TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Avdović, Edina
AU  - Plećić, Ana
AU  - Čutović, Natalija
AU  - Bugarski, Branko
AU  - Marković, Zoran
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7440
AB  - Coumarin  derivates  possess  several  biological  effects,  including  anti-inflammatory,  antioxidant, anticoagulant, antitumor, insecticidal, anthelminthic, hypnotic, antifungal, and HIV protease inhibition properties [1,2]. Due to their solubility in organic solvents and insolubility in water and body fluids, their bioavailability is significantlylow.  Thus,  they  can  be  encapsulated  intoliposomal  particles  with  the  aim  of  overcoming  the  mentioned  disadvan-tage[3]. Hence, in the present study, coumarin derivate was encapsulated in phospholipid liposomes and their stability was monitored for 60 days in terms of vesicle size, polydispersity index (PDI), zeta potential, and mobility.
PB  - Savez hemijskih inženjera Srbije
C3  - Hemijska industrija
T1  - Stability of liposomal particles with encapsulated coumarin derivate
IS  - 1S
SP  - 66
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7440
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Avdović, Edina and Plećić, Ana and Čutović, Natalija and Bugarski, Branko and Marković, Zoran",
year = "2024",
abstract = "Coumarin  derivates  possess  several  biological  effects,  including  anti-inflammatory,  antioxidant, anticoagulant, antitumor, insecticidal, anthelminthic, hypnotic, antifungal, and HIV protease inhibition properties [1,2]. Due to their solubility in organic solvents and insolubility in water and body fluids, their bioavailability is significantlylow.  Thus,  they  can  be  encapsulated  intoliposomal  particles  with  the  aim  of  overcoming  the  mentioned  disadvan-tage[3]. Hence, in the present study, coumarin derivate was encapsulated in phospholipid liposomes and their stability was monitored for 60 days in terms of vesicle size, polydispersity index (PDI), zeta potential, and mobility.",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija",
title = "Stability of liposomal particles with encapsulated coumarin derivate",
number = "1S",
pages = "66",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7440"
}

Jovanović, A. A., Avdović, E., Plećić, A., Čutović, N., Bugarski, B.,& Marković, Z.. (2024). Stability of liposomal particles with encapsulated coumarin derivate. in Hemijska industrija
Savez hemijskih inženjera Srbije., 78(1S), 66.
https://hdl.handle.net/21.15107/rcub_technorep_7440

Jovanović AA, Avdović E, Plećić A, Čutović N, Bugarski B, Marković Z. Stability of liposomal particles with encapsulated coumarin derivate. in Hemijska industrija. 2024;78(1S):66.
https://hdl.handle.net/21.15107/rcub_technorep_7440 .

Jovanović, Aleksandra A., Avdović, Edina, Plećić, Ana, Čutović, Natalija, Bugarski, Branko, Marković, Zoran, "Stability of liposomal particles with encapsulated coumarin derivate" in Hemijska industrija, 78, no. 1S (2024):66,
https://hdl.handle.net/21.15107/rcub_technorep_7440 .

TY  - CONF
AU  - Elferjane, Muna Rajab
AU  - Milošević, Milena
AU  - Ćirić, Vojislav
AU  - Batinić, Petar
AU  - Bugarski, Branko
AU  - Marinković, Aleksandar
AU  - Jovanović, Aleksandra A.
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7437
AB  - Vaccinium myrtillus L. leaves contain bioactive components, such as polyphenols, stilbenes, iridoid glycosides, fatty acids, and fibers. However, polyphenols possess low solubility, stability, and bioavailability, thus the encapsulation of the mentioned active principles in different carriers is necessary. Liposomes are widely used as a carrier for the encapsulation, preservation, and controlled release of polyphenols in various products. Therefore, the aims of the presented research are the development and characterization of V. myrillus leaf extract-loaded liposomes via the determination of encapsulation efficiency, particle size, polydispersity index (PDI), zeta potential, and mobility.
PB  - Savez hemijskih inženjera Srbije
C3  - Hemijska industrija
T1  - Characterization of Vaccinium myrtillus leaf extract-loaded liposomes
IS  - 1S
SP  - 65
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7437
ER  - 

@conference{
author = "Elferjane, Muna Rajab and Milošević, Milena and Ćirić, Vojislav and Batinić, Petar and Bugarski, Branko and Marinković, Aleksandar and Jovanović, Aleksandra A.",
year = "2024",
abstract = "Vaccinium myrtillus L. leaves contain bioactive components, such as polyphenols, stilbenes, iridoid glycosides, fatty acids, and fibers. However, polyphenols possess low solubility, stability, and bioavailability, thus the encapsulation of the mentioned active principles in different carriers is necessary. Liposomes are widely used as a carrier for the encapsulation, preservation, and controlled release of polyphenols in various products. Therefore, the aims of the presented research are the development and characterization of V. myrillus leaf extract-loaded liposomes via the determination of encapsulation efficiency, particle size, polydispersity index (PDI), zeta potential, and mobility.",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija",
title = "Characterization of Vaccinium myrtillus leaf extract-loaded liposomes",
number = "1S",
pages = "65",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7437"
}

Elferjane, M. R., Milošević, M., Ćirić, V., Batinić, P., Bugarski, B., Marinković, A.,& Jovanović, A. A.. (2024). Characterization of Vaccinium myrtillus leaf extract-loaded liposomes. in Hemijska industrija
Savez hemijskih inženjera Srbije., 78(1S), 65.
https://hdl.handle.net/21.15107/rcub_technorep_7437

Elferjane MR, Milošević M, Ćirić V, Batinić P, Bugarski B, Marinković A, Jovanović AA. Characterization of Vaccinium myrtillus leaf extract-loaded liposomes. in Hemijska industrija. 2024;78(1S):65.
https://hdl.handle.net/21.15107/rcub_technorep_7437 .

Elferjane, Muna Rajab, Milošević, Milena, Ćirić, Vojislav, Batinić, Petar, Bugarski, Branko, Marinković, Aleksandar, Jovanović, Aleksandra A., "Characterization of Vaccinium myrtillus leaf extract-loaded liposomes" in Hemijska industrija, 78, no. 1S (2024):65,
https://hdl.handle.net/21.15107/rcub_technorep_7437 .

TY  - JOUR
AU  - Bošković, Stefan A.
AU  - Bugarski, Branko
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7288
AB  - In this work, we present a two-phase electro-magneto-fluid dynamics (EMFD) model that merges electromagnetics and fluid dynamics. The model is suitable for use in computational fluid dynamics (CFD) software and was incorporated into OpenFOAM® after deriving appropriate equations that bypassed certain limitations of the software. Currently, there is a lack of even single-phase EMFD models that can be incorporated in CFD software, while simpler models from electrohydrodynamics (EHD) and magnetohydrodynamics (MHD) are being implemented, though they have certain approximations that can limit their applicability. We conclude that the derived EMFD model is applicable and show its quality by implementing it and analyzing the results. We use cases with a droplet and the electrospinning process for verification. The drop deformations obtained were closer to analytical predictions than in the literature for two EHD models, but some oscillations were observed. We compared one simulation to the prediction of an analytical equation from MHD, and good agreement was shown. Finally, we simulate the electrospinning process, and the results were very close to the analytical predictions. We conclude that the implementation can be used for both EHD and MHD cases.
PB  - AIP Publishing
T2  - Physics of Fluids
T1  - Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation
IS  - 2
SP  - 022019
VL  - 36
DO  - 10.1063/5.0190651
ER  - 

@article{
author = "Bošković, Stefan A. and Bugarski, Branko",
year = "2024",
abstract = "In this work, we present a two-phase electro-magneto-fluid dynamics (EMFD) model that merges electromagnetics and fluid dynamics. The model is suitable for use in computational fluid dynamics (CFD) software and was incorporated into OpenFOAM® after deriving appropriate equations that bypassed certain limitations of the software. Currently, there is a lack of even single-phase EMFD models that can be incorporated in CFD software, while simpler models from electrohydrodynamics (EHD) and magnetohydrodynamics (MHD) are being implemented, though they have certain approximations that can limit their applicability. We conclude that the derived EMFD model is applicable and show its quality by implementing it and analyzing the results. We use cases with a droplet and the electrospinning process for verification. The drop deformations obtained were closer to analytical predictions than in the literature for two EHD models, but some oscillations were observed. We compared one simulation to the prediction of an analytical equation from MHD, and good agreement was shown. Finally, we simulate the electrospinning process, and the results were very close to the analytical predictions. We conclude that the implementation can be used for both EHD and MHD cases.",
publisher = "AIP Publishing",
journal = "Physics of Fluids",
title = "Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation",
number = "2",
pages = "022019",
volume = "36",
doi = "10.1063/5.0190651"
}

Bošković, S. A.,& Bugarski, B.. (2024). Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation. in Physics of Fluids
AIP Publishing., 36(2), 022019.
https://doi.org/10.1063/5.0190651

Bošković SA, Bugarski B. Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation. in Physics of Fluids. 2024;36(2):022019.
doi:10.1063/5.0190651 .

Bošković, Stefan A., Bugarski, Branko, "Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation" in Physics of Fluids, 36, no. 2 (2024):022019,
https://doi.org/10.1063/5.0190651 . .

TY  - JOUR
AU  - Bošković, Stefan A.
AU  - Bugarski, Branko
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7348
AB  - In this work, we present a two-phase electro-magneto-fluid dynamics (EMFD) model that merges electromagnetics and fluid dynamics. The model is suitable for use in computational fluid dynamics (CFD) software and was incorporated into OpenFOAM® after deriving appropriate equations that bypassed certain limitations of the software. Currently, there is lack of even single-phase EMFD models that can be incorporated into CFD software; however, simpler models from electrohydrodynamics (EHD) and magnetohydrodynamics (MHD) are being implemented although they have certain approximations that can limit their applicability. We conclude that the derived EMFD model is applicable, and show its quality by implementing it and analyzing the results. We use cases with a droplet and the electrospinning process for verification. The drop deformations obtained were closer to analytical predictions than in the literature for two EHD models, but some oscillations were observed. We compared one simulation to the prediction of an analytical equation from MHD, and good agreement was shown. Finally, we simulate the electrospinning process, and the results were very close to the analytical predictions. We conclude that the implementation can be used for both EHD and MHD cases.
PB  - American Institute of Physics Inc.
T2  - Physics of Fluids
T1  - Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation
IS  - 2
SP  - 022019
VL  - 36
DO  - 10.1063/5.0190651
ER  - 

@article{
author = "Bošković, Stefan A. and Bugarski, Branko",
year = "2024",
abstract = "In this work, we present a two-phase electro-magneto-fluid dynamics (EMFD) model that merges electromagnetics and fluid dynamics. The model is suitable for use in computational fluid dynamics (CFD) software and was incorporated into OpenFOAM® after deriving appropriate equations that bypassed certain limitations of the software. Currently, there is lack of even single-phase EMFD models that can be incorporated into CFD software; however, simpler models from electrohydrodynamics (EHD) and magnetohydrodynamics (MHD) are being implemented although they have certain approximations that can limit their applicability. We conclude that the derived EMFD model is applicable, and show its quality by implementing it and analyzing the results. We use cases with a droplet and the electrospinning process for verification. The drop deformations obtained were closer to analytical predictions than in the literature for two EHD models, but some oscillations were observed. We compared one simulation to the prediction of an analytical equation from MHD, and good agreement was shown. Finally, we simulate the electrospinning process, and the results were very close to the analytical predictions. We conclude that the implementation can be used for both EHD and MHD cases.",
publisher = "American Institute of Physics Inc.",
journal = "Physics of Fluids",
title = "Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation",
number = "2",
pages = "022019",
volume = "36",
doi = "10.1063/5.0190651"
}

Bošković, S. A.,& Bugarski, B.. (2024). Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation. in Physics of Fluids
American Institute of Physics Inc.., 36(2), 022019.
https://doi.org/10.1063/5.0190651

Bošković SA, Bugarski B. Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation. in Physics of Fluids. 2024;36(2):022019.
doi:10.1063/5.0190651 .

Bošković, Stefan A., Bugarski, Branko, "Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation" in Physics of Fluids, 36, no. 2 (2024):022019,
https://doi.org/10.1063/5.0190651 . .

TY  - JOUR
AU  - Đukić, Tamara
AU  - Drvenica, Ivana
AU  - Kovačić, Marijana
AU  - Milanović, Slađan
AU  - Majerič, Dragana
AU  - Šefik-Bukilica, Mirjana
AU  - Miletić, Maja
AU  - Bugarski, Branko
AU  - Ilić, Vesna
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7411
AB  - The function of immune complexes in rheumatoid arthritis (RA) is related to their composition and size. Using dynamic light scattering (DLS), we investigated the link between the RA circulating immune complex (CIC) particles’ size and the CIC immunoglobulin level. In this study, 30 RA patients and 30 healthy individuals were included. IgA, IgG, and IgM were found in all analyzed CICs, but more IgA and IgG were found in RA than in control CICs. In both control and RA CICs, DLS detected 50 particles that differed in size and clustered around two size groups: with a 7.5–164 nm radius and with a 342–1718 nm radius. An increased level of IgA in RA CICs, compared to control ones, was associated with more than 50% of CIC particles. In RA, compared to the control, a higher number of CICs with 28.2 nm, 531 nm, 712 nm, and 1718 nm particles and a lower number of CICs with 78.8 nm particles were detected. This particle distribution pattern did not reflect the changes in the CIC immunoglobulin level. Thus, RA elevated CIC IgA was linked with all these particles (except the 1718 nm particle), the IgM increase was linked with 43.8 nm and 712 nm particles, and the IgG increase was linked with the 712 nm particle only. This study provides the very first data on the association between CIC particles’ size, CIC immunoglobulin level, and RA. It opens the possibility that the size of CICs determined by DLS can be used as a criterion in RA diagnosis or monitoring after a large-scale study confirmation.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients
IS  - 6
SP  - 3138
VL  - 25
DO  - 10.3390/ijms25063138
ER  - 

@article{
author = "Đukić, Tamara and Drvenica, Ivana and Kovačić, Marijana and Milanović, Slađan and Majerič, Dragana and Šefik-Bukilica, Mirjana and Miletić, Maja and Bugarski, Branko and Ilić, Vesna",
year = "2024",
abstract = "The function of immune complexes in rheumatoid arthritis (RA) is related to their composition and size. Using dynamic light scattering (DLS), we investigated the link between the RA circulating immune complex (CIC) particles’ size and the CIC immunoglobulin level. In this study, 30 RA patients and 30 healthy individuals were included. IgA, IgG, and IgM were found in all analyzed CICs, but more IgA and IgG were found in RA than in control CICs. In both control and RA CICs, DLS detected 50 particles that differed in size and clustered around two size groups: with a 7.5–164 nm radius and with a 342–1718 nm radius. An increased level of IgA in RA CICs, compared to control ones, was associated with more than 50% of CIC particles. In RA, compared to the control, a higher number of CICs with 28.2 nm, 531 nm, 712 nm, and 1718 nm particles and a lower number of CICs with 78.8 nm particles were detected. This particle distribution pattern did not reflect the changes in the CIC immunoglobulin level. Thus, RA elevated CIC IgA was linked with all these particles (except the 1718 nm particle), the IgM increase was linked with 43.8 nm and 712 nm particles, and the IgG increase was linked with the 712 nm particle only. This study provides the very first data on the association between CIC particles’ size, CIC immunoglobulin level, and RA. It opens the possibility that the size of CICs determined by DLS can be used as a criterion in RA diagnosis or monitoring after a large-scale study confirmation.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients",
number = "6",
pages = "3138",
volume = "25",
doi = "10.3390/ijms25063138"
}

Đukić, T., Drvenica, I., Kovačić, M., Milanović, S., Majerič, D., Šefik-Bukilica, M., Miletić, M., Bugarski, B.,& Ilić, V.. (2024). Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients. in International Journal of Molecular Sciences
MDPI., 25(6), 3138.
https://doi.org/10.3390/ijms25063138

Đukić T, Drvenica I, Kovačić M, Milanović S, Majerič D, Šefik-Bukilica M, Miletić M, Bugarski B, Ilić V. Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients. in International Journal of Molecular Sciences. 2024;25(6):3138.
doi:10.3390/ijms25063138 .

