosmarinic acids, luteolin 7-O-glucuronide, caffeic acid, apigenin glucuronide, 6-hydroxyluteolin 7-O-glucoside, 6,8-di-C-glucosylapigenin, and traces of chlorogenic acid were quantified in the extract (descending order of content). The selected extract inhibited 95% ± 0.9 of lipid peroxidation in β-Carotene and linoleic acid emulsion. It was more effective against tested Gram-positive bacteria than against Gram-negative and showed dose-dependent antispasmodic activity on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions. Microwave-assisted extraction could be selected as a successful technique for polyphenol extraction from wild thyme by-product. Biological activities shown in the study open the possibility of potential application of the extract in pharmaceutical, food, functional food and cosmetic products
PB  - Elsevier GmbH
T2  - Journal of Applied Research on Medicinal and Aromatic Plants
T1  - Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract
SP  - 100417
VL  - 31
DO  - 10.1016/j.jarmap.2022.100417
ER  - 

@article{
author = "Jovanović, Aleksandra A. and Vajić, Una-Jovana V. and Mijin, Dušan Ž. and Zdunić, Gordana M. and Šavikin, Katarina P. and Branković, Suzana and Kitić, Dušanka and Bugarski, Branko M.",
year = "2022",
abstract = "In the present study, polyphenol extraction from Thymus serpyllum by-product (herbal dust) in microwave reactor was optimized through varying extraction time, ethanol concentration and solid-to-solvent ratiovia determination of total polyphenol and flavonoid contents, and antioxidant activity, using response surface methodology and a central composite design. Total polyphenol and flavonoids contents, as well as antioxidant activity of the extracts were statistically significantly affected by ethanol concentration and solid-to-solvent ratio. However, extraction time had statistically significant influence only on polyphenol yield. The extraction conditions that maximized polyphenol recovery were 48% ethanol and 0.0402 g/mL solid-to-solvent ratio during 86 s of microwave-assisted extraction, and under these conditions total polyphenol content was estimated to be 57.2 mg of gallic acid equivalents (GAE)/L, while measured value was 58.1 ± 3.2 mg GAE/L. Polyphenol content (HPLC analysis), antioxidant, antimicrobial and antispasmodic potential of the extract prepared under these optimal extraction conditions were evaluated. Rosmarinic acids, luteolin 7-O-glucuronide, caffeic acid, apigenin glucuronide, 6-hydroxyluteolin 7-O-glucoside, 6,8-di-C-glucosylapigenin, and traces of chlorogenic acid were quantified in the extract (descending order of content). The selected extract inhibited 95% ± 0.9 of lipid peroxidation in β-Carotene and linoleic acid emulsion. It was more effective against tested Gram-positive bacteria than against Gram-negative and showed dose-dependent antispasmodic activity on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions. Microwave-assisted extraction could be selected as a successful technique for polyphenol extraction from wild thyme by-product. Biological activities shown in the study open the possibility of potential application of the extract in pharmaceutical, food, functional food and cosmetic products",
publisher = "Elsevier GmbH",
journal = "Journal of Applied Research on Medicinal and Aromatic Plants",
title = "Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract",
pages = "100417",
volume = "31",
doi = "10.1016/j.jarmap.2022.100417"
}

Jovanović, A. A., Vajić, U. V., Mijin, D. Ž., Zdunić, G. M., Šavikin, K. P., Branković, S., Kitić, D.,& Bugarski, B. M.. (2022). Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract. in Journal of Applied Research on Medicinal and Aromatic Plants
Elsevier GmbH., 31, 100417.
https://doi.org/10.1016/j.jarmap.2022.100417

Jovanović AA, Vajić UV, Mijin DŽ, Zdunić GM, Šavikin KP, Branković S, Kitić D, Bugarski BM. Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract. in Journal of Applied Research on Medicinal and Aromatic Plants. 2022;31:100417.
doi:10.1016/j.jarmap.2022.100417 .

Jovanović, Aleksandra A., Vajić, Una-Jovana V., Mijin, Dušan Ž., Zdunić, Gordana M., Šavikin, Katarina P., Branković, Suzana, Kitić, Dušanka, Bugarski, Branko M., "Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract" in Journal of Applied Research on Medicinal and Aromatic Plants, 31 (2022):100417,
https://doi.org/10.1016/j.jarmap.2022.100417 . .