Đukić, Tamara, Drvenica, Ivana, Kovačić, Marijana, Milanović, Slađan, Majerič, Dragana, Šefik-Bukilica, Mirjana, Miletić, Maja, Bugarski, Branko, Ilić, Vesna, "Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients" in International Journal of Molecular Sciences, 25, no. 6 (2024):3138,
https://doi.org/10.3390/ijms25063138 . .

TY  - JOUR
AU  - Batinić, Petar M.
AU  - Đorđević, Verica B.
AU  - Obradović, Nataša S.
AU  - Krstić, Aleksandar D.
AU  - Stevanović, Sanja I.
AU  - Balanč, Bojana D.
AU  - Marković, Smilja B.
AU  - Pjanović, Rada V.
AU  - Mijin, Dušan Ž.
AU  - Bugarski, Branko M.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6358
AB  - Liposome-encapsulated folic acid is incorporated into the films made from sodium carboxymethyl cellulose (CMC) (2 mas%) and a mixture of CMC and solagum (9:1 w/w) using the film-forming cast solution method. Histidine is used to increase solubility for folic acid in liposomes (1–5 mg mL−1), and propylene glycol is used as a film plasticizer (2.6 mas%). The obtained films (50–60 µm tick) containing 3.12–20.19 mg of folic acid per gram of film are envisaged to be used as patches for transdermal delivery of folic acid. Therefore, some physical, mechanical, release and structural attributes of the films are scrutinized. Folic acid gives yellow color to the films and contributes to stronger chemical bonds which result in improved strength of the film. Liposomes prolong the release of folic acid from films to 24 h without adverse effects on mechanical properties of the films, but degrade homogeneity of the films, which can be ascribed to its agglomeration within the film matrix as revealed by atomic force microscopy. According to the release at pH 5.5, the film formulation based on a blend of CMC and solagum containing 3 mg mL−1 liposome-encapsulated folic acid is recommended. Practical Application: Folic acid is effective in reducing oxidative stress levels in the skin and neutralizing the harmful free radicals and is also essential for various metabolic reactions in the body. However, the limited solubility of folic acid linked with its poor absorption in an organism, low storage stability, short half-life upon oral consumption, specific food preferences of some people, extensive liver metabolism, and pregnancy-induced vomiting point to a large potential in transdermal usage of folic acid. This has motivated us to design new multicomponent polymer-lipid systems as an alternative solution to overcome some of these drawbacks. The results obtained for these multicomponent films pointed to their potential for prolonged release of folic acid to 24 h, which can also be useful for scientists interested in encapsulating similar poorly soluble compounds in CMC patches. The finding can be also valuable information for pharmaceutical manufacturers and scientists worldwide.
PB  - John Wiley and Sons Inc.
T2  - European Journal of Lipid Science and Technology
T1  - Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid
SP  - 2200169
DO  - 10.1002/ejlt.202200169
ER  - 

@article{
author = "Batinić, Petar M. and Đorđević, Verica B. and Obradović, Nataša S. and Krstić, Aleksandar D. and Stevanović, Sanja I. and Balanč, Bojana D. and Marković, Smilja B. and Pjanović, Rada V. and Mijin, Dušan Ž. and Bugarski, Branko M.",
year = "2023",
abstract = "Liposome-encapsulated folic acid is incorporated into the films made from sodium carboxymethyl cellulose (CMC) (2 mas%) and a mixture of CMC and solagum (9:1 w/w) using the film-forming cast solution method. Histidine is used to increase solubility for folic acid in liposomes (1–5 mg mL−1), and propylene glycol is used as a film plasticizer (2.6 mas%). The obtained films (50–60 µm tick) containing 3.12–20.19 mg of folic acid per gram of film are envisaged to be used as patches for transdermal delivery of folic acid. Therefore, some physical, mechanical, release and structural attributes of the films are scrutinized. Folic acid gives yellow color to the films and contributes to stronger chemical bonds which result in improved strength of the film. Liposomes prolong the release of folic acid from films to 24 h without adverse effects on mechanical properties of the films, but degrade homogeneity of the films, which can be ascribed to its agglomeration within the film matrix as revealed by atomic force microscopy. According to the release at pH 5.5, the film formulation based on a blend of CMC and solagum containing 3 mg mL−1 liposome-encapsulated folic acid is recommended. Practical Application: Folic acid is effective in reducing oxidative stress levels in the skin and neutralizing the harmful free radicals and is also essential for various metabolic reactions in the body. However, the limited solubility of folic acid linked with its poor absorption in an organism, low storage stability, short half-life upon oral consumption, specific food preferences of some people, extensive liver metabolism, and pregnancy-induced vomiting point to a large potential in transdermal usage of folic acid. This has motivated us to design new multicomponent polymer-lipid systems as an alternative solution to overcome some of these drawbacks. The results obtained for these multicomponent films pointed to their potential for prolonged release of folic acid to 24 h, which can also be useful for scientists interested in encapsulating similar poorly soluble compounds in CMC patches. The finding can be also valuable information for pharmaceutical manufacturers and scientists worldwide.",
publisher = "John Wiley and Sons Inc.",
journal = "European Journal of Lipid Science and Technology",
title = "Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid",
pages = "2200169",
doi = "10.1002/ejlt.202200169"
}

Batinić, P. M., Đorđević, V. B., Obradović, N. S., Krstić, A. D., Stevanović, S. I., Balanč, B. D., Marković, S. B., Pjanović, R. V., Mijin, D. Ž.,& Bugarski, B. M.. (2023). Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid. in European Journal of Lipid Science and Technology
John Wiley and Sons Inc.., 2200169.
https://doi.org/10.1002/ejlt.202200169

Batinić PM, Đorđević VB, Obradović NS, Krstić AD, Stevanović SI, Balanč BD, Marković SB, Pjanović RV, Mijin DŽ, Bugarski BM. Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid. in European Journal of Lipid Science and Technology. 2023;:2200169.
doi:10.1002/ejlt.202200169 .

Batinić, Petar M., Đorđević, Verica B., Obradović, Nataša S., Krstić, Aleksandar D., Stevanović, Sanja I., Balanč, Bojana D., Marković, Smilja B., Pjanović, Rada V., Mijin, Dušan Ž., Bugarski, Branko M., "Polymer-Lipid Matrices based on Carboxymethyl Cellulose/Solagum and Liposomes for Controlled Release of Folic Acid" in European Journal of Lipid Science and Technology (2023):2200169,
https://doi.org/10.1002/ejlt.202200169 . .

TY  - CONF
AU  - Jovanović, Aleksandra
AU  - Volić, Mina
AU  - Obradović, Nataša
AU  - Bugarski, Branko
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6452
AB  - Polyphenol recovery and physico-chemical properties of the extracts depend on the type of
solvents and their pH values. Additionally, due to the presence of different biologically active
compounds in plant waste, plant extracts obtained from industrial by-processing can find
potential applications in various products. In the present study, Serpylli herba waste extracts
were prepared using maceration (60 min), a solid-to-solvent ratio of 1:30, the particle size of
plant waste 0.3 mm, and two types of the extraction medium: 50% ethanol (pH 6) and 50%
ethanol with glacial acetic acid (pH 2.5). The lyophilization process was chosen as the next
step (-75°C, 0.011 mbar, for 24 h). Comparison of liquid and lyophilized extracts prepared at
different pH values was done via analyzing total polyphenol content (TPC, Folin-Ciocalteu
method), total flavonoid content (TFC, colorimetric assay), antioxidant capacity (ABTS and
DPPH assays), zeta potential, and conductivity (photon correlation spectroscopy). TPC of
liquid extracts prepared at pH 2.5 and pH 6 amounted to 1.38 and 1.23 mg GAE/mL,
respectively, while lyophilized parallels had 271.7 and 188.8 mg GAE/g, respectively. The
same trend is noticed in the case of TFC: 0.368 and 0.334 mg CE/mL for liquid extracts
obtained at pH 2.5 and pH 6, respectively, and 102.5 and 99.7 mg CE/mL for lyophilized
parallels. ABTS radical scavenging activity of the liquid extracts at pH 2.5 and pH 6 was
0.767 and 0.750 mmol TE/L, respectively and for the lyophilized parallels was 0.136 and
0.111 mmol TE/g. IC50 (concentration for neutralization of 50% of DPPH free radicals) was
1.12 and 1.75 mg/mL for the liquid extracts prepared at pH 2.5 and pH 6, respectively and
0.331 and 0.391 mg/mL for the lyophilized parallels. The zeta potential of the liquid extracts
at pH 2.5 and pH 6 was 1.74 and -2.56 mV, respectively, whereas the zeta potential of
lyophilized parallels was -3.55 and -18.7 mV. Conductivity of the liquid extracts was 0.864(pH 2.5) and 0.423 mS/cm (pH 6), whereas for the lyophilized extracts it was 0.199 (pH 2.5)
and 0.452 mS/cm (pH 6). The presented results provide the information on physico-chemical
properties of Serpylli herba waste liquid and lyophilized extracts that can add value and
improve the quality of the existing food, functional food, pharmaceutical and cosmetic
products, as well as for drinking water and wastewater treatment.
PB  - Novi Sad : Faculty of Technology
C3  - Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad
T1  - COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES
EP  - 175
SP  - 166
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6452
ER  - 

@conference{
author = "Jovanović, Aleksandra and Volić, Mina and Obradović, Nataša and Bugarski, Branko",
year = "2023",
abstract = "Polyphenol recovery and physico-chemical properties of the extracts depend on the type of
solvents and their pH values. Additionally, due to the presence of different biologically active
compounds in plant waste, plant extracts obtained from industrial by-processing can find
potential applications in various products. In the present study, Serpylli herba waste extracts
were prepared using maceration (60 min), a solid-to-solvent ratio of 1:30, the particle size of
plant waste 0.3 mm, and two types of the extraction medium: 50% ethanol (pH 6) and 50%
ethanol with glacial acetic acid (pH 2.5). The lyophilization process was chosen as the next
step (-75°C, 0.011 mbar, for 24 h). Comparison of liquid and lyophilized extracts prepared at
different pH values was done via analyzing total polyphenol content (TPC, Folin-Ciocalteu
method), total flavonoid content (TFC, colorimetric assay), antioxidant capacity (ABTS and
DPPH assays), zeta potential, and conductivity (photon correlation spectroscopy). TPC of
liquid extracts prepared at pH 2.5 and pH 6 amounted to 1.38 and 1.23 mg GAE/mL,
respectively, while lyophilized parallels had 271.7 and 188.8 mg GAE/g, respectively. The
same trend is noticed in the case of TFC: 0.368 and 0.334 mg CE/mL for liquid extracts
obtained at pH 2.5 and pH 6, respectively, and 102.5 and 99.7 mg CE/mL for lyophilized
parallels. ABTS radical scavenging activity of the liquid extracts at pH 2.5 and pH 6 was
0.767 and 0.750 mmol TE/L, respectively and for the lyophilized parallels was 0.136 and
0.111 mmol TE/g. IC50 (concentration for neutralization of 50% of DPPH free radicals) was
1.12 and 1.75 mg/mL for the liquid extracts prepared at pH 2.5 and pH 6, respectively and
0.331 and 0.391 mg/mL for the lyophilized parallels. The zeta potential of the liquid extracts
at pH 2.5 and pH 6 was 1.74 and -2.56 mV, respectively, whereas the zeta potential of
lyophilized parallels was -3.55 and -18.7 mV. Conductivity of the liquid extracts was 0.864(pH 2.5) and 0.423 mS/cm (pH 6), whereas for the lyophilized extracts it was 0.199 (pH 2.5)
and 0.452 mS/cm (pH 6). The presented results provide the information on physico-chemical
properties of Serpylli herba waste liquid and lyophilized extracts that can add value and
improve the quality of the existing food, functional food, pharmaceutical and cosmetic
products, as well as for drinking water and wastewater treatment.",
publisher = "Novi Sad : Faculty of Technology",
journal = "Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad",
title = "COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES",
pages = "175-166",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6452"
}

Jovanović, A., Volić, M., Obradović, N.,& Bugarski, B.. (2023). COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES. in Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad
Novi Sad : Faculty of Technology., 166-175.
https://hdl.handle.net/21.15107/rcub_technorep_6452

Jovanović A, Volić M, Obradović N, Bugarski B. COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES. in Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad. 2023;:166-175.
https://hdl.handle.net/21.15107/rcub_technorep_6452 .

Jovanović, Aleksandra, Volić, Mina, Obradović, Nataša, Bugarski, Branko, "COMPARISON OF LIQUID AND LYOPHILIZED SERPYLLI HERBA WASTE EXTRACTS PREPARED AT DIFFERENT pH VALUES" in Proceedings of the 2nd International Conference on Advanced Production and Processing ICAPP 2022, Novi Sad (2023):166-175,
https://hdl.handle.net/21.15107/rcub_technorep_6452 .

TY  - JOUR
AU  - Đukić, Tamara
AU  - Drvenica, Ivana
AU  - Kovačić, Marijana
AU  - Minić, Rajna
AU  - Vučetić, Dušan
AU  - Majerič, Dragana
AU  - Šefik-Bukilica, Mirjana
AU  - Savić, Olivera
AU  - Bugarski, Branko
AU  - Ilić, Vesna
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6470
AB  - The size of circulating immune complexes (CICs) in rheumatoid arthritis (RA) could be an emerging criterion in disease diagnosis. This study analyzed size and electrokinetic potential of CICs from RA patients, healthy young adults, and RA patients age-matched controls aiming to establish their unique CIC features. Pooled CIC of 30 RA patients, 30 young adults, and 30 RA group's age-matched controls (middle-aged and oldеr healthy adults), and in vitro IgG aggregates from pooled sera of 300 healthy volunteers were tested using dynamic light scattering (DLS). Size distribution of CIC in healthy young adults exhibited high polydispersity. RA CIC patients and their age-matched control showed distinctly narrower size distributions compared with young adults. In these groups, particles clustered around two well-defined peaks. Particles of peak 1 were 36.1 ± 6.8 nm in RA age-matched control, and 30.8 ± 4.2 nm in RA patients. Particles of peak 2 of the RA age-matched control's CIC was 251.7 ± 41.2 nm, while RA CIC contained larger particles (359.9 ± 50.5 nm). The lower zeta potential of RA CIC, compared to control, indicated a disease-related decrease in colloidal stability. DLS identified RA-specific, but also age-specific distribution of CIC size and opened possibility of becoming a method for CIC size analysis in IC-mediated diseases.
PB  - Academic Press Inc.
T2  - Analytical Biochemistry
T1  - Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients
SP  - 115194
VL  - 674
DO  - 10.1016/j.ab.2023.115194
ER  - 

@article{
author = "Đukić, Tamara and Drvenica, Ivana and Kovačić, Marijana and Minić, Rajna and Vučetić, Dušan and Majerič, Dragana and Šefik-Bukilica, Mirjana and Savić, Olivera and Bugarski, Branko and Ilić, Vesna",
year = "2023",
abstract = "The size of circulating immune complexes (CICs) in rheumatoid arthritis (RA) could be an emerging criterion in disease diagnosis. This study analyzed size and electrokinetic potential of CICs from RA patients, healthy young adults, and RA patients age-matched controls aiming to establish their unique CIC features. Pooled CIC of 30 RA patients, 30 young adults, and 30 RA group's age-matched controls (middle-aged and oldеr healthy adults), and in vitro IgG aggregates from pooled sera of 300 healthy volunteers were tested using dynamic light scattering (DLS). Size distribution of CIC in healthy young adults exhibited high polydispersity. RA CIC patients and their age-matched control showed distinctly narrower size distributions compared with young adults. In these groups, particles clustered around two well-defined peaks. Particles of peak 1 were 36.1 ± 6.8 nm in RA age-matched control, and 30.8 ± 4.2 nm in RA patients. Particles of peak 2 of the RA age-matched control's CIC was 251.7 ± 41.2 nm, while RA CIC contained larger particles (359.9 ± 50.5 nm). The lower zeta potential of RA CIC, compared to control, indicated a disease-related decrease in colloidal stability. DLS identified RA-specific, but also age-specific distribution of CIC size and opened possibility of becoming a method for CIC size analysis in IC-mediated diseases.",
publisher = "Academic Press Inc.",
journal = "Analytical Biochemistry",
title = "Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients",
pages = "115194",
volume = "674",
doi = "10.1016/j.ab.2023.115194"
}

Đukić, T., Drvenica, I., Kovačić, M., Minić, R., Vučetić, D., Majerič, D., Šefik-Bukilica, M., Savić, O., Bugarski, B.,& Ilić, V.. (2023). Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients. in Analytical Biochemistry
Academic Press Inc.., 674, 115194.
https://doi.org/10.1016/j.ab.2023.115194

Đukić T, Drvenica I, Kovačić M, Minić R, Vučetić D, Majerič D, Šefik-Bukilica M, Savić O, Bugarski B, Ilić V. Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients. in Analytical Biochemistry. 2023;674:115194.
doi:10.1016/j.ab.2023.115194 .

Đukić, Tamara, Drvenica, Ivana, Kovačić, Marijana, Minić, Rajna, Vučetić, Dušan, Majerič, Dragana, Šefik-Bukilica, Mirjana, Savić, Olivera, Bugarski, Branko, Ilić, Vesna, "Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients" in Analytical Biochemistry, 674 (2023):115194,
https://doi.org/10.1016/j.ab.2023.115194 . .

Knežević Jugović, Z., Đorđević, V., Antov, M., Šekuljica, N., Mijalković, J., Jakovetić Tanasković, S., Balanč, B., Petrović, P., Salević-Jelić, A., Rakić, V., Nedović, V.,& Bugarski, B.. (2023). Колачићи на бази протеина нове генерације – протеина из лишћа уљарица. in ИГРАЈ за човечанство! : наука за свe : каталог изложбене поставке 65. Међународни сајам технике и техничких достигнућа
Београд : Центар за промоцију науке., 37.
https://hdl.handle.net/21.15107/rcub_technorep_7078

Knežević Jugović Z, Đorđević V, Antov M, Šekuljica N, Mijalković J, Jakovetić Tanasković S, Balanč B, Petrović P, Salević-Jelić A, Rakić V, Nedović V, Bugarski B. Колачићи на бази протеина нове генерације – протеина из лишћа уљарица. in ИГРАЈ за човечанство! : наука за свe : каталог изложбене поставке 65. Међународни сајам технике и техничких достигнућа. 2023;:37.
https://hdl.handle.net/21.15107/rcub_technorep_7078 .

Knežević Jugović, Zorica, Đorđević, Verica, Antov, Mirjana, Šekuljica, Nataša, Mijalković, Jelena, Jakovetić Tanasković, Sonja, Balanč, Bojana, Petrović, Predrag, Salević-Jelić, Ana, Rakić, Vesna, Nedović, Viktor, Bugarski, Branko, "Колачићи на бази протеина нове генерације – протеина из лишћа уљарица" in ИГРАЈ за човечанство! : наука за свe : каталог изложбене поставке 65. Међународни сајам технике и техничких достигнућа (2023):37,
https://hdl.handle.net/21.15107/rcub_technorep_7078 .

TY  - JOUR
AU  - Čutović, Natalija
AU  - Batinić, Petar
AU  - Marković, Tatjana
AU  - Radanović, Dragoja
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Jovanović, Aleksandra A.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7041
AB  - The presented study aimed to optimize polyphenol extraction from Satureja montana L. obtained from the experimental field of the Institute for Medicinal Plants Research ”Dr Josif Pančić”, Serbia, by varying the most important parameters for maceration, solid-to-solvent ratio, and extraction time. The obtained extracts were characterized regarding the total polyphenol content (TPC), total flavonoid content (TFC), antioxidant potential, extraction yield, conductivity, density, surface tension, and viscosity. The TPC and TFC were ~7 to 92 mg GAE (gallic acid equivalent)/g and 3.7 to 10.9 mg CE (catechin equivalent)/g, respectively. The highest extraction yield (86 %) as well as the highest antioxidant activities were obtained for the extracts prepared using a solid-to-solvent ratio of 1 g:50 cm3. On the other hand, the extraction time did not have a significant influence. The highest conductivity was measured in the extract prepared at a 1 g:10 cm3 ratio, while the highest density and surface tension were in the extract prepared at a 1 g:40 cm3 ratio (941 kg m-3 and 29.0 mN m-1, respectively). The highest viscosity was measured in the extract prepared at a 1 g: 20 cm3 ratio (2.89 mPa∙s). Our study shows the possibilities for the production of polyphenol-rich extract of S. montana which might be used in pharmaceutical, food, or cosmetic products.
AB  - Prikazana  studija  je  imala  za  cilj  da  optimizuje  ekstrakciju  polifenola  izbiljkeSatureja  montanaL.  sa oglednog  polja  Instituta  za proučavanjelekovitog bilja „Dr Josif Pančić“, Srbija, variranjem najvažnijih parametara  maceracije,  odnosa biljnog materijala i rastvarača,i  ekstrakcionogvremena.  Dobijeni ekstrakti su okarakterisani u pogleduukupnog sadržaja polifenola (engl.total total polyphenol content, TPC), ukupnog sadržaja flavonoida (engl.total  flavonoid  content,TFC),  antioksidativnog  potencijala, prinosa  ekstrakcije,  provodljivosti,  gustine,  površinskog  napona  i  viskoznosti. Vrednostiukupnog sadržaja polifenola(engl.Total  Polyphenol  Content -TPC)iukupnog sadržaja flavonoida (engl.Total Flavonoid  Content -TFC)su  bile  u  opsezima7,2-92,2  mg ekvivalenta  galne  kiseline  (engl.gallic  acid equivalentGAE) /g i 3,7-10,9 mgekvivalenta katehina(engl.catechinequivalentCE)/g, redom. Najveće antioksidativne  aktivnosti dobijenesu  u  ekstraktima  pripremljenim  u  odnosu biljnog  materijala  i rastvarača1g :50cm-3. Najveći prinos ekstrakcije takođe je dobijenu tom ekstraktu (86,0%), dok vreme ekstrakcije nije imalo značajan uticaj. Najveća  provodljivost  izmerena  je  u  ekstraktu  pripremljenom  u odnosu biljnog materijala i rastvarača1g :10cm-3. Najvećagustina i površinski napon su bili u ekstraktu pripremljenom u odnosu 1g :40cm-3(941 kg m-3i 29,0 mNm-1, redom). Najvećaviskoznostje izmerenau  ekstraktu  pripremljenom  u  odnosu  1g :20cm-3(2,89 mPa∙s). Naša studija pokazuje mogućnosti  za proizvodnju  polifenolima  bogatog  ekstraktabiljkeS.  montanakoji se može koristiti u farmaceutskim, prehrambenim ili kozmetičkim proizvodima.
PB  - Savez hemijskih inženjera Srbije
T2  - Hemijska industrija
T1  - Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts
T1  - Optimizacija procesa ekstrakcije iz biljke Satureja montanal.: fizičkohemijska karakterizacija ekstrakata
EP  - 263
IS  - 4
SP  - 251
VL  - 77
DO  - 10.2298/HEMIND221213020C
ER  - 

@article{
author = "Čutović, Natalija and Batinić, Petar and Marković, Tatjana and Radanović, Dragoja and Marinković, Aleksandar and Bugarski, Branko and Jovanović, Aleksandra A.",
year = "2023",
abstract = "The presented study aimed to optimize polyphenol extraction from Satureja montana L. obtained from the experimental field of the Institute for Medicinal Plants Research ”Dr Josif Pančić”, Serbia, by varying the most important parameters for maceration, solid-to-solvent ratio, and extraction time. The obtained extracts were characterized regarding the total polyphenol content (TPC), total flavonoid content (TFC), antioxidant potential, extraction yield, conductivity, density, surface tension, and viscosity. The TPC and TFC were ~7 to 92 mg GAE (gallic acid equivalent)/g and 3.7 to 10.9 mg CE (catechin equivalent)/g, respectively. The highest extraction yield (86 %) as well as the highest antioxidant activities were obtained for the extracts prepared using a solid-to-solvent ratio of 1 g:50 cm3. On the other hand, the extraction time did not have a significant influence. The highest conductivity was measured in the extract prepared at a 1 g:10 cm3 ratio, while the highest density and surface tension were in the extract prepared at a 1 g:40 cm3 ratio (941 kg m-3 and 29.0 mN m-1, respectively). The highest viscosity was measured in the extract prepared at a 1 g: 20 cm3 ratio (2.89 mPa∙s). Our study shows the possibilities for the production of polyphenol-rich extract of S. montana which might be used in pharmaceutical, food, or cosmetic products., Prikazana  studija  je  imala  za  cilj  da  optimizuje  ekstrakciju  polifenola  izbiljkeSatureja  montanaL.  sa oglednog  polja  Instituta  za proučavanjelekovitog bilja „Dr Josif Pančić“, Srbija, variranjem najvažnijih parametara  maceracije,  odnosa biljnog materijala i rastvarača,i  ekstrakcionogvremena.  Dobijeni ekstrakti su okarakterisani u pogleduukupnog sadržaja polifenola (engl.total total polyphenol content, TPC), ukupnog sadržaja flavonoida (engl.total  flavonoid  content,TFC),  antioksidativnog  potencijala, prinosa  ekstrakcije,  provodljivosti,  gustine,  površinskog  napona  i  viskoznosti. Vrednostiukupnog sadržaja polifenola(engl.Total  Polyphenol  Content -TPC)iukupnog sadržaja flavonoida (engl.Total Flavonoid  Content -TFC)su  bile  u  opsezima7,2-92,2  mg ekvivalenta  galne  kiseline  (engl.gallic  acid equivalentGAE) /g i 3,7-10,9 mgekvivalenta katehina(engl.catechinequivalentCE)/g, redom. Najveće antioksidativne  aktivnosti dobijenesu  u  ekstraktima  pripremljenim  u  odnosu biljnog  materijala  i rastvarača1g :50cm-3. Najveći prinos ekstrakcije takođe je dobijenu tom ekstraktu (86,0%), dok vreme ekstrakcije nije imalo značajan uticaj. Najveća  provodljivost  izmerena  je  u  ekstraktu  pripremljenom  u odnosu biljnog materijala i rastvarača1g :10cm-3. Najvećagustina i površinski napon su bili u ekstraktu pripremljenom u odnosu 1g :40cm-3(941 kg m-3i 29,0 mNm-1, redom). Najvećaviskoznostje izmerenau  ekstraktu  pripremljenom  u  odnosu  1g :20cm-3(2,89 mPa∙s). Naša studija pokazuje mogućnosti  za proizvodnju  polifenolima  bogatog  ekstraktabiljkeS.  montanakoji se može koristiti u farmaceutskim, prehrambenim ili kozmetičkim proizvodima.",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija",
title = "Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts, Optimizacija procesa ekstrakcije iz biljke Satureja montanal.: fizičkohemijska karakterizacija ekstrakata",
pages = "263-251",
number = "4",
volume = "77",
doi = "10.2298/HEMIND221213020C"
}

Čutović, N., Batinić, P., Marković, T., Radanović, D., Marinković, A., Bugarski, B.,& Jovanović, A. A.. (2023). Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts. in Hemijska industrija
Savez hemijskih inženjera Srbije., 77(4), 251-263.
https://doi.org/10.2298/HEMIND221213020C

Čutović N, Batinić P, Marković T, Radanović D, Marinković A, Bugarski B, Jovanović AA. Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts. in Hemijska industrija. 2023;77(4):251-263.
doi:10.2298/HEMIND221213020C .