TY  - JOUR
AU  - Milutinović, Milica
AU  - Miladinović, Marija
AU  - Gašić, Uroš
AU  - Dimitrijević-Branković, Suzana
AU  - Rajilić‑Stojanović, Mirjana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5137
AB  - Microwave-assisted extraction (MAE) conditions were optimized to improve extract quality of medicinal herb — Hypericum perforatum L. (St. John’s wort) dust. Response surface methodology was applied initially to obtain the highest concentration of total polyphenols in extract solids (MAE-e). St. John’s wort was mixed with 30% ethanol in 50 mL/g solvent to solid ratio, and treated with 170 W microwave power for 40 s to yield an extract with 411.26 ± 6.21 mg GAE/g of polyphenols. This extract contained a significantly higher content of polyphenols (42.50%) and had significantly higher antioxidant activity than the macerate obtained by using European Medicines Agency (EMA) recommended procedure. The advantage of the EMA procedure was the higher yield of extract per gram of plant material. Therefore, another set of MAE conditions was defined to maximize the yield of polyphenols per gram of plant material (MAE-p). The MAE-p extract was produced by using 30% ethanol, 10 mL/g solvent to solid ratio, and 170 W microwave power for 100 s, which was, again, a markedly shorter period than 72 h of maceration. The MAE-p extract had a slightly, but significantly higher yield (5.2%), more polyphenols (8.8%), and improved antioxidant activity compared to the EMA macerate. Antimicrobial activity against several pathogens was stronger for the MAE extracts. The chemical composition of extracts was slightly different and MAE favored extraction of glycosides, in particular, rutin (quercetin-3-O-rutinoside), while the EMA macerate contained quercetin aglycon in the highest concentration. Our study demonstrates that statistically planned experiments allow for significant improvement of the extraction process, which application could facilitate better use of natural resources and deliver more potent extracts than those obtained by currently recommended procedures.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Biomass Conversion and Biorefinery
T1  - Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction
DO  - 10.1007/s13399-022-02717-5
ER  - 

@article{
author = "Milutinović, Milica and Miladinović, Marija and Gašić, Uroš and Dimitrijević-Branković, Suzana and Rajilić‑Stojanović, Mirjana",
year = "2022",
abstract = "Microwave-assisted extraction (MAE) conditions were optimized to improve extract quality of medicinal herb — Hypericum perforatum L. (St. John’s wort) dust. Response surface methodology was applied initially to obtain the highest concentration of total polyphenols in extract solids (MAE-e). St. John’s wort was mixed with 30% ethanol in 50 mL/g solvent to solid ratio, and treated with 170 W microwave power for 40 s to yield an extract with 411.26 ± 6.21 mg GAE/g of polyphenols. This extract contained a significantly higher content of polyphenols (42.50%) and had significantly higher antioxidant activity than the macerate obtained by using European Medicines Agency (EMA) recommended procedure. The advantage of the EMA procedure was the higher yield of extract per gram of plant material. Therefore, another set of MAE conditions was defined to maximize the yield of polyphenols per gram of plant material (MAE-p). The MAE-p extract was produced by using 30% ethanol, 10 mL/g solvent to solid ratio, and 170 W microwave power for 100 s, which was, again, a markedly shorter period than 72 h of maceration. The MAE-p extract had a slightly, but significantly higher yield (5.2%), more polyphenols (8.8%), and improved antioxidant activity compared to the EMA macerate. Antimicrobial activity against several pathogens was stronger for the MAE extracts. The chemical composition of extracts was slightly different and MAE favored extraction of glycosides, in particular, rutin (quercetin-3-O-rutinoside), while the EMA macerate contained quercetin aglycon in the highest concentration. Our study demonstrates that statistically planned experiments allow for significant improvement of the extraction process, which application could facilitate better use of natural resources and deliver more potent extracts than those obtained by currently recommended procedures.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Biomass Conversion and Biorefinery",
title = "Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction",
doi = "10.1007/s13399-022-02717-5"
}

Milutinović, M., Miladinović, M., Gašić, U., Dimitrijević-Branković, S.,& Rajilić‑Stojanović, M.. (2022). Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction. in Biomass Conversion and Biorefinery
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s13399-022-02717-5

Milutinović M, Miladinović M, Gašić U, Dimitrijević-Branković S, Rajilić‑Stojanović M. Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction. in Biomass Conversion and Biorefinery. 2022;.
doi:10.1007/s13399-022-02717-5 .