Čutović, Natalija, Batinić, Petar, Marković, Tatjana, Radanović, Dragoja, Marinković, Aleksandar, Bugarski, Branko, Jovanović, Aleksandra A., "Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts" in Hemijska industrija, 77, no. 4 (2023):251-263,
https://doi.org/10.2298/HEMIND221213020C . .

TY  - JOUR
AU  - Čutović, Natalija
AU  - Marković, Tatjana
AU  - Carević, Tamara
AU  - Stojković, Dejan
AU  - Bugarski, Branko
AU  - Jovanović, Aleksandra A.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7073
AB  - Paeonia tenuifolia L. (steppe peony) petal extract was proficiently encapsulated into liposomes and biopolymer films in the current work, both times utilizing a single-step procedure. The encapsulation efficiency, size of the particles, and index of polydispersity (PDI), as well as the ζ potential of the obtained liposomes were determined, whereas in the case of films, the test included moisture content and mechanical property assessment. Fourier transform infrared spectroscopy (FT-IR) was used to evaluate the chemical composition and existence of numerous interactions in the systems. All the obtained encapsulates were subjected to antibacterial, antifungal and antibiofilm activity testing of the pathogens associated with human skin. The results indicated that the liposomes prepared using Phospholipon had the highest encapsulation efficiency (72.04%), making them the most favorable ones in the release study as well. The biological assays also revealed that Phospholipon was the most beneficial phospholipid mixture for the preparation of liposomes, whereas the film containing these liposomes did not have the ability to inhibit pathogen growth, making the double encapsulation of P. tenuifolia L. petal extract needless. These findings may be a first step toward the potential use of steppe peony extract-loaded films and liposomes in pharmaceutical and cosmetical industries.
PB  - MDPI
T2  - Pharmaceutics
T1  - Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential
IS  - 12
SP  - 2742
VL  - 15
DO  - 10.3390/pharmaceutics15122742
ER  - 

@article{
author = "Čutović, Natalija and Marković, Tatjana and Carević, Tamara and Stojković, Dejan and Bugarski, Branko and Jovanović, Aleksandra A.",
year = "2023",
abstract = "Paeonia tenuifolia L. (steppe peony) petal extract was proficiently encapsulated into liposomes and biopolymer films in the current work, both times utilizing a single-step procedure. The encapsulation efficiency, size of the particles, and index of polydispersity (PDI), as well as the ζ potential of the obtained liposomes were determined, whereas in the case of films, the test included moisture content and mechanical property assessment. Fourier transform infrared spectroscopy (FT-IR) was used to evaluate the chemical composition and existence of numerous interactions in the systems. All the obtained encapsulates were subjected to antibacterial, antifungal and antibiofilm activity testing of the pathogens associated with human skin. The results indicated that the liposomes prepared using Phospholipon had the highest encapsulation efficiency (72.04%), making them the most favorable ones in the release study as well. The biological assays also revealed that Phospholipon was the most beneficial phospholipid mixture for the preparation of liposomes, whereas the film containing these liposomes did not have the ability to inhibit pathogen growth, making the double encapsulation of P. tenuifolia L. petal extract needless. These findings may be a first step toward the potential use of steppe peony extract-loaded films and liposomes in pharmaceutical and cosmetical industries.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential",
number = "12",
pages = "2742",
volume = "15",
doi = "10.3390/pharmaceutics15122742"
}

Čutović, N., Marković, T., Carević, T., Stojković, D., Bugarski, B.,& Jovanović, A. A.. (2023). Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential. in Pharmaceutics
MDPI., 15(12), 2742.
https://doi.org/10.3390/pharmaceutics15122742

Čutović N, Marković T, Carević T, Stojković D, Bugarski B, Jovanović AA. Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential. in Pharmaceutics. 2023;15(12):2742.
doi:10.3390/pharmaceutics15122742 .

Čutović, Natalija, Marković, Tatjana, Carević, Tamara, Stojković, Dejan, Bugarski, Branko, Jovanović, Aleksandra A., "Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential" in Pharmaceutics, 15, no. 12 (2023):2742,
https://doi.org/10.3390/pharmaceutics15122742 . .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Petrović, Predrag
AU  - Volić, Mina
AU  - Obradović, Nataša
AU  - Balanč, Bojana
AU  - Pravilović, Radoslava
AU  - Bugarski, Branko
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6076
AB  - In the present study, Serpylli herba's waste extracts were prepared using 50% ethanol (pH 6) and 50%
ethanol with hydrochloric acid (pH 1.2), using maceration (25 °C) and heat-assisted extraction (HAE,
80 °C). The best extraction conditions were determined via analyzing total polyphenol (TPC) and
flavonoid contents (TFC), antioxidant capacity, extraction yield, zeta potential, density, and surface
tension. In maceration and HAE, TPC were 1.18±0.06 and 1.43±0.05 mg GAE/mL (pH 6), and
0.69±0.05 and 0.86±0.02 mg GAE/mL (pH 1.2). The same trend is noticed in the case of TFC:
0.33±0.01 and 0.42±0.02 mg CE/mL (pH 6) and 0.24±0.01 and 0.27±0.01 mg CE/mL (pH 1.2). ABTS
radical scavenging activity was 775.0±3.5 and 623.8±5.1 mmol Trolox/mL (pH 6), and 269.2±3.1
and 327.9±7.7 mmol Trolox/mL (pH 1.2). In DPPH assay, IC50 was 1.64±0.04 and 1.48±0.03 mg/mL
(pH 6), and 7.59±0.07 and 5.11±0.09 mg/mL (pH 1.2). Extraction yield was 0.541±0.049 and
0.583±0.019% (pH 6), and 0.938±0.057 and 0.889±0.060% (pH 1.2). Zeta potential was -2.58±0.02
and -2.08±0.10 mV (pH 6), and 1.49±0.37 and 1.08±0.09 mV (pH 1.2). Density was 0.945±0.001
and 0.949±0.001 g/mL (pH 6), and 1.008±0.001 and 1.010±0.001 g/mL (pH 1.2). Surface tension
was 29.2±0.3 and 30.5±1.0 mN/m (pH 6), and 32.6±2.3 and 36.5±2.3 mN/m (pH 1.2). The best
properties were determined in the extract obtained in HAE at pH 6. The aim of this study is to provide
evidence of the physico-chemical properties of Serpylli herba's waste extracts that can add value and
improve the quality of existing food, pharmaceutical, and cosmetic products.
PB  - University in Banjaluka, Faculty of Technology
C3  - XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA
T1  - The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts
EP  - 104
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6076
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Petrović, Predrag and Volić, Mina and Obradović, Nataša and Balanč, Bojana and Pravilović, Radoslava and Bugarski, Branko",
year = "2023",
abstract = "In the present study, Serpylli herba's waste extracts were prepared using 50% ethanol (pH 6) and 50%
ethanol with hydrochloric acid (pH 1.2), using maceration (25 °C) and heat-assisted extraction (HAE,
80 °C). The best extraction conditions were determined via analyzing total polyphenol (TPC) and
flavonoid contents (TFC), antioxidant capacity, extraction yield, zeta potential, density, and surface
tension. In maceration and HAE, TPC were 1.18±0.06 and 1.43±0.05 mg GAE/mL (pH 6), and
0.69±0.05 and 0.86±0.02 mg GAE/mL (pH 1.2). The same trend is noticed in the case of TFC:
0.33±0.01 and 0.42±0.02 mg CE/mL (pH 6) and 0.24±0.01 and 0.27±0.01 mg CE/mL (pH 1.2). ABTS
radical scavenging activity was 775.0±3.5 and 623.8±5.1 mmol Trolox/mL (pH 6), and 269.2±3.1
and 327.9±7.7 mmol Trolox/mL (pH 1.2). In DPPH assay, IC50 was 1.64±0.04 and 1.48±0.03 mg/mL
(pH 6), and 7.59±0.07 and 5.11±0.09 mg/mL (pH 1.2). Extraction yield was 0.541±0.049 and
0.583±0.019% (pH 6), and 0.938±0.057 and 0.889±0.060% (pH 1.2). Zeta potential was -2.58±0.02
and -2.08±0.10 mV (pH 6), and 1.49±0.37 and 1.08±0.09 mV (pH 1.2). Density was 0.945±0.001
and 0.949±0.001 g/mL (pH 6), and 1.008±0.001 and 1.010±0.001 g/mL (pH 1.2). Surface tension
was 29.2±0.3 and 30.5±1.0 mN/m (pH 6), and 32.6±2.3 and 36.5±2.3 mN/m (pH 1.2). The best
properties were determined in the extract obtained in HAE at pH 6. The aim of this study is to provide
evidence of the physico-chemical properties of Serpylli herba's waste extracts that can add value and
improve the quality of existing food, pharmaceutical, and cosmetic products.",
publisher = "University in Banjaluka, Faculty of Technology",
journal = "XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA",
title = "The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts",
pages = "104-96",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6076"
}

Jovanović, A. A., Petrović, P., Volić, M., Obradović, N., Balanč, B., Pravilović, R.,& Bugarski, B.. (2023). The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts. in XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA
University in Banjaluka, Faculty of Technology., 96-104.
https://hdl.handle.net/21.15107/rcub_technorep_6076

Jovanović AA, Petrović P, Volić M, Obradović N, Balanč B, Pravilović R, Bugarski B. The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts. in XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA. 2023;:96-104.
https://hdl.handle.net/21.15107/rcub_technorep_6076 .

Jovanović, Aleksandra A., Petrović, Predrag, Volić, Mina, Obradović, Nataša, Balanč, Bojana, Pravilović, Radoslava, Bugarski, Branko, "The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts" in XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA (2023):96-104,
https://hdl.handle.net/21.15107/rcub_technorep_6076 .

TY  - CHAP
AU  - Gómez-Mascaraque, Laura G.
AU  - Balanč, Bojana
AU  - Đorđević, Verica
AU  - Bugarski, Branko
AU  - Trifković, Kata
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6130
AB  - Demand for functional food that offers health benefits beyond basic nutrition is increasing. However, challenges in development of nutritionally-enriched food are often limiting its wider production. Generally, the bioactivity losses during food processing, the immiscibility of active ingredients with the food matrix, the change in sensorial properties of the food products and the low bioavailability of some of the active ingredients are challenges faced when developing functional foods. This chapter reviews the state-of-the-art in microencapsulation techniques that can help to overcome aforementioned difficulties. Microencapsulation of sensitive food ingredients provides not only protection against detrimental effects of temperature, light, pH or oxygen, but also delayed/controlled release of actives, masking of unpleasant flavors and it can impact food structure. The most applicable microencapsulation techniques for food purposes are described, and the main advantages and current challenges, as well as the latest advances in application of these technologies are discussed. The microencapsulation techniques included in this chapter are spray and freeze drying, electrospraying, spray-chilling and spray-cooling, emulsification techniques, fluidized-bed coating, supercritical fluid techniques, production of microgels, coacervation, liposomal techniques and inclusion complexation.
PB  - CRC Press
T2  - Encapsulation in Food Processing and Fermentation
T1  - Encapsulation Techniques for Food Purposes
EP  - 80
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6130
ER  - 

@inbook{
author = "Gómez-Mascaraque, Laura G. and Balanč, Bojana and Đorđević, Verica and Bugarski, Branko and Trifković, Kata",
year = "2022",
abstract = "Demand for functional food that offers health benefits beyond basic nutrition is increasing. However, challenges in development of nutritionally-enriched food are often limiting its wider production. Generally, the bioactivity losses during food processing, the immiscibility of active ingredients with the food matrix, the change in sensorial properties of the food products and the low bioavailability of some of the active ingredients are challenges faced when developing functional foods. This chapter reviews the state-of-the-art in microencapsulation techniques that can help to overcome aforementioned difficulties. Microencapsulation of sensitive food ingredients provides not only protection against detrimental effects of temperature, light, pH or oxygen, but also delayed/controlled release of actives, masking of unpleasant flavors and it can impact food structure. The most applicable microencapsulation techniques for food purposes are described, and the main advantages and current challenges, as well as the latest advances in application of these technologies are discussed. The microencapsulation techniques included in this chapter are spray and freeze drying, electrospraying, spray-chilling and spray-cooling, emulsification techniques, fluidized-bed coating, supercritical fluid techniques, production of microgels, coacervation, liposomal techniques and inclusion complexation.",
publisher = "CRC Press",
journal = "Encapsulation in Food Processing and Fermentation",
booktitle = "Encapsulation Techniques for Food Purposes",
pages = "80-37",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6130"
}

Gómez-Mascaraque, L. G., Balanč, B., Đorđević, V., Bugarski, B.,& Trifković, K.. (2022). Encapsulation Techniques for Food Purposes. in Encapsulation in Food Processing and Fermentation
CRC Press., 37-80.
https://hdl.handle.net/21.15107/rcub_technorep_6130

Gómez-Mascaraque LG, Balanč B, Đorđević V, Bugarski B, Trifković K. Encapsulation Techniques for Food Purposes. in Encapsulation in Food Processing and Fermentation. 2022;:37-80.
https://hdl.handle.net/21.15107/rcub_technorep_6130 .