Milutinović, Milica, Miladinović, Marija, Gašić, Uroš, Dimitrijević-Branković, Suzana, Rajilić‑Stojanović, Mirjana, "Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction" in Biomass Conversion and Biorefinery (2022),
https://doi.org/10.1007/s13399-022-02717-5 . .

TY  - PAT
AU  - Simović, Milica
AU  - Bezbradica, Dejan
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5990
AB  - Predmetni pronalazak se odnosi na "in situ" enzimski
postupak sinteze frukto-oligosaharida direktnom
transfruktozilacijom saharoze prisutne u prehrambenim
proizvodima korišćenjem enzima fruktozil-transferaze. Na taj
način sharoza prisutna u proizvodu se konvertuje u
prebiotike, frukto-oligosaharide, pri čemu se dobija proizvod
poboljšanih funkcionalnih svojstava, sa smanjenim sadržajem
saharoze, tj. manjim glikemijskim indeksom. Takođe,
predmetni postupak se odnosi i na proizvode sa prebiotskom
aktivnošću proizvedene prema metodama ovog pronalaska
PB  - Гласник интелектуалне својине
T2  - Glasnik intelektualne svojine
T1  - Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics
IS  - 3
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5990
ER  - 

@misc{
author = "Simović, Milica and Bezbradica, Dejan and Ćorović, Marija and Milivojević, Ana and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "Predmetni pronalazak se odnosi na "in situ" enzimski
postupak sinteze frukto-oligosaharida direktnom
transfruktozilacijom saharoze prisutne u prehrambenim
proizvodima korišćenjem enzima fruktozil-transferaze. Na taj
način sharoza prisutna u proizvodu se konvertuje u
prebiotike, frukto-oligosaharide, pri čemu se dobija proizvod
poboljšanih funkcionalnih svojstava, sa smanjenim sadržajem
saharoze, tj. manjim glikemijskim indeksom. Takođe,
predmetni postupak se odnosi i na proizvode sa prebiotskom
aktivnošću proizvedene prema metodama ovog pronalaska",
publisher = "Гласник интелектуалне својине",
journal = "Glasnik intelektualne svojine",
title = "Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics",
number = "3",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5990"
}

Simović, M., Bezbradica, D., Ćorović, M., Milivojević, A.,& Dimitrijević-Branković, S.. (2022). Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics. in Glasnik intelektualne svojine
Гласник интелектуалне својине.(3), 8.
https://hdl.handle.net/21.15107/rcub_technorep_5990

Simović M, Bezbradica D, Ćorović M, Milivojević A, Dimitrijević-Branković S. Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics. in Glasnik intelektualne svojine. 2022;(3):8.
https://hdl.handle.net/21.15107/rcub_technorep_5990 .

Simović, Milica, Bezbradica, Dejan, Ćorović, Marija, Milivojević, Ana, Dimitrijević-Branković, Suzana, "Food products modification by direct enzymatic synthesis of fructo-oligosaccharides with purpose of reduction of sucrose content and enrichment of the products with prebiotics" in Glasnik intelektualne svojine, no. 3 (2022):8,
https://hdl.handle.net/21.15107/rcub_technorep_5990 .

TY  - JOUR
AU  - Pajnik, Jelena
AU  - Milovanović, Stoja
AU  - Stojanović, Dušica
AU  - Dimitrijević-Branković, Suzana
AU  - Janković-Častvan, Ivona
AU  - Uskoković, Petar
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4979
AB  - Outbreak of surgical infections and bacterial resistance against synthetic antibiotic has prompted research towards development of antibacterial medical devices. For that purpose, absorbable surgical sutures were impregnated with natural compound thymol using an environmentally friendly medium. The supercritical solvent impregnation process was performed at 35 degrees C and 10 MPa during 1, 2, 3 and 6 h, resulting in thymol loadings of 3.9-5.6%. All tested samples expressed antibacterial activity against E. coli and S. aureus. Release tests in PBS at 37 degrees C showed that impregnated material released loaded thymol in a gradual manner. While sutures impregnated during 6 h released complete amount of loaded thymol during 6 days, shorter impregnation process resulted in sutures that retained significant thymol amounts (similar to 20-40%) after 10 days of release test. The effect of proposed process on final material properties and its application were explained in detail using characterization techniques SEM, FTIR, DSC and DMA.
PB  - Elsevier B.V.
T2  - Journal of Supercritical Fluids
T1  - Utilization of supercritical carbon dioxide for development of antibacterial surgical sutures
SP  - 105490
VL  - 181
DO  - 10.1016/j.supflu.2021.105490
ER  - 

@article{
author = "Pajnik, Jelena and Milovanović, Stoja and Stojanović, Dušica and Dimitrijević-Branković, Suzana and Janković-Častvan, Ivona and Uskoković, Petar",
year = "2022",
abstract = "Outbreak of surgical infections and bacterial resistance against synthetic antibiotic has prompted research towards development of antibacterial medical devices. For that purpose, absorbable surgical sutures were impregnated with natural compound thymol using an environmentally friendly medium. The supercritical solvent impregnation process was performed at 35 degrees C and 10 MPa during 1, 2, 3 and 6 h, resulting in thymol loadings of 3.9-5.6%. All tested samples expressed antibacterial activity against E. coli and S. aureus. Release tests in PBS at 37 degrees C showed that impregnated material released loaded thymol in a gradual manner. While sutures impregnated during 6 h released complete amount of loaded thymol during 6 days, shorter impregnation process resulted in sutures that retained significant thymol amounts (similar to 20-40%) after 10 days of release test. The effect of proposed process on final material properties and its application were explained in detail using characterization techniques SEM, FTIR, DSC and DMA.",
publisher = "Elsevier B.V.",
journal = "Journal of Supercritical Fluids",
title = "Utilization of supercritical carbon dioxide for development of antibacterial surgical sutures",
pages = "105490",
volume = "181",
doi = "10.1016/j.supflu.2021.105490"
}

Pajnik, J., Milovanović, S., Stojanović, D., Dimitrijević-Branković, S., Janković-Častvan, I.,& Uskoković, P.. (2022). Utilization of supercritical carbon dioxide for development of antibacterial surgical sutures. in Journal of Supercritical Fluids
Elsevier B.V.., 181, 105490.
https://doi.org/10.1016/j.supflu.2021.105490

Pajnik J, Milovanović S, Stojanović D, Dimitrijević-Branković S, Janković-Častvan I, Uskoković P. Utilization of supercritical carbon dioxide for development of antibacterial surgical sutures. in Journal of Supercritical Fluids. 2022;181:105490.
doi:10.1016/j.supflu.2021.105490 .

Pajnik, Jelena, Milovanović, Stoja, Stojanović, Dušica, Dimitrijević-Branković, Suzana, Janković-Častvan, Ivona, Uskoković, Petar, "Utilization of supercritical carbon dioxide for development of antibacterial surgical sutures" in Journal of Supercritical Fluids, 181 (2022):105490,
https://doi.org/10.1016/j.supflu.2021.105490 . .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Buntić, Aneta
AU  - Milić, Marija
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/20
AB  - Purpose To avoid a negative environmental and economic impact of agricultural wastes, and following the principles of circular economy, the reuse of agricultural wastes is necessary. For this purpose, isolation of novel microorganisms with potential biotechnological application is recommended. The current researches in bioethanol production are aimed to reduce the production costs using low-cost substrates and in-house produced enzymes by novel isolated microorganisms. In line with this, in this study valorization of these agricultural by-products by novel isolate S. fulvissimus CKS7 to biotechnological value added products was done. Methods Standard microbiological methods were used for the isolation and characterization of strain. Enzymes activities were determinated using DNS method while, the ethanol concentration was determined based on the density of the alcohol distillate at 20 degrees C. Results The maximal enzymatic activities for amylase, cellulases (carboxymethyl cellulase and Avicelase), pectinase and xylanase were achieved using rye bran as a waste substrate for CKS7 growth. Obtained crude bacterial enzymes were used for enzymatic hydrolysis of lignocellulosic materials including horsetail waste, yellow gentian waste, corn stover, cotton material and corona pre-treated cotton material. The maximum yield of reducing sugars was obtained on horsetail waste and corona pre-treated cotton material. Waste brewer's yeast Saccharomyces cerevisiae was successfully used for the production of bioethanol using horsetail waste hydrolysate and corona pre-treated cotton material hydrolysate. Conclusion The obtained results showed that bacterial strain CKS7 has a significant, still unexplored enzymatic potential that could be used to achieve a cleaner, environmental friendly and economically acceptable biofuel production. [GRAPHICS] .
PB  - Springer, Dordrecht
T2  - Waste and Biomass Valorization
T1  - From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production
EP  - 174
IS  - 1
SP  - 165
VL  - 12
DO  - 10.1007/s12649-020-00960-3
ER  - 