Gómez-Mascaraque, Laura G., Balanč, Bojana, Đorđević, Verica, Bugarski, Branko, Trifković, Kata, "Encapsulation Techniques for Food Purposes" in Encapsulation in Food Processing and Fermentation (2022):37-80,
https://hdl.handle.net/21.15107/rcub_technorep_6130 .

TY  - CONF
AU  - Pavlović, Neda
AU  - Mijalković, Jelena
AU  - Đorđević, Verica
AU  - Bugarski, Branko
AU  - Knežević-Jugović, Zorica
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6250
AB  - Liposomes have been researched as carriers for encapsulation of
bioactive protein hydrolysate, despite low stability which makes them difficult to 
use. Using cholesterol or octadecylamine as lipid membrane stabilizers, soy protein 
hydrolysate is effectively encapsulated in liposomes created with phospholipon.
Presence of both stabilizers concurrently resulted the highest efficiency 49.7%, while 
increasing the phospholipon mass had no effect. Particle size was raised (265 to 335 
nm) as a result of the octadecylamine addition, and zeta potential was positive (36.7 
mV), as compared to the cholesterol addition (-25.1 mV). Liposomes enabled 
extended hydrolysate release with retained its bioactivity.
AB  - Lipozomi su dugo proučavani nosači za inkapsulaciju bioaktivnih proteinskih 
hidrolizata, uprkos niskoj stabilnost koja predstavlja problem za širu primenu.
Koristeći stabilizatore holesterol i oktadecilamin, proteinski hidrolizat soje je 
uspešno inkapsuliran u lipozome sačinjene od fosfolipona. Prisustvo oba 
stabilizatora istovremeno rezultiralo je najvećom efikasnišću 49,7 %, dok povećanje 
početne mase fosfolipona nije imalo uticaja na efikasnot. Veličina lipozoma je 
povećana (sa 265 na 335 nm) kao rezultata dodavanja oktadecilamina, ali je zeta 
potencilaj pozitivan (36,7 mV) u poređenju sa dodatkom holesterola (-25,1 mV). 
Pripremljeni lipozomi omogućili su produženo oslobađanje hidrolizata soje uz 
očuvanje njegove bioaktivnosti.
PB  - Kragujevac : Univerzitet, Agronomski fakultet u Čačku, 2022 (Beograd : Birograf Comp)
C3  - Zbornik radova / XXVII Savetovanje o biotehnologiji sa međunarodnim učešćem
T1  - Characteristics of octadecylamine-stabilized liposomes containing soy protein hydrolysates
EP  - 495
SP  - 489
DO  - 10.46793/SBT27.489P
ER  - 

@conference{
author = "Pavlović, Neda and Mijalković, Jelena and Đorđević, Verica and Bugarski, Branko and Knežević-Jugović, Zorica",
year = "2022",
abstract = "Liposomes have been researched as carriers for encapsulation of
bioactive protein hydrolysate, despite low stability which makes them difficult to 
use. Using cholesterol or octadecylamine as lipid membrane stabilizers, soy protein 
hydrolysate is effectively encapsulated in liposomes created with phospholipon.
Presence of both stabilizers concurrently resulted the highest efficiency 49.7%, while 
increasing the phospholipon mass had no effect. Particle size was raised (265 to 335 
nm) as a result of the octadecylamine addition, and zeta potential was positive (36.7 
mV), as compared to the cholesterol addition (-25.1 mV). Liposomes enabled 
extended hydrolysate release with retained its bioactivity., Lipozomi su dugo proučavani nosači za inkapsulaciju bioaktivnih proteinskih 
hidrolizata, uprkos niskoj stabilnost koja predstavlja problem za širu primenu.
Koristeći stabilizatore holesterol i oktadecilamin, proteinski hidrolizat soje je 
uspešno inkapsuliran u lipozome sačinjene od fosfolipona. Prisustvo oba 
stabilizatora istovremeno rezultiralo je najvećom efikasnišću 49,7 %, dok povećanje 
početne mase fosfolipona nije imalo uticaja na efikasnot. Veličina lipozoma je 
povećana (sa 265 na 335 nm) kao rezultata dodavanja oktadecilamina, ali je zeta 
potencilaj pozitivan (36,7 mV) u poređenju sa dodatkom holesterola (-25,1 mV). 
Pripremljeni lipozomi omogućili su produženo oslobađanje hidrolizata soje uz 
očuvanje njegove bioaktivnosti.",
publisher = "Kragujevac : Univerzitet, Agronomski fakultet u Čačku, 2022 (Beograd : Birograf Comp)",
journal = "Zbornik radova / XXVII Savetovanje o biotehnologiji sa međunarodnim učešćem",
title = "Characteristics of octadecylamine-stabilized liposomes containing soy protein hydrolysates",
pages = "495-489",
doi = "10.46793/SBT27.489P"
}

Pavlović, N., Mijalković, J., Đorđević, V., Bugarski, B.,& Knežević-Jugović, Z.. (2022). Characteristics of octadecylamine-stabilized liposomes containing soy protein hydrolysates. in Zbornik radova / XXVII Savetovanje o biotehnologiji sa međunarodnim učešćem
Kragujevac : Univerzitet, Agronomski fakultet u Čačku, 2022 (Beograd : Birograf Comp)., 489-495.
https://doi.org/10.46793/SBT27.489P

Pavlović N, Mijalković J, Đorđević V, Bugarski B, Knežević-Jugović Z. Characteristics of octadecylamine-stabilized liposomes containing soy protein hydrolysates. in Zbornik radova / XXVII Savetovanje o biotehnologiji sa međunarodnim učešćem. 2022;:489-495.
doi:10.46793/SBT27.489P .

Pavlović, Neda, Mijalković, Jelena, Đorđević, Verica, Bugarski, Branko, Knežević-Jugović, Zorica, "Characteristics of octadecylamine-stabilized liposomes containing soy protein hydrolysates" in Zbornik radova / XXVII Savetovanje o biotehnologiji sa međunarodnim učešćem (2022):489-495,
https://doi.org/10.46793/SBT27.489P . .

TY  - CONF
AU  - Pavlović, Neda
AU  - Mijalković, Jelena
AU  - Šekuljica, Nataša
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Bugarski, Branko
AU  - Knežević-Jugović, Zorica
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6251
AB  - Nowadays, the necessity for dietary nutrient encapsulation emerges largely since the action of digestive enzymes causes the loss of structural integrity and function of nutrients. Proteins have high nutritional value and thus are considered as the most nutritionally beneficial carriers; moreover, due to their versatility, proteins can be generated in a variety of structures (nanoparticles, nanofilms, nanofibers, coacervates, and hydrogels), allowing for the delivery of hydrophobic or hydrophilic nutrients. Proteins of vegetable origin have broader application potential over proteins of animal sources. Thus, the modern research focuses on waste leaf biomass, such as pumpkin leaves, as a rich source of plant proteins, specifically the most abundant protein in the world, ribulose-1,5-bisphosphate carboxylase-oxygenase, RuBisCo protein. 
The aim of this work was to isolate the water-soluble proteins, mostly RuBisCO protein, from pumpkin leaves in order to utilize it as a carrier for folic acid (FA) encapsulation. The resulting protein leaf concentrate was lyophilized and used as such to form nanoparticles. Leaf protein nanoparticles were prepared by employing a Ca-induced cold gelation method. 
Results and conclusions. The results showed that the Ca-induced protein nanoparticles have a small particle size (58–208 nm), polydispersity index (PDI) of 0.241 to 0.377, and negative zeta potential (-16.2 to -23.9 mV). Protein leaf nanoparticles exhibited uniform unimodal size distribution and spherical shape with a unique honeycomb-like core structure. Nanoparticle characteristics including size, surface charge and hydrophobicity could be adjustable by changing calcium chloride concentration from 2 to 10 mM and environmental pH (7 to 9). All manufactured nanoparticles demonstrated good stability over seven days, with no significant changes in zeta potential or formation agglomerates. Release studies showed that the leaf protein nanoparticles with FA, encapsulation efficiency amounted 40.2%, were resistant to pepsin and low pH in simulated gastric fluid (10% of initial FA amount was realized), but released the encapsulated FA in simulated intestinal conditions was enriched, even another 50% was achieved.  
Pumpkin leaf protein nanoparticles have a promising possibility for bioactive ingredient delivery owing to the characteristics described above. These findings would be of great importance for the development of food-grade nanoparticles suitable for the formulations of functional foods.
PB  - Zagreb, Croatia : Croatian Society of Food Technologists, Biotechnologists and Nutritionists
C3  - Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity
T1  - Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins
EP  - 142
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6251
ER  - 

@conference{
author = "Pavlović, Neda and Mijalković, Jelena and Šekuljica, Nataša and Petrović, Predrag and Đorđević, Verica and Bugarski, Branko and Knežević-Jugović, Zorica",
year = "2022",
abstract = "Nowadays, the necessity for dietary nutrient encapsulation emerges largely since the action of digestive enzymes causes the loss of structural integrity and function of nutrients. Proteins have high nutritional value and thus are considered as the most nutritionally beneficial carriers; moreover, due to their versatility, proteins can be generated in a variety of structures (nanoparticles, nanofilms, nanofibers, coacervates, and hydrogels), allowing for the delivery of hydrophobic or hydrophilic nutrients. Proteins of vegetable origin have broader application potential over proteins of animal sources. Thus, the modern research focuses on waste leaf biomass, such as pumpkin leaves, as a rich source of plant proteins, specifically the most abundant protein in the world, ribulose-1,5-bisphosphate carboxylase-oxygenase, RuBisCo protein. 
The aim of this work was to isolate the water-soluble proteins, mostly RuBisCO protein, from pumpkin leaves in order to utilize it as a carrier for folic acid (FA) encapsulation. The resulting protein leaf concentrate was lyophilized and used as such to form nanoparticles. Leaf protein nanoparticles were prepared by employing a Ca-induced cold gelation method. 
Results and conclusions. The results showed that the Ca-induced protein nanoparticles have a small particle size (58–208 nm), polydispersity index (PDI) of 0.241 to 0.377, and negative zeta potential (-16.2 to -23.9 mV). Protein leaf nanoparticles exhibited uniform unimodal size distribution and spherical shape with a unique honeycomb-like core structure. Nanoparticle characteristics including size, surface charge and hydrophobicity could be adjustable by changing calcium chloride concentration from 2 to 10 mM and environmental pH (7 to 9). All manufactured nanoparticles demonstrated good stability over seven days, with no significant changes in zeta potential or formation agglomerates. Release studies showed that the leaf protein nanoparticles with FA, encapsulation efficiency amounted 40.2%, were resistant to pepsin and low pH in simulated gastric fluid (10% of initial FA amount was realized), but released the encapsulated FA in simulated intestinal conditions was enriched, even another 50% was achieved.  
Pumpkin leaf protein nanoparticles have a promising possibility for bioactive ingredient delivery owing to the characteristics described above. These findings would be of great importance for the development of food-grade nanoparticles suitable for the formulations of functional foods.",
publisher = "Zagreb, Croatia : Croatian Society of Food Technologists, Biotechnologists and Nutritionists",
journal = "Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity",
title = "Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins",
pages = "142-141",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6251"
}

Pavlović, N., Mijalković, J., Šekuljica, N., Petrović, P., Đorđević, V., Bugarski, B.,& Knežević-Jugović, Z.. (2022). Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins. in Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity
Zagreb, Croatia : Croatian Society of Food Technologists, Biotechnologists and Nutritionists., 141-142.
https://hdl.handle.net/21.15107/rcub_technorep_6251

Pavlović N, Mijalković J, Šekuljica N, Petrović P, Đorđević V, Bugarski B, Knežević-Jugović Z. Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins. in Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity. 2022;:141-142.
https://hdl.handle.net/21.15107/rcub_technorep_6251 .

Pavlović, Neda, Mijalković, Jelena, Šekuljica, Nataša, Petrović, Predrag, Đorđević, Verica, Bugarski, Branko, Knežević-Jugović, Zorica, "Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins" in Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity (2022):141-142,
https://hdl.handle.net/21.15107/rcub_technorep_6251 .

TY  - JOUR
AU  - Bošković, Stefan A.
AU  - Karač, Aleksandar
AU  - Vrhovac, Slobodan B.
AU  - Aleksandar, Belić
AU  - Bugarski, Branko
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6619
AB  - The electrohydrodynamics' (EHD) perfect dielectric model was added into computational fluid dynamics (CFD) software OpenFOAM® in order to improve its usability for the EHD field and specifically for the mentioned model. Based on the investigated literature, it can be said that this is the most complete implementation of the said model. Two sets of numerical simulations with two different fluids are presented and analyzed. One set is one-dimensional. The other set is with a drop of one fluid surrounded by other fluid. Oscillations can be observed with certain expressions or calculation strategies for the electrostrictive force, and used for disregarding them. Results that are closer to analytical predictions can be obtained by using appropriate expression for the dielectric force. The electrostrictive force was implemented not only for nonpolar, but also for polar fluids, and it is shown that it might significantly influence the drop deformation. Calculated and analytically predicted drop deformations were close or comparable even up to around 0.25, what is significantly higher and different from a previous study made by other authors. Different expressions for the electric permittivity and usage of limiters for volume fractions were investigated. Conclusions from this paper can be transferred to more complicated models.
PB  - University of East Sarajevo, Faculty of Technology Zvornik
T2  - Journal of Engineering & Processing Management
T1  - Implementation of the electrohydrodynamics' perfect dielectric model in OpenFOAM®
EP  - 75
IS  - 2
SP  - 66
VL  - 14
DO  - 10.7251/JEPM2202066B
ER  - 

@article{
author = "Bošković, Stefan A. and Karač, Aleksandar and Vrhovac, Slobodan B. and Aleksandar, Belić and Bugarski, Branko",
year = "2022",
abstract = "The electrohydrodynamics' (EHD) perfect dielectric model was added into computational fluid dynamics (CFD) software OpenFOAM® in order to improve its usability for the EHD field and specifically for the mentioned model. Based on the investigated literature, it can be said that this is the most complete implementation of the said model. Two sets of numerical simulations with two different fluids are presented and analyzed. One set is one-dimensional. The other set is with a drop of one fluid surrounded by other fluid. Oscillations can be observed with certain expressions or calculation strategies for the electrostrictive force, and used for disregarding them. Results that are closer to analytical predictions can be obtained by using appropriate expression for the dielectric force. The electrostrictive force was implemented not only for nonpolar, but also for polar fluids, and it is shown that it might significantly influence the drop deformation. Calculated and analytically predicted drop deformations were close or comparable even up to around 0.25, what is significantly higher and different from a previous study made by other authors. Different expressions for the electric permittivity and usage of limiters for volume fractions were investigated. Conclusions from this paper can be transferred to more complicated models.",
publisher = "University of East Sarajevo, Faculty of Technology Zvornik",
journal = "Journal of Engineering & Processing Management",
title = "Implementation of the electrohydrodynamics' perfect dielectric model in OpenFOAM®",
pages = "75-66",
number = "2",
volume = "14",
doi = "10.7251/JEPM2202066B"
}

Bošković, S. A., Karač, A., Vrhovac, S. B., Aleksandar, B.,& Bugarski, B.. (2022). Implementation of the electrohydrodynamics' perfect dielectric model in OpenFOAM®. in Journal of Engineering & Processing Management
University of East Sarajevo, Faculty of Technology Zvornik., 14(2), 66-75.
https://doi.org/10.7251/JEPM2202066B

Bošković SA, Karač A, Vrhovac SB, Aleksandar B, Bugarski B. Implementation of the electrohydrodynamics' perfect dielectric model in OpenFOAM®. in Journal of Engineering & Processing Management. 2022;14(2):66-75.
doi:10.7251/JEPM2202066B .