@article{
author = "Mihajlovski, Katarina and Buntić, Aneta and Milić, Marija and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "Purpose To avoid a negative environmental and economic impact of agricultural wastes, and following the principles of circular economy, the reuse of agricultural wastes is necessary. For this purpose, isolation of novel microorganisms with potential biotechnological application is recommended. The current researches in bioethanol production are aimed to reduce the production costs using low-cost substrates and in-house produced enzymes by novel isolated microorganisms. In line with this, in this study valorization of these agricultural by-products by novel isolate S. fulvissimus CKS7 to biotechnological value added products was done. Methods Standard microbiological methods were used for the isolation and characterization of strain. Enzymes activities were determinated using DNS method while, the ethanol concentration was determined based on the density of the alcohol distillate at 20 degrees C. Results The maximal enzymatic activities for amylase, cellulases (carboxymethyl cellulase and Avicelase), pectinase and xylanase were achieved using rye bran as a waste substrate for CKS7 growth. Obtained crude bacterial enzymes were used for enzymatic hydrolysis of lignocellulosic materials including horsetail waste, yellow gentian waste, corn stover, cotton material and corona pre-treated cotton material. The maximum yield of reducing sugars was obtained on horsetail waste and corona pre-treated cotton material. Waste brewer's yeast Saccharomyces cerevisiae was successfully used for the production of bioethanol using horsetail waste hydrolysate and corona pre-treated cotton material hydrolysate. Conclusion The obtained results showed that bacterial strain CKS7 has a significant, still unexplored enzymatic potential that could be used to achieve a cleaner, environmental friendly and economically acceptable biofuel production. [GRAPHICS] .",
publisher = "Springer, Dordrecht",
journal = "Waste and Biomass Valorization",
title = "From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production",
pages = "174-165",
number = "1",
volume = "12",
doi = "10.1007/s12649-020-00960-3"
}

Mihajlovski, K., Buntić, A., Milić, M., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production. in Waste and Biomass Valorization
Springer, Dordrecht., 12(1), 165-174.
https://doi.org/10.1007/s12649-020-00960-3

Mihajlovski K, Buntić A, Milić M, Rajilić-Stojanović M, Dimitrijević-Branković S. From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production. in Waste and Biomass Valorization. 2021;12(1):165-174.
doi:10.1007/s12649-020-00960-3 .

Mihajlovski, Katarina, Buntić, Aneta, Milić, Marija, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production" in Waste and Biomass Valorization, 12, no. 1 (2021):165-174,
https://doi.org/10.1007/s12649-020-00960-3 . .

TY  - JOUR
AU  - Rudić, Suzana
AU  - Dimitrijević-Branković, Suzana
AU  - Dimitrijević, Snežana
AU  - Milić, Marija
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4584
AB  - This study has developed an improved and fast microwave-assisted procedure for extraction of high yields of natural antioxidants from artichoke leaves dust. The material is derived as an abundant residue remained after processing for the industrial tea blends preparation, having no commercial value. A key factors for optimization of the total polyphenolic (TPC) and flavonoid content (TFC) and antioxidant activities have been determined by using single factor experiments along with a response surface methodology. The optimal conditions for maximizing the combination of TPC/TFC and antioxidant activity were obtained as follows: ethanol concentration 67.81%, extraction time 50 s, liquid/solid ratio 41.78 mL/g, microwave power 180 W, whereas the corresponding responses resulted in: TPC − 205.59 ± 7.30 mg GAE/g dw, TFC − 79.75 ± 1.12 mg QE/g dw, and FRAP −1008.14 ± 37.15 mmolFe2+/g dw. Only 14.7 µg/mL of the extract concentration is required to inhibit 50% of DPPH free radicals. A comparative study confirmed that the proposed procedure yielded higher TPC, TFC and antioxidant activities than available extraction methods. It highlights a several fold reduced extraction time, caring an eco-friendly principle for obtaining of antioxidants from industrial, medicinal crop residues, which would be further easily valorized in food and pharmaceutical industry.
PB  - Elsevier, Amsterdam
T2  - Separation and Purification Technology
T1  - Valorization of unexploited artichoke leaves dust for obtaining of extracts rich in natural antioxidants
SP  - 117714
VL  - 256
DO  - 10.1016/j.seppur.2020.117714
ER  - 

@article{
author = "Rudić, Suzana and Dimitrijević-Branković, Suzana and Dimitrijević, Snežana and Milić, Marija",
year = "2021",
abstract = "This study has developed an improved and fast microwave-assisted procedure for extraction of high yields of natural antioxidants from artichoke leaves dust. The material is derived as an abundant residue remained after processing for the industrial tea blends preparation, having no commercial value. A key factors for optimization of the total polyphenolic (TPC) and flavonoid content (TFC) and antioxidant activities have been determined by using single factor experiments along with a response surface methodology. The optimal conditions for maximizing the combination of TPC/TFC and antioxidant activity were obtained as follows: ethanol concentration 67.81%, extraction time 50 s, liquid/solid ratio 41.78 mL/g, microwave power 180 W, whereas the corresponding responses resulted in: TPC − 205.59 ± 7.30 mg GAE/g dw, TFC − 79.75 ± 1.12 mg QE/g dw, and FRAP −1008.14 ± 37.15 mmolFe2+/g dw. Only 14.7 µg/mL of the extract concentration is required to inhibit 50% of DPPH free radicals. A comparative study confirmed that the proposed procedure yielded higher TPC, TFC and antioxidant activities than available extraction methods. It highlights a several fold reduced extraction time, caring an eco-friendly principle for obtaining of antioxidants from industrial, medicinal crop residues, which would be further easily valorized in food and pharmaceutical industry.",
publisher = "Elsevier, Amsterdam",
journal = "Separation and Purification Technology",
title = "Valorization of unexploited artichoke leaves dust for obtaining of extracts rich in natural antioxidants",
pages = "117714",
volume = "256",
doi = "10.1016/j.seppur.2020.117714"
}

Rudić, S., Dimitrijević-Branković, S., Dimitrijević, S.,& Milić, M.. (2021). Valorization of unexploited artichoke leaves dust for obtaining of extracts rich in natural antioxidants. in Separation and Purification Technology
Elsevier, Amsterdam., 256, 117714.
https://doi.org/10.1016/j.seppur.2020.117714

Rudić S, Dimitrijević-Branković S, Dimitrijević S, Milić M. Valorization of unexploited artichoke leaves dust for obtaining of extracts rich in natural antioxidants. in Separation and Purification Technology. 2021;256:117714.
doi:10.1016/j.seppur.2020.117714 .

Rudić, Suzana, Dimitrijević-Branković, Suzana, Dimitrijević, Snežana, Milić, Marija, "Valorization of unexploited artichoke leaves dust for obtaining of extracts rich in natural antioxidants" in Separation and Purification Technology, 256 (2021):117714,
https://doi.org/10.1016/j.seppur.2020.117714 . .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Milić, Marija
AU  - Pecarski, Danijela
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4853
AB  - A recent trend in sustainable bioethanol production is the use of agricultural waste or food waste as an inexpensive and the most available feedstock. Bread waste is the major food waste that could be successfully used for the production of bioethanol. The aim of this study was to optimize ethanol production by the response surface methodology (RSM) using waste bread hydrolysate. Waste bread hydrolysate was obtained using crude hydrolytic enzymes that produce bacterial isolate Hymenobacter sp. CKS3. The influence of time of fermentation (24-72 h) and waste brewer's yeast inoculum (1-4 %) on ethanol production was studied. The optimal conditions, obtained by central composite design (CCD), were 48.6 h of fermentation and 2.85 % of inoculum. Under these conditions, a maximum of 2.06 % of ethanol concentration was reached. The obtained ethanol concentration was in good correlation, coefficient of 0.858, with yeast cell yield. The results obtained in this study imply that waste bread hydrolysate could be used as a biomass source for biofuel production with multiple benefits relating to environmental protection, reduction of production costs, and saving fossil fuels.
T2  - Journal of the Serbian Chemical Society
T1  - Statistical optimization of bioethanol production from waste bread hydrolysate
EP  - 662
IS  - 7-8
SP  - 651
VL  - 86
DO  - 10.2298/JSC210308032M
ER  - 