Bošković, Stefan A., Karač, Aleksandar, Vrhovac, Slobodan B., Aleksandar, Belić, Bugarski, Branko, "Implementation of the electrohydrodynamics' perfect dielectric model in OpenFOAM®" in Journal of Engineering & Processing Management, 14, no. 2 (2022):66-75,
https://doi.org/10.7251/JEPM2202066B . .

TY  - CHAP
AU  - Đorđević, Verica
AU  - Balanč, Bojana
AU  - Kalušević, Ana
AU  - Volić, Mina
AU  - Obradović, Nataša
AU  - Petrović, Predrag
AU  - Bugarski, Branko
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6128
AB  - The controlled active molecule-delivery technology has progressed over the last decades, with the development of sustained release systems for application in food sector. It is useful to control the rate and extent of food digestion in different regions of the gastrointestinal tract (GIT) and release of an active compound with the ultimate goal of controlling bioavailability and satiety of blood levels. The nature, molecular weight and concentration of a carrier material, as well as the lipophilic/hydrophilic character of the loaded active component, its concentration, interfacial properties and the particle size distribution are some of the key factors that determine the delivery performance of these systems. pH variation, temperature change, variation of the redox potential and enzyme activity are stimuli which most often induce the release of an active molecule in a medium. In order to develop food additives with controlled release, their processing in the gastrointestinal tract has to be understood; therefore, a general description of the digestive system is given and associated to the currently used in vitro experimental models.

This chapter aims to give a useful overview on the gastrointestinal fate of the most frequently used carrier materials and structures of food-grade delivery systems. At the end, mathematical models most useful in fitting to the experimental data of release profiles and resolving the mechanisms implied in controlled release are summarized.
PB  - CRC Press
T2  - Encapsulation in Food Processing and Fermentation
T1  - Controlled Release Systems for Food Application
EP  - 171
SP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6128
ER  - 

@inbook{
author = "Đorđević, Verica and Balanč, Bojana and Kalušević, Ana and Volić, Mina and Obradović, Nataša and Petrović, Predrag and Bugarski, Branko",
year = "2022",
abstract = "The controlled active molecule-delivery technology has progressed over the last decades, with the development of sustained release systems for application in food sector. It is useful to control the rate and extent of food digestion in different regions of the gastrointestinal tract (GIT) and release of an active compound with the ultimate goal of controlling bioavailability and satiety of blood levels. The nature, molecular weight and concentration of a carrier material, as well as the lipophilic/hydrophilic character of the loaded active component, its concentration, interfacial properties and the particle size distribution are some of the key factors that determine the delivery performance of these systems. pH variation, temperature change, variation of the redox potential and enzyme activity are stimuli which most often induce the release of an active molecule in a medium. In order to develop food additives with controlled release, their processing in the gastrointestinal tract has to be understood; therefore, a general description of the digestive system is given and associated to the currently used in vitro experimental models.

This chapter aims to give a useful overview on the gastrointestinal fate of the most frequently used carrier materials and structures of food-grade delivery systems. At the end, mathematical models most useful in fitting to the experimental data of release profiles and resolving the mechanisms implied in controlled release are summarized.",
publisher = "CRC Press",
journal = "Encapsulation in Food Processing and Fermentation",
booktitle = "Controlled Release Systems for Food Application",
pages = "171-142",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6128"
}

Đorđević, V., Balanč, B., Kalušević, A., Volić, M., Obradović, N., Petrović, P.,& Bugarski, B.. (2022). Controlled Release Systems for Food Application. in Encapsulation in Food Processing and Fermentation
CRC Press., 142-171.
https://hdl.handle.net/21.15107/rcub_technorep_6128

Đorđević V, Balanč B, Kalušević A, Volić M, Obradović N, Petrović P, Bugarski B. Controlled Release Systems for Food Application. in Encapsulation in Food Processing and Fermentation. 2022;:142-171.
https://hdl.handle.net/21.15107/rcub_technorep_6128 .

Đorđević, Verica, Balanč, Bojana, Kalušević, Ana, Volić, Mina, Obradović, Nataša, Petrović, Predrag, Bugarski, Branko, "Controlled Release Systems for Food Application" in Encapsulation in Food Processing and Fermentation (2022):142-171,
https://hdl.handle.net/21.15107/rcub_technorep_6128 .

TY  - CONF
AU  - Obradović, Nataša
AU  - Volić, Mina
AU  - Jovanović, Aleksandra
AU  - Krunić, Tanja
AU  - Nedović, Viktor
AU  - Rakin, Marica
AU  - Bugarski, Branko
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6446
AB  - Starter cultures are essential components of fermented foods with the potential to enhance
preservation, improve nutritional quality, and modify the sensory qualities of the products. The
study characterized the microcarriers with the probiotic starter culture which could be used for
the preparation of functional whey-based products. The culture was encapsulated using the
spray-drying technique and implemented in the whey-based medium. The protective effect of
alginate-whey carriers on the microencapsulated dairy starter culture (Lactoferm ABY 6)
during the spray-drying, fermentation, and storage time were analyzed. The production yield,
encapsulation efficiency, solubility, particle size, and moisture content were determined after
the microencapsulation process. The SEM analysis was used for the characterization of powder
and carrier surface morphology. The encapsulation efficiency and powder solubility were
75,8±0,09 % and 86,0±1,62 %, respectively. The cell viability in the powders and beverages
was monitored for 28 days. The results showed that the microencapsulation process improved
cell viability (> 9,08 log10CFU/mL) during the fermentation process. Moreover,
microencapsulated probiotic cells have a higher survival rate in beverages in comparison with
free cells in storage time. The application of this type of encapsulation technique showed
potential regarding cell preservation in the production and storage of functional beverages.
PB  - Novi Sad : Faculty of Technology
C3  - Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad
T1  - Design and characterization of spray-dried microcarriers for preparation of functional wheybased beverages
SP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6446
ER  - 

@conference{
author = "Obradović, Nataša and Volić, Mina and Jovanović, Aleksandra and Krunić, Tanja and Nedović, Viktor and Rakin, Marica and Bugarski, Branko",
year = "2022",
abstract = "Starter cultures are essential components of fermented foods with the potential to enhance
preservation, improve nutritional quality, and modify the sensory qualities of the products. The
study characterized the microcarriers with the probiotic starter culture which could be used for
the preparation of functional whey-based products. The culture was encapsulated using the
spray-drying technique and implemented in the whey-based medium. The protective effect of
alginate-whey carriers on the microencapsulated dairy starter culture (Lactoferm ABY 6)
during the spray-drying, fermentation, and storage time were analyzed. The production yield,
encapsulation efficiency, solubility, particle size, and moisture content were determined after
the microencapsulation process. The SEM analysis was used for the characterization of powder
and carrier surface morphology. The encapsulation efficiency and powder solubility were
75,8±0,09 % and 86,0±1,62 %, respectively. The cell viability in the powders and beverages
was monitored for 28 days. The results showed that the microencapsulation process improved
cell viability (> 9,08 log10CFU/mL) during the fermentation process. Moreover,
microencapsulated probiotic cells have a higher survival rate in beverages in comparison with
free cells in storage time. The application of this type of encapsulation technique showed
potential regarding cell preservation in the production and storage of functional beverages.",
publisher = "Novi Sad : Faculty of Technology",
journal = "Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad",
title = "Design and characterization of spray-dried microcarriers for preparation of functional wheybased beverages",
pages = "35",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6446"
}

Obradović, N., Volić, M., Jovanović, A., Krunić, T., Nedović, V., Rakin, M.,& Bugarski, B.. (2022). Design and characterization of spray-dried microcarriers for preparation of functional wheybased beverages. in Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad
Novi Sad : Faculty of Technology., 35.
https://hdl.handle.net/21.15107/rcub_technorep_6446

Obradović N, Volić M, Jovanović A, Krunić T, Nedović V, Rakin M, Bugarski B. Design and characterization of spray-dried microcarriers for preparation of functional wheybased beverages. in Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad. 2022;:35.
https://hdl.handle.net/21.15107/rcub_technorep_6446 .

Obradović, Nataša, Volić, Mina, Jovanović, Aleksandra, Krunić, Tanja, Nedović, Viktor, Rakin, Marica, Bugarski, Branko, "Design and characterization of spray-dried microcarriers for preparation of functional wheybased beverages" in Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad (2022):35,
https://hdl.handle.net/21.15107/rcub_technorep_6446 .

TY  - JOUR
AU  - Obradović, Nataša
AU  - Volić, Mina
AU  - Nedović, Viktor
AU  - Rakin, Marica
AU  - Bugarski, Branko
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6031
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5035
AB  - The production of functional beverages has gained more attention because of growing consumers' interest in personal health. The process of carrier production and characterization of a novel type of protein-carbohydrate carriers for functional beverages with a focus on cell protection during fermentation, gastrointestinal, and storage conditions are shown in this study. Probiotic starter culture was microencapsulated using spray and freeze-drying techniques and then implemented in whey-based beverages. Encapsulation materials used are whey protein concentrate, whey, and sodium alginate. The encapsulation efficiency results were over 76.8% and 81.4%, after spray and freeze-drying respectively, depending on the carrier formulation. The culture in freeze-dried carriers reached a higher survival rate (>7.5 log10(CFUg−1)) than one's encapsulated by spray-drying technique during storage. Microencapsulation of the culture significantly improved cell viability in gastrointestinal and storage conditions compared with free cells. Usage of both encapsulation techniques showed potential regarding cell preservation in production and storage.
PB  - Elsevier Ltd.
T2  - Journal of Food Engineering
T1  - Microencapsulation of probiotic starter culture in protein–carbohydrate carriers using spray and freeze-drying processes: Implementation in whey-based beverages
SP  - 110948
VL  - 321
DO  - 10.1016/j.jfoodeng.2022.110948
ER  - 

@article{
author = "Obradović, Nataša and Volić, Mina and Nedović, Viktor and Rakin, Marica and Bugarski, Branko",
year = "2022",
abstract = "The production of functional beverages has gained more attention because of growing consumers' interest in personal health. The process of carrier production and characterization of a novel type of protein-carbohydrate carriers for functional beverages with a focus on cell protection during fermentation, gastrointestinal, and storage conditions are shown in this study. Probiotic starter culture was microencapsulated using spray and freeze-drying techniques and then implemented in whey-based beverages. Encapsulation materials used are whey protein concentrate, whey, and sodium alginate. The encapsulation efficiency results were over 76.8% and 81.4%, after spray and freeze-drying respectively, depending on the carrier formulation. The culture in freeze-dried carriers reached a higher survival rate (>7.5 log10(CFUg−1)) than one's encapsulated by spray-drying technique during storage. Microencapsulation of the culture significantly improved cell viability in gastrointestinal and storage conditions compared with free cells. Usage of both encapsulation techniques showed potential regarding cell preservation in production and storage.",
publisher = "Elsevier Ltd.",
journal = "Journal of Food Engineering",
title = "Microencapsulation of probiotic starter culture in protein–carbohydrate carriers using spray and freeze-drying processes: Implementation in whey-based beverages",
pages = "110948",
volume = "321",
doi = "10.1016/j.jfoodeng.2022.110948"
}

Obradović, N., Volić, M., Nedović, V., Rakin, M.,& Bugarski, B.. (2022). Microencapsulation of probiotic starter culture in protein–carbohydrate carriers using spray and freeze-drying processes: Implementation in whey-based beverages. in Journal of Food Engineering
Elsevier Ltd.., 321, 110948.
https://doi.org/10.1016/j.jfoodeng.2022.110948

Obradović N, Volić M, Nedović V, Rakin M, Bugarski B. Microencapsulation of probiotic starter culture in protein–carbohydrate carriers using spray and freeze-drying processes: Implementation in whey-based beverages. in Journal of Food Engineering. 2022;321:110948.
doi:10.1016/j.jfoodeng.2022.110948 .

Obradović, Nataša, Volić, Mina, Nedović, Viktor, Rakin, Marica, Bugarski, Branko, "Microencapsulation of probiotic starter culture in protein–carbohydrate carriers using spray and freeze-drying processes: Implementation in whey-based beverages" in Journal of Food Engineering, 321 (2022):110948,
https://doi.org/10.1016/j.jfoodeng.2022.110948 . .