@article{
author = "Mihajlovski, Katarina and Milić, Marija and Pecarski, Danijela and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "A recent trend in sustainable bioethanol production is the use of agricultural waste or food waste as an inexpensive and the most available feedstock. Bread waste is the major food waste that could be successfully used for the production of bioethanol. The aim of this study was to optimize ethanol production by the response surface methodology (RSM) using waste bread hydrolysate. Waste bread hydrolysate was obtained using crude hydrolytic enzymes that produce bacterial isolate Hymenobacter sp. CKS3. The influence of time of fermentation (24-72 h) and waste brewer's yeast inoculum (1-4 %) on ethanol production was studied. The optimal conditions, obtained by central composite design (CCD), were 48.6 h of fermentation and 2.85 % of inoculum. Under these conditions, a maximum of 2.06 % of ethanol concentration was reached. The obtained ethanol concentration was in good correlation, coefficient of 0.858, with yeast cell yield. The results obtained in this study imply that waste bread hydrolysate could be used as a biomass source for biofuel production with multiple benefits relating to environmental protection, reduction of production costs, and saving fossil fuels.",
journal = "Journal of the Serbian Chemical Society",
title = "Statistical optimization of bioethanol production from waste bread hydrolysate",
pages = "662-651",
number = "7-8",
volume = "86",
doi = "10.2298/JSC210308032M"
}

Mihajlovski, K., Milić, M., Pecarski, D.,& Dimitrijević-Branković, S.. (2021). Statistical optimization of bioethanol production from waste bread hydrolysate. in Journal of the Serbian Chemical Society, 86(7-8), 651-662.
https://doi.org/10.2298/JSC210308032M

Mihajlovski K, Milić M, Pecarski D, Dimitrijević-Branković S. Statistical optimization of bioethanol production from waste bread hydrolysate. in Journal of the Serbian Chemical Society. 2021;86(7-8):651-662.
doi:10.2298/JSC210308032M .