TY  - JOUR
AU  - Salević, Ana
AU  - Stojanović, Dušica
AU  - Lević, Steva
AU  - Pantić, Milena
AU  - Đorđević, Verica
AU  - Pešić, Radojica
AU  - Bugarski, Branko
AU  - Pavlović, Vladimir
AU  - Uskoković, Petar
AU  - Nedović, Viktor
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5008
AB  - In this study, in order to develop zein-based, edible, functional food-contact materials in different forms incorporating sage extract (10, 20, and 30%), solvent casting and electrospinning were employed. The study aimed to assess the effects of the applied techniques and the extract's incorporation on the materials' properties. The solvent casting generated continuous and compact films, where the extract's incorporation provided more homogenous surfaces. The electrospinning resulted in non-woven mats composed of ribbon-like fibers in the range of 1.275-1.829 mu m, while the extract's incorporation provided thinner and branched fibers. The results indicated the compatibility between the materials' constituents, and efficient and homogenous extract incorporation within the zein matrices, with more probable interactions occurring during the solvent casting. All of the formulations had a high dry matter content, whereas the mats and the formulations incorporating the extract had higher solubility and swelling in water. The films and mats presented similar DPPH center dot and ABTS(center dot+) radical scavenging abilities, while the influence on Staphylococcus aureus and Salmonella enterica subsp. enterica serovar Typhimurium bacteria, and the growth inhibition, were complex. The antioxidant and antibacterial activity of the materials were more potent after the extract's incorporation. Overall, the results highlight the potential of the developed edible materials for use as food-contact materials with active/bioactive functionality.
PB  - MDPI
T2  - Foods
T1  - The Structuring of Sage (Salvia officinalis L.) Extract-Incorporating Edible Zein-Based Materials with Antioxidant and Antibacterial Functionality by Solvent Casting versus Electrospinning
IS  - 3
SP  - 390
VL  - 11
DO  - 10.3390/foods11030390
ER  - 

@article{
author = "Salević, Ana and Stojanović, Dušica and Lević, Steva and Pantić, Milena and Đorđević, Verica and Pešić, Radojica and Bugarski, Branko and Pavlović, Vladimir and Uskoković, Petar and Nedović, Viktor",
year = "2022",
abstract = "In this study, in order to develop zein-based, edible, functional food-contact materials in different forms incorporating sage extract (10, 20, and 30%), solvent casting and electrospinning were employed. The study aimed to assess the effects of the applied techniques and the extract's incorporation on the materials' properties. The solvent casting generated continuous and compact films, where the extract's incorporation provided more homogenous surfaces. The electrospinning resulted in non-woven mats composed of ribbon-like fibers in the range of 1.275-1.829 mu m, while the extract's incorporation provided thinner and branched fibers. The results indicated the compatibility between the materials' constituents, and efficient and homogenous extract incorporation within the zein matrices, with more probable interactions occurring during the solvent casting. All of the formulations had a high dry matter content, whereas the mats and the formulations incorporating the extract had higher solubility and swelling in water. The films and mats presented similar DPPH center dot and ABTS(center dot+) radical scavenging abilities, while the influence on Staphylococcus aureus and Salmonella enterica subsp. enterica serovar Typhimurium bacteria, and the growth inhibition, were complex. The antioxidant and antibacterial activity of the materials were more potent after the extract's incorporation. Overall, the results highlight the potential of the developed edible materials for use as food-contact materials with active/bioactive functionality.",
publisher = "MDPI",
journal = "Foods",
title = "The Structuring of Sage (Salvia officinalis L.) Extract-Incorporating Edible Zein-Based Materials with Antioxidant and Antibacterial Functionality by Solvent Casting versus Electrospinning",
number = "3",
pages = "390",
volume = "11",
doi = "10.3390/foods11030390"
}

Salević, A., Stojanović, D., Lević, S., Pantić, M., Đorđević, V., Pešić, R., Bugarski, B., Pavlović, V., Uskoković, P.,& Nedović, V.. (2022). The Structuring of Sage (Salvia officinalis L.) Extract-Incorporating Edible Zein-Based Materials with Antioxidant and Antibacterial Functionality by Solvent Casting versus Electrospinning. in Foods
MDPI., 11(3), 390.
https://doi.org/10.3390/foods11030390

Salević A, Stojanović D, Lević S, Pantić M, Đorđević V, Pešić R, Bugarski B, Pavlović V, Uskoković P, Nedović V. The Structuring of Sage (Salvia officinalis L.) Extract-Incorporating Edible Zein-Based Materials with Antioxidant and Antibacterial Functionality by Solvent Casting versus Electrospinning. in Foods. 2022;11(3):390.
doi:10.3390/foods11030390 .

Salević, Ana, Stojanović, Dušica, Lević, Steva, Pantić, Milena, Đorđević, Verica, Pešić, Radojica, Bugarski, Branko, Pavlović, Vladimir, Uskoković, Petar, Nedović, Viktor, "The Structuring of Sage (Salvia officinalis L.) Extract-Incorporating Edible Zein-Based Materials with Antioxidant and Antibacterial Functionality by Solvent Casting versus Electrospinning" in Foods, 11, no. 3 (2022):390,
https://doi.org/10.3390/foods11030390 . .

TY  - JOUR
AU  - Pavlović, Neda
AU  - Mijalković, Jelena
AU  - Đorđević, Verica
AU  - Pecarski, Danijela
AU  - Bugarski, Branko
AU  - Knežević-Jugović, Zorica
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5384
AB  - This study presents the state-of-art research about the assembly of soy proteins in nanocarriers, liposomes, and its design includes different physicochemical strategies and approaches: two-step enzymatic hydrolysis of soy concentrate, hydrolysate encapsulation by using phospholipids and cholesterol, and application of ultrasonication. Achieved results revealed that ultrasonication, together with cholesterol addition into phospholipid layers, improved the stability of nanoliposomes, and a maximum EE value of 60.5 % was obtained. Average size of peptide-loaded nanoliposomes was found to be from 191.1 to 286.7 nm, with a ζ potential of −25.5 to −34.6 mV, and a polydispersity index of 0.250–0.390. Ultrasound-assisted encapsulation process did not lead to a decrease in the antioxidant activity of the trapped peptides. FTIR has indicated an effective hydrophobic interaction between phosphatidylcholine and hydrolysate peptides. TEM and SEM have confirmed the spherical nanocarrier structure and unilamelarity. Prolonged gastrointestinal release and stability of peptides have been enabled by liposome nanocarriers.
PB  - Elsevier Ltd.
T2  - Food Chemistry: X
T1  - Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion
SP  - 100370
VL  - 15
DO  - 10.1016/j.fochx.2022.100370
ER  - 

@article{
author = "Pavlović, Neda and Mijalković, Jelena and Đorđević, Verica and Pecarski, Danijela and Bugarski, Branko and Knežević-Jugović, Zorica",
year = "2022",
abstract = "This study presents the state-of-art research about the assembly of soy proteins in nanocarriers, liposomes, and its design includes different physicochemical strategies and approaches: two-step enzymatic hydrolysis of soy concentrate, hydrolysate encapsulation by using phospholipids and cholesterol, and application of ultrasonication. Achieved results revealed that ultrasonication, together with cholesterol addition into phospholipid layers, improved the stability of nanoliposomes, and a maximum EE value of 60.5 % was obtained. Average size of peptide-loaded nanoliposomes was found to be from 191.1 to 286.7 nm, with a ζ potential of −25.5 to −34.6 mV, and a polydispersity index of 0.250–0.390. Ultrasound-assisted encapsulation process did not lead to a decrease in the antioxidant activity of the trapped peptides. FTIR has indicated an effective hydrophobic interaction between phosphatidylcholine and hydrolysate peptides. TEM and SEM have confirmed the spherical nanocarrier structure and unilamelarity. Prolonged gastrointestinal release and stability of peptides have been enabled by liposome nanocarriers.",
publisher = "Elsevier Ltd.",
journal = "Food Chemistry: X",
title = "Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion",
pages = "100370",
volume = "15",
doi = "10.1016/j.fochx.2022.100370"
}

Pavlović, N., Mijalković, J., Đorđević, V., Pecarski, D., Bugarski, B.,& Knežević-Jugović, Z.. (2022). Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion. in Food Chemistry: X
Elsevier Ltd.., 15, 100370.
https://doi.org/10.1016/j.fochx.2022.100370

Pavlović N, Mijalković J, Đorđević V, Pecarski D, Bugarski B, Knežević-Jugović Z. Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion. in Food Chemistry: X. 2022;15:100370.
doi:10.1016/j.fochx.2022.100370 .

Pavlović, Neda, Mijalković, Jelena, Đorđević, Verica, Pecarski, Danijela, Bugarski, Branko, Knežević-Jugović, Zorica, "Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion" in Food Chemistry: X, 15 (2022):100370,
https://doi.org/10.1016/j.fochx.2022.100370 . .

TY  - JOUR
AU  - Jovanović, Aleksandra A.
AU  - Vajić, Una-Jovana V.
AU  - Mijin, Dušan Ž.
AU  - Zdunić, Gordana M.
AU  - Šavikin, Katarina P.
AU  - Branković, Suzana
AU  - Kitić, Dušanka
AU  - Bugarski, Branko M.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5194
AB  - In the present study, polyphenol extraction from Thymus serpyllum by-product (herbal dust) in microwave reactor was optimized through varying extraction time, ethanol concentration and solid-to-solvent ratiovia determination of total polyphenol and flavonoid contents, and antioxidant activity, using response surface methodology and a central composite design. Total polyphenol and flavonoids contents, as well as antioxidant activity of the extracts were statistically significantly affected by ethanol concentration and solid-to-solvent ratio. However, extraction time had statistically significant influence only on polyphenol yield. The extraction conditions that maximized polyphenol recovery were 48% ethanol and 0.0402 g/mL solid-to-solvent ratio during 86 s of microwave-assisted extraction, and under these conditions total polyphenol content was estimated to be 57.2 mg of gallic acid equivalents (GAE)/L, while measured value was 58.1 ± 3.2 mg GAE/L. Polyphenol content (HPLC analysis), antioxidant, antimicrobial and antispasmodic potential of the extract prepared under these optimal extraction conditions were evaluated. Rosmarinic acids, luteolin 7-O-glucuronide, caffeic acid, apigenin glucuronide, 6-hydroxyluteolin 7-O-glucoside, 6,8-di-C-glucosylapigenin, and traces of chlorogenic acid were quantified in the extract (descending order of content). The selected extract inhibited 95% ± 0.9 of lipid peroxidation in β-Carotene and linoleic acid emulsion. It was more effective against tested Gram-positive bacteria than against Gram-negative and showed dose-dependent antispasmodic activity on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions. Microwave-assisted extraction could be selected as a successful technique for polyphenol extraction from wild thyme by-product. Biological activities shown in the study open the possibility of potential application of the extract in pharmaceutical, food, functional food and cosmetic products
PB  - Elsevier GmbH
T2  - Journal of Applied Research on Medicinal and Aromatic Plants
T1  - Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract
SP  - 100417
VL  - 31
DO  - 10.1016/j.jarmap.2022.100417
ER  - 

@article{
author = "Jovanović, Aleksandra A. and Vajić, Una-Jovana V. and Mijin, Dušan Ž. and Zdunić, Gordana M. and Šavikin, Katarina P. and Branković, Suzana and Kitić, Dušanka and Bugarski, Branko M.",
year = "2022",
abstract = "In the present study, polyphenol extraction from Thymus serpyllum by-product (herbal dust) in microwave reactor was optimized through varying extraction time, ethanol concentration and solid-to-solvent ratiovia determination of total polyphenol and flavonoid contents, and antioxidant activity, using response surface methodology and a central composite design. Total polyphenol and flavonoids contents, as well as antioxidant activity of the extracts were statistically significantly affected by ethanol concentration and solid-to-solvent ratio. However, extraction time had statistically significant influence only on polyphenol yield. The extraction conditions that maximized polyphenol recovery were 48% ethanol and 0.0402 g/mL solid-to-solvent ratio during 86 s of microwave-assisted extraction, and under these conditions total polyphenol content was estimated to be 57.2 mg of gallic acid equivalents (GAE)/L, while measured value was 58.1 ± 3.2 mg GAE/L. Polyphenol content (HPLC analysis), antioxidant, antimicrobial and antispasmodic potential of the extract prepared under these optimal extraction conditions were evaluated. Rosmarinic acids, luteolin 7-O-glucuronide, caffeic acid, apigenin glucuronide, 6-hydroxyluteolin 7-O-glucoside, 6,8-di-C-glucosylapigenin, and traces of chlorogenic acid were quantified in the extract (descending order of content). The selected extract inhibited 95% ± 0.9 of lipid peroxidation in β-Carotene and linoleic acid emulsion. It was more effective against tested Gram-positive bacteria than against Gram-negative and showed dose-dependent antispasmodic activity on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions. Microwave-assisted extraction could be selected as a successful technique for polyphenol extraction from wild thyme by-product. Biological activities shown in the study open the possibility of potential application of the extract in pharmaceutical, food, functional food and cosmetic products",
publisher = "Elsevier GmbH",
journal = "Journal of Applied Research on Medicinal and Aromatic Plants",
title = "Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract",
pages = "100417",
volume = "31",
doi = "10.1016/j.jarmap.2022.100417"
}

Jovanović, A. A., Vajić, U. V., Mijin, D. Ž., Zdunić, G. M., Šavikin, K. P., Branković, S., Kitić, D.,& Bugarski, B. M.. (2022). Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract. in Journal of Applied Research on Medicinal and Aromatic Plants
Elsevier GmbH., 31, 100417.
https://doi.org/10.1016/j.jarmap.2022.100417

Jovanović AA, Vajić UV, Mijin DŽ, Zdunić GM, Šavikin KP, Branković S, Kitić D, Bugarski BM. Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract. in Journal of Applied Research on Medicinal and Aromatic Plants. 2022;31:100417.
doi:10.1016/j.jarmap.2022.100417 .

Jovanović, Aleksandra A., Vajić, Una-Jovana V., Mijin, Dušan Ž., Zdunić, Gordana M., Šavikin, Katarina P., Branković, Suzana, Kitić, Dušanka, Bugarski, Branko M., "Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract" in Journal of Applied Research on Medicinal and Aromatic Plants, 31 (2022):100417,
https://doi.org/10.1016/j.jarmap.2022.100417 . .

TY  - JOUR
AU  - Bošković, Stefan A.
AU  - Karač, Aleksandar
AU  - Vrhovac, Slobodan B.
AU  - Belić, Aleksandar
AU  - Bugarski, Branko
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5148
AB  - A perfect dielectric model was incorporated into the OpenFOAM® software and used for investigation and, possibly, improvements of electrohydrodynamic calculations. Two different sets of numerical simulations were analyzed, in which two different fluids were present. The first set was one-dimensional, while in the second, a drop of one fluid was surrounded by the other fluid. It is shown that oscillations and possibly artificial generation of a curl of the electric field strength can be observed at applying certain expressions or calculation strategies, which can be thus abandoned. Usage of dynamic meshes, at least those present in the used software, and of limiters for the gradient of the electric field strength can lead to large numerical errors. It is also shown that usage of certain cell face values could improve the results. An electric Courant number was derived by dimensional analysis, and it could be suggested for future calculations. Conclusions made in this paper are expected to be transferable to other more complicated models.
AB  - Model idealnog dielektrika je uključen u programski paket OpenFOAM® (OpenFOAM Foundation, UK) i korišćen za ispitivanje i moguće poboljšavanje elektrohidrodinamičkih proračuna. Analizirana su dva različita seta numeričkih simulacija, u kojima su bila modelovana dva različita fluida. Prvi set je bio jednodimenzionalan dok je u drugom setu kap jednog fluida bila okružena drugim fluidom. U radu je pokazano da se određeni izrazi ili strategije izračunavanja mogu odbaciti usled pojave oscilacija i mogućeg veštačkog stvaranja rotora jačine električnog polja. Korišćenje pokretnih mreža, barem onih prisutnih u programskom paketu OpenFOAM®, i limitera za gradijent jačine električnog polja mogu dovesti do velikih numeričkih grešaka. Takođe je pokazano da bi korišćenje određenih vrednosti sa površi ćelija moglo poboljšati rezultate. Izraz za električni Kuronov broj je izveden dimenzionom analizom i mogao bi se preporučiti za buduće proračune. Očekuje se da su zaključci iz ovog rada prenosivi na druge, komplikovanije modele.
PB  - Savez hemijskih inženjera Srbije
T2  - Hemijska industrija
T1  - Investigation of electrohydrodynamic calculations
T1  - Ispitivanje elektrohidrodinamičkih proračuna
EP  - 74
IS  - 2
SP  - 65
VL  - 76
DO  - 10.2298/HEMIND211110010B
ER  - 

@article{
author = "Bošković, Stefan A. and Karač, Aleksandar and Vrhovac, Slobodan B. and Belić, Aleksandar and Bugarski, Branko",
year = "2022",
abstract = "A perfect dielectric model was incorporated into the OpenFOAM® software and used for investigation and, possibly, improvements of electrohydrodynamic calculations. Two different sets of numerical simulations were analyzed, in which two different fluids were present. The first set was one-dimensional, while in the second, a drop of one fluid was surrounded by the other fluid. It is shown that oscillations and possibly artificial generation of a curl of the electric field strength can be observed at applying certain expressions or calculation strategies, which can be thus abandoned. Usage of dynamic meshes, at least those present in the used software, and of limiters for the gradient of the electric field strength can lead to large numerical errors. It is also shown that usage of certain cell face values could improve the results. An electric Courant number was derived by dimensional analysis, and it could be suggested for future calculations. Conclusions made in this paper are expected to be transferable to other more complicated models., Model idealnog dielektrika je uključen u programski paket OpenFOAM® (OpenFOAM Foundation, UK) i korišćen za ispitivanje i moguće poboljšavanje elektrohidrodinamičkih proračuna. Analizirana su dva različita seta numeričkih simulacija, u kojima su bila modelovana dva različita fluida. Prvi set je bio jednodimenzionalan dok je u drugom setu kap jednog fluida bila okružena drugim fluidom. U radu je pokazano da se određeni izrazi ili strategije izračunavanja mogu odbaciti usled pojave oscilacija i mogućeg veštačkog stvaranja rotora jačine električnog polja. Korišćenje pokretnih mreža, barem onih prisutnih u programskom paketu OpenFOAM®, i limitera za gradijent jačine električnog polja mogu dovesti do velikih numeričkih grešaka. Takođe je pokazano da bi korišćenje određenih vrednosti sa površi ćelija moglo poboljšati rezultate. Izraz za električni Kuronov broj je izveden dimenzionom analizom i mogao bi se preporučiti za buduće proračune. Očekuje se da su zaključci iz ovog rada prenosivi na druge, komplikovanije modele.",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija",
title = "Investigation of electrohydrodynamic calculations, Ispitivanje elektrohidrodinamičkih proračuna",
pages = "74-65",
number = "2",
volume = "76",
doi = "10.2298/HEMIND211110010B"
}

Bošković, S. A., Karač, A., Vrhovac, S. B., Belić, A.,& Bugarski, B.. (2022). Investigation of electrohydrodynamic calculations. in Hemijska industrija
Savez hemijskih inženjera Srbije., 76(2), 65-74.
https://doi.org/10.2298/HEMIND211110010B

Bošković SA, Karač A, Vrhovac SB, Belić A, Bugarski B. Investigation of electrohydrodynamic calculations. in Hemijska industrija. 2022;76(2):65-74.
doi:10.2298/HEMIND211110010B .

Bošković, Stefan A., Karač, Aleksandar, Vrhovac, Slobodan B., Belić, Aleksandar, Bugarski, Branko, "Investigation of electrohydrodynamic calculations" in Hemijska industrija, 76, no. 2 (2022):65-74,
https://doi.org/10.2298/HEMIND211110010B . .

TY  - CONF
AU  - Volić, Mina
AU  - Obradović, Nataša
AU  - Jovanović, Aleksandra
AU  - Bugarski, Branko
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6451
AB  - Cherry fruits are known for their wide range of nutritive and bioactive compounds, including
fibers, vitamins, and phytonutrients. Sweet cherry consumption has been associated with
several health benefits against human problems such as cardiovascular diseases, cancer,
diabetes, and Alzheimer’s disease. Particularly, cherries contain abundant phenolic compounds
with antioxidant, anti-inflammatory, and anticancer properties as they exert a protective role
against oxidative stress and free radical damages. However, there is not a specific method to
fully extract all phenolics and other compounds from sweet cherries. Thus, this study aimed to
optimize the extraction of cherries through different extraction procedures (maceration and
ultrasound-assisted extraction) using different solvents (50% EtOH with and without HCl, and
Tris buffer). The resulting cherry fruit extracts were investigated regarding their i) total phenol
content - according to the Folin-Ciocalteu colorimetric method, ii) total protein content -
evaluated using the Bradford protein assay, and iii) antioxidant activity – characterized by
ABTS, DPPH, and FRAP assays. The ultrasound-assisted extraction using 50% EtOH and HCl
(pH=2.3) demonstrated the best results for total phenol content (7.51 mg/L), DPPH inhibition
(23.7%), and FRAP reducing power (24.5 mmol/g). On the contrary, the highest ABTS
scavenging activity was noticed for maceration obtained extract with 50% EtOH and HCl (7.23
mg/mL). Finally, the best protein yield was obtained for cherries when extraction was
performed in 0.1M Tris buffer at pH=11.2 (0.23 mg/mL). The optimization methodology in this
work is used as a screening test for producing bioactive compounds-rich extracts for the food
industry
PB  - University of Novi Sad, Faculty of Technology Novi Sad
C3  - Book of Abstracts of the 2nd International Conference on Advanced Production and Processing
T1  - Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts
SP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6451
ER  - 

@conference{
author = "Volić, Mina and Obradović, Nataša and Jovanović, Aleksandra and Bugarski, Branko",
year = "2022",
abstract = "Cherry fruits are known for their wide range of nutritive and bioactive compounds, including
fibers, vitamins, and phytonutrients. Sweet cherry consumption has been associated with
several health benefits against human problems such as cardiovascular diseases, cancer,
diabetes, and Alzheimer’s disease. Particularly, cherries contain abundant phenolic compounds
with antioxidant, anti-inflammatory, and anticancer properties as they exert a protective role
against oxidative stress and free radical damages. However, there is not a specific method to
fully extract all phenolics and other compounds from sweet cherries. Thus, this study aimed to
optimize the extraction of cherries through different extraction procedures (maceration and
ultrasound-assisted extraction) using different solvents (50% EtOH with and without HCl, and
Tris buffer). The resulting cherry fruit extracts were investigated regarding their i) total phenol
content - according to the Folin-Ciocalteu colorimetric method, ii) total protein content -
evaluated using the Bradford protein assay, and iii) antioxidant activity – characterized by
ABTS, DPPH, and FRAP assays. The ultrasound-assisted extraction using 50% EtOH and HCl
(pH=2.3) demonstrated the best results for total phenol content (7.51 mg/L), DPPH inhibition
(23.7%), and FRAP reducing power (24.5 mmol/g). On the contrary, the highest ABTS
scavenging activity was noticed for maceration obtained extract with 50% EtOH and HCl (7.23
mg/mL). Finally, the best protein yield was obtained for cherries when extraction was
performed in 0.1M Tris buffer at pH=11.2 (0.23 mg/mL). The optimization methodology in this
work is used as a screening test for producing bioactive compounds-rich extracts for the food
industry",
publisher = "University of Novi Sad, Faculty of Technology Novi Sad",
journal = "Book of Abstracts of the 2nd International Conference on Advanced Production and Processing",
title = "Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts",
pages = "180",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6451"
}

Volić, M., Obradović, N., Jovanović, A.,& Bugarski, B.. (2022). Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts. in Book of Abstracts of the 2nd International Conference on Advanced Production and Processing
University of Novi Sad, Faculty of Technology Novi Sad., 180.
https://hdl.handle.net/21.15107/rcub_technorep_6451

Volić M, Obradović N, Jovanović A, Bugarski B. Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts. in Book of Abstracts of the 2nd International Conference on Advanced Production and Processing. 2022;:180.
https://hdl.handle.net/21.15107/rcub_technorep_6451 .

Volić, Mina, Obradović, Nataša, Jovanović, Aleksandra, Bugarski, Branko, "Optimization of Sweet Cherry Extraction: Evaluation of Total Phenol Content, Protein Content, and Antioxidant Activity of Extracts" in Book of Abstracts of the 2nd International Conference on Advanced Production and Processing (2022):180,
https://hdl.handle.net/21.15107/rcub_technorep_6451 .

TY  - JOUR
AU  - Jonović, Marko
AU  - Jugović, Branimir
AU  - Žuža, Milena
AU  - Ðorđević, Verica
AU  - Milašinović, Nikola
AU  - Bugarski, Branko
AU  - Knežević-Jugović, Zorica
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5165
AB  - The aim of this study was to investigate covalent immobilization of horseradish peroxidase (HRP) on magnetic nanoparticles (Mag) encapsulated in calcium alginate beads (MABs) for color degradation, combining easy and fast removal of biocatalyst from the reaction mixture due to its magnetic properties and strong binding due to surface alginate functional groups. MABs obtained by extrusion techniques were analyzed by optical microscopy, FEG-SEM and characterized regarding mechanical properties, magnetization and HRP binding. HRP with initial concentration of 10 mg/gcarrier was successfully covalently bonded on MABs (diameter ~1 mm, magnetite/alginate ratio 1:4), with protein loading of 8.9 mg/gcarrier, immobilization yield 96.9% and activity 32.8 U/g. Immobilized HRP on MABs (HRP-MABs) was then used to catalyze degradation of two anthraquinonic dyes, Acid Blue 225 (AB225) and Acid Violet 109 (AV109), as models for wastewater pollutants. HRP-MABs decolorized 77.3% and 76.1% of AV109 and AB225, respectively after 15 min under optimal conditions (0.097 mM H2O2, 200 mg of HRP-MABs (8.9 mg/gcarrier), 0.08 and 0.1 g/mg beads/dye ratio for AV109 and AB225, respectively). Biocatalyst was used for 7 repeated cycles retaining 75% and 51% of initial activity for AB225 and AV109, respectively, showing potential for use in large scale applications for colored wastewater treatment.
PB  - MDPI
T2  - Polymers
T1  - Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation
IS  - 13
SP  - 2614
VL  - 14
DO  - 10.3390/polym14132614
ER  - 

@article{
author = "Jonović, Marko and Jugović, Branimir and Žuža, Milena and Ðorđević, Verica and Milašinović, Nikola and Bugarski, Branko and Knežević-Jugović, Zorica",
year = "2022",
abstract = "The aim of this study was to investigate covalent immobilization of horseradish peroxidase (HRP) on magnetic nanoparticles (Mag) encapsulated in calcium alginate beads (MABs) for color degradation, combining easy and fast removal of biocatalyst from the reaction mixture due to its magnetic properties and strong binding due to surface alginate functional groups. MABs obtained by extrusion techniques were analyzed by optical microscopy, FEG-SEM and characterized regarding mechanical properties, magnetization and HRP binding. HRP with initial concentration of 10 mg/gcarrier was successfully covalently bonded on MABs (diameter ~1 mm, magnetite/alginate ratio 1:4), with protein loading of 8.9 mg/gcarrier, immobilization yield 96.9% and activity 32.8 U/g. Immobilized HRP on MABs (HRP-MABs) was then used to catalyze degradation of two anthraquinonic dyes, Acid Blue 225 (AB225) and Acid Violet 109 (AV109), as models for wastewater pollutants. HRP-MABs decolorized 77.3% and 76.1% of AV109 and AB225, respectively after 15 min under optimal conditions (0.097 mM H2O2, 200 mg of HRP-MABs (8.9 mg/gcarrier), 0.08 and 0.1 g/mg beads/dye ratio for AV109 and AB225, respectively). Biocatalyst was used for 7 repeated cycles retaining 75% and 51% of initial activity for AB225 and AV109, respectively, showing potential for use in large scale applications for colored wastewater treatment.",
publisher = "MDPI",
journal = "Polymers",
title = "Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation",
number = "13",
pages = "2614",
volume = "14",
doi = "10.3390/polym14132614"
}

Jonović, M., Jugović, B., Žuža, M., Ðorđević, V., Milašinović, N., Bugarski, B.,& Knežević-Jugović, Z.. (2022). Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation. in Polymers
MDPI., 14(13), 2614.
https://doi.org/10.3390/polym14132614

Jonović M, Jugović B, Žuža M, Ðorđević V, Milašinović N, Bugarski B, Knežević-Jugović Z. Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation. in Polymers. 2022;14(13):2614.
doi:10.3390/polym14132614 .

Jonović, Marko, Jugović, Branimir, Žuža, Milena, Ðorđević, Verica, Milašinović, Nikola, Bugarski, Branko, Knežević-Jugović, Zorica, "Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation" in Polymers, 14, no. 13 (2022):2614,
https://doi.org/10.3390/polym14132614 . .