Mihajlovski, Katarina, Milić, Marija, Pecarski, Danijela, Dimitrijević-Branković, Suzana, "Statistical optimization of bioethanol production from waste bread hydrolysate" in Journal of the Serbian Chemical Society, 86, no. 7-8 (2021):651-662,
https://doi.org/10.2298/JSC210308032M . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5939
AB  - The polymerization and crosslinking of various phenolic compounds induced by the laccase enzyme have been in recent years increasingly used in food industry, due to the emergence of products with improved properties, especially with increased antioxidant activity. In this regard, this study has explored the laccase-induced structural changes of two types of naturally occurring polyphenols, named gallic acid and epigallocatechin gallate, and their antioxidant activity. In the first part, the effect of the laccase of different origin - laccase of white rot fungi (Ganoderma spp.), previously grown on waste cereals and commercial laccase from Novozym® 51003, were investigated. Based on the results obtained after incubation (24 h, 50ºC), the sample containing a mixture of polyphenols, rather than individually, gave visible changes in the reading of the UV-Vis spectrum and increased antioxidant activity with small differences between commercial laccase and laccase of white rot fungi, which indicates that laccase obtained by growing fungi on waste cereals may be competitive for such a purpose. Moreover, the use of such an obtained laccase is more environmentally friendly and economically viable than use of commercial one. Further process optimization of polyphenols polymerization was performed within the method of multifactor statistical analysis (Design Expert), by using of commercial laccase, where by the effect of enzyme concentrations (0.1, 0.3 and 0.5 U/ml) and incubation time (4, 14 and 24 h) were monitored. The maximal antioxidant activity, measured by both DPPH (inhibition of 58.58% of radicals) and FRAP (176.57 mmol Fe2+/ml) was achieved by a sample containing a mixture of polyphenols and 0.3 U/ml laccase enzymes, after 14 h, at 50˚C. The results of this study revealed that careful optimization of process variables during polyphenols polymerization is extremely important for obtaining the product with desirable value added properties, which may be implemented in food and pharmaceutical industry.
C3  - Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021
T1  - The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme
SP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5939
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "The polymerization and crosslinking of various phenolic compounds induced by the laccase enzyme have been in recent years increasingly used in food industry, due to the emergence of products with improved properties, especially with increased antioxidant activity. In this regard, this study has explored the laccase-induced structural changes of two types of naturally occurring polyphenols, named gallic acid and epigallocatechin gallate, and their antioxidant activity. In the first part, the effect of the laccase of different origin - laccase of white rot fungi (Ganoderma spp.), previously grown on waste cereals and commercial laccase from Novozym® 51003, were investigated. Based on the results obtained after incubation (24 h, 50ºC), the sample containing a mixture of polyphenols, rather than individually, gave visible changes in the reading of the UV-Vis spectrum and increased antioxidant activity with small differences between commercial laccase and laccase of white rot fungi, which indicates that laccase obtained by growing fungi on waste cereals may be competitive for such a purpose. Moreover, the use of such an obtained laccase is more environmentally friendly and economically viable than use of commercial one. Further process optimization of polyphenols polymerization was performed within the method of multifactor statistical analysis (Design Expert), by using of commercial laccase, where by the effect of enzyme concentrations (0.1, 0.3 and 0.5 U/ml) and incubation time (4, 14 and 24 h) were monitored. The maximal antioxidant activity, measured by both DPPH (inhibition of 58.58% of radicals) and FRAP (176.57 mmol Fe2+/ml) was achieved by a sample containing a mixture of polyphenols and 0.3 U/ml laccase enzymes, after 14 h, at 50˚C. The results of this study revealed that careful optimization of process variables during polyphenols polymerization is extremely important for obtaining the product with desirable value added properties, which may be implemented in food and pharmaceutical industry.",
journal = "Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021",
title = "The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5939"
}

Ilić, N., Milić, M., Davidović, S., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2021). The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme. in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021, 158.
https://hdl.handle.net/21.15107/rcub_technorep_5939

Ilić N, Milić M, Davidović S, Mihajlovski K, Dimitrijević-Branković S. The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme. in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021. 2021;:158.
https://hdl.handle.net/21.15107/rcub_technorep_5939 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme" in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021 (2021):158,
https://hdl.handle.net/21.15107/rcub_technorep_5939 .

TY  - JOUR
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Đukić-Vuković, Aleksandra
AU  - Ilic, Mila, V
AU  - Simović, Milica
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4791
AB  - Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391 +/- 0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.
T2  - Hemijska industrija
T1  - Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology
EP  - 146
IS  - 3
SP  - 135
VL  - 75
DO  - 10.2298/HEMIND200710015M
ER  - 

@article{
author = "Miljković, Miona and Davidović, Slađana and Đukić-Vuković, Aleksandra and Ilic, Mila, V and Simović, Milica and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391 +/- 0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.",
journal = "Hemijska industrija",
title = "Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology",
pages = "146-135",
number = "3",
volume = "75",
doi = "10.2298/HEMIND200710015M"
}

Miljković, M., Davidović, S., Đukić-Vuković, A., Ilic, M. V., Simović, M., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology. in Hemijska industrija, 75(3), 135-146.
https://doi.org/10.2298/HEMIND200710015M

Miljković M, Davidović S, Đukić-Vuković A, Ilic MV, Simović M, Rajilić-Stojanović M, Dimitrijević-Branković S. Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology. in Hemijska industrija. 2021;75(3):135-146.
doi:10.2298/HEMIND200710015M .

Miljković, Miona, Davidović, Slađana, Đukić-Vuković, Aleksandra, Ilic, Mila, V, Simović, Milica, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology" in Hemijska industrija, 75, no. 3 (2021):135-146,
https://doi.org/10.2298/HEMIND200710015M . .